Miracle-dz/UniFAR-FTUs huggingface dataset:
MicroRNA 23b regulates autophagy associated with radioresistance of pancreatic cancer cells.

BACKGROUND & AIMS Tumor resistance to radiation is a challenge in the treatment of patients with pancreatic cancer. Improving our understanding of the mechanisms of radioresistance could lead to strategies to increase patients' response to therapy. We investigated the roles of microRNAs (miRNAs) involved in radioresistance of pancreatic cancer cells. METHODS We established radioresistant pancreatic cancer cell lines and used array analysis to compare levels of different miRNAs between radioresistant cell lines and the parental cell lines from which they were derived. We transfected pancreatic cancer cells with miRNA mimics or inhibitors and evaluated their effects on cell radiosensitivity using a clonogenic survival assay. The effects of miRNA on autophagy were determined by transmission electron microscopy and immunoblot analysis. We used a luciferase reporter assay to identify messenger RNA targets of specific miRNAs. RESULTS Radioresistant pancreatic cancer cells had reduced levels of the miRNA miR-23b and increased autophagy compared with cells that were not radioresistant. Overexpression of miR-23b inhibited radiation-induced autophagy, whereas an inhibitor of miR-23b promoted autophagy in pancreatic cancer cells. Overexpression of miR-23b sensitized pancreatic cancer cells to radiation. The target of miR-23b, ATG12, was overexpressed in radioresistant cells; levels of ATG12 protein correlated with the occurrence of autophagy. Expression of miR-23b blocked radiation-induced autophagy and sensitized pancreatic cancer cells to radiation. We observed an inverse correlation between the level of miR-23b and autophagy in human pancreatic cancer tissue samples. CONCLUSIONS In pancreatic cancer cells, reduced levels of the miRNA miR-23b increase levels of ATG12 and autophagy to promote radioresistance. miR-23b might be used to increase the sensitivity of pancreatic cancer cells to radiation therapy.
Trauma and Couple Therapy

Abstract While the most common reaction to trauma is resilience, trauma can significantly alter one's ability to attach to another. Post traumatic stress disorder and other traumatic responses can have significant effects on couple relationships. In this chapter, the effect of trauma on couples is explored. Of particular significance to clinicians is the effect of childhood maltreatment on adult attachment bonds. As such, the relational ramifications of childhood maltreatment on couple relationships is discussed. While the potential damage trauma can inflict upon a couple relationship is remarkable, the source of healing that a couple relationship lends is also powerful. Relationship attachments appear to be a central mechanism in healing from trauma. As such, treating trauma within the context of the couple relationship leaves therapists with a particularly potent tool with which to address healing the wounds of past and present trauma. Couple therapy that addresses trauma can also allow the couple to assume a new, more cohesive whole.
Molecular Dissection of Formation of Senescence-Associated Heterochromatin Foci

ABSTRACT Senescence is characterized by an irreversible cell proliferation arrest. Specialized domains of facultative heterochromatin, called senescence-associated heterochromatin foci (SAHF), are thought to contribute to the irreversible cell cycle exit in many senescent cells by repressing the expression of proliferation-promoting genes such as cyclin A. SAHF contain known heterochromatin-forming proteins, such as heterochromatin protein 1 (HP1) and the histone H2A variant macroH2A, and other specialized chromatin proteins, such as HMGA proteins. Previously, we showed that a complex of histone chaperones, histone repressor A (HIRA) and antisilencing function 1a (ASF1a), plays a key role in the formation of SAHF. Here we have further dissected the series of events that contribute to SAHF formation. We show that each chromosome condenses into a single SAHF focus. Chromosome condensation depends on the ability of ASF1a to physically interact with its deposition substrate, histone H3, in addition to its cochaperone, HIRA. In cells entering senescence, HP1γ, but not the related proteins HP1α and HP1β, becomes phosphorylated on serine 93. This phosphorylation is required for efficient incorporation of HP1γ into SAHF. Remarkably, however, a dramatic reduction in the amount of chromatin-bound HP1 proteins does not detectably affect chromosome condensation into SAHF. Moreover, abundant HP1 proteins are not required for the accumulation in SAHF of histone H3 methylated on lysine 9, the recruitment of macroH2A proteins, nor other hallmarks of senescence, such as the expression of senescence-associated β-galactosidase activity and senescence-associated cell cycle exit. Based on our results, we propose a stepwise model for the formation of SAHF.
Identification of the Transmembrane Glucose Regulated Protein 78 as a Biomarker for the Brain Cancer Glioblastoma Multiforme by Gene Expression and Proteomic Studies

The prognosis of patients with Glioblastoma Multiforme (GBM), the most malignant adult glial brain tumor, remains poor in spite of advances in treatment procedures, including surgical resection, irradiation and chemotherapy. Genetic heterogeneity of GBM warrants extensive studies to gain a thorough understanding of the biology of this tumor. While there have been several studies of global transcript profiling of glioma with the identification of gene signatures for diagnosis and disease management, translation into clinics is yet to happen. In the present study, we report a novel proteomic approach by using two-dimensional difference gel electrophoresis (2D-DIGE) followed by spot picking and analysis of proteins/peptides by Mass Spectrometry. We report Glucose Regulated Protein 78 (GRP78) as a differentially expressed protein in the GBM cell line compared to human normal Astrocyte cells. In addition to proteomic studies, we performed microarray analysis which further confirmed up regulation of GRP78 in GBM cells compared to human normal Astrocyte cells. GRP78 has long been recognized as a molecular chaperone in the endoplasmic reticulum (ER) and can be induced by the ER stress response. Besides its location in the ER, GRP78 has been found in cell plasma membrane, cytoplasm, mitochondria, nucleus and other cellular secretions. GRP78 is implicated in tumor cell proliferation, apoptosis resistance, immune escape, metastasis and angiogenesis, and its elevated expression usually correlates with a variety of tumor micro environmental stresses, including hypoxia, glucose deprivation, lactic acidosis and inflammatory response. GRP78 protein acts as a centrally located sensor of stress, which senses and facilitates the adaptation to the tumor microenvironment. Our findings showed differential expression of this gene in brain cancer GBM and thus confirm similarities in findings in existing transcriptional and translational studies. Thus, these findings could be of further importance for diagnostic, therapeutic and prognostic approaches for dealing with this highly malignant cancer.
Countering the new media: The resurgence of share maintenance in primetime network television

Despite recent interest in the new technologies, media researchers have only begun to examine the impact of remote control devices, VCRs, and cable television on inheritance effects. In this study, share maintenance in network primetime programming was found to have decreased in the 1983, 1984, and 1985 seasons. This was predicted from the rapid growth in cable television, VCR, and remote tuner penetration during this time. But, surprisingly, these decreases were reversed between 1986–1988. These results are discussed in the context of the changing composition of the network primetime television audience and possible adjustments in network promotion and scheduling practices.
ELCO3: Entity Linking with Corpus Coherence Combining Open Source Annotators

Entity Linking (EL) systems' performance is uneven across corpora or depending on entity types. To help overcome this issue, we propose an EL workflow that combines the outputs of several open source EL systems, and selects annotations via weighted voting. The results are displayed on a UI that allows the users to navigate the corpus and to evaluate annotation quality based on several metrics.
In vivo spatial frequency domain spectroscopy of two layer media

Abstract. Monitoring of tissue blood volume and local oxygen saturation can inform the assessment of tissue health, healing, and dysfunction. These quantities can be estimated from the contribution of oxyhemoglobin and deoxyhemoglobin to the absorption spectrum of the dermis. However, estimation of blood related absorption in skin can be confounded by the strong absorption of melanin in the epidermis and epidermal thickness and pigmentation varies with anatomic location, race, gender, and degree of disease progression. Therefore, a method is desired that decouples the effect of melanin absorption in the epidermis from blood absorption in the dermis for a large range of skin types and thicknesses. A previously developed inverse method based on a neural network forward model was applied to simulated spatial frequency domain reflectance of skin for multiple wavelengths in the near infrared. It is demonstrated that the optical thickness of the epidermis and absorption and reduced scattering coefficients of the dermis can be determined independently and with minimal coupling. Then, the same inverse method was applied to reflectance measurements from a tissue simulating phantom and in vivo human skin. Oxygen saturation and total hemoglobin concentrations were estimated from the volar forearms of weakly and strongly pigmented subjects using a standard homogeneous model and the present two layer model.
Meniere’s Disease and Vestibular Migraine: Updates and Review of the Literature

The diagnosis of Meniere’s disease (MD) and vestibular migraine (VM) is primarily based on clinical criteria and their differentiation is often difficult. Currently, there are no known definitive diagnostic tests that can reliably distinguish the two conditions. Patients with MD and patients with VM are treated differently, therefore improving the diagnosis of these two pathologies should avoid errors in management. A systematic review was conducted according to PRISMA guidelines. Medline-Ovid and Embase databases were used to conduct a thorough search of English-language publications dating from 1948 to March 2016. The primary search objective was to identify all papers explicitly comparing MD and VM in order to clarify and validate the diagnosis of these two diseases. A total of 13 articles out of 831 were reviewed. Among other differences, MD showed later age of onset, more hearing loss, tinnitus, aural fullness, abnormal nystagmus, abnormal caloric testing results, abnormal vestibular evoked myogenic potential and endolymphatic hydrops. VM showed more headaches, photophobia, vomiting and aura. Even though differences were noted between the two diseases, only one study focused on assessing the differences between VM, MD and patients fulfilling both diagnostic criteria (MDVM). This study showed no difference between the three groups. Since the introduction of the new International Headache Society and Barany Society criteria for VM, no studies have focused on comparing these three groups. We strongly encourage authors to focus on comparing MD and VM from MDVM in future studies to help adequately distinguish the diagnosis of both diseases.
Reporting Sexual Victimization To The Police And Others

Beginning with Koss, Gidycz, and Wisniewski’s pathbreaking study, the sexual victimization of female college students has emerged as salient research and policy concern. Building on this earlier work, we used a national, random sample of 4,446 female college students to focus on an issue of continuing importance: the level and determinants of victims’ willingness to report their sexual victimization. The analysis revealed that although few incidents—including rapes—are reported to the police and/or to campus authorities, a high proportion are disclosed to someone else (mainly to friends). Incidents were more likely to be reported to the police when they had characteristics that made them more “believable” (e.g., presence of a weapon or assailant who was a stranger). The use of alcohol and/or drugs by offenders and/or victims had a unique effect, causing students to be more likely to disclose their victimization to friends but not to campus authorities. The implications of the findings for extant debates and for future research are also explored.
The Processing of Positive and Negative Information

An affirmative statement which is known to be false and the complementary negative statement which is known to be true, provide the same information, i.e. that something is not the case. Similarly, an affirmative statement which is known to be true and the complementary negative statement which is known to be false, both imply that something is the case. (If P is false, not-P is true and if P is true, not-P is false.) Hence there are four kinds of statement (“conditions”): true affirmatives, false affirmatives, true negativee and false negatives, but only two kinds of information: positive and negative. This experiment investigates the times taken to process information presented in these ways. The task was to select two alternative words which would make affirmative or negative conjunctive statements agree or conflict with given situations. The four conditions were presented six times in different serial orders, so that each occurred once in every block of four trials. The mean response times were: true affirmatives 8–99 sec, false affirmatives 11 19 sec. , true negatives 12–58 sec, false negatives 15–17 sec. This order was the same at each of the six presentations of the conditions, the differences being significant at the 0 001 level in each case. There was a pronounced decline in errors (without knowledge of results) for three of the conditions. These results are discussed in relation to (i) the assumption of a positive set, established through a long learning process; (ii) the inferential nature of negative information in relation to experience; and (iii) the possible emotional effects of negative terms.
Cardiac autonomic modulation during recovery from acute endurance versus resistance exercise.

BACKGROUND The kinetics of parasympathetic reactivation after dynamic endurance exercise have been well elucidated. However, autonomic recovery of cardiac function after resistance exercise is not known. PURPOSE The purpose of this study was to assess cardiac autonomic modulations during recovery from acute resistance exercise versus acute endurance exercise. METHODS Electrocardiogram readings were collected before and 30 min after a single bout of endurance or resistance exercise in 14 male participants (aged 25.3+/-2.5 years). Heart rate (HR) variability was spectrally decomposed using an autoregressive approach. High frequency (HF) power was considered representative of vagal modulation. All values were expressed in both absolute and normalized units (normalized for change in total power). RESULTS A mode-by-time interaction (P<0.05) was detected for HR, which remained elevated to a greater extent after resistance exercise. Total power was significantly reduced after resistance exercise (P<0.05) but not endurance exercise. An interaction (P<0.05) was also detected for both a change in absolute natural log function HF power and natural log function low frequency (LF) power (P<0.05), as both variables decreased more after resistance exercise. When normalized for changes in total power, interactions in LF and HF power were lost. The LF/HF ratio was significantly increased after both resistance and endurance exercise (P<0.05). CONCLUSION Greater elevations in HR after acute resistance exercise versus acute endurance exercise may be related to greater reductions in cardiac parasympathetic tone.
A Substantive Process Analysis of Responses to Items from the Multistate Bar Examination

We investigated examinees' cognitive processes while they solved selected items from the Multistate Bar Exam (MBE), a high-stakes professional certification examination. We focused on ascertaining those mental processes most frequently used by examinees, and the most common types of errors in their thinking. We compared the relationships between different problem-solving processes, errors, and performance, and assessed the degree to which performance was related to correspondence between examinees' and model problem-solving approaches. Participants most commonly solved problems with reference to legal principles and cues presented in problem stems, but also resorted to other mental processes. We found that performance on the MBE is strongly related to domain-specific skill in using legal principles, as well as to domain-general metacognitive skill in organizing thinking. Evidence that would suggest that testwiseness strategies or common-sense reasoning was a source of construct-irrelevant variance on the MBE was not found. Our study demonstrated a systematic method for in-depth analysis of examinee substantive processes on examinations such as the MBE that assess complex reasoning within a specific domain.
Intestinal Stem Cell Markers in the Intestinal Metaplasia of Stomach and Barrett’s Esophagus

Gastric intestinal metaplasia (IM) is a highly prevalent preneoplastic lesion; however, the molecular mechanisms regulating its development remain unclear. We have previously shown that a population of cells expressing the intestinal stem cell (ISC) marker LGR5 increases remarkably in IM. In this study, we further investigated the molecular characteristics of these LGR5 + cells in IM by examining the expression profile of several ISC markers. Notably, we found that ISC markers—including OLFM4 and EPHB2—are positively associated with the CDX2 expression in non-tumorous gastric tissues. This finding was confirmed in stomach lesions with or without metaplasia, which demonstrated that OLFM4 and EPHB2 expression gradually increased with metaplastic progression. Moreover, RNA in situ hybridization revealed that LGR5 + cells coexpress several ISC markers and remained confined to the base of metaplastic glands, reminiscent to that of normal intestinal crypts, whereas those in normal antral glands expressed none of these markers. Furthermore, a large number of ISC marker-expressing cells were diffusely distributed in gastric adenomas, suggesting that these markers may facilitate gastric tumorigenesis. In addition, Barrett’s esophagus (BE)—which is histologically similar to intestinal metaplasia—exhibited a similar distribution of ISC markers, indicating the presence of a stem cell population with intestinal differentiation potential. In conclusion, we identified that LGR5 + cells in gastric IM and BE coexpress ISC markers, and exhibit the same expression profile as those found in normal intestinal crypts. Taken together, these results implicate an intestinal-like stem cell population in the pathogenesis of IM, and provide an important basis for understanding the development and maintenance of this disease.
Simulating rigid body fracture with surface meshes

We present a new brittle fracture simulation method based on a boundary integral formulation of elasticity and recent explicit surface mesh evolution algorithms. Unlike prior physically-based simulations in graphics, this avoids the need for volumetric sampling and calculations, which aren't reflected in the rendered output. We represent each quasi-rigid body by a closed triangle mesh of its boundary, on which we solve quasi-static linear elasticity via boundary integrals in response to boundary conditions and loads such as impact forces and gravity. A fracture condition based on maximum tensile stress is subsequently evaluated at mesh vertices, while crack initiation and propagation are formulated as an interface tracking procedure in material space. Existing explicit mesh tracking methods are modified to support evolving cracks directly in the triangle mesh representation, giving highly detailed fractures with sharp features, independent of any volumetric sampling (unlike tetrahedral mesh or level set approaches); the triangle mesh representation also allows simple integration into rigid body engines. We also give details on our well-conditioned integral equation treatment solved with a kernel-independent Fast Multipole Method for linear time summation. Various brittle fracture scenarios demonstrate the efficacy and robustness of our new method.
The Structural Reform of Secondary Education in China.

In the 1980s, the Chinese government undertook a major structural reform in education by which upper secondary education was converted from predominantly general education to an equal mix of general education and vocational/technical education. A critical examination is provided of the rationale for and implementation strategies of the reform, framed in a broader context of the development of secondary education in the past four decades. It points out that, although the reform was justified in largely economic terms, there is actually little empirical support for the economic assumptions; the development of vocational/technical education is prompted more by a desire to reduce the social demand for higher education and to use education as a social stratification device. The reform reflects changing perspectives of the Chinese leadership on the role of education in national development; and it can be seen as the outcome of the most recent episode of continuing social and political conflicts in the Chinese s.
The economics of linguistic exchanges

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Cognitive and brain magnetic resonance imaging findings in adrenomyeloneuropathy

Neuropsychological functioning and brain magnetic resonance imaging (MRI) were evaluated in 84 men with adrenomyeloneuropathy (AMN). MRI was normal in 61%, the “pure AMN” group, while 39%, the “cerebral AMN” group, showed brain white matter abnormalities. Except for mild deficits in psychomotor speed and visual memory, neuropsychological function was normal in pure AMN. Most patients with cerebral AMN had normal IQ and language but evidenced impaired psychomotor speed, spatial cognition, memory, and executive functions. Patients with MRI evidence of very severe cerebral disease had global and language impairment as well, and deficits in all patients were highly correlated with degree of brain MRI involvement.