Miracle-dz/UniFAR-FTUs huggingface dataset:
The Effects of Plant-Derived Oleanolic Acid on Selected Parameters of Glucose Homeostasis in a Diet-Induced Pre-Diabetic Rat Model

Prolonged exposure to high energy diets has been implicated in the development of pre-diabetes, a long-lasting condition that precedes type 2 diabetes mellitus (T2DM). A combination of pharmacological and dietary interventions is used to prevent the progression of pre-diabetes to T2DM. However, poor patient compliance leads to negligence of the dietary intervention and thus reduced drug efficiency. Oleanolic acid (OA) has been reported to possess anti-diabetic effects in type 1 diabetic rats. However, the effects of this compound on pre-diabetes have not yet been established. Consequently, this study sought to evaluate the effects OA on a diet-induced pre-diabetes rat model. Pre-diabetic male Sprague Dawley rats were treated with OA in both the presence and absence of dietary intervention for a period of 12 weeks. The administration of OA with and without dietary intervention resulted in significantly improved glucose homeostasis through reduced caloric intake, body weights, plasma ghrelin concentration and glycated haemoglobin by comparison to the pre-diabetic control. These results suggest that OA may be used to manage pre-diabetes as it was able to restore glucose homeostasis and prevented the progression to overt type 2 diabetes.
Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

Piezoelectric biosensors are a group of analytical devices working on a principle of affinity interaction recording. A piezoelectric platform or piezoelectric crystal is a sensor part working on the principle of oscillations change due to a mass bound on the piezoelectric crystal surface. In this review, biosensors having their surface modified with an antibody or antigen, with a molecularly imprinted polymer, with genetic information like single stranded DNA, and biosensors with bound receptors of organic of biochemical origin, are presented and discussed. The mentioned recognition parts are frequently combined with use of nanoparticles and applications in this way are also introduced. An overview of the current literature is given and the methods presented are commented upon.
Origins of heart rate variability: relationship of heart rate burst morphology to work duration and load.

We are developing a lexicon of specific heart period changes, or lexons, that recur frequently and whose physiological meaning can be read into ambulatory electrocardiogram (ECG). The transient, reversible "burst" of tachycardia induced by exercise initiation can also be seen on ambulatory ECG. We hypothesized that burst morphology depended on the work that preceded it and on baroreceptor activation. Ten subjects with mean age 38 yr (range 17-69 yr) underwent two protocols of semisupine cycling in which load and duration were varied. Burst duration increased with longer cycling times (median values of 18.0, 25.5, and 23.7 s with 1, 3, and 5 s of cycling, respectively; P= 0.033). Burst shape as assessed by heart period exponential decay constant and burst magnitude did not change. To assess the impact of workload, subjects cycled for 5 s at loads of 0, 25, 50, and 75 W. No significant differences were seen in burst duration, burst magnitude, or burst shape. Tachycardia preceded hypotension by 4.6 ± 2.2 s, which is inconsistent with baroreceptor involvement in the onset of burst tachycardia. Because burst morphology is a nearly quantal response to the initiation of exercise, the presence of a burst on an ambulatory ECG implies the onset of exercise.
Effects of substance P on human colonic mucosa in vitro.

Previous studies indicated that the peptide substance P (SP) causes Cl--dependent secretion in animal colonic mucosa. We investigated the effects of SP in human colonic mucosa mounted in Ussing chamber. Drugs for pharmacological characterization of SP-induced responses were applied 30 min before SP. Serosal, but not luminal, administration of SP (10-8 to 10-6 M) induced a rapid, monophasic concentration and Cl--dependent, bumetanide-sensitive short-circuit current ( I sc) increase, which was inhibited by the SP neurokinin 1 (NK1)-receptor antagonist CP-96345, the neuronal blocker TTX, the mast cell stabilizer lodoxamide, the histamine 1-receptor antagonist pyrilamine, and the PG synthesis inhibitor indomethacin. SP caused TTX- and lodoxamide-sensitive histamine release from colonic mucosa. Two-photon microscopy revealed NK1(SP)-receptor immunoreactivity on nerve cells. The tyrosine kinase inhibitor genistein concentration dependently blocked SP-induced I sc increase without impairing forskolin- and carbachol-mediated I sc increase. We conclude that SP stimulates Cl--dependent secretion in human colon by a pathway(s) involving mucosal nerves, mast cells, and the mast cell product histamine. Our results also indicate that tyrosine kinases may be involved in this SP-induced response.
Role and New Insights of Pirfenidone in Fibrotic Diseases

Pirfenidone (PFD) is a non-peptide synthetic molecule issued as a broad-spectrum anti-fibrotic drug with the ability to decrease TGF-β1, TNF-α, PDGF and COL1A1 expression, which is highly related to prevent or remove excessive deposition of scar tissue in several organs. Basic and clinical evidence suggests that PFD may safely slow or inhibit the progressive fibrosis swelling after tissue injuries. Furthermore, a number of evidence suggests that this molecule will have positive effects in the treatment of other inflammatory diseases. This review contains current research in which PFD has been used as the treatment of several diseases, and focus mainly in the outcomes related to improve inflammation and fibrogenesis. Therefore, the main goal of this review is to focus on the novel findings of PFD efficacy rather than deepen in the chemical aspects of the molecule.
Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren’s syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure of cells to interact with scaffolds and of the single cell types that are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber scaffolds have been used for in vitro growth of epithelial cells, PLGA has low compliance and cells do not penetrate the scaffolds. Using a core-shell electrospinning technique, we incorporated poly (glycerol sebacate) (PGS) into PLGA scaffolds to increase the compliance and decrease hydrophobicity. PGS/PLGA scaffolds promoted epithelial cell penetration into the scaffold and apical localization of tight junction proteins, which is necessary for epithelial cell function. Additionally, co-culture of the salivary epithelial cells with NIH3T3 mesenchymal cells on PGS/PLGA scaffolds facilitated epithelial tissue reorganization and apical localization of tight junction proteins significantly more than in the absence of the mesenchyme. These data demonstrate the applicability of PGS/PLGA nanofibers for epithelial cell self-organization and facilitation of co-culture cell interactions that promote tissue self-organization in vitro.
Visual equivalence in dynamic scenes

Characterizing the fidelity of an image is a fundamental problem in computer graphics. Visible difference predictors are used to detect when images are perceptually indistinguishable. Visual equivalence introduces a new appearance-based standard for image fidelity, where two visibly different images of an object can still be visually equivalent as representations of the object's appearance if they convey the same shape, material and lighting properties. The original visual equivalence study focused on static scenes. In this paper, our goal is to understand and evaluate visual equivalence in the context of dynamic scenes. We conducted a series of psychophysical experiments that explore visual equivalence for objects undergoing rotational and linear motion under a variety of illumination transformations. We demon- strate that equivalence continues to exist with motion for transformations that maintain some important properties. Specifically, they should not affect the sense of structured motion in the patterns of surface reflections. Based on these insights, we propose a new interpolation transformation that preserves some of these dynamic visual features.
NHE2 contains subdomains in the COOH terminus for growth factor and protein kinase regulation.

The cloned epithelial cell-specific Na+/H+exchanger (NHE) isoform NHE2 is stimulated by fibroblast growth factor (FGF), phorbol 12-myristate 13-acetate (PMA), okadaic acid (OA), and fetal bovine serum (FBS) through a change in maximal velocity of the transporter. In the present study, we used COOH-terminal truncation mutants to delineate specific domains in the COOH terminus of NHE2 that are responsible for growth factor and/or protein kinase regulation. Five truncation mutants (designated by the amino acid number at the truncation site) were stably expressed in NHE-deficient PS120 fibroblasts. The effects of PMA, FGF, OA, FBS, and W-13 [a Ca2+/calmodulin (CaM) inhibitor] were studied. Truncation mutant E2/660, but not E2/573, was stimulated by PMA. OA stimulated E2/573 but not E2/540. FGF stimulated E2/540 but not E2/499. The most truncated mutant, E2/499, was stimulated by FBS. W-13 stimulated the basal activity of the wild-type NHE2. However, W-13 had no effect on E2/755. By monitoring the emission spectra of dansylated CaM fluorescence, we showed that dansylated CaM bound directly to a purified fusion protein of glutathione S-transferase and the last 87 amino acids of NHE2 in a Ca2+-dependent manner, with a stoichiometry of 1:1 and a dissociation constant of 300 nM. Our results showed that the COOH terminus of NHE2 is organized into separate stimulatory and inhibitory growth factor/protein kinase regulatory subdomains. This organization of growth factor/protein kinase regulatory subdomains is very similar to that of NHE3, suggesting that the tertiary structures of the putative COOH termini of NHE2 and NHE3 are very similar despite the minimal amino acid identity in this part of the two proteins.
Voltage-dependent K+ current in capillary endothelial cells isolated from guinea pig heart.

Capillary fragments were isolated from guinea pig hearts, and their electrical properties were studied using the perforated-patch and cell-attached mode of the patch-clamp technique. A voltage-dependent K+ current was discovered that was activated at potentials positive to -20 mV and showed a sigmoid rising phase. For depolarizing voltage steps from -128 to +52 mV, the time to peak was 71 ± 5 ms (mean ± SE) and the amplitude of the current was 3.7 ± 0.5 pA/pF in the presence of 5 mM external K+. The time course of inactivation was exponential with a time constant of 7.2 ± 0.5 s at +52 mV. The current was blocked by tetraethylammonium (inhibitory constant ∼3 mM) but was not affected by charybdotoxin (1 μM) or apamin (1 μM). In the cell-attached mode, depolarization-activated single-channel currents were found that inactivated completely within 30 s; the single-channel conductance was 12.3 ± 2.4 pS. The depolarization-activated K+current described here may play a role in membrane potential oscillations of the endothelium.
Functionalized Gold Nanoparticles for the Detection of C-Reactive Protein

C-reactive protein (CRP) is a very important biomarker of infection and inflammation for a number of diseases. Routine CRP measurements with high sensitivity and reliability are highly relevant to the assessment of states of inflammation and the efficacy of treatment intervention, and require the development of very sensitive, selective, fast, robust and reproducible assays. Gold nanoparticles (Au NPs) are distinguished for their unique electrical and optical properties and the ability to conjugate with biomolecules. Au NP-based probes have attracted considerable attention in the last decade in the analysis of biological samples due to their simplicity, high sensitivity and selectivity. Thus, this article aims to be a critical and constructive analysis of the literature of the last three years regarding the advances made in the development of bioanalytical assays based on gold nanoparticles for the in vitro detection and quantification of C-reactive protein from biological samples. Current methods for Au NP synthesis and the strategies for surface modification aiming at selectivity towards CRP are highlighted.
Norepinephrine-induced Ca2+waves depend on InsP3 and ryanodine receptor activation in vascular myocytes.

In rat portal vein myocytes, Ca2+ signals can be generated by inositol 1,4,5-trisphosphate (InsP3)- and ryanodine-sensitive Ca2+ release channels, which are located on the same intracellular store. Using a laser scanning confocal microscope associated with the patch-clamp technique, we showed that propagated Ca2+ waves evoked by norepinephrine (in the continuous presence of oxodipine) were completely blocked after internal application of an anti-InsP3 receptor antibody. These propagated Ca2+ waves were also reduced by ∼50% and transformed in homogenous Ca2+ responses after application of an anti-ryanodine receptor antibody or ryanodine. All-or-none Ca2+ waves obtained with increasing concentrations of norepinephrine were transformed in a dose-response relationship with a Hill coefficient close to unity after ryanodine receptor inhibition. Similar effects of the ryanodine receptor inhibition were observed on the norepinephrine- and ACh-induced Ca2+ responses in non-voltage-clamped portal vein and duodenal myocytes and on the norepinephrine-induced contraction. Taken together, these results show that ryanodine-sensitive Ca2+release channels are responsible for the fast propagation of Ca2+ responses evoked by various neurotransmitters producing InsP3in vascular and visceral myocytes.
Exploratory Visualization through Copy , Cut and Paste

Our goal is to help oceanographers to visualize and navigate their data over several runs. We have been using parallel coordinate plots to display every data value. Through our copy, cut, paste interactions we aim to enable users to drill-down into specific data points and to explore the datasets in a more expressive way. The method allows users to manipulate the PCP on a ZUI canvas, take copies of the current PCP and paste different subset views.
On Constructing and Visualizing the Topological Structure of the Visibility and Radiance of Architectural Models

The visibility complex organizes the space of maximal segments in the free-space of a 3-D scene into a four-dimensional structure. The cells of the visibility complex have applications in image-based rendering, form-factor computations, global illumination, shadowing and many other methods, but to date only the 0-D and 1-D cells have been constructed. This paper expands the frontier of our understanding of the visibility complex by finding and adding the 2-D cells to the visibility complex. We demonstrate the capabilities of these 2-cells with a rendering application that sparsely samples the illumination of a scene, and interpolates these samples only within the boundaries of the 2-cells, thus avoiding aliasing effects while respecting sharp changes in radiance across boundaries in the visibility.
Rivalry and interference with a head-mounted display

Perceptual factors that affect monocular, transparent (a.k.a "see-thru") head-mounted displays include binocular rivalry, visual interference, and depth of focus. We report the results of an experiment designed to evaluate the effects of these factors on user performance in a table look-up task. Two backgrounds were used. A dynamic moving background was provided by a large screen TV and an untidy bookshelf was used to provide a complex static background. With the TV background large effects were found attributable to both rivalry and visual interference. These two effects were roughly additive. Smaller effects were found with the bookshelf. In conclusion we suggest that monocular transparent HMDs may be unsuitable for use in visually dynamic environments. However when backgrounds are relatively static, having a transparent display may be preferable to having an opaque display.
NATAS: Neural Activity Trace Aware Saliency

Saliency detection has raised much interest in computer vision recently. Many visual saliency models have been developed for individual images, video clips, and image pairs. However, image sequence, one most general occasion in the real world, is not explored yet. A general image sequence is different from video clips whose temporal continuity is maintained and image pairs where common objects exist. It might contain some similar low-level properties while completely distinct contents. Traditional saliency detection methods will fail on these general sequences. Based on this consideration, this paper investigates the shortcomings of the classical saliency detection methods, which significantly limit their advantages: 1) inability to capture the natural connections among sequential images, 2) over-reliance on motion cues, and 3) restriction to image pairs/videos with common objects. In order to address these problems, we propose a framework that performs the following contributions: 1) construct an image data set as benchmark through a rigorously designed behavioral experiment, 2) propose a neural activity trace aware saliency model to capture the general connections among images, and 3) design a novel measure to handle the low-level clues contained among sequential images. Experimental results demonstrate that the proposed saliency model is associated with a tremendous advancement compared with traditional methods when dealing with the general image sequence.
Examining the Motor Phenotype of Patients with Both Essential Tremor and Parkinson's Disease

Background The subset of patients with essential tremor (ET) that develops Parkinson's disease (PD) has not been fully clinically characterized. Methods Motor features were retrospectively reviewed in 18 ET patients who developed PD (ET→PD), 20 ET and 30 PD patients with similar ages and disease durations. Results Fewer ET→PD than ET patients had widespread postural and/or action tremor (2/17 [11.8%] vs. 11/17 [64.7%]; p = 0.001) and marginally fewer had cerebellar signs (1/15 [6.7%] vs. 6/18 [33.3%], p = 0.06). ET→PD patients required fewer ET medications than did their counterparts with ET (p = 0.001). ET→PD patients and PD patients did not differ in UPDRS, Hoehn and Yahr, or Schwab and England scores (each p≥0.14). Discussion ET patients who develop PD may have distinct pre-PD motor features compared to their counterparts with ET who do not develop co-existing PD. Prospective studies are needed to evaluate the predictive value of these clinical features for the emergence of PD.
SMOKE DETECTION USING TEMPORAL HOGHOF DESCRIPTORS AND ENERGY COLOUR STATISTICS FROM VIDEO

We present a novel algorithm for detecting and localizing smoke in video. The first step of our method focuses on detecting the presence of smoke in video frames, while in the second part localization of smoke particles in the scene takes place. In our implementation, we take advantage of both appearance and motion information, so that we can extract robust and meaningful information. Machine learning is used in order to discriminate our data more thoroughly and provide accurate smoke detection. Experiments carried out with various benchmark datasets show that smoke is indeed accurately localized both in time and space via the proposed approach.
Scalable active matching

In matching tasks in computer vision, and particularly in real-time tracking from video, there are generally strong priors available on absolute and relative correspondence locations thanks to motion and scene models. While these priors are often partially used post-hoc to resolve matching consensus in algorithms like RANSAC, it was recently shown that fully integrating them in an ‘Active Matching’ (AM) approach permits efficient guided image processing with rigorous decisions guided by Information Theory. AM's weakness was that the overhead induced by intermediate Bayesian updates required meant poor scaling to cases where many correspondences were sought. In this paper we show that relaxation of the rigid probabilistic model of AM, where every feature measurement directly affects the prediction of every other, permits dramatically more scalable operation without affecting accuracy. We take a general graph-theoretic view of the structure of prior information in matching to sparsify and approximate the interconnections. We demonstrate the performance of two variations, CLAM and SubAM, in the context of sequential camera tracking. These algorithms are highly competitive with other techniques at matching hundreds of features per frame while retaining great intuitive appeal and the full probabilistic capability to digest prior information.
The Cost of Teacher Turnover in Five School Districts: A Pilot Study.

In this paper, we report the results of a pilot study of the cost of teacher turnover in five school districts. We examine the rate of turnover, the relationship between turnover and teacher and school characteristics, and the costs associated with recruiting, hiring, and training replacement teachers. We find evidence that turnover costs, although difficult to quantify, are significant at both the district and the school level. We also find that teachers left high minority and low performing schools at significantly higher rates. This has implications for the differential impact of the costs of teacher turnover on high-need schools. The relationship between teacher turnover and other school and teacher characteristics varied across the five school districts.