Miracle-dz/UniFAR-FTUs huggingface dataset:
Assembly properties of altered beta-tubulin polypeptides containing disrupted autoregulatory domains

beta-Tubulin synthesis in eucaryotic cells is subject to control by an autoregulatory posttranscriptional mechanism in which the first four amino acids of the beta-tubulin polypeptide act either directly or indirectly to control the stability of beta-tubulin mRNA. To investigate the contribution of this amino-terminal domain to microtubule assembly and dynamics, we introduced a series of deletions encompassing amino acids 2 to 5 of a single mammalian beta-tubulin isotype, M beta 1. Constructs carrying such deletions were inserted into an expression vector, and the ability of the altered polypeptide to coassemble into microtubules was tested by using an anti-M beta 1-specific antibody. We show that the M beta 1 beta-tubulin polypeptide was competent for coassembly into microtubules in transient transfection experiments and in stably transfected cell lines when it lacked either amino acid 2 or amino acids 2 and 3. The capacity of these mutant beta-tubulins to coassemble into polymerized microtubules was only slightly diminished relative to that of unaltered beta-tubulin, and their expression did not influence the viability or growth properties of cell lines carrying these deletions. However, more extensive amino-terminal deletions either severely compromised or abolished the capacity for coassembly. In analogous experiments in which alterations were introduced into the amino-terminal domain of a mammalian alpha-tubulin isotype, M alpha 4, deletion of amino acid 2 did not affect the ability of the altered polypeptide to coassemble, although removal of additional amino-terminal residues essentially abolished the capacity for competent coassembly. The stability of the altered assembly-competent alpha- and beta-tubulin polypeptides was measured in pulse-chase experiments and found to be indistinguishable from the stability of the corresponding unaltered polypeptides. An assembly-competent M alpha 4 polypeptide carrying a deletion encompassing the 12 carboxy-terminal amino acids also had a half-life indistinguishable from that of the wild-type alpha-tubulin molecule. These data suggest that the universally conserved amino terminus of beta-tubulin acts largely in a regulatory role and that the carboxy-terminal domain of alpha-tubulin is not essential for coassembly in mammalian cells in vivo.
Effect of an on-site emergency physician in a rural emergency department at night.

INTRODUCTION The problem of emergency department (ED) overcrowding is an issue of some concern and staffing profile has been identified as a contributing factor. The aim of this study was to assess the effect of having an emergency physician on-site at night in a rural base hospital ED in terms of the ED length of stay, waiting times, admissions, specialist consultations, the use of diagnostic tests, and ED representations within 7 days. METHODS A retrospective analysis was performed of the ED database at Tamworth Base Hospital in rural New South Wales, Australia. A comparison was made between 125 patients seen when an emergency physician was in the department (Group A) and 117 patients seen when an emergency physician was not on site (Group B). RESULTS The mean ED length of stay was 48 min for Group A and 96 min for Group B. There were 15 admissions from Group A and 27 from Group B. There were significantly less pathology tests and consultations for the patients in Group A compared with Group B. There was no significant difference in waiting times or in the re-presentation rate between the two groups. CONCLUSIONS The presence of an on-site emergency physician resulted in a significantly shorter ED length of stay, lower admission rate, less initial pathology tests, and fewer telephone consultations.
Automated Transportation of Multiple Cell Types Using a Robot-Aided Cell Manipulation System With Holographic Optical Tweezers

Transferring multiple cell types with high precision and efficiency has become increasingly important for developing cell-based assays. In this study, an enabling technology is proposed for simultaneous automated transportation of multiple cell types utilizing a robot-aided cell manipulation system equipped with holographic optical tweezers. The dynamics of a trapped cell is initially analyzed. A control constraint is introduced to confine the offset of cells within the optical trap to prevent cells from escaping the trap during transportation. Unlike existing methods determining the critical offset through manual calibration for only a particular cell type, this proposed approach can automatically derive and apply the control constraint to multiple cell types with different radii. A controller is then developed for automated transportation of multiple cell types with different sizes in which exact values of model parameters, such as trapping stiffness and drag coefficient, are not required. Experiments are finally performed on the transportation of yeast cells and osteoblast-like MC3T3-E1 cells to demonstrate the effectiveness of the proposed approach.
Metabolic Disorders in the Transition Period Indicate that the Dairy Cows’ Ability to Adapt is Overstressed

Simple Summary Metabolic disorders are a key problem in the transition period of dairy cows and often appear before the onset of further health problems. Problems derive from difficulties animals have to adapt to large variations and disturbances occurring both outside and inside the organism. A lack of success in solving these issues may be due to predominant approaches in farm management and agricultural science, dealing with such disorders as merely negative side effects. Instead, a successful adaptation of animals to their living conditions should be seen as an important end in itself. Both farm management and agricultural sciences should support animals in their ability to cope with nutritional and metabolic challenges by employing a functional and result-driven approach. Abstract Metabolic disorders are a key problem in the transition period of dairy cows and often appear before the onset of further health problems. They mainly derive from difficulties the animals have in adapting to changes and disturbances occurring both outside and inside the organisms and due to varying gaps between nutrient supply and demand. Adaptation is a functional and target-oriented process involving the whole organism and thus cannot be narrowed down to single factors. Most problems which challenge the organisms can be solved in a number of different ways. To understand the mechanisms of adaptation, the interconnectedness of variables and the nutrient flow within a metabolic network need to be considered. Metabolic disorders indicate an overstressed ability to balance input, partitioning and output variables. Dairy cows will more easily succeed in adapting and in avoiding dysfunctional processes in the transition period when the gap between nutrient and energy demands and their supply is restricted. Dairy farms vary widely in relation to the living conditions of the animals. The complexity of nutritional and metabolic processes and their large variations on various scales contradict any attempts to predict the outcome of animals’ adaptation in a farm specific situation. Any attempts to reduce the prevalence of metabolic disorders and associated production diseases should rely on continuous and comprehensive monitoring with appropriate indicators on the farm level. Furthermore, low levels of disorders and diseases should be seen as a further significant goal which carries weight in addition to productivity goals. In the long run, low disease levels can only be expected when farmers realize that they can gain a competitive advantage over competitors with higher levels of disease.
Microbial community structure and function on sinking particles in the North Pacific Subtropical Gyre

Sinking particles mediate the transport of carbon and energy to the deep-sea, yet the specific microbes associated with sedimenting particles in the ocean's interior remain largely uncharacterized. In this study, we used particle interceptor traps (PITs) to assess the nature of particle-associated microbial communities collected at a variety of depths in the North Pacific Subtropical Gyre. Comparative metagenomics was used to assess differences in microbial taxa and functional gene repertoires in PITs containing a preservative (poisoned traps) compared to preservative-free traps where growth was allowed to continue in situ (live traps). Live trap microbial communities shared taxonomic and functional similarities with bacteria previously reported to be enriched in dissolved organic matter (DOM) microcosms (e.g., Alteromonas and Methylophaga), in addition to other particle and eukaryote-associated bacteria (e.g., Flavobacteriales and Pseudoalteromonas). Poisoned trap microbial assemblages were enriched in Vibrio and Campylobacterales likely associated with eukaryotic surfaces and intestinal tracts as symbionts, pathogens, or saprophytes. The functional gene content of microbial assemblages in poisoned traps included a variety of genes involved in virulence, anaerobic metabolism, attachment to chitinaceaous surfaces, and chitin degradation. The presence of chitinaceaous surfaces was also accompanied by the co-existence of bacteria which encoded the capacity to attach to, transport and metabolize chitin and its derivatives. Distinctly different microbial assemblages predominated in live traps, which were largely represented by copiotrophs and eukaryote-associated bacterial communities. Predominant sediment trap-assocaited eukaryotic phyla included Dinoflagellata, Metazoa (mostly copepods), Protalveolata, Retaria, and Stramenopiles. These data indicate the central role of eukaryotic taxa in structuring sinking particle microbial assemblages, as well as the rapid responses of indigenous microbial species in the degradation of marine particulate organic matter (POM) in situ in the ocean's interior.
Timing in Solo and Duet Piano Performances

Perhaps some of the most refined forms of timing arise in musical performance, particularly in the coordination between musicians playing together. Studies of timing in solo and duet piano performances are described, in which the musicians gave repeat performances of the music. In both solo and duet performances there was expressive use of timing, modulating the tempo of the music and the phase relationship between the voices, and the expressive forms were similar in successive performances of the piece. There was also evidence of separate timing control of the metre and of the production of notes and rests. Thus timing in musical performance is best modelled by assuming two levels of timekeeper, one pacing the metre and the other contained in the movement trajectories of note production, computed by motor procedure in relation to the metre. It is argued that expressive forms are derived from an interpretation of the music rather than memorized; and that coordination between voices in the music is achieved at the level of the metre.
Measuring continuous baseline covariate imbalances in clinical trial data

This paper presents and compares several methods of measuring continuous baseline covariate imbalance in clinical trial data. Simulations illustrate that though the t-test is an inappropriate method of assessing continuous baseline covariate imbalance, the test statistic itself is a robust measure in capturing imbalance in continuous covariate distributions. Guidelines to assess effects of imbalance on bias, type I error rate and power for hypothesis test for treatment effect on continuous outcomes are presented, and the benefit of covariate-adjusted analysis (ANCOVA) is also illustrated.
Memory in our body: Thick participation and the translation of kinesthetic experience

Anthropologists studying dance, sports, or other kinesthetic cultures may face the difficulty of rendering into analytic discourse those bodily practices consultants do not express verbally. Through thick participation, we may use our bodies to acquire shared cultural knowledge, but then we must translate our somatic experience into words. Encoding kinesthetic details, describing new sensations, and narrating physical training episodes are three different ways of writing about participatory experiences in the field to yield data that can then be compared with observations and life histories of consultant practitioners. Each approach provides distinctive avenues for investigating coherence across multiple bodies inhabiting the same kinesthetic culture, in this case, a Chinese Indonesian self-defense and health movement system known as White Crane Silat. [body, kinesthetic culture, thick participation, ethnography, silat, martial art]
[The megaloblastic anemias].

The inclusion in this volume of over 600 references indicates the thoroughness with which Dr. Herbert has reviewed the literature on the megaloblastic anemias. A student of these disorders, the author has digested and evaluated the great variety of studies that have been made in this field and has crystallized this information effectively. This is a useful and thoughtful monograph.
Impact of Insulin Deficiency on Glucose Fluxes and Muscle Glucose Metabolism During Exercise

Exercise in the insulin-deficient diabetic state is characterized by a further increase in elevated circulating glucose and NEFA levels and by excessive counterregulatory hormone levels. The aim of this study was to distinguish the direct glucoregulatory effects of insulinopenia during exercise from the indirect effects that result from the metabolic and hormonal environment that accompanies insulin deficiency. For this purpose, dogs underwent 90 min of treadmill exercise during SRIF infusion with (SRIF+INS, n = 8) or without (SRIF-INS, n = 6) intraportal insulin replacement. Glucagon was not replaced, thus allowing assessment of the direct effect of insulinopenia at the liver independent of the potentiation of glucagon action. Glucose was infused to maintain euglycemia. Hepatic glucose production (Ra); glucose utilization (Rd); and LGIcU, LGIcE, and LGIcO were assessed with tracers ([3H]glucose, [14C]glucose) and arteriovenous differences. With exercise, insulin fell from 66 ± 6 to 42 ± 6 pM in the SRIF+INS group, and was undetectable in the SRIF-INS group. Plasma glucose was 6.33 ± 0.38 and 6.26 ± 0.30 mM at rest in the SRIF+INS and SRIF-INS groups, respectively, and was unchanged with exercise. Ra rose from 7.5 ± 2.3 to 16.5 ± 2.2 μmol · kg−1 · min−1 and 9.1 ± 2.0 to 31.4 ± 3.9 μmol · kg−1 · min−1 with exercise in the SRIF+INS and SRIF-INS groups, whereas Rd rose from 19.5 ± 2.0 to 46.8 ± 3.9 μmol · kg−1 · min−1 and 15.1 ± 1.8 to 29.9 ± 3.3 μmol · kg−1 · min−1. LGIcU rose from 36 ± 9 to 112 ± 25 μmol/min and 1 5 ± 4 t o 59 ± 13 μmol/min and LGIcO rose from 5 ± 2 to 61 ± 12 μmol/min and 5 ± 3 to 32 ± 9 μmol/min with exercise in the SRIF+INS and SRIF-INS groups, respectively. Arterial levels and limb balances of NEFAs and glycerol were similar in the two groups. In summary, during exercise: 1) marked insulinopenia attenuates the increases in muscle glucose uptake and oxidation by ∼50%, independent of changes in circulating metabolic substrate levels; 2) substantial increases in muscle glucose uptake and oxidation are, however, still present even in the absence of detectable insulin levels; and 3) insulinopenia facilitates the increase in Ra, independent of the potentiation of basal glucagon action. In conclusion, marked insulinopenia contributes directly to the exacerbation of glucoregulation during exercise in the diabetic state by limiting the rises in glucose uptake and metabolism and by enhancing hepatic glucose production.
Bacterial Diversity and Mycotoxin Reduction During Maize Fermentation (Steeping) for Ogi Production

Bacterial diversity and community structure of two maize varieties (white and yellow) during fermentation/steeping for ogi production, and the influence of spontaneous fermentation on mycotoxin reduction in the gruel were studied. A total of 142 bacterial isolates obtained at 24–96 h intervals were preliminarily identified by conventional microbiological methods while 60 selected isolates were clustered into 39 OTUs consisting of 15 species, 10 genera, and 3 phyla by 16S rRNA sequence analysis. Lactic acid bacteria constituted about 63% of all isolated bacteria and the genus Pediococcus dominated (white maize = 84.8%; yellow maize = 74.4%). Pediococcus acidilactici and Lactobacillus paraplantarum were found at all steeping intervals of white and yellow maize, respectively, while P. claussenii was present only at the climax stage of steeping white maize. In both maize varieties, P. pentosaceus was found at 24–72 h. Mycotoxin concentrations (μg/kg) in the unsteeped grains were: white maize (aflatoxin B1 = 0.60; citrinin = 85.8; cyclopiazonic acid = 23.5; fumonisins (B1/B2/B3) = 68.4–483; zearalenone = 3.3) and yellow maize (aflatoxins (B1/B2/M1) = 22.7–513; citrinin = 16,800; cyclopiazonic acid = 247; fumonisins (B1/B2/B3) = 252–1,586; zearalenone = 205). Mycotoxins in both maize varieties were significantly (p < 0.05) reduced across steeping periods. This study reports for the first time: (a) the association of L. paraplantarum, P. acidilactici, and P. claussenii with ogi production from maize, (b) citrinin occurrence in Nigerian maize and ogi, and (c) aflatoxin M1, citrinin and cyclopiazonic acid degradation/loss due to fermentation in traditional cereal-based fermented food.
Online spelling correction for query completion

In this paper, we study the problem of online spelling correction for query completions. Misspelling is a common phenomenon among search engines queries. In order to help users effectively express their information needs, mechanisms for automatically correcting misspelled queries are required. Online spelling correction aims to provide spell corrected completion suggestions as a query is incrementally entered. As latency is crucial to the utility of the suggestions, such an algorithm needs to be not only accurate, but also efficient. To tackle this problem, we propose and study a generative model for input queries, based on a noisy channel transformation of the intended queries. Utilizing spelling correction pairs, we train a Markov n-gram transformation model that captures user spelling behavior in an unsupervised fashion. To find the top spell-corrected completion suggestions in real-time, we adapt the A* search algorithm with various pruning heuristics to dynamically expand the search space efficiently. Evaluation of the proposed methods demonstrates a substantial increase in the effectiveness of online spelling correction over existing techniques.
Shortest paths for finned, winged, legged, and wheeled vehicles with side-looking sensors

Sensor-guided robotic locomotion in different media often takes inspiration from natural examples, e.g. from fishes, birds, and humans for underwater, aerial, or walking robots, respectively. Interestingly, several naturalistic observations show that even very different species exhibit some similarities in their locomotion patterns: a notable one perhaps being the spiraling nature of paths that in some cases can be observed in sensory-guided tasks. As often conjectured in naturalistic studies, a common optimality principle may underpin such motion behaviors. We show in a robotics framework that spiraling motions appear in the solution of the problem of minimum path length. In particular, we study optimal paths for a simple model of finned, winged, and legged robotic vehicles, under different constraints on the field-of-view of the available sensory inputs. After showing that logarithmic spirals are indeed extremals of a sensory-constrained shortest-path problem, we provide a complete synthesis of the optimal control for different robotic vehicles. Application of these results to robotics can reduce the length of paths to be followed by underwater, aerial, or legged robots to reach targets in their environment. The work also provides some interesting, although preliminary, insights into how sensory field-of-view limitations may influence motion patterns in natural systems.
Trials of dietary intervention and impact on key features in overweight / obese women with polycystic Study Patients Duration Intervention Weight loss

Lisa J. Moran, Ph.D., Renato Pasquali, M.D., Helena J. Teede, Ph.D., Kathleen M. Hoeger, M.D., and Robert J. Norman, M.D. a The Jean Hailes Foundation for Women’s Health, Monash Institute of Health Services Research, Monash University, Melbourne, Victoria, Australia; b Division of Endocrinology, Department of Internal Medicine, S.Orsola-Malpighi Hospital, University Alma Mater Studiorum, Bologna, Italy; c Department of Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Rochester, New York; and d The Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, University of Adelaide, Adelaide, South Australia, Australia
A Multiechelon Repairable Item Inventory System with Lateral Transshipment and a General Repair Time Distribution

This paper discusses the determination of the spare inventory level for a multiechelon repairable item inventory system, which has several bases and a central depot with emergency lateral transshipment capability. Previous research is extended by removing a restrictive assumption on the repair time distribution. A mathematical model that allows a general repair time distribution, as well as an algorithm to find a solution of the model, is developed. Thus, the main focus of this study is to improve the accuracy of previous models and to estimate the gain in accuracy from use of the current methodology. Computational experiments are performed to estimate the accuracy improvement and to determine the managerial implications of the results.
Cryptococcus neoformans CAP59 (or Cap59p) Is Involved in the Extracellular Trafficking of Capsular Glucuronoxylomannan

ABSTRACT Several genes are essential for Cryptococcus neoformans capsule synthesis, but their functions are unknown. We examined the localization of glucuronoxylomannan (GXM) in strain B-3501 and in cap59 mutants B-4131 and C536. Wild-type strain B-3501 showed a visible capsule by India ink staining and immunofluorescence with anticapsular monoclonal antibodies (MAbs) 12A1 and 18B7. B-4131, a mutant containing a missense mutation in CAP59, showed no capsule by India ink staining but revealed the presence of capsular polysaccharide on the cell surface by immunofluorescence. The cap59 gene deletion mutant (C536), however, did not show a capsule by either India ink staining or immunofluorescence. Analysis of cell lysates for GXM by enzyme-linked immunosorbent assay revealed GXM in C536 samples. Furthermore, the epitopes recognized by MAbs 12A1, 2D10, 13F1, and 18B7 were each detected in the cytoplasm of all strains by immunogold electron microscopy, although there were differences in location consistent with differences in epitope synthesis and/or transport. In addition, the cells of B-3501 and B-4131, but not those of the cap59 deletant, assimilated raffinose or urea. Hence, the missense mutation of CAP59 in B-4131 partially hampered the trafficking of GXM but allowed the secretion of enzymes involved in hydrolysis of raffinose or urea. Furthermore, the cell diameter and volume for strain C536 are higher than those for strain B-3501 or B-4131 and may suggest the accumulation of cellular material in the cytoplasm. Our results suggest that CAP59 is involved in capsule synthesis by participating in the process of GXM (polysaccharide) export.