Miracle-dz/UniFAR-FTUs huggingface dataset:
Culture-Independent Analysis of Gut Bacteria: the Pig Gastrointestinal Tract Microbiota Revisited

ABSTRACT The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total of 24 pigs representing a variety of diets, ages, and herd health status. A library comprising 4,270 cloned 16S rDNA sequences obtained directly by PCR from 52 samples of either the ileum, the cecum, or the colon was constructed. In total, 375 phylotypes were identified using a 97% similarity criterion. Three hundred nine of the phylotypes (83%) had a <97% sequence similarity to any sequences in the database and may represent yet-uncharacterized bacterial genera or species. The phylotypes were affiliated with 13 major phylogenetic lineages. Three hundred four phylotypes (81%) belonged to the low-G+C gram-positive division, and 42 phylotypes (11.2%) were affiliated with the Bacteroides and Prevotella group. Four clusters of phylotypes branching off deeply within the low-G+C gram-positive bacteria and one in the Mycoplasma without any cultured representatives were found. The coverage of all the samples was 97.2%. The relative abundance of the clones approximated a lognormal distribution; however, the phylotypes detected and their abundance varied between two libraries from the same sample. The results document that the intestinal microbial community is very complex and that the majority of the bacterial species colonizing the gastrointestinal tract in pigs have not been characterized.
[Validation of the Spanish version of PRIME-MD: a procedure for diagnosing mental disorders in primary care].

INTRODUCTION Psychiatric disorders occurs in at least 20% of patients attending Primary Care settings, however, only 50% of them are detected by primary care physicians. Therefore a tool is required which can help primary care physicians to detect and diagnose psychiatric disorders. PRIME-MD (Primary Care Evaluation of Mental Disorders) is a questionnaire designed with this aim. In this article the results of the validation study of the Spanish version of this questionnaire are presented. MATERIALS AND METHODS 312 patients were interviewed by primary care physicians using PRIME-MD and by psychiatrists using SCAN (Schedules for Clinical Assessment in Neuropsychiatry). RESULTS The time most frequently spent in questionnaire application by the physician was 10 minutes. PRIME-MD detected the presence of at least one psychiatric disorder in 44.3% of patients. PRIME-MD diagnoses agree well with SCAN diagnoses for mood disorders (coefficient of agreement: 0.50) and for anxiety disorders (coefficient of agreement: 0.35), but not for somatoform disorders or alcohol-related disorders. CONCLUSIONS The Spanish version of PRIME-MD questionnaire in a useful tool to identify and diagnose mood and anxiety disorders in Primary Care settings.
Relation-Aware Volume Exploration Pipeline

Volume exploration is an important issue in scientific visualization. Research on volume exploration has been focused on revealing hidden structures in volumetric data. While the information of individual structures or features is useful in practice, spatial relations between structures are also important in many applications and can provide further insights into the data. In this paper, we systematically study the extraction, representation,exploration, and visualization of spatial relations in volumetric data and propose a novel relation-aware visualization pipeline for volume exploration. In our pipeline, various relations in the volume are first defined and measured using region connection calculus (RCC) and then represented using a graph interface called relation graph. With RCC and the relation graph, relation query and interactive exploration can be conducted in a comprehensive and intuitive way. The visualization process is further assisted with relation-revealing viewpoint selection and color and opacity enhancement. We also introduce a quality assessment scheme which evaluates the perception of spatial relations in the rendered images. Experiments on various datasets demonstrate the practical use of our system in exploratory visualization.
Vasculitis, a Rare Presentation of a Post-Streptococcal

Post-streptococcal vasculitis is not awell-recognized non-suppurative complication of Group A streptococcal infections. Seventeen-year-old male was admitted with palpable purpura in the limbs, ankle swelling, microscopic hematuria and elevated inflammation markers. After short remission period, an extensive exudative pleural effusion was identified. High anti-streptococcal antibodies titers were found. This report illustrates an atypical and rare presentation of a post-streptococcal syndrome.
Ozone-Induced Programmed Cell Death in the Arabidopsis radical-induced cell death1 Mutant1

Short, high-concentration peaks of the atmospheric pollutant ozone (O3) cause the formation of cell death lesions on the leaves of sensitive plants. Numerous similarities between the plant responses to O3 and pathogens suggest that O3 triggers hypersensitive response-like programmed cell death (PCD). We examined O3 and superoxide-induced cell death in the O3-sensitive radical-induced cell death1 (rcd1) mutant. Dying cells in O3-exposed rcd1 exhibited several of the typical morphological characteristics of the hypersensitive response and PCD. Double-mutant analyses indicated a requirement for salicylic acid and the function of the cyclic nucleotide-gated ion channel AtCNGC2 in cell death. Furthermore, a requirement for ATPases, kinases, transcription, Ca2+ flux, caspase-like proteolytic activity, and also one or more phenylmethylsulfonyl fluoride-sensitive protease activities was shown for the development of cell death lesions in rcd1. Furthermore, mitogen-activated protein kinases showed differential activation patterns in rcd1 and Columbia. Taken together, these results directly demonstrate the induction of PCD by O3.
Contenidos obsesivos, miedo a la enfermedad y asco

Recently, data about the associations between disgust and obsessive-compulsive disorder (OCD) have show some inconsistencies, which might be due to the differential role of disgust sensitivity and propensity, the infl uence of other variables, or the heterogeneity of OCD contents. This study examines the relationships among disgust sensitivity and propensity, fear of illness, and different obsessional contents in university students (N = 114). Disgust propensity was the most relevant variable in predicting Doubt/checking contents (19% of explained variance, EV), and fear of illness and disgust sensitivity were the most relevant variables predicting Contamination (EV: 21% and 8%, respectively), and Superstitious contents (EV: 21% and 24%, respectively). Results suggest the need to consider the disgust and fear of illness dimensions in the conceptualization, assess- ment and treatment programs of OCD.
Autobiographical Memory After Temporal Lobe Lesions

Abstract To investigate how the brain organizes and stores autobiographical memory, eight subjects with focal lesions of the temporal lobe and one comparison subject with frontal lobe lesion were studied with the Autobiographical Memory Interview (AMI). The AMI surveys both personal semantic memory and autobiographical incidents from childhood, early adulthood and recent life. Left mesial temporal lobe lesion caused anterograde and time-limited retrograde autobiographical memory changes. More extensive left temporal lobe lesion disrupted personal semantic memory dating back to childhood, but did not impair recollection of autobiographical incidents. However, bilateral temporal lobe lesions caused extensive autobiographical memory deficits which varied in temporal extent according to the particular location of damage. Bilateral prefrontal cortex damage also resulted in severe impairment, which appeared to be related to disorganized retrieval. The findings suggest that many verbal-semantic aspects of autobi.
Hydronephrosis and Urine Retention in Estrogen-Implanted Athymic Nude Mice

Subcutaneous estrogen pellet implantation is commonly practiced to induce tumor promotion in nude (Nu-FOXnl nu ) mice for xenograft tumor modeling. Twelve of 23, 18-week-old nude mice developed dysuria after estrogen pellet implantation. Physical examination revealed variably distended abdomens with palpable distended bladders. All mice were euthanatized and postmortem examinations were made. Blood samples of the mice were collected, and serum estrogen concentration was measured. Postmortem examination revealed variable dilation of the urinary bladder and unilateral or bilateral dilations of ureters and kidneys. Microscopically, 12 of 23 mice and 13 of 23 mice showed dilated urinary bladder and hydronephrosis, respectively.
Bio-inspired motion strategies for a bimanual manipulation task

We consider the complex task of coordinating two five-fingered anthropomorphic robot hands for taking a jar passed from a human user and unscrewing its cap. Using a pair of 7-DOF redundant arms for operating the hands, we study how the incorporation of human movement strategies at the finger and arm levels can aid in the solution of the overall bimanual task. At the finger level, we employ a finger control manifold for the unscrewing motion that has been synthesized with a kernel approach applied to human motion data captured with a data glove. At the arm level, we use a heuristic motivated from the observation of human arm movements to enhance the space of pass-over configurations that the system can successfully handle. In addition, we provide a brief description of the architecture of the overall system that comprises 54 motor degrees of freedom and integrates camera vision, arm and finger control as well as a speech output component for interaction with the human user.
The flavivirus envelope protein E: isolation of a soluble form from tick-borne encephalitis virus and its crystallization

By the use of limited trypsin digestion of purified virions, we generated a membrane anchor-free and crystallizable form of the tick-borne encephalitis virus envelope glycoprotein E. It retained its reactivity with a panel of monoclonal antibodies, and only subtle structural differences from the native protein E were recognized. Treatment with the bifunctional cross-linker dimethylsuberimidate resulted in the formation of a dimer. Crystallization experiments yielded hexagonal rod-shaped crystals suitable for X-ray diffraction analysis.
Five Days at Memorial: Life and Death in a Storm-Ravaged Hospital, by Sheri Fink

copies. The innumerable copies of the scrapie prion in the diseased brain fold to form the amorphous amyloid structures characteristic of neurologic spongiform diseases. Experiments in genetically altered mice proved conclusively that scrapie was caused by a prion. Prusiner also demonstrated that mutations in prion proteins gave rise to familial forms of the human spongiform-associated diseases. Having established in his own mind the basic biology of prions, Prusiner then found himself engaged in battles on two fronts: On one hand, many scientists and science writers were still very skeptical about the whole prion theory; on the other, there were a few investigators who accepted the validity of the concept, but claimed that they, rather than Prusiner, had first conceived of it. Prusiner’s ideas received wider interest following the revelation that some consumers of beef from strangely acting cattle in Britain had come down with a form of CJD at much younger ages than had been previously observed in that disorder, giving rise to the term mad cow disease. The origin of this epidemic was the feeding of cattle with sheep offal from some animals that had scrapie. The prion in turn passed on to and was expressed in a small number of the unfortunate consumers of the scrapie-infected beef. The scientific evidence Prusiner had uncovered and the occurrence of mad cow disease convinced most, but even to this day not all, skeptics that prions really existed and that they were indeed protein in nature. Prusiner received the Lasker Award in 1994 and the 1997 Nobel Prize in Physiology or Medicine. He was the sole winner of the latter award that year, a most unusual honor, both because he had discovered a novel principle of infection and the lonely struggle he had undergone to have his heretical theory accepted. In 2010, Prusiner received the National Medal of Science from President Obama, the highest honor this country awards to scientists. While a few scientists and science writers still have difficulty accepting Prusiner’s concept of prions, there is strong evidence that his research will point the way to understanding and developing treatments for some of our most serious, unsolved problems in neurology and psychiatry.
PHEV Charging and Discharging Cooperation in V2G Networks: A Coalition Game Approach

Recently, plug-in hybrid electric vehicles (PHEVs) have attracted considerable attention as a sustainable transport system and also an essential component of the smart grid. With the rapid growth of PHEVs penetration, the charging and discharging of PHEVs will pose a significant impact on the residential electricity distribution network. For this reason, the management of PHEV charging and discharging has become one of the key issues in the research of PHEVs. In most existing work, PHEVs are supposed to operate individually for charging and discharging in the grid. However, we argue that, by leveraging the cooperation among PHEVs, the grid will efficiently stimulate PHEV users to charge in load valley and discharge in load peak. As a consequence, the electricity load is well balanced. Meanwhile, the PHEV users also achieve higher profit. The PHEV charging and discharging cooperation is a win-win strategy for both the grid and the PHEV users. We formulate and resolve the PHEV charging and discharging cooperation in the framework of coalition game. The simulation results indicate that the peak-valley difference in electricity load of the grid is significantly reduced. Besides, the PHEV users have better satisfaction in the vehicle battery status and the economic profit.
Repressed Memory and other Controversial Origins of Sexual Abuse Allegations: Beliefs among Psychologists and Clinical Social Workers

The purpose of this study was to assess beliefs about repressed memory and other controversial origins of sexual abuse allegations. This was examined by conducting a national survey of psychologists and clinical social workers. The results indicated that experimental psychologists and clinicians differ regarding belief in repressed memory, with clinicians expressing more confidence that such memories can and do exist. No differences were found between clinicians with different types of academic training. However, academic degree and level of research involvement were found to be related to views toward one or more of the following: (a) usefulness of anatomical dolls, (b) leading questions, or (c) the possibility of therapeutic techniques leading to false allegations of sexual abuse. Although the respondents' sex often accounted for a significant portion of the variance, removing this influence did not alter the overall pattern of the results. Possible explanations for these results are discussed.
A Vision for the Future: Collective Effort for Systemic Change

N HER response to my proposal for reconceptualizing Teach for America, Wendy Kopp writes, “We cannot expect every teacher to go above and beyond traditional expectations to the extent necessary to compensate for all the weaknesses of the system. ” Although I agree that, without the proper training, support, and resources, teachers cannot be expected to transform classrooms in our nation’s poorest districts, I also believe that the education community should move toward a system that does expect teachers to go above and beyond the status quo and provides the supports necessary to enable them to do so. The quality of the teacher in the classroom is the most important factor in raising student achievement, as Linda Darling-Hammond noted, and we should offer our students nothing less than well-trained and wellsupported teachers. Providing high-quality teachers is of particular importance in low-income communities of color, where the most underprepared teachers are consistently placed and where students continually underperform in comparison to their middle-class white counterparts. Ms. Darling-Hammond describes the detrimental outcomes of the present system, one that continues to fail its students.
Recentering North American Environmental History: Pedagogy and Scholarship in the Great Lakes Region

We lay out a rationale for a new scholarly subfield—the environmental history of the Great Lakes—that Canadian and U.S. scholars can develop together. This project began as an attempt to teach environmental history in a way that stressed the importance of our region to our Wisconsin- and Michigan-based students. But classroom experiences convinced us that Great Lakes environmental history has been neglected, both because of its transnational character and because of biases within the field of environmental history. The Great Lakes have important stories to tell. They are at once representative of the larger narratives of both the United States and Canada, and also demonstrate the exceptional history of each nation. They provide an opportunity to explore comparative and transnational history. The Great Lakes also are fundamentally important in their own right for their industries, environments, and abundant resources.
THE CHANGING SOCIAL AND DEMOGRAPHIC PROFILE OF NURSING STUDENTS IN NEW SOUTH WALES

ABSTRACT This study had two major aims: 1) to establish a reliable data base on the social and demographic origins of nursing students in New South Wales universities since the introduction of the Higher Education Contribution Scheme (HECS) and the change from diploma to degree level programs; and 2) to compare the social and demographic origins of nursing students in higher education programs in 1995 with the data base established by Wright in New South Wales in 1986 to detect changes over time. Comparison of data from the 1986 group (N=447) and the 1995 group (N=512) revealed that there was a no significant difference between the two cohorts on the socio‐economic variable. There was, however, a significant difference in the SES variable between the 1986 and 1995 sample for the female group. From a household perspective, there was a significant difference in the number of siblings, and income received by the mother between the 1986 and 1995 cohorts. In addition, no support was found for the assertion tha.
Patient safety culture transformation in a children’s hospital: an interprofessional approach

Abstract In 2008, a children’s hospital based in the Midwest of the USA launched a hospital-wide safety transformation initiative to improve the safety and quality of care resulting in a decrease in the number of critical safety incidents. In order to build on the early successes of the Hospital’s safety program and further improve safety metrics, investigators developed a set of multi-pronged, interprofessional interventions designed to improve overall safety outcomes. The interprofessional interventions focused on didactic training, simulation exercises and safety rounding components. Study results indicate that the didactic portion of the study intervention was the most effective component in terms of safety behavior knowledge gained and satisfaction. The student groups had statistically significant higher post-didactic (86.2 versus 77.7, p < 0.001) and post-simulation (85 versus 81.8, p < 0.05) knowledge scores than did the staff groups. After gaining knowledge in basic safety training didactic instruction, students and staff maintained the knowledge gain throughout the study, but no significant knowledge gains were observed after simulation experiences and rounding with safety coaches. An overall increase in hospital metrics (all safety events) of the study year, compared retrospectively to the previous year, was observed. Investigators attribute the increase in the metric indicators to greater attention to reporting safety events.
Cloning of the Serratia marcescens hasF gene encoding the Has ABC exporter outer membrane component: a TolC analogue

The Serratia marcescens haemophore HasA is secreted by an ABC exporter comprising three envelope proteins. The ABC protein (ATP‐binding cassette) HasD and the MFP protein (membrane fusion protein) HasE but not the outer membrane component have been isolated previously. In Escherichia coli, TolC, the outer membrane component of the haemolysin transporter, can form a hybrid exporter with HasD and HasE. This hybrid secretes HasA and the very similar metalloproteases from S. marcescens and Erwinia chrysanthemi. By analogy, the genuine exporter was predicted to secrete metalloproteases. The hasF gene was thus cloned from S. marcescens into an E. coli tolC mutant carrying hasD and hasE genes, by screening for a proteolytic phenotype on skimmed‐milk plates. hasF encodes a protein sharing 74% identity with the E. coli TolC protein. Anti‐TolC antibodies cross‐reacted with a protein with an apparent molecular weight of 53 kDa in E. coli expressing hasF and in S. marcescens. hasF is unlinked to the has cluster and, unlike the has operon, is not iron regulated. hasF complements some of the tolC phenotypes, including drug‐ and detergent sensitivities and haemolysin secretion but not colicin E1 uptake. This suggests that the various functions of TolC could correspond to distinct domains on the protein.
Value Predispositions, Mass Media, and Attitudes Toward Nanotechnology: The Interplay of Public and Experts

This study examines the factors influencing public and experts’ attitudes toward nanotechnology. Compared with the experts, the public judged nanotechnology as having greater risks and lesser benefits, and indicated less support for federal funding of nanotechnology. Experts used trust in scientists while public used religious beliefs as heuristic cues to make risk judgments. Although deference to scientific authority, science media use, and trust in scientists shaped perceived benefits in both groups, these heuristic cues influenced public perceptions to a larger extent than experts’ perceptions. Experts used relatively less cues to form decision about funding support for nanotechnology as compared with the public.
Target flexibility: an emerging consideration in drug discovery and design.

Department of General and Inorganic Chemistry, UniVersity of Parma, Via G.P. Usberti 17/A 43100, Parma, Italy, National Institute for Biosystems and Biostructures, Rome, Italy, Department of Medicinal Chemistry and Institute for Structural Biology & Drug DiscoVery, Virginia Commonwealth UniVersity, Richmond, Virginia 23298-0540, Department of Pharmaceutics, UniVersity of Parma, Via GP Usberti 27/A, 43100 Parma, Italy, High Performance Systems, CINECA Supercomputing Centre, Casalecchio di Reno, Bologna, Italy, Dulbecco Telethon Institute, Department of Chemistry, UniVersity of Modena and Reggio Emilia, Via Campi 183, 41100 Modena, Italy, Department of Mathematics and Natural Sciences, Pharmaceutical Institute, Christian-Albrechts-UniVersity, Gutenbergstrasse 76, 24118 Kiel, Germany, Departments of Biochemistry & Molecular Biology, Computer Science & Engineering, and Physics & Astronomy, Michigan State UniVersity, East Lansing, Michigan 48824-1319, Department of Molecular Biology, MB-5, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037-1000, Molecular Modeling and Bioinformatics Unit, Institute of Biomedical Research, Scientific Park of Barcelona, Department of Biochemistry and Molecular Biology, UniVersity of Barcelona, Josep Samitier 1-5, Barcelona 08028, Spain, Department of Experimental Medicine, UniVersity of Parma, Via Volturno, 39, 43100, Parma, Italy, Department of Chemical, Food, Pharmaceutical and Pharmacological Sciences, UniVersity of Piemonte Orientale “Amedeo AVogadro”, Via BoVio 6, 28100 NoVara, Italy, Institute of Pharmacy and Food Chemistry, UniVersity of Wurzburg, Am Hubland, D-97074 Wurzburg, Germany