Miracle-dz/UniFAR-FTUs huggingface dataset:
Covalent Binding of the Nitroso Metabolite of Sulfamethoxazole Is Important in Induction of Drug-Specific T-Cell Responses in Vivo

Immune-mediated drug hypersensitivity reactions (IDHRs) represent a significant problem due to their unpredictable and severe nature, as well as the lack of understanding of the pathogenesis. Sulfamethoxazole (SMX), a widely used antibiotic, has been used as a model compound to investigate the underlying mechanism of IDHRs because it has been associated with a relatively high incidence of hypersensitivity. Previous studies by others showed that administration of 4-(nitroso)-N-(5-methyl-1,2-oxazol-3-yl)benzenesulfonamide (SMX-NO), the reactive metabolite of SMX, to rats resulted in the generation of SMX-specific antibodies and ex vivo splenocyte proliferative responses, as well as haptenation of skin keratinocytes, circulating peripheral blood mononuclear cells, and splenocytes. The objective of the present study was to further investigate SMX-NO-protein binding in relationship to its immunogenicity. In female DBA/1 mice treated with SMX-NO, varying degrees of SMX-NO-dependent T-cell responses and SMX-NO-protein adduct formation were observed in the spleen and in inguinal, brachial, and axillary lymph nodes. The data suggested a tissue-specific threshold of SMX-NO dosage that triggers the detection of adducts and immune response. Furthermore, serum albumin and immunoglobulin were identified as protein targets for SMX-NO modification. It seemed that these adducts were formed in the blood, circulated to lymphoid tissues, and initiated SMX-NO-dependent immune responses. Collectively, these data revealed a causal link between the deposition of SMX-NO-protein adducts in a lymphoid tissue and the induction of immune response in that tissue. Our findings also suggest that the immunogenicity of SMX-NO is determined by the immunogenic nature of the hapten, rather than special characteristics of the adducted protein.
Duplication of pilus gene complexes of Haemophilus influenzae biogroup aegyptius

Brazilian purpuric fever (BPF) is a recently described pediatric septicemia caused by a strain of Haemophilus influenzae biogroup aegyptius. The pilus specified by this bacterium may be important in BPF pathogenesis, enhancing attachment to host tissue. Here, we report the cloning of two haf (for H. influenzae biogroup aegyptius fimbriae) gene clusters from a cosmid library of strain F3031. We sequenced a 6.8-kb segment of the haf1 cluster and identified five genes (hafA to hafE). The predicted protein products, HafA to HafD, are 72, 95, 98, and 90% similar, respectively, to HifA to HifD of the closely related H. influenzae type b pilus. Strikingly, the putative pilus adhesion, HifE, shares only 44% identity with HafE, suggesting that the proteins may differ in receptor specificity. Insertion of a mini-gammadelta transposon in the hafE gene eliminated hemadsorption. The nucleotide sequences of the haf1 and haf2 clusters are more than 99% identical. Using the recently published sequence of the H. influenzae Rd genome, we determined that the haf1 complex lies at a unique position in the chromosome between the pmbA gene and a hypothetical open reading frame, HI1153. The location of the haf2 cluster, inserted between the purE and pepN genes, is analogous to the hif genes on H. influenzae type b. BPF fimbrial phase switching appears to involve slip-strand mispairing of repeated dinucleotides in the pilus promoter. The BPF-associated H. influenzae biogroup aegyptius pilus system generally resembles other H. influenzae, but the possession of a second fimbrial gene cluster, which appears to have arisen by a recent duplication event, and the novel sequence of the HafE adhesin may be significant in the unusual pathogenesis of BPF.
An Optimal Geometric Model for Clavels Delta Robot

This paper discusses the Clavel's Delta parallelrobot and proposes an alternate solution to its kinematics/dynamic model. We meant to integrate these models into ona small electrical driving circuit that integrates an onboard microcontroller.We designed the solution by taking into accountthe reduced computing capability of small embedded systems.Direct kinematics (DK), differential kinematics, both direct (J)and inverse (invJ), and a simplified dynamic model will alsobe presented. The novelty of the approach relies in a series ofgeometric properties that allow to reduce the computationalload. When the three kinematics are computed together (DK,J, invJ), their computations can be expressed in few lines ofcode. The accuracy of motion, as well as the reduced computingpower, will be compared to classic algorithms . The proposedalgorithms have been implemented in a working system in thecontext of a telemedicine project.
Adaptation to ER Stress Is Mediated by Differential Stabilities of Pro-Survival and Pro-Apoptotic mRNAs and Proteins

The accumulation of unfolded proteins in the endoplasmic reticulum (ER) activates a signaling cascade known as the unfolded protein response (UPR). Although activation of the UPR is well described, there is little sense of how the response, which initiates both apoptotic and adaptive pathways, can selectively allow for adaptation. Here we describe the reconstitution of an adaptive ER stress response in a cell culture system. Monitoring the activation and maintenance of representative UPR gene expression pathways that facilitate either adaptation or apoptosis, we demonstrate that mild ER stress activates all UPR sensors. However, survival is favored during mild stress as a consequence of the intrinsic instabilities of mRNAs and proteins that promote apoptosis compared to those that facilitate protein folding and adaptation. As a consequence, the expression of apoptotic proteins is short-lived as cells adapt to stress. We provide evidence that the selective persistence of ER chaperone expression is also applicable to at least one instance of genetic ER stress. This work provides new insight into how a stress response pathway can be structured to allow cells to avert death as they adapt. It underscores the contribution of posttranscriptional and posttranslational mechanisms in influencing this outcome.
Punctuation and Implicit Prosody in Silent Reading: An ERP Study Investigating English Garden-Path Sentences

This study presents the first two ERP reading studies of comma-induced effects of covert (implicit) prosody on syntactic parsing decisions in English. The first experiment used a balanced 2 × 2 design in which the presence/absence of commas determined plausibility (e.g., John, said Mary, was the nicest boy at the party vs. John said Mary was the nicest boy at the party). The second reading experiment replicated a previous auditory study investigating the role of overt prosodic boundaries in closure ambiguities (Pauker et al., 2011). In both experiments, commas reliably elicited CPS components and generally played a dominant role in determining parsing decisions in the face of input ambiguity. The combined set of findings provides further evidence supporting the claim that mechanisms subserving speech processing play an active role during silent reading.
Fuzzy Cognitive Maps for Medical Decision Support — A paradigm from obstetrics

Medical Decision Support Systems can provide assistance in crucial clinical judgments, particularly for inexperienced medical professionals. Fuzzy Cognitive Maps (FCMs) is a soft computing technique for modeling complex systems following an approach similar to human reasoning and decision-making. FCMs successfully represent knowledge and human experience, introducing concepts to represent the essential elements and the cause and effect relationships among the concepts to model the behavior of any system. Medical Decision Systems are complex systems that can be decomposed to subsystems and elements, where many factors have to be taken into consideration that may be complementary, contradictory, and competitive; these factors influence each other and determine the overall clinical decision with varying degrees. Here a Medical Decision Support System based on an appropriate FCM architecture is proposed and developed, as well as a corresponding paradigm from obstetrics is described.
BLU: Blue-printing Interference for Robust LTE Access in Unlicensed Spectrum

Deploying LTE networks in unlicensed spectrum requires us to move beyond coexistence mechanisms and understand the suitability of LTE's synchronous operation in a spectrum that is governed by asynchronous access principles. Our study reveals a fundamental conflict in LTE uplink access that arises between the scheduled nature of LTE's multi-user transmissions -- critical for leveraging the diversity (OFDMA) and multiplexing (multi-user MIMO) gains -- and the asynchronous nature of interference on the clients. The result is a significant loss in spectrum utilization and throughput that scales with the number of interfering terminals. To tackle this critical challenge on the LTE uplink, we propose Blu. Blu transforms today's LTE schedulers into speculative schedulers that leverage interference diversity across clients to intelligently over-schedule clients on the same spectral resources to prevent this utilization loss. Blu's challenges lie in how to over-schedule appropriate clients on the same resources without paying the penalty of collisions, while containing the exponential overhead incurred in measuring the required interference dependencies between clients. The under-pinning of Blu's design includes a novel mechanism to blue-print the very source of interference on LTE clients along with their dependencies, which allows for a constant, significantly reduced overhead. Blu can be realized in today's LTE base stations. Its realization in an enterprise environment with SDRs (hosting LTE release 10) reveals appreciable gains of 1.5-2x in both utilization and throughput over existing schemes for SISO and MU-MIMO transmissions in unlicensed spectrum.
The road back.

Rating: Teen And Up Audiences Archive Warning: Choose Not To Use Archive Warnings Category: F/M Fandom: The 100 (TV) Relationship: Bellamy Blake/Clarke Griffin Character: Clarke Griffin, Bellamy Blake, Raven Reyes, Finn Collins, Octavia Blake, Jasper Jordan, Lincoln (The 100), Anya (The 100) Additional Tags: Alternate Universe Stats: Published: 2014-06-02 Completed: 2014-07-13 Chapters: 29/29 Words: 65388
A Human Immunodeficiency Virus Protease Inhibitor Is a Novel Functional Inhibitor of Human Pregnane X Receptor

Drug-drug interactions involving induction of cytochrome P450 enzymes (P450s) can lead to loss of drug efficacy. Certain drugs, particularly those used to treat mycobacterial and human immunodeficiency virus (HIV) infections, are especially prone to induce P450s. During studies to examine drug-interaction potential of compounds in cultured human hepatocytes, exposure with (S)-1-[(1S,3S,4S)-4-[(S)-2-(3-benzyl-2-oxo-imidazolidin-1-yl)-3,3-dimethyl-butyrylamino]-3-hydroxy-5-phenyl-1-(4-pyridin-2-yl-benzyl)-pentylcarbamoyl]-2,2-dimethyl-propyl-carbamic acid methyl ester (A-792611), a novel HIV protease inhibitor (PI) previously under investigation for the treatment of HIV infection, resulted in significant down-regulation of constitutive CYP3A4 expression. Furthermore, coadministration of A-792611 was found to attenuate CYP3A4 induction mediated by known inducers rifampin and efavirenz. A-792611 also attenuated the rifampin and ritonavir-mediated activation of the human pregnane X receptor (PXR) in luciferase reporter assays. Microarray analysis on cultured human hepatocytes revealed that A-792611 treatment down-regulated the expression of PXR target genes CYP3A4, CYP2B6, CYP2C8, and CYP2C9, whereas there was a lack of inductive effect observed in treated rat hepatocytes. A-792611 did not interact with other ligand-activated nuclear receptors that regulate P450 expression such as constitutive androstane receptor, farnesoid X receptor, vitamin D receptor, and peroxisome proliferator-activated receptor α. These data suggest that A-792611 is a functional and effective human PXR inhibitor. Among the class of HIV-PIs, which are typically PXR activators, A-792611 seems to have a unique property for PXR antagonism and could be a useful tool for studying nuclear receptor pathway regulation.
Decision Directed Channel Estimation Aided OFDM Employing Sample-Spaced and Fractionally-Spaced CIR Estimators

In this letter we characterize the substantial difference between two channel estimation approaches, namely the sample-spaced (SS) and the fractionally-spaced (FS) channel impulse response (CIR) estimators. The achievable performance of decision-directed channel estimation (DDCE) methods employing both the SS- and the FS-CIR estimators is analyzed in the context of an OFDM system. The performance of the two estimation methods is compared and it is shown that the DDCE scheme employing the projection approximation subspace tracking (PAST)-aided FS-CIR estimator outperforms its SS-CIR estimator-based counterpart
The Significance of Rhinologic Evaluation and Pretreatment for Patients Undergoing Two-Jaw Surgery Through Nasotracheal Intubation

AbstractThe aim of this study is to determine whether rhinologic preoperative evaluation and pretreatments reduce intranasal trauma and decrease postoperative complications, such as nasal obstruction and epistaxis, in patients undergoing 2-jaw surgery with nasotracheal intubation. This study included 360 patients with malocclusion (Class III) who underwent 2-jaw surgery under general anesthesia via nasotracheal intubation in our hospital from January to December 2013 and categorized into 3 groups. Nasotracheal intubation was performed according to the nasal cavity the patient was able to breathe comfortably (Group I). The site of nasotracheal intubation was decided by 1 rhinologic specialist who evaluated preoperative dental computed tomography (Group II). The site of nasotracheal intubation was decided upon nasal endoscopic findings, dental computed tomography evaluation, and rhinologic pretreatment (Group III). Group II and Group III showed less damage to the nasal mucosa compared with the nasal status of Group I. Upon comparing Group II and Group III, Group III showed better overall status of the nasal mucosa compared with Group II. Visual analogue scale scores for nasal obstruction were pretty similar for all groups on the first postoperative day. In Group III, the nasal mucosa, however, was improved to that of preoperative status on the third postoperative day.In conclusion, it may be useful to pre-evaluate the mucosal and anatomical status of the nasal cavity to select patients requiring rhinologic pretreatment and decide the site for nasotracheal intubation to minimize complications arising from nasotracheal intubation.
Influence of AlGaN Buffer Growth Temperature on GaN Epilayer based on Si(lll) Substrate

This paper investigated the influence of AlGaN buffer growth temperature on strain status and crystal quality of the GaN film on Si(111) sbustrates by metal organic chemical vapor deposition. It was demonstrated by the optical microscopy that AlGaN buffer gorwth temperature had a remarkable effect on compensating tensil stress in top GaN layer and preventing the formation of cracks. X-ray diffraction and atomic force microscopy analysis showed crystal quality and surface morphology of the GaN epilayer could be improved through increasing AlGaN buffer growth temperature. 1μm crack-free GaN epilayer on Si (111) substrates was obtained with graded AlGaN buffer layer at optimized temperature of 1050°C. Transmission electron microscopy analysis revealed that a significant reduction in threading dislocations was achieved in GaN epilayer.
Plasma Adiponectin Does Not Correlate With Insulin Resistance and Cardiometabolic Variables in Nondiabetic Asian Indian Teenagers

OBJECTIVE—The objectives of this study were to determine age- and sex-specific concentrations of adiponectin in Asian Indian teenagers and adults and to assess whether its blood levels correlated with insulin resistance and other cardiometabolic parameters. RESEARCH DESIGN AND METHODS—We studied 196 teenagers (94 boys, 102 girls) 12–18 years of age, selected from a cohort of 2,640 individuals from a cross-sectional school-based survey in Chennai, India. For comparison, adiponectin and plasma insulin were measured in 84 healthy adults. Correlation of adiponectin with plasma levels of insulin, proinsulin, insulin resistance, anthropometry, and family history of diabetes were studied. RESULTS—Adiponectin showed a sex dimorphism, with girls having higher values (in μg/ml) (10.3 ± 5.0) than boys (8.4 ± 3.5) (P < 0.0001), and it showed a positive correlation with HDL cholesterol in boys only and not with other lipid parameters, insulin resistance, proinsulin, anthropometry, and family history of diabetes. In the adults, adiponectin correlated with fasting glucose and inversely with triglycerides. CONCLUSIONS—In Asian Indian adults and teenagers, adiponectin did not correlate directly with measures of insulin sensitivity, overweight, and other cardiometabolic variables. This was at variance with several reports in other populations showing an inverse association of adiponectin with insulin resistance, proinsulin, and BMI, suggesting ethnic differences in the relationship of adiponectin with insulin sensitivity. The role of adiponectin in relation to action of insulin needs more detailed studies in Asian Indians.
AppHolmes: Detecting and Characterizing App Collusion among Third-Party Android Markets

Background activities on smartphones are essential to today's "always-on" mobile device experience. Yet, there lacks a clear understanding of the cooperative behaviors among background activities as well as a quantification of the consequences. In this paper, we present the first in-depth study of app collusion, in which one app surreptitiously launches others in the background without user's awareness. To enable the study, we develop AppHolmes, a static analysis tool for detecting app collusion by examining the app binaries. By analyzing 10,000 apps from top third-party app markets, we found that i) covert, cooperative behaviors in background app launch are surprisingly pervasive, ii) most collusion is caused by shared services, libraries, or common interest among apps, and iii) collusion has serious impact on performance, efficiency, and security. Overall, our work presents a strong implication on future mobile system design.
EXIT CHART ANALYSIS APPLIED TO ADAPTIVE TURBO EQUALIZATION

In this paper we consider iterative channel estimation, equalization, and decoding, or adaptive Turbo equalization, as a receiver technology for digital communication systems where the channel imposes time-varying intersymbol interference. We show how a semianalytical technique called EXIT charts, originally developed by S. ten Brink for analysis of Turbo codes, can be used to predict the performance of the iterative receiver for such systems. To demonstrate the usefulness of the technique, we use EXIT charts to address interesting questions about adaptive Turbo equalization for time-varying channels: Which pattern of training sequences should be used, what is the performance difference between optimal MAP equalization and suboptimal linear equalization, and between the case of a known channel and the case of an estimated channel, and what can be gained by using a recursive precoder in conjunction with the symbol mapper.
Susceptibility to tuberculosis is associated with variants in the ASAP1 gene encoding a regulator of dendritic cell migration

Human genetic factors predispose to tuberculosis (TB). We studied 7.6 million genetic variants in 5,530 people with pulmonary TB and in 5,607 healthy controls. In the combined analysis of these subjects and the follow-up cohort (15,087 TB patients and controls altogether), we found an association between TB and variants located in introns of the ASAP1 gene on chromosome 8q24 (P = 2.6 × 10−11 for rs4733781; P = 1.0 × 10−10 for rs10956514). Dendritic cells (DCs) showed high ASAP1 expression that was reduced after Mycobacterium tuberculosis infection, and rs10956514 was associated with the level of reduction of ASAP1 expression. The ASAP1 protein is involved in actin and membrane remodeling and has been associated with podosomes. The ASAP1-depleted DCs showed impaired matrix degradation and migration. Therefore, genetically determined excessive reduction of ASAP1 expression in M. tuberculosis–infected DCs may lead to their impaired migration, suggesting a potential mechanism of predisposition to TB.
Elaboration of the preliminary Rheumatoid Arthritis Impact of Disease (RAID) score: a EULAR initiative

Background: Current response criteria in rheumatoid arthritis (RA) usually assess only three patient-reported outcomes (PROs): pain, functional disability and patient global assessment. Other important PROs such as fatigue are not included. Objective: To elaborate a patient-derived composite response index for use in clinical trials in RA, the RA Impact of Disease (RAID) score. Methods: Ten patients identified 17 domains or areas of health relevant for inclusion in the score, then 96 patients (10 per country in 10 European countries) ranked these domains in order of decreasing importance. The seven most important domains were selected. Instruments were chosen for each domain after extensive literature research of psychometric properties and expert opinion. The relative weight of each of the domains was obtained from 505 patients who were asked to “distribute 100 points” among the seven domains. The average ranks of importance of these domains were then computed. Results: The RAID score includes seven domains with the following relative weights: pain (21%), functional disability (16%), fatigue (15%), emotional well-being (12%), sleep (12%), coping (12%) and physical well-being (12%). Weights were similar across countries and across patient and disease characteristics. Proposed instruments include the Health Assessment Questionnaire and numerical ratings scales. Conclusion: The preliminary RAID score is a patient-derived weighted score to assess the impact of RA. An ongoing study will allow the final choice of questionnaires and assessment of validity. This score can be used in clinical trials as a new composite index that captures information relevant to patients.
Mining periodic patterns with gap requirement from sequences

We study a problem of mining frequently occurring periodic patterns with a gap requirement from sequences. Given a character sequence S of length L and a pattern P of length l, we consider P a frequently occurring pattern in S if the probability of observing P given a randomly picked length-l subsequence of S exceeds a certain threshold. In many applications, particularly those related to bioinformatics, interesting patterns are periodic with a gap requirement. That is to say, the characters in P should match subsequences of S in such a way that the matching characters in S are separated by gaps of more or less the same size. We show the complexity of the mining problem and discuss why traditional mining algorithms are computationally infeasible. We propose practical algorithms for solving the problem, and study their characteristics. We also present a case study in which we apply our algorithms on some DNA sequences. We discuss some interesting patterns obtained from the case study.
DCT-compressive sampling of multifrequency sparse audio signals

In this paper, we propose to apply the discrete cosine transform (DCT) and the compressive sampling (CS) techniques to audio signal compression. Using spectral analysis and the properties of the DCT, we can treat audio signals as sparse signals in the frequency domain. This is especially true for sounds representing tones. Thus, we propose the use of DCT as a preprocessor in order to sparsely represent a signal in the frequency domain, combined with CS to obtain an efficient representation of the signals. We show that the subsequent application of CS represents our signals with less information than the well-known sampling theorem.