Miracle-dz/UniFAR-FTUs huggingface dataset:
Table top system for angle resolved light scattering measurement

﻿Die Messung und Analyse von Streulicht hat in den vergangenen Jahren wesentlich an Einfluss gewonnen. Der Mangel an Messsystemen die sowohl flexibel als auch kompakt sind, motivierte diese Arbeit zur Entwicklung eines winkelauflosenden Table-Top Streulichtmesssystems mit dazugehorigen Auswertemethodiken.Das realisierte Table-Top Goniometer weist kompakte Abmase von <0,8x0,8x0,8 m³ auf und ermoglicht die Messung von 3D-Streulichtverteilungen bei Beleuchtungwellenlangen von 405 nm, 532 nm und 640 nm. Mit erreichten Sensitivitaten, die nur durch die Luftstreuung begrenzt sind, wurde eine Dynamik von 14 Grosenordnungen erzielt. Die kompakte Konstruktion wurde durch ein spezielles Achsdesign und uber Faltung der Strahlengange erreicht. Messunsicherheit und Instrumentesignatur wurden anhand von neu entwickelten Modellen und Simulationen analysiert. Darauf aufbauend erfolgten Optimierungen.Basierend auf dem Frequenzmultiplex-Prinzip wurde ein Konzept zur Ermoglichung paralleler Streulichtmessungen mit hoher Sensitivitat und Dynamik entwickelt. Hiermit lasst sich das gestreute Licht unterschiedlicher Beleuchtungswellenlangen und/oder -polarisationen in verschiedenen Messkanalen gleichzeitig detektieren.Es wurde eine streulichtbasierte Analysemethode anisotroper Oberflachen eingefuhrt, die einen direkten Bezug zu optischen und rauheitsbeschreibenden Eigenschaften aufweist. Hiermit liesen sich erstmals Rauheitskomponenten unterschiedlicher Ursache bei ultraprazisionsbearbeiteten diamantgedrehten Oberflachen als Funktion der Ortsfrequenz trennen. Der Einfluss von Anisotropie bei unterschiedlichen Beleuchtungswellenlangen wurde untersucht.Uber Streulichtanalyse mit mehreren Beleuchtungswellenlangen wurde eine hochrobuste Rauheitscharakterisierung demonstriert. Hiermit liesen sich Kontaminationen auf beschichteten und unbeschichteten Substraten sowie Rauheitswachstum in Dunnschichtfilmen nachweisen.Es wurde gezeigt, dass sich Diamantschliffe uber die Auswertung von 3D-Streulichtverteilungen charakterisieren lassen. Es konnen beispielsweise die Politurgute einzelner Facetten sowie die optische Performance von Brillanten bewertet und klassifiziert werden.
Nanostructures on Fluoropolymer Nanotextile Prepared Using a High-Energy Excimer Laser

This study is focused on polytetrafluoroethylene (PTFE) porous nanotextile and its modification with thin, silver sputtered nanolayers, combined with a subsequent modification with an excimer laser. The KrF excimer laser was set to single-shot pulse mode. Subsequently, the physico chemical properties, morphology, surface chemistry, and wettability were determined. Minor effects of the excimer laser on the pristine PTFE substrate were described, but significant changes were observed after the application of the excimer laser to the polytetrafluoroethylene with sputtered silver, where the formation of a silver nanoparticles/PTFE/Ag composite was described, with a wettability similar to that of a superhydrophobic surface. Both scanning electron microscopy and atomic force microscopy revealed the formation of superposed globular structures on the polytetrafluoroethylene lamellar primary structure, which was also confirmed using energy dispersive spectroscopy. The combined changes in the surface morphology, chemistry, and thus wettability induced a significant change in the PTFE’s antibacterial properties. Samples coated with silver and further treated with the excimer laser 150 mJ/cm2 inhibited 100% of the bacterial strain E. coli. The motivation of this study was to find a material with flexible and elastic properties and a hydrophobic character, with antibacterial properties that could be enhanced with silver nanoparticles, but hydrophobic properties that would be maintained. These properties can be used in different types of applications, mainly in tissue engineering and the medicinal industry, where water-repellent materials may play important roles. This synergy was achieved via the technique we proposed, and even when the Ag nanostructures were prepared, the high hydrophobicity of the system Ag-polytetrafluorethylene was maintained.
Incidental Axillary Dose of Tomotherapy in Hypofractionated Whole Breast Radiotherapy for Early Breast Cancer: A Dosimetrical Analysis

Background and Objectives: Intensity-modulated radiation therapy (IMRT) is becoming a more common method of performing whole breast irradiation (WBI) for early breast cancer. This study aimed to examine the incidental dose to the axillary region using tomotherapy, a unique form of IMRT. Patients and Methods: This study included 30 patients with early-stage breast cancer who underwent adjuvant WBI using TomoDirect IMRT. A hypofractionation scheme of 42.4 Gy delivered in 16 fractions was prescribed. The plan comprised of two parallel-opposed beams, along with two additional beams positioned anteriorly at gantry angles of 20° and 40° from the medial beam. The incidental dose received at axillary levels I, II, and III was evaluated using several dose-volume parameters. Results: The study participants had a median age of 51 years, and 60% had left-sided breast cancer. The mean dose of the axilla for levels I, II, and III were 15.5 ± 4.8 Gy, 14.9 ± 4.2 Gy, and 1.5 ± 1.6 Gy, respectively. Adequate coverage of the axilla, defined as V95%[%], was achieved for 4.7 ± 3.9%, 4.8 ± 3.7%, and 0 ± 0% for levels I, II, and III, respectively. The results were compared with those of previously published studies, and the axillary mean dose and V95%[%] of TomoDirect IMRT were low, comparable to other IMRT techniques, and lower than those of traditional tangential therapy. Conclusions: While incidental axillary radiation during WBI has been proposed to assist in regional disease control, the TomoDirect plan was demonstrated to decrease this dose, and a hypofractionation scheme would further lower its biological effectiveness. Future clinical studies should incorporate dosimetrical analysis of incidental axillary dose, in order to facilitate hypofractionated IMRT planning with risk-adjusted axilla coverage in early breast cancer.
Near-infrared scattering effects of mesoporous TiO2 analyzed by optical coherence tomography

Optical properties of mesoporous TiO2 scatters with average diameters of 20 nm, 150 nm, 300 nm, and 500 nm are measured by optical coherence tomography system at the wavelength of 853 nm. The scattering coefficient can be estimated by utilizing the extend Huygens Fresnel theory with consideration of single and multiple scattering effects, and the g factors are calculated based on the Mie scattering model. After fitting algorithm, by increasing the sizes of mesoporous TiO2 scatters from 20, 150, 300, to 500 nm, the scattering coefficient could also be extracted as from 13.5±0.6, 16.5±0.6, to 20.2±0.6 mm-1, and then decreased to 18.5±0.6 mm-1, which are accompanied with the enhancement of multiple-scattering contribution from 5%, 10%, to 40%, and then decreased to 20%, respectively.
Editing of rice (Oryza sativa L.) OsMKK3 gene using CRISPR/Cas9 decreases grain length by modulating the expression of photosystem components

Grain size is one of the most important agronomic traits for grain yield determination in rice. To better understand the proteins that are regulated by the grain size regulatory gene OsMKK3, this gene was knocked out using the CRISPR/Cas9 system, and tandem mass tag (TMT) labeling combined with liquid chromatograph‐tandem mass spectrometry analysis was performed to study the regulation of proteins in the panicle. Quantitative proteomic screening revealed a total of 106 differentially expressed proteins (DEPs) via comparison of the OsMKK3 mutant line to the wild‐type YexiangB, including 15 and 91 up‐regulated and down‐regulated DEPs, respectively. Pathway analysis revealed that DEPs were enriched in metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, and photosynthesis. Strong interactions were detected among seven down‐regulated proteins related to photosystem components in the protein‐protein interaction network, and photosynthetic rate was decreased in mutant plants. The results of the liquid chromatography‐parallel reaction monitoring/mass spectromery analysis and western blot analysis were consistent with the results of the proteomic analysis, and the results of the quantitative reverse transcription polymerase chain reaction analysis revealed that the expression levels of most candidate genes were consistent with protein levels. Overall, OsMKK3 controls grain size by regulating the protein content in cells. Our findings provide new candidate genes that will aid the study of grain size regulatory mechanisms associated with the mitogen‐activated protein kinase (MAPK) signaling pathway.
Influence of Surface-Modification via PEGylation or Chitosanization of Lipidic Nanocarriers on In Vivo Pharmacokinetic/Pharmacodynamic Profiles of Apixaban

Nanostructured lipid carriers (NLCs) have been proven to significantly improve the bioavailability and efficacy of many drugs; however, they still have many limitations. These limitations could hinder their potential for enhancing the bioavailability of poorly water-soluble drugs and, therefore, require further amendments. From this perspective, we have investigated how the chitosanization and PEGylation of NLCs affected their ability to function as a delivery system for apixaban (APX). These surface modifications could enhance the ability of NLCs to improve the bioavailability and pharmacodynamic activity of the loaded drug. In vitro and in vivo studies were carried out to examine APX-loaded NLCs, chitosan-modified NLCs, and PEGylated NLCs. The three nanoarchitectures displayed a Higuchi-diffusion release pattern in vitro, in addition to having their vesicular outline proven via electron microscopy. PEGylated and chitosanized NLCs retained good stability over 3 months, versus the nonPEGylated and nonchitosanized NLCs. Interestingly, APX-loaded chitosan-modified NLCs displayed better stability than the APX-loaded PEGylated NLCs, in terms of mean vesicle size after 90 days. On the other hand, the absorption profile of APX (AUC0-inf) in rats pretreated with APX-loaded PEGylated NLCs (108.59 µg·mL−1·h−1) was significantly higher than the AUC0-inf of APX in rats pretreated with APX-loaded chitosan-modified NLCs (93.397 µg·mL−1·h−1), and both were also significantly higher than AUC0-inf of APX-Loaded NLCs (55.435 µg·mL−1·h−1). Chitosan-coated NLCs enhanced APX anticoagulant activity with increased prothrombin time and activated partial thromboplastin time by 1.6- and 1.55-folds, respectively, compared to unmodified NLCs, and by 1.23- and 1.37-folds, respectively, compared to PEGylated NLCs. The PEGylation and chitosanization of NLCs enhanced the bioavailability and anticoagulant activity of APX over the nonmodified NLCs; this highlighted the importance of both approaches.
Local motion signals silence the perceptual solution of global apparent motion

Stimuli for apparent motion can have ambiguity in frame-to-frame correspondences among visual elements. This occurs when visual inputs cause a correspondence problem that allows multiple alternatives of perceptual solutions. Herein we examined the influence of local visual motions on a perceptual solution under such a multistable situation. We repeatedly alternated two frames of stimuli in a circular configuration in which discrete elements in two different colors alternated in space and switched their colors frame by frame. These stimuli were compatible with three perceptual solutions: globally consistent clockwise and counterclockwise rotations and color flickers at the same locations without such global apparent motion. We added a sinusoidal grating continuously drifting within each element to examine whether the perceptual solution for the global apparent motion was affected by the local continuous motions. We found that the local motions suppressed global apparent motion and promoted another perceptual solution that the local elements were only flickering between the two colors and drifting within static windows. It was concluded that local continuous motions as counterevidence against global apparent motion contributed to individuating visual objects and integrating visual features for maintaining object identity at the same location
Enhancing Wound Healing: A Novel Topical Emulsion Combining CW49 Peptide and Lavender Essential Oil for Accelerated Regeneration and Antibacterial Protection

Wound healing is a complex process involving blood cells, extracellular matrix, and parenchymal cells. Research on biomimetics in amphibian skin has identified the CW49 peptide from Odorrana grahami, which has been demonstrated to promote wound regeneration. Additionally, lavender essential oil exhibits anti-inflammatory and antibacterial activities. Given these considerations, we propose an innovative emulsion that combines the CW49 peptide with lavender oil. This novel formulation could serve as a potent topical treatment, potentially fostering the regeneration of damaged tissues and providing robust antibacterial protection for skin wounds. This study investigates the physicochemical properties, biocompatibility, and in vitro regenerative capacity of the active components and the emulsion. The results show that the emulsion possesses appropriate rheological characteristics for topical application. Both the CW49 peptide and lavender oil exhibit high viability in human keratinocytes, indicating their biocompatibility. The emulsion induces hemolysis and platelet aggregation, an expected behavior for such topical treatments. Furthermore, the lavender-oil emulsion demonstrates antibacterial activity against both Gram-positive and Gram-negative bacterial strains. Finally, the regenerative potential of the emulsion and its active components is confirmed in a 2D wound model using human keratinocytes. In conclusion, the formulated emulsion, which combines the CW49 peptide and lavender oil, shows great promise as a topical treatment for wound healing. Further research is needed to validate these findings in more advanced in vitro models and in vivo settings, potentially leading to improved wound-care management and novel therapeutic options for patients with skin injuries.
Astaxanthin production from the microalga Haematococcus lacustris with a dual substrate mixotrophy strategy

This study investigates the development of dual‐substrate mixotrophy strategy to cultivate the microalga Haematococcus lacustris for astaxanthin production. The influence of different concentrations of acetate and pyruvate on biomass productivity was first assessed individually, and then both substrates were used together to improve biomass growth in the green phase and astaxanthin accumulation in red the phase. The results showed that dual‐substrates mixotrophy significantly increased the biomass productivity during green growth phase up to 2‐fold compared to phototrophic controls. Furthermore, supplementation of dual‐substrate to the red phase increased astaxanthin accumulation by 10% in the dual‐substrate group compared to single‐substrate acetate and no substrate. This dual‐substrate mixotrophy approach shows promise for cultivating Haematococcus for commercial production of biological astaxanthin in indoor closed systems.
Prevalence and Etiological Characteristics of Norovirus Infection in China: A Systematic Review and Meta-Analysis

Norovirus is a common cause of sporadic cases and outbreaks of gastroenteritis worldwide, although its prevalence and the dominant genotypes responsible for gastroenteritis outbreaks remain obscure. A systematic review was conducted on norovirus infection in China between January 2009 and March 2021. A meta-analysis and beta-binomial regression model were used to explore the epidemiological and clinical characteristics of norovirus infection and the potential factors contributing to the attack rate of the norovirus outbreaks, respectively. A total of 1132 articles with 155,865 confirmed cases were included, with a pooled positive test rate of 11.54% among 991,786 patients with acute diarrhea and a pooled attack rate of 6.73% in 500 norovirus outbreaks. GII.4 was the predominant genotype in both the etiological surveillance and outbreaks, followed by GII.3 in the etiological surveillance, and GII.17 in the outbreaks, with the proportion of recombinant genotypes increasing in recent years. A higher attack rate in the norovirus outbreaks was associated with age group (older adults), settings (nurseries, primary schools, etc.) and region (North China). The nation-wide pooled positive rate in the etiological surveillance of norovirus is lower than elsewhere in the global population, while the dominant genotypes are similar in both the etiological surveillance and the outbreak investigations. This study contributes to the understanding of norovirus infection with different genotypes in China. The prevention and control of norovirus outbreaks during the cold season should be intensified, with special attention paid to and enhanced surveillance performed in nurseries, schools and nursing homes from November to March.
Rapid In Vitro Assessment of Antimicrobial Drug Effect Bridging Clinically Relevant Pharmacokinetics: A Comprehensive Methodology

Rapid in vitro assessment of antimicrobial drug efficacy under clinically relevant pharmacokinetic conditions is an essential element of both drug development and clinical use. Here, we present a comprehensive overview of a recently developed novel integrated methodology for rapid assessment of such efficacy, particularly against the emergence of resistant bacterial strains, as jointly researched by the authors in recent years. This methodology enables rapid in vitro assessment of the antimicrobial efficacy of single or multiple drugs in combination, following clinically relevant pharmacokinetics. The proposed methodology entails (a) the automated collection of longitudinal time–kill data in an optical-density instrument; (b) the processing of collected time–kill data with the aid of a mathematical model to determine optimal dosing regimens under clinically relevant pharmacokinetics for single or multiple drugs; and (c) in vitro validation of promising dosing regimens in a hollow fiber system. Proof-of-concept of this methodology through a number of in vitro studies is discussed. Future directions for the refinement of optimal data collection and processing are discussed.
Posterior Spinal Fusion Surgery for Neuromuscular Disease Patients with Severe Scoliosis Whose Cobb Angle Was over 100 Degrees

Background and objectives: Patients with neuromuscular diseases usually have progressive neuromuscular scoliosis (NMS), requiring invasive surgery. Some patients present with severe scoliosis at the time of consultation and are difficult to treat. Posterior spinal fusion (PSF) surgery combined with anterior release and pre- or intraoperative traction would be effective for severe spinal deformities but would be invasive. This study aimed to evaluate the outcomes of PSF-only surgery for patients with severe NMS with a Cobb angle > 100°. Materials and Methods: Thirty NMS patients (13 boys and 17 girls; mean age 13.8 years) who underwent PSF-only surgery for scoliosis with a Cobb angle > 100° were included. We reviewed the lower instrumented vertebra (LIV), duration of surgery, blood loss, perioperative complications, preoperative clinical findings, and radiographic findings, including Cobb angle and pelvic obliquity (PO) in the sitting position pre- and postoperatively. The correction rate and correction loss of the Cobb angle and PO were also calculated. Results: The mean duration of surgery was 338 min, intraoperative blood loss was 1440 mL, preoperative %VC was 34.1%, FEV1.0 (%) was 91.5%, and EF was 66.1%. There were eight cases of perioperative complications. The Cobb angle and PO correction rates were 48.5% and 42.0%, respectively. We divided the patients into two groups: the L5 group, in which the LIV was L5, and the pelvis group, in which the LIV was the pelvis. The duration of surgery and PO correction rate in the pelvis group were significantly higher than those in the L5 group. Conclusions: Patients with severe NMS demonstrated severe preoperative restrictive ventilatory impairments. PSF surgery without anterior release or any intra-/preoperative traction showed satisfactory outcomes, including acceptable scoliosis correction and improved clinical findings, even in patients with extremely severe NMS. Instrumentation and fusion to the pelvis for severe scoliosis in patients with NMS showed good PO correction and low correction loss of Cobb angle and PO, but a longer duration of surgery.
Effect of Monomer Type on the Synthesis and Properties of Poly(Ethylene Furanoate)

This work aimed to produce bio-based poly(ethylene furanoate) (PEF) with a high molecular weight using 2,5-furan dicarboxylic acid (FDCA) or its derivative dimethyl 2,5-furan dicarboxylate (DMFD), targeting food packaging applications. The effect of monomer type, molar ratios, catalyst, polycondensation time, and temperature on synthesized samples’ intrinsic viscosities and color intensity was evaluated. It was found that FDCA is more effective than DMFD in producing PEF with higher molecular weight. A sum of complementary techniques was employed to study the structure–properties relationships of the prepared PEF samples, both in amorphous and semicrystalline states. The amorphous samples exhibited an increase in glass transition temperature of 82–87 °C, and annealed samples displayed a decrease in crystallinity with increasing intrinsic viscosity, as analyzed by differential scanning calorimetry and X-ray diffraction. Dielectric spectroscopy showed moderate local and segmental dynamics and high ionic conductivity for the 2,5-FDCA-based samples. The spherulite size and nuclei density of samples improved with increased melt crystallization and viscosity, respectively. The hydrophilicity and oxygen permeability of the samples were reduced with increased rigidity and molecular weight. The nanoindentation test showed that the hardness and elastic modulus of amorphous and annealed samples is higher at low viscosities due to high intermolecular interactions and degree of crystallinity.
Testing methods for 3D scattered data interpolation

This paper is concerned with the evaluation of methods for 3D scattered data interpolation. In particular, we discuss the computational performances in 3D of methods which give superior results for the two-dimensional case. The testing process was carried out by considering the accuracy, the graphical behaviour of the interpolant and the timing. In addition we have taken into account the computational efficiency and the sensitivity respect to the sample. Moreover, in order to evaluate the graphical behaviour, we present an evolutive visualization of the interpolant.