Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficient Quiescent Infection of Normal Human Diploid Fibroblasts with Wild-Type Herpes Simplex Virus Type 1

ABSTRACT Quiescent infection of cultured cells with herpes simplex virus type 1 (HSV-1) provides an important, amenable means of studying the molecular mechanics of a nonproductive state that mimics key aspects of in vivo latency. To date, establishing high-multiplicity nonproductive infection of human cells with wild-type HSV-1 has proven challenging. Here, we describe simple culture conditions that established a cell state in normal human diploid fibroblasts that supported efficient quiescent infection using wild-type virus and exhibited many important properties of the in vivo latent state. Despite the efficient production of immediate early (IE) proteins ICP4 and ICP22, the latter remained unprocessed, and viral late gene products were only transiently and inefficiently produced. This low level of virus activity in cultures was rapidly suppressed as the nonproductive state was established. Entry into quiescence was associated with inefficient production of the viral trans-activating protein ICP0, and the accumulation of enlarged nuclear PML structures normally dispersed during productive infection. Lytic replication was rapidly and efficiently restored by exogenous expression of HSV-1 ICP0. These findings are in agreement with previous models in which quiescence was established with HSV mutants disrupted in their expression of IE gene products that included ICP0 and, importantly, provide a means to study cellular mechanisms that repress wild-type viral functions to prevent productive replication. We discuss this model in relation to existing systems and its potential as a simple tool to study the molecular mechanisms of quiescent infection in human cells using wild-type HSV-1.
CONTRASTIVE LEARNER CORPUS ANALYSIS OF EPISTEMIC MODALITY AND INTERLANGUAGE PRAGMATIC COMPETENCE IN L2 WRITING

This paper reports on a preliminary study on second language (L2) learners’ interlanguage pragmatic (ILP) development in academic written discourse by examining how epistemic modality is used by non-native speaker (NNS) writers compared with NS writers in both native speaker (NS) and NNS corpus data. This study also investigates how NNS writers gradually develop interlanguage pragmatic competence in academic writing across L2 proficiency levels. The study of epistemic modality in academic writing not only provides us valuable insights on understanding the concepts of intercultural competence and L2 acquisitional pragmatics, but it also contributes to ILP and Second Language Acquisition (SLA) research and the teaching of L2 pragmatics. The findings of the study thus point to a need for cultural-sensitive curricula and explicit pragmatic instructions in writing classrooms, with an aim to emphasize the importance of acquisitional process of intercultural pragmatic competence.
The Research of the Credit Evaluation Based on Projection Pursuit-Fuzzy Clustering System Model

This paper presents an approach based on Projection Pursuit and fuzzy rule extraction combining new hybrid method of classification system. This method is the first to use projection pursuit technology to deal with training set of sample dimensionality reduction and in accordance with the sample classification. According to the results of the classification and the best value projection, using trapezoid distribution method extraction fuzzy rules, producing three types of fuzzy membership function. Finally, in accordance with Fuzzy nearness value to determined the sample under the credit level. The results of experiment showed this method of classification accuracy than the traditional credit evaluation techniques have markedly improved, the reliability is better, has a good application value.
Audio-to-score Alignment at the Note Level for Orchestral Recordings

In this paper we propose an offline method for refining audio-to-score alignment at the note level in the context of orchestral recordings. State-of-the-art score alignment systems estimate note onsets with a low time resolution, and without detecting note offsets. For applications such as score-informed source separation we need a precise alignment at note level. Thus, we propose a novel method that refines alignment by determining the note onsets and offsets in complex orchestral mixtures by combining audio and image processing techniques. First, we introduce a note-wise pitch salience function that weighs the harmonic contribution according to the notes present in the score. Second, we perform image binarization and blob detection based on connectivity rules. Then, we pick the best combination of blobs, using dynamic programming. We finally obtain onset and offset times from the boundaries of the most salient blob. We evaluate our method on a dataset of Bach chorales, showing that the proposed approach can accurately estimate note onsets and offsets.
Expression of prolactin receptors in normal, benign, and malignant breast tissue: an immunohistological study.

AIMS Prolactin plays an important role in the proliferation and differentiation of normal breast epithelium, and possibly in the development of breast carcinoma. The effects of prolactin are mediated by its receptor; thus, alteration in the expression of this receptor could be important in studying the biology of breast cancer. This investigation was aimed at comparing the expression of prolactin receptors in normal, benign, and malignant breast tissue. MATERIAL/METHODS The expression of prolactin receptors was studied in paraffin wax embedded sections of 102 breast biopsies (93 female and nine male), using the monoclonal antibody B6.2, and the avidin-biotin immunoperoxidase technique. Six biopsies were normal, 34 had benign lesions, and 62 were malignant. RESULTS In normal cases, prolactin receptor positivity was seen only on the luminal borders of the epithelial cells lining ducts and acini. In most benign lesions, variable degrees of luminal and cytoplasmic staining were seen. Cells showing apocrine metaplasia and florid regular ductal epithelial hyperplasia were mostly negative. In malignant cases, the staining pattern was mostly cytoplasmic and heterogeneous. Forty one of the 59 carcinomas in women showed a degree of positivity involving 10-100% of the tumour cells. A significant direct correlation was found between prolactin receptor and oestrogen receptor staining when only cases that scored more than 100/300 for the latter receptor, using the H scoring system, were considered (p = 0.0207). No correlation was found between prolactin receptors and progesterone receptors, patient's age, tumour size, tumour grade, or axillary lymph node status. CONCLUSIONS Prolactin receptors seem to be expressed at different cellular sites in normal, benign, and malignant breast epithelial cells. The receptor is expressed in more than two thirds of female breast carcinomas, suggesting that it may play a role in the pathogenesis of the disease. The positivity is correlated with moderate and strong staining for oestrogen receptors in tissue sections, but not with other prognostic factors.
Antitumor activity and long-term fate of chimeric antigen receptor-positive T cells in patients with neuroblastoma.

We generated MHC-independent chimeric antigen receptors (CARs) directed to the GD2 antigen expressed by neuroblastoma tumor cells and treated patients with this disease. Two distinguishable forms of this CAR were expressed in EBV-specific cytotoxic T lymphocytes (EBV-CTLs) and activated T cells (ATCs). We have previously shown that EBV-CTLs expressing GD2-CARs (CAR-CTLs) circulated at higher levels than GD2-CAR ATCs (CAR-ATCs) early after infusion, but by 6 weeks, both subsets became low or undetectable. We now report the long-term clinical and immunologic consequences of infusions in 19 patients with high-risk neuroblastoma: 8 in remission at infusion and 11 with active disease. Three of 11 patients with active disease achieved complete remission, and persistence of either CAR-ATCs or CAR-CTLs beyond 6 weeks was associated with superior clinical outcome. We observed persistence for up to 192 weeks for CAR-ATCs and 96 weeks for CAR-CTLs, and duration of persistence was highly concordant with the percentage of CD4(+) cells and central memory cells (CD45RO(+)CD62L(+)) in the infused product. In conclusion, GD2-CAR T cells can induce complete tumor responses in patients with active neuroblastoma; these CAR T cells may have extended, low-level persistence in patients, and such persistence was associated with longer survival. This study is registered at www.clinialtrials. gov as #NCT00085930.
Proceedings of the First International Workshop on Dynamic and Adaptive Hypertext : Generic Frameworks , Approaches and Techniques ( DAH ’ 09 )

Nowadays, Web applications have become more sophisticated and interactive, and many of them also offer personalization and adaptation as their features. The mechanisms for these features are typically built on top of specific adaptive engine that has its own engine-specific adaptation language that can be very different from other engines. However, our observation leads to the fact that the main objective of these adaptive engines is the same, that is, to create adaptive navigation structure. In this paper, we present GAL (Generic Adaptation Language) that specifies the basic adaptive navigation structure in an engine independent way. Since GAL is generic, it can be compiled to difference specific adaptive engine and be used in combination with different authoring environments.
Conservation in human-modi fi ed landscapes

In the previous two chapters, we learn about the importance and difficulties of prioritizing areas for conservation (Chapter 11), and the management of endangered species in these habitats (Chapter 12). In this chapter, we discuss the challenges of conserving biodiversity in degraded and modified landscapes with a focus on the tropical terrestrial biome, which is undergoing rapid deforestation and habitat degradation (Chapter 4) and contains an untold diversity of rare and endemic species that are in urgent need of conservation attention. We first highlight the extent to which human activities have modified natural ecosystems, and how these changes are fundamental in defining ongoing conservation efforts around the world. We then outline opportunities for conserving biodiversity within the dominant types of human land-use, including logged forests, agroforestry systems, monoculture plantations, agricultural lands, urban areas, and regenerating land. We also highlight the highly dynamic nature of modified landscapes and the need to recognize important human development benefits that can be derived from conservation action in these areas.
Refinement of Adaptivity by Reflection

Adaptivity is a system's ability to respond flexibly to dynamically changing needs. Adaptivity to human needs, wishes and desires-even to those that might be unconsciously present-is a particularly ambitious task. A digital system which is expected to behave adaptively has to learn about the needs and desires to which it shall adapt. Advanced adaptivity requires learning on the system's side. Under realistic application conditions, information about a human user available to a computerized system usually is highly incomplete. Therefore, the system's learning process is unavoidably error-prone and the knowledge on which the system's adaptive behavior has to rely is hypothetical by nature. Adaptive system behavior is improved by the system's ability to reflect on the reliability of its current hypothetical knowledge.
Interest on Reserves and Daylight Credit

Payment of interest on banks' reserves has long been an important subject of discussion in monetary policy forums. Recent legislation that allows the U.S. Federal Reserve to pay interest on reserves starting in 2011 has made these issues especially timely. By studying a simple model of the demand for reserves by banks, one can better understand the alternative schemes available for implementing the payment of interest on reserves. This article's primary focus is to investigate the possible consequences that paying interest on overnight bank balances can have on the use of daylight credit by banks. By paying interest on reserves and supplying a large amount of reserves, the central bank can limit the use of daylight credit by depository institutions.
A New Statistically Uncorrelated Discriminant Plane

We present a study on the statistically uncorrelated optimal discriminant plane in this paper. A new concise formula for finding the statistically uncorrelated optimal discriminant plane has been developed, which overcomes the shortcoming of Jin's statistically uncorrelated optimal discriminant plane. The numerical experiments conducted on IRIS data verify the correctness of the above statements
An Acetamide Derivative as a Camptothecin Sensitizer for Human Non-Small-Cell Lung Cancer Cells through Increased Oxidative Stress and JNK Activation

In recent years, combination chemotherapy is a primary strategy for treating lung cancer; however, the issues of antagonism and side effects still limit its applications. The development of chemosensitizer aims to sensitize chemoresistant cancer cells to anticancer drugs and therefore improve the efficacy of chemotherapy. In this study, we examined whether N-[2-(morpholin-4-yl)phenyl]-2-{8-oxatricyclo[7.4. 0.0,2,7]trideca-1(9),2(7),3,5,10,12-hexaen-4-yloxy}acetamide (NPOA), an acetamide derivative, sensitizes human non-small-cell lung cancer (NSCLC) H1299 cells towards camptothecin- (CPT-) induced apoptosis effects. Our results demonstrate that the combination of CPT and NPOA enhances anti-lung-cancer effect. The cytometer-based Annexin V/propidium iodide (PI) staining showed that CPT and NPOA cotreatment causes an increased population of apoptotic cells compared to CPT treatment alone. Moreover, Western blotting assay showed an enhancement of Bax expression and caspase cascade leading to cell death of H1299 cells. Besides, CPT and NPOA cotreatment-mediated disruption of mitochondrial membrane potential (MMP) in H1299 cells may function through increasing the activation of the stressed-associated c-Jun N-terminal kinase (JNK). These results showed that NPOA treatment sensitizes H1299 cells towards CPT-induced accumulation of cell cycle S phase and mitochondrial-mediated apoptosis through regulating endogenous ROS and JNK activation. Accordingly, NPOA could be a candidate chemosensitizer of CPT derivative agents such as irinotecan or topotecan in the future.
Terminal Mobility in Broadband Networks

The convergence of telecommunications, computing and content industries has been one of the major trends during the recent years. The convergence has lead t o the creation of a wide range of multimedia services which are available in digital for m through broadband networks. In this paper we consider implications of this development to the connection control of m bile multimedia services when terminal mobility is present.
Deadlock avoidance policies for automated manufacturing cells

The problem of deadlock resolution arising in manufacturing environments organized according to the typical process-layout has only recently been undertaken by the scientific community. Furthermore, deadlock avoidance methodologies, which seem to be the most appropriate deadlock-handling strategies in this particular context, unfortunately, in the general case, suffer from increased computational complexity results in heuristic solutions and/or reduced performance. This paper proposes an analytical framework for designing scalable and yet provably correct deadlock avoidance policies for a subclass of resource allocation systems (RAS), which is appropriate for the study of the deadlock problem in the context of the automated manufacturing cell (AMC). The framework is used to prove the correctness of the resource upstream neighborhood (RUN) policy, developed in the authors' research program.
General Discussion on Dimming Control Method Used for Discharge Lamp

In order to reduce the energy, the dimming control is used in the lighting system. There are many different control methods in the fluorescent lamp, after comparing these methods, a research scheme of dimming control used in the HID ballast is proposed. In addition, the lamp model, dimming range and stability analysis are also analyzed in this paper.
Brownian motion with dry friction: Fokker–Planck approach

We solve a Langevin equation, first studied by de Gennes, in which there is a solid–solid or dry friction force acting on a Brownian particle in addition to the viscous friction usually considered in the study of Brownian motion. We obtain both the time-dependent propagator of this equation and the velocity correlation function by solving the associated time-dependent Fokker–Planck equation. Exact results are found for the case where only dry friction acts on the particle. For the case where both dry and viscous friction forces are present, series representations of the propagator and correlation function are obtained in terms of parabolic cylinder functions. Similar series representations are also obtained for the case where an external constant force is added to the Langevin equation.
Workplace Bullying and Mental Health: A Meta-Analysis on Cross-Sectional and Longitudinal Data

Background A growing body of research has confirmed that workplace bullying is a source of distress and poor mental health. Here we summarize the cross-sectional and longitudinal literature on these associations. Methods Systematic review and meta-analyses on the relation between workplace bullying and mental health. Results The cross-sectional data (65 effect sizes, N = 115.783) showed positive associations between workplace bullying and symptoms of depression (r = .28, 95% CI = .23–.34), anxiety (r = .34, 95% CI = .29–.40) and stress-related psychological complaints (r = .37, 95% CI = .30–.44). Pooling the literature that investigated longitudinal relationships (26 effect sizes, N = 54.450) showed that workplace bullying was related to mental health complaints over time (r = 0.21, 95% CI = 0.13–0.21). Interestingly, baseline mental health problems were associated with subsequent exposure to workplace bullying (r = 0.18, 95% CI = 0.10–0.27; 11 effect sizes, N = 27.028). Limitations All data were self-reported, raising the possibility of reporting- and response set bias. Conclusions Workplace bullying is consistently, and in a bi-directional manner, associated with reduced mental health. This may call for intervention strategies against bullying at work.
Learning treasures within an unauthorized migrant workers’ community

This study was designed to explore the learning mechanism and its treasures within an undocumented migrant workers’ voluntary community in Korea. Based on a qualitative case study, major findings suggest that various modes of learning mechanisms acted as crucial foundations of the community’s diverse learning paths and participants’ social engagement. Four mechanisms steered the community towards a sustainable learning environment: constructiveness, communicativeness, collective autonomy and practicality. Through these, unauthorized migrants were able to have their “voices” heard in the wider society. These learning treasures have implications for both theory and practice, and for empowering underprivileged groups. They could also widen the horizon of understanding human learning in an informal learning context.
Correction: miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria

The authors would like to correct the Author Contributions. The correct contributions are: Conceptualized the study: Y.J.L T.Ry M.A T.R. Contributed materials and processed the small RNA reads: T.Ry. Performed the miRNA predictions and produced figures: Y.J.L. Wrote the first draft of the manuscript: Y.J.L. M.A. Wrote the final version of the manuscript: Y.J.L T.Ry M.A T.R.
The Radion Mode in Consistent Brane-World Reductions

We construct consistent brane-world Kaluza-Klein reductions involving the radion mode that measures the separation of the domain-wall branes. In these new examples, we can obtain matter supermultiplets coupled to supergravity on the brane, starting from pure gauged supergravity in the higher dimension. This contrasts with previously-known examples of consistent brane-world reductions involving the radion, where either pure supergravity reduced to pure supergravity, or else supergravity plus matter reduced to supergravity plus matter. As well as considering supersymmetric reductions, we also show that there exist broader classes of consistent reductions of bosonic systems. These include examples where the lower-dimensional theory has non-abelian Yang-Mills fields and yet the scalar sector has a potential that admits Minkowski spacetime as a solution. Combined with a sphere reduction to obtain the starting point for the brane-world reduction, this provides a Kaluza-Klein mechanism for obtaining non-abelian gauge symmetries from the geometry of the reduction, whilst still permitting a Minkowski vacuum in the lower dimension.
Enabling wireless communications with state-of-the-art RF CMOS and SiGe BiCMOS technologies

The choice of technology for today's mixed-signal/RF system-on-chip (SOC) designs has been driven by the performance enhancements and cost advantage derived from scaled CMOS technologies. This paper discusses the performance improvements of RF transistors resulting from technology downscaling. Comparisons between scaled RF CMOS and SiGe BiCMOS technologies to highlight the benefits of employing SiGe HBT devices in certain applications are made. Other technology enablements discussed include accurate, scalable models and statistical models to address the need for design flexibility and robust manufacturing. Thereafter the introduction of high Q inductors, high density capacitors and varactors as basic passive components for RF circuits are discussed. Analog requirements such as mismatch, temperature linearity and voltage linearity are also discussed.
Capture and Replay of User Executions to Improve Software Quality

Because today’s software is increasingly dynamic and runs in heterogeneous environments, it is difficult to assess software systems outside the actual context in which they execute. Therefore, the results of in-house quality-assurance activities, such as testing, are often not representative of the way the software will behave in the field. To help addressing this problem, we present a technique for capturing and replaying partial executions of deployed software. Given an application, the technique allows for selecting a subsystem, capturing the dynamic interactions between such subsystem and the rest of the application, and replaying the recorded interactions on the subsystem in isolation. Our technique is designed to be efficient, in that we only capture relevant dynamic information and disregard all data that, although flowing through the boundary of the subsystem of interest, do not affect the execution. After describing our technique, we discuss several possible applications of the approach, including generation of test cases from users’ executions and post-mortem dynamic analysis. In the paper, we also present scarpe, a prototype tool that implements our technique for Java programs. Finally, we present an empirical evaluation of the technique performed using scarpe. The results of the studies show the feasibility of the approach.