Miracle-dz/UniFAR-FTUs huggingface dataset:
Diagnosis of Advanced Skin Cancer Using Infrared Spectral Histopathology

Diagnosis of advanced skin cancer using Infrared spectral histopathology Cassio A. Lima1, Luciana Correa2, Hugh J. Byrne3, Denise M. Zezell1 1Instituto de Pesquisas Energeticas e Nucleares, University of Sao Paulo, Sao Paulo, 05508-000, Brazil 2Faculdade de Odontologia, University of Sao Paulo, Sao Paulo, 05508-000, Brazil 3FOCAS Research Institute, Dublin Institute of Technology, Dublin, Dublin 8, Ireland cassiolima@usp.br Abstract: In this study, we investigated the feasibility of Infrared Spectral Histopathology to diagnose invasive squamous cell carcinoma using hematoxylin and eosin stained tissue placed on conventional glass slides, as used in the pathologist workflow. OCIS codes: (170.0170) Medical optics and biotechnology; (170.6510) Spectroscopy, tissue diagnostics
Closely Related Influenza Viruses Induce Contrasting Respiratory Tract Immunopathology

The swine-origin H1N1 virus which emerged in 2009 resulted in the first influenza pandemic of the 21st century. Although the majority of infections were moderate, a significant proportion of infections were severe and characterized by acute respiratory distress syndrome and pulmonary edema. We compared two isolates from the 2009 H1N1 pandemic; A/California/07/09 (CA/07) and A/Netherlands/602/09 (NL/602) viruses that share greater than 99% sequence identity. Though genetically similar, these viruses exhibit contrasting pathological effects. Mice that were infected with 800 plaque forming unit (PFU) of CA/07 virus rapidly lost weight, which was concurrent with detection of high pulmonary concentrations of MCP-1, MIG, IP-10 and TIMP-1. Initially, severe bronchiolar epithelial necrosis and acute respiratory distress was observed, followed by marked bronchiolar epithelial hyperplasia. Mononuclear cell infiltration was initially localized to perivascular and peribronchiolar interstitium and then spread to adjacent alveoli. Infiltrating cells were phenotypically CD11bhi, F4/80lo. In contrast, when mice were infected with 800 PFU of NL/602 virus, minimal weight loss was observed, and concentrations of cytokines in the lung were significantly lower. Inflammation was primarily restricted to the bronchioles and perivascular interstitium with minimal spread to alveoli. Infiltrating cells include foamy macrophages and surface markers were characterized as CD11blo/-, F4/80hi. These two genetically similar viruses can be useful strains with which to investigate immune-regulatory determinants of pathogenesis of influenza virus.
Emergent imitative behavior on a robotic arm based on visuo-motor associative memories

Mimicry and deferred imitation have often been considered as separate kinds of imitation. In this paper, we present a simple architecture for robotic arm control which can be used for both. The model is based on some dynamical equations [1], which provide a motor control with exploring and converging capacities. A visuo-motor map [2] is used to associate positions of the end effector in the visual space with proprioceptive position of the robotic arm. It enables a fast learning of the visuo-motor associations without needing to embed a priori information. The controller can be used both for accurate control and interaction. It has been implemented on a minimal robotic setup showing some interesting emergent properties. The robot can reproduce simple gestures in mimicry situation and finalized actions in deferred imitation situation. Moreover, it can even show some “intention” recognition abilities. Finally, the experiment of deferred imitation which inherits from learning by demonstration, also provides a good basis for cooperative and interactive experiments.
Teaching and Learning Science as Argument

The concept of science as argument, and the view that engaging in scientiÞc argumentation should play a key role in science education, has become widely endorsed. The case is made here that this objective will be enhanced by broad understanding of the nature of argument skills and their directions and patterns of development. A line of research directed to this goal is described. C © 2010 Wiley Periodicals, Inc. Sci Ed 94:810824, 2010
An ultra low power 2.4GHz CMOS VCO

A low power voltage-controlled oscillator (VCO) for IEEE 802.11b direct conversion receiver is demonstrated. The VCO is fabricated by TSMC 0.25-/spl mu/m; based on the measurement results, the tuning range is 187 MHz (7.78%); phase noise is -73.62dBc/Hz@600 kHz offset and -82.44dBc/Hz@1MHz offset at 2.4GHz. The circuit draws only 53/spl mu/A current for the VCO core from a 1.5V supply.
Early psychotherapy process and cluster B and C personality pathology: similarities and differences in interactions with symptomatic and interpersonal distress

Abstract In a prior study (Kolden & Klein, 1996), the authors found that the relationships between global personality pathology and early psychotherapy change processes (as defined by the Generic Model of Psychotherapy) were moderated by the extent of the patient's acute symptomatic and interpersonal distress. In the current study, the authors reanalyzed the same data to examine similarities and differences between personality disorder Clusters B (dramatic, emotional, or erratic) and C (anxious or fearful) in therapy process. In general, we found that more distressed patients reported greater defensiveness. There were no significant interactions between symptomatic distress and personality pathology in the prediction of any of the process variables. However, interpersonal distress moderated relationships between Clusters B and C and some therapy processes. Patients high in Cluster B felt more open and involved in the session when they were less distressed by their interpersonal problems at the start of therapy. In contrast, openness and insight were impeded among patients high in Cluster C when they were less distressed interpersonally. Therapists generally used more direct interventions and exploration of past experiences when working with patients higher in Cluster C pathology. However, therapists used direct interventions more specifically when patients with more severe Cluster B pathology were also higher in interpersonal distress. The discussion considers implications for the facilitation of productive early therapy process in patients suffering from Cluster B or C personality pathology.
Endosperm origin, development, and function.

Endosperm has been studied from a variety of vantage points: evolution, role in seed development and germination, genetics, physiology, and biochemistry. This tissue represents a renewable, biodegradable source of materials; much effort has been directed to improve its use in feed and food making as well as its refinement to secondary products such as oils and plastics. Although there is a vast literature dealing with each of these topics, we still have a remarkably superficial understanding of most of them. There has been revitalized interest in understanding the endosperm in relation to seed-specific developmental processes. lnformation from these studies could provide a basis for developing more efficient approaches for plant improvement and use. Recent advances in molecular biology have created the possibility for detailed study of many of the genetic and molecular mechanisms involved in endosperm development. This research could conceivably lead to answers to many basic questions in developmental biology as well as to new tools that enhance practical uses of endosperm. In this review, it is not our intention to present a comprehensive overview of what is known about endosperm. Rather, we have chosen to summarize some of the research that has been done, making note of comprehensive reviews and pointing out important questions that remain open to scientific inquiry.
The power of interaction

Part 1 Preliminaries: basic definitions - notation and basics, Boolean formulas, arithmetic formulas and expressions computational models - deterministic computation, probabilistic computation, non-deterministic computation, alternating computation, interactive proof system, multiple prover interactive proof systems computation relative to an oracle complexity classes - deterministic complexity classes, probabilistic complexity classes, non-deterministic complexity clases, alternatiing complexity classes, interactive proof system complexity classes, non-uniform complexity classes, complexity classes relative to oracles counting clases completeness summary of our results. Part 2 The power of interaction with one prover: proof systems for #P - arithmetizing of #P, proof systems for #P proof systems for PSPaCE - arithmetizing of PSPACE, proof systems for PSPACE. Part 3 The power of interaction with two provers: another characterization of MIP arithmetization of NEXP computation multiple prover interactive proofs for NEXP - implementing the protocol with two provers, the power of the provers verification of multilinear functions - the test for multilinearity, the expansion Lemma, the self-improvement Lemma, the pasting Lemma, the tree colouring Lemma. Part 4 Interaction versus alternation: restricted alternating turing machines arithmetization of alternating computation interactive proof systems for ATISP (t,s) alternating turing machines for pIPTISP(t,s) a hierarchy for pIPTISP(t,s) interactive proof systems for deterministic computation. Part 5 Implications and open problems: bounded-round interactive proofs zero-knowledge program testing and verification - robustness, instance checking, self-testing and self-correcting programs, comparison with the Blum-Luby-Rubinfield model uniform vs. nonuniform complexity recent developments further research.
Effect of abutment's height and framework alloy on the load distribution of mandibular cantilevered implant-supported prosthesis.

OBJECTIVES In cantilevered implant-supported complete prosthesis, the abutments' different heights represent different lever arms to which the abutments are subjected resulting in deformation of the components, which in turn transmit the load to the adjacent bone. The purpose of this in vitro study was to quantitatively assess the deformation of abutments of different heights in mandibular cantilevered implant-supported complete prosthesis. MATERIAL AND METHODS A circular steel master cast with five perforations containing implant replicas (Theta 3.75 mm) was used. Two groups were formed according to the types of alloy of the framework (CoCr or PdAg). Three frameworks were made for each group to be tested with 4, 5.5 and 7 mm abutments. A 100 N load was applied at a point 15 mm distal to the center of the terminal implant. Readings of the deformations generated on the mesial and distal aspects of the abutments were obtained with the use of strain gauges. RESULTS Deformation caused by tension and compression was observed in all specimens with the terminal abutment taking most of the load. An increase in deformation was observed in the terminal abutment as the height was increased. The use of an alloy of higher elastic modulus (CoCr) also caused the abutment deformation to increase. CONCLUSION Abutment's height and framework alloy influence the deformation of abutments of mandibular cantilevered implant-supported prosthesis.
Robust video watermarking scheme in transform domains

Due to large amount of frames, similarity between frames and temporal attacks (frame dropping, frame averaging, frame swapping etc.), video watermarking process is more difficult than image watermarking. Current image watermarking methods are not adequate to solve these difficulties. We propose a novel uncompressed video watermarking system based on Hidden Markov Model (HMM) and Artificial Neural Network (ANN). The proposed watermarking scheme splits the video sequences into Group of Pictures (GOP) with HMM. Portions of the binary watermark will be embedded into each GOP with a selected transformation domain watermarking algorithm. For each GOP, ANN produces the optimal transformation algorithm. The embedding process is the standard additive algorithm in low and high frequencies in different transformation domains. This novel system increases the robustness against geometric and temporal attacks, and increases the quality of the watermarked video. Keywords—Watermarking, Hidden Markov Model, Neural Network, Frequency Domain, Group of Pictures.
Anti-apoptotic and growth-stimulatory functions of CK1 delta and epsilon in ductal adenocarcinoma of the pancreas are inhibited by IC261 in vitro and in vivo

Background: Pancreatic ductal adenocarcinomas (PDACs) are highly resistant to treatment due to changes in various signalling pathways. CK1 isoforms play important regulatory roles in these pathways. Aims: We analysed the expression levels of CK1 delta and epsilon (CK1δ/∊) in pancreatic tumour cells in order to validate the effects of CK1 inhibition by 3-[2,4,6-(trimethoxyphenyl)methylidenyl]-indolin-2-one (IC261) on their proliferation and sensitivity to anti-CD95 and gemcitabine. Methods: CK1δ/∊ expression levels were investigated by using western blotting and immunohistochemistry. Cell death was analysed by FACS analysis. Gene expression was assessed by real-time PCR and western blotting. The putative anti-tumoral effects of IC261 were tested in vivo in a subcutaneous mouse xenotransplantation model for pancreatic cancer. Results: We found that CK1δ/∊ are highly expressed in pancreatic tumour cell lines and in higher graded PDACs. Inhibition of CK1δ/∊ by IC261 reduced pancreatic tumour cell growth in vitro and in vivo. Moreover, IC261 decreased the expression levels of several anti-apoptotic proteins and sensitised cells to CD95-mediated apoptosis. However, IC261 did not enhance gemcitabine-mediated cell death either in vitro or in vivo. Conclusions: Targeting CK1 isoforms by IC261 influences both pancreatic tumour cell growth and apoptosis sensitivity in vitro and the growth of induced tumours in vivo, thus providing a promising new strategy for the treatment of pancreatic tumours.
Focal muscular hyperplasia of the trachea.

An example of what we have termed focal muscular hyperplasia of the trachea is described. This lesion has not been previously documented, to our knowledge, in the English-language literature. Originating in the membranous portion of the trachea, it was composed of hyperplastic smooth muscle bundles and was responsible for the production of mild respiratory symptoms. While its pathogenesis is uncertain, we favor the possibility that it represents a response to some unknown mechanical or inflammatory stimulus.
Trophic ulcers-Practical management guidelines

ABSTRACT The management of patients with trophic ulcers and their consequences is difficult not only because it is a recurrent and recalcitrant problem but also because the pathogenesis of the ulcer maybe different in each case. Methodically and systematically evaluating and ruling out concomitant pathologies helps to address each patient′s specific needs and hence bring down devastating complications like amputation. With incidence of diabetes being high in our country, and leprosy being endemic too the consequences of neuropathy and angiopathy are faced by most wound care specialists. This article presents a review of current English literature available on this subject. The search words were entered in PubMed central and appropriate abstracts reviewed. Relevant full text articles were retrieved and perused. Cross references from these articles were also reviewed. Based on these articles and the authors′ experiences algorithms for management have been presented to facilitate easier understanding. It is hoped that the information presented in this article will help in management of this recalcitrant problem.
An Update on the Sociomicrobiology of Quorum Sensing in Gram-Negative Biofilm Development

Bacteria are social creatures that are able to interact and coordinate behaviors with each other in a multitude of ways. The study of such group behaviors in microbes was coined “sociomicrobiology” in 2005. Two such group behaviors in bacteria are quorum sensing (QS) and biofilm formation. At a very basic level, QS is the ability to sense bacterial density via cell-to-cell signaling using self-produced signals called autoinducers, and biofilms are aggregates of cells that are attached to one another via a self-produced, extracellular matrix. Since cells in biofilm aggregates are in close proximity, biofilms represent an ecologically relevant environment for QS. While QS is known to affect biofilm formation in both Gram-negative and Gram-positive species, in this review, we will focus exclusively on Gram-negative bacteria, with an emphasis on Pseudomonas aeruginosa. We will begin by describing QS systems in P. aeruginosa and how they affect P. aeruginosa biofilm formation. We then expand our review to other Gram-negative bacteria and conclude with interesting questions with regard to the effect of biofilms on QS.
Critical Role for Interleukin-1β (IL-1β) during Chlamydia muridarum Genital Infection and Bacterial Replication-Independent Secretion of IL-1β in Mouse Macrophages

ABSTRACT Recent findings have implicated interleukin-1β (IL-1β) as an important mediator of the inflammatory response in the female genital tract during chlamydial infection. But how IL-1β is produced and its specific role in infection and pathology are unclear. Therefore, our goal was to determine the functional consequences and cellular sources of IL-1β expression during a chlamydial genital infection. In the present study, IL-1β−/− mice exhibited delayed chlamydial clearance and decreased frequency of hydrosalpinx compared to wild-type (WT) mice, implying an important role for IL-1β both in the clearance of infection and in the mediation of oviduct pathology. At the peak of IL-1β secretion in WT mice, the major producers of IL-1β in vivo are F4/80+ macrophages and GR-1+ neutrophils, but not CD45− epithelial cells. Although elicited mouse macrophages infected with Chlamydiamuridarum in vitro secrete minimal IL-1β, in vitro prestimulation of macrophages by Toll-like receptor (TLR) ligands such as lipopolysaccharide (LPS) purified from Escherichiacoli or C. trachomatis L2 prior to infection greatly enhanced secretion of IL-1β from these cells. By using LPS-primed macrophages as a model system, it was determined that IL-1β secretion was dependent on caspase-1, potassium efflux, and the activity of serine proteases. Significantly, chlamydia-induced IL-1β secretion in macrophages required bacterial viability but not growth. Our findings demonstrate that IL-1β secreted by macrophages and neutrophils has important effects in vivo during chlamydial infection. Additionally, prestimulation of macrophages by chlamydial TLR ligands may account for the elevated levels of pro-IL-1β mRNA observed in vivo in this cell type.
Paths to selection on life history loci in different natural environments across the native range of Arabidopsis thaliana

Selection on quantitative trait loci (QTL) may vary among natural environments due to differences in the genetic architecture of traits, environment‐specific allelic effects or changes in the direction and magnitude of selection on specific traits. To dissect the environmental differences in selection on life history QTL across climatic regions, we grew a panel of interconnected recombinant inbred lines (RILs) of Arabidopsis thaliana in four field sites across its native European range. For each environment, we mapped QTL for growth, reproductive timing and development. Several QTL were pleiotropic across environments, three colocalizing with known functional polymorphisms in flowering time genes (CRY2, FRI and MAF2‐5), but major QTL differed across field sites, showing conditional neutrality. We used structural equation models to trace selection paths from QTL to lifetime fitness in each environment. Only three QTL directly affected fruit number, measuring fitness. Most QTL had an indirect effect on fitness through their effect on bolting time or leaf length. Influence of life history traits on fitness differed dramatically across sites, resulting in different patterns of selection on reproductive timing and underlying QTL. In two oceanic field sites with high prereproductive mortality, QTL alleles contributing to early reproduction resulted in greater fruit production, conferring selective advantage, whereas alleles contributing to later reproduction resulted in larger size and higher fitness in a continental site. This demonstrates how environmental variation leads to change in both QTL effect sizes and direction of selection on traits, justifying the persistence of allelic polymorphism at life history QTL across the species range.
A silicon ultrasonic horn with a Bézier profile for surgical applications

A novel silicon ultrasonic horn with microprobes is developed for surgical applications. During insertion, penetration force of the microprobes driven by the ultrasonic horn can be reduced and tissue damage is minimized. The new horn has a profile of a Bézier curve. The dynamical characteristics of the horn are examined. Microscale prototypes are fabricated. Using the driving technique, the force reduction of microprobes during insertion will be investigated by experiments. Microprobes driven by the ultrasonic horns have potential applications in cardiac electrophysiology to provide information of wave propagation in hearts for understanding the mechanism of cardiac arrhythmia.
A Role for Health Communication in the Continuum of HIV Care, Treatment, and Prevention

Abstract:Health communication has played a pivotal role in HIV prevention efforts since the beginning of the epidemic. The recent paradigm of combination prevention, which integrates behavioral, biomedical, and structural interventions, offers new opportunities for employing health communication approaches across the entire continuum of care. We describe key areas where health communication can significantly enhance HIV treatment, care, and prevention, presenting evidence from interventions that include health communication components. These interventions rely primarily on interpersonal communication, especially individual and group counseling, both within and beyond clinical settings to enhance the uptake of and continued engagement in care. Many successful interventions mobilize a network of trained community supporters or accompagnateurs, who provide education, counseling, psychosocial support, treatment supervision, and other pragmatic assistance across the care continuum. Community treatment supporters reduce the burden on overworked medical providers, engage a wider segment of the community, and offer a more sustainable model for supporting people living with HIV. Additionally, mobile technologies are increasingly seen as promising avenues for ongoing cost-effective communication throughout the treatment cascade. A broader range of communication approaches, traditionally employed in HIV prevention efforts, that address community and sociopolitical levels through mass media, school- or workplace-based education, and entertainment modalities may be useful to interventions seeking to address the full care continuum. Future interventions would benefit from development of a framework that maps appropriate communication theories and approaches onto each step of the care continuum to evaluate the efficacy of communication components on treatment outcomes.