Miracle-dz/UniFAR-FTUs huggingface dataset:
Specific IgG activity against diarrheagenic bacteria in bovine immune milk and effect of pH on its antigen-binding activity upon heating

Bovine colostrum and milk antibodies of calving and lactating cows immunized with a multivalent vaccine consisting of whole cells of three different species of pathogenic bacteria including four strains of enterotoxigenic Escherischia coli, five strains of enteropathogenic Esch. coli, three strains of enteroinvasive Esch. coli, two strains of Samonella typhi, and one strain each of Shigellia dysenteriae, Sh. sonnei and Sh. flexneri were generated, respectively. A significantly elevated activity and titre of specific IgG from bovine immune colostrum were seen for only 5 days after calving of immunized cows, however, the levels of specific IgG could be obtained continuously from the milk of immunized lactating cows until the 11th week of the entire experiment period. Subsequently, we observed that the high specific IgG activity in immune milk was relatively stable under pH 5·0–7·0 at 37°C. Of importance, we identified that the specific IgG preserved its biological function for high antigen-binding activity at pH 5·5–6·5 for 30 min of heat treatment at 70°C and for 350 s at 72°C. Our findings suggest that the specific IgG from milk antibodies of immunized lactating cows may be used as an abundant source of hyper-immune products for prevention of multibacteria-induced diarrhea, however, the effect of pH on its antigen-binding activity upon heating should be carefully considered and designed.
White Matter Tauopathy With Globular Glial Inclusions: A Distinct Sporadic Frontotemporal Lobar Degeneration

Frontotemporal lobar degenerations are a group of disorders characterized by circumscribed degeneration of the frontal and temporal lobes and diverse histopathologic features. We report clinical, neuropathologic, ultrastructural, biochemical, and genetic data on 7 individuals with a 4-repeat tauopathy characterized by the presence of globular glial inclusions (GGIs) in brain white matter. Clinical manifestations were compatible with the behavioral variant of frontotemporal dementia and included motor neuron symptoms; there was prominent neuronal loss in the frontal and temporal cortex, subiculum, and amygdala. The surrounding white matter showed abundant GGIs composed of abnormal filaments present mostly in oligodendrocytes. The severity of white matter tau abnormalities correlated with a reduction in myelin and axons and with microglial activation. Western blotting of sarkosyl-insoluble tau demonstrated the presence of 2 major tau bands of 64 and 68 kd. No mutations in the microtubule-associated protein tau gene were detected in 2 affected individuals. We propose that 4-repeat tau-immunoreactive GGIs are the neuropathologic hallmark of a distinct sporadic tauopathy with variable clinical presentations that include frontotemporal dementia and occasionally upper motor neuron disease. This type of tauopathy with GGIs expands the group of neurodegenerativedisorders in which oligodendroglial pathology predominates, beyond the synucleinopathy multiple system atrophy disorders.
Osteoporosis screening and risk management

Osteoporosis is common among older adults and results in costly osteoporotic fractures. Screening for this metabolic bone disorder is warranted in most older adults and clinicians must be diligent in identifying persons at risk. The evaluation should include an assessment of risk factors for falls, a bone density test, and consideration of possible secondary causes of osteoporosis. Several medications are available to improve bone density and decrease fractures. Adequate calcium and vitamin D intake (and treatment of vitamin D deficiency) are paramount in the management of osteoporosis.
Some complexity questions related to distributive computing(Preliminary Report)

Let M = {0, 1, 2, . , m—1} , N = {0, 1, 2,. , n—1} , and f:M × N → {0, 1} a Boolean-valued function. We will be interested in the following problem and its related questions. Let i &egr; M, j &egr; N be integers known only to two persons P1 and P2, respectively. For P1 and P2 to determine cooperatively the value f(i, j), they send information to each other alternately, one bit at a time, according to some algorithm. The quantity of interest, which measures the information exchange necessary for computing f, is the minimum number of bits exchanged in any algorithm. For example, if f(i, j) = (i + j) mod 2. then 1 bit of information (conveying whether i is odd) sent from P1 to P2 will enable P2 to determine f(i, j), and this is clearly the best possible. The above problem is a variation of a model of Abelson [1] concerning information transfer in distributive computions.
Improving personalized services in mobile commerce by a novel multicriteria rating approach

With the rapid growth of wireless technologies and mobile devices, there is a great demand for personalized services in m-commerce. Collaborative filtering (CF) is one of successful techniques to produce personalized recommendations for users. This paper proposes a novel approach to improve CF algorithms, where the contextual information of a user and the multicriteria ratings of an item are considered besides the typical information on users and items. The multilinear singular value decomposition (MSVD) technique is utilized to explore both explicit relations and implicit relations among user, item and criterion. We implement the approach in an existing m-commerce platform, and encouraging experimental results demonstrate its effectiveness.
Controlled Model Exploration

We provide a detailed analysis of very weak fragments of modal logic. Our fragments lack connectives that introduce non-determinism and they feature restrictions on the modal operators, which may lead to substantial reductions in complexity. Our main result is a general game-based characterization of the expressive power of our fragments over the class of nite structures.
Discovering habits of effective online support group chatrooms

For users of online support groups, prior research has suggested that a positive social environment is a key enabler of coping. Typically, demonstrating such claims about social interaction would be approached through the lens of sentiment analysis. In this work, we argue instead for a multifaceted view of emotional state, which incorporates both a static view of emotion (sentiment) with a dynamic view based on the behaviors present in a text. We codify this dynamic view through data annotations marking information sharing, sentiment, and coping efficacy. Through machine learning analysis of these annotations, we demonstrate that while sentiment predicts a user's stress at the beginning of a chat, dynamic views of efficacy are stronger indicators of stress reduction.
Design and fabrication of a flip-chip-on-chip 3-D packaging structure with a through-silicon via for underfill dispensing

This paper presents a new package design for multichip modules. The developed package has a flip-chip-on-chip structure. Four chips [simulating dynamic random access memory (DRAM) chips for demonstration purpose] are assembled on a silicon chip carrier with eutectic solder joints. The I/Os of the four chips are fanned-in on the silicon chip carrier to form an area array with larger solder balls. A through-silicon via (TSV) hole is made at the center of the silicon chip carrier for optional underfill dispensing. The whole multichip module is mounted on the printed circuit board by the standard surface mount reflow process. After the board level assembly and X-ray inspection, the underfill process is applied to some selected specimens for comparative study purpose. The underfill material is dispensed through the center TSV hole on the silicon chip carrier to encapsulate the solder joints and the four smaller chips. Subsequently, scanning acoustic microscopy (SAM) is performed to inspect the quality of underfill. After the board-level assembly, all specimens are subject to the accelerated temperature cycling (ATC) test. During the ATC test, the electrical resistance of all specimens is monitored. The experimental results show that the packages without underfill encapsulation may fail in less than 100 thermal cycles while those with underfill can last for more than 1200 cycles. From the dye ink analysis and the cross-section inspection, it is identified that the packages without underfill have failure in the silicon chip carrier, instead of solder joints. The features and merits of the present package design are discussed in details in this paper.
Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site

Monoclonal antibodies were made against a truncated form of human laminin isolated from placenta. 12 antibodies were isolated and characterized. All antibodies stained basement membranes in placenta and immunoprecipitated laminin from media of cultured choriocarcinoma cells. Three antibodies, 3E5, 4C7, and 4E10, partially blocked the neurite-promoting activity of laminin. Addition of a second antibody, goat anti-mouse IgG, caused more complete blocking of the activity. Two of the blocking antibodies, 4C7 and 4E10, reacted with epitopes within the globular domain at the end of the long arm of laminin, and the third one, 3E5, reacted at the end of the rod-like portion of the long arm adjacent to the globular domain, as shown by electron microscopy after rotary shadowing. Five nonblocking antibodies used in the same test reacted with epitopes in other domains of the molecule. Blocking antibodies 3E5 and 4E10 could be used in immunoblotting and both antibodies reacted with the same polypeptides in pepsin fragments of human laminin, the predominant polypeptides being approximately 400 kD. When a crude extract of human amnion was used as a source of intact laminin, the 4E10 antibody detected a single polypeptide of approximately 400 kD. A nonblocking antibody, 2E8, which reacted at the center of the laminin cross, reacted predominantly with a 200-kD polypeptide in human laminin fragments and exclusively with a 200-kD polypeptide in amnion extract and in rat laminin. Our results with human laminin match the results by Edgar, D. , R. Timpl, and H. Thoenen, 1984, EMBO (Eur. Mol. Biol. Organ.) J., 3:1463-1468, in which the neurite-promoting activity of mouse laminin resides at the end of the long arm, which is also the site for heparin binding. However, since the active fragments of human laminin did not bind to heparin, the neurite-promoting site should be different from the heparin-binding site. Our results further suggest that the neurite-promoting site may be contained in or close to the 400-kD component of laminin.
mobileDOK: culture in your pocket

This paper describes the Digital Oulu Cultural Database, a web-based multimedia database containing information about the local culture of the city of Oulu in Finland. The main focus of the paper is on the use of the application on mobile devices. Features of the system include multi-device browsing interfaces, which enable the use of the system on both personal computers and mobile devices, and the transcoding of media to fit the named terminals. The mobile version of the application was empirically tested in a field trial, and additional data was collected by logging actual use.
Intraocular Pressure Monitoring Sensors

Continuous measurement of intraocular pressure is important in the detection and treatment of glaucoma. While a point check of intraocular pressure in a doctor's office using indirect measurements such as the tonometer is helpful, it is inadequate to track circadian variation. Circadian variation is an independent risk factor in addition to elevated pressure levels. This paper is aimed at providing an up-to-date review of various intraocular pressure sensing techniques and in vivo sensor design approaches. The basic operating principles of various implantable sensors are reviewed and categorized into groups to delineate their differences. A discussion is presented identifying the drawbacks of existing designs and key design questions are proposed for future progress.
Kullback leibler estimation of distribution parameters for SAR data

It is essential for SAR automatic target recognition (ATR) to estimate the parameters of statistical modes in SAR images. Most studies have relied on the conventional methods, such as moments technique and maximum likelihood estimation (MLE) method. In this paper, we apply a kullback leibler (KL) method to parameters estimation for SAR data. The ant colony algorithm (ACA) is extended to search for the optimal parameters. Experiment results show that the KL method performs better than MLE method in all cases tested.
Change Requirement Traceability based Impact Analysis Methodology to evaluate Object-Oriented Software

It is a well known fact that software maintenance plays a major role and finds importance in software development life cycle. As object-oriented programming has become the standard, it is very important to understand the problems of maintaining object-oriented software systems. This paper aims at evaluating object-oriented software system through change requirement traceability – based impact analysis methodology. The major issues have been related to change impact algorithms and inheritance of functionality.
Clinical Determinants of the Outcome of Manual Stretching in the Treatment of Congenital Muscular Torticollis in Infants: A Prospective Study of Eight Hundred and Twenty-one Cases

Background: The natural history of congenital muscular torticollis and the outcome of different treatment modalities have been poorly investigated, and the results of treatment have varied considerably. Methods: The main objective of this prospective study was to evaluate the outcomes of 821 consecutive patients with congenital muscular torticollis who were first seen when they were less than one year old, were treated with a standardized program of manual stretching, and were followed for a mean of 4.5 years. Before treatment, the patients were classified into one of three clinical groups: (1) palpable sternomastoid tumor, (2) muscular torticollis (thickening and tightness of the sternocleidomastoid muscle), and (3) postural torticollis (torticollis but no tightness or tumor). Results: Of the 821 patients, 452 (55%) had a sternomastoid tumor; 276 (34%), muscular torticollis; and ninety-three (11%), postural torticollis. Multivariate analysis of the outcomes showed that (1) the duration of treatment was significantly associated with the clinical group (p < 0.0001), a passive rotation deficit of the neck (p < 0.0001), involvement of the right side (p < 0.0001), difficulties with the birth (p < 0.009), and age at presentation (p < 0.0001); (2) the overall final assessment score was associated with the rotation deficit (p = 0.02), age at presentation (p = 0.014), and duration of treatment (p < 0.0001); and (3) subsequent surgical treatment was required by 8% (thirty-four) of the 452 patients in the sternomastoid tumor group compared with 3% (eight) of the 276 patients in the muscular torticollis group and 0% (none) of the ninety-three patients in the postural torticollis group. Conclusions: This large prospective study demonstrated that controlled manual stretching is safe and effective in the treatment of congenital muscular torticollis when a patient is seen before the age of one year. The most important factors that predict the outcome of manual stretching are the clinical group, the initial deficit in rotation of the neck, and the age of the patient at presentation. Surgical treatment is indicated when a patient has undergone at least six months of controlled manual stretching and has residual head tilt, deficits of passive rotation and lateral bending of the neck of >15°, a tight muscular band or tumor, and a poor outcome according to our special assessment chart.
Association Testing of Novel Type 2 Diabetes Risk Alleles in the JAZF1, CDC123/CAMK1D, TSPAN8, THADA, ADAMTS9, and NOTCH2 Loci With Insulin Release, Insulin Sensitivity, and Obesity in a Population-Based Sample of 4,516 Glucose-Tolerant Middle-Aged Danes

OBJECTIVE— We evaluated the impact on diabetes-related intermediary traits of common novel type 2 diabetes–associated variants in the JAZF1 (rs864745), CDC123/CAMK1D (rs12779790), TSPAN8 (rs7961581), THADA (rs7578597), ADAMTS9 (rs4607103), and NOTCH2 (rs10923931) loci, which were recently identified by meta-analysis of genome-wide association data. RESEARCH DESIGN AND METHODS— We genotyped the six variants in 4,516 middle-aged glucose-tolerant individuals of the population-based Inter99 cohort who were all characterized by an oral glucose tolerance test (OGTT). RESULTS— Homozygous carriers of the minor diabetes risk G-allele of the CDC123/CAMK1D rs12779790 showed an 18% decrease in insulinogenic index (95% CI 10–27%; P = 4 × 10−5), an 18% decrease in corrected insulin response (CIR) (8.1–29%; P = 4 × 10−4), and a 13% decrease in the ratio of area under the serum-insulin and plasma-glucose curves during an OGTT (AUC-insulin/AUC-glucose) (5.8–20%; P = 4 × 10−4). Carriers of the diabetes-associated T-allele of JAZF1 rs864745 had an allele-dependent 3% decrease in BIGTT-AIR (0.9–4.3%; P = 0.003). Furthermore, the diabetes-associated C-allele of TSPAN8 rs7961581 associated with decreased levels of CIR (4.5% [0.5–8.4]; P = 0.03), of AUC-insulin/AUC-glucose ratio (3.9% [1.2–6.7]; P = 0.005), and of the insulinogenic index (5.2% [1.9–8.6]; P = 0.002). No association with traits of insulin release or insulin action was observed for the THADA, ADAMTS9, or NOTCH2 variants. CONCLUSIONS— If replicated, our data suggest that type 2 diabetes at-risk alleles in the JAZF1, CDC123/CAMK1D, and TSPAN8 loci associate with various OGTT-based surrogate measures of insulin release, emphasizing the contribution of abnormal pancreatic β-cell function in the pathogenesis of type 2 diabetes.
Learned self-regulation of the lesioned brain with epidural electrocorticography

Introduction: Different techniques for neurofeedback of voluntary brain activations are currently being explored for clinical application in brain disorders. One of the most frequently used approaches is the self-regulation of oscillatory signals recorded with electroencephalography (EEG). Many patients are, however, unable to achieve sufficient voluntary control of brain activity. This could be due to the specific anatomical and physiological changes of the patient’s brain after the lesion, as well as to methodological issues related to the technique chosen for recording brain signals. Methods: A patient with an extended ischemic lesion of the cortex did not gain volitional control of sensorimotor oscillations when using a standard EEG-based approach. We provided him with neurofeedback of his brain activity from the epidural space by electrocorticography (ECoG). Results: Ipsilesional epidural recordings of field potentials facilitated self-regulation of brain oscillations in an online closed-loop paradigm and allowed reliable neurofeedback training for a period of 4 weeks. Conclusion: Epidural implants may decode and train brain activity even when the cortical physiology is distorted following severe brain injury. Such practice would allow for reinforcement learning of preserved neural networks and may well provide restorative tools for those patients who are severely afflicted.
Preserving consistency of runtime monitors across protocol changes

Protocols governing communication among the components of a complex system are frequently changed during the design process. To enable faster verification turnaround time, it is important that the existing verification infrastructure continues to be consistent with the changed protocol. In this paper, an approach to identify the effects of protocol changes on runtime monitors is proposed. Runtime monitors are commonly used to observe and verify the dynamic protocol behaviors. Protocols as well as the monitors are modeled using communicating finite state machines. Addition/deletion/replacement of transitions in one or more protocol components may result in similar changes to the monitor transitions. A notion of consistency of a monitor relative to a protocol is introduced. Conditions under which a protocol change necessitates a change to the monitor to preserve relative consistency are identified. Automatic procedures to synthesize new monitors that are guaranteed to be consistent with the changed protocol are described.
Chronic treatment with Δ‐9‐tetrahydrocannabinol alters the structure of neurons in the nucleus accumbens shell and medial prefrontal cortex of rats

The potential of repeated exposure to Δ9‐tetrahydrocannabinol (Δ9‐THC) to produce long‐lasting changes in synaptic connections in a manner similar to other drugs of abuse was evaluated in Sprague‐Dawley rats. For 12 days, rats received two i.p. injections per day (8 h apart) of vehicle, a low dose of Δ9‐THC (0.5 mg/kg), or escalating doses of Δ9‐THC (0.5–4.0 mg/kg). Thirty days later, they were evaluated for sensitized locomotor activity (during the night cycle) for 60 min on each of three trials. Using a within‐groups design, rats were tested following an injection of vehicle, 0.5 mg/kg Δ9‐THC or 2.0 mg/kg Δ9‐THC. The rats showed no evidence of sensitized locomotor activity in any group. Twenty‐four hours after the final sensitization test, their brains were removed and then processed for Golgi‐Cox staining. Prior exposure to Δ9‐THC (both the low dose and the escalating doses) increased the length of the dendrites as well as the number of dendritic branches in the shell of the nucleus accumbens and in the medial prefrontal cortex, but not in the hippocampus, striatum, orbital frontal cortex, parietal cortex, or occipital cortex. These results are similar to those evident in brains of rats sensitized to amphetamine, and support previous findings that cannabinoids promote DA activity in the mesolimbic DA system. Synapse 60:429–436, 2006. © 2006 Wiley‐Liss, Inc.
Inhibitory Control at a Synaptic Relay

The mammalian medial nucleus of the trapezoid body (MNTB) harbors one of the most powerful terminals in the CNS, the calyx of Held. The mechanisms known to regulate this synaptic relay are relatively ineffective. Here, we report the presence of a remarkably robust and fast-acting glycinergic inhibitory system capable of suppressing calyceal transmission. Evoked glycinergic IPSCs were relatively small in 2-week-old rats, an age by which calyceal maturation has reportedly neared completion. However, by postnatal day 25 (P25), glycinergic transmission had undergone a vigorous transformation, resulting in peak synaptic conductances as high as 280 nS. These are comparable with glutamatergic conductances activated by calyceal inputs. Decay kinetics for IPSCs were severalfold faster than for glycinergic synaptic events reported previously. At physiological temperatures in P25 rats, IPSCs decayed in ∼1 msec and could be elicited at frequencies up to 500 Hz. Moreover, EPSPs triggered by glutamatergic signals derived from the calyx or simulated by conductance clamp were suppressed when preceded by simulated glycinergic IPSPs. The matching of excitatory transmission in the calyx of Held by a powerful, precision inhibitory system suggests that the relay function of the MNTB may be rapidly modified during sound localization.