Miracle-dz/UniFAR-FTUs huggingface dataset:
A Rho-associated protein kinase: differentially distributed in limbal and corneal epithelia.

PURPOSE The authors have developed monoclonal antibodies (mAbs) to characterize the sequential biochemical changes in corneal epithelial cells after they differentiate from stem cells, located in the limbus, and migrate centripetally to follow the pathway of terminal differentiation. The purpose of this study was to identify a protein (recognized by mAb HE1/11F) with increased expression associated with the transition of the limbal epithelium to corneal epithelium. METHODS The distribution and identification of the protein(s) were performed using an indirect immunohistochemical staining technique and a western blot analysis, respectively. A rabbit corneal epithelial cDNA library, constructed in the Uni-Zap XR vector, was screened with mAb HE1/11F to select cDNA clones expressing polypeptide(s) recognized by this mAb. Additional overlapping cDNA clones were obtained from a primer extension cDNA library to determine the sequence of the complete open reading frame encoding the protein recognized by mAb HE1/11F. RESULTS Rabbit corneal epithelium exhibited strong immunostaining with mAb HE1/11F, however, the limbal epithelial cells stained weakly. HE1/11F recognized 160-kDa (HEBM1) and 100-kDa (HEBM2) polypeptides in the corneal epithelial extracts. The amino acid sequence of the protein deduced from the nucleotide sequence of the cDNA exhibited a close homology to that of a RhoA (Ras-related small GTPase)-associated serine-threonine kinase (ROCK-I or Rho-associated coiled-coil kinase). A 160-kDa RhoA-binding polypeptide with a molecular mass similar to that of HEBM1 and ROCK-I was detected in the corneal epithelial extracts. These findings strongly suggested that HEBM1 was rabbit ROCK-I. The identity of HEBM1 was further confirmed from the reactivity of mAb HE1/11F with ROCK-I immunoprecipitated from rabbit corneal epithelial extracts using anti-ROCK-I antibodies. CONCLUSIONS The increased expression of a protein identified as ROCK-I from cDNA analyses is associated with rabbit corneal epithelial differentiation and transition from the limbal to corneal surface. Therefore, a RhoA signaling pathway is likely to be associated with corneal epithelial differentiation (maturation). A close homology among the cDNA sequences of rabbit, mouse, rat, and human ROCK-I indicates that this RhoA-associated kinase is a well-conserved protein.
Integrin beta 1- and beta 3-mediated endothelial cell migration is triggered through distinct signaling mechanisms

Human umbilical vein endothelial cell attachment, spreading and migration on collagen and vitronectin are mediated by integrins alpha 2 beta 1 and alpha v beta 3, respectively, and these events take place in the absence of cytokines, growth factors, or chemoattractants. Cell attachment and spreading on these ligands occur in the absence of extracellular calcium, as does migration on collagen. In contrast, vitronectin-mediated migration is absolutely dependent on the presence of extracellular calcium. Cell contact with immobilized vitronectin or anti-alpha v beta 3 mAbs promotes a measurable rise in [Ca2+]i which requires an extracellular calcium source, whereas collagen, or anti- alpha 2 beta 1 mAbs fail to promote this signaling event. In fact, vitronectin-mediated migration and the rise in intracellular calcium showed the same dose dependence on extracellular calcium. While vitronectin and collagen differ in their ability to induce a calcium influx both ligands or antibodies to their respective integrins promote an equivalent increase in intracellular pH consistent with activation of the Na/H antiporter an event independent of extracellular calcium. These results support two salient conclusions. Firstly, collagen and vitronectin, through their respective integrins, promote distinct intracellular signaling events. Secondly, the alpha v beta 3 specific influx of calcium is not required for cell spreading yet appears to facilitate cellular migration on vitronectin.
Robust Concentration and Frequency Control in Oscillatory Homeostats

Homeostatic and adaptive control mechanisms are essential for keeping organisms structurally and functionally stable. Integral feedback is a control theoretic concept which has long been known to keep a controlled variable robustly (i.e. perturbation-independent) at a given set-point by feeding the integrated error back into the process that generates . The classical concept of homeostasis as robust regulation within narrow limits is often considered as unsatisfactory and even incompatible with many biological systems which show sustained oscillations, such as circadian rhythms and oscillatory calcium signaling. Nevertheless, there are many similarities between the biological processes which participate in oscillatory mechanisms and classical homeostatic (non-oscillatory) mechanisms. We have investigated whether biological oscillators can show robust homeostatic and adaptive behaviors, and this paper is an attempt to extend the homeostatic concept to include oscillatory conditions. Based on our previously published kinetic conditions on how to generate biochemical models with robust homeostasis we found two properties, which appear to be of general interest concerning oscillatory and homeostatic controlled biological systems. The first one is the ability of these oscillators (“oscillatory homeostats”) to keep the average level of a controlled variable at a defined set-point by involving compensatory changes in frequency and/or amplitude. The second property is the ability to keep the period/frequency of the oscillator tuned within a certain well-defined range. In this paper we highlight mechanisms that lead to these two properties. The biological applications of these findings are discussed using three examples, the homeostatic aspects during oscillatory calcium and p53 signaling, and the involvement of circadian rhythms in homeostatic regulation.
Another Look at the Transactions Demand for Money in Nigeria

This study sets out to model non-bank public’s desired holdings of five different measures of money in the Nigerian economy. These are currency outside banks (COB), demand deposits (DD), narrow money (M1), quasi money (QM), and broad money (M2). The study addresses many of the pitfalls involved in the empirical modelling of the well established theory on transaction demand for money with data from an economic setting where there is no consensus in the literature on what constitutes ‘relevant background information’ that must guide the choice of empirical proxies for the variables suggested by theory.The study also explores alternative approaches for dealing with these choices and demonstrates the sensitivity of the modelling outcomes to the different empirical specifications and econometric estimators. It found that some of the approaches suggested in extant literature on Nigeria lead to dead ends, and suggests fruitful alternatives. In particular, it demonstrates the extent to which interest rates could be informative in Nigeria, both in the context of money demand modelling and in the more general context of predicting future economic activity.The study recovers economically sensible and econometrically sound empirical models of long- and short-run demand for only two of the five measures of money: M1 and DD. No meaningful empirical money demand models could be recovered COB, QM and M2. The study identifies rival modelling frameworks that might be fruitfully explored to model COB and M2 in Nigeria, and show some initial empirical results. None of the frameworks seemed capable of explaining QM in Nigeria. Again, it is shown that a possible line of research in the near future is the separate modelling of its two components, savings and time deposits.The study closes with a discussion of the economic insights from the qualitative and quantitative attributes of the long and short run models recovered for M1 and DD, and highlight their implications for monetary policy.
Fault Management Frameworks in Wireless Sensor Networks

With the rapidly gaining ground of the Internet of things, wireless sensor networks (WSNs) will be bound to permeate more and more applications. However, due to the nature of these applications such as harsh environment, resource-limited wireless sensor networks are usually fault prone. Therefore, it is essential to provide effective fault management techniques and robust fault management frameworks for WSNs. This paper makes a comprehensive view of the existing fault management frameworks dedicatedly designed for WSNs along with detail discussions.
InDaQu : Enabling user-centered definition and exchange of consistency constraints for data cleaning ?

Severe data quality problems exist in most public health care systems and inconsistent data sets often occur. Consistency constraints can be used to define valid and invalid data. Existing solutions of such constraints like rule systems are often difficult to maintain, not human-readable, and of a bad quality like containing contradictory rules. With InDaQu we present an approach that allows domain experts to easily create and maintain consistency constraints using an introduced domain-specific language. These constraints are being stored in an ontology, which allows for an automated inconsistency detection in the defined rules themselves. We identified several scenarios in which consistency constraints can be interchanged and exchanged between different participants. The approach has been successfully evaluated in the cancer registry of Lower Saxony.
Energy Emission by Quantum Systems in an Expanding FRW Metric

Bound quantum mechanical systems not expanding with the comoving frame of an expanding, flat FRW metric are found to release energy at a rate linearly proportional to the local Hubble constant ($H_{o}$) and the systems' binding energy ($E_{b}$); {\em i.e.}, $\dot{E} = H_{o} E_{b}$. Three exemplary quantum systems are examined. For systems with early cosmological condensation times | notably hadrons | time-integrated energy release could have been significant and could account for an appreciable fraction of the dark matter inventory.
Growth factors influencing bone development

Summary We have approached the study of growth factors affecting cartilage and bone development by investigating those factors present in bone which are able to initiate new cartilage and bone formation in vivo. This has led to the identification and molecular cloning of seven novel human factors which we have named BMP-1 through BMP-7. Six of these molecules are related to each other, and are also distantly related to TGF-β. The presence of one of these molecules, recombinant human BMP-2 (rhBMP-2) is sufficient to produce the complex developmental system of cartilage and bone formation when implanted subcutaneously in a rat assay system. In this model, administration of rhBMP-2 ultimately results in the formation of a piece of trabecular bone, which is filled with mature bone marrow. While our studies demonstrate that rhBMP-2 by itself has the ability to induce cartilage and bone formation in vivo, we find other BMP molecules present along with BMP-2 in our highly purified nonrecombinant bone-inductive material. These results suggest that the bone inductive capacity of bone-derived proteins may reside in the combinatorial or synergistic activities of this set of BMP-2 related molecules.
Probing the bulk ionic conductivity by thin film hetero-epitaxial engineering

Abstract Highly textured thin films with small grain boundary regions can be used as model systems to directly measure the bulk conductivity of oxygen ion conducting oxides. Ionic conducting thin films and epitaxial heterostructures are also widely used to probe the effect of strain on the oxygen ion migration in oxide materials. For the purpose of these investigations a good lattice matching between the film and the substrate is required to promote the ordered film growth. Moreover, the substrate should be a good electrical insulator at high temperature to allow a reliable electrical characterization of the deposited film. Here we report the fabrication of an epitaxial heterostructure made with a double buffer layer of BaZrO3 and SrTiO3 grown on MgO substrates that fulfills both requirements. Based on such template platform, highly ordered (001) epitaxially oriented thin films of 15% Sm-doped CeO2 and 8 mol% Y2O3 stabilized ZrO2 are grown. Bulk conductivities as well as activation energies are measured for both materials, confirming the success of the approach. The reported insulating template platform promises potential application also for the electrical characterization of other novel electrolyte materials that still need a thorough understanding of their ionic conductivity.
Characterization of angiotensin II receptor subtypes in rat heart.

Angiotensin II exerts positive inotropic and chronotropic effects on the mammalian heart by binding to specific membrane receptors. Recently, two subtypes of angiotensin II receptors (AT1 and AT2) have been distinguished by using the nonpeptide antagonists losartan (previously known as DuP 753) and PD123177. To evaluate the tissue distribution and subtypes of angiotensin II receptors in rat heart, we performed a 125I-[Sar1,Ile8]angiotensin II in situ binding assay on tissue sections obtained from adult Sprague-Dawley rats (10 and 14 weeks old). Binding specificity was verified by competition with unlabeled [Sar1]angiotensin II. Distribution of AT1 and AT2 receptors was determined by competition with losartan and PD123177, respectively, and the density of the receptors was quantified by emulsion autoradiography. Angiotensin II receptors were widely distributed throughout the heart, with each receptor subtype accounting for approximately 50% of the specific binding. Binding density was comparable in the atria, right and left ventricles, intraventricular septum, and sinoatrial node, whereas it was significantly greater in the atrioventricular node. The AT1 receptor appears to interact with guanidine nucleotide regulatory proteins, because GTP-gamma-S causes dissociation of the radioligand from this receptor. In contrast, the AT2 receptor does not appear to directly interact with guanine nucleotide regulatory proteins, inasmuch as radioligand dissociation from this receptor subtype is not affected by GTP-gamma-S. Because angiotensin II has been reported to have growth-potentiating effects in several tissues, we examined angiotensin II receptors in fetal (embryonic days 16 and 19) and neonatal (1-, 2-, 3-, and 10-day-old) rats. (ABSTRACT TRUNCATED AT 250 WORDS)
Brood size, sibling competition and the cost of begging in great tits (Parus major)

Evolutionary theory of parent-offspring conflict explains begging displays of nestling birds as selfish attempts to influence parental food allocation. Models predict that this conflict may be resolved by honest signaling of offspring need to parents, or by competition among nestmates, leading to escalated begging scrambles. Although the former type of models has been qualitatively supported by experimental studies, the potential for a begging component driven by scramble competition cannot be excluded by the evidence. In a brood-size manipulation experiment with great tits, Parus major, we explored the scramble component in the begging activity of great tit nestlings by investigating the mechanisms of sibling competition in relation to brood size. While under full parental compensation, the feeding rate per nestling will remain constant over all brood sizes for both types of models; the scramble begging models alone predict an increase in begging intensity with brood size, if begging costs do not arise exclusively through predation. Great tit parents adjusted feeding rates to brood size and fed nestlings at similar rates and with similar prey sizes in all three brood-size categories. Despite full parental compensation, the begging and food solicitation activities increased with experimental brood size, whereas nestling body condition deteriorated. These findings support a scramble component in begging and suggest that the competition-induced costs of food solicitation behavior play an important role in the evolution of parent-offspring communication. Copyright 2003.
What technique should I use to measure cardiac output?

Purpose of reviewSeveral less invasive cardiac output monitoring techniques are now commercially available and have the potential to replace the pulmonary artery catheter under certain clinical circumstances. The aim of this review is to give a synopsis of the currently available cardiac output measurement methods. This information should help in selecting the appropriate technique in a particular clinical setting. Recent findingsAn overview is given of the currently available techniques for cardiac output monitoring. Recent validation studies demonstrate that pulse wave analysis may be used reliably as an alternative to the pulmonary artery catheter in different clinical settings. The use of transesophageal echocardiography and Doppler measurements is limited due to high operator dependency, the partial carbon dioxide rebreathing technique should be applied in a precisely defined clinical setting to mechanically ventilated patients only, and pulsed dye densitometry as well as the bioimpedance technique are currently primarily applied in an investigational setting. SummaryLess invasive cardiac output monitoring techniques may replace the pulmonary artery catheter in different clinical settings considering the specific properties of these techniques. The pulmonary artery catheter, however, may still be recommended for cardiac output measurement in specific clinical situations when monitoring of pulmonary artery pressures is desirable.
Business Cycles and Exchange Rate Regimes

Are business cycles different according to the exchange rate regime? Baxter & Stockman (1989) have inconclusive results. We reinvestigate this question with more recent data and a different methodology and find evidence, for some aggregates, that their business cycle behavior has changed. We then try to replicate this change with an international real business cycle model with exchange rate shocks. Les cycles economiques sont-ils differents selon le regime de taux de change? Baxter & Stockman (1989) n'obtiennent pas de reponse definitive a cette question. Nous la reexaminons avec des donnees plus recentes et une methodologie differente et trouvons qu'il y a eu changement, du moins pour certains agregats economiques. Ensuite, nous cherchons a repliquer ce fait au moyen d'un modele du cycle reel international augmente de chocs exogenes sur le taux de change reel.
Multipotent Mesenchymal Stem Cell Treatment for Discogenic Low Back Pain and Disc Degeneration

Low back pain with resultant loss of function, decreased productivity, and high economic costs is burdensome for both the individual and the society. Evidence suggests that intervertebral disc pathology is a major contributor to spine-related pain and degeneration. When commonly used conservative therapies fail, traditional percutaneous or surgical options may be beneficial for pain relief but are suboptimal because of their inability to alter disc microenvironment catabolism, restore disc tissue, and/or preserve native spine biomechanics. Percutaneously injected Multipotent Mesenchymal Stem Cell (MSC) therapy has recently gained clinical interest for its potential to revolutionarily treat disc-generated (discogenic) pain and associated disc degeneration. Unlike previous therapies to date, MSCs may uniquely offer the ability to improve discogenic pain and provide more sustained improvement by reducing disc microenvironment catabolism and regenerating disc tissue. Consistent treatment success has the potential to create a paradigm shift with regards to the treatment of discogenic pain and disc degeneration.
Techniques for Leakage Power Reduction in Nanoscale Circuits : A Survey 1

This report surveys progress in the field of designing low power especially low leakage CMOS circuits in deep submicron era. The leakage mechanism and various recently proposed run time leakage reduction techniques are presented. Two designs from Cadence and Sony respectively, which can represent current industrial application of these techniques, are also illustrated. 1 The work behind this report is carried out as a 13-week special course with associate professor Alberto Nannarelli as supervisor during the autumn semester 2006 at the Department of Informatics and Mathematical Modeling, Technical University of Denmark.
Identification of Immunodominant CD4-Restricted Epitopes Co-Located with Antibody Binding Sites in Individuals Vaccinated with ALVAC-HIV and AIDSVAX B/E

We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.
Long-term persistence of molecular disease after histological remission in low-grade gastric MALT lymphoma treated with H. pylori eradication. Lack of association with translocation t(11;18): a 10-year updated follow-up of a prospective study.

BACKGROUND Localized low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma can regress after Helicobacter pylori eradication, but IgV(H) gene monoclonality may persist. We studied the long-term histological and molecular follow-up of 24 patients and the possible association of t(11;18) with the persistent monoclonality. PATIENTS AND METHODS From January 1994, 24 untreated patients with stage I low-grade gastric MALT lymphoma associated with H. pylori were prospectively studied. They all received eradication treatment and were sequentially followed-up with endoscopies for histological and molecular studies. Rearrangement of the IgV(H) gene was studied by PCR analysis. MALT1 locus alterations were studied by FISH. RESULTS Twenty-two of the 24 patients (91%) achieved disappearance of the lymphoma. Eighteen (82%) of the 22 histologically cured patients and 16 of the 19 (84%) with long follow-up had monoclonality. Three patterns of development of IgV(H) gene rearrangements were observed: four patients (21%) had polyclonal rearrangements; eight (58%) had maintained/intermittent monoclonality and four (21%) had occasional monoclonality, mostly after H. pylori reinfection. Only one patient (6%) with persistent monoclonality relapsed. The remaining 18 patients maintained the remission, despite the persistent monoclonality in 15, for a median of 66 months (range 20-113). t(11;18) was not found in any of the patients with persistent monoclonality. Time and the number of endoscopies performed were not related with the occurrence of monoclonality. CONCLUSIONS In stage I low-grade gastric MALT lymphoma eradication of H. pylori achieves prolonged histological remission in 90% of patients, but molecular remission is not accomplished in most cases. Molecular disease persists for years, but is not associated with t(11;18).
The Impacts of Rapid Population Growth on Poverty, Food Production, and the Environment

This survey paper updates what is known about the linkages between demographic change (changes in age distributions, the size and the growth rate of population, and migration) and the capacity of countries to provide food, to reduce poverty, and to maintain their environment. This assessment applies a longer-run perspective, and considers political a well as economic changes and impacts.