Miracle-dz/UniFAR-FTUs huggingface dataset:
The Feasibility and Acceptability of a Web-Based Alcohol Management Intervention in Community Sports Clubs: A Cross-Sectional Study

Background The implementation of comprehensive alcohol management strategies can reduce excessive alcohol use and reduce the risk of alcohol-related harm at sporting venues. Supporting sports venues to implement alcohol management strategies via the Web may represent an effective and efficient means of reducing harm caused by alcohol in this setting. However, the feasibility and acceptability of such an approach is unknown. Objective This study aimed to identify (1) the current access to and use of the Web and electronic devices by sports clubs; (2) the perceived usefulness, ease of use, and intention to use a Web-based program to support implementation of alcohol management policies in sports clubs; (3) the factors associated with intention to use such a Web-based support program; and (4) the specific features of such a program that sports clubs would find useful. Methods A cross-sectional survey was conducted with club administrators of community football clubs in the state of New South Wales, Australia. Perceived usefulness, ease of use and intention to use a hypothetical Web-based alcohol management support program was assessed using the validated Technology Acceptance Model (TAM) instrument. Associations between intention to use a Web-based program and club characteristics as well as perceived ease of use and usefulness was tested using Fisher’s exact test and represented using relative risk (RR) for high intention to use the program. Results Of the 73 football clubs that were approached to participate in the study, 63 consented to participate and 46 were eligible and completed the survey. All participants reported having access to the Web and 98% reported current use of electronic devices (eg, computers, iPads/tablets, smartphones, laptops, televisions, and smartboards). Mean scores (out of a possible 7) for the TAM constructs were high for intention to use (mean 6.25, SD 0.87), perceived ease of use (mean 6.00, SD 0.99), and perceived usefulness (mean 6.17, SD 0.85). Intention to use the Web-based alcohol management program was significantly associated with perceived ease of use (P=.02, RR 1.4, CI 1.0-2.9), perceived usefulness (P=.03, RR 1.5, CI 1.0-6.8) and club size (P=.02, RR 0.8, CI 0.5-0.9). The most useful features of such a program included the perceived ability to complete program requirements within users’ own time, complete program accreditation assessment and monitoring online, develop tailored action plans, and receive email reminders and prompts to complete action. Conclusions A Web-based alcohol management approach to support sports clubs in the implementation of recommended alcohol management policies appears both feasible and acceptable. Future research should aim to determine if such intended use leads to actual use and club implementation of alcohol management policies.
A Novel Mechanism for Bradykinin Production at Inflammatory Sites

Coprocessing of kininogens by a mixture of human mast cell tryptase and neutrophil elastase was explored as a potential substitute for the kallikrein-dependent pathway for kinin generation during inflammation. Tryptase easily excised bradykinin from the synthetic heptadecapeptide, ISLMKRPPGFSPFRSSR, but was unable to produce significant amounts of kinin by proteolysis of kininogens. However, a mixture of tryptase and elastase released bradykinin from each protein with a yield comparable to that of human plasma kallikrein. Significantly, neither plasma nor tissue kallikrein was able to effectively process N-chlorosuccinimide-oxidized high molecular weight kininogen, an effect attributed to the oxidation of a methionine residue upstream from the N terminus of the kinin domain. In support of these results the model heptadecapetide, ISL(MO)KRPPGFSPFRSSR, was also resistant to hydrolysis by either kallikrein. In contrast, the release of bradykinin from oxidized peptide or protein substrates by the tryptase/elastase mixture was not altered. Because kininogen modification may occur at inflammatory sites, as a result of the oxidative burst of recruited neutrophils and macrophages, these results suggest an alternative pathway for kinin production and the necessity for the novel utilization of two specific proteinases known to be released from these cells during inflammatory episodes.
The Effects of Cognitive Demand upon Relationships between Working Memory and Cognitive Skills

The present study explored the effects of cognitive demand upon relationships between working memory and cognitive skills. Both children and adults were tested on a measure of short-term memory and three measures of counting span, which varied in cognitive demand. When statistically controlling for short-term memory and the speed of counting operations, the more demanding the counting-span task, the closer its relationship with cognitive skills. The results are discussed in terms of practical and theoretical implications for working memory research.
Generalized majority-minority operations are tractable

Let A be a finite set and let /spl phi/ : A/sup k//spl rarr/A with k/spl ges/3 be a k-ary operation on A. We say that /spl phi/ is a generalized majority-minority (GMM) operation if for all a, b /spl isin/ A we have that /spl phi/(x, y,.,y) = /spl phi/(y, x,.,y) =. =/spl phi/(y, y,.,x) = y for all x, y /spl isin/ {a, b} or /spl phi/{x, y,. ,y) = /spl phi/(y, y,.,x) = x for all x, y /spl isin/ {a, b}. Near-unanimity and Mal'tsev operations are particular instances of GMM operations. We prove that every CSP instance where all constraint relations are invariant under a (fixed) GMM operation is solvable in polynomial time. This constitutes one of the largest tractable cases of the CSP.
The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction

Cell-cell fusion between gametes is a defining step during development of eukaryotes, yet we know little about the cellular and molecular mechanisms of the gamete membrane fusion reaction. HAP2 is the sole gamete-specific protein in any system that is broadly conserved and shown by gene disruption to be essential for gamete fusion. The wide evolutionary distribution of HAP2 (also known as GCS1) indicates it was present in the last eukaryotic common ancestor and, therefore, dissecting its molecular properties should provide new insights into fundamental features of fertilization. HAP2 acts at a step after membrane adhesion, presumably directly in the merger of the lipid bilayers. Here, we use the unicellular alga Chlamydomonas to characterize contributions of key regions of HAP2 to protein location and function. We report that mutation of three strongly conserved residues in the ectodomain has no effect on targeting or fusion, although short deletions that include those residues block surface expression and fusion. Furthermore, HAP2 lacking a 237-residue segment of the cytoplasmic region is expressed at the cell surface, but fails to localize at the apical membrane patch specialized for fusion and fails to rescue fusion. Finally, we provide evidence that the ancient HAP2 contained a juxta-membrane, multi-cysteine motif in its cytoplasmic region, and that mutation of a cysteine dyad in this motif preserves protein localization, but substantially impairs HAP2 fusion activity. Thus, the ectodomain of HAP2 is essential for its surface expression, and the cytoplasmic region targets HAP2 to the site of fusion and regulates the fusion reaction. Summary: During Chlamydomonas fertilization, membrane localization and activity of the evolutionarily ancient HAP2 protein depends on cytoplasmic motifs; the ectodomain can not function autonomously.
Word Sense Disambiguation Using Selectional Restriction

Word sense disambiguation (WSD) is still an open research area in natural language processing and computational linguistics. It is from both theoretical and practical point of view. Here, the problem is to find the sense for word in given a context, It is a technique of natural language processing(NLP) ,which requires queries and documents in NLP or texts from Machine Translation (MT). MT is an automated system which involves Marathi, Urdu, Bengali, Punjabi, Hindi, and English etc. Most of the work has been completed in English, and now the focus has been shifted to other languages. The applications of WSD are disambiguation of content in information retrieval (IR), machine translation (MT), speech processing, lexicography, and text processing. In this paper, we have used knowledge based approach along with selectional restriction. It is used to block the formation of component word meanings representation that contains selectional restriction. We have developed a WSD tool using Hindi wordnet. Wordnet is built from co-occurrence, and collocation and it includes synset or synonyms which belong to either noun, verb, adjective, or adverb. In this paper we shall discuss the implementation of our tool and its evaluation.
Information spreading in Online Social Networks: A case study on Twitter network

Information propagation in online social media draw attention in different research domains due to it's influence and importance in public domain. The complexity of this research problem arises due to its huge volume and transient nature. For different user domain data processing, methodologies and derivations of information spreading are quite different in nature. In this paper, we study information propagation in online social network twitter, considering different centrality measures and simulate through random walk. Assuming that the structure of these websites is a kind of scale free network but exhibits the property of small world network, we compare information propagation to sight the most pivotal nodes in the network in aspect of information propagation.
Conversion of tryptophan to indole-3-acetic acid by TRYPTOPHAN AMINOTRANSFERASES OF ARABIDOPSIS and YUCCAs in Arabidopsis

Auxin is an essential hormone, but its biosynthetic routes in plants have not been fully defined. In this paper, we show that the TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) family of amino transferases converts tryptophan to indole-3-pyruvate (IPA) and that the YUCCA (YUC) family of flavin monooxygenases participates in converting IPA to indole-3-acetic acid, the main auxin in plants. Both the YUCs and the TAAs have been shown to play essential roles in auxin biosynthesis, but it has been suggested that they participate in two independent pathways. Here, we show that all of the taa mutant phenotypes, including defects in shade avoidance, root resistance to ethylene and N-1-naphthylphthalamic acid (NPA), are phenocopied by inactivating YUC genes. On the other hand, we show that the taa mutants in several known auxin mutant backgrounds, including pid and npy1, mimic all of the well-characterized developmental defects caused by combining yuc mutants with the auxin mutants. Furthermore, we show that overexpression of YUC1 partially suppresses the shade avoidance defects of taa1 and the sterile phenotypes of the weak but not the strong taa mutants. In addition, we discovered that the auxin overproduction phenotypes of YUC overexpression lines are dependent on active TAA genes. Our genetic data show that YUC and TAA work in the same pathway and that YUC is downstream of TAA. The yuc mutants accumulate IPA, and the taa mutants are partially IPA-deficient, indicating that TAAs are responsible for converting tryptophan to IPA, whereas YUCs play an important role in converting IPA to indole-3-acetic acid.
Diachronous Growth of the Altyn Tagh Mountains: Constraints on Propagation of the Northern Tibetan Margin From (U‐Th)/He Dating

The sinistral Altyn Tagh strike‐slip fault demarcates the northern boundary of the Qinghai‐Tibetan Plateau; however, the significance and timing of activity along the fault are debated. Here new apatite and zircon (U‐Th)/He data are presented, revealing the exhumation history of the Altyn Tagh Mountains from crustal depths of ~2–7 km. Studies of rock samples from the Altun Shan region show that they were located close to the surface during the Early Cretaceous and remained so until about 40 Ma. Samples from the Subei and Dangjin Pass areas, on the other hand, record a period of rapid exhumation at 20 ± 2 Ma, and samples from the Qiemo area record a period of continuous exhumation between ~20 and 12 Ma at a rate of 0.046−0.013+0.007 mm/year, plus a further rapid cooling event at 7–5.5 Ma. Thus, significantly more exhumation has occurred toward the southwestern section of the Altyn Tagh Fault Zone, indicating a decrease in tectonic uplift toward the northeast since the Miocene. During the late Miocene, the Tarim Block began to subduct beneath the northern Tibetan margin, resulting in rapid exhumation of the central section of the Altyn Tagh Fault Zone. In accordance with previous studies, the present results support a model of northward propagation and expansion of the Qinghai‐Tibetan Plateau during the Cenozoic.
Modern media landscape: diversity and fragmentation

The goal of the study is to analyze media diversity and fragmentation as the process of "spreading" audience across multiple smaller channels. Representative samples of Russian TV audiences form the empirical base of analysis. Research suggested that audience fragmentation is characterized by several features. Audience is scattering across numerous thematic sources (each having limited audience). At the same time, there are few high-reach TV channels attracting mass audience. Still, the audiences of small channels are covered by the most popular sources, proportionately to their monthly reach. The research confirms that media diversity and fragmentation correspond to actual social trends. It brings democratization of medialandscape and promoting non-mainstream cultural products. Media diversity and fragmentation mirror information overload and related simplification of media content and change patterns of its understanding. Diversity of media choice does not necessarily bring diversity of media consumption. Commercial imperative focusses media supply and demand on certain media content formats. However, besides fragmentation, there is an ongoing concentration of audience around mass popular channels; this does not support arguments of "demise" of mass media. Media fragmentation follows the trend towards greater diversity and complexity among postmodern societies. It is accompanied also by social disintegration and fragmentation of the public sphere (universal issues fading out of public attention); ever growing content targeting based on digital algorithms and data couples with evident minimization of private sphere for the sake of widespread transparency.
Vibration Perception Threshold as a Measure of Distal Symmetrical Peripheral Neuropathy in Type 1 Diabetes

OBJECTIVE To describe the sensitivity, specificity, positive predictive value, and negative predictive value of vibration perception threshold (VPT) testing in subjects with type 1 diabetes relative to gold standard assessments of peripheral neuropathy. RESEARCH DESIGN AND METHODS VPT was determined in 1,177 adults with type 1 diabetes 13–14 years after participating in a study of intensive (INT) versus conventional (CONV) diabetes treatment. Abnormal VPT was defined by values exceeding 2.5 SD above age-specific normal values. Signs and symptoms of peripheral neuropathy were assessed and electrodiagnostic studies were performed to establish definite clinical neuropathy, abnormal nerve conduction, and confirmed clinical neuropathy (the presence of both definite clinical neuropathy and abnormal nerve conduction). RESULTS Thirty-seven percent of subjects had definite clinical neuropathy, 61% had abnormal nerve conduction, and 30% had confirmed clinical neuropathy. Abnormal VPT was more common among former CONV than among INT subjects (64 vs. 57%, P < 0.05) and was associated with older age. VPT was a sensitive measure of confirmed clinical neuropathy (87%) and of definite clinical neuropathy (80%) and a specific measure of abnormal nerve conduction (62%). Higher VPT cut points improved test sensitivity and lower cut points improved specificity. Areas under the receiver operating characteristic curves ranged from 0.71–0.83 and were higher for older than for younger subjects and highest for those with confirmed clinical neuropathy. CONCLUSIONS VPT was a sensitive measure of peripheral neuropathy. Future researchers may choose to select VPT cut points for defining abnormality based on the population studied and clinical outcome of interest.
Principles of Learning, Implications for Teaching: A Cognitive Neuroscience Perspective.

Cognitive neuroscience aims to improve our understanding of aspects of human learning and performance by combining data acquired with the new brain imaging technologies with data acquired in cognitive psychology paradigms. Both neuroscience and psychology use the philosophical assumptions underpinning the natural sciences, namely the scientific method, whereby hypotheses are proposed and tested using quantitative approaches. The relevance of ‘brain science’ for the classroom has proved controversial with some educators, perhaps because of distrust of the applicability of so-called ‘medical models’ to education. Nevertheless, the brain is the main organ of learning, and so a deeper understanding of the brain would appear highly relevant to education. Modern science is revealing the crucial role of biology in every aspect of human experience and performance. This does not mean that biology determines outcomes. Rather, there is a complex interplay between biology and environments. Improved knowledge about how the brain learns should assist educators in creating optimal learning environments. Neuroscience can also identify ‘biomarkers’ of educational risk, and provide new methodologies to test the effects of educational interventions.
Position-specific trapping of topoisomerase II by benzo[a]pyrene diol epoxide adducts: Implications for interactions with intercalating anticancer agents

DNA topoisomerase II (Top2) is the target of some of the most effective anticancer DNA intercalators. To determine the effect of intercalating ligands at defined positions relative to a known DNA cleavage site for human Top2α, we synthesized oligodeoxynucleotides containing single trans-opened benzo[a]pyrene 7,8-diol 9,10-epoxide (DE) deoxyadenosine (dA) adducts of known absolute configuration, placed at specific positions in a duplex sequence containing staggered Top2 cleavage sites on both strands. Because the orientations of the intercalated hydrocarbon are known from NMR solution structures of duplex oligonucleotides containing these dA adducts, a detailed analysis of the relationship between the position of intercalation and trapping of Top2 is possible. Our findings demonstrate that (i) Top2 cleavage complexes are trapped by intercalation of the hydrocarbon at either of the staggered cleavage sites or immediately adjacent to the base pairs flanking the cleavage sites within the stagger; (ii) both concerted and nonconcerted cleavage by both subunits of a Top2 homodimer were detected depending on the position of the benzo[a]pyrene DE dA adduct; and (iii) intercalation immediately outside of the staggered Top2 cleavage site, and to a lesser extent in the middle of the stagger, prevents Top2 from cleaving DNA at this site, consistent with the effect of some intercalators as suppressors of Top2-mediated DNA cleavage. These results identify specific binding sites for intercalators that result in trapping of Top2. Such poisoning of Top2 by bulky polycyclic aromatic hydrocarbon DE adducts constitutes a potential mechanism for their carcinogenic activity.
Role of a Highly Conserved NH2-Terminal Domain of the Human Parainfluenza Virus Type 3 RNA Polymerase

ABSTRACT The RNA polymerase complex of human parainfluenza virus type 3 (HPIV 3), a member of the family Paramyxoviridae, is composed of two virally encoded polypeptides: a multifunctional large protein (L, 255 kDa) and a phosphoprotein (P, 90 kDa). From extensive deduced amino acid sequence analyses of the cDNA clones of a number of L proteins of nonsegmented negative-strand RNA viruses, a cluster of high-homology sequence segments have been identified within the body of the L proteins. Here, we have focused on the NH2-terminal domain of HPIV 3 L protein that is also highly conserved. Following mutational analyses within this domain, we examined the ability of the mutant L proteins to (i) transcribe an HPIV 3 minireplicon, (ii) transcribe the viral RNA in vitro using the HPIV 3 nucleocapsid RNA template, and (iii) interact with HPIV 3 P protein. Our results demonstrate that the first 15 amino acids of the NH2-terminal domain spanning a highly conserved motif is directly involved in transcription of the genome RNA and in forming a functional complex with the P protein. Substitution of eight nonconserved amino acids within this domain by the corresponding Sendai virus L protein residues yielded mutants with variable transcriptional activities. However, one mutant in which all eight amino acids were replaced with the corresponding residues of Sendai virus L protein failed to both transcribe the minireplicon and interact with HPIV 3 P and the Sendai virus P protein. The possible functional significance of the NH2-terminal domain of paramyxovirus L protein is discussed.
Future changes in extreme storm surges based on mega-ensemble projection using 60-km resolution atmospheric global circulation model

ABSTRACT This study examines long-term ensemble projections for historical and future climate conditions over 5,000 years using an atmospheric global circulation model. The future climate condition is assumed as a constant +4K in the global mean temperature from before the Industrial Revolution (c.a. 1850), and the historical climate condition is perturbed by observed sea surface temperature (SST) error. A set of ensemble experiments assesses the impact of low probability phenomena, such as tropical cyclones and storm surge in comparison with conventional time-slice experiments. Future changes in storm surge will be severe at 15–35°N in the northern hemisphere, especially around the East Asia region. In addition, Future changes in regional storm surges targeting Tokyo and Osaka Bays project 0.3–0.45 m increase of storm surge height with a 100-year return period.
Effect of an extra-virgin olive oil enriched with probiotics or antioxidants on functional dyspepsia: a pilot study.

BACKGROUND While antioxidants and probiotics have been proposed for the treatment of functional dyspepsia, current data are still heterogeneous and studies are poorly designed. Extra-virgin olive oil, a common ingredient of Mediterranean diet, has shown antioxidant properties. AIMS To evaluate the effect of extra-virgin olive oil enriched with antioxidants or probiotics on functional dyspepsia. PATIENTS AND METHODS This study has been designed as a "proof of concept". Extra-virgin olive oil enriched with antioxidants or probiotics was blindly added to the common diet of 8 subjects with functional dyspepsia for 7 days. Dyspeptic symptoms were then evaluated in all patients. RESULTS AND CONCLUSIONS A significant improvement of dyspeptic symptoms was observed in subjects receiving the antioxidant or probiotic enriched oil diet, with a greater effect observed for the latter. Larger studies are now needed to confirm these data.