Miracle-dz/UniFAR-FTUs huggingface dataset:
Heat can erase epigenetic marks of vernalization in Arabidopsis

Vernalization establishes a memory of winter that must be maintained for weeks or months in order to promote flowering the following spring. The stability of the vernalized state varies among plant species and depends on the duration of cold exposure. In Arabidopsis thaliana, winter leads to epigenetic silencing of the floral repressor gene FLOWERING LOCUS C (FLC) and the duration of cold is measured through the dynamics of chromatin modifications during and after cold. The growing conditions encountered post-vernalization are thus critical for the maintenance of the vernalized state. We reported that high temperature leads to devernalization and, consistently, to FLC reactivation in Arabidopsis seedlings. Here we show that the repressive epigenetic mark H3K27me3 decreases at the FLC locus when vernalized seedlings are grown at 30°C, unless they were first exposed to a stabilizing period at 20°C. Ambient temperature thus controls the epigenetic memory of winter.
Business flight simulators in the framework of information strategy

The present trend of information technology makes it possible to simulate economic process courses and results very well. The simulation by itself does not replace reality but substantially improves quality. Company information systems often abound in data and information overloading. Sometimes nobody uses them and the information system does not work as a whole. For better understanding and company integration we look for more powerful tools for decision making in real time. Business flight simulators can provide company information strategy with suitable tools for decision making. Business flight simulators are able to create different “what-if” scenarios and analysis. Results from company information systems, data warehouses and other unstructured sources (texts, spreadsheet programs, market behavior, economic index of other companies, etc.) could be used as data. All summary indexes, on the basis of the data history, are able to create predictions and help future progress. The level of strategic planning and production control is very closely linked with other strategic areas such as marketing, trade or economy. The access to real data is one of the advantages which can be supported by simulators and can help managers make a decision and see the future impact these decisions may have. The simulator can not be isolated but it has to be integrated into the company's information system. Business flight simulators provide a kind of virtual reality.
Asymmetric Division and Differential Gene Expression during a Bacterial Developmental Program Requires DivIVA

Sporulation in the bacterium Bacillus subtilis is a developmental program in which a progenitor cell differentiates into two different cell types, the smaller of which eventually becomes a dormant cell called a spore. The process begins with an asymmetric cell division event, followed by the activation of a transcription factor, σF, specifically in the smaller cell. Here, we show that the structural protein DivIVA localizes to the polar septum during sporulation and is required for asymmetric division and the compartment-specific activation of σF. Both events are known to require a protein called SpoIIE, which also localizes to the polar septum. We show that DivIVA copurifies with SpoIIE and that DivIVA may anchor SpoIIE briefly to the assembling polar septum before SpoIIE is subsequently released into the forespore membrane and recaptured at the polar septum. Finally, using super-resolution microscopy, we demonstrate that DivIVA and SpoIIE ultimately display a biased localization on the side of the polar septum that faces the smaller compartment in which σF is activated.
Regional and seasonal patterns of litterfall in tropical South America

Abstract. The production of aboveground soft tissue represents an important share of total net primary production in tropical rain forests. Here we draw from a large number of published and unpublished datasets (n=81 sites) to assess the determinants of litterfall variation across South American tropical forests. We show that across old-growth tropical rainforests, litterfall averages 8.61±1.91 Mg ha−1 yr−1 (mean ± standard deviation, in dry mass units). Secondary forests have a lower annual litterfall than old-growth tropical forests with a mean of 8.01±3.41 Mg ha−1 yr−1. Annual litterfall shows no significant variation with total annual rainfall, either globally or within forest types. It does not vary consistently with soil type, except in the poorest soils (white sand soils), where litterfall is significantly lower than in other soil types (5.42±1.91 Mg ha−1 yr−1). We also study the determinants of litterfall seasonality, and find that it does not depend on annual rainfall or on soil type. However, litterfall seasonality is significantly positively correlated with rainfall seasonality. Finally, we assess how much carbon is stored in reproductive organs relative to photosynthetic organs. Mean leaf fall is 5.74±1.83 Mg ha−1 yr−1 (71% of total litterfall). Mean allocation into reproductive organs is 0.69±0.40 Mg ha−1 yr−1 (9% of total litterfall). The investment into reproductive organs divided by leaf litterfall increases with soil fertility, suggesting that on poor soils, the allocation to photosynthetic organs is prioritized over that to reproduction. Finally, we discuss the ecological and biogeochemical implications of these results.
Give me a reason: hedonic product choice and justification

Recently, researchers and practitioners of Human-Computer Interaction started to distinguish instrumen-tal, task-related, pragmatic quality aspects (i.e., use-fulness, usability) of interactive products from non-instrumental, self-referential, hedonic quality aspects (e.g., beauty, novelty). Although both qualities are ap-preciated while using a product, hedonic quality tends to be downplayed in the moment of product choice. We suggest and test the idea that this is the consequence of an increased experienced pressure to justify hedonic choices and according expenditures.
High-Aspect-Ratio SOI Vibrating Micromechanical Resonators and Filters

This paper presents a review of single crystal silicon bulk acoustic wave resonators and filters. Silicon-on-insulator (SOI) substrates are utilized to implement high quality factor (Q) integrated silicon resonators with capacitive and/or piezoelectric transducers. These resonators cover a wide range of frequencies including HF, VHF and UHF
The Heart and the Head: On Choosing Experiences Intuitively and Possessions Deliberatively

An enduring question in the field of judgment and decision making is when people are likely to choose on the basis of intuition and when they are likely to pursue a more deliberative decision strategy. Here, we attempt to shed light on that question by examining whether people tend to weight intuition more heavily when making experiential purchases, and to weight deliberation more heavily when making material purchases. Results from seven studies indicate that they do. In Study 1 (and a replication), participants expressed an explicit preference for choosing experiential purchases intuitively and material purchases analytically. In Study 2 (and a replication), participants anticipated experiencing more regret after going against reason for material purchases and going against intuition for experiential purchases. Participants in Study 3 who were asked to think about an experiential purchase wanted to see the relevant information presented by alternative, which facilitates intuitive/holistic processing, more than did those who were asked to consider a material purchase. In two additional studies, participants who were induced to think intuitively chose experiential purchases more often (Study 4) and reported a higher willingness to pay for them (Study 5) compared with participants induced to think analytically. Copyright © 2016 John Wiley & Sons, Ltd.
Porphyromonas gingivalis fimbriae

Marginal periodontitis is not a homogeneous disease but is rather influenced by an intricate set of host susceptibility differences as well as diversities in virulence among the harbored organisms. It is likely that clonal heterogeneity of subpopulations with both high and low levels of pathogenicity exists among organisms harbored by individuals with negligible, slight, or even severe periodontal destruction. Therefore, specific virulent clones of periodontal pathogens may cause advanced and/or aggressive periodontitis. Porphyromonas gingivalis is a predominant periodontal pathogen that expresses a number of potential virulence factors involved in the pathogenesis of periodontitis, and accumulated evidence shows that its expression of heterogenic virulence properties is dependent on clonal diversity. Fimbriae are considered to be critical factors that mediate bacterial interactions with and invasion of host tissues, with P. gingivalis shown to express two distinct fimbria-molecules, long and short fimbriae, on the cell surface, both of which seem to be involved in development of periodontitis. Long fimbriae are classified into six types (I to V and Ib) based on the diversity of fimA genes encoding FimA (a subunit of long fimbriae). Studies of clones with type II fimA have revealed their significantly greater adhesive and invasive capabilities as compared to other fimA type clones. Long and short fimbriae induce various cytokine expressions such as IL-1α, IL-β, IL-6, and TNF-α, which result in alveolar bone resorption. Although the clonal diversity of short fimbriae is unclear, distinct short fimbria-molecules have been found in different strains. These fimbriae variations likely influence the development of periodontal disease.
Phosphorylation of myosin light chain during capping of mouse T- lymphoma cells

Colchicine induces the clustering of at least three different T- lymphoma surface antigens (T200, Thy-1, and gp 69/71) into a cap structure in the absence of any external ligand. In addition, colchicine induces the intracellular accumulation of actin and myosin directly beneath the surface cap structure. We have discovered that myosin molecules (both heavy and light chains) are closely associated with the plasma membrane of T-lymphoma cells. Most importantly, we have found that the 20,000-dalton light chain of lymphocyte myosin is both phosphorylated and preferentially accumulated in the plasma membrane of colchicine-induced capped cells. It is proposed that myosin light chain is directly involved in the activation of membrane-associated actomyosin required for the collection of surface proteins into a cap structure (analogous to muscle cell sliding filament contraction).
Expression-Invariant Face Recognition with Expression Classification

Face recognition is one of the most intensively studied topics in computer vision and pattern recognition. Facial expression, which changes face geometry, usually has an adverse effect on the performance of a face recognition system. On the other hand, face geometry is a useful cue for recognition. Taking these into account, we utilize the idea of separating geometry and texture information in a face image and model the two types of information by projecting them into separate PCA spaces which are specially designed to capture the distinctive features among different individuals. Subsequently, the texture and geometry attributes are re-combined to form a classifier which is capable of recognizing faces with different expressions. Finally, by studying face geometry, we are able to determine which type of facial expression has been carried out, thus build an expression classifier. Numerical validations of the proposed method are given.
Glaukos iStent inject® Trabecular Micro-Bypass Implantation Associated with Cataract Surgery in Patients with Coexisting Cataract and Open-Angle Glaucoma or Ocular Hypertension: A Long-Term Study

Purpose. To evaluate the long-term efficacy and safety of the iStent inject device (Glaukos Corporation, Laguna Hills, CA) combined with phacoemulsification in patients with coexistent cataract and open-angle glaucoma or ocular hypertension (OHT). Methods. A prospective, uncontrolled, nonrandomized, interventional case series study was conducted in patients with both mild or moderate open-angle glaucoma or OHT and cataract. Patients underwent cataract surgery along with the implant of two iStent inject devices. Outcome measures were intraocular pressure (IOP), topical hypotensive medications required, and best-corrected visual acuity (BCVA). Results. 20 patients were enrolled. Mean follow-up was 47.4 ± 18.46 months. Mean baseline IOP was 19.95 ± 3.71 mmHg with medication and 26 ± 3.11 mmHg after washout. Mean end-follow-up IOP was 16.25 ± 1.99 mmHg, representing an IOP decrease of 36.92%, 9.74 ± 3.14 mmHg (P < 0.001), from baseline washout IOP. The mean number of medications was significantly reduced from 1.3 ± 0.66 to 0.75 ± 0.79 (P = 0.017). 45% of patients were medication-free by the end of follow-up. Mean log⁡MAR BCVA improved significantly from 0.42 ± 0.16 to 0.18 ± 0.16 (P < 0.001). No complications of surgery were observed. Conclusion. The iStent inject device combined with cataract surgery served to significantly reduce both IOP and medication use in the long term in patients with coexistent open-angle glaucoma or ocular hypertension (OHT) and cataract.
Tutorial in Molecular and Cellular Biology Cell Calcium, Oncogenes, and Hypertrophy

The cellular mechanisms of cardiac hypertrophy remain unclear despite tantalizing clues gleaned from a variety of experimental approaches. Here we examine the hypothesis that an increase in cytosolic free Ca concentration ([Ca],) triggers the expression of protooncogenes, which in turn direct the characteristic increase in protein synthesis. New results from perfused ferret hearts are presented demonstrating that [Ca], increases as a direct consequence of an elevation in perfusion pressure. It therefore seems plausible that [Ca], constitutes the crucial link between the initial stimulus for hypertensive hypertrophy (elevated perfusion pressure) and the secondary alterations in gene expression. Nevertheless, further investigation will be required to establish whether changes in [Ca], are necessary or sufficient to stimulate myocardial cell growth. {Hypertension 1990;15:652-658)
The Structural Determinants of Insulin-Like Peptide 3 Activity

Insulin-like peptide 3 (INSL3) is a hormone and/or paracrine factor which is a member of the relaxin peptide family. It has key roles as a fertility regulator in both males and females. The receptor for INSL3 is the leucine rich repeat (LRR) containing G-protein coupled receptor 8 (LGR8) which is now known as relaxin family peptide receptor 2 (RXFP2). Receptor activation by INSL3 involves binding to the LRRs in the large ectodomain of RXFP2 by residues within the B-chain of INSL3 as well as an interaction with the transmembrane exoloops of the receptor. Although the binding to the LRRs is well characterized the features of the peptide and receptor involved in the exoloop interaction are currently unknown. This study was designed to determine the key INSL3 determinants for RXFP2 activation. A chimeric peptide approach was first utilized to demonstrate that the A-chain is critical for receptor activation. Replacement of the INSL3 A-chain with that from the related peptides INSL5 and INSL6 resulted in complete loss of activity despite only minor changes in binding affinity. Subsequent replacement of specific A-chain residues with those from the INSL5 peptide highlighted that the N-terminus of the A-chain of INSL3 is critical for its activity. Remarkably, replacement of the entire N-terminus with four or five alanine residues resulted in peptides with near native activity suggesting that specific residues are not necessary for activity. Additionally removal of two amino acids at the C-terminus of the A-chain and mutation of Lys-8 in the B-chain also resulted in minor decreases in peptide activity. Therefore we have demonstrated that the activity of the INSL3 peptide is driven predominantly by residues 5–9 in the A-chain, with minor additional contributions from the two C-terminal A-chain residues and Lys-8 in the B-chain. Using this new knowledge, we were able to produce a truncated INSL3 peptide structure which retained native activity, despite having 14 fewer residues than the parent peptide.
Predicting pervious and impervious storm runoff from urban drainage basins

Abstract Rainfall and runoff depths were analysed for 47 storms recorded on three urban drainage basins in Canberra, Australia. Three runoff mechanisms have been identified: runoff generated on effective impervious surfaces in all storms; runoff from pervious areas of small storage capacity during both large and small storms; and runoff from pervious areas of large storage capacity for larger storms. The data indicate that pervious surface runoff is generated on only a small part of the total basin area.
Recognition of Frequency Modulated Whistle-Like Sounds by a Bottlenose Dolphin (Tursiops truncatus) and Humans with Transformations in Amplitude, Duration and Frequency

Bottlenose dolphins (Tursiops truncatus) use the frequency contour of whistles produced by conspecifics for individual recognition. Here we tested a bottlenose dolphin’s (Tursiops truncatus) ability to recognize frequency modulated whistle-like sounds using a three alternative matching-to-sample paradigm. The dolphin was first trained to select a specific object (object A) in response to a specific sound (sound A) for a total of three object-sound associations. The sounds were then transformed by amplitude, duration, or frequency transposition while still preserving the frequency contour of each sound. For comparison purposes, 30 human participants completed an identical task with the same sounds, objects, and training procedure. The dolphin’s ability to correctly match objects to sounds was robust to changes in amplitude with only a minor decrement in performance for short durations. The dolphin failed to recognize sounds that were frequency transposed by plus or minus ½ octaves. Human participants demonstrated robust recognition with all acoustic transformations. The results indicate that this dolphin’s acoustic recognition of whistle-like sounds was constrained by absolute pitch. Unlike human speech, which varies considerably in average frequency, signature whistles are relatively stable in frequency, which may have selected for a whistle recognition system invariant to frequency transposition.
From Rule-Breaking to ROM-Hacking: Theorizing the Computer Game-as-Commodity

This paper develops a theory of the game as a commodity form by looking at the unique practices of console hackers and videogame emulation communities. This theory argues for the necessity of understanding a game's system of rules in relation to the material conditions and constraints of the media within which it is constructed and distributed. After deriving the computer game-as-commodity from a combination of institutional and material restrictions and protections on the free-play of the execution of game rules, I provide an account of emulation and ROM-hacking communities as a cultural critique and playful resistance of such commodification within the rigid legal and technological infrastructures of autonomous, executable, and copyrighted machine code. Rather than asking whether videogame emulation is “right or wrong” in the abstract, I examine the legal, economic, and aesthetic implications of emulation practices, asking what the efforts of the emulation and ROM-hacking community have to contribute to the study of console videogames. Finally, I argue that analyzing and embracing the efforts of a variety of practices within the emulation and ROM-hacking communities is helpful and essential to both mapping past struggles and tracing future paradigms of the computer game's contradictory status as a commodity to be consumed and an algorithm to be uncovered.
Spontaneous Glutamate Release Is Independent of Calcium Influx and Tonically Activated by the Calcium-Sensing Receptor

Spontaneous release of glutamate is important for maintaining synaptic strength and controlling spike timing in the brain. Mechanisms regulating spontaneous exocytosis remain poorly understood. Extracellular calcium concentration ([Ca2+]o) regulates Ca2+ entry through voltage-activated calcium channels (VACCs) and consequently is a pivotal determinant of action potential-evoked vesicle fusion. Extracellular Ca2+ also enhances spontaneous release, but via unknown mechanisms. Here we report that external Ca2+ triggers spontaneous glutamate release more weakly than evoked release in mouse neocortical neurons. Blockade of VACCs has no effect on the spontaneous release rate or its dependence on [Ca2+]o. Intracellular [Ca2+] slowly increases in a minority of neurons following increases in [Ca2+]o. Furthermore, the enhancement of spontaneous release by extracellular calcium is insensitive to chelation of intracellular calcium by BAPTA. Activation of the calcium-sensing receptor (CaSR), a G-protein-coupled receptor present in nerve terminals, by several specific agonists increased spontaneous glutamate release. The frequency of spontaneous synaptic transmission was decreased in CaSR mutant neurons. The concentration–effect relationship for extracellular calcium regulation of spontaneous release was well described by a combination of CaSR-dependent and CaSR-independent mechanisms. Overall these results indicate that extracellular Ca2+ does not trigger spontaneous glutamate release by simply increasing calcium influx but stimulates CaSR and thereby promotes resting spontaneous glutamate release.
Chaotic Properties of Mappings on a Probability Space

Sensitive dependence on initial conditions is widely understood as being the central idea of chaos. We first give sufficient conditions (both topological and ergodic) on an endomorphism to ensure the sensitivity property. Then, a strong sensitivity concept is introduced. Sufficient conditions on a transformation implying strong sensitivity are given. We also provide bounds for the strong sensitivity constant.
Social influence of hubs in information cascade processes

Purpose The purpose of this paper is to explore how hubs’ social influence on social network decisions can cause the behavior of information cascades in a market. Design/methodology/approach The authors establish understanding of the fundamental mechanism of information cascades through a computational simulation approach. Findings Eigenvector centrality, betweenness centrality, and PageRank are statistically correlated with the occurrence of information cascades among agents; the hubs’ incorrect decisions in the early diffusion stage can significantly cause misled shift cascades; and the bridge role of hubs is more influential than their pivotal position role in the process of misled shift cascades. Originality/value This implication can be extendable in the field of marketing, sequential voting, and technology, or innovation adoption.
Diffusion‐weighted imaging of the fetal brain in vivo

A method of performing diffusion‐weighted imaging (DWI) and diffusion tensor imaging (DTI) of the fetal brain in utero is proposed. The major difficulty of performing diffusion imaging in utero is the presence of motion. By modifying conventional single‐shot spin‐echo echo‐planar DWI with a short repetition time sequence, a sequence that performs DWI and DTI within a breath‐hold of the mother (13 sec and 18 sec, respectively) was devised. T1 weighting caused by the use of short repetition times is compensated by interspersing diffusion imaging with additional b=0 image acquisitions. In utero fetal brain DWI and DTI were performed using this sequence. Quantitative analysis revealed minimal differences in the obtained apparent diffusion coefficient (ADC; directionally averaged ADC) values when using this sequence. The method can be readily implemented in a clinical setting and is especially useful when scanning mothers who cannot tolerate lengthier breath‐holds. Magn Reson Med, 2007. © 2007 Wiley‐Liss, Inc.