Miracle-dz/UniFAR-FTUs huggingface dataset:
Use of a Rapid Throughput In Vivo Screen To Investigate Inhibitors of Eukaryotic Topoisomerase II Enzymes

ABSTRACT Topoisomerase II catalyzes the passage of one DNA helix through another via a transient double-stranded break. The essential nature of this enzyme in cell proliferation and its mechanism of action make it an ideal target for cytotoxic agents. Saccharomyces cerevisiae topoisomerase II has been frequently used as a model for testing potential inhibitors of eukaryotic topoisomerase II as antitumor agents. The standard in vivo method of estimating the sensitivity of S. cerevisiae to the antitopoisomerase drugs is via inhibition or kill curves which rely on viable-cell counts and is labor intensive. We present an alternative to this, a high-throughput in vivo screen. This method makes use of a drug-permeable S. cerevisiae strain lacking endogenous topoisomerase II, which is modified to express either human topoisomerase IIα or IIβ or S. cerevisiae topoisomerase II carried on plasmids. Each modified strain expresses a full-length topoisomerase II enzyme, as opposed to the more commonly used temperature-sensitive S. cerevisiae mutant expressing yeast or yeast/human hybrid enzymes. A comparison of this new method with a plating-and-counting method gave similar drug sensitivity results, with increased accuracy and reduced manual input for the new method. The information generated has highlighted the sensitivities of different topoisomerase II enzymes and isoenzymes to several different classes of topoisomerase II inhibitor.
Discourse—sentence interaction in the speech of language-disordered and normal children

The effects of linguistic complexity were examined on children's story discourse. Four groups of 15 subjects each were used: (a) language-disordered; (b) chronologically age matched with normal language abilities; (c) language-age matched; and (d) language-age matched with articulatory errors. The experimental task was sentence production in a story context. Phonological complexity (syllable length of words) was varied. Errors simplified phonological, syntactic and story structures. Older normals exhibited the fewest phonological errors and the greatest mean-length complexity for the sentences produced. The language-disordered children made more phonological errors than the younger normals, although they did not differ from the younger normals in the mean-length sentence complexity. No differences occurred in the number of phonological errors or mean-length sentence complexity between the language-age-matched groups, ones with and without articulatory errors. All four groups exhibited similar story struct.
Control on rate and pathway of anaerobic organic carbon degradation in the seabed

Significance The subsurface seabed is a great anaerobic bioreactor where organic matter deposited from the ocean’s water column is slowly being degraded by microorganisms. It is uncertain how rates of organic matter degradation progress through different geochemical zones, and the deep production of methane and CO2 in the global seabed therefore remains poorly constrained. With highly depth-resolved analyses of geochemistry and microbial activities, we demonstrate that rates of organic matter degradation decrease continuously with sediment age, irrespective of the prevailing redox zonation and associated changes in the degradation pathway. Moreover, our results show that the microbial food chain leading to methane does not proceed directly through the general key intermediate acetate, but rather through an additional, as of yet unidentified, syntrophic step. The degradation of organic matter in the anoxic seabed proceeds through a complex microbial network in which the terminal steps are dominated by oxidation with sulfate or conversion into methane and CO2. The controls on pathway and rate of the degradation process in different geochemical zones remain elusive. Radiotracer techniques were used to perform measurements of sulfate reduction, methanogenesis, and acetate oxidation with unprecedented sensitivity throughout Holocene sediment columns from the Baltic Sea. We found that degradation rates transition continuously from the sulfate to the methane zone, thereby demonstrating that terminal steps do not exert feedback control on upstream hydrolytic and fermentative processes, as previously suspected. Acetate was a key intermediate for carbon mineralization in both zones. However, acetate was not directly converted into methane. Instead, an additional subterminal step converted acetate to CO2 and reducing equivalents, such as H2, which then fed autotrophic reduction of CO2 to methane.
Evaluation of blood culture procedures in a pediatric hospital

To determine optimal clinical laboratory techniques for detecting pediatric bacteremia, we studied 7,768 consecutive blood cultures in a 1-year period. Blood was inoculated into one vented 50-ml bottle of brucella broth with 0.05% sodium polyanetholsulfonate and one unvented 50-ml bottle of Columbia broth with 0.05% sodium polyanetholsulfonate and 0.05% cysteine. Bottles were visually examined for growth on days 1 through 7 and blindly subcultured aerobically and anaerobically on days 1, 2, and 7. There were 724 (9.3%) positive cultures, and 484 (6.2%) were clinically significant. The most frequent isolates from bacteremic patients were Haemophilus influenzae (24%) and Streptococcus pneumoniae (17%). Growth was noted in only one bottle in 25% of clinically significant isolates. Bottles inoculated with greater than or equal to 1 ml of blood became positive earlier than bottles inoculated with less than 1 ml. After 1 day of incubation, 48% of the clinically significant cultures showed growth on visual examination, whereas 85% showed growth on subculture. Only 19% of Haemophilus isolates were detected visually on day 1, whereas 88% were recovered on subculture. By day 7, 3.5% of all isolates (including 18% of pneumococcal isolates and 1% of Haemophilus isolates) could no longer be recovered on subculture. We conclude that a two-bottle blood culture system and blind subculture within 24 h will optimize detection of pediatric bacteremia.
Prospects of performing multiple-country comparison of dairy sires for countries not participating in Interbull international genetic evaluations

International trade with genetic material has lead to the foundation of Interbull, an international organization that performs international evaluations of dairy bulls, enabling countries to obtain breeding values of foreign bulls on their on scale. Not all countries have the possibility to participate in the evaluations with their own national breeding values. The aim of this study was to find an alternative method that does not require national breeding values. Argentina was used as a representative of countries without national breeding values. For this study national breeding values for 909 Argentinean bulls were available; these records were used in a normal Interbull international evaluation and used as reference. The alternative method considers Argentinean bulls as unrecorded, but correlated to bulls in other countries. This method was used with seven different correlation matrices, giving seven alternatives to compare with the reference. Results showed correlation between reference and the alternative methods ranging between 0.926 and 0.973, and loss in genetic gain between 2.82% and 11.9%. This means that this alternative method could be used for countries that do not have national breeding values to submit for international evaluations.
Viewing Responsible Consumption as a Personal Project

Previous research on “responsible consumption” (RC) has focused on the inconsistencies of responsible consumers and has used the term “gap” to refer to these inconsistencies. Observable difficulties, such as a lack of available responsible products and their higher associated costs, have been given as explanations for this gap. A much more complex explanation emerges when RC is seen holistically, a long-term perspective is adopted, when consumers are studied in their daily life, and consumption is seen as a social activity. The argument developed in this paper draws fundamentally on psychology, in particular on the literature on personal projects. By using an inductive methodology (grounded theory), a case is made for treating responsible consumption as one of many personal projects that an individual may undertake. Since consumers have a project network and projects are not always aligned with one another, there is frequently a clash between projects. This clash can be interpersonal (with other projects of the same individual) or intrapersonal (with the projects, beliefs, norms of his/her significant others). The main contribution of this paper is that it puts forward a holistic, dynamic, and socially embedded view of RC which leads to questioning the notion of “gap. ”
Evolution and development of Brain Networks: From Caenorhabditis elegans to Homo sapiens

Neural networks show a progressive increase in complexity during the time course of evolution. From diffuse nerve nets in Cnidaria to modular, hierarchical systems in macaque and humans, there is a gradual shift from simple processes involving a limited amount of tasks and modalities to complex functional and behavioral processing integrating different kinds of information from highly specialized tissue. However, studies in a range of species suggest that fundamental similarities, in spatial and topological features as well as in developmental mechanisms for network formation, are retained across evolution. ‘Small-world’ topology and highly connected regions (hubs) are prevalent across the evolutionary scale, ensuring efficient processing and resilience to internal (e.g. lesions) and external (e.g. environment) changes. Furthermore, in most species, even the establishment of hubs, long-range connections linking distant components, and a modular organization, relies on similar mechanisms. In conclusion, evolutionary divergence leads to greater complexity while following essential developmental constraints.
Emotion-focused therapy for complex trauma: An integrative approach (2nd ed.).

Psychotherapy clients with histories of childhood abuse and complex relational trauma are ubiquitous, and have notoriously high drop-out rates. These clients have been unable to heal past emotional injuries and often have difficulty handling exposure-based therapies, which usually are not designed for attachment related problems. Successful therapy requires helping clients access and explore painful feelings in order to modify maladaptive emotions. Emotion-Focused Therapy for Trauma (EFTT) is the only trauma therapy that is based on an empirically-verified model that identifies steps in the process of resolving past relational issues. In this book, the authors plumb fifteen years of research involving clinical trials, observation and analysis of therapy sessions, as well as their own extensive clinical experience to describe precisely how EFTT works to heal complex trauma. The book is organized into two main sections: Part I describes the EFTT treatment model and the theory behind it, while Part II examines clients' progress through the four phases of treatment, each of which can be revisited in a recursive fashion. The authors focus on the typical progression, beginning with cultivating the therapeutic alliance, through modifying self-concept, resolution of attachment injuries, and termination. Throughout the text, the authors make comparisons with other treatment approaches, and provide clinical examples of different kinds of emotion and emotional processing difficulties. This book will appeal to clinicians and researchers alike and is particularly suitable for use in outpatient trauma clinics and graduate programs that emphasize service and training in empirically-supported treatments.
DISORGANIZATION OF ENDOTHELIUM BY FIBRIN Weimar and Delvos 141 Loading of Fibrin Clots with Antibodies

Deposition of polymerizing fibrin on the vascular endothelium is the final event in intravascular coagulation. Exposure of fibrin clots to confluent monolayers of cultured human endothelial cells for 4 to 24 hours resulted in the disappearance of their normal cobblestone morphology and in the formation of endothelial cell aggregates. The present study was designed to evaluate the conditions and structural requirements of the fibrin clot for the induction of disorganization. Even after harsh treatment with denaturing agents or loading with large amounts of fibrinogen antibodies, polymerized fibrin always induced disorganization of the monolayers. In contrast, soluble fibrin that was kept in solution by either fibrinogen, fragment D-cate, or the tetrapeptide Gly-Pro-Arg-Pro did not cause any alteration of the monolayers. The fibrinogen degradation product D-cate (Mr 94,000) itself had no microscopically detectable influence on the monolayer structure. In the absence of fibrin, the effect of thrombin on endothelial cells was found to be distinct from that induced by fibrin; however, the exposure of pieces of glass coverslips caused alterations in morphology indistinguishable from the fibrin-induced disorganization of the monolayer. Experiments using protein-coated polyester films indicated that the ability of the endothelial cells to attach to the overlying material, independent of its chemical structure, is the prerequisite for the induction of disorganization, but not a defined component of the fibrin molecule. Disorganization of vascular endothelium in vivo might be important for the organization and revascularization of an occluding thrombus. (Arteriosclerosis 6:139-145, March/April 1986)
Biological Principles of Human Attractiveness Perception

Attractiveness has long been deemed as elusive and somewhat mystical phenomena, but researchers in the fields such as experimental psychology and cognitive neuroscience are beginning to unravel the biological basis of human attractiveness perception. The current lecture overviews the findings from multidisciplinary studies on the biological basis of attractiveness perception from human faces and draws some basic principles that define physical attractiveness.
Moderate alcohol consumption and the immune system: A review

Increasing evidence suggests that light to moderate amounts of polyphenol-rich alcoholic beverages like wine or beer could have health benefits. Scientists have long debated the effects of alcohol on immune function, showing on the one hand, that high doses of alcohol consumption can directly suppress a wide range of immune responses, and that alcohol abuse is associated with an increased incidence of a number of infectious diseases. On the other hand, moderate alcohol consumption seems to have a beneficial impact on the immune system compared to alcohol abuse or abstinence. Therefore, the link between alcohol consumption, immune response, as well as infectious and inflammatory processes remains not completely understood. With this in mind, it is important to realise that other factors, unrelated or indirectly related to immune function, like drinking patterns, beverage type, amount of alcohol, or gender differences, will affect the influence that alcohol consumption may have on the immune system. This review summarises published data describing the effects that light to moderate amounts of polyphenol-rich beverages like wine or beer seem to have on immunity in healthy adults.
Dealing with a Troubled Rhodesian Past: Narrative Detachment and Intimacy in Peter Godwin's Mukiwa (1996)

Summary This article argues that one of the challenges white Zimbabwean writers have to deal with in their narratives is a troubled colonial past. In Peter Godwin's Mukiwa, A White Boy in Africa, there is a plain acknowledgement that Rhodesia had problems of legitimacy, which made the treatment of blacks before and during the war unjustified. Godwin's rendition of the past is therefore informed by this recognition, compelling the author to employ narrative strategies which make it possible for him to embrace certain aspects of the past while simultaneously distancing himself from others. This analysis of Godwin's Mukiwa shows how a re-imagined childhood consciousness enables an understanding of the Rhodesian past. Through this narrative strategy, Godwin is supposedly faithful in rendering the past, including its imperfections. Furthermore, the Rhodesian past is depicted as a baneful entity that estranges whites from the Zimbabwean present.
RAR1 Positively Controls Steady State Levels of Barley MLA Resistance Proteins and Enables Sufficient MLA6 Accumulation for Effective Resistance

The polymorphic barley (Hordeum vulgare) Mla locus harbors allelic race-specific resistance (R) genes to the powdery mildew fungus Blumeria graminis f sp hordei. The highly sequence-related MLA proteins contain an N-terminal coiled-coil structure, a central nucleotide binding (NB) site, a Leu-rich repeat (LRR) region, and a C-terminal non-LRR region. Using transgenic barley lines expressing epitope-tagged MLA1 and MLA6 derivatives driven by native regulatory sequences, we show a reversible and salt concentration–dependent distribution of the intracellular MLA proteins in soluble and membrane-associated pools. A posttranscriptional process directs fourfold greater accumulation of MLA1 over MLA6. Unexpectedly, in rar1 mutant plants that are compromised for MLA6 but not MLA1 resistance, the steady state level of both MLA isoforms is reduced. Furthermore, differential steady state levels of MLA1/MLA6 hybrid proteins correlate with their requirement for RAR1; the RAR1-independent hybrid protein accumulates to higher levels and the RAR1-dependent one to lower levels. Interestingly, yeast two-hybrid studies reveal that the LRR domains of RAR1-independent but not RAR1-dependent MLA isoforms interact with SGT1, a RAR1 interacting protein required for the function of many NB-LRR type R proteins. Our findings implicate the existence of a conserved mechanism to reach minimal NB-LRR R protein thresholds that are needed to trigger effective resistance responses.
Challenges in predicting community periodontal index from hospital dental care records

Many studies have been performed in predicting periodontal diseases based on genetic information, dental images or patients habits but few have yet used dental visits records. This paper proposes a methodology based on Random Forest to classify the periodontal disease condition of patients and a way to assess the most important features that lead to a successful classification. We investigate three problematic issues found in dental care records: noise, class imbalance and concept drift and propose solutions to overcome them by respectively detecting and removing noise, under-sampling and only considering recent data. Experiments performed on records from Finnish public hospitals of two cities had good classification results and feature importance was able to detect dentists with poor performance with respect to diagnosis and treatment application.
Continuous high and low temperature induced a decrease of photosynthetic activity and changes in the diurnal fluctuations of organic acids in Opuntia streptacantha

Opuntia plants grow naturally in areas where temperatures are extreme and highly variable in the day during the entire year. These plants survive through different adaptations to respond to adverse environmental conditions. Despite this capability, it is unknown how CAM photosynthetic activity and growth in Opuntia plantlets is affected by constant heat or cold. Therefore, the main objective of this research was to evaluate the short-term effect of high (40°C) and low (4°C) continuous temperatures on the photosynthetic efficiency, the organic acid content (malic acid) and the relative growth rate (RGR) in seven-month-old Opuntia streptacantha plantlets during 5, 10, and 15 days. Chlorophyll fluorescence analysis allowed us to determine that high temperatures negatively impact the photosynthetic efficiency of O. streptacantha plantlets, which exhibited the lowest values of maximum quantum efficiency of the photosystem II (Fv/Fm = 52%, Fv/F0 = 85%), operational quantum yield of PS (ΦPSII = 65%) and relative electron transport rate (rETR = 65%), as well as highest values of basal fluorescence (F0 = 226%) during 15 days of treatment. Similarly, low temperatures decreased Fv/Fm (16%), Fv/F0 (50%), ΦPSII and rETR (16%). High temperatures also decreased nocturnal acidification in approximately 34–50%, whereas low temperatures increased it by 30–36%. Additionally, both continuous temperatures affected drastically diurnal consumption of malic acid, which was related to a significant RGR inhibition, where the specific photosynthetic structure area component was the most affected. Our results allowed determining that, despite the high tolerance to extreme temperatures described for Opuntia plants, young individuals of O. streptacantha suffered photosynthetic impairment that led to the inhibition of their growth. Thus, the main findings reported in this study can help to predict the potential impact of climatic change on the establishment and survival of succulent species of arid and semiarid regions of Mexico.
Spokesmen Election Problem Revisited

The spokesmen election (SE) problem was first proposed in the wave expansion broadcast (WEB) protocol for minimizing broadcast latency in wireless multihop networks. It refers to how to select a set of transmitters from all informed nodes so that the number of uninformed nodes having correct reception is maximal. In this paper, the authors formulate the SE problem into posiform maximization problem, which is a special case of pseudo-Boolean optimization problems. Since maximizing posiform f is equivalent to finding the stability number of f's conflict graph, existing approximate algorithms for computing stability number could be utilized to find a near- optimal solution of SE problem; and new lower bound of the number of uninformed nodes with correct receptions is derived. Numerical results show that our proposed solution outperforms previous spokesmen election algorithm (SEA) in terms of both broadcast latency and broadcast redundancy.
espiratory sinus arrhythmia , shyness , and effortful control in preschool-age hildren ichael

Resting respiratory sinus arrhythmia (RSA) and shyness were examined as predictors of effortful control (EC) in a sample of 101 preschool-age children. Resting RSA was calculated from respiration and heart rate data collected during a neutral film; shyness was measured using parents’, preschool teachers’, and classroom observers’ reports; and EC was measured using four laboratory tasks in addition to question-
Logical Gaps in the Approximate Solutions of the Social Learning Game and an Exact Solution

After the social learning models were proposed, finding solutions to the games becomes a well-defined mathematical question. However, almost all papers on the games and their applications are based on solutions built either upon an ad-hoc argument or a twisted Bayesian analysis of the games. Here, we present logical gaps in those solutions and offer an exact solution of our own. We also introduce a minor extension to the original game so that not only logical differences but also differences in action outcomes among those solutions become visible.
What Is Sexual Pain? A Critique of DSM's Classification of Dyspareunia and Vaginismus

Abstract The Diagnostic and Statistical Manual of Mental Disorders (DSM-III-R) introduced the term “sexual pain disorder” to classify dyspareunia and vaginismus as sexual dysfunctions (American Psychiatric Association, 1987). However, the notion of sexual pain as well as the diagnostic criteria for dyspareunia and vaginismus are questionable both on theoretical and empirical grounds. Recent studies support the view that dyspareunia is better classified as a pain disorder and challenge the validity of the vaginal spasm criterion for vaginismus. Alternative conceptualizations of these conditions are presented.