Miracle-dz/UniFAR-FTUs huggingface dataset:
Nano-zinc oxide foliar application at the tuber initiation stage enhances the starch synthesis and zinc bioaccumulation in potato tubers

Crops cultivated on alkaline soils are normally deficient in micronutrients, therefore among others, zinc deficiency is a widespread problem in Pakistan. By using a novel approach, we have shown that agronomic biofortification of potato tubers can be achieved through application of nano-zinc oxide during the tuber initiation stage. Field experimentation was performed during the potato growing season (2021-2022) with five different concentrations of nano-zinc oxide (0, 25, 50, 100, and 250 mg L-1) applied to 55-d old plants at tuber initiation stage, and after 40 days of spray, potato tubers were harvested for the analysis of various biochemical and agronomic traits and changes in bioavailable concentration of Zn in potato tubers. An increase in growth and yield traits was recorded due to spray of 25 and 50 mg L-1 nano-zinc oxide. Starch fraction in tubers was increased in response to all nano-zinc oxide concentrations. Interestingly, over 50% increase in starch was recorded due to application of 25 mg L-1 nano-zinc oxide, while higher doses up to 250 mg L-1 increased tuber starch by ~34%. Soluble sugars increased progressively up to 250 mg L-1 reaching maximum values than the control. Above all, maximum concentration of Zn in potato tuber was recorded in response to 250 mg L-1 spray of nano-zinc oxide ranging up to 58.2 - 65.5 µg g-1 dry weight. Overall, exogenous application of nano-zinc oxide to potato plants can improve potato tuber quality by enhancing starch contents and contributing to translocation of Zn in potato tubers leading to agronomic biofortification.
EVALUATION OF ANCHORAGE TECHNIQUE ON THE ADAPTION OF PARTIAL DENTURE FRAMEWORK (COMPARATIVE IN VITRO STUDY)

The cast partial removable partial denture must be well fitted on the master dental cast and also on the oral tissues to be more successful and fulfill its functions. The objective of this in-vitro study was to evaluate the effect of anchorage on the accuracy of fit in removable partial denture framework. The study consisted of fourteen maxillary partially edentulous refractory casts Class III Kennedy classification modification 1which were divided equally into two groups group I test group (Anchorage technique) and group II control group (Conventional technique). Seven cobalt chrome removable partial denture frameworks were constructed for each group, one using conventional technique with performed wax pattern on a refractory casts and other seven cobalt chrome removable partial denture frameworks were constructed using anchorage technique including four holes for anchorage on the refractory casts. Both patterns were invested and casted under the same circumstances using conventional standardized technique.The resulting metallic removable partial denture frameworks were evaluated to check the fitness of palatal strap the major connector on the master metal cast using digital micrometer.The statistical results of this study showed significant reduction in the thickness (gap) between the palatal strap major connector and its related site among group I (Anchorage) when compared with group II(Conventional) which indicating more accurate fit for group I. Furthermore, all the frameworks which were constructed by anchorage technique revealed more gap reduction centrally and anteriorly than peripherally and posteriorly.
Bioenergetic and Metagenomic Analysis of Microbial Sulfur Cycling at Borup Fiord Pass Glacier, Canadian High Arctic

Flavobacteriaceae genera play an important role in the global degradation of complex organic carbon. I isolated two Flavobacteriaceae members from S deposits on the surface of Borup Fiord Pass Glacier, Canadian High Arctic, which may also have a significant sulfurcycling role. Isolate Flavobacterium sp. BF09-06_kw11. 51, a representative of the species-level Flavobacterium that dominates surface layers of the glacial S deposits, oxidized thiosulfate to sulfate but did not conserve energy from this reaction. In contrast, Gillisia sp. BF64A_kw10. 4 produced novel S biomineralized structures. It was not determined whether the Gillisia sp. BF64A_kw10. 4 isolate oxidized the sulfide to S or simply biomineralized S that formed by abiotic sulfide oxidation, but in either case this represents a previously-unknown capability for Flavobacteriaceae, Both isolates were able to assimilate 14C-labeled bicarbonate, although the reason for this capability was not determined.
DNA barcoding continues to identify endangered species of shark sold as food in a globally significant shark fin trade hub

Shark fins are a delicacy consumed throughout Southeast Asia. The life history characteristics of sharks and the challenges associated with regulating fisheries and the fin trade make sharks particularly susceptible to overfishing. Here, we used DNA barcoding techniques to investigate the composition of the shark fin trade in Singapore, a globally significant trade hub. We collected 505 shark fin samples from 25 different local seafood and Traditional Chinese Medicine shops. From this, we identified 27 species of shark, three species are listed as Critically Endangered, four as Endangered and ten as Vulnerable by the International Union for Conservation of Nature (IUCN). Six species are listed on CITES Appendix II, meaning that trade must be controlled in order to avoid utilization incompatible with their survival. All dried fins collected in this study were sold under the generic term “shark fin”; this vague labelling prevents accurate monitoring of the species involved in the trade, the effective implementation of policy and conservation strategy, and could unwittingly expose consumers to unsafe concentrations of toxic metals. The top five most frequently encountered species in this study are Rhizoprionodon acutus, Carcharhinus falciformis, Galeorhinus galeus, Sphyrna lewini and Sphyrna zygaena. Accurate labelling that indicates the species of shark that a fin came from, along with details of where it was caught, allows consumers to make an informed choice on the products they are consuming. Doing this could facilitate the avoidance of species that are endangered, and similarly the consumer can choose not to purchase species that are documented to contain elevated concentrations of toxic metals.
Nanotechnological synergy of mangiferin and curcumin in modulating PI3K/Akt/mTOR pathway: a novel front in ovarian cancer precision therapeutics

Background: Ovarian cancer, colloquially termed the “silent killer” among gynecological malignancies, remains elusive due to its often-asymptomatic progression and diagnostic challenges. Central to its pathogenesis is the overactive PI3K/Akt/mTOR signaling pathway, responsible for various cellular functions, from proliferation to survival. Within this context, the phytochemical compounds mangiferin (derived from Mangifera indica) and curcumin (from Curcuma longa) stand out for their potential modulatory effects. However, their inherent bioavailability challenges necessitate innovative delivery systems to maximize therapeutic benefits. Objective: This study seeks to synergize the merits of nanotechnology with the therapeutic properties of mangiferin and curcumin, aiming to bolster their efficacy against ovarian cancer. Methods: Employing specific nanotechnological principles, we engineered exosomal and liposomal nano-carriers for mangiferin and curcumin, targeting the PI3K/Akt/mTOR pathway. Molecular docking techniques mapped the interactions of these phytochemicals with key proteins in the pathway, analyzing their binding efficiencies. Furthermore, molecular dynamics simulations, spanning 100 nanoseconds, verified these interactions, with additional computational methodologies further validating our findings. The rationale for the 100 nanoseconds time span lies in its sufficiency to observe meaningful protein-ligand interactions and conformational changes. Notably, liposomal technology provided an enhancement in drug delivery by protecting these compounds from degradation, allowing controlled release, and improving cellular uptake. Results: Our computational investigations demonstrated notable binding affinities of mangiferin and curcumin: PI3K at −11.20 kcal/mol, Akt at −15.16 kcal/mol, and mTOR at −10.24 kcal/mol. The adoption of exosome/liposome-mediated delivery significantly amplified the bioavailability and cellular uptake of these nano-formulated compounds, positioning them as potential stalwarts in ovarian cancer intervention. A brief explanation of exosome/liposome-mediated delivery involves the use of these vesicles to encapsulate and transport therapeutic agents directly to the target cells, enhancing drug delivery efficiency and minimizing side effects. Conclusion: Addressing ovarian cancer’s intricacies, dominated by the erratic PI3K/Akt/mTOR signaling, mandates innovative therapeutic strategies. Our pioneering approach converges nanotechnological liposomal delivery with mangiferin and curcumin’s natural efficacies. This confluence, validated by computational insights, heralds a paradigm shift in ovarian cancer treatment. As our findings underscore the collaborative potential of these phytochemicals, it beckons further exploration in translational studies and clinical applications, ensuring the best intersection of nature and technology for therapeutic advantage.
Bias Correction of Humidty Measurements by Radio Sondes of Vaisala RS 92

Moisture is very important for many atmospheric processes. A correct description of the moisture of NWP models is essential to simulate the hydrological cycle precisely. An appropriate way to get the model a realistic moisture field is to assimilate moisture observations. In all operational configurations of the COSMO model, radio sondes are the only source of observational information on humidity, except for sceen-level data, which are given very limited weight however. Therefore any systematic error in the radio sondes humidity data will likely be detrimental. Recently many investigations revealed that the humidity measurements of radio sondes seems to be biased compared to other humidity observations. In the two operational configurations of Deutscher Wetterdienst (DWD), COSMO-EU, which has a mesh width of 7 km and covers Europe, and COSMO-DE (2.8 km, Germany and environs) 56% resp. 81% of all radio sondes used are of type Vaisala RS 92. Miloshevich et al. (2009) investigated the accuracy of this type of radio sondes as a function of height, solar elevation angle and relative humidity. They mention two different reasons for the bias. First, there is a calibration error which leads to a small time-independent moist bias below 500 hPa and dry bias further above. The second error is caused by a solar radiation error and will lead to a significant dry bias during daytime. Keeping in mind that the radio sondes are mainly launched at about 00 and 12 UTC the dry bias will affect the COSMO models at daytime between 9 and 12 UTC. To reveal the influence on the COSMO models integrated water vapour (IWV) of the model analyses is compared to the independent IWV retrievals of GNSS zenith total delay measurements (Gendt et al., 2004). As shown in fig. , the model tends to be moister at night but is much drier about noon. A correction algorithm after Milosevisch et al. is now implemented within the COSMO code and briefly described in the following section. Section 3 presents the investigations of the application of the bias correction. A conclusion is drawn in section 4.
Species composition and succession in yellow pine stands following southern pine beetle outbreaks in Tennessee-preliminary results

The southern pine beetle (Dendroctonus frontalis) is a bark beetle that is native to the Southern United States, including Tennessee. The beetle is periodically epidemic and can cause high levels of mortalityduring epidemic years, particularly in dense or aging pine (Pinus spp.) stands. An epidemic outbreak of the Southern pine beetle occurred in 1999-2001. By 2001, at the peak of the epidemic, 55 counties in Tennessee were in outbreak status. Subsequent estimations suggest that over 350,000 acres of pine timber in the State were affected by the outbreak, causing hundreds of millions of dollars in damage. Given the relative scarcity of the softwood resource in the State compared to the abundance of hardwood species and the significant economic importance of softwoods in Tennessee, the composition and successional trajectory of pine stands impacted by southern pine beetle in the most recent 1999-2001 outbreak is of interest. Here, we measure and quantify the impacts of this southern pine beetle outbreak on the successional trajectory of impacted yellow pine stands. Plots from the Forest Service, U.S. Department of Agriculture Forest Inventory and AnalysisProgram measured prior, during, and after the outbreak are used to estimate the changes that occurred in southern yellow pine systems within Tennessee. The results from this study suggest that approximately 25 percent of the softwood-dominated forests in Tennessee was lost following the 2000 southern pine beetle event. The majority of that lost acreage transitioned into hardwood-dominated communities.
Exploring the impact of managerial abilities on the competitive advantage and performance of creative industries in Indonesia during the post-pandemic recovery phase

In the pre-COVID-19 era, the creative fashion industry stood as a robust pillar in Indonesia's economy, contributing to over 50 percent of the nation's creative economy exports. Batik clothing and hijabs were at the forefront, emerging as the key export products. However, with the advent of the COVID-19 pandemic, this once thriving sector suffered substantial setbacks. This study aims to investigate how managerial ability influences competitive advantage, and whether the performance of that small-medium creative sectors is directly affected by managerial ability or indirectly through the mediation of competitive advantage. Our study aims to extent the existing studies by focusing on the sustained resilience of businesses in a post-pandemic. The study's sample consists of 134 Batik business owners and/or managers from East Java, Indonesia. The gathered data was then processed and analyzed using a structural equation model. Although the findings indicated that managerial ability did not directly affect business performance, there was a positive relationship between managerial ability and business performance when mediated by the competitive advantage. These findings suggest that competitive advantage continues to play a crucial role in business performance. The higher the managerial ability, the greater the potential to enhance their competitive, which in turn escalates their performance.
Effect of Watering Interval Cropping Pattern on The Performance Of Pearl Millet (Pennisetum Glaucum (L)

One field experiment was conducted for two consecutive seasons (2005/06 and 2006/07) in the Demonstration Farm of the Faculty of Agriculture, University of Khartoum at Shambat. The objective of the study is to determine the effect of watering interval and cropping pattern on growth and yield of pearl millet (Pennisetum glaucum L.). From the total productivity, Land Equivalent Ratio (LER) of pearl millet mixed with cowpea (Vigna unguiculata L. Walp.) was also calculated. In this study a local cultivar of pearl millet (Dimbi) and cowpea (Lubia) were sown under three watering intervals and two cropping patterns. With respect to watering treatments the plants were irrigated every one week (W1), two weeks (W2) and three weeks (W3). The cropping patterns were either sowing millet as sole crop (MS) or in mixture (MM) with cowpea. The experimental treatments were arranged in split-plot design with four replications in both seasons. The watering treatments were assigned to the main plots and the cropping patterns to the sub-plots. Characters studied include growth attributes (plant height, number of tillers/plant, number of leaves/plant, leaf area/plant, number of nodes, internodes length, shoot fresh weight, shoot dry weight, root fresh weight and root dry weight), phonological attributes (number of days to start of flowering, number of days to 50% flowering, and number of days to physiological maturity) and reproductive attributes (head length, head weight, number of heads/plant, number of seeds/head, 1000 seed weight and seed yield).
Balance Training and Shooting Performance: The Role of Load and the Unstable Surface

Military and law enforcement members’ shooting ability is influenced by their postural balance, which affects their performance and survivability. This study aimed to investigate the effects of a proprioception training program (standing or walking on unstable surfaces) on postural balance and shooting performance. Twenty participants, divided into two groups, completed 60 shots in a shooting simulator while standing, before and after a 4-week proprioception training program. One group (n = 10) followed the training program (EXP), while the other group followed the regular military academy program (CON). The shooting was conducted under four conditions: without load on a stable surface, with load on a stable surface, without load on an unstable surface, and with load on an unstable surface. The findings reveal that the training program had a significant impact on the EXP, improving their balance (p < 0.01). Additionally, only in the EXP, shooting score and the percentage center of gravity increased (p < 0.01) and the stability of the shots, measured by holding time on the target, doubled from 2.2 to 4.5 s (p < 0.01). These improvements were more pronounced when participants had a load and/or were on an unstable surface. In conclusion, a proprioception training program could be beneficial for improving postural balance and shooting performance.
TOWARDS A GENERIC ONTOLOGY FOR SOLAR IRRADIANCE FORECASTING

The growth of solar energy resources in recent years has led to increased calls for accurate forecasts of solar irradiance for the reliable and sustainable integration of solar into the national grid. A growing body of academic research has developed models for forecasting solar irradiance, identified metrics for comparing solar forecasts, and described applications and end users of solar forecasts. In recent years, many disciplines are developing ontologies to facilitate better communication, improve inter-operabiity and refine knowledge reuse by experts and users of the domain. Ontologies are explicit and formal vocabulary of terms and their relationships. This report describes a step towards using ontologies to describe the knowledge, concepts and relationships in the domain of solar irradiance forecasting to develop a shared understanding for diverse stakeholders that interact with the domain. A preliminary ontology on solar irradiance forecasting was created and validated on three use cases.
Multi-omics reveals changed energy metabolism of liver and muscle by caffeine after mice swimming

In recent years, numerous studies have investigated the effects of caffeine on exercise, and provide convincing evidence for its ergogenic effects on exercise performance. However, the precise mechanisms underlying these ergogenic effects remain unclear. In this study, an exercise swimming model was conducted to investigate the effects of orally administered with caffeine before swimming on the alterations of proteome and energy metabolome of liver and muscle after swimming. We found proteins in liver, such as S100a8, S100a9, Gabpa, Igfbp1 and Sdc4, were significantly up-regulated, while Rbp4 and Tf decreased after swimming were further down-regulated in caffeine group. The glycolysis and pentose phosphate pathways in liver and muscle were both significantly down-regulated in caffeine group. The pyruvate carboxylase and amino acid levels in liver, including cysteine, serine and tyrosine, were markedly up-regulated in caffeine group, exhibiting a strong correlation with the increased pyruvic acid and oxaloacetate levels in muscle. Moreover, caffeine significantly decreased the lactate levels in both liver and muscle after swimming, potentially benefiting exercise performance.
The LINC01176-miR-218-5p-IL-36G Network is Responsible for the Pathogenesis of Psoriasis by Promoting Inflammation

Purpose Psoriasis is an incurable chronic inflammatory skin disease. The exact function and regulatory mechanism of non-coding RNA upregulation in psoriasis remains to be elucidated. The aim of this study was to analyse the role of the lncRNA-miRNA-mRNA network of psoriasis and LINC01176 in the pathogenesis of psoriasis. Patients and Methods We performed miRNA, lncRNA, and mRNA sequencing analysis in pretreatment and treatment psoriatic tissues and normal tissues, constructed an lncRNA-miRNA-mRNA coexpression network and screened mRNA-associated pathways using bioinformatics analysis. We further validated the regulatory role of LINC01176-miR-218-5p on the proliferation and inflammation of the psoriatic model by dual-luciferase reporter assay, cell transfection, CCK-8 method, TUNEL staining and animal model construction method. An lncRNA-miRNA-mRNA coexpression network was successfully constructed by RNA-seq data analysis. Results We obtained the relationship between LINC01176, miR-218-5p and IL36-G. Analysis of the apoptotic and proliferative capacity of the transfected cells showed that miR-218-5p up-regulation significantly inhibited cell proliferation and promoted apoptosis. A mouse model of psoriasis was successfully established. Phenotypic observations revealed that keratin-forming cells in mice coated with LINC01176-shRNA emulsifier were significantly lower than those in the model group and close to those in the normal group. HE and immunohistochemical experiments were performed, and the results showed the role and mechanism of action of LINC01176-shRNA. Suppression of LINC01176 significantly inhibited the expression of IL-36G in psoriatic tissues. LINC01176 showed a targeting and positive correlation with IL36-G expression. Conclusion Our study shows that LINC01176 promotes the proliferation and invasion of keratinocytes and inhibits apoptosis by targeting miR-218-5p, which acts as a repressor of the psoriasis-associated IL-36G. The shRNA-LINC01176 emulsion showed potential treatment capability in alleviating symptoms of psoriasis.
Simultaneous Quantification of Acetaminophen, Caffeine, and Ibuprofen in Fixed Dose Combination Drug Using HPLC with UV Detection

Combination therapy of analgesics is well suited for pain management especially in elderly patients and, has been recommended by the World Health Organization (WHO). Drug analysis plays an important role in the development, manufacture and therapeutic use of drug. In this study, a suitable, cost effective Isocratic HPLC-UV method (Reversed Phase) has been developed and validated for the simultaneous quantification of Acetaminophen, Caffeine, and Ibuprofen in fixed dose combination drugs, using a mobile phase combination of methanol and 0.025M Phosphate buffer –(adjusted to pH 3.2 with Orthophosphoric acid) in the ratio 85:15.A Vertex Column-Eurospher C18 (250 x 4.6 mm), flow rate of 1.0 ml/min at 25°C were the chromatographic conditions. With Piroxicam as internal standard, quantification was achieved with UV detection at 225 nm based on the peak area responses. A good resolution and a short run time (7mins) were achieved with the validated conditions. In consequence, statistical evaluation at the 95% confidence limits revealed that, the method was Linear; with an average correlation coefficient (R = 0.995), and accurate - (mean recovery 99.45% for Acetaminophen, 100.10% for Caffeine and 99.28% for Ibuprofen). With an instrument and intermediate precision RSDs>2.0, the method was found to be specific, Robust, and more economical. Six formulated combination products on the Ghana market were assayed using the validated method. The Acetaminophen, Caffeine and Ibuprofen contents in the combination drugs varied from 97.35% to 103.88%.