Miracle-dz/UniFAR-FTUs huggingface dataset:
LY-CoV1404 potently neutralizes SARS-CoV-2 variants

are desperately needed, given that some of the existing therapeutic antibodies are less effective or ineffective against certain variants and the impact of variants on vaccine efficacy is still poorly understood. activity = acute 2. a IC50 values presented are geometric mean if multiple experiments were performed and a weighted geometric mean for the Wuhan strain (n=10) 95% CI values were included for variants tested only once. IC50 for ACE2-binding inhibition of Wuhan sequence is presented as the consensus estimate as of 2021 achieved across repeat experiments. b Fold shifts are calculated comparing to the in-experiment Wuhan control. The geometric mean of the fold changes is provided if multiple experiments were performed. For ACE2-binding inhibition, no inhibition represents <50% ACE2-binding inhibition with up to 3.75 μg/mL mAb. For the soluble assay, the IgG secreted by B-cells was captured on beads using the constant region. Binding to secreted IgG immobilized onto beads was subsequently assessed using soluble fluorescently labeled SARS-CoV-2 S or SARS-CoV-2 RBD antigen. Beads or cells were flowed onto microfluidic screening devices and incubated with single antibody-secreting cells, and mAb binding to cognate antigens was detected via a fluorescently labeled anti-human IgG secondary antibody, or soluble antigen labeled with fluorophore. Positive hits were identified using machine vision and recovered using automated robotics-based protocols. preparations by infection of VeroE6 cells (ATCC CRL-1586) with serially diluted virus followed by staining with an anti-luciferase antibody (Novus Cat # NB600-307PEATT594) and analysis by fluorescence-activated cell sorting using a Becton Dickinson LSRFortessaTM X-20. Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system. Relative luciferase reporter signal read-out was determined by luciferase assay (Promega Cat # E2650) of extracts from VeroE6 cells infected with serially diluted virus. Luciferase activity was measured on a PerkinElmer EnVision 2104 multilabel reader. concentration of mAb tested. experimental batch, data obtained duplicate or triplicate. potent vitro in vivo activity against SARS-CoV-2.
Design of the OPUS School Meal Study: A randomised controlled trial assessing the impact of serving school meals based on the New Nordic Diet

Introduction: Danish children consume too much sugar and not enough whole grain, fish, fruit, and vegetables. The Nordic region is rich in such foods with a strong health-promoting potential. We lack randomised controlled trials that investigate the developmental and health impact of serving school meals based on Nordic foods. Aim: This paper describes the rationale, design, study population, and potential implications of the Optimal well-being, development and health for Danish children through a healthy New Nordic Diet (OPUS) School Meal Study. Methods: In a cluster-randomised cross-over design, 1021 children from 3rd and 4th grades (8–11 years old) at nine Danish municipal schools were invited to participate. Classes were assigned to two 3-month periods with free school meals based on the New Nordic Diet (NND) or their usual packed lunch (control). Dietary intake, nutrient status, physical activity, cardiorespiratory fitness, sleep, growth, body composition, early metabolic and cardiovascular risk markers, illness, absence from school, wellbeing, cognitive function, social and cultural features, food acceptance, waste, and cost were assessed. Results: In total, 834 children (82% of those invited) participated. Although their parents were slightly better educated than the background population, children from various socioeconomic backgrounds were included. The proportion of overweight and obese children (14%) resembled that of earlier examinations of Danish school children. Drop out was 8.3%. Conclusions: A high inclusion rate and low drop out rate was achieved. This study will be the first to determine whether school meals based on the NND improve children’s diet, health, growth, cognitive performance, and early disease risk markers.
Hypostability in the default mode network and hyperstability in the frontoparietal cognitive network of dynamic functional architecture during rumination

The neural underpinnings of rumination can be characterized by its specific dynamic nature. Temporal stability is the stable and consistent representation of information by a distributed neural activity and connectivity pattern across brain regions. Although stability is a key feature of the brain‟s functional architecture, its profiles supporting rumination remain elusive. We characterized the stability of the whole-brain functional architecture during an induced, continuous rumination state and compared it with a well-constrained
Narrative reconstruction of mental illness as a work- stress- induced disorder: Processes, consequences and implications

Stress- induced mental illnesses have become the focus of increasing international attention, particularly in Japan since the 1990s, where judiciary cases and welfare- state initiatives established causal links between work stress and mental illness. However, how individuals retrospectively construct this causality remains a marginal topic in the literature. This ethnographic article explores the ways in which male workers seeking compensation for their condition (depression and adjustment disorder) reconstruct aetiology narratives and to what avail. This paper demonstrates two themes: (1) how objectivising stress (related to specific formats of worker compensation), no- faulting (dispensing with individual blame to view stress as pervasive in the workplace) and negotiating blame (seeking explanation in terms of individual psychology) construct a case, and (2) how narrative reconstruction functions as both a barrier to recovery and way of working towards recovery and collective
Velocity structure of upper ocean crust at Ocean Drilling Program Site 1256

We examine shipboard physical property measurements, wireline logs, and vertical seismic profiles (VSP) from Ocean Drilling Program/Integrated Ocean Drilling Program Hole 1256D in 15 Ma ocean crust formed at superfast spreading rates to investigate lateral and vertical variations in compressional velocity. In general, velocities from all methods agree. Porosity is inversely related to velocity in both the logging and laboratory data. We infer that microfracturing during drilling is minor in the upper 1 km of basement, probably due to connected pores and, thus, low effective stress. The closure of porosity to very low values coincides with the depth below which laboratory velocities diverge from logging velocities. We infer that porosity controls velocity in layer 2, lithostatic pressure controls the thickness of seismic layer 2, and the distribution of flow types determines seismic velocity in the upper 200 m of basement. In the sheeted dikes, changes in physical properties, mineralogy, and chemistry define clusters of dikes.
Risperidone-induced retrograde ejaculation.

TO THE EDITOR: Schizophrenic patients treated with antipsychotic drugs may suffer from a variety of sexually disabling side effects that can affect all domains of sexual activity. The most common such adverse effects in men are erectile dysfunction and retrograde ejaculation (1). Retrograde ejaculation has been mostly reported in association with typical antipsychotics, especially thioridazine (1); among the atypical antipsychotics, to our knowledge, only clozapine has been reported to cause retrograde ejaculation (2). We describe a schizophrenic patient with risperidone-induced ejaculatory failure that was compatible with retrograde ejaculation, both clinically and in the laboratory.
Thyroid Peroxidase Autoantibodies Obtained from Random Single Chain Fv Libraries Contain the Same Heavy/Light Chain Combinations as Occur in Vivo.

Three combinatorial libraries were constructed from unpurified, CD19+, and antithyroid peroxidase (anti-TPO) B cells extracted from thyroid tissue of Graves’ disease patients. Fifteen of the 41 randomly derived anti-TPO single chain variable region fragments (scFvs), showed VH1–3/Vλ1–51 or VH1–69/Vλ1–40 heavy/light chain pairing similar to that obtained with TPO-specific scFv derived from an in-cell library. One VH1–3/Vλ1–51 scFv, A16, showed exactly the same nucleotide sequence as in-cell scFv ICB7, demonstrating that in vivo rearrangement can be obtained from a random combinatorial library. The majority of the scFvs used a heavy chain gene derived from the VH1–3 gene segment, whereas the light chain gene segments used were more heterogeneous, with dominance of the Vκ1–39 and Vλ1–51 gene segments. The anti-TPO scFvs showed high affinities to TPO, with values between 0.77 and 12.3 nm, and defined seven antigenic regions on the TPO molecule. The anti-TPO fragments, particularly VH1–3/Vλ1–51 randomly associ.
Crustal rheology and seismicity in the Gibraltar Arc (western Mediterranean)

On the basis of the lithospheric structure of the Gibraltar Arc (western Mediterranean), we constrain depth distribution of crustal seismicity and active tectonics by means of rheological modeling. Crustal yield strength and depth of the brittle‐ductile transition zone (BDT) mimic the curvature of the arc with maximum depths of 12–9 km whereas in the Betics and Rif, BDT shallows eastward (to 6–5 km depth), oblique to crustal thickening. Most of the crustal seismicity (>60%) is placed within the brittle crust, decaying exponentially in the ductile crust. Active faults observed in surface geology, control the present topography of the Betics, connect in depth with scattered seismic swarms, and merge into the BDT. This horizon is interpreted as a decoupling horizon that conditions the mode of present‐day deformation partitioning. Below the BDT, low‐strength domains enable crustal flow under central Betics promoting stress rotation and topographic uplift.
Implied Dynamic Feedback of 3D IR Radiative Transfer on Simulated Cloud Fields

D. B. Mechem and Y. L. Kogan Cooperative Institute for Mesoscale Meteorological Studies University of Oklahoma Norman, Oklahoma M. Ovtchinnikov Pacific Northwest National Laboratory Richland, Washington K. F. Evans University of Colorado Boulder, Colorado A. B. Davis Los Alamos National Laboratory Los Alamos, New Mexico R. F. Cahalan National Aeronautics and Space Administration Goddard Space Flight Center Greenbelt, Maryland E. E. Takara and R. G. Ellingson Florida State University Tallahassee, Florida
Remote sensing and GIS analyses of the Strangways impact structure, Northern Territory

Remote sensing and GIS techniques play a substantial role for the identification of possible terrestrial impact structures, for mapping target-rock lithologies and deciphering the structural style of known craters. In this case study the lithological and structural characteristics of the highly eroded Proterozoic Strangways impact crater in the Northern Territory have been analysed on the basis of Landsat Enhanced Thematic Mapper satellite imagery, topographical data and airborne geophysical data. Regarding Landsat data, the calculation of basic statistical parameters and the optimum index factor has been found useful for a pre-selection of informative band combinations. By means of the analysis of multisensoral data, the distribution of crystalline basement rocks, siliciclastic target rocks of the Roper Group as well as post-impact deposits and deeper seated Proterozoic dykes can be detected. The original crater dimensions of the Strangways structure are carefully estimated at 26 – 29 km by combining the remote sensing data with the distribution of shatter cones localised in the field. The remote sensing/GIS approach of a geological interpretation based on multisensoral sources and combined fieldwork data can be successfully applied to other impact structures on earth, as well.
Diffusion Modeling Studies in Minerals and its Geological Applications

Diffusion in the solid state is a widespread process that has many attributes which allow it to function as a clock for measuring durations of a variety of terrestrial and planetary processes. The diffusion clock can measure the duration of very short (days) as well as long (millions of years) processes, and its functioning is independent of the age of the material. This makes it particularly valuable for studying short processes in the early history of earth and the solar system. Determining durations of the geological processes is one of the major goals in earth sciences. Records of incomplete diffusive equilibration preserved in minerals are suited for the purpose of unraveling timescales of a variety of geological processes. This review discusses the diffusion modeling studies in minerals and its geological applications.
Induced polarization of shaly sands

Equations have been developed that relate induced polarization (IP) in shaly sands to measurable petrophysical parameters. The induced-polarization process has been modeled in terms of two mechanisms: clay counterion displacement and membrane blockage. The resulting equations can be used to determine shaliness, brine conductivity, and oil saturation from in-phase and out-of-phase conductivities. Laboratory measurements have confirmed the IP dependence on these variables, as well as on temperature.
Ultrastructural analysis of dendritic spine necks reveals a continuum of spine morphologies: Models

Dendritic spines are membranous protrusions, with a bulbous head connected to the dendrite by a thin neck, and receive essentially all excitatory inputs in most mammalian neurons. Spines have a wide variety of morphologies that likely have a significant effect on their biochemical and electrical properties. The question of whether spines belong to distinct morphological or functional subtypes or constitute a continuum is still open. To discern this, it is important to measure spine necks objectively. Recent advances in electron microscopy enable automatic reconstructions of 3D spines with nanometer precision. Analyzing ultrastructural reconstructions from mouse neocortical neurons with computer vision algorithms, we demonstrate that the vast majority of spines can be rigorously separated into head and neck components. Analysis of the head and neck morphologies reveals a continuous distribution of parameters. The spine neck diameter, but not the neck length, was correlated with the head volume. Spines with larger head volumes often had a spine apparatus and pairs of spines in a postsynaptic cell contacted by the same axon had similar head volumes. Our data are consistent with a lack of morphological categories of spines and indicate that the morphologies of the spine neck and head are independently regulated. These results have repercussions for our understanding of the function of dendritic spines in neuronal circuits.
The Mediating Role of Perceived Disability

The aim of the study was to test whether acquired hearing loss (AHL)–related perceived disability mediates the association between AHL and psychological outcomes, including anger. Two-hundred ninety-seven consecutive outpatients with AHL assessed by pure tone average (PTA) loss completed the following: Hearing Handicap Inventory for Adults (HHIA), State-Trait Anger Expression Inventory–2 (STAXI-2), Brief Symptom Inventory (BSI), Diagnostic Criteria for Use in Psychosomatic Research (DCPR), and Social Functioning Questionnaire. In the sample, composed of 44.5% males with a mean age of 53.8 and a mean PTA of 30.7, AHL was associated to perceived hearing handicap, also correlating to all psychological measures except DCPR demoralization. Associations were stronger between the HHIA–Emotional Subscale, STAXI-2 State Anger and Feeling Angry, and BSI-Somatization, Interpersonal Sensitivity, Depression, and Psychoticism. Perceived disability predicted the presence of almost all psychosocial outcomes and confirms to be the most significant target of clinical action.
BRAIN TUMOR SEGMENTATION USING CONVOLUTIONAL NEURAL NETWORKS IN MRI IMAGES

Brain tumor segmentation is diagnosis and treatment of brain tumor. The diagnosing and treatment designing are needed to be more accurate and less time consuming. Magnetic resonance (MR) involves detecting any abnormal growth in any part of the body. This paper presenting a convolutional neural network (CNN) accommodates simpler 3x3 kernels to make whole process more reliable and progress in an appropriate rate. This tends to design deep architecture and further it eliminates positive effect occurred on over fitting; it would reduce weightage in neural network. By processing and analysis, the accuracy and time taken to complete it is verified experimentally.
Impact of Renal Impairment on Beta-Blocker Ef ﬁ cacy in Patients With Heart Failure

BACKGROUND Moderate and moderately severe renal impairment are common in patients with heart failure and reduced ejection fraction, but whether beta-blockers are effective is unclear, leading to underuse of life-saving therapy. OBJECTIVES This study sought to investigate patient prognosis and the ef ﬁ cacy of beta-blockers according to renal function using estimated glomerular ﬁ ltration rate (eGFR). METHODS Analysis of 16,740 individual patients with left ventricular ejection fraction < 50% from 10 double-blind, placebo-controlled trials was performed. The authors report all-cause mortality on an intention-to-treat basis, adjusted for baseline covariates and strati ﬁ ed by heart rhythm. RESULTS Median eGFR at baseline was 63 (interquartile range: 50 to 77) ml/min/1.73 m 2 ; 4,584 patients (27.4%) had eGFR 45 to 59 ml/min/1.73 m 2 , and 2,286 (13.7%) 30 to 44
Hepatic Scaling Factors for In Vitro-In Vivo Extrapolation (IVIVE) of Metabolic Drug Clearance in Patients with Colorectal Cancer with Liver Metastasis

In vitro-in vivo extrapolation (IVIVE) linked with physiologically based pharmacokinetic (PBPK) modelling is used to predict the fates of drugs in patients. Ideally, the IVIVE-PBPK models should incorporate “systems” information accounting for characteristics of the specific target population. There is a paucity of such scaling factors in cancer, particularly microsomal protein per gram of liver (MPPGL) and cytosolic protein per gram of liver (CPPGL). In this study, cancerous and histologically normal liver tissue from 16 patients with colorectal liver metastasis (CRLM) were fractionated to microsomes and cytosol. Protein content was measured in homogenates, microsomes and cytosol. The loss of microsomal protein during fractionation was accounted for using corrections based on NADPH cytochrome P450 reductase activity in different matrices. MPPGL was significantly lower in cancerous tissue (24.8 ± 9.8 mg/g) than histologically normal tissue (39.0 ± 13.8 mg/g). CPPGL in cancerous tissue was 42.1 ± 12.9 mg/g compared with 56.2 ± 16.9 mg/g in normal tissue. No correlations between demographics (sex, age and BMI) and MPPGL or CPPGL were apparent in the data. The generated scaling factors together with assumptions regarding the relative volumes of cancerous versus non-cancerous tissue were used to simulate plasma exposure of drugs with different extraction ratios. The PBPK simulations revealed a substantial difference in drug exposure (AUC), up to 3.3-fold, when using typical scaling factors (healthy population) instead of disease-related parameters in cancer population. These indicate the importance of using population-specific scalars in IVIVE-PBPK for different disease states. cancerous liver tissue from CRLM patients and compare the values with scalars from matched histologically normal tissue. The scalars were applied in PBPK simulations to predict in vivo hepatic clearance. This study highlights the importance of applying appropriate population-specific scalars for IVIVE of metabolic drug clearance in CRLM patients.
Further Characterization of Cys-Type and Ser-Type Anaerobic Sulfatase Maturating Enzymes Suggests a Commonality in Mechanism of Catalysis

The anaerobic sulfatase maturating enzyme from Clostridium perfringens (anSMEcpe) catalyzes the two-electron oxidation of a cysteinyl residue on a cognate protein to a formyglycyl residue (FGly) using a mechanism that involves organic radicals. The FGly residue plays a unique role as a cofactor in a class of enzymes termed arylsulfatases, which catalyze the hydrolysis of various organosulfate monoesters. anSMEcpe has been shown to be a member of the radical S adenosylmethionine (SAM) family of enzymes, [4Fe–4S] cluster–requiring proteins that use a 5’-deoxyadenosyl 5’-radical (5’-dA•) generated from a reductive cleavage of SAM to initiate radical-based catalysis. Herein, we show that anSMEcpe contains in addition to the [4Fe–4S] cluster harbored by all radical SAM (RS) enzymes, two additional [4Fe–4S] clusters, similar to the radical SAM protein AtsB, which catalyzes the two-electron oxidation of a seryl residue to a FGly residue. We show by size-exclusion chromatography that both AtsB and anSMEcpe are monomeric proteins, and site-directed mutagenesis studies on AtsB reveal that individual Cys → Ala substitutions at seven conserved positions result in insoluble protein, consistent with those residues acting as ligands to the two additional [4Fe–4S] clusters. Ala substitutions at an additional conserved Cys residue (C291 in AtsB; C276 in anSMEcpe) afford proteins that display intermediate behavior. These proteins exhibit reduced solubility and drastically reduced activity, behavior that is conspicuously similar to that of a critical Cys residue in BtrN, another radical SAM dehydrogenase T. L. , et al (2010) Biochemistry 49 3783–3785]. We also show that wild-type anSMEcpe acts on peptides containing other oxidizeable amino acids at the target position. Moreover, we show that the enzyme will convert threonyl peptides to the corresponding ketone product, and also allo -threonyl peptides, but with a significantly reduced efficiency, suggesting that the proS hydrogen atom of the normal cysteinyl substrate is stereoselectively removed during turnover. Lastly, we show that the electron generated during catalysis by AtsB and anSMEcpe can utilized for multiple turnovers, albeit through a reduced flavodoxin-mediated pathway. key role for this residue in catalysis. Last, we show that anSMEcpe is capable of converting Cys, Ser, and SeCys residues to FGly residues, as well as threonyl residues to the corresponding keto product, while the reaction of the corresponding allo -threonyl-containing substrate does not lead to substantial formation of the keto product. Collectively these results suggest that the key step in catalysis by anSMEs is abstraction of the 3- proS H• from the substrate by the 5’-dA• intermediate. Also discussed is the fate of the second electron removed from the target Ser or Cys residue during the two-electron oxidation.