Miracle-dz/UniFAR-FTUs huggingface dataset:
Exogenous kisspeptin (kp-10) resumes cyclicity in postpartum anestrus mithun cows

Present study was conducted to assess the effect of exogenous kisspeptin administration on estrus induction, Kiss1gene expression, endocrinological profiles and follicular development in post partum (PP) anestrus mithun cows. Animals who failed to resume cyclicity after day 90â€“100 of parturition were selected. Experimental cows were examined thoroughly through per-rectal as well as ultrasonograpic examination to confirm the anestrous status. PP anestrous healthy mithun cows (12), aged 5â€“6 years (body condition score: 5â€“6 of 10) were selected with uniform anestrus status and randomly divided into 2 groups namely treatment (Gr I) and control (Gr II), each group consist 6 cows. Anestrus cows in Gr 1 were injected with kisspeptin @ 1.30 Î¼g per kg body weight at 3 day interval for 21 days after 90â€“100 days of post parturition whereas in control group, normal saline was injected as placebo. Blood samples were collected on the days of injection and at estrus in both the groups. Trans-rectal ultrasonography was done at 3 days interval till onset of estrus and ovulation to study the follicular development. Exogenous kisspeptin administration caused significantly early resumption of cyclicity in treatment as compared to control (24.64Â±10.43 vs 66.56Â±14.66 days) and significantly increased kiss1 and GPR54 mRNA expression in treatment as compared to control on the day of estrus (1.943Â±0.29 vs 0.424Â±0.062 and 1.84Â±0.31 vs 0.416Â±0.082, respectively). Similarly, circulating level of estradiol and follicle stimulating hormone (FSH) increased gradually after exogenous administration of kisspeptin, which reached peak on the day of estrus in treatment group (25.36Â±1.27 pg/ml and 15.65Â±1.22 ng/ml, respectively) whereas no significant difference was observed between days of treatment in control group except on the day of estrus (11.29Â±1.76 pg/ml and 9.86Â±1.06 ng/ml, respectively). Level of estradiol and FSH on the day of estrus was significantly higher in treatment as compared to control whereas non-significant difference was observed in plasma progesterone concentration. Number of medium and large follicles increased in treatment whereas only small follicles were observed in control group. Improved endocrinological profiles, follicle development and kiss gene profiles in post partum anestrous mithun cows following exogenous kisspeptin indicates that kisspeptin inducted or resumed the cyclicity in early.
Simulating change in soil organic carbon in two long term fertilizer experiments in India: with the RothC model

Global importance of total organic carbon (TOC) in soil is due to its role in the global carbon cycle and, thus, the part it plays in influencing the atmospheric levels of greenhouse gases (GHGs). It is also of local importance as it determines ecosystem and agro-ecosystem function, influencing various soil parameters. The objective of the present study was to evaluate RothC model (26.3) to estimate TOC changes under two long term fertilizer experimental (LTFE) sites representing humid (Mohanpur) and semi-arid (Akola) climate in India. Five treatments each at the LTFE sites of Mohanpur representing alluvial soils (Alfisol) of the Indo-Gangetic Plains (IGP) and of Akola dominated by black soils (Vertisols) in the Black Soil Region (BSR) were selected. Mohanpur site was modelled for 3 layers (0–13, 13–23, and 23–39 cm) while Akola for surface layer (0–15 cm) only. The root mean square error (RMSE), considered as modelling error, ranged from 3.34 to 17.85%, 6.87 to 14.22% and 6.66 to 24.34% in the 3 soil layers of Mohanpur site respectively. For Akola, RMSE ranges from 1.79 to 13.28. The simulation biases expressed by M (relative error) for all treatments at these sites were non-significant. In Mohanpur, observed trends in TOC show marginal increase in control (T1) and 100% NPK (T2) while the increase was nearly 17 to 35% when organic amendments were applied along with inorganics during 1999 to 2010. For the same period in Akola, control (T1), 50% NPK (T2), and 100% NPK (T4) recorded decrease in TOC while 26 to 29% increase was registered without inorganics and with the combination of inorganics and organics respectively. The calculation of modelled TOC stock to find out effect of global warming indicated that treating the entire soil pedon as a homogenous unit will overestimate effects of global warming in accelerating decomposition of soil carbon.
Two-Dimensional Mixing, Edge Formation, and Permeability Diagnosed in an Area Coordinate

Abstract Two-dimensional mixing of a tracer is diagnosed using A, the area between the tracer contour and a reference contour, as the horizontal coordinate. In the absence of sources or sinks, the tracer distribution in the area coordinate is governed bywhereis the square of the equivalent length of the tracer contour and k is the constant microscale diffusion coefficient. While diffusion is necessary to invoke transport, the large-scale kinematics regulate the evolution of q through the stretching and redistribution of Le2. It is argued that Le2 is a useful, easy to compute diagnostic for irreversible transport, especially for identifying a barrier. Typical behaviors of Le2 in various flow regimes are illustrated using the numerically simulated Kelvin-Helmholtz billow. Signature of mixing is found in the irreversible growth of Le2, but the precise time dependence is complex due to interplay between advection and diffusion. Formation of the edges (concentrated gradients) and their permeability to mass are.
The role of neutrophil–albumin ratio in the diagnosis of acute appendicitis and its efficacy in predicting perforation

ABSTRACT BACKGROUND: Acute appendicitis (AA) is one of the most common causes of abdominal pain in patients presenting to the emergency department with abdominal pain, and despite developments in radiological imaging for its diagnosis, researchers are still in pursuit of a simpler, cheaper, and safer biomarker. Our study investigated the usability of the neutrophil–albumin ratio, a biomarker that predicts prognosis in cases with severe inflammation, in diagnosing AA and anticipating perforation. METHODS: Our study is a retrospective and cross-sectional study. The study was conducted with patients who presented to the emergency department between January 2018 and December 2020 and were hospitalized with a preliminary diagnosis of AA. The cases were first divided into two groups as “Not appendicitis” and “AA,” and then the patients with “AA” were divided into two subgroups as “Perforated” and “Non-perforated. ” The demographic data of the patients, their symptoms, physical examination findings, and the decision-making process for surgery were noted. The neutrophil count and albumin levels detected in the blood samples obtained at the time of admission to the emergency department were noted. Afterward, the neutrophil-albumin ratio (NAR) was calculated and the cutoff level was determined to predict the diagnosis of AA and the development of perforation. RESULTS: The rate of complaints of pain in the periumbilical region was significantly higher in the patient group without AA compared to the patients in the AA group (70.6% and 40.3%, p=0.034). Although the leukocyte count, neutrophil count and percentage, and NAR were significantly higher in the AA group, the lymphocyte count was found to be significantly lower (p=0.005). However, no significant correlation was found between the NAR and the development of perforation (p=0.697). CONCLUSION: The NAR is useful for the diagnosis of AA. Nevertheless, it is not a sufficient biomarker for detecting perforation.
Changing Features of Liver Injury in COVID-19 Patients: Impact of Infection with the SARS-CoV-2 Delta (B.1.617.2) Variants

Background There is growing evidence that abnormal liver function tests (LFTs) are common in patients with coronavirus disease 2019 (COVID-19). However, it is not known whether viral involvement in the liver differs according to the strain. We investigated the impact on liver injury in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta (B.1.617.2) variants. Materials and Methods We conducted a single-center, retrospective cohort study, including 372 patients admitted during the pre-Delta period (PDP: between February 1 and November 30, 2020) and 137 patients admitted during the Delta period (DP: between August 1 and August 31, 2021). Initial liver injury was defined as alanine aminotransferase (ALT) or aspartate aminotransferase (AST) levels ≥3 × the upper limit of normal (ULN) or alkaline phosphatase (ALP) or total bilirubin ≥2 × the ULN within 3 days from admission. Results Of 509 patients with COVID-19 included in our study, 38 (7.5%) patients had initial liver injury. The DP group had a significantly higher rate of initial liver injury than the PDP group (PDP: 5.9% vs. DP: 11.7%, P = 0.028). The DP group (adjusted odds ratio [aOR]: 2.737, 95% confidence interval [CI]: 1.322 – 5.666) was independently associated with initial liver injury. During hospitalization, 160 (31.4%) patients had severe COVID-19. The DP group and initial liver injury had higher odds of progressing to severe COVID-19 (aOR: 2.664, 95% CI: 1.526 - 4.648, and aOR: 4.409, 95% CI: 1.816 - 10.707, respectively). The mediation analysis suggested that initial liver injury mediates the relationship between SARS-CoV-2 Delta variant infection and severe COVID-19 (unstandardized beta coefficient = 0.980, Standard error = 0.284, P = 0.001). Conclusion Initial liver injury is more common in COVID-19 patients with Delta variants. Also, Delta variants and initial liver injury are associated with poor clinical outcomes.
Feature-based human motion parameter estimation with radar

Radar can be an extremely useful sensing technique to observe persons. It perceives persons behind walls or at great distances and in situations where persons have no or poor visibility. Human motion modulates the radar signal which can be observed in the spectrogram of the received signal. Extraction of these movements enables the animation of a person in virtual reality. The authors focus on a fast feature-based approach to estimate human motion features for real-time applications. The human walking model of Boulic is used, which describe the human motion with three parameters. Personification information is obtained by estimating the individual leg and torso parameters. These motion parameters can be estimated from the temporal maximum, minimum and centre velocity of the human motion distribution. Three methods are presented to extract these velocities. Additionally, we extract an independent human motion repetition frequency estimate based on velocity slices in the spectrogram. Kalman filters smooth the parameters and estimate the global Boulic parameters. These estimated parameters are input to the human model of Boulic which forms the basis for animation. The methods are applied to real radar measurements. The animated person generated with the extracted parameters provides a realistic look-alike of the real motion of the person.
Developing human tissue engineered arterial constructs to simulate human in vivo thrombus formation

Abstract Thrombus formation is highly dependent upon the physico-chemical environment in which it is triggered. Our ability to understand how thrombus formation is initiated, regulated, and resolved in the human body is dependent upon our ability to replicate the mechanical and biological properties of the arterial wall. Current in vitro thrombosis models principally use reductionist approaches to model the complex biochemical and cellular milieu present in the arterial wall, and so researcher have favored the use of in vivo models. The field of vascular tissue engineering has developed a range of techniques for culturing artificial human arteries for use as vascular grafts. These techniques therefore provide a basis for developing more sophisticated 3D replicas of the arterial wall that can be used in in vitro thrombosis models. In this review, we consider how tissue engineering approaches can be used to generate 3D models of the arterial wall that improve upon current in vivo and in vitro approaches. We consider the current benefits and limitations of reported 3D tissue engineered models and consider what additional evidence is required to validate them as alternatives to current in vivo models.
Cancer-Associated Fibroblast Diversity Shapes Tumor Metabolism in Pancreatic Cancer

Simple Summary Cancer-associated fibroblasts (CAFs) represent an important stromal cell population of pancreatic cancer in which multiple CAF subtypes have been identified. CAFs engage in a bidirectional crosstalk with cancer cells, continuously adapting their metabolism to external factors, such as chemotherapy. In this review, we summarize recently identified CAF subtypes in pancreatic cancer and discuss how CAFs shape cancer cell metabolism through several mechanisms, notably metabolite exchange, paracrine signaling, desmoplasia/hypoxia and acidosis. Abstract Despite extensive research, the 5-year survival rate of pancreatic cancer (PDAC) patients remains at only 9%. Patients often show poor treatment response, due partly to a highly complex tumor microenvironment (TME). Cancer-associated fibroblast (CAF) heterogeneity is characteristic of the pancreatic TME, where several CAF subpopulations have been identified, such as myofibroblastic CAFs (myCAFs), inflammatory CAFs (iCAFs), and antigen presenting CAFs (apCAFs). In PDAC, cancer cells continuously adapt their metabolism (metabolic switch) to environmental changes in pH, oxygenation, and nutrient availability. Recent advances show that these environmental alterations are all heavily driven by stromal CAFs. CAFs and cancer cells exchange cytokines and metabolites, engaging in a tight bidirectional crosstalk, which promotes tumor aggressiveness and allows constant adaptation to external stress, such as chemotherapy. In this review, we summarize CAF diversity and CAF-mediated metabolic rewiring, in a PDAC-specific context. First, we recapitulate the most recently identified CAF subtypes, focusing on the cell of origin, activation mechanism, species-dependent markers, and functions. Next, we describe in detail the metabolic crosstalk between CAFs and tumor cells. Additionally, we elucidate how CAF-driven paracrine signaling, desmoplasia, and acidosis orchestrate cancer cell metabolism. Finally, we highlight how the CAF/cancer cell crosstalk could pave the way for new therapeutic strategies.
“TYC” Reaction between Alkynes and Catechol-Thiol Derivatives Prompted by Metal Nanocatalysis: Mechanism Study by DFT Calculation

: A recently published article by our research group demonstrated that the hydrothiolation of activated alkynes is a successful way to functionalize thiols bearing catechols. The reaction was promoted by the CuNPs/TiO 2 nanocatalyst and was regio-and stereoselective towards the anti - Markovnikov Z -vinyl sulﬁde with good to excellent yields (47–97%). The scope of the reaction was evaluated and, based on experimental observations, the reaction mechanism was investigated through DFT studies. Theoretical results and experimental data were consistent with a reaction mechanism based on a copper-catalyzed anti -Markovnikov hydrothiolation process that favors the formation of the Z -vinyl sulﬁde.
The lubrication force between two viscous drops

The hydrodynamic force resisting the relative motion of two unequal drops moving along their line of centers is determined for Stokes flow conditions. The drops are assumed to be in near‐contact and to have sufficiently high interfacial tension that they remain spherical. The squeeze flow in the narrow gap between the drops is analyzed using lubrication theory, and the flow within the drops near the axis of symmetry is analyzed using a boundary integral technique. The two flows are coupled through the nonzero tangential stress and velocity at the interface. Depending on the ratio of drop viscosity to that of the continuous phase, and also on the ratio of the distance between the drops to their reduced radius, three possible flow situations arise, corresponding to nearly rigid drops, drops with partially mobile interfaces, and drops with fully mobile interfaces. The results for the resistance functions are in good agreement with an earlier series solution using bispherical coordinates. These results have i.
Non-dominated archiving multi-colony ant algorithm for multi-objective optimization: Application to multi-purpose reservoir operation

Multi-objective optimization using heuristic methods has been established as a subdiscipline that combines the fields of heuristic computation and classical multiple criteria decision making. This article presents the Non-dominated Archiving Ant Colony Optimization (NA-ACO), which benefits from the concept of a multi-colony ant algorithm and incorporates a new information-exchange policy. In the proposed information-exchange policy, after a given number of iterations, different colonies exchange information on the assigned objective, resulting in a set of non-dominated solutions. The non-dominated solutions are moved into an offline archive for further pheromone updating. Performance of the NA-ACO is tested employing two well-known mathematical multi-objective benchmark problems. The results are promising and compare well with those of well-known NSGA-II algorithms used in real-world multi-objective-optimization problems. In addition, the optimization of reservoir operating policy with multiple objectives (i.e. flood control, hydropower generation and irrigation water supply) is considered and the associated Pareto front generated.
Hollow waveguides as polarization converting elements: a theoretical study

Subwavelength apertures in a metallic film act as hollow waveguides. By using a non-quadratic cross-section, an anisotropic transmission behaviour results for the two polarization states. Thus, an array of metallic subwavelength apertures may be used as polarization converter, e.g., as a half-wave plate. By varying orientation and shape of the cross-sections locally, one can design polarization shifting elements for complex wave fields. Here, we present a theoretical consideration on the physical properties and compare with dielectric form birefringence.
The generalization of Lagrange's expansion and the enumeration of trees

In a previous paper ((8)), the joint probability generating function was written down for the sizes of the generations in a tree consisting of c distinct species or colours. It was pointed out in (12) that a generalization of Lagrange's expansion could be applied in order to obtain explicit formulae in some circumstances. The techniques have received new application, to polymer chemistry, in (16), (15), and (17). This application should not be confused with the application of the enumeration of trees to that of isomers ((4), (2), (26)). It was further pointed out in (12) that the technique could be applied to some problems concerning the enumeration of ‘ordered’ (planar) trees. This idea is here developed further, and is applied also to ‘labelled’ trees ‘ordered within colours’. One of our objectives is to specify some circumstances in which iterated generating functions, and consequently also the generalization of Lagrange's expansion, are applicable. The examples show how the techniques can be applied almost automatically in order to deduce results, both old and new. The number of labelled ‘chromatic’ trees with c colours is found: Scoins gave the result for c = 2.
The role of liver resection in the management of severe blunt liver trauma

ABSTRACT BACKGROUND: The management of hepatic trauma has a historical progress from mandatory operation with selective non-operative treatment, to non-operative treatment with selective operation. Liver resection (LR) seems to have a minimal role in the management of liver injury. However, surgical treatment becomes the only life-saving treatment in cases with severe liver trauma. METHODS: It is a retrospective presentation of five cases with severe blunt liver injury whose were admitted at our center during the 8-year period. RESULTS: The median age of patients was 30.8 (23–43). The most frequent mechanism of injury was pedestrian struck (60%). Two of five cases were transferred to our hospital from rural state hospitals after initial attempt to achieving hemostasis. The majority of liver injury was grade V (80%). The right lobe of the liver was injured in different extensions. Major vascular injury was associated to liver injury in four of five cases. The right hepatectomy (n=1), resectional debridement of segments 5, 6, and 7 (n=1), posterior sectorectomy (n=2), and segment 7 resection (n=1) were performed for hemostasis. Vascular injuries in the junction of inferior vena cava and right hepatic vein (n=1), the anterior surface of the right hepatic vein (n=1), the junction of segment 7 hepatic vein and right hepatic vein (n=1), the main portal vein (n=1), and the right renal vein (n=1) were repaired. Median operation time was 162 min (120–180 min). Operative mortality was 20%. Reoperation was needed in three of four survived cases. In-hospital complications were observed in two of four survived cases. Median stay in intensive care unit and hospital was 12.4 days (1–48 days) and 28.2 days (1–65 days), respectively. CONCLUSION: When a severe liver injury is unresponsive to packing, the surgeon must always keep in mind that extensive maneuvers for vascular control and LR are required for bleeding control.
Sp1 Controls the Basal Level of Interleukin-34 Transcription

ABSTRACT Interleukin-34 (IL-34) is a cytokine that plays important roles at steady state and in diseases. The induced or inhibited expression of IL-34 by stimuli has been deeply investigated. However, the regulation of IL-34 basal expression is largely unknown. The aim of this study is to investigate whether IL-34 expression is regulated by a general transcription factor Specificity Protein 1 (Sp1) at transcription level. By using bioinformatic software, four putative Sp1-binding sites overlapping GC boxes were found in the core promoter region of IL-34. Alignment of the core promoter sequences of mammalian IL-34 showed GC box-C (−62/–57) and D (−11/–6) were conserved in some mammals. Luciferase assay results showed that only deletion of GC box-C (−62/–57) significantly reduced luciferase activities of IL-34 core promoter in SH-SY5Y cells. By using electrophoretic mobility shift assay (EMSA), it was found that Sp1 specifically interacted with GC box-C sequence CCCGCC (−62/–57) in the core promoter of IL-34. By using chromatin immunoprecipitation (ChIP), it was discovered that Sp1 bound to the core promoter of IL-34 in living cells. In addition, silencing of Sp1 expression by its specific siRNA reduced IL-34 mRNA and protein levels significantly in SH-SY5Y cells. Likewise, IL-34 expression was inhibited in a dose-dependent manner by a Sp1 inhibitor Plicamycin. Furthermore, silencing of Sp1 also downregulated mRNA and protein expression of IL-34 in GES-1 and 293T cell lines, suggesting that IL-34 transcription regulated by Sp1 was not cell-type specific. Taken together, these results indicate that Sp1 controls the basal level of IL-34 transcription.