Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of the Male Ejaculate on Post-Mating Prezygotic Barriers in Field Crickets

Post-copulatory interactions between males and females involve highly coordinated, complex traits that are often rapidly evolving and divergent between species. Failure to produce and deposit eggs may be a common post-mating prezygotic barrier, yet little is known about what prevents the induction of egg-laying between species. The field crickets, Gryllus firmus and G. pennsylvanicus are isolated by a one-way reproductive incompatibility; G. pennsylvanicus males fail to fertilize G. firmus eggs or to induce normal egg-laying in G. firmus females. We use experimental crosses to elucidate the role of accessory gland-derived vs. testis-derived components of the G. firmus male ejaculate on egg-laying in conspecific and heterospecific crosses. Using surgical castrations to create ‘spermless’ males that transfer only seminal fluid proteins (SFPs) we test whether G. firmus male SFPs can induce egg-laying in conspecific crosses and rescue egg-laying in crosses between G. pennsylvanicus males and G. firmus females. We find G. firmus SFPs induce only a small short-term egg-laying response and that SFPs alone cannot explain the normal induction of egg-laying. Gryllus firmus SFPs also do not rescue the heterospecific cross. Testis-derived components, such as sperm or prostaglandins, most likely stimulate egg-laying or act as transporters for SFPs to targets in the female reproductive tract. These results highlight the utility of experimental approaches for investigating the phenotypes that act as barriers between species and suggest that future work on the molecular basis of the one-way incompatibility between G. firmus and G. pennsylvanicus should focus on divergent testis-derived compounds or proteins in addition to SFPs.
The mycorrhiza-dependent defensin MtDefMd1 of Medicago truncatula acts during the late restructuring stages of arbuscule-containing cells

Different symbiotic and pathogenic plant-microbe interactions involve the production of cysteine-rich antimicrobial defensins. In Medicago truncatula, the expression of four MtDefMd genes, encoding arbuscular mycorrhiza-dependent defensins containing an N-terminal signal peptide and exhibiting some differences to non-symbiotic defensins, raised over the time of fungal colonization. Whereas the MtDefMd1 and MtDefMd2 promoters were inactive in cells containing young arbuscules, cells with fully developed arbuscules displayed different levels of promoter activities, indicating an up-regulation towards later stages of arbuscule formation. MtDefMd1 and MtDefMd2 expression was absent or strongly down-regulated in mycorrhized ram1-1 and pt4-2 mutants, known for defects in arbuscule branching or premature arbuscule degeneration, respectively. A ~97% knock-down of MtDefMd1/MtDefMd2 expression did not significantly affect arbuscule size. Although overexpression of MtDefMd1 in arbuscule-containing cells led to an up-regulation of MtRam1, encoding a key transcriptional regulator of arbuscule formation, no morphological changes were evident. Co-localization of an MtDefMd1-mGFP6 fusion with additional, subcellular markers revealed that this defensin is associated with arbuscules in later stages of their life-cycle. MtDefMd1-mGFP6 was detected in cells with older arbuscules about to collapse, and ultimately in vacuolar compartments. Comparisons with mycorrhized roots expressing a tonoplast marker indicated that MtDefMd1 acts during late restructuring processes of arbuscule-containing cells, upon their transition into a post-symbiotic state.
Creating Band Gaps in Periodic Media

We identify explicit conditions on geometry and material contrast for creating band gaps in two-dimensional photonic and three-dimensional acoustic crystals. This approach is novel and makes use of the electrostatic and quasi-periodic source free resonances of the crystal. The source free modes deliver a spectral representation for solution operators associated with propagation of electromagnetic and acoustic waves inside periodic high contrast media. An accurate characterization of the quasi-periodic and electrostatic resonance spectrum in terms of the shape and geometry of the scatters is possible. This information together with the Dirichlet and a Neumann-like spectra associated with the inclusions deliver conditions sufficient for opening band gaps at finite contrast. The theory provides a systematic means for the identification of photonic and phononic band gaps within a specified frequency range.
Low-Power, Real-Time Object-Recognition Processors for Mobile Vision Systems

A new low-power object-recognition processor achieves real-time robust recognition, satisfying modern mobile vision systems' requirements. The authors introduce an attention-based object-recognition algorithm for energy efficiency, a heterogeneous multicore architecture for data- and thread-level parallelism, and a network on a chip for high on-chip bandwidth. The fabricated chip achieves 30 frames/second throughput and an average 320 mW power consumption on test 720p video sequences, yielding 640 GOPS/W and 10.5 NJ/pixel energy efficiency.
Social Psychology and Human-Robot Interaction: An Uneasy Marriage

The field of Human-Robot Interaction (HRI) lies at the intersection of several disciplines, and is rightfully perceived as a prime interface between engineering and the social sciences. In particular, our field entertains close ties with social and cognitive psychology, and there are many HRI studies which build upon commonly accepted results from psychology to explore the novel relation between humans and machines. Key to this endeavour is the trust we, as a field, put in the methodologies and results from psychology, and it is exactly this trust that is now being questioned across psychology and, by extension, should be questioned in HRI. The starting point of this paper are a number of failed attempts by the authors to replicate old and established results on social facilitation, which leads us to discuss our arguable over-reliance and over-acceptance of methods and results from psychology. We highlight the recent "replication crisis" in psychology, which directly impacts the HRI community and argue that our field should not shy away from developing its own reference tasks.
Understanding the prevalence and correlates of implicit theories of weight in the United States: Insights from a nationally representative sample

Objectives: The primary aim of this research is to understand how mindsets about weight controllability in the United States relate to population health. We examined the distribution of people’s implicit theories of weight, from an incremental (controllable) to an entity (not controllable) mindset, in a nationally representative sample, as well as their relation to: sociodemographic factors, beliefs about behaviour and genetics as causes of obesity and engagement in weight management-relevant behaviours. Methods: We report data from the National Cancer Institute’s Health Information National Trends Survey 4. Results: A majority of respondents endorsed an incremental mindset of body weight, but endorsement of this mindset was stronger among younger, white respondents, and those with a higher income and more educational attainment. A stronger incremental mindset was related to stronger behaviour and weaker genetic causal beliefs about obesity, as well as a tendency to report increased engagement in weight management-relevant behaviours. Conclusions: Our research provides evidence that although incremental mindsets are more common overall and associated with engagement in health behaviours that can contribute to or detract from population health, incremental mindsets are less common among individuals from more marginalised groups.
Approximating Generalized Network Design under (Dis)economies of Scale with Applications to Energy Efficiency

In a generalized network design (GND) problem, a set of resources are assigned (non-exclusively) to multiple requests. Each request contributes its weight to the resources it uses and the total load on a resource is then translated to the cost it incurs via a resource-specific cost function. Motivated by energy efficiency applications, recently, there is a growing interest in GND using cost functions that exhibit (dis)economies of scale ((D)oS), namely, cost functions that appear subadditive for small loads and superadditive for larger loads. The current article advances the existing literature on approximation algorithms for GND problems with (D)oS cost functions in various aspects: (1) while the existing results are restricted to routing requests in undirected graphs, identifying the resources with the graph’s edges, the current article presents a generic approximation framework that yields approximation results for a much wider family of requests (including various types of Steiner tree and Steiner forest requests) in both directed and undirected graphs, where the resources can be identified with either the edges or the vertices; (2) while the existing results assume that a request contributes the same weight to each resource it uses, our approximation framework allows for unrelated weights, thus providing the first non-trivial approximation for the problem of scheduling unrelated parallel machines with (D)oS cost functions; (3) while most of the existing approximation algorithms are based on convex programming, our approximation framework is fully combinatorial and runs in strongly polynomial time; (4) the family of (D)oS cost functions considered in the current article is more general than the one considered in the existing literature, providing a more accurate abstraction for practical energy conservation scenarios; and (5) we obtain the first approximation ratio for GND with (D)oS cost functions that depends only on the parameters of the resources’ technology and does not grow with the number of resources, the number of requests, or their weights. The design of our approximation framework relies heavily on Roughgarden’s smoothness toolbox [43], thus demonstrating the possible usefulness of this toolbox in the area of approximation algorithms.
Archaeal acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site

Significance Mevalonate is a building block of archaeal lipids. Three enzymes are involved in its biosynthesis: acetoacetyl-CoA thiolase (thiolase), 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase (HMGCS), and HMG-CoA reductase. The thiolase reaction is highly endergonic, which means that archaea have to find a way to overcome this low-flux bottleneck. Our work revealed the presence of a thiolase/HMGCS complex, which directly couples the endergonic thiolase reaction to the exergonic HMGCS reaction. An unexpected third protein spatially connects the thiolase and HMGCS. Strikingly, these two enzymes share the same substrate-binding site. Genomic information indicated that the presence of a thiolase/HMGCS complex is common in most of archaea and many bacteria. Such a natural intermediate-channeling system could lead to new strategies to improve biotechnological mevalonate synthesis. Many reactions within a cell are thermodynamically unfavorable. To efficiently run some of those endergonic reactions, nature evolved intermediate-channeling enzyme complexes, in which the products of the first endergonic reactions are immediately consumed by the second exergonic reactions. Based on this concept, we studied how archaea overcome the unfavorable first reaction of isoprenoid biosynthesis—the condensation of two molecules of acetyl-CoA to acetoacetyl-CoA catalyzed by acetoacetyl-CoA thiolases (thiolases). We natively isolated an enzyme complex comprising the thiolase and 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase (HMGCS) from a fast-growing methanogenic archaeon, Methanothermococcus thermolithotrophicus. HMGCS catalyzes the second reaction in the mevalonate pathway—the exergonic condensation of acetoacetyl-CoA and acetyl-CoA to HMG-CoA. The 380-kDa crystal structure revealed that both enzymes are held together by a third protein (DUF35) with so-far-unknown function. The active-site clefts of thiolase and HMGCS form a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction. The tripartite complex is found in almost all archaeal genomes and in some bacterial ones. In addition, the DUF35 proteins are also important for polyhydroxyalkanoate (PHA) biosynthesis, most probably by functioning as a scaffold protein that connects thiolase with 3-ketoacyl-CoA reductase. This natural and highly conserved enzyme complex offers great potential to improve isoprenoid and PHA biosynthesis in biotechnologically relevant organisms.
Less Rhyme, More Reason: Knowledge-based Poetry Generation with Feeling, Insight and Wit

Linguistic creativity is a marriage of form and content in which each works together to convey our meanings with concision, resonance and wit. Though form clearly influences and shapes our content, the most deft formal trickery cannot compensate for a lack of real insight. Before computers can be truly creative with language, we must first imbue them with the ability to formulate meanings that are worthy of creative expression. This is especially true of computer-generated poetry. If readers are to recognize a poetic turn-of-phrase as more than a superficial manipulation of words, they must perceive and connect with the meanings and the intent behind the words. So it is not enough for a computer to merely generate poem-shaped texts; poems must be driven by conceits that build an affective worldview. This paper describes a conceit-driven approach to computational poetry, in which metaphors and blends are generated for a given topic and affective slant. Subtle inferences drawn from these metaphors and blends can then drive the process of poetry generation. In the same vein, we consider the problem of generating witty insights from the banal truisms of common-sense knowledge bases.
Influence of PEG Stoichiometry on Structure-Tuned Formation of Self-Assembled Submicron Nickel Particles

Self-assembled submicron nickel particles were successfully synthesized via the one-step surfactant-assisted solvothermal method. The impact of surfactant and reducing agent stoichiometry is investigated in this manuscript. Different morphologies and structures of Ni particles, including flower-like nanoflakes, hydrangea-like structures, chain structures, sphere-like structures, and hollow structures were prepared through different processing conditions with two parameters such as temperature and time. Based on scanning electron microscopy (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA) and vibrating sample magnetometry (VSM), the submicron nickel particles show good saturation magnetization and excellent thermal stabilities with a possible growth mechanism for the variety of the structure-tuned formation. Importantly, the microwave absorption properties of the submicron nickel particles were studied. The lowest reflection loss of Ni-P9/T200/H15 with a thin layer thickness of 1.7 mm can reach −42.6 dB at 17.3 GHz.
Specialization of Hepatitis C Virus Envelope Glycoproteins for B Lymphocytes in Chronically Infected Patients

ABSTRACT Hepatitis C virus (HCV) productively infects hepatocytes. Virion surface glycoproteins E1 and E2 play a major role in this restricted cell tropism by mediating virus entry into particular cell types. However, several pieces of evidence have suggested the ability of patient-derived HCV particles to infect peripheral blood mononuclear cells. The viral determinants and mechanisms mediating such events remain poorly understood. Here, we aimed at isolating viral determinants of HCV entry into B lymphocytes. For this purpose, we constructed a library of full E1E2 sequences isolated from serum and B lymphocytes of four chronically infected patients. We observed a strong phylogenetic compartmentalization of E1E2 sequences isolated from B lymphocytes in one patient, indicating that E1E2 glycoproteins can represent important mediators of the strong segregation of two specialized populations in some patients. Most of the E1E2 envelope glycoproteins were functional and allowed transduction of hepatocyte cell lines using HCV-derived pseudoparticles. Strikingly, introduction of envelope glycoproteins isolated from B lymphocytes into the HCV JFH-1 replicating virus switched the entry tropism of this nonlymphotropic virus from hepatotropism to lymphotropism. Significant detection of viral RNA and viral proteins within B cells was restricted to infections with JFH-1 harboring E1E2 from lymphocytes and depended on an endocytic, pH-dependent entry pathway. Here, we achieved for the first time the isolation of HCV viral proteins carrying entry-related lymphotropism determinants. The identification of genetic determinants within E1E2 represents a first step for a better understanding of the complex relationship between HCV infection, viral persistence, and extrahepatic disorders. IMPORTANCE Hepatitis C virus (HCV) mainly replicates within the liver. However, it has been shown that patient-derived HCV particles can slightly infect lymphocytes in vitro and in vivo, highlighting the existence of lymphotropism determinants within HCV viral proteins. We isolated HCV envelope glycoproteins from patient B lymphocytes that conferred to a nonlymphotropic HCV the ability to enter B cells, thus providing a platform for characterization of HCV entry into lymphocytes. This unusual tropism was accompanied by a loss of entry function into hepatocytes, suggesting that HCV lymphotropic variants likely constitute a distinct but parallel source for viral persistence and immune escape within chronically infected patients. Moreover, the level of genetic divergence of B-cell-derived envelopes correlated with their degree of lymphotropism, underlining a long-term specialization of some viral populations for B-lymphocytes. Consequently, the clearance of both hepatotropic and nonhepatotropic HCV populations may be important for effective treatment of chronically infected patients.
The Response of Thalassiosira pseudonana to Long-Term Exposure to Increased CO2 and Decreased pH

The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO2 (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO2, pH 8.1). Batch culture pH changed rapidly because of CO2 (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (<2×105 cells mL−1). After 3 months continuous culture, the C:N ratio was slightly decreased under high CO2 conditions and red fluorescence per cell was slightly increased. However, no change was detected in photosynthetic efficiency (Fv/Fm) or functional cross section of PS II (σPSII). Elevated pCO2 has been predicted to be beneficial to diatoms due to reduced cost of carbon concentration mechanisms. There was reduced transcription of one putative δ-carbonic anhydrase (CA-4) after 3 months growth at increased CO2 but 3 other δ-CAs and the small subunit of RUBISCO showed no change. There was no evidence of adaptation or clade selection of T. pseudonana after ∼100 generations at elevated CO2. On the basis of this long-term culture, pH change of this magnitude in the future ocean may have little effect on T. pseudonana in the absence of genetic adaption.
Braiding operators are universal quantum gates

This paper explores the role of unitary braiding operators in quantum computing. We show that a single specific solution R (the Bell basis change matrix) of the Yang–Baxter equation is a universal gate for quantum computing, in the presence of local unitary transformations. We show that this same R generates a new non-trivial invariant of braids, knots and links. Other solutions of the Yang–Baxter equation are also shown to be universal for quantum computation. The paper discusses these results in the context of comparing quantum and topological points of view. In particular, we discuss quantum computation of link invariants, the relationship between quantum entanglement and topological entanglement, and the structure of braiding in a topological quantum field theory.
Early Sympathy and Social Acceptance Predict the Development of Sharing in Children

Sharing is a fascinating activity of the human species and an important basis for the development of fairness, care, and cooperation in human social interaction. Economic research has proposed that sharing, or the willingness to sacrifice own resources for others, has its roots in social emotions such as sympathy. However, only few cross-sectional experiments have investigated children’s other-regarding preferences, and the question how social-emotional skills influence the willingness to share valuable resources has not been tested. In the present longitudinal-experimental study, a sample of 175 6-year-old children, their primary caregivers, and their teachers is examined over a 3-year period of time. Data are analyzed by means of growth curve modeling. The findings show that sharing valuable resources strongly increases in children from 6 to 9 years of age. Increases in sharing behavior are associated with the early-developing ability to sympathize with anonymous others. Sharing at 7 years of age is predicted by feelings of social acceptance at 6 years of age. These findings hold after controlling for children’s IQ and SES. Girls share more equally than boys at 6 and 7 years of age, however, this gender difference disappears at the age of 9 years. These results indicate that human sharing strongly increases in middle childhood and, that this increase is associated with sympathy towards anonymous others and with feelings of social acceptance. Additionally, sharing develops earlier in girls than in boys. This developmental perspective contributes to new evidence on change in sharing and its social-emotional roots. A better understanding of the factors underlying differences in the development of sharing and pro-social orientations should also provide insights into the development of atypical, anti-social orientations which exhibit social-emotional differences such as aggression and bullying behavior.