Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression pattern of the AP‐1 family in breast cancer: Association of fosB expression with a well‐differentiated, receptor‐positive tumor phenotype

In the present study, the expression of members of the AP‐1 family of transcription factors in breast tumors (n = 53) was investigated by Western blot with antibodies specific for each of the AP‐1 family members (c‐jun, junB, junD and c‐fos, fosB, fra1 and fra2). The tumors were characterized with regard to grading, staging, histology, steroid‐receptor‐expression status and c‐erbB2/neu expression. For comparison, normal breast‐tissue samples, human breast‐cancer cell lines (T47D and MDA‐MB231) and the transformed human breast epithelial cell line HBL100 were also analyzed. For c‐jun, junB, c‐fos and fra2, a relatively uniform expression pattern without significant differences among tumors was observed. junD‐protein amounts varied strongly in the tumor specimens. fosB‐expression levels also varied strongly in the tumors, weak/absent expression being found in 47%, while 45% exhibited strong/very strong levels of expression. While none of the other AP‐1 family members showed significant correlations with clinico‐pathological tumor parameters or receptor status, expression of fosB was found to correlate significantly with positive steroid‐hormone‐receptor status (in the tumors and the cell lines) and a more differentiated tumor phenotype. Expression of 2 fra‐1‐specific bands of 33 and 36.5 kDa showed significant negative correlation with fosB expression, as well as with estrogen‐receptor status and differentiation. We conclude that strong differences in the expression pattern of AP‐1 family members are present in breast tumors, and that certain members of this family, such as fosB and fra‐1, might be involved in the pathogenesis of these tumors. Int. J. Cancer (Pred. Oncol.) 84:533–538, 1999. © 1999 Wiley‐Liss, Inc.
Postoperative Ischemic Optic Neuropathy

Study Design. Systematic literature review. Objective. To determine if there are predictors or preventative measures for postoperative ischemic optic neuropathy (ION) associated with spine surgery. Summary of Background Data. Postoperative ION is a devastating complication that is most common after cardiac and spinal fusion surgery. Identifying patient or perioperative predictors for postoperative ION could lead to therapeutic modifications designed to minimize its occurrence. Methods. A systematic literature review was conducted in MEDLINE, EMBASE, and the Cochrane Collaboration Library for literature published in English from 1990 through 2008 reporting on ION following spine surgery. References from review articles of ION were used, but articles without original material were excluded. Data on study design, patient demographics, and perioperative characteristics were collected and analyzed. Two independent reviewers assessed the strength of literature using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) criteria assessing quality, quantity, and consistency of results. Disagreements were resolved by consensus. Results. Nineteen of 360 articles on postoperative ION after spine surgery met inclusion/exclusion criteria. The quality of evidence was very low as the majority of articles were case reports. The majority of ION patients were men between 30 and 69 years, undergoing spinal fusion surgery with an operative duration greater than 5 hours and an estimated blood loss greater than 1 L. Confounding factors and lack of a denominator from the case reports and case series precluded identification of risk factors with even a modest level of evidence. Conclusion. Postoperative ION after spinal surgery is a rare event, which may be associated with prone position surgery of more than 5 hours surgical duration and blood loss of more than 1 L. Informing patients of this remote risk should be considered during preoperative counseling. The quality of evidence for preventative measures for postoperative ION after spinal fusion surgery is very low, but it has been proposed that efforts aimed at reducing the duration or severity of venous congestion in the head may be beneficial.
[Childhood insomnia].

AIMS This work reviews the causes of insomnia in children and attempts to show the difficulties involved in performing a diagnosis to differentiate it from other sleep disorders. METHOD Insomnia is a disorder in which the sufferer has difficulty in falling or staying asleep. There are multifactorial causes involved in the aetiology of childhood insomnia. In order to understand and diagnose insomnia in childhood it is necessary to have a good knowledge of the mechanisms involved in the genesis of sleeping and waking. We must also take the possible existence of other types of disorders into consideration. Insomnia appears as an imbalance between the systems that maintain the waking state and those that are in charge of activating sleep generating systems. In children, this imbalance depends on the state of functional immaturity peculiar to each age. Rather than a shortage of sleep, childhood insomnia takes the form of a lack of matching between the child s own rhythm or need to sleep and his or her family or social environment. Insomnia is most frequently caused by environmental, behavioural and psychological factors. A correct diagnosis of childhood insomnia has to be based on determining a procedure to differentiate it from parasomnias or motor disorders affecting waking and sleep. CONCLUSIONS Identifying the causes of childhood insomnia, differentiating it from real insomnia and other sleep disorders, and resolving it are no simple matter because of the influence exerted by the environment and the child s behaviour on how he or she sleeps.
Thermally Stable and Regenerable Platinum–Tin Clusters for Propane Dehydrogenation Prepared by Atom Trapping on Ceria

Abstract Ceria (CeO2) supports are unique in their ability to trap ionic platinum (Pt), providing exceptional stability for isolated single atoms of Pt. The reactivity and stability of single‐atom Pt species was explored for the industrially important light alkane dehydrogenation reaction. The single‐atom Pt/CeO2 catalysts are stable during propane dehydrogenation, but are not selective for propylene. DFT calculations show strong adsorption of the olefin produced, leading to further unwanted reactions. In contrast, when tin (Sn) is added to CeO2, the single‐atom Pt catalyst undergoes an activation phase where it transforms into Pt–Sn clusters under reaction conditions. Formation of small Pt–Sn clusters allows the catalyst to achieve high selectivity towards propylene because of facile desorption of the product. The CeO2‐supported Pt–Sn clusters are very stable, even during extended reaction at 680 °C. Coke formation is almost completely suppressed by adding water vapor to the feed. Furthermore, upon oxidation the Pt–Sn clusters readily revert to the atomically dispersed species on CeO2, making Pt–Sn/CeO2 a fully regenerable catalyst.
Industrial Value Added and Carbondioxide Emissions: A Cross-Country Comparison

This study aims to investigate the relationship between industrial value added and CO2 emissions for developing and developed countries with the aim of making a comparison. Many countries ignore the environmental sensitivity not to drop back due to the competitiveness. However, this recklessness emerges crucial environmental pollution which contributes to the danger of global warming. Not only developing countries cause pollution but also many developed countries do. However, it is more likely that developed countries would like to consider the importance of the environment. Our empirical findings show that there is immediate need to search for alternatives production techniques regardless of country development stage.
Prospective validation of the Rockall risk scoring system for upper GI hemorrhage in subgroups of patients with varices and peptic ulcers

OBJECTIVES:The Rockall risk assessment score was devised to allow prediction of the risk of rebleeding and death in patients with upper GI hemorrhage. The score was derived by multivariate analysis in a cohort of patients with upper GI hemorrhage and subsequently validated in a second cohort. Only 4.4% of patients included in the initial study had esophageal varices, and analysis was not performed according to the etiology of the bleeding. Our aim was to assess the validity of the Rockall risk scoring system in predicting rebleeding and mortality in patients with esophageal varices or peptic ulcers.METHODS:Admissions (n = 358) over 32 months to a single specialist GI bleeding unit were scored prospectively. The distribution of episodes of rebleeding and mortality by Rockall score were statistically analyzed using Fisher's exact test with 99% CIs calculated using a Monte Carlo method. The Child-Pugh score was determined in patients with esophageal varices.RESULTS:The Rockall score was predictive of both rebleeding and mortality in patients with variceal hemorrhage (both ps < 0.0005), as was the Child-Pugh score (p = 0.001 and p < 0.0005, respectively). The initial Rockall score was predictive of mortality in patients with peptic ulcers (p = 0.01), although the complete score was not (p > 0.05). The complete score did, however, predict rebleeding in these patients (p = 0.001). CONCLUSION:This is the first study to validate the Rockall score in specific subgroups of patients with esophageal varices or peptic ulcers and suggests that it is particularly applicable to variceal hemorrhage.
Augmented invariance control for systems with smoothness constraints

In many applications, the controlled dynamical system is subject to constraints, e.g. to enforce a desired performance. There are various methods, which guarantee the enforcement of such constraints. They do, however, not provide satisfactory solutions if the system state has to exhibit certain smoothness properties. This work presents a novel method of designing a control input, which guarantees the adherence to constraints as well as a desired smoothness of the system state. The control scheme is implemented as an add-on to an existing task dependent control law. Augmentation of the dynamical system achieves a desired smoothness of the state whereas methods from invariance control are utilized to guarantee constraint enforcement.
Peanut Clump Virus RNA-1-Encoded P15 Regulates Viral RNA Accumulation but Is Not Abundant at Viral RNA Replication Sites

ABSTRACT RNA-1 of peanut clump pecluvirus (PCV) encodes N-terminally overlapping proteins which contain helicase-like (P131) and polymerase-like (P191) domains and is able to replicate in the absence of RNA-2 in protoplasts of tobacco BY-2 cells. RNA-1 also encodes P15, which is expressed via a subgenomic RNA. To investigate the role of P15, we analyzed RNA accumulation in tobacco BY-2 protoplasts inoculated with RNA-1 containing mutations in P15. For all the mutants, the amount of progeny RNA-1 produced was significantly lower than that obtained for wild-type RNA-1. If RNA-2 was included in the inoculum, the accumulation of both progeny RNAs was diminished, but near-normal yields of both could be recovered if the inoculum was supplemented with a small, chimeric viral replicon expressing P15, demonstrating that P15 has an effect on viral RNA accumulation. To further analyze the role of P15, transcripts were produced expressing P15 fused to enhanced green fluorescent protein (EGFP). Following inoculation to protoplasts, epifluorescence microscopy revealed that P15 accumulated as spots around the nucleus and in the cytoplasm. Intracellular sites of viral RNA synthesis were visualized by laser scanning confocal microscopy of infected protoplasts labeled with 5-bromouridine 5′-triphosphate (BrUTP). BrUTP labeling also occured in spots distributed within the cytoplasm and around the nucleus. However, the BrUTP-labeled RNA and EGFP/P15 very rarely colocalized, suggesting that P15 does not act primarily at sites of viral replication but intervenes indirectly to control viral accumulation levels.
Real-time indoor patient movement pattern telemonitoring with one-meter precision

Monitoring of the activities of daily living of the elderly at home is widely recognized as useful for detection of new or deteriorated health conditions. However, the accuracy of existing indoor location tracking systems remains unsatisfactory. The aim of this study was therefore to develop a localization system that can identify a patient's real-time location in a home environment with maximum estimation error of two meters at a 95% confidence level. A prototype based on a sensor fusion approach was built. This involved the development of both a step detector using the accelerometer and compass of an iPhone 5, and a radio-based localization subsystem using Kalman filter and received signal strength indication (RSSI). The results of our experiments were promising with average estimation error of 0.55 meters. We are confident that with more work our design can be adapted to a home-like environment with a more robust localization solution.
Endothelial/Pericyte Interactions

Interactions between endothelial cells and mural cells (pericytes and vascular smooth muscle cells) in the blood vessel wall have recently come into focus as central processes in the regulation of vascular formation, stabilization, remodeling, and function. Failure of the interactions between the 2 cell types, as seen in numerous genetic mouse models, results in severe and often lethal cardiovascular defects. Abnormal interactions between the 2 cell types are also implicated in a number of human pathological conditions, including tumor angiogenesis, diabetic microangiopathy, ectopic tissue calcification, and stroke and dementia syndrome CADASIL. In the present review, we summarize current knowledge concerning the identity, characteristics, diversity, ontogeny, and plasticity of pericytes. We focus on the advancement in recent years of the understanding of intercellular communication between endothelial and mural cells with a focus on transforming growth factor α, angiopoietins, platelet-derived growth factor, spingosine-1-phosphate, and Notch ligands and their respective receptors. We finally highlight recent important data contributing to the understanding of the role of pericytes in tumor angiogenesis, diabetic retinopathy, and hereditary lymphedema.
Force-dependent hopping rates of RNA hairpins can be estimated from accurate measurement of the folding landscapes

The sequence-dependent folding landscapes of nucleic acid hairpins reflect much of the complexity of biomolecular folding. Folding trajectories, generated by using single-molecule force-clamp experiments by attaching semiflexible polymers to the ends of hairpins, have been used to infer their folding landscapes. Using simulations and theory, we study the effect of the dynamics of the attached handles on the handle-free RNA free-energy profile Foeq(zm), where zm is the molecular extension of the hairpin. Accurate measurements of Foeq(zm) requires stiff polymers with small L/lp, where L is the contour length of the handle, and lp is the persistence length. Paradoxically, reliable estimates of the hopping rates can only be made by using flexible handles. Nevertheless, we show that the equilibrium free-energy profile Foeq(zm) at an external tension fm, the force (f) at which the folded and unfolded states are equally populated, in conjunction with Kramers' theory, can provide accurate estimates of the force-dependent hopping rates in the absence of handles at arbitrary values of f. Our theoretical framework shows that zm is a good reaction coordinate for nucleic acid hairpins under tension.
Host-Adapted Cryptosporidium spp. in Canada Geese (Branta canadensis)

ABSTRACT The prevalence and distribution of Cryptosporidium spp. in the fecal droppings of the free-living waterfowl Canada geese were examined at 13 sites in Ohio and Illinois. On the basis of the analysis of the small-subunit rRNA gene by PCR, followed by restriction fragment length polymorphism analysis and DNA sequencing, 49 (23.4%) of 209 fecal specimens collected from 10 sites (76.9%) were positive for Cryptosporidium spp. The following five Cryptosporidium species and genotypes were identified: Cryptosporidium goose genotype I (in 36 specimens), Cryptosporidium goose genotype II (in 9 specimens), Cryptosporidium duck genotype (in 1 specimen), Cryptosporidium parvum (in 4 specimens), and C. hominis (in 2 specimens). Cryptosporidium goose genotype I was the most prevalent parasite and was found at all five Cryptosporidium-positive sites in Ohio and at four of five positive sites in Illinois, followed by Cryptosporidium goose genotype II, which was found at two of five positive sites in Ohio and at four of five positive sites in Illinois. Cryptosporidium goose genotype II was detected for the first time, and it is phylogenetically related to goose genotype I and the duck genotype. All three genotypes have not so far been reported in humans, and their pathogenicity in geese has not been determined. Only 10.2% of the Cryptosporidium-positive specimens had C. parvum and C. hominis. The results of this study indicate that Canada geese might only serve as accidental carriers of cryptosporidia infectious to humans and probably play a minor role in the animal-to-human transmission cycle of the pathogen.
Cirrosporium novae-zelandiae, an enigmatic coelomycete with meristem arthroconidia, with ancestors in the Eurotiomycetes

A culture of Cirrosporium novae-zelandiae, the type species of a distinctive, monotypic coelomycete genus, was isolated from a specimen collected near the holotype locality in New Zealand. Light microscopic and environmental scanning electron microscopic observations confirm the details of the unusual meristem arthric conidium ontogeny presented in the protolog. For phylogenetic analysis, a dataset of 122 species representing nine classes of euascomycetes was assembled including sequences from nuclear small and large subunits (nc18S, nc28S) and mitochondrial small subunit (mr16S) ribosomal RNA and the largest and second largest subunits of RNA polymerase II (RPB1, RPB2). A five-gene phylogeny suggests that the fungus is phylogenetically related to the Eurotiomycetes. It sits alone on a long branch as a sister to the Mycocaliciales of the Mycocaliciomycetidae. Cirrosporium exhibits several morphological characters similar to those of members of the Mycocaliciales; however, the paucity of known anamorphs in this order does not offer any further clarification on possible relationships. It is clear that the rare and broadly distributed meristem arthric ontogenetic pattern is polyphyletic, occurring in widely separate groups of anamorphs of both the Ascomycota and Basidiomycota.
Retrieving Precipitable Water Vapor From Shipborne Multi‐GNSS Observations

Precipitable water vapor (PWV) is an important parameter for climate research and a crucial factor to achieve high accuracy in satellite geodesy and satellite altimetry. Currently Global Navigation Satellite System (GNSS) PWV retrieval using static Precise Point Positioning is limited to ground stations. We demonstrated the PWV retrieval using kinematic Precise Point Positioning method with shipborne GNSS observations during a 20‐day experiment in 2016 in Fram Strait, the region of the Arctic Ocean between Greenland and Svalbard. The shipborne GNSS PWV shows an agreement of ~1.1 mm with numerical weather model data and radiosonde observations, and a root‐mean‐square of ~1.7 mm compared to Satellite with ARgos and ALtiKa PWV. An improvement of 10% is demonstrated with the multi‐GNSS compared to the Global Positioning System solution. The PWV retrieval was conducted under different sea state from calm water up to gale. Such shipborne GNSS PWV has the promising potential to improve numerical weather forecasts and satellite altimetry.
Well-being, affective commitment and job satisfaction: influences on turnover intentions in casual dining employees

ABSTRACT This case study examines a large franchise of an American style casual dining chain. The purpose of this research was to determine whether new changes to the operational procedures of a restaurant company facilitated positive or negative workplace responses. Specifically, the way the changes influenced well-being, affective commitment, job satisfaction, and turnover intentions was examined. Employees and managers from the 42-unit franchise yielded 270 completed responses. A mixed methods approach, utilizing PROCESS modeling and content analysis, was employed to determine the results of the study. The PROCESS modeling showed that workplace well-being significantly influenced turnover intentions when mediated by positive affective commitment, negative affective commitment, and job satisfaction. The qualitative analysis of the open-ended questions demonstrated themes of environment and responsibilities, with subthemes of management, teamwork, staff, and pay vs. work, Tablet X, and menu changes. Implications for practice are noted indicating that organizations should implement strategies that will bring about more positive emotional connections with their employees, while simultaneously reducing the negative emotions that employees have about their work environment. Further academic and practical implications are discussed.
Spinal Cord Stimulation versus Repeated Lumbosacral Spine Surgery for Chronic Pain: A Randomized, Controlled Trial

OBJECTIVE:Persistent or recurrent radicular pain after lumbosacral spine surgery is often associated with nerve root compression and is treated by repeated operation or, as a last resort, by spinal cord stimulation (SCS). We conducted a prospective, randomized, controlled trial to test our hypothesis that SCS is more likely than reoperation to result in a successful outcome by standard measures of pain relief and treatment outcome, including subsequent use of health care resources. METHODS:For an average of 3 years postoperatively, disinterested third-party interviewers followed 50 patients selected for reoperation by standard criteria and randomized to SCS or reoperation. If the results of the randomized treatment were unsatisfactory, patients could cross over to the alternative. Success was based on self-reported pain relief and patient satisfaction. Crossover to the alternative procedure was an outcome measure. Use of analgesics, activities of daily living, and work status were self-reported. RESULTS:Among 45 patients (90%) available for follow-up, SCS was more successful than reoperation (9 of 19 patients versus 3 of 26 patients, P < 0.01). Patients initially randomized to SCS were significantly less likely to cross over than were those randomized to reoperation (5 of 24 patients versus 14 of 26 patients, P = 0.02). Patients randomized to reoperation required increased opiate analgesics significantly more often than those randomized to SCS (P < 0.025). Other measures of activities of daily living and work status did not differ significantly. CONCLUSION:SCS is more effective than reoperation as a treatment for persistent radicular pain after lumbosacral spine surgery, and in the great majority of patients, it obviates the need for reoperation.