Miracle-dz/UniFAR-FTUs huggingface dataset:
Apolipoprotein E polymorphism and cardiovascular disease: a HuGE review.

This review examines the association between the apolipoprotein (apo) var epsilon gene polymorphism (or its protein product (apo E)), metabolic regulation of cholesterol, and cardiovascular disease. The apo var epsilon gene is located at chromosome 19q13.2. Among the variants of this gene, alleles (*) epsilon2, (*) epsilon3, and (*) epsilon4 constitute the common polymorphism found in most populations. Of these variants, apo (*) epsilon3 is the most frequent (>60%) in all populations studied. The polymorphism has functional effects on lipoprotein metabolism mediated through the hepatic binding, uptake, and catabolism of chylomicrons, chylomicron remnants, very low density lipoprotein (VLDL), and high density lipoprotein subspecies. Apo E is the primary ligand for two receptors, the low density lipoprotein (LDL) receptor (also known as the B/E receptor) found on the liver and other tissues and an apo E-specific receptor found on the liver. The coordinate interaction of these lipoprotein complexes with their receptors forms the basis for the metabolic regulation of cholesterol. Allelic variation in apo var epsilon is consistently associated with plasma concentrations of total cholesterol, LDL cholesterol, and apo B (the major protein of LDL, VLDL, and chylomicrons). Apo var epsilon has been studied in disorders associated with elevated cholesterol levels or lipid derangements (i.e., hyperlipoproteinemia type III, coronary heart disease, strokes, peripheral artery disease, and diabetes mellitus). The apo var epsilon genotype yields poor predictive values when screening for clinically defined atherosclerosis despite positive, but modest associations with plaque and coronary heart disease outcomes. In addition to genotype-phenotype associations with vascular disease, the alleles and isoforms of apo var epsilon have been related to dementias, most commonly Alzheimer's disease.
Cognitive Flexibility and its Alterations in Neurodegenerative Diseases

Objective: The Wisconsin Card Sorting Test (WCST) is often regarded as a prototypical neuropsychological test of set-shifting ability. However, it has been proposed that WCST performance involves cognitive processes other than set shifting, such as set maintenance and rule inference. Distinguishing between these processes is necessary for the meaningful interpretation of WCST performance deficits in neuropsychological populations. In the present studies, we aimed to concurrently measure processes of set shifting, set maintenance and rule inference in a computerized version of the WCST, and to dissociate these processes based on their dependence on working memory capacity. Method and Results: In Study II-1, we manipulated the number of card-sorting rules to vary the demands placed on working memory-dependent processes of rule inference. As predicted, integration errors as a novel measure of rule-inference efficiency were selectively affected by increasing the number of rules from three to four. In Study II-2, we examined age-related changes in set shifting, set maintenance, and rule inference. We found a specific association between age and integration errors, indicating that rule inference, but not set shifting or set maintenance, is affected in older individuals. Conclusions: Rule inference on WCST-like card-sorting tasks appeared to be selectively impaired when the amount of information to be integrated in working memory increases or when working memory capacity is reduced (as in older individuals). Our findings indicate that measuring integration errors as an index of a distinct rule-inference process can improve the understanding and interpretability of WCST performance.
The impact of homophobia, poverty, and racism on the mental health of gay and bisexual Latino men: findings from 3 US cities.

OBJECTIVES This study assessed the relation between experiences of social discrimination (homophobia, racism, and financial hardship) and symptoms of psychologic distress (anxiety, depression, and suicidal ideation) among self-identified gay and bisexual Latino men in the United States. METHODS Data were collected from a probability sample of 912 men (self-identified as both Latino and nonheterosexual) recruited from the venues and public social spaces identified as both Latino and gay in the cities of Miami, Los Angeles, and New York. RESULTS The study showed high prevalence rates of psychologic symptoms of distress in the population of gay Latino men during the 6 months before the interview, including suicidal ideation (17% prevalence), anxiety (44%), and depressed mood (80%). In both univariate and multivariate analyses, experiences of social discrimination were strong predictors of psychologic symptoms. CONCLUSIONS The mental health difficulties experienced by many gay and bisexual Latino men in the United States are directly related to a social context of oppression that leads to social alienation, low self-esteem, and symptoms of psychologic distress.
The Effects of Reading Serial Books on Children's Appreciation for Quality Literature.

In this study it was hypothesized that if a young reader repeatedly chooses a serial book for pleasure reading, then appreciation and ability to choose and recognize quality works will diminish. Four sixth-grade English classes recorded their independent reading choices for five months. Those who habitually read series books were chosen for the study. These students were then interviewed to determine their reasons for choosing both their series and non-series independent reading and their perceptions on the literacy quality of these books. The findings in this study did not support the hypothesis. (Contains 37 references and 5 tables of data.) (Author/RS) Reproductions supplied by EDRS are the best that can be made from the original document. The Effects of Reading Serial Books on Children's Appreciation for Quality Literature
A RECURSIVE APPROACH TO ROADMAP-BASED PATH PLANNING

Many techniques have been developed to plan paths for mobile robots. The need to provide reasonable paths for mobile robots is critical in the development of useful robots. Many researchers have dismissed roadmap approaches to path planning because of computational inefficiencies and scalability challenges; it is assumed that crude paths result if the problem must be solved in reasonable time. This paper proposes a new approach to path planning that uses recursion to overcome the limitations of other roadmap methods. The JARB algorithm is highly optimized for speed in sparse environments while also providing smooth paths in densely populated environments. By joining recursive branching with the ability to ignore irre levant obstacles, high quality paths can be produced with reduced computation. We describe the algorithm in detail and present experimental results.
MicroRNA expression profiles in non-epithelial ovarian tumors

Ovarian germ cell tumors (OGCTs) and sex cord stromal tumors (SCSTs) are rare gynecologic tumors that are derived from germ and stromal cells, respectively. Unlike their epithelial counterparts, molecular pathogenesis of these tumor types is still poorly understood. Here, we characterized microRNA (miRNA) expression profiles of 9 OGCTs (2 malignant and 7 benign) and 3 SCSTs using small RNA sequencing. We observed significant miRNA expression variations among the three tumor groups. To further demonstrate the biological relevance of our findings, we selected 12 miRNAs for validation in an extended cohort of 16 OGCTs (9 benign and 7 malignant) and 7 SCSTs by reverse transcription-quantitative polymerase chain reaction. Higher expression of miR-373-3p, miR-372-3p and miR-302c-3p and lower expression of miR-199a-5p, miR-214-5p and miR-202-3p were reproducibly observed in malignant OGCTs as compared to benign OGCTs or SCSTs. Comparing with benign OGCTs, miR-202c-3p and miR-513c-5p were more abundant in SCSTs. Additionally, we examined Beclin 1 (BECN1), a target of miR-199a-5p, in the clinical samples using western blot analysis. Our results show that BECN1 expression was higher in malignant OGCTs than benign OGCTs, which is concordant with their lower miR-199a-5p expression. This study suggests that these miRNAs may have potential value as tumor markers and implications for further understanding the molecular basis of these tumor types.
Red cell or serum folate: what to do in clinical practice?

Abstract Folate deficiency has been linked to diverse clinical manifestations and despite the importance of accurate assessment of folate status, the best test for routine use is uncertain. Both serum and red cell folate assays are widely available in clinical laboratories; however, red cell folate is the more time-consuming and costly test. This review sought to evaluate whether the red cell assay demonstrated superior performance characteristics to justify these disadvantages. Red cell folate, but not serum folate, measurements demonstrated analytical variation due to sample pre-treatment parameters, oxygen saturation of haemoglobin and haematocrit. Neither marker was clearly superior in characterising deficiency but serum folate more frequently showed the higher correlation with homocysteine, a sensitive marker of deficiency. Similarly, both serum and red cell folate were shown to increase in response to folic acid supplementation. However, serum folate generally gave the greater response and was able to distinguish different supplementation doses. The C677T polymorphism of methylenetetrahydrofolate reductase alters the distribution of folate forms in red cells and may thereby cause further analytical variability in routine red cell folate assays. Overall, serum folate is cheaper and faster to perform than red cell folate, is influenced by fewer analytical variables and provides an assessment of folate status that may be superior to red cell folate.
A Comparative Study Of The Bbl Crystal Enteric/Nonfermenter Identification System And The Biomerieux Api20e And Api20ne Identification Systems After Overnight Incubation

&NA; The BBL Crystal Enteric/Nonfermenter (Crystal, Becton Dickinson Microbiology Systems) is a new multi‐test identification system for Gram negative rods requiring no oil overlay or addition of reagents. One hundred and three selected Gram negative rod isolates from routine clinical specimens were tested in parallel using the appropriate API20E or API20NE (BioMerieux) reading after overnight incubation. The isolates included in the study, and the number tested, were as follows: Acinetobacter sp, 8; Aeromonas hydrophila, 1; Citrobacter diversus, 1; Citrobacter freundii, 1; Escherichia coli, 13; Enterobacter aerogenes, 10; Enterobacter agglomerans, 2; Enterobacter cloacae, 6; Klebsiella oxytoca, 6; Klebsiella pneumoniae, 12; Morganella morganii, 2; Proteus mirabilis, 6; Pseudomonas aeruginosa, 21; Salmonella sp, 2; Salmonella typhi, 1; Serratia marcescens, 1; Shigella sonnei, 2; Shigella sp, 2; Vibrio metschnikovii, 1; Xanthomonas maltophilia, 3; and, Yersinia enterocolitica, 2. The API20E and API20NE systems combined identified 74.8% (77/103) and the Crystal 97.1% (100/103) of isolates. Twenty six isolates required repeat and/or additional tests for correct identification with the API systems compared to 3 with the Crystal. Crystal is more convenient than API for routine clinical use because it requires fewer repeat and/or extra tests, and is easier and less time consuming to use.
A scalable Reed-Solomon decoding processor based on unified finite-field processing element design

In this paper, we present the design of a scalable Reed-Solomon (RS) codec processor design with a reconfigurable architecture. The reconfigurable architecture has good flexibility for tradeoff between the data throughput rate and power consumption. Compared with the DSP type architecture, the proposed reconfigurable architecture can perform at a higher data throughput rate with shorter latency. Besides, with the combination of two RS processor engines, we can easily double the performance with the scalable design. This good scalability is another advantage of our proposed reconfigurable architecture.
Wedjat: A Mobile Phone Based Medicine In-take Reminder and Monitor

Out-patient medication administration has been identified as the most error-prone procedure in modern health¬care. Under or over doses due to erratic in-takes, drug-drug or drug-food interactions caused by un-reconciled prescriptions and the absence of in-take enforcement and monitoring mechanisms have caused medication errors to become the common cases of all medical errors. Most medication administration errors were made when patients bought different prescribed and over-the-counter medicines from several drug stores and use them at home without little or no guidance. Elderly or chronically ill patients are particularly susceptible to these mistakes. In this paper, we introduce Wedjat, a smart phone application designed to help patients avoiding these mistakes. Wedjat can remind its users to take the correct medicines on time and record the in-take schedules for later review by healthcare professionals. Wedjat has two distinguished features: (1) it can alert the patients about potential drug-drug/drug-food interactions and plan a proper in-take schedule to avoid these interactions; (2) it can revise the in-take schedule automatically when a dose was missed. In both cases, the software always tries to produce the simplest schedule with least number of in-takes. Wedjat is equipped with user friendly interfaces to help its users to recognize the proper medicines and obtain the correct instructions of taking these drugs. It can maintain the medicine in-take records on board, synchronize them with a data¬base on a host machine or upload them onto a Personal Heath Record (PHR) system. A proof-of-concept prototype of Wedjat has been implemented on Window Mobile platform and will be migrated onto Android for Google Phones. This paper introduces the system concept and design principles of Wedjat with emphasis on its medication scheduling algorithms and the modular implementation of mobile computing application.
A Silent Synapse-Based Mechanism for Cocaine-Induced Locomotor Sensitization

Locomotor sensitization is a common and robust behavioral alteration in rodents whereby following exposure to abused drugs such as cocaine, the animal becomes significantly more hyperactive in response to an acute drug challenge. Here, we further analyzed the role of cocaine-induced silent synapses in the nucleus accumbens (NAc) shell and their contribution to the development of locomotor sensitization. Using a combination of viral vector-mediated genetic manipulations, biochemistry, and electrophysiology in a locomotor sensitization paradigm with repeated, daily, noncontingent cocaine (15 mg/kg) injections, we show that dominant-negative cAMP-element binding protein (CREB) prevents cocaine-induced generation of silent synapses of young (30 d old) rats, whereas constitutively active CREB is sufficient to increase the number of NR2B-containing NMDA receptors (NMDARs) at synapses and to generate silent synapses. We further show that occupancy of CREB at the NR2B promoter increases and is causally related to the increase in synaptic NR2B levels. Blockade of NR2B-containing NMDARs by administration of the NR2B-selective antagonist Ro256981 directly into the NAc, under conditions that inhibit cocaine-induced silent synapses, prevents the development of cocaine-elicited locomotor sensitization. Our data are consistent with a cellular cascade whereby cocaine-induced activation of CREB promotes CREB-dependent transcription of NR2B and synaptic incorporation of NR2B-containing NMDARs, which generates new silent synapses within the NAc. We propose that cocaine-induced activation of CREB and generation of new silent synapses may serve as key cellular events mediating cocaine-induced locomotor sensitization. These findings provide a novel cellular mechanism that may contribute to cocaine-induced behavioral alterations.
PARTICIPATION AUX SERVICES SYSTÈME DE PARCS ÉOLIENS MIXTES : APPLICATION EN MILIEU INSULAIRE

Le fort developpement qu'a connu l'energie eolienne cette derniere decennie en Europe se retrouve aujourd'hui au niveau mondial. Cette croissance a ete rendue possible grâce a divers facteurs qui ont guides les volontes de politique energetique en faveur de cette energie. Or un tel developpement amene a repenser son integration dans les systemes d'energie electrique. C'est pourquoi les conditions de raccordement qui lui sont imposees evoluent et tendent a ce qu'elle participe aux services systeme. Ces travaux de these s'interessent a la mise en oeuvre de parcs eoliens bases sur une mixite technologique, capables de satisfaire les nouvelles et futures exigences de raccordement. L'aptitude de differentes technologies d'eoliennes a repondre a differents services est analysee afin de definir les solutions envisageables pour la creation de tels parcs eoliens. Ces travaux se conclus sur une etude d'integration de parcs eoliens mixtes en milieu insulaire.
Anesthesia for cardiac catheterization procedures

Anesthesiologist’s involvement for the purpose of diagnostic and interventional procedures in cardiac catheterization laboratory has been evolving particularly since last two decades. Catheterization laboratory environment poses certain challenges for the anesthesiologist including unfamiliar remote location, exposure to radiation, limited help from colleagues and communication with cardiologists. Anesthesiologists working in catheterization laboratory are required to have adequate knowledge of the environment, personnel, fluoroscope, echocardiography and type of radio contrast dye during the procedure. Anyone who is exposed to radiation environment is expected to protect himself from the exposure and must also wear a dosimeter for cumulative exposure tracing. There is no ideal anesthetic technique and the decision about sedation, general anesthesia or regional anesthesia for the procedure has to be made by attending anesthesiologists in consultation with cardiologists. Anesthesiologists should always try to minimize the effects of anesthesia on cardiovascular system. In addition, oxygenation and ventilatory management should be done according to the diagnostic procedure as it can also influence the diagnosis particularly in pediatric cath procedures. Since more complex procedures are being done in cardiac catheterization laboratory, it is the responsibility of anesthesia department to train and assign dedicated anesthesiologists for new challenges. Role of anesthetist should be well defined so that there is no confrontation during patient management. Sedation in cardiac catheterization laboratory by non-anesthetists is also an issue, which can be sorted out by making policies and protocol in consultation with cardiologists.
Sphingosine-1-phosphate, a novel lipid, involved in cellular proliferation

Sphingosine, a metabolite of membrane sphingolipids, regulates proliferation of quiescent Swiss 3T3 fibroblasts (Zhang, H., N. E. Buckley, K. Gibson. and S. Spiegel. 1990. J. Biol. Chem. 265:76-81). The present study provides new insights into the formation and function of a unique phospholipid, a metabolite of sphingosine, which was unequivocally identified as sphingosine-1-phosphate. The rapid increase in 32P-labeled sphingosine-1-phosphate levels induced by sphingosine was concentration dependent and correlated with its effect on DNA synthesis. Similar to the mitogenic effects of sphingosine, low concentrations of sphingosine-1-phosphate stimulated DNA synthesis and induced pronounced morphological alterations. Both sphingosine and sphingosine-1-phosphate stimulated DNA synthesis in cells made protein kinase C deficient by prolonged treatment with phorbol ester and sphingosine still elicited similar increases in sphingosine-1-phosphate levels in these cells. Although both sphingosine and sphingosine-1- phosphate acted synergistically with a wide variety of growth factors, there was no additive or synergistic effect in response to a combination of sphingosine and sphingosine-1-phosphate. Using a digital imaging system for measurement of calcium changes, we observed that both sphingosine and sphingosine-1-phosphate are potent calcium- mobilizing agonists in viable 3T3 fibroblasts. The rapid rise in cytosolic free calcium was independent of the presence of calcium in the external medium, indicating that the response is due to the mobilization of calcium from internal store. Our results suggest that sphingosine-1-phosphate may be a component of the intracellular second messenger system that is involved in calcium release and the regulation of cell growth induced by sphingosine.
Delayed sleep phase disorder in temporal isolation.

STUDY OBJECTIVES This study sought to characterize sleep and the circadian rhythm of body core temperature of an individual with delayed sleep phase disorder (DSPD) in the absence of temporal cues and social entrainment and to compare those measures to age-matched normal control subjects studied under identical conditions. DESIGN Polysomnography and body temperature were recorded continuously for 4 days in entrained conditions, followed immediately by 17 days in a "free-running" environment. SETTING Temporal isolation facility in the Laboratory of Human Chronobiology, Weill Cornell Medical College. PARTICIPANTS One individual who met criteria for delayed sleep phase disorder according to the International Classification of Sleep Disorders Diagnostic and Coding Manual (ICSD-2) and 3 age-matched control subjects. INTERVENTIONS None. MEASUREMENTS AND RESULTS The DSPD subject had a spontaneous period length (tau) of 25.38 hours compared to an average tau of 24.44 hours for the healthy controls. The DSPD subject also showed an altered phase relationship between sleep/wake and body temperature rhythms, as well as longer sleep latency, poorer sleep efficiency, and altered distribution of slow wave sleep (SWS) within sleep episodes, compared to control subjects. CONCLUSIONS Delayed sleep phase disorder may be the reflection of an abnormal circadian timing system characterized not only by a long tau, but also by an altered internal phase relationship between the sleep/wake system and the circadian rhythm of body temperature. The latter results in significantly disturbed sleep, even when DSPD patients are permitted to sleep and wake at their preferred times.
The Immediate and Long-Term Effects of Singing on the Mood States of People with Traumatic Brain Injury

Mood changes in four male participants with traumatic brain injury (TBI) were observed following their participation in a 15-session song-singing programme. An analysis of the song material was undertaken to categorise the songs according to the predominant mood they portrayed. Results showed significant differences between participants for all moods (p<0.001). Immediate effects were reversed where participants experienced increases in sadness, anger, fear and fatigue. Long-term effects were significant for some participants who reported increased feelings of happiness and decreased feelings of sadness, fear, confusion, tension and fatigue in the long-term. Characteristics of the songs chosen for therapy were typically representative of feelings of sadness. Findings suggest that immediate effects of song-singing intensify and provide cathartic experiences for people with TBI who may have no other space for which to express negative emotions. Long-term effects of song singing have a positive effect on mood state.