Miracle-dz/UniFAR-FTUs huggingface dataset:
Five‐lipoxygenase inhibitors can mediate apoptosis in human breast cancer cell lines through complex eicosanoid interactions

Many arachidonic acid metabolites function in growth signaling for epithelial cells, and we previously reported the expression of the major arachidonic acid enzymes in human breast cancer cell lines. To evaluate the role of the 5‐lipoxygenase (5‐LO) pathway on breast cancer growth regulation, we exposed cells to insulinlike growth factor‐1 or transferrin, which increased the levels of the 5‐LO metabolite, 5(S)‐hydrooxyeicosa‐6E,8C,11Z,14Z‐tetraenoic acid (5‐HETE), by radioimmunoassay and high‐performance liquid chromatography. Addition of 5‐HETE to breast cancer cells resulted in growth stimulation, whereas selective biochemical inhibitors of 5‐LO reduced the levels of 5‐HETE and related metabolites. Application of 5‐LO or 5‐LO activating protein‐directed inhibitors, but not a cyclooxygenase inhibitor, reduced growth, increased apoptosis, down‐regulated bcl‐2, up‐regulated bax, and increased G1 arrest. Exposure of breast cancer cells to a 5‐LO inhibitor up‐regulated peroxisome proliferator‐activated receptor (PPAR)α and PPARγ expression, and these same cells were growth inhibited when exposed to relevant PPAR agonists. These results suggest that disruption of the 5‐LO signaling pathway mediates growth arrest and apoptosis in breast cancer cells. Additional experiments suggest that this involves the interplay of several factors, including the loss of growth stimulation by 5‐LO products, the induction of PPARγ, and the potential activation of PPARγ by interactions with shunted endoperoxides.
Valproic acid promotes radiosensitization in meningioma stem-like cells

Although meningioma stem-like cells have been isolated and characterized, their therapeutic targeting remains a challenge. Meningioma sphere cells (MgSCs) with cancer stem cells properties show chemo- and radioresistance in comparison with meningioma adherent cells (MgACs). We tested the effect of valproic acid (VPA), a commonly used anti-epileptic drug, which passes the blood brain barrier, on cultured MgSCs. VPA reduced the viability of MgSCs and MgACs. In MgSCs, treatment with VPA increased radio-sensitivity, expression of p-cdc2, p-H2AX and cleaved caspase-3 and PARP. Anchorage-independent growth (AIG) was reduced by VPA. AIG was further reduced by combined treatment with irradiation. Expression of a stem cell marker, Oct4, was reduced by VPA. Oct4 was further decreased by combined treatment with irradiation. These results suggest that VPA may be a potential treatment for meningioma through targeting meningioma stem-like cells.
Intentions to adopt ecopreneurship: moderating role of collectivism and altruism

There is need to understand the importance of sustainability of economies by the students especially undergoing business education and look for avenues that provide support to sustainability, in which ecopreneurship is one. The current study examines the intentions to adopt ecopreneurship while extending theory of planned behavior model by including the dual moderating role of collectivism and altruism while taking developing country context as field of analysis. Data were collected from students of two universities located in a rural district and student participants of entrepreneurship training workshop. Results show that ecopreneurship is mainly driven by subjective norms and self-efficacy while attitude towards ecopreneurship become significant in presence of collectivism and altruism not otherwise. Similarly, the dual moderating role of collectivism and altruism has been identified by the significance of three-way interactions for attitude, subjective norms and self-efficacy with collectivism and altruism respectively.
A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress

Water is essential for plant growth and development. Water deficiency leads to loss of yield and decreased crop quality. To understand water transport mechanisms in plants, we cloned and characterized a novel tonoplast intrinsic protein (TIP) gene from soybean with the highest similarity to TIP2-type from other plants, and thus designated GmTIP2;3. The protein sequence contains two conserved NPA motifs and six transmembrane domains. The expression analysis indicated that this gene was constitutively expressed in all detected tissues, with higher levels in the root, stem and pod, and the accumulation of GmTIP2;3 transcript showed a significant response to osmotic stresses, including 20% PEG6000 (polyethylene glycol) and 100 μM ABA (abscisic acid) treatments. The promoter-GUS (glucuronidase) activity analysis suggested that GmTIP2;3 was also expressed in the root, stem, and leaf, and preferentially expressed in the stele of root and stem, and the core promoter region was 1000 bp in length, located upstream of the ATG start codon. The GUS tissue and induced expression observations were consistent with the findings in soybean. In addition, subcellular localization showed that GmTIP2;3 was a plasma membrane-localized protein. Yeast heterologous expression revealed that GmTIP2;3 could improve tolerance to osmotic stress in yeast cells. Integrating these results, GmTIP2;3 might play an important role in response to osmotic stress in plants.
FPGA Design of an Intra 16 × 16 Module for H.264/AVC Video Encoder

In this paper, we propose novel hardware architecture for intra 16 × 16 module for the macroblock engine of a new video coding standard H.264. To reduce the cycle of intra prediction 16 × 16, transform/quantization, and inverse quantization/inverse transform of H.264, an advanced method for different operation is proposed. This architecture can process one macroblock in 208 cycles for all cases of macroblock type by processing 4 × 4 Hadamard transform and quantization during 16 × 16 prediction. This module was designed using VHDL Hardware Description Language (HDL) and works with a 160 MHz frequency using ALTERA NIOS-II development board with Stratix II EP2S60F1020C3 FPGA. The system also includes software running on an NIOS-II processor in order to implementing the pre-processing and the post-processing functions. Finally, the execution time of our HW solution is decreased by 26% when compared with the previous work.
Gene Expression in the Lung of p53 Mutant Mice Exposed to Cigarette Smoke

We showed previously that p53 mutations play a role in cigarette smoke-related carcinogenesis not only in humans but also in A/J mice. In fact, (UL53–3 × A/J)F1 mice, carrying a dominant-negative germ-line p53 mutation, responded to exposure to environmental cigarette smoke more efficiently than their wild-type (wt) littermate controls in terms of molecular alterations, cytogenetic damage, and lung tumor yield. To clarify the mechanisms involved, we analyzed by cDNA array the expression of 1,185 cancer-related genes in the lung of the same mice. Neither environmental cigarette smoke nor the p53 status affected the expression of the p53 gene, but the p53 mutation strikingly increased the basal levels of p53 nuclear protein in the lung. Environmental cigarette smoke increased p53 protein levels in wt mice only. The p53 mutation enhanced the expression of positive cell cycle regulators in sham-exposed mice, which suggests a physiologic protective role of p53. In environmental cigarette smoke-exposed mice, the p53 mutation resulted in a lack of induction of proapoptotic genes and in overexpression of genes involved in cell proliferation, signal transduction, angiogenesis, inflammation, and immune response. Mutant mice and wt mice reacted to environmental cigarette smoke in a similar manner regarding genes involved in metabolism of xenobiotics, multidrug resistance, and protein repair. Irrespective of the p53 status, environmental cigarette smoke poorly affected the expression of oncogenes, tumor suppressor genes, and DNA repair genes. Taken together, these findings may explain the increased susceptibility of p53 mutant mice to smoke-related alterations of intermediate biomarkers and lung carcinogenesis.
Target Indices for Clinical Ablation in Atrial Fibrillation: Insights From Contact Force, Electrogram, and Biophysical Parameter Analysis

Background—In animal studies of radiofrequency ablation, lesion sizes plateau as the maximum lesion size is reached for an ablation. Lesion parameters are not available in clinical ablations, but preclinical work suggests that these correlate with impedance drop and electrogram attenuation. Characterization of the relationships between catheter contact force, ablation duration, and these surrogate markers of lesion formation may allow us to define targets for effective ablation. Methods and Results—Fifteen patients undergoing first-time radiofrequency ablation for nonparoxysmal atrial fibrillation were studied. All were in atrial fibrillation at the time of the procedure. Ablations were performed with an irrigated-tip contact force–sensing catheter in temperature-controlled mode (temperature limited to 48°C, power to 30 W). Included were 285 left atrial static ablations, 247 with additional impedance data. The ablation force time integral (FTI) correlated with the attenuation of the electrogram with ablation (Spearman &rgr;, –0.14; P=0.02): the relationship plateauing from 500 g·s, a reduction in the electrogram amplitude of 20%. The FTI also correlated with the impedance drop during ablation (Spearman &rgr;, 0.79; P<0.0005): the relationship was logarithmic, the reduction in the impedance with an increasing FTI also plateauing from 500 g·s, an impedance drop of 7.5%. The ablation duration affected the impedance drop at an FTI if the duration was <10 s. Beyond this time point, the FTI achieved rather than the ablation duration or mean contact force applied determined the impedance drop. Conclusions—During nonparoxysmal atrial fibrillation ablation, an FTI of 500 g·s should be targeted with ablation duration of ≥10 s. Clinical Trials Registration—URL: http://clinicaltrials.gov/. Unique Identifier: NCT01587404.
The Low Arousal Approach: A Practitioner's Guide

In a previous report for CareKnowledge, Damian Milton outlined how ‘imagining otherwise’ about autism can lead to more nuanced and autistic-friendly support strategies being devised and implemented. That report showed how support strategies that have been developed without a good understanding of autistic ways of being can lead to dubious intervention goals and processes. It also suggested that when autistic people have difficulties in communicating their needs, a humble, collaborative, reflective and person-centred approach is required. This new report explores the Low Arousal Approach to managing challenging behaviour, with particular reference as to how it can be implemented in work with autistic people. This report aims to first detail the Low Arousal Approach’s origins, theoretical underpinnings and which features constitute a Low Arousal Approach in professional practice. The report then aims to describe how the Low Arousal Approach can, and should, be applied by practitioners when supporting autistic people who display ‘behaviours of concern’. In addition, the report sets out authentic practice examples of how this approach has been applied, and the results that have been obtained.
To Evaluate the Association between Serum Leptin and Insulin/Insulin Resistance in Polycystic Ovarian Syndrome in a Tertiary Care Centre in North India

Background: Polycystic ovarian syndrome (PCOS) a common endocrinological disorder among women in the reproductive age group, is associated with hyperinsulinemia/Insulin resistance, obesity, menstrual irregularities and anovulation. Aim and Objectives: The present study was conducted to evaluate the association between insulin/insulin resistance and leptin among women with PCOS in a sample population of a tertiary care centre in North India. Materials: Ninety newly diagnosed cases of PCOS as per Rotterdam criteria were enrolled in the study, along with ninety normal women of similar age and BMI. Serum insulin, fasting glucose and serum leptin were estimated, Insulin resistance was calculated using (HOMA-IR) the homeostatic model for assessment-insulin resistance. Results: In our study leptin levels were raised in both cases and in controls. Leptin levels correlated positively with BMI among the cases (r=0.21) and also among the controls (r=0.003). In our study PCOS women had higher mean BMI and leptin levels when compared to controls. Insulin levels correlated positively with BMI among the cases (r=0.21) and was statistically significant (P=.004). Insulin levels were higher in women with BMI in the overweight and obese category. Although most of our cases were of lean weight and BMI in the normal range, high insulin and IR was detected in them showing impaired glucose metabolism. Leptin levels were higher in cases group who also had high IR when compared to healthy controls in our study. Leptin levels correlated positively with IR among cases (r=0.25) and this was statistically significant (P=.013). Conclusion: Our study showed higher serum Leptin, insulin and IR among PCOS cases when compared to the control group.
Phonological recovery in Spanish developmental dyslexics through the tip-of-the-tongue paradigm.

BACKGROUND Developmental dyslexics have difficulties accessing and retrieving the phonological form of words, in the absence of a deficit at the semantic level. The aim of this work was to study, through the Tip-of-the-tongue (TOT) paradigm, the problems of lexical access in Spanish-speaking developmental dyslexics and the relationship with their phonological awareness. METHOD A group of developmental dyslexics (14) and other children without reading difficulties (14), aged 7 to 12, performed a picture naming task of medium and low frequency and a task of phonological awareness. RESULTS The results indicated that dyslexic children generally show a greater number of TOT phenomena than the control group. Despite being able to provide semantic information of the drawing, they had difficulties retrieving partial phonological information. CONCLUSIONS These results indicate that developmental dyslexic children have particular difficulty in accessing the phonological form of words, which may be interesting for the development of intervention programs for these children.
Toward High-Impact Corporate Sustainability Research

It is my pleasure to introduce this Special Issue on “Creating Value for Stakeholders: Mechanisms, Measurements, and Performance Implications,” guest-edited by Timo Busch, Jens Hamprecht and Sandra Waddock. On a personal note, this is a “special” issue because it is the first Special Issue published by Organization & Environment during my tenure as editor. It is a “special” issue, in my view, for several, more substantive, reasons, though. First, it is focused on value creation for stakeholders, therefore aiming to move the discourse beyond the traditional domain of the journal, related to “green” management of organizations. The contributions focus on integrated dimensions of impact, including organizational influence on their natural, social, and economic environment. Second, this issue focuses on a specific theory of the firm, the “stakeholder-based view” (SBV; Freeman, 1984; Freeman et al., 2010), which is proposed as a better answer than other established theories (transaction cost economics [TCE], resource-based view [RBV], knowledge-based view [KBV], etc.) to the foundational question of the management field: why do firms exist? What is their overarching purpose? SBV states it very clearly: Firms exist because they are powerful mechanisms for the creation of value, both of economic and non-economic nature, for a plurality of stakeholders, including shareholders. This is all well understood by now but far from being a consensus view among thought leaders in corporate sustainability research. Many are fully convinced that the standard accounts on why firms exist are correct, disagree on the need to adopt a stakeholder logic, and prefer to focus on research questions that either test or assume the relevance of environmental (or even social) performance, simply because it makes business sense, that is, it positively impacts (long term results) firm economic or financial results. This is a critical divide within our own community of scholars. A divide that remains untackled, quietly brushed under the carpet, as we debate the merits of less contentious, narrowly defined, research quests. The third reason why this is, in my view, a “special” issue, is that it puts front and center not only the question of “for whom” should value be created but also the even more critical “what type of value” firms should create. What does the range of diverse forms of value that firms can target in their objective functions (Lankoski & Smith, 2018) look like? How should they go about not only selecting these dimensions of value but also actually co-creating them by “shaking up” and working with their networks of stakeholders (Sulkowski, Edwards, & Freeman, 2018), as well as using externally verified crowdsourcing processes for organizational change and adaptation to sustainability standards (Rawhouser, Cummings, & Marcus, 2018).
Examining the Impacts of Pokémon Go on the Physical Health and Social Interaction among College Students

Pokémon Go arguably depicts the most popular adoption of augmented reality (AR) technology on any platform and particularly mobile games during the conduct of this research. The mobile location-based game was launched in July 2016, to the delight of millions of people globally. Pokémon Go requires people to physically navigate the real world in the course of playing the game; this was a relatively new experience for many especially as a mobile game. The need to physically navigate the real world in Pokémon Go prompted the quest to examine what impacts Pokémon Go has on college students. Specifically, we focus on the impact on their social interaction and physical activity as there were studies that have shown that traditional mobile games and video games have made people become anti-social, adopt sedentary lifestyles resulting in various issues in social and health aspects. This research aims to acquire a better understanding of how AR games like Pokémon Go impacts social interaction and physical activity, and to recommend guidelines for developing similar augmented reality games to promote social interaction and physical activity. Semi-structured interviews were conducted with twenty participants attending a college in the mid-western region of the United States of America. Our findings revealed that Pokémon Go encouraged increased social interaction among family, friends and strangers. Participants also enjoyed some health and educational benefits as a result of playing Pokémon Go. We also identified some challenges and concerns of playing Pokémon Go which borders on personal safety and security, circumvention of game design as well as privacy. Our findings led us to propose recommendation for augmented reality game design that will promote social interaction and physical activity. These include incorporating social game components to enhance players’ overall gaming experience, addressing Examining the impacts of Pokémon Go 3 game design circumvention by developing algorithms that will intelligently permit cheating or out rightly prevent cheating. We also recommended a more transparent and flexible permission requests to address privacy concerns. Examining the impacts of Pokémon Go 4
High fidelity of RecA-catalyzed recombination: a watchdog of genetic diversity

Homologous recombination plays a key role in generating genetic diversity, while maintaining protein functionality. The mechanisms by which RecA enables a single-stranded segment of DNA to recognize a homologous tract within a whole genome are poorly understood. The scale by which homology recognition takes place is of a few tens of base pairs, after which the quest for homology is over. To study the mechanism of homology recognition, RecA-promoted homologous recombination between short DNA oligomers with different degrees of heterology was studied in vitro, using fluorescence resonant energy transfer. RecA can detect single mismatches at the initial stages of recombination, and the efficiency of recombination is strongly dependent on the location and distribution of mismatches. Mismatches near the 5′ end of the incoming strand have a minute effect, whereas mismatches near the 3′ end hinder strand exchange dramatically. There is a characteristic DNA length above which the sensitivity to heterology decreases sharply. Experiments with competitor sequences with varying degrees of homology yield information about the process of homology search and synapse lifetime. The exquisite sensitivity to mismatches and the directionality in the exchange process support a mechanism for homology recognition that can be modeled as a kinetic proofreading cascade.
Application of Kalman Filtering and Wavelet Transform in DOAS

Differential optical absorption spectroscopy (DOAS) has become a widely used method to measure trace gases in the atmosphere. Concentration of trace gases is retrieved by fitting reference spectra to the atmospheric absorption spectra. There were large errors in retrieved results, when absorption structures were heavily overlapped and the noises were high. The novel method of combining Wavelet transform and Kalman filtering was developed and applied to DOAS data processing. The experiment results show the method can effectively correct overlapped spectra and reduce the noise. The novel method improves the accuracy of the DOAS system
Locating a compact odor source using a four-channel insect electroantennogram sensor

Here we demonstrate the feasibility of using an array of live insects to detect concentrated packets of odor and infer the location of an odor source (∼15 m away) using a backward Lagrangian dispersion model based on the Langevin equation. Bayesian inference allows uncertainty to be quantified, which is useful for robotic planning. The electroantennogram (EAG) is the biopotential developed between the tissue at the tip of an insect antenna and its base, which is due to the massed response of the olfactory receptor neurons to an odor stimulus. The EAG signal can carry tens of bits per second of information with a rise time as short as 12 ms (K A Justice 2005 J. Neurophiol. 93 2233–9). Here, instrumentation including a GPS with a digital compass and an ultrasonic 2D anemometer has been integrated with an EAG odor detection scheme, allowing the location of an odor source to be estimated by collecting data at several downwind locations. Bayesian inference in conjunction with a Lagrangian dispersion model, taking into account detection errors, has been implemented resulting in an estimate of the odor source location within 0.2 m of the actual location.
Heterogeneous feature code for expression recognition

The Heterogeneous Feature Code (HFC), a coding scheme based on both human and machine selected local features, is proposed for expression recognition. The HFC consists of two component codes, the Human Observable Code (HOC) and Boost Feature Code (BFC). The HOC is developed to capture the local deformation patches observable to humans when the face is showing an expression. Different expressions appear with a specific set of such patches with different deformation patterns at different locations, which are considered in the configuration of the HOC codewords. The BFC is built upon the mutually connected Haar-like features selected by a set of Adaboost classifiers followed by a multi-class SVM classifier. Unlike the HOC features, the BFC features can hardly be selected by human eyes. The HFC is probably the first code that combines human selected features and machine selected features, and proven effective for expression recognition. Performance evaluation on the Cohn-Kanade extension (CK+) database and the Japanese Female Facial Expression (JAFFE) shows that the HFC outperforms either HOC or BFC component code alone, and is competitive to the state-of-the-art.