Miracle-dz/UniFAR-FTUs huggingface dataset:
De Novo Biosynthetic Profiling of High Abundance Proteins in Cystic Fibrosis Lung Epithelial Cells*S

In previous studies with cystic fibrosis (CF) IB3-1 lung epithelial cells in culture, we identified 194 unique high abundance proteins by conventional two-dimensional gel electrophoresis and mass spectrometry (Pollard, H. B., Ji, X.-D., Jozwik, C. J., and Jacobowitz, D. M. (2005) High abundance protein profiling of cystic fibrosis lung epithelial cells. Proteomics 5, 2210–2226). In the present work we compared the IB3-1 cells with IB3-1/S9 daughter cells repaired by gene transfer with AAV-(wild type)CFTR. We report that gene transfer resulted in significant changes in silver stain intensity of only 20 of the 194 proteins. However, simultaneous measurement of de novo biosynthetic rates with [35S]methionine of all 194 proteins in both cell types resulted in the identification of an additional 31 CF-specific proteins. Of the 51 proteins identified by this hybrid approach, only six proteins changed similarly in both the mass and kinetics categories. This kinetic portion of the high abundance CF proteome, hidden from direct analysis of abundance, included proteins from transcription and signaling pathways such as NFκB, chaperones such as HSC70, cytoskeletal proteins, and others. Connectivity analysis indicated that ∼30% of the 51-member hybrid high abundance CF proteome interacts with the NFκB signaling pathway. In conclusion, measurement of biosynthetic rates on a global scale can be used to identify disease-specific differences within the high abundance cystic fibrosis proteome. Most of these kinetically defined proteins are unaffected in expression level when using conventional silver stain analysis. We anticipate that this novel hybrid approach to discovery of the high abundance CF proteome will find general application to other proteomic problems in biology and medicine.
The significance of skeletal-related events for the health-related quality of life of patients with metastatic prostate cancer.

BACKGROUND We examined the clinical relevance of skeletal-related events (SREs) for health state preferences, pain and health-related quality of life in patients with advanced prostate cancer and a history of bone metastases. PATIENTS AND METHODS Data were from a clinical trial of zoledronic acid versus placebo in the treatment of SREs associated with advanced prostate cancer metastatic to bone. Patients (n=248) were included if they experienced an SRE during the study. Outcome measures were assessed at fixed intervals. We used mixed-effects models to estimate changes in outcomes after each patient's first SRE. RESULTS There were clinically meaningful and statistically significant declines in physical well-being after: radiation and pathologic fractures; functional well-being after radiation; and emotional well-being after radiation and pathologic fractures. There also were meaningful and significant declines in preference and utility scores after radiation and fracture. Pain intensity declined after radiation, but not after other SREs; no other pain measure changed substantively. CONCLUSIONS SREs have important and significant effects on measures of health-related quality of life in men with prostate cancer. Treatments that prevent SREs may not demonstrate corresponding effects on outcomes if the effects of SREs occur between scheduled outcome assessments. Implications for trial design are discussed.
Taxi ‘sugar daddies’ and taxi queens: Male taxi driver attitudes regarding transactional relationships in the Western Cape, South Africa

Media reports are emerging on the phenomenon of young girls who travel with older mini-bus taxi drivers, and who are thought to have sex with the drivers in exchange for gifts and money. The extent to which such relationships might facilitate unsafe sexual practices and increased risks for both the men and the young women, often referred to as taxi queens, remains an important question in the light of the current challenges of HIV/AIDS in sub-Saharan Africa. However, very little research has been undertaken on this issue, especially regarding the perceptions and experiences of taxi drivers. Thus this paper aims to provide some preliminary findings on taxi drivers' attitudes and beliefs about taxi queens and their relationships with taxi drivers. A 22-item questionnaire was administered to 223 male taxi drivers in two regions in the Western Cape Province, South Africa. Taxi drivers in this study largely saw the relationship between taxi drivers and the young girls who ride with them as providing status for both the girls and drivers, and there seemed to be recognition of the transactional nature of the relationship between taxi drivers and taxi queens. The stigmatisation of young girls who ride with taxi drivers was evident. Drivers had knowledge and awareness of the risks of unsafe sex and supported condom use, although there appeared to be some uncertainty and confusion about the likelihood of HIV infection between drivers and girls. While taxi drivers recognised the role of alcohol in relationships with young girls, they seemed to deny that the abuse of drugs was common. The study highlights a number of key areas that need to be explored with men in the taxi industry, in order to address risk behaviours for both taxi drivers and the girls who ride with them.
Unemployment and risk of minor psychiatric disorder in young people: cross-sectional and longitudinal evidence

Synopsis Two age cohorts of young people were interviewed at times up to 2½ years after leaving school to investigate the association between unemployment and risk of minor psychiatric morbidity, as assessed by the General Health Questionnaire (GHQ), taking into account differences due to sex, ethnic group and educational qualifications. Cross-sectional data are presented on 2767 interviews. The psychometric properties of the GHQ justified the use of a total score. Although females had a higher risk of psychiatric morbidity, the strongest association was between unemployment and GHQ. The association between unemployment and GHQ scores was shown to be present after controlling for sex, ethnic group and educational qualification differences. Longitudinal analyses showed that the experience of unemployment was more likely to create increased symptoms, rather than the reverse.
Lycopene and Risk of Prostate Cancer

AbstractProstate cancer (PCa) is a common illness for aging males. Lycopene has been identified as an antioxidant agent with potential anticancer properties. Studies investigating the relation between lycopene and PCa risk have produced inconsistent results. This study aims to determine dietary lycopene consumption/circulating concentration and any potential dose–response associations with the risk of PCa. Eligible studies published in English up to April 10, 2014, were searched and identified from Pubmed, Sciencedirect Online, Wiley online library databases and hand searching. The STATA (version 12.0) was applied to process the dose–response meta-analysis. Random effects models were used to calculate pooled relative risks (RRs) and 95% confidence intervals (CIs) and to incorporate variation between studies. The linear and nonlinear dose–response relations were evaluated with data from categories of lycopene consumption/circulating concentrations. Twenty-six studies were included with 17,517 cases of PCa reported from 563,299 participants. Although inverse association between lycopene consumption and PCa risk was not found in all studies, there was a trend that with higher lycopene intake, there was reduced incidence of PCa (P = 0.078). Removal of one Chinese study in sensitivity analysis, or recalculation using data from only high-quality studies for subgroup analysis, indicated that higher lycopene consumption significantly lowered PCa risk. Furthermore, our dose–response meta-analysis demonstrated that higher lycopene consumption was linearly associated with a reduced risk of PCa with a threshold between 9 and 21 mg/day. Consistently, higher circulating lycopene levels significantly reduced the risk of PCa. Interestingly, the concentration of circulating lycopene between 2.17 and 85 &mgr;g/dL was linearly inversed with PCa risk whereas there was no linear association >85 &mgr;g/dL. In addition, greater efficacy for the circulating lycopene concentration on preventing PCa was found for studies with high quality, follow-up >10 years and where results were adjusted by the age or the body mass index. In conclusion, our novel data demonstrates that higher lycopene consumption/circulating concentration is associated with a lower risk of PCa. However, further studies are required to determine the mechanism by which lycopene reduces the risk of PCa and if there are other factors in tomato products that might potentially decrease PCa risk and progression.
Changing the programming paradigm for the embedded in the IoT domain

The enormous growth of the IoT with the simultaneous explosion of the interest on prototyping boards pushed the research to rethink also the way to making the embedded world easy accessible to several targets: designers, artists, but also engineer without a strong background in low level programming. In this paper the technical specifications of a comprehensive design suite for IoT prototyping are described and a possible implementation of a software suite for the design of smart objects is presented. The suite represents a design environment where different software tools are integrated in an integrated and user friendly framework.
Cascading XSL Filters for Content Selection in Multilingual Document Generation

Content selection is a key factor of any successful document generation system. This paper shows how a content selection algorithm has been implemented using an efficient combination of XML/XSL technology and the framework of RST for discourse modeling. The system generates multilingual documents adapted to user profiles in a learning environment for the web. This CourseViewGenerator applies simplified RST schemes to the elaboration of a master document in XML from which content segments are chosen to suit the user's needs. The personalisation of the document is achieved through the application of a sequence of filtering levels of text selection based on the user aspects given as input. These cascading filters are implemented in XSL.
Herschel CHESS discovery of the fossil cloud that gave birth to the Trapezium and Orion KL

Context. The Orion A molecular complex is a nearby (420 pc), very well studied stellar nursery that is believed to contain examples of triggered star formation. Aims. As part of the Herschel guaranteed time key programme CHESS, we present the discovery of a diffuse gas component in the foreground of the intermediate-mass protostar OMC-2 FIR 4, located in the Orion A region. Methods. Making use of the full HIFI spectrum of OMC-2 FIR 4 obtained in CHESS, we detected several ground-state lines from OH + ,H 2O + ,H F, and CH + , all of them seen in absorption against the dust continuum emission of the protostar’s envelope. We derived column densities for each species, as well as an upper limit to the column density of the undetected H3O + . In order to model and characterise the foreground cloud, we used the Meudon PDR code to run a homogeneous grid of models that spans a reasonable range of densities, visual extinctions, cosmic ray ionisation rates and far-ultraviolet (FUV) radiation fields, and studied the implications of adopting the Orion Nebula extinction properties instead of the standard interstellar medium ones. Results. The detected absorption lines peak at a velocity of 9 km s −1 , which is blue-shifted by 2 km s −1 with respect to the systemic velocity of OMC-2 FIR 4 (VLSR = 11. 4k m s −1 ). The results of our modelling indicate that the foreground cloud is composed of predominantly neutral diffuse gas (nH = 100 cm −3 ) and is heavily irradiated by an external source of FUV that most likely arises from the nearby Trapezium OB association. The cloud is 6 pc thick and bears many similarities with the so-called C + interface between Orion-KL and the Trapezium cluster, 2 pc south of OMC-2 FIR 4. Conclusions. We conclude that the foreground cloud we detected is an extension of the C + interface seen in the direction of Orion KL, and interpret it to be the remains of the parental cloud of OMC-1, which extends from OMC-1 up to OMC-2.
Does Overinclusiveness Equal Creativity

psychotic traits questionaire (STQ). BritishJournal ofClinical Psychology, 30, 311-324. Keefe, J. A. , & Magaro, P. A. (1980). Creativity and sdizophrenia: An equivalence of cognitive processing. Journal of Abnormal Psychology, 89, 390-398. Kelley, M. P., & Coursey, R. D. (1992). Factor structure of schizotypy scales. Personality and Inc vidual Differences, 13, 723-731. Magaro, P. A. (1981). The paranoid and the schizophrenic: The case for distinct cognitive style. Schizophrenia Bulletin, 7, 632-661. Muntaner, C. , Garcia-Sevilia, L, Fernandez, A. , & Torrubia, R. (1988). Personality dimensions, schizotypal and borderline personality traits andpsychosisproneness. Personality andIndividual Dfferences, 9,257-268. Prentky, R. (1980). Creativity and psychopathology: A neurocognitiveperspective. New York: Praeger. Prentky, R. (1989). Creativity and psychopathology: Gamboling at the seat of madness. In J. A. Glover, R. R. Ronning, & C. R. Reynolds (Eds.), Handbook of creativity (pp. 243-269). New York: Plenum. Raine, A. , & Allbutt, J. (1989). Factors of schizoid personality. British Journal of Clinical Psychology, 28, 31-40. Shimkunas, A. (1978). Hemispheric asymmetry and schizophrenic thoughtdisorder. In S. Schwartz (Ed.),Language and cognition in schizophrenia (pp. 193-235). Hillsdale, NJ: Lawrence Eribaum Associates, Inc. Silverman, J. (1967). Variations in cognitive control and psychophysiological defense in the schizophrenias. Psychosomatic Medicine, 29, 225-251. Venables, P. H. (1984). Cerebral mechanisms, autonomic responsivity and attention in schizophrenia. In G. W. Spaulding (Ed.), Theories of schizophrenia and psychosis (pp. 47-91). Lincoln: University of Nebraska Press. Zuckerman, M. (1989). Personality in thethirddimension: Apsychobiologicalapproach.PersonalityandIndividualDifferences, 10, 391-418.
Transarterial Onyx Embolization of Cranial Dural Arteriovenous Fistulas: Long-Term Follow-Up

BACKGROUND AND PURPOSE: Endovascular therapy with liquid embolic agents is a common treatment strategy for cranial dural arteriovenous fistulas. This study evaluated the long-term effectiveness of transarterial Onyx as the single embolic agent for curative embolization of noncavernous cranial dural arteriovenous fistulas. MATERIALS AND METHODS: We performed a retrospective review of 40 consecutive patients with 41 cranial dural arteriovenous fistulas treated between March 2006 and June 2012 by using transarterial Onyx embolization with intent to cure. The mean age was 57 years; one-third presented with intracranial hemorrhage. Most (85%) had cortical venous drainage. Once angiographic cure was achieved, long-term treatment effectiveness was assessed with DSA and clinical follow-up. RESULTS: Forty-nine embolization sessions were performed; 85% of cranial dural arteriovenous fistulas were treated in a single session. The immediate angiographic cure rate was 95%. The permanent neurologic complication rate was 2% (mild facial palsy). Thirty-five of the 38 patients with initial cure underwent short-term follow-up DSA (median, 4 months). The short-term recurrence rate was only 6% (2/35). All patients with occlusion at short-term DSA undergoing long-term DSA (median, 28 months) had durable occlusion. No patient with long-term clinical follow-up (total, 117 patient-years; median, 45 months) experienced hemorrhage. CONCLUSIONS: Transarterial embolization with Onyx as the single embolic agent results in durable long-term cure of noncavernous cranial dural arteriovenous fistulas. Recurrence rates are low on short-term follow-up, and all patients with angiographic occlusion on short-term DSA follow-up have experienced a durable long-term cure. Thus, angiographic cure should be defined at short-term follow-up angiography instead of at the end of the final embolization session. Finally, long-term DSA follow-up may not be necessary if occlusion is demonstrated on short-term angiographic follow-up.
Forecasting non-residents' monthly entries to Tunisia and accuracy comparison of time-series methods

The main objective of this article is to model and forecast non-residents' entries to the Tunisian tourist destination (North Africa). Five methods are applied, namely seasonal naïve 1, seasonal naïve 2, Holt–Winters seasonal multiplicative model, SARIMA and SARIMAX, incorporating the effects of political shocks Tunisia went through during the last 10 years. Although no clear-cut conclusions can be drawn and despite seasonal naïve 1 and Holt–Winters satisfactory performances, in-sample and out-of-sample forecast error calculations reveal that the SARIMAX is overall the most accurate method. On this basis, optimistic and pessimistic forecast scenarios are settled and managerial recommendations are proposed with the objective of improving Tunisian tourism policies.
LAGRANGIAN-CONIC RELAXATIONS, PART II: APPLICATIONS TO POLYNOMIAL OPTIMIZATION PROBLEMS

We present the moment cone (MC) relaxation and a hierarchy of sparse Lagrangian- SDP relaxations of polynomial optimization problems (POPs) using the unied framework established in Part I. The MC relaxation is derived for a POP of minimizing a polynomial subject to a nonconvex cone constraint and polynomial equality constraints. It is an ex- tension of the completely positive programming relaxation for QOPs. Under a copositivity condition, we characterize the equivalence of the optimal values between the POP and its MC relaxation. A hierarchy of sparse Lagrangian-SDP relaxations, which is parameterized by a positive integer ! called the relaxation order, is proposed for an equality constrained POP. It is obtained by combining a sparse variant of Lasserre's hierarchy of SDP relaxation of POPs and the basic idea behind the conic and Lagrangian-conic relaxations from the unied framework. We prove under a certain assumption that the optimal value of the Lagrangian-SDP relaxation with the Lagrangian multiplier and the relaxation order ! in the hierarchy converges to that of the POP as ! 1 and ! 1. The hierarchy of sparse Lagrangian-SDP relaxations is designed to be used in combination with the bisection and 1-dimensional Newton methods, which was proposed in Part I, for solving large-scale POPs eciently
In Vivo Dopamine Measurements in the Nucleus Accumbens After Nonanesthetic and Anesthetic Doses of Propofol in Rats

There is growing evidence that propofol acts on affective and reward processes. We designed this study to assess the effect of propofol on the concentration of dopamine in the nucleus accumbens, a main component of the mesolimbic system. The concentration of dopamine in the nucleus accumbens was assessed by using in vivo brain microdialysis in freely moving rats. A microdialysis probe was placed within guide cannulae previously placed during stereotaxic surgery. Fluid was perfused through the probe, and samples were collected every 20 min for measuring concentrations by high-pressure liquid chromatography. All rats served as their own controls and were randomized to four different doses of propofol, injected intraperitoneally: 0, 9, 60, or 100 mg/kg, according to a within design. Compared with the baseline value, dopamine concentration was decreased at the smallest dose of 9 mg/kg, whereas concentration was largely increased at the subanesthetic (60 mg/kg) and anesthetic (100 mg/kg) doses. This increase was of the same magnitude (+90%) for subanesthetic and anesthetic doses but was more prolonged at the anesthetic dose. Data show that only subanesthetic and anesthetic doses of propofol increase the concentration of dopamine in the nucleus accumbens, as previously described with drugs of potential abuse.
High-resolution methylation polymerase chain reaction for fragile X analysis: Evidence for novel FMR1 methylation patterns undetected in Southern blot analyses

Purpose: Fragile X syndrome is associated with the expansion of CGG trinucleotide repeats and subsequent methylation of the FMR1 gene. Molecular diagnosis of fragile X currently requires Southern blot analysis to assess methylation. This study describes the evaluation of a polymerase chain reaction-only workflow for the determination of methylation status across a broad range of FMR1 genotypes in male and female specimens.Methods: We evaluated a novel method that combines allele-specific methylation polymerase chain reaction and capillary electrophoresis with eight cell line and 80 clinical samples, including 39 full mutations. Methylation status was determined using a three-step workflow: (1) differential treatment of genomic DNA using a methylation-sensitive restriction enzyme; (2) polymerase chain reaction with two sets of dye-tagged primers; and (3) amplicon sizing by capillary electrophoresis. All samples were analyzed by both methylation polymerase chain reaction and Southern blot analysis.Results: FMR1 methylation status and CGG repeat sizing were accurately and reproducibly determined in a set of methylation controls and genomic DNA samples representing a spectrum of CGG repeat lengths and methylation states. Moreover, methylation polymerase chain reaction revealed allele-specific methylation patterns in premutation alleles that were unobtainable using Southern blot analysis.Conclusions: Methylation polymerase chain reaction enabled high throughput, high resolution, and semiquantitative methylation assessments of FMR1 alleles, as well as determinations of CGG repeat length. Results for all samples were concordant with corresponding Southern blot analyses. As a result, this study presents a polymerase chain reaction-based method for comprehensive FMR1 analysis. In addition, the identification of novel methylation mosaic patterns revealed after polymerase chain reaction and capillary electrophoresis may be relevant to several FMR1 disorders.
An online training-monitoring system to prevent nonfunctional overreaching.

PURPOSE This longitudinal case study evaluated the effectiveness of an online training-monitoring system to prevent nonfunctional overreaching (NFOR). METHODS A female master track and field athlete was followed by means of a daily online training diary (www.spartanova.com) and a weekly profile of mood state (POMS). The online diary consists of objective training data and subjective feelings reported on a 10-cm visual analog scale. Furthermore, parameters that quantify and summarize training and adaptation to training were calculated. The novelty consists in the inclusion of a specific measuring parameter tested to detect NFOR (OR score). RESULTS During track-season preparation, the athlete was facing some major personal changes, and extra training stress factors increased. Despite the fact that training load (TL) did not increase, the OR score showed a 222% and then a 997% increase compared with baseline. POMS showed a 167% increase in fatigue, a 38% decrease in vigor, a 32% increase in depression scores, and a total mood increase of 22%, with a 1-wk shift compared with the OR score. A 41% decrease in TL restored the OR score and POMS to baseline values within 10 d. CONCLUSION The results demonstrate that immediate feedback obtained by "warning signals" to both athletes and coaches, based on individual baseline data, seems an optimal predictor of FOR/NFOR.