Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptomic and Translatomic Analyses Reveal Insights into the Signaling Pathways of the Innate Immune Response in the Spleens of SPF Chickens Infected with Avian Reovirus

Avian reovirus (ARV) infection is prevalent in farmed poultry and causes viral arthritis and severe immunosuppression. The spleen plays a very important part in protecting hosts against infectious pathogens. In this research, transcriptome and translatome sequencing technology were combined to investigate the mechanisms of transcriptional and translational regulation in the spleen after ARV infection. On a genome-wide scale, ARV infection can significantly reduce the translation efficiency (TE) of splenic genes. Differentially expressed translational efficiency genes (DTEGs) were identified, including 15 upregulated DTEGs and 396 downregulated DTEGs. These DTEGs were mainly enriched in immune regulation signaling pathways, which indicates that ARV infection reduces the innate immune response in the spleen. In addition, combined analyses revealed that the innate immune response involves the effects of transcriptional and translational regulation. Moreover, we discovered the key gene IL4I1, the most significantly upregulated gene at both the transcriptional and translational levels. Further studies in DF1 cells showed that overexpression of IL4I1 could inhibit the replication of ARV, while inhibiting the expression of endogenous IL4I1 with siRNA promoted the replication of ARV. Overexpression of IL4I1 significantly downregulated the mRNA expression of IFN-β, LGP2, TBK1 and NF-κB; however, the expression of these genes was significantly upregulated after inhibition of IL4I1, suggesting that IL4I1 may be a negative feedback effect of innate immune signaling pathways. In addition, there may be an interaction between IL4I1 and ARV σA protein, and we speculate that the IL4I1 protein plays a regulatory role by interacting with the σA protein. This study not only provides a new perspective on the regulatory mechanisms of the innate immune response after ARV infection but also enriches the knowledge of the host defense mechanisms against ARV invasion and the outcome of ARV evasion of the host’s innate immune response.
An Inverse Kinematic Model for the 3-PRS Compliant Parallel Mechanism With Forward Causative Force

The inverse kinematic model is an essential mathematical tool of the performance analysis and motion control for the parallel mechanism. A diversity of mathematical methods is used for inverse kinematic modelling. However, for compliant parallel mechanism, the direction of the causative force in the model is always disregarded in previous works, which leads to large deviation due to the force sensitivity of the flexure hinge. A straightforward index is proposed to quantitatively evaluate the deviation. A new approach presented in this paper try to overcome this shortcoming, by considering the forward causative force during the inverse kinematic modelling, which makes model realize less deviation and more practical in motion control. Compliant matrix method is adopted to analyze the deformation of the flexure hinge. The effectiveness of the proposed methodology is verified, based on a 3-PRS compliant parallel mechanism, with finite element analysis simulation. The proposed methodology can be employed and extended to a variety of compliant parallel mechanism.
ACT001 improved cardiovascular function in septic mice by inhibiting the production of proinflammatory cytokines and the expression of JAK-STAT signaling pathway

Sepsis is a life-threatening multiple organ dysfunction syndrome (MODS) caused by a microbial infection that leads to high morbidity and mortality worldwide. Sepsis-induced cardiomyopathy (SIC) and coagulopathy promote the progression of adverse outcomes in sepsis. Here, we reported that ACT001, a modified compound of parthenolide, improved the survival of sepsis mice. In this work, we used cecal ligation and puncture (CLP) model to induce SIC. Transthoracic echocardiography and HE staining assays were adopted to evaluate the influence of ACT001 on sepsis-induced cardiac dysfunction. Our results showed that ACT001 significantly improved heart function and reduced SIC. Coagulation accelerates organ damage in sepsis. We found that ACT001 decreased blood clotting in the FeCl3-induced carotid artery thrombosis experiment. ACT001 also reduced the production of neutrophil extracellular traps (NETs). RNA-sequencing of heart tissues revealed that ACT001 significantly downregulated the expression of pro-inflammatory cytokines and the JAK-STAT signaling pathway. These results were confirmed with real-time PCR and ELISA. In summary, we found ACT001 rescued mice from septic shock by protecting the cardiovascular system. This was partially mediated by inhibiting pro-inflammatory cytokine production and down-regulating the JAK-STAT signaling.
A Case of Nonsteroidal Anti-Inflammatory Drug-Induced Anaphylaxis Confirmed by Skin Testing

Nonsteroidal anti-inflammatory drugs (NSAIDs) are collectively a major culprit of drug-induced hypersensitivity. No reliable diagnostic tests, other than a direct challenge, are available for detecting the hypersensitivity. Cross-reactivity among NSAIDs that inhibit cyclooxygenase-1 is common. However, in rare cases, the mechanism underlying hypersensitivity can be understood from the immunological point of view, without involving cross-reactivity of NSAIDs or even with a positive skin test for an NSAID. A 55-year-old woman was referred to the Emergency Department for anaphylaxis. She suffered from generalized hives, chest tightness, and hypotension a few minutes after intramuscular injection of diclofenac. One year ago, she had experienced a similar reaction after intramuscular injection of aceclofenac. Thereafter, she had been taking naproxen as needed to relieve her osteoarthritis pain, without having an adverse reaction. To confirm drug hypersensitivity and to find alternative drugs, provocation tests were performed with acetaminophen, celecoxib, and lysine-aspirin. All tests were negative, and a skin prick test with diclofenac was also negative. However, intradermal injection of 0.05 mL (37.5 mg/mL) diclofenac provoked an anaphylactic shock and resulted in her admission. Here, we report a rare case of single NSAID-induced anaphylaxis, which was only triggered by acetic acid derivatives of NSAIDs, presumably by an immunoglobulin E-mediated reaction.
Efficacy of Repetitive Transcranial Magnetic Stimulation (rTMS) Combined with Psychological Interventions: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

(1) Background: Psychological interventions are effective in alleviating neuropsychiatric symptoms, though results can vary between patients. Repetitive transcranial magnetic stimulation (rTMS) has been proven to improve clinical symptoms and cognition. It remains unclear whether rTMS can augment the efficacy of psychological interventions. (2) Methods: We examined the effects of rTMS combined with psychological interventions on clinical, functional, and cognitive outcomes from randomized controlled trials conducted in healthy and clinical populations. We searched PubMed, EMBASE, Cochrane Library, and PsycINFO databases up to April 2023. (3) Results: Twenty-seven studies were ultimately included. Compared to sham rTMS combined with psychological interventions, active rTMS combined with psychological interventions significantly improved overall clinical symptoms (k = 16, SMD = 0.31, CIs 0.08 to 0.54, p < 0.01). We found that 10 or more sessions of rTMS combined with cognitive behavioural therapy significantly improved clinical outcomes overall (k = 3, SMD = 0.21, CIs 0.05 to 0.36, Z = 2.49, p < 0.01). RTMS combined with cognitive training (CT) significantly improved cognition overall compared to sham rTMS combined with CT (k = 13, SMD = 0.28, CIs 0.15 to 0.42, p < 0.01), with a significant effect on global cognition (k = 11, SMD = 0.45, CIs 0.21 to 0.68, p < 0.01), but not on the other cognitive domains. (4) Conclusion: The current results provide preliminary support for the augmentation effects of active rTMS on clinical and cognitive outcomes across diverse populations. Future clinical trials are required to confirm these augmentation effects for specific psychological interventions in specific clinical populations.
Relationships of phosphorus fractions to organic carbon content in surface soils in mature subtropical forests, Dinghushan, China

Exploring the relationship between the accumulation of soil organic carbon (C) and the form and availability of soil phosphorus (P) is important for improved understanding of soil P availability and its regulation of C storage in forest ecosystems. Here, we investigated the relationships among soil organic C, sequentially extracted P fractions and P sorption index in 32 surface soils (0–0.15 m depth) across eight mature subtropical forests (80–400 years) in Dinghushan, China. Results showed that soil organic P (Po) accounted for 40–63% (mean 54%) of soil total P. Soil organic C was significantly positively correlated with both the content and the percentage of soluble inorganic P (Pi), Al-Po and Fe-Po fractions and the content of the Al-Pi fraction. The content of soil total Po increased significantly with soil organic C, whereas the percentage of soil total Po tended to increase with soil organic C only when soil organic C was low (<30 Mg/ha) but was relatively stable when soil organic C was high (≥30 Mg/ha). Moreover, soil organic C was highly correlated with P sorption index. Our results suggest that accumulation of organic C may increase, rather than decrease, the availability of P in surface soil in mature subtropical forests.
Comparative Study of Small-RNA and Degradome Sequencing Reveals Role of Novel stu-miR8006 in Regulating Root Development in Solanum tuberosum L.

MicroRNAs are a class of endogenous, non-coding, small-RNA molecules with important functions in plant development and stress response processes. Root systems are important because they allow plants to absorb nutrients and water from the soil and are fundamental for anchoring the plant and responding to environmental signals. However, the roles of miRNAs underlying root development remain poorly investigated in potato. In this research, small-RNA sequencing was performed to thoroughly detect underlying miRNAs and their roles in regulating root development between the early root (ER) stage and the mature root (MR) stage of potato roots. A total of 203 known and 137 novel miRNAs were obtained, and 64 differentially expressed miRNAs (DEMs) were identified between the ER and MR stages. The expression patterns of 12 DEMs were also determined via qRT-PCR. In addition, a mixed degradome library was constructed from the ER and the MR stages to identify the targets of the identified miRNAs, and 2400 target genes were verified to be the targets of 131 miRNAs. Based on target annotation, we identified that nine target genes of six DEMs were probably involved in potato root development, and eight targets of six DEMs were validated via 5’-RLM-RACE assays. These targets may participate in root development by regulating cell proliferation, root cultures (PGSC0003DMT400013837), root meristem growth (PGSC0003DMT400079970), root morphogenesis (PGSC0003DMT400040282), post-embryonic root development (PGSC0003DMT400021612), root hair elongation (PGSC0003DMT400034518), cell wall repair (PGSC0003DMT400074930), and auxin polar transport (PGSC0003DMT400079970), and by negatively regulating cell proliferation (PGSC0003DMT400009997) and cell growth (PGSC0003DMT400003464). The qRT-PCR analysis indicated that most miRNAs have opposing expression patterns to their targets. It is widely accepted that potato root development is regulated by miRNAs, among which stu-miR8006-p5-1ss9AT is substantially down-regulated during root development. We show here that the suppression of stu-miR8006-p5-1ss9AT led to an alteration in the potato root architecture and that it targeted auxin induction in the root culture protein 12-encoding gene that is potentially involved in the regulation of root development. In addition, the suppression of stu-miR8006-p5-1ss9AT led to a significant alteration in the potato root architecture. Altogether, our results might provide some useful insights into stu-miR8006-p5-1ss9AT and the crucial role that it plays in potato root development; they could also facilitate the molecular genetic breeding of potato.
Moisture Transport and Other Hydrometeorological Features Associated with the Severe 2000/01 Drought over the Western and Central Canadian Prairies

Abstract The 54-yr (1948–2001) NCEP–NCAR reanalysis data as well as other information were used to study the moisture transport and associated circulation features for the severe 2000/01 drought over the western and central Canadian Prairies. Most of the moisture for precipitation over the region is from the Pacific Ocean in winter (November–March) and from the Gulf of Mexico in summer (May–August). An analysis shows that the zonal moisture transport from the Pacific Ocean into both the North American continent and the western and central Canadian Prairies during the winter of the 2000/01 agricultural year was the least over the entire study period, and there was no significantly enhanced moisture influx from the Gulf of Mexico into the region to compensate. Very low winter precipitation was produced over the western and central Canadian Prairies as a consequence. During the ensuing summer period, moisture transport from the Gulf of Mexico was significantly less than normal and no significantly enhanced m.
Structural and Adsorption Properties of ZIF-8-7 Hybrid Materials Synthesized by Acid Gas-Assisted and De Novo Routes

The tuning of micropore environments in zeolitic imidazolate frameworks (ZIFs) by mixed-linker synthesis has the potential for enabling new molecular separation properties. However, de novo synthesis of mixed-linker (hybrid) ZIFs is often challenging due to the disparate chemical properties of the different linkers. Here, we elucidate the structure and properties of an unconventional ZIF-8-7 hybrid material synthesized via a controlled-acid-gas-assisted degradation and reconstruction (solvent-assisted crystal redemption, SACRed) strategy. Selective insertion of benzimidazole (ZIF-7 linker) into ZIF-8 using SACRed is used as a facile method to generate a ZIF-8-7 hybrid material that is otherwise difficult to synthesize by de novo methods. Detailed crystal structure and textural characterizations clarify the significant differences in the microstructure of the SACRed-derived ZIF-8-7 hybrid material relative to a de novo synthesized hybrid of the same overall linker composition as well as the parent ZIF-8 material. Unary and binary adsorption measurements reveal the tunability of adsorption characteristics as well as the prevalence of nonideal cooperative mixture adsorption effects that lead to large deviations from predictions made with ideal adsorbed solution theory.
Large-Scale Climatic Controls on Lake Baikal Ice Cover.

Abstract Long-term records of winter ice duration, formation, and breakup dates (1869–1996) and maximum thickness (1950–95) on Lake Baikal are analyzed to determine the nature of temporal trends and the relationship with the large-scale atmospheric circulation. There are highly significant trends of decreasing ice duration (and thickness) over the period, associated with later ice formation and earlier breakup dates. These trends are broadly in line with those of winter air temperatures in the region. Variability in Lake Baikal ice formation date, duration, and thickness is significantly related to winter temperatures over a wide area from the Caspian Sea to the Pacific and from northern India to the Kara Sea off the northern coast of Siberia. Thus, Lake Baikal ice cover is a robust indicator of continental-scale winter climate. Correlation and composite analysis of surface and upper-atmospheric fields reveal that interannual variability in ice cover is associated with a tripolar pattern of upper-level ge.
Annular Electromagnetic Generator for Harvesting Ocean Wave Energy

To sustainably power ocean sensors by harvesting ocean wave energy, an annular electromagnetic generator (A-EMG) based on the principle of Faraday electromagnetic induction is proposed in this paper. The specific structure and working principle of the generator are introduced. The distribution of the magnetic field in the coil, the variation in the induced voltage and the influence of the coil parameters on the output were simulated by the COMSOL Multiphysics software version 6.0. At the same time, an experimental platform was built to test the output characteristics of the generator. Through a comparative study of the capacitor’s charging characteristics, the optimal connection mode between the multiple groups of coils of the generator was preliminarily verified. Finally, the six-degree-of-freedom (6-DOF) platform was used to simulate various wave motion parameters, and the feasibility of the generator for supplying power to ocean sensors was verified.
Digital Twin-Assisted Federated Learning Service Provisioning Over Mobile Edge Networks

Federated Learning (FL) offers collaborative machine learning without data exposure, but challenges arise in the mobile edge network (MEC) environment due to limited resources and dynamic conditions. This paper presents a Digital Twin (DT)-assisted FL platform for MEC networks and introduces a novel multi-FL service framework to address resource dynamics and mobile users. We leverage DT models to optimize device scheduling and MEC resource allocation, aiming to maximize utility across FL services. Our work includes heuristic and constant approximation algorithms for offline multi-FL service scenarios and we also investigate an online setting of our solution with dynamic bandwidth and moving client conditions. To adapt to changing network conditions, we utilize historical bandwidth data in DTs and implement a deep reinforcement learning algorithm, Ra_DDPG, for automatic bandwidth allocation. Evaluation results demonstrate a significant 49.8% increase in system utility compared to a benchmark algorithm, showcasing the effectiveness of our approach.
Cyclic Detectors in the Fraction-of-Time Probability Framework

The signal detection problem for cyclostationary signals is addressed within the fraction-of-time probability framework, where statistical functions are constructed starting from a single time series, without introducing the concept of stochastic process. Single-cycle detectors and quadratic-form detectors based on measurements of the Fourier coefficients of the almost-periodically time-variant cumulative distribution and probability density functions are proposed. The adopted fraction-of-time approach provides both methodological and implementation advantages for the proposed detectors. For single-cycle detectors, the decision statistic is a function of the received signal and the threshold is derived using side data under the null hypothesis. For quadratic-form detectors, the decision statistic can be expressed as a function of the received signal without using side data, at the cost of some performance degradation. The threshold can be derived analytically. Performance analysis is carried out using Monte Carlo simulations in severe noise and interference environments, where the proposed detectors provide better performance with respect to the analogous detectors based on second- and higher-order cyclic statistic measurements.
Caracterização petrofísica dos carbonatos da Formação Morro do Chaves, Bacia de Sergipe-Alagoas

A caracterizacao de reservatorios carbonaticos e um trabalho complexo devido a alta heterogeneidade dessas rochas. Com as recentes descobertas dos reservatorios barremianos/aptianos pertencentes a fase rifte da Bacia de Santos, observa-se a importância de novos estudos que auxiliem a interpretacao e caracterizacao destes reservatorios. Com este proposito, foram estudadas sucessoes carbonaticas aflorantes da Formacao Morro do Chaves, Bacia de Sergipe-Alagoas, analogas aos reservatorios da Bacia de Santos. A caracterizacao destas rochas foi realizada atraves do estudo de afloramentos para avaliar aspectos de escala mais ampla e descricao petrografica para identificacao de caracteristicas em micro-escala, visando a identificacao das potenciais facies reservatorio com base nos dados petrofisicos. O espaco poroso foi caracterizado utilizando imagens de tomografia computadorizada de raios-x, determinando a heterogeneidade, anisotropia e volume elementar representativo das amostras. Para a utilizacao dos dados de tomografia, e proposto um novo metodo de segmentacao de imagens tridimensionais baseado na rede neural de Self-Organizing Maps. A partir dos resultados, foram determinados o valor da porosidade absoluta e conectividade do espaco poroso, validando o metodo como uma ferramenta consistente para a analise quantitativa e qualitativa do espaco poroso em rochas heterogeneas. Abstract
Biomaterials for Alveolar Ridge Preservation as a Preoperative Procedure for Implant Treatment: History and Current Evidence

In implant treatment, the reduction and structural changes in the alveolar ridge that occur after tooth extraction limit the length, width, and placement position of the implant body, impair esthetics, and, in some cases, make implant placement difficult. To solve these problems, an alveolar ridge preservation (ARP) technique, which is performed simultaneously with tooth extraction, generally aims to promote bone regeneration and prevent alveolar ridge reduction by filling the extraction socket with bone graft material and then covering it with a barrier membrane to protect against the invasion of epithelial tissue. The extraction socket provides a favorable environment for bone regeneration throughout the healing period because the blood supply is abundant, and it effectively retains the bone graft material by using the remaining bone wall of the socket. In recent years, advances in bioengineering technology have led to the development of graft materials with various biological properties, but there is currently no clear consensus regarding the selection of surgical techniques and materials depending on the condition of the alveolar ridge. This review will provide a comprehensive survey of the evidence accumulated to date on ARP, present many cases according to the clinical situation, and discuss various treatment options.
Impact of Bariatric Surgery on Subtilisin/Kexin Type 9 (PCSK9) Gene Expression and Inflammation in the Adipose Tissue of Obese Diabetic Rats

Bariatric surgery improves dyslipidaemia and reduces body weight, but it remains unclear how bariatric surgery modulates gene expression in fat cells to influence the proprotein convertase subtilisin/kexin type 9 (PCSK-9) and low-density lipoprotein receptor (LDLR) gene expression. The expression of the PCSK9/LDLR/tumor necrosis factor-alpha (TNFα) gene in adipose tissue was measured in two groups of Zucker Diabetic Sprague Dawley (ZDSD) rats after Roux-en-Y gastric bypass (RYGB) surgery or ‘SHAM’ operation. There was lower PCSK9 (p = 0.02) and higher LDLR gene expression (p = 0.02) in adipose tissue in rats after RYGB. Weight change did not correlate with PCSK9 gene expression (r = −0.5, p = 0.08) or TNFα gene expression (r = −0.4, p = 0.1). TNFα gene expression was positively correlated with PCSK9 gene expression (r = 0.7, p = 0.001) but not correlated with LDLR expression (r = −0.3, p = 0.3). Circulating triglyceride levels were lower in RYGB compared to the SHAM group (1.1 (0.8–1.4) vs. 1.5 (1.0–4.2), p = 0.038) mmol/L with no difference in cholesterol levels. LDLR gene expression was increased post-bariatric surgery with the potential to reduce the number of circulating LDL particles. PCSK9 gene expression and TNFα gene expression were positively correlated after RYGB in ZDSD rats, suggesting that the modulation of pro-inflammatory pathways in adipose tissue after RYGB may partly relate to PCSK9 and LDLR gene expression.