Miracle-dz/UniFAR-FTUs huggingface dataset:
Improving the Clinical Value and Utility of CGM Systems: Issues and Recommendations

The first systems for continuous glucose monitoring (CGM) became available over 15 years ago. Many then believed CGM would revolutionize the use of intensive insulin therapy in diabetes; however, progress toward that vision has been gradual. Although increasing, the proportion of individuals using CGM rather than conventional systems for self-monitoring of blood glucose on a daily basis is still low in most parts of the world. Barriers to uptake include cost, measurement reliability (particularly with earlier-generation systems), human factors issues, lack of a standardized format for displaying results, and uncertainty on how best to use CGM data to make therapeutic decisions. This Scientific Statement makes recommendations for systemic improvements in clinical use and regulatory (pre- and postmarketing) handling of CGM devices. The aim is to improve safety and efficacy in order to support the advancement of the technology in achieving its potential to improve quality of life and health outcomes for more people with diabetes.
The Seeding and Cultivation of a Tropical Species of Filamentous Ulva for Algal Biomass Production

Filamentous species of Ulva are ideal for cultivation because they are robust with high growth rates and maintained across a broad range of environments. Temperate species of filamentous Ulva are commercially cultivated on nets which can be artificially ‘seeded’ under controlled conditions allowing for a high level of control over seeding density and consequently biomass production. This study quantified for the first time the seeding and culture cycle of a tropical species of filamentous Ulva (Ulva sp. 3) and identified seeding density and nursery period as key factors affecting growth and biomass yield. A seeding density of 621,000 swarmers m-1 rope in combination with a nursery period of five days resulted in the highest growth rate and correspondingly the highest biomass yield. A nursery period of five days was optimal with up to six times the biomass yield compared to ropes under either shorter or longer nursery periods. These combined parameters of seeding density and nursery period resulted in a specific growth rate of more than 65% day−1 between 7 and 10 days of outdoor cultivation post-nursery. This was followed by a decrease in growth through to 25 days. This study also demonstrated that the timing of harvest is critical as the maximum biomass yield of 23.0±8.8 g dry weight m−1 (228.7±115.4 g fresh weight m−1) was achieved after 13 days of outdoor cultivation whereas biomass degraded to 15.5±7.3 g dry weight m−1 (120.2±71.8 g fresh weight m−1) over a longer outdoor cultivation period of 25 days. Artificially seeded ropes of Ulva with high biomass yields over short culture cycles may therefore be an alternative to unattached cultivation in integrated pond-based aquaculture systems.
An Alternative Test of Tackling Ability in Rugby League Players.

This study investigated the relationship between 2 tests of tackling ability, muscle strength, and power in semiprofessional rugby league players. Thirty-one players, 19 first-grade and 12 second-grade, underwent tests of muscle strength (1-repetition-maximum bench press, chin-up, and squat) and power (plyometric push-up and countermovement jump). Tackling ability was assessed via video analysis of under-and over-the-ball tackle drills. The first-grade players had significantly greater scores in both the under-the-ball (P = .03, effect size [ES] = 0.84, 95% CI 0.07-1.50) and over-the-ball tackling-ability tests (P < .001, ES =1.86, 95% CI 0.83-2.52) than the second-grade players. A large, significant relationship was found between under- and over-the-ball tackling ability (r = .55, 95% CI .24-.76, P = .001). Lower-body strength (r = .37, 95% CI .02-.64, P = .04) was moderately associated with under-the-ball tackling ability, whereas over-the-ball tackling ability was moderately associated with plyometric push-up performance (r = .39, 95% CI .04-.65, P = .03). This study found that over-the-ball tackling ability was significantly associated with under-the-ball tackling in semiprofessional rugby league players. Furthermore, it was found that, compared with the second-grade players, the first-grade players had superior tackle ability in both tackle drills. In this study it was observed that plyometric push-up peak power was significantly related to over-the-ball tackling ability and absolute lower-body strength was associated with under-the-ball tackling ability. These findings provide skill coaches and strength and conditioning staff a greater understanding of elements that contribute to effective tackling ability.
Identification of Novel Immunogenic Proteins from Mycoplasma bovis and Establishment of an Indirect ELISA Based on Recombinant E1 Beta Subunit of the Pyruvate Dehydrogenase Complex

The pathogen Mycoplasma bovis (M. bovis) is a major cause of respiratory disease, mastitis, and arthritis in cattle. Screening the key immunogenic proteins and updating rapid diagnostic techniques are necessary to the prevention and control of M. bovis infection. In this study, 19 highly immunogenic proteins from M. bovis strain PD were identified using 2-dimensional gel electrophoresis, immunoblotting and MALDI-TOF/TOF MS. Of these 19 proteins, pyruvate dehydrogenase E1 component beta subunit (PDHB) showed excellent immune reactivity and repeatability. PDHB was found to be conserved in different M. bovis isolates, as indicated by Western blot analysis. On the basis of these results, a rPDHB-based indirect ELISA (iELISA) was established for the detection of serum antibodies using prokaryotically expressed recombinant PDHB protein as the coating antigen. The specificity analysis result showed that rPDHB-based iELISA did not react with other pathogens assessed in our study except M. agalactiae (which infects sheep and goats). Moreover, 358 serum samples from several disease-affected cattle feedlots were tested using this iELISA system and a commercial kit, which gave positive rates of 50.8% and 39.9%, respectively. The estimated Kappa agreement coefficient between the two methods was 0.783. Notably, 39 positive serum samples that had been missed by the commercial kit were all found to be positive by Western blot analysis. The detection rate of rPDHB-based iELISA was significantly higher than that of the commercial kit at a serum dilution ratio of 1∶5120 to 1∶10,240 (P<0.05). Taken together, these results provide important information regarding the novel immunogenic proteins of M. bovis. The established rPDHB-based iELISA may be suitable for use as a new method of antibody detection in M. bovis.
Customized Corneal Cross-linking Using Different UVA Beam Profiles.

PURPOSE To evaluate the performance of different customized corneal cross-linking (CXL) methods. METHODS This was a single-center interventional, prospective, longitudinal case series. Four different customized CXL methods were evaluated in keratoconic eyes: (1) uniform (uniform intensity ultraviolet-A [UVA] beam [9 mW/cm2] for 10 minutes) (n = 12 eyes); (2) sector axial map (sector-based UVA irradiation) (n = 12 eyes); (3) ring axial map (concentric rings of UVA beam intensity centered at the steepest curvature of the anterior axial map) (n = 12 eyes); and ring tangential map (same as the ring axial map but centered at the steepest curvature of the anterior tangential map) (n = 14 eyes). Peak UVA energy density in the sector and ring axial map (and ring tangential map) protocols did not exceed 15.0 and 10.8 J/cm2, respectively. A 0.1% riboflavin solution was applied after epithelium removal. Corneal tomography and visual acuity were assessed before and 6 months after CXL. RESULTS Average and peak energy density was lowest in the ring tangential protocol and highest in the sector axial map group (P < .001). Treated area was lowest in the ring tangential map group and highest in the uniform group (P < .001). Decrease in curvature was similar among the uniform, sector axial map, and ring axial map groups (P < .05). The ring tangential map group had the greatest decrease in curvature per unit energy dose to the cornea (P < .05). Improvement in uncorrected (0.081 ± 0.056 logMAR) and corrected (0.041 ± 0.026 logMAR) distance visual acuity per unit energy density was greatest in the ring tangential map group (P > .05). CONCLUSIONS When normalized to the average energy density, the ring tangential map protocol appeared to provide maximum flattening and improvement in visual acuity. Further studies with larger sample sizes are needed to validate the findings of this pilot study. [J Refract Surg. 2017;33(10):676-682. ].
Composite processing and property evaluation of far-infrared/electromagnetic shielding bamboo charcoal/phase change material/stainless steel elastic composite fabrics

Abstract This study aims to fabricate far-infrared (FIR)/electromagnetic shielding composite fabric and its composite yarn. Five types of composite yarns with different sheath components were made by using bamboo charcoal (BC) fibers, phase change material (PCM) roving and stainless steel (SS) fibers via a ring spinning frame, and then fabricated into five elastic warp-knitted fabrics with different weft yarns using a crochet knitting machine. The mechanical properties of different constituents of composite yarns and their fabrics, as well as FIR emissivity and electromagnetic shielding effectiveness (EMSE) of resulting fabrics were evaluated. The results show that BC/SS composite yarns and their fabricated warp-knitted fabrics display the highest tensile strength. Warp-knitted fabrics containing BC fibers possess the highest FIR emissivity. EMSE of the fabricated warp-knitted fabrics improves proportionally with the number of the lamination layers. The resulting multifunctional elastic knitted fabrics apply as athletic clothing, underwear, socks, protective or healthcare products in the future.
Surgical Versus Nonoperative Treatment for Lumbar Disc Herniation: Eight-Year Results for the Spine Patient Outcomes Research Trial

Study Design. Concurrent prospective randomized and observational cohort studies. Objective. To assess the 8-year outcomes of surgery versus nonoperative care. Summary of Background Data. Although randomized trials have demonstrated small short-term differences in favor of surgery, long-term outcomes comparing surgical with nonoperative treatment remain controversial. Methods. Surgical candidates with imaging-confirmed lumbar intervertebral disc herniation meeting Spine Patient Outcomes Research Trial eligibility criteria enrolled into prospective randomized (501 participants) and observational cohorts (743 participants) at 13 spine clinics in 11 US states. Interventions were standard open discectomy versus usual nonoperative care. Main outcome measures were changes from baseline in the SF-36 Bodily Pain and Physical Function scales and the modified Oswestry Disability Index-AAOS/Modems version assessed at 6 weeks, 3 months, and 6 months, and annually thereafter. Results. Advantages were seen for surgery in intent-to-treat analyses for the randomized cohort for all primary and secondary outcomes other than work status; however, with extensive nonadherence to treatment assignment (49% patients assigned to nonoperative therapy receiving surgery versus 60% of patients assigned to surgery) these observed effects were relatively small and not statistically significant for primary outcomes (bodily pain, physical function, Oswestry Disability Index). Importantly, the overall comparison of secondary outcomes was significantly greater with surgery in the intent-to-treat analysis (sciatica bothersomeness [P > 0.005], satisfaction with symptoms [P > 0.013], and self-rated improvement [P > 0.013]) in long-term follow-up. An as-treated analysis showed significant surgical treatment effects for primary outcome measures (mean change, surgery vs. nonoperative care; treatment effect; 95% confidence interval): bodily pain (45.3 vs. 34.4; 10.9; 7.7 to 14); PF (42.2 vs. 31.5; 10.6; 7.7 to 13.5); and Oswestry Disability Index (−36.2 vs. −24.8; −11.3; −13.6 to −9.1). Conclusion. Carefully selected patients who underwent surgery for a lumbar disc herniation achieved greater improvement than nonoperatively treated patients; there was little to no degradation of outcomes in either group (operative and nonoperative) from 4 to 8 years. Level of Evidence: 2
Importance of Saprotrophic Freshwater Fungi for Pollen Degradation

Fungi and bacteria are the major organic matter (OM) decomposers in aquatic ecosystems. While bacteria are regarded as primary mineralizers in the pelagic zone of lakes and oceans, fungi dominate OM decomposition in streams and wetlands. Recent findings indicate that fungal communities are also active in lakes, but little is known about their diversity and interactions with bacteria. Therefore, the decomposer niche overlap of saprotrophic fungi and bacteria was studied on pollen (as a seasonally recurring source of fine particulate OM) by performing microcosm experiments with three different lake types. Special emphasis was placed on analysis of fungal community composition and diversity. We hypothesized that (I) pollen select for small saprotrophic fungi and at the same time for typical particle-associated bacteria; (II) fungal communities form specific free-living and attached sub-communities in each lake type; (III) the ratio between fungi or bacteria on pollen is controlled by the lake's chemistry. Bacteria-to-fungi ratios were determined by quantitative PCR (qPCR), and bacterial and fungal diversity were studied by clone libraries and denaturing gradient gel electrophoresis (DGGE) fingerprints. A protease assay was used to identify functional differences between treatments. For generalization, systematic differences in bacteria-to-fungi ratios were analyzed with a dataset from the nearby Baltic Sea rivers. High abundances of Chytridiomycota as well as occurrences of Cryptomycota and yeast-like fungi confirm the decomposer niche overlap of saprotrophic fungi and bacteria on pollen. As hypothesized, microbial communities consistently differed between the lake types and exhibited functional differences. Bacteria-to-fungi ratios correlated well with parameters such as organic carbon and pH. The importance of dissolved organic carbon and nitrogen for bacteria-to-fungi ratios was supported by the Baltic Sea river dataset. Our findings highlight the fact that carbon-to-nitrogen ratios may also control fungal contributions to OM decomposition in aquatic ecosystems.
Experimental and analytical evaluation of a biaxial test for determining in-plane shear properties of composites

The results of an experimental and analytical investigation of a biaxial tension-compression test for determining shear properties of composite materials are presented. A fixture was designed to introduce an equal and opposite pair of forces into a cruciform like specimen. The cruciform specimen was designed using finite element analysis. Carbon-epoxy composite ([90/0] 2S and [±45] 2S ) and aluminum specimens were tested in the fixture. The finite element analysis and strains measured during the tests showed that the stress state in the center of a cruciform specimen with large corner radii was pure shear. Stress gradients near the boundary of the test section were small, and the optimal specimen geometry was independent of laminate orientation. Shear strength was less than anticipated because failure initiated due to instability or due to a complex inplane and interlaminar stress state at the specimen boundaries
Parasite dynamics in an invaded ecosystem: helminth communities of native wood mice are impacted by the invasive bank vole

SUMMARY It is becoming increasingly evident that biological invasions result in altered disease dynamics in invaded ecosystems, with knock-on effects for native host communities. We investigated disease dynamics in an invaded ecosystem, using the helminth communities of the native wood mouse (Apodemus sylvaticus) in the presence and absence of the invasive bank vole (Myodes glareolus) in Ireland. Native wood mice were collected over 2 years from four sites to assess the impact of the presence of the bank vole on wood mouse helminth community dynamics both at the component and infracommunity level. We found evidence for dilution (Syphacia stroma), spill-back (Aonchotheca murissylvatici) and spill-over (Taenia martis) in native wood mice due to the presence of the bank vole. Site of capture was the most important factor affecting helminth community structure of wood mice, along with year of capture and host-age and the interactions between them.
Semi-supervised network inference using simulated gene expression dynamics

Motivation Inferring the structure of gene regulatory networks from high-throughput datasets remains an important and unsolved problem. Current methods are hampered by problems such as noise, low sample size, and incomplete characterizations of regulatory dynamics, leading to networks with missing and anomalous links. Integration of prior network information (e.g. from pathway databases) has the potential to improve reconstructions. Results We developed a semi-supervised network reconstruction algorithm that enables the synthesis of information from partially known networks with time course gene expression data. We adapted partial least square-variable importance in projection (VIP) for time course data and used reference networks to simulate expression data from which null distributions of VIP scores are generated and used to estimate edge probabilities for input expression data. By using simulated dynamics to generate reference distributions, this approach incorporates previously known regulatory relationships and links the network to the dynamics to form a semi-supervised approach that discovers novel and anomalous connections. We applied this approach to data from a sleep deprivation study with KEGG pathways treated as prior networks, as well as to synthetic data from several DREAM challenges, and find that it is able to recover many of the true edges and identify errors in these networks, suggesting its ability to derive posterior networks that accurately reflect gene expression dynamics. Availability and implementation R code is available at https://github.com/pn51/postPLSR. Contact rbraun@northwestern.edu. Supplementary information Supplementary data are available at Bioinformatics online.
Prioritizing Populations for Conservation Using Phylogenetic Networks

In the face of inevitable future losses to biodiversity, ranking species by conservation priority seems more than prudent. Setting conservation priorities within species (i.e., at the population level) may be critical as species ranges become fragmented and connectivity declines. However, existing approaches to prioritization (e.g., scoring organisms by their expected genetic contribution) are based on phylogenetic trees, which may be poor representations of differentiation below the species level. In this paper we extend evolutionary isolation indices used in conservation planning from phylogenetic trees to phylogenetic networks. Such networks better represent population differentiation, and our extension allows populations to be ranked in order of their expected contribution to the set. We illustrate the approach using data from two imperiled species: the spotted owl Strix occidentalis in North America and the mountain pygmy-possum Burramys parvus in Australia. Using previously published mitochondrial and microsatellite data, we construct phylogenetic networks and score each population by its relative genetic distinctiveness. In both cases, our phylogenetic networks capture the geographic structure of each species: geographically peripheral populations harbor less-redundant genetic information, increasing their conservation rankings. We note that our approach can be used with all conservation-relevant distances (e.g., those based on whole-genome, ecological, or adaptive variation) and suggest it be added to the assortment of tools available to wildlife managers for allocating effort among threatened populations.
Y90 Radioembolization Significantly Prolongs Time to Progression Compared With Chemoembolization in Patients With Hepatocellular Carcinoma.

BACKGROUND & AIMS Conventional transarterial chemoembolization (cTACE) is used to treat patients with hepatocellular carcinoma (HCC). Radioembolization is a minimally invasive procedure that involves implantation of radioactive micron-sized particles loaded with yttrium-90 (Y90) inside the blood vessels that supply a tumor. We performed a randomized, phase 2 study to compare the effects of cTACE and Y90 radioembolization in patients with HCC. METHODS From October 2009 through October 2015, we reviewed patients with HCC of all Barcelona Clinic Liver Cancer (BCLC) stages for eligibility. Of these, 179 patients with BCLC stages A or B met our enrollment criteria and were candidates for cTACE or Y90 therapy. Patients were assigned randomly to groups that received Y90 therapy (n = 24; 50% Child-Pugh A) or cTACE (n = 21; 71% Child-Pugh A). The primary outcome was time to progression (TTP), evaluated by intention-to-treat analysis. Secondary outcomes included safety, rate of response (based on tumor size and necrosis criteria), and Kaplan-Meier survival time. We performed inverse probability of censoring weighting and competing risk analyses. RESULTS Patients in the Y90 radioembolization group had significant longer median TTP (>26 mo) than patients in the cTACE group (6.8 mo; P = .0012) (hazard ratio, 0.122; 95% confidence interval [CI], 0.027-0.557; P = .007). This was confirmed by competing risk and inverse probability of censoring weighting analyses accounting for transplantation or death. A significantly greater proportion of patients in the cTACE group developed diarrhea (21%) than in the Y90 group (0%; P = .031) or hypoalbuminemia (58% in the cTACE group vs 4% in the Y90 group; P < .001). Similar proportions of patients in each group had a response to therapy, marked by necrosis (74% in the cTACE group vs 87% in the Y90 group) (P = .433). The median survival time, censored to liver transplantation, was 17.7 months for the cTACE group (95% CI, 8.3-not calculable) vs 18.6 months for the Y90 group (95% CI, 7.4-32.5) (P = .99). CONCLUSIONS In a randomized phase 2 study of patients with HCC of BCLC stages A or B, we found Y90 radioembolization to provide significantly longer TTP than cTACE. Y90 radioembolization provides better tumor control and could reduce drop-out from transplant waitlists. ClinicalTrials.gov no. NCT00956930.
Cortical response states for enhanced sensory discrimination

Brain activity during wakefulness is characterized by rapid fluctuations in neuronal responses. Whether these fluctuations play any role in modulating the accuracy of behavioral responses is poorly understood. Here, we investigated whether and how trial changes in the population response impact sensory coding in monkey V1 and perceptual performance. Although the responses of individual neurons varied widely across trials, many cells tended to covary with the local population. When population activity was in a ‘low’ state, neurons had lower evoked responses and correlated variability, yet higher probability to predict perceptual accuracy. The impact of firing rate fluctuations on network and perceptual accuracy was strongest 200 ms before stimulus presentation, and it greatly diminished when the number of cells used to measure the state of the population was decreased. These findings indicate that enhanced perceptual discrimination occurs when population activity is in a ‘silent’ response mode in which neurons increase information extraction.