Miracle-dz/UniFAR-FTUs huggingface dataset:
On the controllability of evaluative-priming effects: Some limits that are none

Two experiments examined recent claims of uncontrollability of the evaluative-priming effect. According to these claims, imposing an adaptive 600 ms response deadline prevents successful faking (Degner, 2009). Furthermore, strategic control attempts have been argued not to reduce the priming measure's sensitivity to spontaneous evaluations so that correlations of evaluative-priming effects with external criteria are not affected by attempts to fake (Bar-Anan, 2010). Here, we show that faking is possible even with an adaptive 600 ms response deadline when faking instructions do not conflict with speed pressures imposed thereby (Experiments 1 and 2). In addition, suitable faking instructions substantially affect the predictive validity of priming effects in terms of their correlations with (non-faked) self-report measures and the Implicit Association Test (Experiment 2). The previous claims about the uncontrollability of the evaluative-priming effect may thus have been premature.
Detection of Red Blood Cell in Urine Micrograph

The classical method of red cells examination in urine micrograph is counted by manpower, which has the following deficiencies such as low efficiency, strong subjectivity and poor reliability. This paper focuses on the detection of red blood cells in urine image captured under microscope by image processing. After the urine image is preprocessed by improved Sobel operator, red blood cells are localized using Hough transform and their features, which are extracted and selected by principal component analysis (PCA), can be classified with LDA (linear discriminant analysis). It provides a reliable and convenient detective method for medical research.
Multistate Markov models for disease progression with classification error

Summary. Many chronic diseases have a natural interpretation in terms of staged progression. Multistate models based on Markov processes are a well-established method of estimating rates of transition between stages of disease. However, diagnoses of disease stages are sometimes subject to error. The paper presents a general hidden Markov model for simultaneously estimating transition rates and probabilities of stage misclassification. Covariates can be fitted to both the transition rates and the misclassification probabilities. For example, in the study of abdominal aortic aneurysms by ultrasonography, the disease is staged by severity, according to successive ranges of aortic diameter. The model is illustrated on data from a trial of aortic aneurysm screening, in which the screening measurements are subject to error. General purpose software for model implementation has been developed in the form of an R package and is made freely available.
Adapting haptic guidance authority based on user grip

Haptic guidance systems support the operator in task execution using additional forces on the input device. Scaling of the guidance forces determines the control authority of the support system. As task complexity may vary, one level of the guidance scaling may be insufficient, and adaptation of the control authority may be helpful. The available literature mostly proposed to adapt the authority based on external cues (e.g. actual performance or safety) and the user had no direct way to modulate the desired level of support. In this paper we investigated a variable authority guidance scheme based on the user grip force. During a user study (with 8 subjects) we explored two opposite approaches to trade the control authority (i.e. increasing or decreasing guidance force magnitude with increased user grip). To simulate increased task difficulty and imperfections of the haptic guidance system, at random times either an unpredictable force disturbance was added or subjects were presented with incorrect guidance. While the performance differences between the fixed- and the variable-authority schemes were not significant, the “decreasing guidance with increased user grip” scheme allowed to substantially reduce the user control effort (steering force), especially when the guidance system was incorrect. The presented method essentially provides additional control over the guidance system without reducing the performance.
Suppression of Endotoxin- and Staphylococcal Exotoxin-Induced Cytokines and Chemokines by a Phospholipase C Inhibitor in Human Peripheral Blood Mononuclear Cells

ABSTRACT Excessive release of proinflammatory cytokines from cells stimulated with lipopolysaccharide (LPS) or staphylococcal exotoxin (SE) mediates the pathophysiologic manifestations of septic shock. Tricyclodecan-9-yl (D609), an inhibitor of phosphatidylcholine-specific phospholipase C, suppressed LPS- or SE-induced cytokines and chemokines in human peripheral blood mononuclear cells. These data suggest a potential role for D609 in the treatment of septic shock.
Fendiline Mobilizes Intracellular Ca2+ In Chang Liver Cells

1. The effects of the antianginal drug fendiline (N‐[3,3‐diphenylpropyl]‐α‐methyl‐benzylamine) on intracellular free Ca2+ levels ([Ca2+]i) in Chang liver cells were evaluated using fura‐2 as a fluorescent Ca2+ indicator.
Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication

ABSTRACT Dengue virus is the most prevalent cause of arthropod-borne infection worldwide. Due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. Host RNA-binding proteins mediate various aspects of virus replication through their physical interactions with viral RNA. Here we describe a technique designed to identify such interactions in the context of infected cells using UV cross-linking followed by antisense-mediated affinity purification and mass spectrometry. Using this approach, we identified interactions, several of them novel, between host proteins and dengue viral RNA in infected Huh7 cells. Most of these interactions were subsequently validated using RNA immunoprecipitation. Using small interfering RNA (siRNA)-mediated gene silencing, we showed that more than half of these host proteins are likely involved in regulating virus replication, demonstrating the utility of this method in identifying biologically relevant interactions that may not be identified using traditional in vitro approaches. IMPORTANCE Dengue virus is the most prevalent cause of arthropod-borne infection worldwide. Viral RNA molecules physically interact with cellular RNA-binding proteins (RBPs) throughout the course of infection; the identification of such interactions will lead to the elucidation of the molecular mechanisms of virus replication. Until now, the identification of host proteins bound to dengue viral RNA has been accomplished using in vitro strategies. Here, we used a method for the specific purification of dengue viral ribonucleoprotein (RNP) complexes from infected cells and subsequently identified the associated proteins by mass spectrometry. We then validated a functional role for the majority of these proteins in mediating efficient virus replication. This approach has broad relevance to virology and RNA biology, as it could theoretically be used to purify any viral RNP complex of interest. Dengue virus is the most prevalent cause of arthropod-borne infection worldwide. Viral RNA molecules physically interact with cellular RNA-binding proteins (RBPs) throughout the course of infection; the identification of such interactions will lead to the elucidation of the molecular mechanisms of virus replication. Until now, the identification of host proteins bound to dengue viral RNA has been accomplished using in vitro strategies. Here, we used a method for the specific purification of dengue viral ribonucleoprotein (RNP) complexes from infected cells and subsequently identified the associated proteins by mass spectrometry. We then validated a functional role for the majority of these proteins in mediating efficient virus replication. This approach has broad relevance to virology and RNA biology, as it could theoretically be used to purify any viral RNP complex of interest.
The Drosophila Basic Helix-Loop-Helix Protein DIMMED Directly Activates PHM, a Gene Encoding a Neuropeptide-Amidating Enzyme

ABSTRACT The basic helix-loop-helix (bHLH) protein DIMMED (DIMM) supports the differentiation of secretory properties in numerous peptidergic cells of Drosophila melanogaster. DIMM is coexpressed with diverse amidated neuropeptides and with the amidating enzyme peptidylglycine α-hydroxylating monooxygenase (PHM) in approximately 300 cells of the late embryo. Here we confirm that DIMM has transcription factor activity in transfected HEK 293 cells and that the PHM gene is a direct target. The mammalian DIMM orthologue MIST1 also transactivated the PHM gene. DIMM activity was dependent on the basic region of the protein and on the sequences of three E-box sites within PHM's first intron; the sites make different contributions to the total activity. These data suggest a model whereby the three E boxes interact cooperatively and independently to produce high PHM transcriptional activation. This DIMM-controlled PHM regulatory region displayed similar properties in vivo. Spatially, its expression mirrored that of the DIMM protein, and its activity was largely dependent on dimm. Further, in vivo expression was highly dependent on the sequences of the same three E boxes. This study supports the hypothesis that DIMM is a master regulator of a peptidergic cell fate in Drosophila and provides a detailed transcriptional mechanism of DIMM action on a defined target gene.
Alteration of Behavioral Changes and Hippocampus Galanin Expression in Chronic Unpredictable Mild Stress-Induced Depression Rats and Effect of Electroacupuncture Treatment

To explore new noninvasive treatment options for depression, this study investigated the effects of electric acupuncture (EA) for depression rat models. Depression in rats was induced by unpredictable chronic mild stress (UCMS) combined with isolation for 21 days. Eighteen male Sprague-Dawley rats were randomly assigned into three groups: control, model, and EA groups. Rats were treated by EA once daily for 21 days. The results showed that body weight and sucrose consumption were significantly increased in EA group than in the model group. The crossing numbers and rearing numbers in the open field test significantly decreased in the model group but not in the EA group. And EA treatments upregulated levels of hippocampus galanin (Gal) in UCMS rats back to relative normal levels. The present study suggested that EA had antidepressant effects on UCMS model rats. The potential antidepressant effect may be related to upregulating Gal expression in hippocampus.
Intelligent integrated information systemfor effective enterprise management: Designing to deployment: An approach

Enterprise management has become extremely complex today. The Internet, information and communication technologies, and compulsions from globalisation, pose both opportunities and threats to the enterprise, of dimensions never seen before. Only the techno-savvy, intelligent, and agile enterprises can manage their business effectively. They seek and depend on intelligent information systems for survival and growth.However, the task of designing, building, and adopting one is quite daunting, given the number and complexities of options.It is in this context that ‘integrated intelligent information system’ [IIIS] assumes importance. Based on literature and experiences published, this paper presents a systematic approach to designing, building and adopting enterprise-appropriate IIIS.
Lignin-Derived Compounds as Efficient Laccase Mediators for Decolorization of Different Types of Recalcitrant Dyes

ABSTRACT Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccases and were compared with those of eight known synthetic mediators (including -NOH- compounds). Among the six types of dyes assayed, only Reactive Blue 38 (phthalocyanine) was resistant to laccase-mediator treatment under the conditions used. Acid Blue 74 (indigoid dye), Reactive Blue 19 (anthraquinoid dye), and Aniline Blue (triarylmethane-type dye) were partially decolorized by the laccases alone, although decolorization was much more efficient and rapid with mediators, whereas Reactive Black 5 (diazo dye) and Azure B (heterocyclic dye) could be decolorized only in the presence of mediators. The efficiency of each natural mediator depended on the type of dye to be treated but, with the only exception being Azure B (<50% decolorization), nearly complete decolorization (80 to 100%) was attained in all cases. Similar rates were attained with the best synthetic mediators, but the reactions were significantly slower. Phenolic aldehydes, ketones, acids, and esters related to the three lignin units were among the best mediators, including p-coumaric acid, vanillin, acetovanillone, methyl vanillate, and above all, syringaldehyde and acetosyringone. The last two compounds are especially promising as ecofriendly (and potentially cheap) mediators for industrial applications since they provided the highest decolorization rates in only 5 to 30 min, depending on the type of dye to be treated.
Retinal ganglion cell-derived sonic hedgehog locally controls proliferation and the timing of RGC development in the embryonic mouse retina

The timing of cell cycle exit and temporal changes in the developmental competence of precursor cells are key components for the establishment of the normal complement of cell types in the mammalian retina. The identity of cell extrinsic cues that control these processes is largely unknown. We showed previously in mouse retina that sonic hedgehog (Shh) signalling from retinal ganglion cells (RGCs) to retinal precursor cells (RPC) is required for the establishment of normal retinal organization. Here, we show that conditional ablation of Shh expression in the peripheral mouse results in a depletion of the RPC pool, owing to precocious cell-cycle exit and neuronal differentiation. These changes were correlated with the downregulation of cyclin D1 and Hes1 gene expression. Shh inactivation also results in an increase in RGC number owing to a bias of RPC towards RGC production. In contrast to zebrafish, where Shh signalling drives cell cycle exit and RGC development, our findings indicate that in the mouse retina Shh signalling is required to maintain RPC proliferation and to control the timing of RGC development.
Coderunner

How should we assess programming skills? Asking students to write code in a traditional hand-written exam can produce results like those in Figure 1. It is nearly impossible to meaningfully grade such code. With sufficient effort one can get some idea of whether the general idea is correct, but to assess programming skill we need much more than this. For example, there will almost certainly be errors in the code; how do we know whether the student would be able to correct those errors or not?
Physiological and psychological responses to a glucose challenge in bulimia

Twenty females meeting DSM-III criteria for bulimia, and 12 age- and sex-matched controls underwent a 60-min intravenous glucose tolerance test. Blood samples were taken at −10, 0, 5, 10, 20, 30, 45, and 60 min after the glucose injection. At these times, subjects completed a mood rating scale [Profile of Mood States (POMS)] and a subjective self-report questionnaire addressing both food cravings and thirst, as well as the urge to binge and subjective control over food intake. Blood levels of tryptophan, tyrosine, valine, leucine, phenylalanine, and isoleucine were measured at 0 and 60 min after the injection. Although equivalent at baseline, the blood glucose levels of bulimics at 5 min and insulin/glucagon ratio at 20, 30, and 45 min following injection were reduced in comparison to controls. Blood valine levels were also lower among the bulimic group. There were no differences between the bulimics and normal controls at baseline on subjective self-report measures of urge to binge, control over food intake, carbohydrate (CHO) craving or fatigue. following the glucose injection, however, the observed physiological differences between the two groups were accompanied by increased subjective cravings for sweets and fruits, an enhanced urge to binge, and less subjective control over food intake for bulimics as compared with controls. The bulimics also reported increased fatigue in response to glucose, whereas controls reported feeling less fatigued. The results suggest that abnormal physiological responses to blood glucose are accompanied by alterations in both food cravings and mood, which may promote binging among bulimic patients.
Putting Sense inside Sensor Systems: A Coordinated Approach to Messaging

Sensor network deployments, such as smart cities and smart logistics, are evolving towards open infrastructure on top of which multiple parties deploy and make use of each other's application software. Of vital importance in such distributed settings, is an agreement on message semantics and contents. Yet, the current state-of-the-art in sensor programming typically remains low-level in terms of messaging, and lacks support for coordination across platforms and stakeholders. We present Talk Sens, a message definition framework that facilitates development of correctly interacting application logic by means of a shared data model, a message description language and serialisation code generation. Additionally, integration of Talk Sens with an existing component model results in run-time retrievable message definitions that facilitate third-party interactions. Our evaluation shows that message sizes, software sizes and middleware overhead remain well within the bounds of sensor network resource constraints.
6. Generating admissible space-time meshes for moving domains in (d + 1) dimensions

In this paper we present a discontinuous Galerkin finite element method for the solution of the transient Stokes equations on moving domains. For the discretization we use an interior penalty Galerkin approach in space, and an upwind technique in time. The method is based on a decomposition of the space-time cylinder into finite elements. Our focus lies on three-dimensional moving geometries, thus we need to triangulate four dimensional objects. For this we will present an algorithm to generate $d+1$-dimensional simplex space-time meshes and we show under natural assumptions that the resulting space-time meshes are admissible. Further we will show how one can generate a four-dimensional object resolving the domain movement. First numerical results for the transient Stokes equations on triangulations generated with the newly developed meshing algorithm are presented.
Efficient storage of transport network routes for simulation models

A heuristic approach is presented which, combined with the use of data structures, reduces storage requirements to store full optimal paths on transportation networks. The goal is to allow the use of full road networks on the implementation of simulations for the operation of a fleet of vehicles. The approach is based on the representation of routing information as a routing matrix, where we exploit structural properties of routing information to reduce storage requirements.
Triggering Respirofermentative Metabolism in the Crabtree-Negative Yeast Pichia guilliermondii by Disrupting the CAT8 Gene

ABSTRACT Pichia guilliermondii is a Crabtree-negative yeast that does not normally exhibit respirofermentative metabolism under aerobic conditions, and methods to trigger this metabolism may have applications for physiological study and industrial applications. In the present study, CAT8, which encodes a putative global transcriptional activator, was disrupted in P. guilliermondii. This yeast's ethanol titer increased by >20-fold compared to the wild type (WT) during aerobic fermentation using glucose. A comparative transcriptional analysis indicated that the expression of genes in the tricarboxylic acid cycle and respiratory chain was repressed in the CAT8-disrupted (ΔCAT8) strain, while the fermentative pathway genes were significantly upregulated. The respiratory activities in the ΔCAT8 strain, indicated by the specific oxygen uptake rate and respiratory state value, decreased to one-half and one-third of the WT values, respectively. In addition, the expression of HAP4, a transcriptional respiratory activator, was significantly repressed in the ΔCAT8 strain. Through disruption of HAP4, the ethanol production of P. guilliermondii was also increased, but the yield and titer were lower than that in the ΔCAT8 strain. A further transcriptional comparison between ΔCAT8 and ΔHAP4 strains suggested a more comprehensive reprogramming function of Cat8 in the central metabolic pathways. These results indicated the important role of CAT8 in regulating the glucose metabolism of P. guilliermondii and that the regulation was partially mediated by repressing HAP4. The strategy proposed here might be applicable to improve the aerobic fermentation capacity of other Crabtree-negative yeasts.
Epistemological and Interdisciplinary Trends in the Continuity of Psychoanalysis

The goal of this volume is to present a ‘cultural genealogy of psychoanalytic developmental psychology’ (p. 3). Kirschner makes the case that post-Freudian psychoanalytic theory is actually a modern, secularized and interiorized recasting of a persistent cultural narrative that she dates back to the earliest days of Christian mysticism. In its basic form, this narrative involves humankind’s union with God (or with what is good), a subsequent falling away, and a striving for re-union. Kirschner traces the reshaping of this core narrative through the neoplatonist philosopher Plotinus, Judeo-Christian theology, the Christian mysticism of Jakob Boehme, and Romantic thought. She contends that the same narrative has been reshaped in each era. Boehme conceived of man’s spiritual development as a ‘spiral’ rather than Plotinus’ ‘circle’, thus bringing a teleology to the narrative, the possibility of development to a ‘higher’ spiritual level. The Romantics stripped away religious content from the narrative, substituting the ‘self’ for ‘God’, focusing on life rather than an afterlife, and conceiving of self-
Automatic Generation of Language-based Tools using LISA system

Many tools have been built in the past years, based on the different formal methods and processing different parts of language specification, such as: scanner generators, parser generators and compiler generators. The automatic generation of a complete compiler was the primary goal of such systems. However, researchers soon recognized the possibility that many other language-based tools could be generated from formal language specifications. In the paper language-based tools generated automatically using LISA system are described. Examples of LISA generated tools among compilers/interpreters are editors, analyzers, and visualizers/animators. In the paper, special emphasis is given to the latter because, to our knowledge, none of the existing compiler generators are able to automatically generate visualizers/animators.