Miracle-dz/UniFAR-FTUs huggingface dataset:
Anti-human cytomegalovirus activity of cytokines produced by CD4+ T-cell clones specifically activated by IE1 peptides in vitro

The control of latent cytomegalovirus (CMV) infections by the immune system is poorly understood. We have previously shown that CD4+ T cells specific for the human CMV major regulatory protein IE1 are frequent in latently infected healthy blood donors. In order to learn about the possible role of these cells, we have developed IE1-specific CD4+ T-cell clones and, in this study, analyzed their epitope specificity and function in vitro. We measured their cytokine production when stimulated with specific IE1 peptides or whole recombinant IE1 protein. Their cytokine profiles, as deduced from gamma interferon (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), and interleukin-4 (IL-4) and IL-6 production, were of the Th0- and Th1-like phenotypes. Supernatants from IE1-specific clones producing IFN-gamma and TNF-alpha were shown to inhibit CMV replication in U373 MG cells. This effect was due, as found by using cytokine-specific neutralizing antibodies, mostly to IFN-gamma, which was secreted at higher levels than TNF-alpha. To better assess the anti-CMV activity of cytokines, recombinant IFN-gamma and TNF-alpha were used and shown to have a synergistic effect on the inhibition of CMV replication and protein expression. Thus, IE1-specific CD4+ T cells display in vitro anti-CMV activity through cytokine secretion and may play a role in the control of in vivo latent infections.
Sensation Seeking And Risky Behavior

Risky behavior can be an expression of a normal, genetically influenced personality trait, sensation seeking. Its expression in risky behaviors such as extreme and risky sports, vocations, substance abuse, unsafe sex, and crime, among others, is the topic of this fascinating and accessible book. In "Sensation Seeking and Risky Behavior", Marvin Zuckerman offers a comprehensive view of the role of sensation seeking in a wide range of behaviors, from risky driving and sports through substance use, sex, and crime or other antisocial behaviors. How the personality trait sensation seeking relates to these risky behaviors is described and explained in terms of genetics, biology, attitudes, and expectancies. Insights into prevention and treatment of maladaptive forms of sensation seeking, like substance abuse and unsaf sexual activity, based on the published research, are offered.
Student-written simulation scenarios: a novel cognitive assessment method in a trauma curriculum.

INTRODUCTION Global cognitive and psychomotor assessment in simulation based curricula is complex. We describe assessment of novices' cognitive skills in a trauma curriculum using a simulation aligned facilitated discovery method. METHODS Third-year medical students in a surgery clerkship completed two student-written simulation scenarios (SWSS) as an assessment method in a trauma curriculum employing high fidelity human patient simulators (manikins). SWSS consisted of written physiologic parameters, intervention responses, a performance evaluation form, and a critical interventions checklist. RESULTS Seventy-one students participated. SWSS scores were compared to multiple choice test (MCQ), checklist-graded solo performance in a trauma scenario (STS), and clerkship summative evaluation grades. The SWSS appeared to be slightly better than STS in discriminating between Honors and non-Honors students, although the mean scores of Honors and non-Honors students on SWSS, STS, or MCQ were not significantly different. SWSS exhibited good equivalent form reliability (r=0.88), and higher interrater reliability versus STS (r=0.93 vs r=0.79). CONCLUSION SWSS is a promising assessment method for simulation based curricula.
Optimally profiling and tracing programs

This paper describes algorithms for inserting monitoring code to profile and trace programs. These algorithms greatly reduce the cost of measuring programs with respect to the commonly used technique of placing code in each basic block. Program profiling counts the number of times each basic block in a program executes. Instruction tracing records the sequence of basic blocks traversed in a program execution. The algorithms optimize the placement of counting/tracing code with respect to the expected or measured frequency of each block or edge in a program's control-flow graph. We have implemented the algorithms in a profiling/tracing tool, and they substantially reduce the overhead of profiling and tracing. We also define and study the hierarchy of profiling problems. These problems have two dimensions: what is profiled (i.e., vertices (basic blocks) or edges in a control-flow graph) and where the instrumentation code is placed (in blocks or along edges). We compare the optimal solutions to the profiling problems and describe a new profiling problem: basic-block profiling with edge counters. This problem is important because an optimal solution to any other profiling problem (for a given control-flow graph) is never better than an optimal solution to this problem. Unfortunately, finding an optimal placement of edge counters for vertex profiling appears to be a hard problem in general. However, our work shows that edge profiling with edge counters works well in practice because it is simple and efficient and finds optimal counter placements in most cases. Furthermore, it yields more information than a vertex profile. Tracing also benefits from placing instrumentation code along edges rather than on vertices.
A multiple window-based co-location pattern mining approach for various types of spatial data

Co-location pattern analysis represents the subsets of spatial events whose instances are found in close geographic proximity. Given a collection of Boolean spatial features, the co-location pattern discovery process finds the subsets of features frequently located together. Key challenges in co-location pattern analysis are modelling of neighbourhood in spatial domain, minimum prevalent threshold to generate collocation patterns and analysing extended spatial objects. We discuss the above key challenges using event centric approach and N-most prevalent co-location patterns approach. We propose a window-based model to find the neighbourhood for point spatial datasets and the multiple window model for extended spatial data objects. We also use N-most prevalent co-location patterns approach to filter the number of co-location pattern generation. We propose a more generic and efficient window-based model algorithm to find colocation patterns. Towards the end, we have done a comparative study of the existing approaches with our proposed approach.
Survey of modeling, analysis, and control of hybrid systems

An overview of various modeling frameworks for hybrid systems is given followed by a comparison of the modeling power and the model complexity, which can serve as a guideline for choosing the right model for a given analysis or control problem with hybrid dynamics. Then, the main analydsis and design tasks for hybrid system.s are surveyed together with the methods for their solution, which will be discussed in more detail in subsequent chapters.
Extremely long-term persistence of the McCollough effect.

The question has been raised whether an associative mechanism is responsible for the persistence of the McCollough effect. Since current estimates of its rate of decay are derived from procedures in which subjects are repeatedly tested, it was hypothesized that the measured effects might be attenuated by the testing process. Accordingly, a comparison was made between repeated testing and time-elapse testing. A conventional group of 16 subjects had repeated testing at 0, 8, 24, 56, and 120 hr. after induction. Five other groups of 16 were run, each at one of the time delays, with no intervening tests; an additional measure was taken where appropriate at 120 hr. A magenta-green nulling procedure was used to assess the aftereffect. The repeated-test group showed a linear decrease of effect against the stated delays, reaching zero at 120 hr. In contrast, the time-elapse groups showed little decline up to 120 hr. Those groups retested at 120 hr. showed declines due to prior testing. When four more groups were subsequently tested at intervals up to 2,040 hr. , the effect remained at better than half strength.
Local community detection via flow propagation

We propose a flow propagation algorithm (FlowPro) that finds the community surrounding a node in a complex network. In each iteration of the main process of FlowPro, the initial node propagates a flow that is shared among its neighbors. Each node is able to store, propagate to its neighbors, and return, part of the flow it receives to the initial node. When the algorithm converges, the flow stored in the nodes that belong to the community of the initial node, is generally higher than the flow stored in the rest of the graph nodes, thus the requested community emerges. The novelty of the proposed approach lies in the fact that FlowPro is local, allows to visualize the community and does not require the knowledge of the entire graph as most of the existing methods found in the literature. This makes possible the application of FlowPro in extremely large graphs or in cases where the entire graph is unknown like in most social networks.
Heterogeneity of fibrosis patterns in non‐alcoholic fatty liver disease supports the presence of multiple fibrogenic pathways

Adult non‐alcoholic fatty liver disease (NAFLD) involves lobular necroinflammatory activity and fibrosis is typically centrilobular, whereas paediatric NAFLD has predominantly portal fibrosis. The reasons for these differences are unclear. We aimed to determine (a) how centrilobular and portal fibrosis in children relate to histological parameters; and (b) whether atypical fibrosis patterns exist in adults that are unexplained by current fibrogenesis models.
Utility Maximisation as a Pathway for Maximisation of Happiness

It is hypothesized that pursuit of human happiness is carried out through adaptive change in utility functions by drawing lessons from, in an ex-post manner, the effect of short run utility maximizing choices on utility possibilities. A reference frame for future research is designed to check the truth of this hypothesis which would draw on the methodology already in use for conducting happiness surveys.
From Information Retrieval (IR) to Argument Retrieval (AR) for Legal Cases: Report on a Baseline Study

Users of commercial legal information retrieval (IR) systems often want argument retrieval (AR): retrieving not merely sentences with highlighted terms, but arguments and argument-related information. Using a corpus of argumentannotated legal cases, we conducted a baseline study of current legal IR systems in responding to standard queries. We identify ways in which they cannot meet the need for AR and illustrate how additional argument-relevant information could address some of those inadequacies. We conclude by indicating our approach to developing an AR system to retrieve arguments from legal decisions.
Visual Reliance and Visual Advantage

Visual input improves speech comprehension when auditory signals are degraded due to background noise (Sumby & Pollack, 1954) or hearing impairments (Walden, Prosek, & Worthington, 1975). Listeners strategically put greater focus on visual information to augment impoverished auditory information. This advantage of visual reliance is routinely utilized at clinical settings for adults with neurogenic communication disorders. Individuals experiencing auditory comprehension deficits are frequently encouraged by clinicians to look at speakers’ faces during daily conversations. Despite the presumed advantage of visual information and the routine recommendation on the use of visual cues, there is a lack of research regarding the way individuals with brain lesions utilize visual information.
Studies on the Coumarin Anticoagulant Drugs : Interaction of Human Plasma Albumin and Warfarin Sodium

In studies by continuous flow electrophoresis the coumarin anticoagulant drug warfarin sodium was found to be bound solely to the albumin fraction of the plasma proteins. The interaction was studied in detail by equilibrium dialysis of solutions of crystalline human plasma albumin and warfarin sodium. Analysis of the data showed that albumin possesses a single strong binding site for warfarin with an association constant of 154,000 at 30 C and secondary classes of several sites with a much lower affinity. The free energy of binding for the first anion determined at 3° and 370 C was -6.54 and -7.01 kcal per mole, respectively. The standard enthalpy change for the interaction was -3.48 kcal per mole, and the entropy change was +11.2 U. The negative enthalpy change was surprisingly large and the positive entropy change small for an anion-albumin interaction, suggesting significant nonionic binding. The inability to saturate the albumin binding sites, even when high concentrations of warfarin were used, is consistent with a reversible configurational alteration of the albumin molecule during the binding process. The thermodynamic data indicate that the albumin binding sites for warfarin sodium are formed during the process of binding, rather than being preformed as in antigen-antibody reactions. The strength of the binding process suggests that many of the pharmacodynamic characteristics of warfarin sodium in man are determined by its strong interaction with plasma albumin. Such correlations of the physicochemical interactions and biologic effects of the coumarin anticoagulant drugs should lead to a better understanding of their mechanisms of action.
Induction of Gene Expression as a Monitor of Exposure to Ionizing Radiation

Abstract Amundson, S. A. , Bittner, M. , Meltzer, P., Trent, J. and Fornace, A. J., Jr. Induction of Gene Expression as a Monitor of Exposure to Ionizing Radiation. Radiat. Res. 156, 657–661 (2001). The complex molecular responses to genotoxic stress are mediated by a variety of regulatory pathways. The transcription factor TP53 plays a central role in the cellular response to DNA-damaging agents such as ionizing radiation, but other pathways also play important roles. In addition, differences in radiation quality, such as the exposure to high-LET radiation that occurs during space travel, may influence the pattern of responses. The premise is developed that stress gene responses can be employed as molecular markers for radiation exposure using a combination of informatics and functional genomics approaches. Published studies from our laboratory have already demonstrated such transcriptional responses with doses of γ rays as low as 2 cGy, and in peripheral blood lymphocytes (PBLs) irradiated ex vivo with doses as low as 20 cGy. We have also found several genes elevated in vivo 24 h after whole-body irradiation of mice with 20 cGy. Such studies should provide insight into the molecular responses to physiologically relevant doses, which cannot necessarily be extrapolated from high-dose studies. In addition, ongoing experiments are identifying large numbers of potential biomarkers using microarray hybridization and various irradiation protocols including expression at different times after exposure to low- and high-LET radiation. Computation-intensive informatics analysis methods are also being developed for management of the complex gene expression profiles resulting from these experiments. With further development of these approaches, it may be feasible to monitor changes in gene expression after low-dose radiation exposure and other physiological stresses that may be encountered during manned space flight, such as the planned mission to Mars.
Essential Role for Humoral Immunity during Ehrlichia Infection in Immunocompetent Mice

ABSTRACT Although cellular immunity is essential for host defense during intracellular bacterial infections, humoral immunity can also play a significant role in host defense during infection by some intracellular bacteria, including the ehrlichiae. Antibodies can protect susceptible SCID mice from fatal Ehrlichia chaffeensis infection, an observation that has been hypothesized to involve the opsonization of bacteria released from host cells. To determine whether humoral immunity plays an essential role during ehrlichia infection in immunocompetent mice, we utilized a murine model of fatal monocytotropic ehrlichiosis caused by Ixodes ovatus ehrlichia. Mice lacking either B cells or FcγRI were unable to resolve a low-dose (sublethal) I. ovatus ehrlichia infection, which suggested that humoral immunity is essential for resistance. Polyclonal sera generated in I. ovatus ehrlichia-infected mice recognized a conserved ehrlichia outer membrane protein and, when administered to infected mice, caused a significant decrease in bacterial infection. Mice experimentally depleted of complement, or deficient for complement receptors 1 and 2, were also susceptible to sublethal I. ovatus ehrlichia infection, as were mice that lacked the phox91 subunit of NADPH oxidase. The data are consistent with a mechanism whereby bacteria released from infected cells are lysed directly by complement or undergo antibody-mediated FcγR-dependent phagocytosis and subsequent exposure to reactive oxygen intermediates. The findings suggest mechanisms whereby antibodies contribute to immunity against intracellular bacteria in immunocompetent mice.
Solubility and dissolution enhancement strategies: current understanding and recent trends*

Abstract Identification of lead compounds with higher molecular weight and lower aqueous solubility has become increasingly prevalent with the advent of high throughput screening. Poor aqueous solubility of these lipophilic compounds can drastically affect the dissolution rate and subsequently the drug absorbed in the systemic circulation, imposing a significant burden of time and money during drug development process. Various pre-formulation and formulation strategies have been applied in the past that can improve the aqueous solubility of lipophilic compounds by manipulating either the crystal lattice properties or the activity coefficient of a solute in solution or both, if possible. However, despite various strategies available in the armor of formulation scientist, solubility issue still remains an overriding problem in the drug development process. It is perhaps due to the insufficient conceptual understanding of solubility and dissolution phenomenon that hinders the judgment in selecting suitable strategy for improving aqueous solubility and/or dissolution rate. This article, therefore, focuses on (i) revisiting the theoretical and mathematical concepts associated with solubility and dissolution, (ii) their application in making rationale decision for selecting suitable pre-formulation and formulation strategies and (iii) the relevant research performed in this field in past decade.
Clinical Use of the Antiseptic Polihexanide for Genital Tract Infections

Background: In clinical practice, treatment of genital tract infections is based on administration of either antibiotics or antiseptics. While antibiotics may be applied systemically or topically, antiseptics may be applied only topically. In case of bacterial vaginosis (BV), antibiotic therapy may often be limited and side effects due to systemic administration may develop. Polihexanide (PHMB) is a promising option for the topical treatment of genital tract infections, in particular BV and vaginitis. Method: A systematic search for publications on the use of PHMB for the treatment of genital infections in two electronic databases was performed. Titles, abstracts and citations were imported into a reference database. Duplicates were removed and two reviewers assessed each identified publication separately. Results: Among a total of 204 references, 3 prospective randomized trials were identified. Two trials treated BV infections with PHMB in comparison to clindamycin as antibiotic standard therapy with no significant differences either in safety or in efficacy. The third controlled trial investigated the clinical efficacy of PHMB compared to placebo in the treatment of human papilloma virus. Patients treated with PHMB daily for up to 16-weeks showed significantly higher (52%) clearance of genital warts as compared to patients treated with placebo (4%). Conclusion: PHMB may be a clinically effective alternative for the treatment of BV and human papilloma virus. Although PHMB-based antiseptics are available since the late 90s, controlled trials to investigate its clinical potential for antiseptic treatment are scant. Clinical use of antiseptics for the treatment of infectious diseases should be explored and supported further.
On Model Predictive Control of Quasi-Resonant converters

In this paper, different model predictive control synthesis frameworks are examined for DC–DC quasi-resonant converters in order to achieve stability and desired performance. The performances of model predictive control strategies which make use of different forms of linearized models are compared. These linear models are ranging from a simple fixed model, linearized about a reference steady state to a weighted sum of different local models called multi model predictive control. A more complicated choice is represented by the extended dynamic matrix control in which the control input is determined based on the local linear model approximation of the system that is updated during each sampling interval, by making use of a nonlinear model. In this paper, by using and comparing these methods, a new control scheme for quasi-resonant converters is described. The proposed control strategy is applied to a typical half-wave zero-current switching QRC. Simulation results show an excellent transient response and a good tracking for a wide operating range and uncertainties in modeling.
Rejection of bone marrow allografts by mice with severe combined immune deficiency (SCID). Evidence that natural killer cells can mediate the specificity of marrow graft rejection

C.B-17 scid (H-2d) mice are homozygous for the gene that causes severe combined immune deficiency (SCID). These mice have no T or B cell function, yet display normal natural killer (NK) activity. Irradiated SCID mice were challenged with marrow grafts to determine if antibodies are necessary for marrow allograft rejection. SCID mice rejected H-2/Hh- 1 allogeneic marrow grafts. Moreover, this rejection capability could be adoptively transferred using SCID marrow as a source of NK progenitors infused into irradiated B6 (H-2b) hosts. We conclude that NK cells can mediate marrow allograft reactivity in the absence of immunoglobulin. It follows that NK cells probably have specific receptors for Hh antigens.
Implications of Interdomain Traffic Characteristics on Traffic Engineering

We study the interdomain traffic as seen by a non-transit ISP, based on a six days trace covering all the interdomain links of this ISP. Our analysis considers the relationships between the interdomain traffic and the interdomain topology. We first discuss the day-to-day stability of the interdomain traffic matrix to evaluate the feasibility of interdomain traffic engineering. Then, we study the variability of the interdomain flows for several aggregation levels (prefix, AS and sink tree) and with respect to the interdomain topology seen by BGP. We show that despite the important variability of interdomain flows, it would be useful for a non-transit ISP to traffic engineer its access traffic by relying on a sink tree aggregation level.