Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis and Validation of Critical Signatures and Immune Cell Infiltration Characteristics in Doxorubicin-Induced Cardiotoxicity by Integrating Bioinformatics and Machine Learning

Purpose Doxorubicin-induced cardiotoxicity (DIC) is a severe side reaction in cancer chemotherapy that greatly impacts the well-being of cancer patients. Currently, there is still an insufficiency of effective and reliable biomarkers in the field of clinical practice for the early detection of DIC. This study aimed to determine and validate the potential diagnostic and predictive values of critical signatures in DIC. Methods We obtained high-throughput sequencing data from the GEO database and performed data analysis and visualization using R software, GO, KEGG and Cytoscape. Machine learning methods and weighted gene coexpression network (WGCNA) were used to identify key genes for diagnostic model construction. Receiver operating characteristic (ROC) analysis and a nomogram were used to assess their diagnostic values. A multiregulatory network was built to reveal the possible regulatory relationships of critical signatures. Cell-type identification by estimating relative subsets of RNA transcript (CIBERSORT) analysis was used to investigate differential immune cell infiltration. Additionally, a cell and animal model were constructed to investigate the relationship between the identified genes and DIC. Results Among the 3713 differentially expressed genes, three key genes (CSGALNACT1, ZNF296 and FANCB) were identified. A nomogram and ROC curves based on three key genes showed excellent diagnostic predictive performance. The regulatory network analysis showed that the TFs CREB1, EP300, FLI1, FOXA1, MAX, and MAZ modulated three key genes. An analysis of immune cell infiltration indicated that many immune cells (activated NK cells, M0 macrophages, activated dendritic cells and neutrophils) might be related to the progression of DIC. Furthermore, there may be various degrees of correlation between the three critical signatures and immune cells. RT‒qPCR demonstrated that the mRNA expression of CSGALNACT1 and ZNF296 was significantly upregulated, while FANCB was significantly downregulated in DOX-treated cardiomyocytes in vitro and in vivo. Conclusion Our study suggested that the differential expression of CSGALNACT1, ZNF296 and FANCB is associated with cardiotoxicity and is also involved in immune cell infiltration in DIC. They might be potential biomarkers for the early occurrence of DIC.
A comprehensive meta-analysis of transcriptome data to identify signature genes associated with pancreatic ductal adenocarcinoma

Purpose Pancreatic ductal adenocarcinoma (PDAC) has a five-year survival rate of less than 5%. Absence of symptoms at primary tumor stages, as well as high aggressiveness of the tumor can lead to high mortality in cancer patients. Most patients are recognized at the advanced or metastatic stage without surgical symptom, because of the lack of reliable early diagnostic biomarkers. The objective of this work was to identify potential cancer biomarkers by integrating transcriptome data. Methods Several transcriptomic datasets comprising of 11 microarrays were retrieved from the GEO database. After pre-processing, a meta-analysis was applied to identify differentially expressed genes (DEGs) between tumor and nontumor samples for datasets. Next, co-expression analysis, functional enrichment and survival analyses were used to determine the functional properties of DEGs and identify potential prognostic biomarkers. In addition, some regulatory factors involved in PDAC including transcription factors (TFs), protein kinases (PKs), and miRNAs were identified. Results After applying meta-analysis, 1074 DEGs including 539 down- and 535 up-regulated genes were identified. Pathway enrichment analyzes using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that DEGs were significantly enriched in the HIF-1 signaling pathway and focal adhesion. The results also showed that some of the DEGs were assigned to TFs that belonged to 23 conserved families. Sixty-four PKs were identified among the DEGs that showed the CAMK family was the most abundant group. Moreover, investigation of corresponding upstream regions of DEGs identified 11 conserved sequence motifs. Furthermore, weighted gene co-expression network analysis (WGCNA) identified 8 modules, more of them were significantly enriched in Ras signaling, p53 signaling, MAPK signaling pathways. In addition, several hubs in modules were identified, including EMP1, EVL, ELP5, DEF8, MTERF4, GLUP1, CAPN1, IGF1R, HSD17B14, TOM1L2 and RAB11FIP3. According to survival analysis, it was identified that the expression levels of two genes, EMP1 and RAB11FIP3 are related to prognosis. Conclusion We identified several genes critical for PDAC based on meta-analysis and system biology approach. These genes may serve as potential targets for the treatment and prognosis of PDAC.
Paradox mindset as an equalizer: A moderated mediated perspective on workplace ostracism

Incorporating the conservation of resources theory as a comprehensive framework, this study investigates a cohesive conceptual model analyzing the impact of workplace ostracism on employees’ innovative work behavior (IWB). The investigation further delves into the mediating influence of perceived control and the moderating roles of paradox mindset and support for innovation. Data collection employed a survey approach involving three-time lags through questionnaires administered to 513 employees within Pakistan’s public sector organizations. The hypothesized relationships were evaluated using conditional process modeling. Our research sheds light on how perceived control mitigates the negative impact of ostracism. The paradox mindset is identified as a key moderator influencing cognitive resources and navigating ostracism. Support for innovation enhances the link between perceived control and innovative work behavior. In addition, limitations, future research directions, and implications of our findings for fostering creative workplaces are also discussed.
Dimerization of a 5-kDa domain defines the architecture of the 5-MDa gammaproteobacterial pyruvate dehydrogenase complex

The Escherichia coli pyruvate dehydrogenase complex (PDHc) is a ~5 MDa assembly of the catalytic subunits pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3). The PDHc core is a cubic complex of eight E2 homotrimers. Homodimers of the peripheral subunits E1 and E3 associate with the core by binding to the peripheral subunit binding domain (PSBD) of E2. Previous reports indicated that 12 E1 dimers and 6 E3 dimers bind to the 24-meric E2 core. Using an assembly arrested E2 homotrimer (E23), we show that two of the three PSBDs in the E23 dimerize, that each PSBD dimer cooperatively binds two E1 dimers, and that E3 dimers only bind to the unpaired PSBD in E23. This mechanism is preserved in wild-type PDHc, with an E1 dimer:E2 monomer:E3 dimer stoichiometry of 16:24:8. The conserved PSBD dimer interface indicates that PSBD dimerization is the previously unrecognized architectural determinant of gammaproteobacterial PDHc megacomplexes.
Positive and negative emotions during the COVID-19 pandemic: A longitudinal survey study of the UK population

The COVID-19 pandemic has had a profound impact on society; it changed the way we work, learn, socialise, and move throughout the world. In the United Kingdom, policies such as business closures, travel restrictions, and social distance mandates were implemented to slow the spread of COVID-19 and implemented and relaxed intermittently throughout the response period. While negative emotions and feelings such as distress and anxiety during this time of crisis were to be expected, we also see the signs of human resilience, including positive feelings like determination, pride, and strength. A longitudinal study using online survey tools was conducted to assess people’s changing moods during the pandemic in the UK. The Positive and Negative Affect Schedule (PANAS) was used to measure self-reported feelings and emotions throughout six periods (phases) of the study from March 2020 to July 2021. A total of 4,222 respondents participated in the survey, while a sub-group participated in each of the six study phases (n = 167). The results were analysed using a cross-sectional study design for the full group across each study phase, while prospective cohort analysis was used to assess the subset of participants who voluntarily answered the survey in each of the six study phases (n = 167). Gender, age and employment status were found to be most significant to PANAS score, with older people, retirees, and women generally reporting more positive moods, while young people and unemployed people generally reported lower positive scores and higher negative scores, indicating more negative emotions. Additionally, it was found that people generally reported higher positive feelings in the summer of 2021, which may be related to the relaxation of COVID-19-related policies in the UK as well as the introduction of vaccines for the general population. This study is an important investigation into what allows for positivity during a crisis and gives insights into periods or groups that may be vulnerable to increased negative states of emotions and feelings.
Long-Term Rainfall Variability in the Eastern Gangetic Plain in Relation to Global Temperature Change

ABSTRACT India’s annual weather cycle consists mainly of wet and dry periods with monsoonal rains being one of the significant wet periods that shows strong spatiotemporal variability. This study includes the climatological characteristics, fluctuation features, and periodic cycles of annual, seasonal, and monthly rainfall of seven river basins across the eastern Gangetic Plain (EGP) using the longest possible instrumental area-averaged monthly rainfall series (1829–2012). Understanding the relationships between these parameters and global tropospheric temperature changes and El Niño and La Niña climatic signals is also attempted. Climatologically, mean annual rainfall in the EGP varies from 1070.5 mm in the Tons River basin to 1508.6 mm in the Subarnarekha River basin. The highest rainfall in the EGP occurs during monsoon (1188 mm). The annual rainfall in all river basins and monsoon rainfall in four river basins is normally distributed. Annual and monsoonal rainfall in the Brahmani and Son River basins show a significant decreasing long-term trend. Over the last 20 years, annual rainfall in all river basins and monsoonal rainfall in five river basins show a decreasing trend. The power spectra for all rainfall series are characterized by consistent significant wavelength peaks at 3–5 years, 10–20 years, 40 years, and more than 80 years. Short-term fluctuations with a period less than 10 years is the major contributor to total variance in annual and/or monsoon rainfall (77.6%), followed by decadal variations with a period of 10–30 years (13.1%) and a long-term trend with a period greater than 30 years (9.3%). Temperature and thickness gradients from the Tibet–Himalaya–Karakoram–Hindu Kush highlands to eight strong highs show a significant correlation with rainfall during the onset and withdrawal phases of summer monsoon in the EGP.
Psychobehavioral factors and family functioning in mucopolysaccharidosis: preliminary studies

Introduction Mucopolysaccharidoses (MPS) constitute a group of progressive and multisystemic inherited metabolic diseases that profoundly affect both the mental health of patients and the wellbeing of their families. This study aims to evaluate the impact of MPS on family functioning and related factors. Methods and results Twenty-five patients with MPS, including types I (n = 4), II (n = 11), IIIB (n = 2), IVA (n = 3), and VI (n = 5), and their families participated in this study. The mean patient age was 13 years [standard deviation (SD): 7.7 years]. Behavioral and emotional problems were noted in 9.1% of all patients. While the type of MPS did not directly influence mental problems, the presence of neuronal involvement did (p = 0.006). Patients with MPS III exhibited difficulties primarily in emotional areas, conduct, hyperactivity, and peer problems. Importantly, both patients with MPS II and those with MPS III experienced a significant impact on communication [mean scores for communication domain: MPS II, 35.6 (SD: 24.3); MPS III, 35.0 (SD: 22.6)]; poorer communication was directly linked to worse adaptive behavior (p = 0.012), and worse adaptive behavior was associated with lower quality of life (p = 0.001). Quality of life and caregiver burden among family members did not significantly differ across MPS types; however, higher caregiver burden was negatively associated with quality of life (p = 0.002). Concerning family functioning, the most impacted domains included independence, intellectual/cultural orientation, activity/recreation, and expressiveness. Domain scores did not vary based on MPS type, treatment, or neurological involvement. Quality-of-life scores were positively associated with the cultural/intellectual domain score. Conclusion The impacts of quality of life and family extend beyond clinical characteristics and MPS type, strongly influenced by patient cognition and communication, as well as type of family functioning, especially those with greater cultural/intellectual skills of their family members. A multidisciplinary approach addressing the broader needs of individuals with MPS becomes essential. Techniques aimed at improving communication, including prompt interventions such as speech therapy and augmentative and alternative communication strategies, can contribute to overall family functioning improvement.
AMPK phosphorylation of FNIP1 (S220) controls mitochondrial function and muscle fuel utilization during exercise

Exercise-induced activation of adenosine monophosphate–activated protein kinase (AMPK) and substrate phosphorylation modulate the metabolic capacity of mitochondria in skeletal muscle. However, the key effector(s) of AMPK and the regulatory mechanisms remain unclear. Here, we showed that AMPK phosphorylation of the folliculin interacting protein 1 (FNIP1) serine-220 (S220) controls mitochondrial function and muscle fuel utilization during exercise. Loss of FNIP1 in skeletal muscle resulted in increased mitochondrial content and augmented metabolic capacity, leading to enhanced exercise endurance in mice. Using skeletal muscle–specific nonphosphorylatable FNIP1 (S220A) and phosphomimic (S220D) transgenic mouse models as well as biochemical analysis in primary skeletal muscle cells, we demonstrated that exercise-induced FNIP1 (S220) phosphorylation by AMPK in muscle regulates mitochondrial electron transfer chain complex assembly, fuel utilization, and exercise performance without affecting mechanistic target of rapamycin complex 1–transcription factor EB signaling. Therefore, FNIP1 is a multifunctional AMPK effector for mitochondrial adaptation to exercise, implicating a mechanism for exercise tolerance in health and disease.
Variations in root architecture traits and their association with organ mass fraction of common annual ephemeral species in the desert of northern Xinjiang

Abstract The variation of plant traits is closely related to the trade‐offs between resource acquisition and conservation, as well as the accumulation of biomass. However, there has been a lack of comprehensive insights into the variation patterns, phylogenetic conservatism, and covariation with biomass allocation of root system architecture in desert areas. We examined the root systems of 47 annual ephemeral species and evaluated their biomass allocation and six key root system architecture traits. Our results indicated that the variation in root traits mainly originated from interspecific variation (48.78%–99.76%), but intraspecific variation should not be ignored as to why the contribution rate of root tissue density (RTD) reached 51.22%. The six root traits were mainly loaded on the first and second axes of the principal component analysis (PCA), these traits mainly vary along two dimensions. The highest interspecific variation is in RTD (51.63%) and the lowest in topological index (TI; 5.92%). The intraspecific variation value and range of specific root length (SRL), specific root area (SRA), and RTD were significantly higher than TI (p < .05), and they are not limited by phylogenetic relationships (0< K < 1, p > .05). The SRA is positively correlated with SRL (r = .72, p < .001) and negatively correlated with RTD (r = −.57, p < .05). The LMF is positively correlated with SRL, and SRA demonstrated the coordination between water consumption and acquisition. The positive correlation between RMF and MRD indicated the coordination of root carbon investment with exploring soil vertical space. The multi‐dimensional variation of root traits, divergence of RTDs, and convergence of TI are important ecological strategies for annual short‐lived plants to adapt to heterogeneous desert habitats. Meanwhile, these plants achieve optimal access to scarce resources through the high plasticity of resource acquisition (e.g., SRL and SRA) and conservation traits (e.g., RTD), as well as the trade‐offs between them and organ mass fraction.
PENGARUH MOTIVASI, KUALITAS LAYANAN, DAN KEPUASAN WISATAWAN TERHADAP IMAGE DAERAH TUJUAN WISATA

Pertumbuhan industri pariwisata yang semakin pesat dapat mendorong pembangunan daerah, meningkatkan devisa negara, meningkatkan kesejahteraan dan kemakmuran rakyat serta dapat menambah rasa cinta tanah air, bangsa dan nilai-nilai budaya. Oleh karena itu, pengelolaan dan penguatan image suatu Daerah Tujuan Wisata (DTW) merupakan langkah yang harus mendapatkan perhatian yang serius secara terus menerus. Tujuan dari penelitian ini adalah menguji dan menganalisis pengaruh nilai motivasi wisatawan, kualitas layanan dan kepuasan wisatawan secara langsung dan tidak langsung terhadap image DTW. Data yang dibutuhkan untuk penelitian ini diperoleh dari sampel sebanyak 200 responden. Adapun populasi terdiri dari seluruh wisatawan yang berasal dari luar Malang Raya dan bertujuan untuk berwisata ke DTW Malang Raya. Data dianalisis menggunakan metode statistik deskriptif dan inferensial. Temuan penelitian ini menjelaskan bahwa semakin meningkat nilai motivasi wisatawan dan kualitas layanan wisata akan dapat meningkatkan nilai kepuasan wisatawan terhadap Daerah Tujuan Wisata. Di samping itu meningkatnya kualitas layanan wisata dapat secara langsung meningkatkan image wisatawan terhadap DTW. Selanjutnya melalui kepuasan wisatawan, peningkatan nilai motivasi wisatawan dan kualitas layanan secara tidak langsung dapat meningkatkan image wisatawan terhadap DTW.
Arbuscular mycorrhizae in mine revegetation as affected by fungal source plant species and abiotic stress

ii Acknowledgements. iii List of Figures. vi List of Tables. vii CHAPTER 1: Introduction: Arbuscular Mycorrhizae in Hardrock Mine Waste Revegetation.1 Arbuscular Mycorrhizae and Plant Communities. 2 Positive Interactions in Stressful Conditions. 3 Revegetation of Hardrock Mine Wastes. 4 Scope of Dissertation. 6 References.10 CHAPTER 2: Plant Growth Effects Due to Arbuscular Mycorrhizal and Microbial Inoculum From Metals-Contaminated and Uncontaminated Sources.14 Abstract.15 Introduction.16 Methods.20 Results. 25 Discussion.27 Acknowledgements. 31 References.32 CHAPTER 3: Mycorrhizal Dependency of Deschampsia cespitosa Across a pHand Metals-induced Stress Gradient. 41 Abstract.42 Introduction.43 Methods.47 Results.52 Discussion.55 Acknowledgements.61 References.62 CHAPTER 4: Mycorrhizal Source Effects on Six Native Plants with Potential for Mine Tailings Revegetation.71 Abstract.72 Introduction.73 iv Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Methods.77 Results.81 Discussion.83 Acknowledgements. 87 References.88 CHAPTER 5: Effects of Compost and Arbuscular Mycorrhizal Inocula on Simple Native Plant Communities Transplanted for Mine Waste Revegetation.97 Abstract.98 Introduction.99 Methods.104 Results.111 Discussion.116 Acknowledgements. 120 References.121 CHAPTER 6: Incorporating Ecological Concepts into Mine Restoration Practices.132 Introduction.133 Ecological Restoration of Mine Wastes.134 Information Exchange: Basic Science and Management. 135 The internship.137 Workshop Assessment. 141 Conclusions and Recommendations.145 References.148 APPENDIX A: Quality Assurance / Quality Control for ICP-AES of Soil Water Extracts and Soil and Plant Digest.149 APPENDIX B: Workshop Evaluation Form.153
Hồ Chí Minh City

Hồ Chi Minh City Amateur Astronomy Club, Vietnam Traduction-Translation-Dịch bản vietnamien (definitions) francais > vietnamien vietnamien > francais english > vietnamien vietnamien > english Hồ Chi Minh City longitude : 106,7 E Latitude : 10° 48′ = 10,8°N date : 16/03/2008 angle : 12,6° Lafrancaise latitude : 44°08' = 44,133° date : 16/03/2006 gnomon : 100cm ombre : 103cm angle : 45°51' = 45,85° difference of latitude : 44,133 -10,8 = 33,333° distance : (.)
The association between consideration of future consequences and food intake is mediated by food choice motives in a French adult population

Objectives: Consideration of future consequences (CFC) distinguishes individuals who adopt behaviours based on immediate needs and concerns from individuals who consider the future consequences of their behaviours. We aimed to assess the association between CFC and diet, and testing the mediating role of food choice motives on this relationship. Design: Individuals (aged ≥ 18 years) completed the CFC-12 questionnaire in 2014, at least three 24-h dietary records, and a food choice motive questionnaire. A multiple mediator analysis allowed to assess the mediating effect of food choice motives on the cross-sectional association between CFC and diet, adjusted for socio-demographic factors. Setting: Data from the NutriNet-Santé cohort study. Participants: 27 330 participants. Results: CFC was associated with all food choice motives (P < 0·001), with the strongest positive associations for avoidance for environmental reasons, absence of contaminants and health motives and the strongest negative associations for innovation and convenience. Positive total effects were found between CFC and the consumption of healthy food groups (fruits and vegetables, whole-grain foods, legumes), and negative total effects for alcohol, meat and poultry and processed meat (P < 0·001). CFC was positively associated with diet quality (P < 0·001). Across food groups, major mediators of these relationships were higher health (8·4–32·6%), higher environmental (13·7–22·1 %) and lower innovation (7·3–25·1 %) concerns. Conclusions: CFC was associated with healthier dietary intake, essentially mediated by a greater motivation of future-oriented participants for self-centred and altruistic outcomes, including health and environment. Focusing on the awareness of future benefits in public health interventions might lead to healthier dietary behaviours.