Miracle-dz/UniFAR-FTUs huggingface dataset:
IL-4Rα-Independent Expression of Mannose Receptor and Ym1 by Macrophages Depends on their IL-10 Responsiveness

IL-4Rα-dependent responses are essential for granuloma formation and host survival during acute schistosomiasis. Previously, we demonstrated that mice deficient for macrophage-specific IL-4Rα (LysMcreIl4ra−/lox) developed increased hepatotoxicity and gut inflammation; whereas inflammation was restricted to the liver of mice lacking T cell-specific IL-4Rα expression (iLckcreIl4ra−/lox). In the study presented here we further investigated their role in liver granulomatous inflammation. Frequencies and numbers of macrophage, lymphocyte or granulocyte populations, as well as Th1/Th2 cytokine responses were similar in Schistosoma mansoni-infected LysMcreIl4ra−/lox liver granulomas, when compared to Il4ra−/lox control mice. In contrast, a shift to Th1 responses with high IFN-γ and low IL-4, IL-10 and IL-13 was observed in the severely disrupted granulomas of iLckcreIl4ra−/lox and Il4ra−/− mice. As expected, alternative macrophage activation was reduced in both LysMcreIl4ra−/lox and iLckcreIl4ra−/lox granulomas with low arginase 1 and heightened nitric oxide synthase RNA expression in granuloma macrophages of both mouse strains. Interestingly, a discrete subpopulation of SSChighCD11b+I-A/I-EhighCD204+ macrophages retained expression of mannose receptor (MMR) and Ym1 in LysMcreIl4ra−/lox but not in iLckcreIl4ra−/lox granulomas. While aaMφ were in close proximity to the parasite eggs in Il4ra−/lox control mice, MMR+Ym1+ macrophages in LysMcreIl4ra−/lox mice were restricted to the periphery of the granuloma, indicating that they might have different functions. In vivo IL-10 neutralisation resulted in the disappearance of MMR+Ym1+ macrophages in LysMcreIl4ra−/lox mice. Together, these results show that IL-4Rα-responsive T cells are essential to drive alternative macrophage activation and to control granulomatous inflammation in the liver. The data further suggest that in the absence of macrophage-specific IL-4Rα signalling, IL-10 is able to drive mannose receptor- and Ym1-positive macrophages, associated with control of hepatic granulomatous inflammation.
Mild Traumatic Brain Injury (mTBI) and chronic cognitive impairment: A scoping review

Mild traumatic brain injury (mTBI), or concussion, is the most common type of traumatic brain injury. With mTBI comes symptoms that include headaches, fatigue, depression, anxiety and irritability, as well as impaired cognitive function. Symptom resolution is thought to occur within 3 months post-injury, with the exception of a small percentage of individuals who are said to experience persistent post-concussion syndrome. The number of individuals who experience persistent symptoms appears to be low despite clear evidence of longer-term pathophysiological changes resulting from mTBI. In light of the incongruency between these longer-term changes in brain pathology and the number of individuals with longer-term mTBI-related symptoms, particularly impaired cognitive function, we performed a scoping review of the literature that behaviourally assessed short- and long-term cognitive function in individuals with a single mTBI, with the goal of identifying the impact of a single concussion on cognitive function in the chronic stage post-injury. CINAHL, Embase, and Medline/Ovid were searched July 2015 for studies related to concussion and cognitive impairment. Data relating to the presence/absence of cognitive impairment were extracted from 45 studies meeting our inclusion criteria. Results indicate that, in contrast to the prevailing view that most symptoms of concussion are resolved within 3 months post-injury, approximately half of individuals with a single mTBI demonstrate long-term cognitive impairment. Study limitations notwithstanding, these findings highlight the need to carefully examine the long-term implications of a single mTBI.
An examination of critical care nurses' knowledge and attitudes regarding pain management in hospitalized patients.

The purpose of this study was to determine the effectiveness of an educational intervention focused on pain management. The sample consisted of 30 medical/surgical intensive care nurses (age range, 23 to 62 years) employed in a large metropolitan hospital in the southeastern United States. McCaffery and Ferrell's Nurses' Knowledge and Attitude Survey Regarding Pain was administered at 2 points in time: before and after the educational program. The survey simultaneously measures knowledge and attitude levels regarding pain control. The paired t test was used to test for differences between pre- and posttest scores. The Point-biserial and Spearman's correlation coefficients were computed to examine relationships between selected sociodemographic variables and scores (baseline and change) on the survey. Results revealed a significant increase in scores after the educational intervention (t = 9.60. p = .0005). Additionally, a statistically significant correlation was found between change scores (posttest minus pretest scores) and years of nursing experience (rs = .37, p = .047). However, no significant relationships were found between type of nursing degree and baseline or change scores (rpb = .11, p = .575; rpb = .01, p = .955). These results support previous research findings related to the problem of inadequate pain management in the hospital setting. Further, the findings indicate that education regarding pain control is crucial for current nursing students as well as practicing nurses. The challenge for nurses is to be responsive to and integrate current pain management techniques in an effort to decrease the discomfort of hospitalized patients.
Subacute calorie restriction and rapamycin discordantly alter mouse liver proteome homeostasis and reverse aging effects

Calorie restriction (CR) and rapamycin (RP) extend lifespan and improve health across model organisms. Both treatments inhibit mammalian target of rapamycin (mTOR) signaling, a conserved longevity pathway and a key regulator of protein homeostasis, yet their effects on proteome homeostasis are relatively unknown. To comprehensively study the effects of aging, CR, and RP on protein homeostasis, we performed the first simultaneous measurement of mRNA translation, protein turnover, and abundance in livers of young (3 month) and old (25 month) mice subjected to 10‐week RP or 40% CR. Protein abundance and turnover were measured in vivo using 2H3–leucine heavy isotope labeling followed by LC‐MS/MS, and translation was assessed by polysome profiling. We observed 35–60% increased protein half‐lives after CR and 15% increased half‐lives after RP compared to age‐matched controls. Surprisingly, the effects of RP and CR on protein turnover and abundance differed greatly between canonical pathways, with opposite effects in mitochondrial (mt) dysfunction and eIF2 signaling pathways. CR most closely recapitulated the young phenotype in the top pathways. Polysome profiles indicated that CR reduced polysome loading while RP increased polysome loading in young and old mice, suggesting distinct mechanisms of reduced protein synthesis. CR and RP both attenuated protein oxidative damage. Our findings collectively suggest that CR and RP extend lifespan in part through the reduction of protein synthetic burden and damage and a concomitant increase in protein quality. However, these results challenge the notion that RP is a faithful CR mimetic and highlight mechanistic differences between the two interventions.
The spatial resolution of the pinhole eyes of giant clams (Tridacna maxima

Giant clams (Tridacna spp.) have several hundred small pinhole–type eyes on the exposed mantle. They respond by withdrawing the mantle to movements of dark objects, even if these cast no shadow on the animal as a whole. I investigated this ‘sight reaction’ using black and white square–wave gratings whose phase abruptly changed so that the white areas became dark and vice versa. Gratings with periods of 13.5° were ineffective, but gratings of 20.7° caused partial retraction of mantles or siphons. This implies an acceptance angle for the best–resolving eyes of between 8.7° and 21.8°. A single black spot was effective if its angular diameter was 13.5°but not 11.7°. The mean threshold for the pure dimming of a large field was a decrease of 12.3percnt;, but responses increased in strength up to a dimming of 35%. Anatomically the eyes are ca. 400 μm deep from aperture to receptors, the aperture has a mean diameter of 90 μm and the receptors are 25 μm across. This gives an angular acceptance angle for single receptors of 16.5°, which is completely consistent with the behavioural measurements.
Genetics of microtia and associated syndromes

Microtia is a congenital anomaly, characterised by a small, abnormally shaped auricle (pinna). It is usually accompanied by a narrow, blocked or absent ear canal. Microtia can occur as the only clinical abnormality or as part of a syndrome. The estimated prevalence of microtia is 0.8–4.2 per 10 000 births, and it is more common in men. Microtia can have a genetic or environmental predisposition. Mendelian hereditary forms of microtia with an autosomal dominant or recessive mode of inheritance, and some forms due to chromosomal aberrations have been reported. Several responsible genes have been identified, most of them being homeobox genes. Mouse models have been very useful to study these genes, providing valuable information on the development of the auditory system. In this article, we review the epidemiological characteristics of microtia and the environmental causes involved. In addition, we discuss the development of the auditory system, specifically the relevant aspects of external and middle ear development. The focus of this review is to discuss the genetic aspects of microtia and associated syndromes. The clinical aspects of various disorders involving microtia are also discussed in relation to the genes that are causing them.
ASSESSING ADAPTIVE PHENOTYPIC PLASTICITY BY MEANS OF CONDITIONAL STRATEGIES FROM EMPIRICAL DATA: THE LATENT ENVIRONMENTAL THRESHOLD MODEL

Conditional strategies are the most common form of discrete phenotypic plasticity. In a conditional strategy, the phenotype expressed by an organism is determined by the difference between an environmental cue and a threshold, both of which may vary among individuals. The environmental threshold model (ETM) has been proposed as a mean to understand the evolution of conditional strategies, but has been surprisingly seldom applied to empirical studies. A hindrance for the application of the ETM is that often, the proximate cue triggering the phenotypic expression and the individual threshold are not measurable, and can only be assessed using a related observable cue. We describe a new statistical model that can be applied in this common situation. The Latent ETM (LETM) allows for a measurement error in the phenotypic expression of the individual environmental cue and a purely genetically determined threshold. We show that coupling our model with quantitative genetic methods allows an evolutionary approach including an estimation of the heritability of conditional strategies. We evaluate the performance of the LETM with a simulation study and illustrate its utility by applying it to empirical data on the size‐dependent smolting process for stream‐dwelling Atlantic salmon juveniles.
Constitutively Active Cytoplasmic c-Jun N-Terminal Kinase 1 Is a Dominant Regulator of Dendritic Architecture: Role of Microtubule-Associated Protein 2 as an Effector

Normal functioning of the nervous system requires precise regulation of dendritic shape and synaptic connectivity. Here, we report a severe impairment of dendritic structures in the cerebellum and motor cortex of c-Jun N-terminal kinase 1 (JNK1)-deficient mice. Using an unbiased screen for candidate mediators, we identify the dendrite-specific high-molecular-weight microtubule-associated protein 2 (MAP2) as a JNK substrate in the brain. We subsequently show that MAP2 is phosphorylated by JNK in intact cells and that MAP2 proline-rich domain phosphorylation is decreased in JNK1-/- brain. We developed compartment-targeted JNK inhibitors to define whether a functional relationship exists between the physiologically active, cytosolic pool of JNK and dendritic architecture. Using these, we demonstrate that cytosolic, but not nuclear, JNK determines dendritic length and arbor complexity in cultured neurons. Moreover, we confirm that MAP2-dependent process elongation is enhanced after activation of JNK. Using JNK1-/- neurons, we reveal a dominant role for JNK1 over ERK in regulating dendritic arborization, whereas ERK only regulates dendrite shape under conditions in which JNK activity is low (JNK1-/- neurons). These results reveal a novel antagonism between JNK and ERK, potentially providing a mechanism for fine-tuning the dendritic arbor. Together, these data suggest that JNK phosphorylation of MAP2 plays an important role in defining dendritic architecture in the brain.
An O(log n)-Approximation Algorithm for the Edge-Disjoint Paths Problem in Eulerian Planar Graphs

In this article, we study an approximation algorithm for the maximum edge-disjoint paths problem. In this problem, we are given a graph and a collection of pairs of vertices, and the objective is to find the maximum number of pairs that can be connected by edge-disjoint paths. We give an O(log n)-approximation algorithm for the maximum edge-disjoint paths problem when an input graph is either 4-edge-connected planar or Eulerian planar. This improves an O(log2 n)-approximation algorithm given by Kleinberg [2005] for Eulerian planar graphs. Our result also generalizes the result by Chekuri et al. [2004, 2005] who gave an O(log n)-approximation algorithm for the maximum edge-disjoint paths problem with congestion two when an input graph is planar.
Microemulsions as drug delivery systems to improve the solubility and the bioavailability of poorly water-soluble drugs

Importance of the field: Microemulsions have been studied extensively as potential drug delivery vehicles for poorly water-soluble drugs. An understanding of the physicochemical and biopharmaceutical characteristics of the microemulsions according to administration routes will provide guidance for designing the formulations of microemulsions. Areas covered in this review: In this paper, the use and the characteristics of microemulsions as drug delivery vehicles are reviewed. As the formulations of the microemulsion always include a great amount of surfactant and co-surfactant, which may cause hemolysis or histopathological alterations of the tissue, the potential toxicity or the irritancy of microemulsions is also discussed in this paper. What the reader will gain: Developments of microemulsions for poorly water-soluble drugs in recent years are included in this review. Several factors limiting the commercial or clinical use of microemulsions are also discussed. Take home message: Considering the potential in enhanced drug uptake/permeation and facing the limitations, their unique properties make microemulsions a promising vehicle for poorly water-soluble drugs.
Doxazosin versus bendrofluazide: a comparison of the metabolic effects in British South Asians with hypertension.

BACKGROUND People from British South Asian communities have an increased risk of mortality from coronary heart disease (CHD). Doxazosin, a selective alpha(1)-adrenergic blocker, in addition to lowering blood pressure, has been shown to have positive effects on glucose metabolism and lipid profiles in patients with hypertension. AIM We studied doxazosin (1-8 mg) and bendrofluazide (2.5 mg) in patients of British South Asian origin with existing mild to moderate hypertension (doxazosin n = 78; bendrofluazide n = 82), to compare their effects on glucose and lipid metabolism in this group. DESIGN OF STUDY A 34-week randomised, double-blind, parallel-group, multicentre study. SETTING Primary care in the UK. METHOD All doxazosin patients started with an initial dose of 1 mg once daily, titrated to a maximum 8 mg once daily if diastolic blood pressure was >90 mmHg or was not <5 mmHg of the baseline value. The primary efficacy variables were mean glucose and total cholesterol concentrations at week 21. RESULT Doxazosin reduced glucose, total cholesterol, low-density lipoprotein-cholesterol and triglycerides and increased high-density lipoprotein-cholesterol. There were significant differences between doxazosin and bendrofluazide for glucose concentrations at week 21 (P = 0.029) and week 34 (P = 0.015), total cholesterol at week 21 (P = 0.048) and triglycerides at week 21 (P = 0.047) and week 34 (P = 0.009). There was no significant difference in blood pressure lowering between the two treatments. CONCLUSION Doxazosin exhibits beneficial effects on glucose concentrations and lipid profile, in particular in lowering triglyceride concentrations in British South Asians. Whether these desirable characteristics translate to improved overall cardiovascular risk requires formal evaluation.
Progressive Lightcuts for GPU

Lightcuts [Walter et al. 2005] is an attractive rendering algorithm that efficiently handles complex lighting by adaptively approximating the illumination using clusters of virtual point light (VPL) sources. Two of its limitations are the infinite memory footprint required for variance convergence and the bias introduced by VPL contribution clamping. We present a progressive Lightcuts algorithm, which is consistent and converges using a fixed amount of intermediate memory. This is essential for high quality rendering, especially considering the tight memory budget on the GPU.
Loss of Magnetic Insulation in a Crossed-Field Diode: Ion and Collisional Effects

The effect of ion production through ionizing collisions in a magnetically insulated crossed-field gap is studied by using one-dimensional particle-in-cell software. These results are compared with the predictions from previous efforts that assumed immobile sheets of positive charge at different positions within the gap. Our results with mobile ions created via collisions indicate that the diode can lose magnetic insulation of the electron flow at ion densities lower than that predicted from the immobile ion case. Furthermore, we observe that electron scattering plays a significant role in this gap closure. This loss of insulation depends on the background pressure and leads to time-dependent migration of charge across the gap. We characterize both the time-scale and the degree of current transport for cases relevant to the high-power microwave community.
Classifying Learner Engagement through Integration of Multiple Data Sources

Intelligent tutoring systems (ITS) can provide effective instruction, but learners do not always use such systems effectively. In the present study, high school students' action sequences with a mathematics ITS were machine-classified into five finite-state machines indicating guessing strategies, appropriate help use, and independent problem solving; over 90% of problem events were categorized. Students were grouped via cluster analyses based on self reports of motivation. Motivation grouping predicted ITS strategic approach better than prior math achievement (as rated by classroom teachers). Learners who reported being disengaged in math were most likely to exhibit appropriate help use while working with the ITS, relative to average and high motivation learners. The results indicate that learners can readily report their motivation state and that these data predict how learners interact with the ITS.
Location graphs for visual place recognition

With the growing demand for deployment of robots in real scenarios, robustness in the perception capabilities for navigation lies at the forefront of research interest, as this forms the backbone of robotic autonomy. Existing place recognition approaches traditionally follow the feature-based bag-of-words paradigm in order to cut down on the richness of information in images. As structural information is typically ignored, such methods suffer from perceptual aliasing and reduced recall, due to the ambiguity of observations. In a bid to boost the robustness of appearance-based place recognition, we consider the world as a continuous constellation of visual words, while keeping track of their covisibility in a graph structure. Locations are queried based on their appearance, and modelled by their corresponding cluster of landmarks from the global covisibility graph, which retains important relational information about landmarks. Complexity is reduced by comparing locations by their graphs of visual words in a simplified manner. Test results show increased recall performance and robustness to noisy observations, compared to state-of-the-art methods.
Characterization of trabecular bone using the backscattered spectral centroid shift

Ultrasonic attenuation in bone in vivo is generally measured using a through-transmission method at the calcaneus. Although attenuation in calcaneus has been demonstrated to be a useful predictor for osteoporotic fracture risk, measurements at other clinically important sites, such as hip and spine, could potentially contain additional useful diagnostic information. Through-transmission measurements may not be feasible at these sites due to complex bone shapes and the increased amount of intervening soft tissue. Centroid shift from the backscattered signal is an index of attenuation slope and has been used previously to characterize soft tissues. In this paper, centroid shift from signals backscattered from 30 trabecular bone samples in vitro were measured. Attenuation slope also was measured using a through-transmission method. The correlation coefficient between centroid shift and attenuation slope was -0.71. The 95% confidence interval was (-0.86, -0.47). These results suggest that the backscattered spectral centroid shift may contain useful diagnostic information potentially applicable to hip and spine.
Carvedilol dihydrogen phosphate propan-2-ol solvate from powder diffraction data

In the cation of the title compound, C24H27N2O4 +·H2PO4 −·C3H8O [systematic name: 3-(9H-carbazol-4-yloxy)-2-hydroxy-N-[2-(2-methoxyphenoxy)ethyl]propan-1-aminium dihydrogen phosphate propan-2-ol solvate], the mean planes of the tricyclic carbazole system and the benzene ring form a dihedral angle of 42.00 (16)°. In the crystal structure, classical intermolecular O—H⋯O and N—H⋯O hydrogen bonds link the cations, anions and solvent molecules into layers parallel to the ac plane.
The Effect of Link Churn on Wireless Routing

In this paper, we examine the spatiotemporal dynamics of wireless links and evaluate their effect on routing. We ran several experiments using a testbed consisting of 57 MicaZ motes, and collected data on link behavior over one entire day. We use this data to observe the overall network connectivity over time and space. We are able to examine in detail the choice of neighbors and routes using several linkselection mechanisms, both statically and over time. We are able to verify the hairy-edge hypothesis, which states that the most important links for routing are the most difficult to predict. In order to do so, we develop precise definitions of important and unpredictable links. We also find it possible to remove these intermediate links from consideration and still have a very rich set of links to route over, while suffering from fewer difficult-to-predict links. Also, we explore the tradeoff between statically defining routes as opposed to a dynamic protocol. We find that while it is not possible to remove all temporal variations from the network, their impact can be significantly reduced through the use of local redundancy. Finally, we present a survey of how several existing routing protocols fit into the framework developed in the body of the paper.