Miracle-dz/UniFAR-FTUs huggingface dataset:
Democracy and Deliberation: New Directions for Democratic Reform

This book proposes a new kind of democracy for the modern era, one that not only gives citizens more power but also allows them more opportunities to exercise this power thoughtfully. James S. Fishkin here suggests an innovative solution to the problem of inadequate deliberation, in particular within our presidential nomination system. His reform involves a well-publicized national caucus in which a representative sample of American citizens would interact directly with presidential contenders in order to reflect and vote on the issues and candidates. In adapting democracy to the large scale nation state, says Fishkin, Americans have previously had two choices. They could participate directly through primaries and referenda or they could depend on elite groups-such as party conventions and legislatures-to represent them. The first choice offers political equality but little chance for deliberation; the second offers the participants an opportunity to deliberate but provides less political equality for the electorate. The national caucus that Fishkin proposes-an example of what he calls a "deliberative opinion poll"-combines deliberation with political equality and reveals what the public would think if it had better conditions and information with which to explore and define the issues with the candidates. Arguing persuasively for the usefulness of deliberative opinion polls, Fishkin places them within the history of democratic theory and practice, exploring models of democracy ranging form ancient Athens and the debates of the American founders to contemporary transitions toward democracy in Eastern Europe.
RSC: a Reliable High I/O Performance Storage Cluster for Multimedia Applications

The increasing speed mismatch between storage device and processor has fueled a search for new storage structures and retrieval mechanisms. This paper describes the design and prototype implementation of RSC, a reliable high-performance clustered storage system for multimedia applications. To achieve high reliability, we adopt a novel hierarchy management method to ensure the metadata security and consistency. The adaptive data migration mechanism aims at keeping system load balance. With a dynamic membership management, RSC can be configured and maintained incrementally without interrupting its normal operation. Performance test results show that such a storage cluster provide good features and high performance through two groups of control experiments
Development and Evaluation of Thymol Microparticles Using Cellulose Derivatives as Controlled Release Dosage form

Thymol, an important and advantageous component of many essential oils, has been applied as an antimicrobial agent in animals. To increase the duration of action of this compound in ruminants, it was decided here to prepare a controlled release carrier for thymol. Hydroxy propyl methyl cellulose (HPMC) and ethyl cellulose (EC) were used as the matrix polymer here. Mixtures of thymol with eight different ratios of these polymers were then prepared using emulsion solvent evaporation method (F1 to F8). The prepared microparticles were evaluated for production yield, entrapment efficiency, drug content, particle size, drug release behavior, release kinetics (zero order, first order and Fickian matrix diffusion for spheres) and characterized by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Mean particle size of microparticles was 1.03 ± 0.02 mm. SEM study revealed that the microparticles were slightly irregular, rough and porous. The formulation with HPMC: EC ratio of 5:1 (F6) showed the highest drug loading (38.8%) and entrapment efficiency (61.2%). This formulation also showed optimum in-vitro drug release. The best fit of release kinetics was achieved with Fickian matrix diffusion for spheres (linear amount released vs t0.43). The FTIR spectroscopic and DSC studies show possible interaction between drug and polymers. In this study, thymol was successfully loaded in microparticles prepared from HPMC and EC. These microparticles can be used in further trials to evaluate the effect of slow release thymol on rumen fermentation parameters in ruminants.
How do we assess in Clinical Legal Education? A “reflection” about reflective learning

Institutional assessment must be distinguished from the assessment of learning. Traditionally, assessment is reduced to institutional assessment: that is, to give a mark depending on the achievement of knowledge instead of focusing in the student’s learning. However, I propose (to remember) that: 1) (Formative) assessment is part of learning; 2) Reflective learning (and reflective skills) is/are a part of assessment. This implies a process of continuous evaluation instead of summative evaluation, for example, through an exam or a similar procedure. So, I agree with the idea that assessment "is not a measurement problem but an instructional design problem. " To clarify what assessment is, we have to discuss several interlinked aspects (validity, reliability and fairness), which are connected to questions that must be answered: When is the assessment considered valid? How do we assess? What do we assess? Some ideas to answer these questions may include the need to provide space (s) and time (s) to reflect on the learning (as a way of learning and as a skill to be acquired), which in turn implies a multiplicity of assessments and/or reflection about learning. This should also include a variety of assessments: self-assessment, peer-assessment, team-assessment, and (external) assessment. And last, but not least: as it is said, reflection should be considered not only a skill but a part of learning. Reflection about learning is an exercise that promotes life-long learning (including that among future lawyers). A reflection about context and experience is the first step for future professional action. The benefits of experiencing autonomy and reflection are the same in a real or in realistic environments. But the experience of responsibility requires a real environment.
The availability and affordability of selected essential medicines for chronic diseases in six low- and middle-income countries.

OBJECTIVE To assess the availability and affordability of medicines used to treat cardiovascular disease, diabetes, chronic respiratory disease and glaucoma and to provide palliative cancer care in six low- and middle-income countries. METHODS A survey of the availability and price of 32 medicines was conducted in a representative sample of public and private medicine outlets in four geographically defined areas in Bangladesh, Brazil, Malawi, Nepal, Pakistan and Sri Lanka. We analysed the percentage of these medicines available, the median price versus the international reference price (expressed as the median price ratio) and affordability in terms of the number of days wages it would cost the lowest-paid government worker to purchase one month of treatment. FINDINGS In all countries<or=7.5% of these 32 medicines were available in the public sector, except in Brazil, where 30% were available, and Sri Lanka, where 28% were available. Median price ratios varied substantially, from 0.09 for losartan in Sri Lanka to 30.44 for aspirin in Brazil. In the private sector in Malawi and Sri Lanka, the cost of innovator products (the pharmaceutical product first given marketing authorization) was three times more than generic medicines. One month of combination treatment for coronary heart disease cost 18.4 days wages in Malawi, 6.1 days wages in Nepal, 5.4 in Pakistan and 5.1 in Brazil; in Bangladesh the cost was 1.6 days wages and in Sri Lanka it was 1.5. The cost of one month of combination treatment for asthma ranged from 1.3 days wages in Bangladesh to 9.2 days wages in Malawi. The cost of a one-month course of intermediate-acting insulin ranged from 2.8 days wages in Brazil to 19.6 in Malawi. CONCLUSION Context-specific policies are required to improve access to essential medicines. Generic products should be promoted by educating professionals and consumers, by implementing appropriate policies and incentives, and by introducing market competition and/or price regulation. Improving governance and management efficiency, and assessing local supply options, may improve availability. Prices could be reduced by improving purchasing efficiency, eliminating taxes and regulating mark-ups.
Prognostic value of PS2 and cathepsin D in 710 human primary breast tumors: multivariate analysis.

PURPOSE Evaluation of the prognostic value of cytosolic PS2 (pS2 protein) and cathepsin D in a large series of breast cancer patients by multivariate analysis taking into account steroid receptors and conventional prognostic factors. PATIENTS AND METHODS Prognostic factors were analyzed in 710 primary breast cancers (median follow-up, 4 years). PS2 and cathepsin D were measured by radiometric immunoassays. Estrogen receptor (ER) and progesterone receptor (PgR) status were assessed by radioligand binding assays and multiple-point Scatchard analysis. RESULTS The best cutoff point for PS2 to discriminate between positive (61% of the tumors) and negative was 2 ng/mg protein (univariate P value in 5-year relapse-free survival = .003). For cathepsin D, no sensible cutoff point could be chosen, since there was a continuous association between the level of cathepsin D and relapse rate (P = .001). In Cox multivariate analysis, relapse rate decreased with age of premenopausal/perimenopausal patients and with PS2 or steroid receptor positivity, and increased with the size of the tumor, the number of positive lymph nodes, and increasing levels of cathepsin D. In analysis for overall survival, age of both premenopausal/perimenopausal and postmenopausal patients, tumor size, the number of positive lymph nodes, ER/PgR, and PS2 were all independently associated with the rate of death. The level of cathepsin D was positively correlated with the rate of death, but this trend was not statistically significant. Separate Cox multivariate analyses for relapse-free survival in subgroups of patients as defined by nodal status showed that the contribution of PS2 and cathepsin D was the strongest in the node-negative subgroup. Node-negative patients with tumors containing PS2 values < or = 2 ng/mg protein and cathepsin D values more than 70 pmol/mg protein experienced a 4.5-fold increase in relapse rate as compared with those with PS2 levels greater than 2 ng/mg protein and cathepsin D levels < or = 30 pmol/mg protein. CONCLUSION PS2 and cathepsin D are independent prognostic factors in primary breast cancer and lymph node-negative patients.
Inshallah: Religious invocations in Arabic topic transition

Abstract The phrase inshallah ‘God willing’ is well known, even to non-Arabic speakers, as a mitigator of any statement regarding the future, or hopes for the future. Here we use the methods of conversation analysis (CA) to examine a less salient but nonetheless pervasive and compelling interactional usage: in topic-transition sequences. We use a corpus of Levantine (predominantly Syrian) Arabic talk-in-interaction to pay detailed attention to the sequential contexts of inshallah and its cognates across a number of exemplars. It emerges that these invocations are used to secure possible sequence and topic closure, and that they may engender reciprocal invocations. Topical talk following invocations or their responses is subsequently shown to be suspended by both parties; this provides for a move to a new topic by either party. (Arabic, religious expressions, conversation, conversation analysis, topic)*
Statistical Genetic Interval-Valued Fuzzy Systems with Prediction in Clinical Trials

In recent years, statistical tools and computational intelligence methods have played important roles in many areas. After statistically optimizing interval-valued fuzzy membership functions in the type-2 fuzzy logic system (FLS), we continue to apply genetic algorithms (GA) to optimize them. The proposed method is used to predict survival times for patients in clinical trials. The results show that the new GA-based method is more accurate than traditional type-1 and type-2 methods.
Searching for Causes of Poor Classification Performance in a Brain-Computer Interface Speller

The BCI Speller is a machine that flashes different letters on a screen and collects EEG data from the viewer, then analyzes the data to determine what letter the viewer was concentrating on. This allows a user to spell words without any body movements, which is useful for cases of heavy paralysis where speech is inhibited. The neurological event that this machine searches for is the P300, a pulse of electrical activity throughout the brain about 300 milliseconds after an unlikely event. However, the P300 has been noted as a varying event, and likewise the effectiveness of the Speller is highly variable: some tests will result in perfectly accurate spelling, while another test with the same user and conditions can be so inaccurate as to guess no letters correctly. The focus of this research was to search for a fundamental difference between highscoring and poor-scoring Speller tests. This could point towards a physical cause of inaccuracy, or could guide the way for a classification system that performs better in the case of a poor session. Two signal features were determined particularly likely to be the cause of classification error – variance in the latency time of the P300 and the occurrence of large-scale noise artifacts. It is difficult to mathematically define the quantity of either of these features; instead, classification methods that account for these features were designed and implemented on test sessions.
Intracellular pH Modulates Taste Receptor Cell Volume and the Phasic Part of the Chorda Tympani Response to Acids

The relationship between cell volume and the neural response to acidic stimuli was investigated by simultaneous measurements of intracellular pH (pHi) and cell volume in polarized fungiform taste receptor cells (TRCs) using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) in vitro and by rat chorda tympani (CT) nerve recordings in vivo. CT responses to HCl and CO2 were recorded in the presence of 1 M mannitol and specific probes for filamentous (F) actin (phalloidin) and monomeric (G) actin (cytochalasin B) under lingual voltage clamp. Acidic stimuli reversibly decrease TRC pHi and cell volume. In isolated TRCs F-actin and G-actin were labeled with rhodamine phalloidin and bovine pancreatic deoxyribonuclease-1 conjugated with Alexa Fluor 488, respectively. A decrease in pHi shifted the equilibrium from F-actin to G-actin. Treatment with phalloidin or cytochalasin B attenuated the magnitude of the pHi-induced decrease in TRC volume. The phasic part of the CT response to HCl or CO2 was significantly decreased by preshrinking TRCs with hypertonic mannitol and lingual application of 1.2 mM phalloidin or 20 μM cytochalasin B with no effect on the tonic part of the CT response. In TRCs first treated with cytochalasin B, the decrease in the magnitude of the phasic response to acidic stimuli was reversed by phalloidin treatment. The pHi-induced decrease in TRC volume induced a flufenamic acid–sensitive nonselective basolateral cation conductance. Channel activity was enhanced at positive lingual clamp voltages. Lingual application of flufenamic acid decreased the magnitude of the phasic part of the CT response to HCl and CO2. Flufenamic acid and hypertonic mannitol were additive in inhibiting the phasic response. We conclude that a decrease in pHi induces TRC shrinkage through its effect on the actin cytoskeleton and activates a flufenamic acid–sensitive basolateral cation conductance that is involved in eliciting the phasic part of the CT response to acidic stimuli.
Dissecting the Transcriptional Response to Elicitors in Vitis vinifera Cells

The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate, which, in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.
Modification of Ad5 Hexon Hypervariable Regions Circumvents Pre-Existing Ad5 Neutralizing Antibodies and Induces Protective Immune Responses

The development of an effective malaria vaccine is a high global health priority. Vaccine vectors based on adenovirus type 5 are capable of generating robust and protective T cell and antibody responses in animal models and are currently being evaluated in clinical trials for HIV and malaria. They appear to be more effective in terms of inducing antigen-specific immune responses as compared with non-Ad5 serotype vectors. However, the high prevalence of neutralizing antibodies to Ad5 in the human population, particularly in the developing world, has the potential to limit the effectiveness of Ad5-based vaccines. We have generated novel Ad5-based vectors that precisely replace the hexon hypervariable regions with those derived from Ad43, a subgroup D serotype with low prevalence of neutralizing antibody in humans. We have demonstrated that these hexon-modified adenovectors are not neutralized efficiently by Ad5 neutralizing antibodies in vitro using sera from mice, rabbits and human volunteers. We have also generated hexon-modified adenovectors that express a rodent malaria parasite antigen, PyCSP, and demonstrated that they are as immunogenic as an unmodified vector. Furthermore, in contrast to the unmodified vector, the hexon-modified adenovectors induced robust T cell responses in mice with high levels of Ad5 neutralizing antibody. We also show that the hexon-modified vector can be combined with unmodified Ad5 vector in prime-boost regimens to induce protective responses in mice. Our data establish that these hexon-modified vectors are highly immunogenic even in the presence of pre-existing anti-adenovirus antibodies. These hexon-modified adenovectors may have advantages in sub-Saharan Africa where there is a high prevalence of Ad5 neutralizing antibody in the population.
Gold nanoparticles enhance the radiation therapy of a murine squamous cell carcinoma

The purpose of this study is to test the hypothesis that gold nanoparticle (AuNP, nanogold)-enhanced radiation therapy (nanogold radiation therapy, NRT) is efficacious when treating the radiation resistant and highly aggressive mouse head and neck squamous cell carcinoma model, SCCVII, and to identify parameters influencing the efficacy of NRT. Subcutaneous (sc) SCCVII leg tumors in mice were irradiated with x-rays at the Brookhaven National Laboratory (BNL) National Synchrotron Light Source (NSLS) with and without prior intravenous (iv) administration of AuNPs. Variables studied included radiation dose, beam energy, temporal fractionation and hyperthermia. AuNP-mediated NRT was shown to be effective for the sc SCCVII model. AuNPs were more effective at 42 Gy than at 30 Gy (both at 68 keV median beam energy) compared to controls without gold. Similarly, at 157 keV median beam energy, 50.6 Gy NRT was more effective than 44 Gy NRT. At the same radiation dose (∼42 Gy), 68 keV was more effective than 157 keV. Hyperthermia and radiation therapy (RT) were synergistic and AuNPs enhanced this synergy, thereby further reducing TCD50 s (tumor control dose 50%) and increasing long-term survivals. It is concluded that gold nanoparticles enhance the radiation therapy of a radioresistant mouse squamous cell carcinoma. The data show that radiation dose, energy and hyperthermia influence efficacy and better define the potential utility of gold nanoparticles for cancer x-ray therapy.
Doing Farm Tourism: The Intertwining Practices of Gender and Work

Drawing on the perspective of doing gender, Berit Brandth and Marit S. Haugen explore how women and men do gender in farm tourist work. On the basis of five case studies of farms that have shifted from farm production to hosting tourists, the expectation is that the new occupation of tourism may create conditions for (un)doing gender at the interactional level and reshuffling power within the couple. The segmented work and unequal work statuses of men and women known from research on family farming seem to be less distinct in farm tourism as women are managers and men do cleaning, catering, and caring. However, the symbolic meaning of the indoor‐outdoor dichotomy plays a defining role. And even if women and men have changed their performances, gender and work are still interpreted and perceived according to the heterosexual matrix.
Octreotide Inhibits the Bilirubin Carriers Organic Anion Transporting Polypeptides 1B1 and 1B3 and the Multidrug Resistance-Associated Protein 2

The somatostatin analog octreotide can lead to hyperbilirubinemia without evidence of liver injury. Here we investigate whether octreotide inhibits the main sinusoidal/canalicular bilirubin carriers and whether it is a transport substrate. Octreotide showed the most potent inhibitory effect toward OATP1B1-mediated transport and weaker inhibition for OATP1B3- and MRP2-mediated transport. Octreotide had no effect on OATP2B1-mediated transport. Octreotide inhibited [3H]estradiol-17-β-glucuronide (E17βG) influx mediated by OATP1B1, 1B3, and multidrug resistance-associated protein 2 (MRP2) in a concentration-dependent manner, and the IC50 values were computed to be 23 μM (95% confidence interval [CI] 18-29), 68 μM (95% CI 50-91), and 116.6 μM (95% CI 74.5–182.4), respectively. The interaction between octreotide and OATP1B1 was further studied. Inhibition of [3H]E17βG OATP1B1-mediated transport was purely competitive with no changes in maximum transport capacity (Vmax) and a twofold Km increase when the influx kinetics of [3H]E17βG were measured in the presence of octreotide (8.8 ± 3.1 versus 4.4 ± 1.2 μM, P = 0.03). The inhibition constant (Ki) of octreotide for the transport of [3H]E17βG was calculated at 33.5 ± 5.5 μM. Uptake of radiolabeled octreotide by OATP1B1-CHO cells was higher than in wild-type CHO cells and nonlabeled octreotide at the extracellular compartment was able to trans-stimulate the OATP1B1-mediated efflux of intracellular [3H]E17βG, suggesting that octreotide is a substrate of OATP1B1. In summary, this study shows interaction of octreotide on the human hepatocellular bilirubin transporters OATP1B1, OATP1B3, and MRP2, notably OATP1B1. These findings are in line with the clinical observation that a fraction of patients under treatment with octreotide exhibit hyperbilirubinemia.