Eating and Weight Disorders. Studies on Anorexia, Bulimia and Obesity

: Purpose: Polycystic ovary syndrome (PCOS) is a common cause of endocrine disorder and infertility among women in which is related with low grade inflammation. Therefore, this current randomized, double-blind, placebo-controlled clinical trial assessed the effects of green cardamom supplementation on inflammatory markers and gene expression among obese women with PCOS. Methods: We included 194 obese PCOS women that gave all of them low calorie diet. These subjects were randomly divided in two studied groups including intervention with 3 g/day green cardamom (n=99) and placebo groups (n=95). Anthropometric indices, androgen hormones, and inflammatory factors (tumor necrosis factor-a (TNF-α), Interleukin 6 (IL-6), and C- reactive protein (CRP)) were assessed before and after four months intervention. Their TNF-α, IL-6, and CRP genes expression level were measured using reverse transcription-polymerase chain reaction (RT-PCR) method. Results: Anthropometric indices were improved in both two studied groups (P<0.001). Among androgen hormones luteinizing hormone, androstenedione, and dehydroepiandrosterone were significantly decreased (P<0.001), as well as, follicle-stimulating hormone was significantly increased (P<0.001) in the green cardamom group. Our findings showed that TNF-α, IL-6, and CRP serum were significantly decreased after the intervention with green cardamom plus low calorie diet (P<0.001). In addition, the expression level of TNF-α and CRP genes were significantly decreased in intervention groups (P<0.001). Conclusions: This present study support the beneficial anti-inflammatory effect of green cardamom on the inflammatory status in the PCOS women. Abstract Purpose: Polycystic ovary syndrome (PCOS) is a common cause of endocrine disorder and infertility among women in which is related with low grade inflammation. Therefore, this current randomized, double-blind, placebo-controlled clinical trial assessed the effects of green cardamom supplementation on inflammatory markers and gene expression among obese women with PCOS. Methods : We included 194 obese PCOS women that gave all of them low calorie diet. These subjects were randomly divided in two studied groups including intervention with 3 g/day green cardamom (n=99) and placebo groups (n=95). Anthropometric indices, androgen hormones, and inflammatory factors (tumor necrosis factor-a (TNF-α), Interleukin 6 (IL-6), and C- reactive protein (CRP)) were assessed before and after four months intervention. Their TNF-α, IL-6, and CRP genes expression level were measured using reverse transcription-polymerase chain reaction (RT-PCR) method. Results : Anthropometric indices were improved in both two studied groups (P<0.001). Among androgen hormones luteinizing hormone, androstenedione, and dehydroepiandrosterone were significantly decreased (P<0.001), as well as, follicle-stimulating hormone was significantly increased (P<0.001) in the green cardamom group. Our findings showed that TNF-α, IL-6, and CRP serum were significantly decreased after the intervention with green cardamom plus low calorie diet (P<0.001). In addition, the expression level of TNF-α and CRP genes were significantly decreased in intervention groups (P<0.001). Conclusions : This present study support the beneficial anti-inflammatory effect of green cardamom on the inflammatory status in the PCOS women. of the green cardamom on the inflammatory factors and genes expression among obese PCOS women with low calorie diet.