Miracle-dz/UniFAR-FTUs huggingface dataset:
Challenging old microbiological treasures for natural compound biosynthesis capacity

Strain collections are a treasure chest of numerous valuable and taxonomically validated bioresources. The Leibniz Institute DSMZ is one of the largest and most diverse microbial strain collections worldwide, with a long tradition of actinomycetes research. Actinomycetes, especially the genus Streptomyces, are renowned as prolific producers of antibiotics and many other bioactive natural products. In light of this, five Streptomyces strains, DSM 40971T, DSM 40484T, DSM 40713T, DSM 40976T, and DSM 40907T, which had been deposited a long time ago without comprehensive characterization, were the subject of polyphasic taxonomic studies and genome mining for natural compounds based on in vitro and in silico analyses. Phenotypic, genetic, and phylogenomic studies distinguished the strains from their closely related neighbors. The digital DNA–DNA hybridization and average nucleotide identity values between the five strains and their close, validly named species were below the threshold of 70% and 95%–96%, respectively, determined for prokaryotic species demarcation. Therefore, the five strains merit being considered as novel Streptomyces species, for which the names Streptomyces kutzneri sp. nov., Streptomyces stackebrandtii sp. nov., Streptomyces zähneri sp. nov., Streptomyces winkii sp. nov., and Streptomyces kroppenstedtii sp. nov. are proposed. Bioinformatics analysis of the genome sequences of the five strains revealed their genetic potential for the production of secondary metabolites, which helped identify the natural compounds cinerubin B from strain DSM 40484T and the phosphonate antibiotic phosphonoalamide from strain DSM 40907T and highlighted strain DSM 40976T as a candidate for regulator-guided gene cluster activation due to the abundance of numerous “Streptomyces antibiotic regulatory protein” (SARP) genes.
Calculation Program for Isochron Ages of Rb-Sr and Sm-Nd Systems Using Personal Computer

Calculation program for isochron ages of Rb-Sr and Sm-Nd systems based on York (1966) is presented. This paper discusses calculated method and its BASIC program. The program is approved by test data, spread sheet program and published data. In conclusion , calculated results by the program is sufficiently confided.
Adult Attachment Questionnaire: evidence of validity in a sample of IPVAW offenders

Introduction Adult attachment can be understood as a cognitive and emotional system concerning oneself and others, based on previous attachment experiences throughout life. This system automatically affects relationships with others. Because of its importance in the interpersonal domain, it has been studied on numerous occasions in research on intimate partner violence. The aim of this study was to obtain evidence of validity of the Adult Attachment Questionnaire (AAQ) in a sample of 331 men convicted of intimate partner violence against women (IPVAW). Methods The AAQ assesses adult attachment style in four dimensions that, together, yield four attachment categories. A psychometric analysis was performed, including reliability analysis and confirmatory factor analysis of the items, which ratified the factorial structure of the questionnaire. For a correct fit of the model, it was necessary to eliminate 4 of the items from the original scale. Results A latent profile analysis was also carried out, which identified four attachment styles: secure, preoccupied/anxious, avoidant/dismissing and fearful. Reliability indices were adequate. In general, the attachment profiles obtained ranges and means similar to those found in the general population study. The distribution of attachment styles was not equal: 50.57% of the participants presented secure attachment, 4.57% avoidant/dismissing attachment, 36.9% preoccupied/anxious, and 8.57% fearful. Discussion In conclusion, a valid and reliable instrument was determined based on the original AAQ scale to measure attachment in men convicted of IPVAW.
Parents’ perceptive regarding the effect of internet use on students with and without learning difficulties

In the recent digital era, the use of the internet worldwide has become vital in almost all fields of life, and no one can deny its significant impact on activities of different age groups. The current study investigated the effect of internet usage on students with/without learning difficulties in Jordanian primary schools from point of view of their parents. For this purpose, a questionnaire consisting of 21 items was built by the researchers including these areas: social interactions, communication, health, entertainment, learning, academic activities, behavior, and security. The targeted population was the parents of students with/without learning difficulties in public and private schools. The findings indicated that parents perceive a moderate level of effect of internet usage on their children’s most activities except entertainment and security which have a high impact. Moreover, parents of students with learning difficulties perceive a positive impact of the internet in enhancing their children’s learning abilities.
Proton-Conducting Membranes from Polyphenylenes Containing Armstrong’s Acid

This study demonstrates the use of 1,5-naphthalenedisulfonic acid as a suitable building block for the efficient and economic preparation of alternating sulfonated polyphenylenes with high ion-exchange capacity (IEC) via Suzuki polycondensation. Key to large molar masses is the use of an all-meta-terphenyl comonomer instead of m-phenyl, the latter giving low molar masses and brittle materials. A protection/deprotection strategy for base-stable neopentyl sulfonates is successfully implemented to improve the solubility and molar mass of the polymers. Solution-based deprotection of polyphenylene neopentyl sulfonates at 150 °C in dimethylacetamide eliminates isopentylene quantitatively, resulting in membranes with high IEC (2.93 mequiv/g) and high proton conductivity (σ = 138 mS/cm). Water solubility of these copolymers with high IEC requires thermal cross-linking to prevent their dissolution under operating conditions. By balancing the temperature and time of the cross-linking process, water uptake can be restricted to 50 wt %, retaining an IEC of 2.33 mequiv/g and a conductivity of 85 mS/cm. Chemical stability is addressed by treatment of the membranes under Fenton’s conditions and by considering barrier heights for desulfonation using density functional theory (DFT) calculations. The DFT results suggest that 1,5-disulfonated naphthalenes are at least as stable as sulfonated polyphenylenes against desulfonation.
Photodynamic Therapy for Eye, Ear, Laryngeal Area, and Nasal and Oral Cavity Diseases: A Review

Simple Summary Photodynamic therapy (PDT) has emerged as a versatile treatment option for head and neck disorders. The purpose of our review was to provide an overview of the use of PDT therapy in a variety of diseases. The use of PDT therapy has recently emerged as an innovative and effective method of cancer treatment. However, more and more studies are showing that it is also promising for treating a variety of non-cancerous lesions, providing a minimally invasive alternative for certain conditions. The same following principles apply: the application of a photosensitizer, exposure to light, and then the destruction of abnormal cells. In our work, we highlighted the importance of photodynamic therapy and its valuable treatment as an option for a variety of diseases of the head and neck area, not just cancer. With this, we want to encourage researchers to more widely use photodynamic therapy in head and neck disorders. Abstract Photodynamic therapy (PDT) has emerged as a promising modality for the treatment of various diseases. This non-invasive approach utilizes photosensitizing agents and light to selectively target and destroy abnormal cells, providing a valuable alternative to traditional treatments. Research studies have explored the application of PDT in different areas of the head. Research is focusing on a growing number of new developments and treatments for cancer. One of these methods is PDT. Photodynamic therapy is now a revolutionary, progressive method of cancer therapy. A very important feature of PDT is that cells cannot become immune to singlet oxygen. With this therapy, patients can avoid lengthy and costly surgeries. PDT therapy is referred to as a safe and highly selective therapy. These studies collectively highlight the potential of PDT as a valuable therapeutic option in treating the head area. As research in this field progresses, PDT may become increasingly integrated into the clinical management of these conditions, offering a balance between effectiveness and minimal invasiveness.
Designing Thieno[3,4-c]pyrrole-4,6-dione Core-Based, A2–D–A1–D–A2-Type Acceptor Molecules for Promising Photovoltaic Parameters in Organic Photovoltaic Cells

Nonfullerene-based organic solar cells can be utilized as favorable photovoltaic and optoelectronic devices due to their enhanced life span and efficiency. In this research, seven new molecules were designed to improve the working efficiency of organic solar cells by utilizing a terminal acceptor modification approach. The perceived A2–D–A1–D–A2 configuration-based molecules possess a lower band gap ranging from 1.95 to 2.21 eV compared to the pre-existing reference molecule (RW), which has a band gap of 2.23 eV. The modified molecules also exhibit higher λmax values ranging from 672 to 768 nm in the gaseous and 715–839 nm in solvent phases, respectively, as compared to the (RW) molecule, which has λmax values at 673 and 719 nm in gas and chloroform medium, respectively. The ground state geometries, molecular planarity parameter, and span of deviation from the plane were analyzed to study the planarity of all of the molecules. The natural transition orbitals, the density of state, molecular electrostatic potential, noncovalent interactions, frontier molecular orbitals, and transition density matrix analysis of all studied molecules were executed to validate the optoelectronic properties of these molecules. Improved charge mobilities and dipole moments were observed, as newly designed molecules possessed lower internal reorganization energies. The open circuit voltage (Voc) of W4, W5, W6, and W7 among newly designed molecules was improved as compared to the reference molecule. These results elaborate on the superiority of these novel-designed molecules over the pre-existing (RW) molecule as potential blocks for better organic solar cell applications.
in Satellite-Ground Laser Communications using Satellite Images

The sites selection of optical ground stations (OGSs) in satellite-ground laser communications and the effects applied two types of diversity schemes are examined quantitatively. The existence of interception of the laser beam by clouds, which causes communication quality degradation is judged using the dot values of meteorological satellite images. The proposed sites are chosen in consideration of the locality of clouds distribution, and the existing terrestrial networks. Site- and time-diversity are applied to the proposed sites, and the achievement scheme of not less than 99.9% availability is examined.
Interpretation of gas chimney in the Maari 3D field of southern Taranaki Basin, New Zealand

The interpretation of Maari 3D seismic data of Southern Taranaki Basin, using chimney analysis tool, brings out the vertical gas migration pathways from the source through the reservoir traps all the way to the surface. This correlates well with the available drilling results. The chimney effects also exhibit good correlations with seismic attributes when displayed over the horizon slices mapped over the formation tops. This study provides important inputs in understanding the petroleum system of the study region and acts as a preventive measure for mitigating geo-hazards in future drilling.
Scale development and validation of perimenopausal women disability index in the workplace

Background Menopausal disorders include obscure symptomatology that greatly reduce work productivity among female workers. Quantifying the impact of menopause-related symptoms on work productivity is very difficult because no such guidelines exist to date. We aimed to develop a scale of overall health status for working women in the perimenopausal period. Methods In September, 2021, we conducted an Internet web survey which included 3,645 female workers aged 45–56 years in perimenopausal period. We asked the participants to answer 76 items relevant to menopausal symptomatology, that were created for this study and performed exploratory and confirmatory factor analyses for the scale development. Cronbach’s alpha, receiver operating characteristic analysis, and logistic regression analysis were used to verify the developed scale. Results Approximately 85% participants did not have menstruation or disrupted cycles. Explanatory factor analysis using the maximum likelihood method and Promax rotation identified 21 items with a four-factor structure: psychological symptoms (8 items, α = 0.96); physiological symptoms (6 items, alpha = 0.87); sleep difficulty (4 items, alpha = 0.92); human relationship (3 items, alpha = 0.92). Confirmatory factor analyses found excellent model fit for the four-factor model (RMSR = 0.079; TLI = 0.929; CFI = 0.938). Criterion and concurrent validity were confirmed with high correlation coefficients between each of the four factors, previously validated menopausal symptom questionnaire, and Copenhagen Burnout Inventory scales, respectively (all ps < 0.0001). The developed scale was able to predict absenteeism with 78% sensitivity, 58% specificity, and an AUC of 0.727 (95%CI: 0.696–0.757). Higher scores of each factor as well as total score of the scale were more likely to be associated with work absence experience due to menopause-related symptoms even after adjusting for Copenhagen Burnout Inventory subscales (all ps < 0.0001). Conclusion We found that the developed scale has high validity and reliability and could be a significant indicator of absenteeism for working women in perimenopausal period. Supplementary information The online version contains supplementary material available at https://doi.org/10.1265/ehpm.23-00239.
Genome-Wide Association Study and Prediction of Tassel Weight of Tropical Maize Germplasm in Multi-Parent Population

Tassel weight (TW) is a crucial agronomic trait that significantly affects pollen supply and grain yield development in maize breeding. To improve maize yield and develop new varieties, a comprehensive understanding of the genetic mechanisms underlying tassel weight is essential. In this study, tropical maize inbred lines, namely CML312, CML373, CML444, and YML46, were selected as female parents and crossed with the elite maize inbred line Ye107, which served as the common male parent, to develop a multi-parent population comprising four F8 recombinant inbred line (RIL) subpopulations. Using 6616 high-quality single nucleotide polymorphism (SNP) markers, we conducted genome-wide association analysis (GWAS) and genomic selection (GS) on 642 F8 RILs in four subpopulations across three different environments. Through GWAS, we identified 16 SNPs that were significantly associated with TW, encompassing two stable loci expressed across multiple environments. Furthermore, within the candidate regions of these SNPs, we discovered four novel candidate genes related to TW, namely Zm00001d044362, Zm00001d011048, Zm00001d011049, and Zm00001d031173 distributed on chromosomes 1, 3, and 8, which have not been previously reported. These genes are involved in processes such as signal transduction, growth and development, protein splicing, and pollen development, all of which play crucial roles in inflorescence meristem development, directly affecting TW. The co-localized SNP, S8_137379725, on chromosome 8 was situated within a 16.569 kb long terminal repeat retrotransposon (LTR-RT), located 22.819 kb upstream and 26.428 kb downstream of the candidate genes (Zm00001d011048 and Zm00001d011049). When comparing three distinct GS models, the BayesB model demonstrated the highest accuracy in predicting TW. This study establishes the theoretical foundation for future research into the genetic mechanisms underlying maize TW and the efficient breeding of high-yielding varieties with desired tassel weight through GS.
Differences in Supervision on Peer Learning Wards: A Pilot Survey of the Supervisor’s Perspective

Introduction Multiple students are placed on clinical wards simultaneously due to increasing student numbers. This has the potential to create stress for the supervisor and reduce quality of student learning. Peer learning as a pedagogical framework to supervise multiple students has been widely shown to have advantages for the students by developing teaching skills, team collaboration, and independence. However, whether peer learning impacts the characteristics of supervision and the experience of the supervisor is less understood. It is unknown whether wards that use peer learning as a pedagogical framework (peer learning wards) are any different compared to wards that do not (non-peer learning wards), from the supervisor’s perspective. Methods We aimed to develop and pilot test a questionnaire to compare peer-learning wards and non-peer learning wards from the supervisor’s perspective. We used the AMEE 7-step guide to develop questions investigating supervision, the learning environment and satisfaction. We piloted the questionnaire with 46 nurse supervisors working on inpatient hospital wards in Stockholm, Sweden. We compared answers from peer learning with non-peer learning wards. We used Orthogonal Projections to Latent Structures (OPLS) discriminant analysis to show what differed between the wards. Results Peer learning wards compared to non-peer learning wards had more student-centred activities, the physical space had more adaptations for students, more support available to the supervisor, and supervisors perceived greater overall satisfaction with the quality of education and with the ward as a whole. The variables that had most influence on the discrimination between the two ward types related to peer learning activities and perceptions (p=0.0034). Conclusion This pilot study shows that peer learning wards differ compared to non-peer learning wards regarding peer learning activities and perceptions among supervisors. Our questionnaire needs to be distributed on a larger scale to validate our findings and explore further the way in which the pedagogical framework and peer learning can affect supervision and satisfaction.
Endometrial transcriptome profiling of patients with recurrent implantation failure during hormone replacement therapy cycles

Background The molecular mechanisms underlying window of implantation (WOI) displacement in patients with recurrent implantation failure (RIF) remain unclear. This study aims to explore the transcriptomic signatures of endometrium with normal and displaced WOIs and to identify the causes of endometrial receptivity (ER) abnormalities and WOI displacement in RIF patients. Methods In this study, 40 RIF patients were recruited and underwent personalized embryo transfer (pET) guided by the predicted results of endometrial receptivity diagnosis (ERD) model. Transcriptome analysis of endometrium from patients with clinical pregnancies after pET was performed to identify differentially expressed genes (DEGs) associated with WOI displacement. Gene expression data from HRT and natural cycle endometrium were compared to identify specific gene expression patterns of ER-related genes during WOI. Results The ERD results indicated that 67.5% of RIF patients (27/40) were non-receptive in the conventional WOI (P+5) of the HRT cycle. The clinical pregnancy rate in RIF patients improved to 65% (26/40) after ERD-guided pET, indicating the effectiveness of transcriptome-based WOI prediction. Among the 26 patients with clinical pregnancy, the gene expression profiles of P+5 endometrium from advanced (n=6), normal (n=10) and delayed (n=10) WOI groups were significantly different from each other. Furthermore, 10 DEGs identified among P+5 endometrium of 3 groups were involved in immunomodulation, transmembrane transport and tissue regeneration, which could accurately classify the endometrium with different WOIs. Additionally, a large number of ER-related genes showed significant correlation and similar gene expression patterns in P+3, P+5, and P+7 endometrium from HRT cycles and LH+5, LH+7, and LH+9 endometrium from natural cycles. Conclusion Our study shows that ER-related genes share similar gene expression patterns during WOI in both natural and HRT cycles, and their aberrant expression is associated with WOI displacements. The improvement of pregnancy outcomes in RIF patients by adjusting ET timing according to ERD results demonstrates the importance of transcriptome-based endometrial receptivity assessment and the clinical efficiency of ERD model.
The Need for Status, Organizational Citizenship Behavior, and Overall Evaluation: Focusing on the Moderating Effects of Political Skill and Task Visibility

Drawing on the costly signaling theory (CST), this study examined the need for status as a hidden motive to increase organizational citizenship behavior (OCB) and result in the focal employee’s overall evaluation. Moreover, as the activating cues, this study considered political skill as an individual characteristic and task visibility as a situational factor in the relationship between the need for status, OCB, and overall evaluation. To test these predictions, we obtained and analyzed 299 questionnaire responses from pairs of subordinates and supervisors in various South Korean industries. The analytical results supported our hypotheses that the need for status increased OCB, resulting in high performance appraisal. Moreover, task visibility positively moderated the relationship between the need for status and overall evaluation, which was mediated by OCB. However, political skill exhibited a negative moderation effect on this mediation process. These findings have important theoretical and practical implications, and they also highlight directions for future research.
The Roles of Septins in Regulating Fission Yeast Cytokinesis

Cytokinesis is required to separate two daughter cells at the end of mitosis, and septins play crucial roles in many aspects of cytokinesis. While septins have been intensively studied in many model organisms, including the budding yeast Saccharomyces cerevisiae, septins have been relatively less characterized in the fission yeast Schizosaccharomyces pombe, which has proven to be an excellent model organism for studying fundamental cell biology. In this review, we summarize the findings of septins made in fission yeasts mainly from four aspects: the domain structure of septins, the localization of septins during the cell cycle, the roles of septins in regulating cytokinesis, and the regulatory proteins of septins.