Miracle-dz/UniFAR-FTUs huggingface dataset:
Apoptosis and proliferation of endothelial cells in early atherosclerotic lesions: possible role of oxidised LDL.

BACKGROUND AND AIM Evidence for apoptosis has been found in advanced atherosclerotic lesions, but the factors triggering it are poorly understood. Oxidised low-density lipoproteins (LDLs) are cytotoxic to a variety of cells and induce the apoptosis of smooth muscle cells (SMC), fibroblast, macrophages and endothelial cells in vitro. The aim of this study was to investigate apoptotic cell death in the early phases of aortic atherosclerosis in rabbits, and whether oxidised LDLs colocalize ex vivo with apoptotic cells in atherosclerotic lesions in cholesterol-fed rabbits. METHODS AND RESULTS Male albino New Zealand rabbits were fed a standard diet or a diet containing 1.2% cholesterol for 60 days. The aortic arch of each animal was sectioned and stained with antibodies against SMC, endothelial cells, macrophages and oxidised LDLs or for proteins involved in apoptotic pathways such as Fas, Bax, Bcl2, and caspase 3. The nuclei in adjacent sections were stained with Hoechst 33258, TUNEL and for the proliferating cell nuclear antigen (PCNA). Early atherosclerotic lesions were characterised by intimal thickening and the presence of SMC and macrophages. The percentage of apoptotic cells, calculated as the ratio of TUNEL-positive nuclei to total nuclei was 32.6 +/- 3.73% in the lesions and 55.9 +/- 2.36% in the endothelium. As it has been reported that nuclei undergoing active gene transcription can be TUNEL positive, we evaluated the percentage of PCNA-positive cells, which proved to be 45.2 +/- 4.68% along the endothelium and 22.3 +/- 2.7% in the intima. The true percentage of apoptotic cells was therefore about 10% in both cases. Fas, Bax and caspase3 signals were mainly located in the endothelium and SMC proximal to the lumen, whereas Bcl2 colocalized with macrophages and SMC deeper in the lesions. Abundant oxidised LDL epitopes were detected in areas of lipid accumulation and along the endothelium, mainly in the areas in which TUNEL and PCNA-positive cells were localised. CONCLUSIONS Our findings may be taken as ex vivo indications of an apoptotic and proliferating role of oxidised LDLs as previously shown in vitro, and may at least partially account for the endothelial dysfunction that can be rapidly induced by hypercholesterolemia.
Physicochemical study of the protein–liposome interactions: influence of liposome composition and concentration on protein binding

Abstract The aim of the present study is to investigate the interactions between liposomes and proteins and to evaluate the role of liposomal lipid composition and concentration in the formation of protein corona. Liposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or hydrogenated soybean phosphatidylcholine (HSPC) with 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-3000] (DPPE-PEG 3000), cholesterol (CH) or mixtures of these lipids, were prepared at different concentrations by the thin-film hydration method. After liposomes were dispersed in HPLC-grade water and foetal bovine serum (FBS), their physicochemical characteristics, such as size, size distribution, and ζ-potential, were determined using dynamic and electrophoretic light scattering. Aggregation of DPPC, HSPC, DPPC:CH (9:1 molar ratio), and HSPC:CH (9:1 molar ratio) in FBS was observed. On the contrary, liposomes incorporating DPPG lipids and CH both in a molar ratio of 11% were found to be stable over time, while their size did not alter dramatically in biological medium. Liposomes containing CH and PEGylated lipids retain their size in the presence of serum as well as their physical stability. In addition, our results indicate that the protein binding depends on the presence of polyethylene glycol (PEG), CH, concentration and surface charge. In this paper, we introduce a new parameter, fraction of stealthiness (Fs), for investigating the extent of protein binding to liposomes. This parameter depends on the changes in size of liposomes after serum incubation, while liposomes have stealth properties when Fs is close to 1. Thus, we conclude that lipid composition and concentration affect the adsorption of proteins and the liposomal stabilization.
Too Important to Ignore: Siblings of Children with Special Needs

Over the last decade there has been an increasing emphasis in Australia on the prevention of mental health problems. It has been accepted that the early years will have a profound effect on a child’s longer term health and development and that preventive approaches need to focus on these years. Governments have also started to see the economic benefits of investing in the early years. For example, it has been estimated that the cost of each case of depression is approximately $10,000 per annum (Sobocki, Jonsson, Angst & Rehnberg, 2006). If we can intervene early and reduce the incidence of such cases, the savings at a population level can be huge.
Co-evolution as Tool for Diversifying Flavor and Aroma Profiles of Wines

The products of microbial metabolism form an integral part of human industry and have been shaped by evolutionary processes, accidentally and deliberately, for thousands of years. In the production of wine, a great many flavor and aroma compounds are produced by yeast species and are the targets of research for commercial breeding programs. Here we demonstrate how co-evolution with multiple species can generate novel interactions through serial co-culture in grape juice. We find that after ~65 generations, co-evolved strains and strains evolved independently show significantly different growth aspects and exhibit significantly different metabolite profiles. We show significant impact of co-evolution of Candida glabrata and Pichia kudriavzevii on the production of metabolites that affect the flavor and aroma of experimental wines. While co-evolved strains do exhibit novel interactions that affect the reproductive success of interacting species, we found no evidence of cross-feeding behavior. Our findings yield promising avenues for developing commercial yeast strains by using co-evolution to diversify the metabolic output of target species without relying on genetic modification or breeding technologies. Such approaches open up exciting new possibilities for harnessing microbial co-evolution in areas of agriculture and food related research generally.
1alpha, 25-dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital.

AIM To investigate the chemopreventive effects of 1alpha, 25-dihydroxyvitamin D(3) in diethylnitrosamine induced, phenobarbital promoted rat hepatocarcinogenesis. METHODS The rats were randomly divided into 6 different groups (A-F). Groups A, C and E rats received a single intraperitoneal (i.p.) injection of diethylnitrosamine (DEN) at a dose of 200 mg/kg body mass in 9 g/L NaCl solution at 4 wk of age, while group B served as normal vehicle control received normal saline once. After a brief recovery of 2 wk, all the DEN treated rats were given phenobarbital (PB) at 0.5 g/L daily in the basal diet till wk 20. Group A was DEN control. Treatment of 1alpha, 25-(OH)2D3 in group C was started 4 wk prior to DEN injection and continued thereafter till wk 20 at a dose of 0.3 microg/100 microL propylene glycol per one single dose (os) twice a week. Group E received the treatment of 1alpha, 25-(OH)2D3 at the same dose mentioned as above for 15 wk. The rats in group D and F received 1alpha, 25-(OH)2D3 alone as in group C without DEN injection. RESULTS The comet assay showed statistically higher mean values for length to width ratios (L: W) of DNA mass and tailed cells (P<0.01; P<0.01 respectively) in DEN treated rats as compared to their normal controls. Continuous supplementation of 1alpha, 25-dihydroxyvitaminD2 showed a significant (P<0.01) decrease in L:W ratio of DNA mass tailed cells. Furthermore, 1alpha, 25-(OH)2D3 supplementations elevated the super oxide dismutase (SOD) activity, hepatic malondialdehyde (MDA) level, reduced glutathione (GSH) and glutathione S-transferase (GST) activity (P<0.01, P<0.05, P<0.05 and P<0.05 respectively). As an endpoint marker histological changes were observed to establish the chemopreventive effects of 1alpha, 25-dihydroxyvitaminD2. CONCLUSION Supplementations of 1alpha, 25-(OH)2D3 has a marked protection against hepatic nodulogenesis, antioxidant enzymes and DNA damages in DEN induced rat hepatocarcinogenesis promoted by phenobarbital.
Depression and risk of nursing home admission among older adults in home care in Europe: results from the Aged in Home Care (AdHOC) study.

OBJECTIVE Depression is a frequent condition observed among nursing home residents. However, so far, limited data are available on the impact of depression on nursing home admission. The aim of the present study was to assess the effect of depression on the risk of nursing home admission in a group of older adults receiving home care in Europe. METHOD We conducted a longitudinal analysis using data from the Aged in Home Care (AdHOC) database, which contains information on older adults receiving home care services in 11 European countries. Subjects had been admitted to the home care programs between 2001 and 2003. Depression was diagnosed as a score >or=3 on the Minimum Data Set (MDS) Depression Rating Scale. Information on nursing home admission was collected semiannually for 1 year by trained research personnel. RESULTS The mean age of 2718 older adults entering the study was 82.4 (SD = 7.3) years, 2047 (75.3%) were women, and 331 (12.2%) were depressed. Overall, 49/331 depressed participants (14.8%) and 252/2387 nondepressed participants (10.6%) were admitted to a nursing home (p = .02). After adjusting for potential confounders, the risk of nursing home admission was significantly higher for depressed participants (hazard ratio = 1.43, 95% CI = 1.02 to 2.02). The risk of nursing home admission progressively and significantly increased as MDS Depression Rating Scale score increased (signifying more severe depression) (p = .001 for linear trend). CONCLUSIONS In older adults receiving home care in Europe, depression is associated with an increased risk for nursing home admission. This association increases with severity of depression.
An appraisal of the trustworthiness of practice guidelines for depression and anxiety in children and youth

Little is known about the trustworthiness of clinical practice guidelines (PGs) relevant to child and youth depression or anxiety. To address this gap, we used systematic review methods to identify all available relevant PGs, quality appraise them, and make recommendations regarding which PGs are trustworthy and should be used by clinicians.
Fundamental Limits of Coded Linear Transform

In large scale distributed linear transform problems, coded computation plays an important role to effectively deal with "stragglers" (distributed computations that may get delayed due to few slow or faulty processors). We propose a coded computation strategy, referred to as diagonal code, that achieves the optimum recovery threshold and the optimum computation load. This is the first code that simultaneously achieves two-fold optimality in coded distributed linear transforms. Furthermore, by leveraging the idea from random proposal graph theory, we design two random codes that can guarantee optimum recovery threshold with high probability but with much less computation load. These codes provide order-wise improvement over the state-of-the-art. Moreover, the experimental results show significant improvement compared to both uncoded and existing coding schemes.
NF‐Y‐dependent regulation of glutamate receptor 4 expression and cell survival in cells of the oligodendrocyte lineage

Glutamate receptor subunit 4 (GluA4) is highly expressed by neural cells sensitive to excitotoxicity, and is the predominant subunit expressed by oligodendrocyte precursor cells (OPC) during a key period of vulnerability to hypoxic‐ischemic injury. Therefore, transcriptional networks downstream of excitotoxic GluA4 activation represent a promising area for therapeutic intervention. In this work, we identify the CCAAT binding transcription factor NF‐Yb as a novel transcriptional regulator of Gria4 (GluA4 gene), and a controller of excitotoxic death in the oligodendroglial lineage. We describe a novel regulatory region within Gria4 containing CCAAT sequences whose binding by NF‐Yb is regulated by excitotoxicity. Excitotoxicity‐induced alterations in NF‐Yb binding are associated with changes in Gria4 transcription, while knockdown of NF‐Yb alters the transcription of reporter constructs containing this regulatory region. Data from immortalized and primary OPC reveal that RNAi and pharmacological disruption of NF‐Yb alter Gria4 transcription, with the latter inducing apoptosis and influencing a set of apoptotic genes similarly regulated during excitotoxicity. These data provide the first definition of a trans‐acting mechanism regulating Gria4, and identify the NF‐Y network as a potential source of pharmacological targets for promoting OPC survival.
Bridging the digital divide: young people's perspectives on taking action

Marginalised young people really do care about political and social issues, but that their day-to-day experiences often make it difficult for them to take action to create change in their communities, according to the findings of this report. "Taking action" means something quite different to marginalised young people, compared with traditional definitions of social and political participation. The research found that some of the barriers to participating in social and political action included not knowing how to take action, the attitudes of others and their own personal circumstances and characteristics. For those most marginalised, issues such as safety, personal security and health were paramount. The research involved focus groups with young people, as well as in-depth interviews with service providers, across Victoria. It explored how young people from a diverse range of backgrounds feel about participating in political and social action. This report is the second in a series of four that explores the use of Information and Communication Technologies (ICT) and its role in improving the mental health of young people who experience or who are at risk of experiencing social, cultural or economic marginalisation.
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples.

OBJECTIVE To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. METHODS A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. RESULTS The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). CONCLUSIONS It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases.
Comparison of seven business model innovation tools for IoT ecosystems

This paper presents seven different business model innovation tools and how they compare when applied to business model innovation using the internet of thing ecosystem. The work leading up to this publication encompasses research that reviewed existing literature on business innovation tools and establishing internet of things ecosystem characteristic that need to be exposed when working with business model innovation. The conclusion is that the more mature and easy-to-use tools fall short on taking into consideration important internet of things characteristics, while the less mature specialized tools are more inline by exposing these characteristics but falling short on maturity and usability. The paper clearly identifies this gap.
Rheological properties of rice–blackgram batter while replacing white rice with brown rice

Rice–blackgram batter is a raw material for many traditional convenience foods in Asia. Reformulation of traditional convenience food by replacing white rice with whole rice (brown rice) is a novel method to reduce the consumption of refined grain and increase the intake of whole grain in our diet. In this study, rheological properties of rice–blackgram batter was investigated while replacing white rice with brown rice at five levels (T1 – 0% replacement (control), T2 – 25% replacement, T3 – 50% replacement, T4 – 75% replacement, and T5 – 100% replacement). The shear stress versus shear rate plot indicates that the rice–blackgram batter exhibited non-Newtonian fluid behavior (shear thinning property) even after 100% replacement of white rice with brown rice. The rheological characteristics of rice–blackgram batters fitted reasonably well in Cassan (r2 = 0.8521–0.9856) and power law (r2 = 0.8042–0.9823) models. Brown rice replacement at all levels did not affect the flow behavior index, yield stress, consistency coefficient, and apparent viscosity of batter at 25 ℃. However, at higher temperature, the viscosity was greater for T4 and T5 (no difference between them) than T1, T2, and T3 (no difference between them) batters. Further research is required to determine the sensory attributes and acceptability of the cooked products with brown rice-blended batter.
Subcutaneous ofatumumab in patients with relapsing-remitting multiple sclerosis

Objective To assess dose-response effects of the anti-CD20 monoclonal antibody ofatumumab on efficacy and safety outcomes in a phase 2b double-blind study of relapsing forms of multiple sclerosis (RMS). Methods Patients (n = 232) were randomized to ofatumumab 3, 30, or 60 mg every 12 weeks, ofatumumab 60 mg every 4 weeks, or placebo for a 24-week treatment period, with a primary endpoint of cumulative number of new gadolinium-enhancing lesions (per brain MRI) at week 12. Relapses and safety/tolerability were assessed, and CD19+ peripheral blood B-lymphocyte counts measured. Safety monitoring continued weeks 24 to 48 with subsequent individualized follow-up evaluating B-cell repletion. Results The cumulative number of new lesions was reduced by 65% for all ofatumumab dose groups vs placebo (p < 0.001). Post hoc analysis (excluding weeks 1–4) estimated a ≥90% lesion reduction vs placebo (week 12) for all cumulative ofatumumab doses ≥30 mg/12 wk. Dose-dependent CD19 B-cell depletion was observed. Notably, complete depletion was not necessary for a robust treatment effect. The most common adverse event was injection-related reactions (52% ofatumumab, 15% placebo), mild to moderate severity in 97%, most commonly associated with the first dose and diminishing on subsequent dosing. Conclusion Imaging showed that all subcutaneous ofatumumab doses demonstrated efficacy (most robust: cumulative doses ≥30 mg/12 wk), with a safety profile consistent with existing ofatumumab data. This treatment effect also occurred with dosage regimens that only partially depleted circulating B cells. Classification of evidence This study provides Class I evidence that for patients with RMS, ofatumumab decreases the number of new MRI gadolinium-enhancing lesions 12 weeks after treatment initiation.
Moral Distress (MD) and burnout in mental health nurses: a multicenter survey

Introduction: Moral Distress (MD) is a common experience among nurses and if it is not recognised and treated, it may lead to serious consequences on nurses’ health and quality of care. MD has been studied in several healthcare contexts. However, there are only few studies on MD in psychiatry and in Italy there are no data in this field. Objective: To assess the presence of MD among mental health nurses in Italy and verify whether there is a relationship between MD and burnout. Methods: We conducted a multicentre survey among nurses of Mental Health services of four hospitals in Milan. The MD Scale for Psychiatric NursesItalianrevised (MDS-PItarev) and the Maslach Burnout Inventory (MBI) were used for data collection. Factor analysis and calculation of content validity index (CVI) and Cronbach’s alpha were performed on the Italian version of the MDS-PIta. Three items of the old version of the scale were removed, because judged not relevant in the Italian context. The revised scale maintained excellent CVI (0.89) and Cronbach’s alpha (0.93). Results: Of 285 questionnaires distributed, 228 (80%) were returned. The median of MD was 2 (scale range 0-6); MD is correlated with two burnout dimensions: emotional exhaustion (rho(ρ)=0.28, p<0,001) and depersonalization (ρ=0.20, p<0,001). Conclusions: This is the first study about MD among psychiatric nurses in Italy. We found a medium-low level of MD among nurses who participated in the survey. We also found a modest but significant correlation between MD and two dimensions of MBI. Further studies are needed to confirm these results.
CIDADES INTELIGENTES E EMPREENDEDORAS: UM ESTUDO COMPARATIVO ENTRE RANKINGS

Com o crescimento populacional nas cidades se fazem necessarios estudos sobre os impactos no suprimento das necessidades basicas. Para suprir as demandas, e preciso que as cidades se tornem mais inteligentes e aliem tecnologia e desenvolvimento urbano. Alem disso, uma das razoes para as pessoas melhorarem a qualidade de vida nas cidades e o empreendedorismo. Baseando-se nisso, sao utilizados os conceitos de cidades inteligentes e cidades empreendedoras que buscam analisar as cidades sob diferentes aspectos. Como forma de mensuracao, os rankings aparecem sendo capazes de agregar vantagens para as cidades. Contudo, estes podem nao refletir a realidade e as necessidades das mesmas. Assim, este estudo, tem como objetivo realizar um comparativo de indicadores dos rankings de cidades inteligentes (Connected Smart Cities) e cidades empreendedoras (Endeavor) do ano de 2016 de forma a analisar a singularidade e a necessidade a que este ranking se propoe a suprir. Para isso, foi realizada uma pesquisa exploratoria, descritiva e qualitativa acerca dos rankings, em que se conduziu em quatro fases: selecao, identificacao de dimensoes, identificacao de indicadores e um comparativo entre eles. Como resultado, foi possivel perceber que os rankings possuem semelhancas em aspectos como infraestrutura, porem com enfoques diferentes, de modo a suprir a necessidade a que cada qual se propoe. Portanto, sao necessarios rankings mais aprofundados, baseados em conhecimento cientifico, de forma a indicar a realidade de cada tipologia de cidade.