Miracle-dz/UniFAR-FTUs huggingface dataset:
Micellar Liquid Chromatographic Determination of Carbaryl and 1-Naphthol in Water, Soil, and Vegetables

A liquid chromatographic procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite 1-naphthol, using a C18 column (250 mm × 4.6 mm) with a micellar mobile phase and fluorescence detection at maximum excitation/emission wavelengths of 225/333 nm, respectively. In the optimization step, surfactants sodium dodecyl sulphate (SDS), Brij-35 and N-cetylpyridinium chloride monohydrate, and organic solvents propanol, butanol, and pentanol were considered. The selected mobile phase was 0.15 M SDS-6% (v/v)-pentanol-0.01 M NaH2PO4 buffered at pH 3. Validation studies, according to the ICH Tripartite Guideline, included linearity (r > 0.999), limit of detection (5 and 18 ng mL−1, for carbaryl and 1-naphthol, resp.), and limit of quantification (15 and 50 ng mL−1, for carbaryl and 1-naphthol, resp.), with intra- and interday precisions below 1%, and robustness parameters below 3%. The results show that the procedure was adequate for the routine analysis of these two compounds in water, soil, and vegetables samples.
Differences in Synaptic Dysfunction Between rTg4510 and APP/PS1 Mouse Models of Alzheimer’s Disease

Genetically modified mice have provided insights into the progression and pathology of Alzheimer’s disease (AD). Here, we have examined two mouse models of AD: the rTg4510 mouse, which overexpresses mutant human Tau gene, and the APP/PS1 mouse, which overexpresses mutant human genes for amyloid precursor protein and presenilin 1. Both models exhibit deficits in hippocampal function, but comparative analyses of these deficits are sparse. We used extracellular field potential recordings in hippocampal slices to study basal synaptic transmission (BST), paired-pulse facilitation (PPF), and long-term potentiation (LTP) at the Schaffer collateral-CA1 pyramidal cell synapses in both models. We found that 6-7, but not 2-3-month-old rTg4510 mice exhibited reduced pre-synaptic activation (fiber volley (FV) amplitude, ∼50%) and field excitatory post-synaptic potential (fEPSP) slope (∼40%) compared to wild-type controls. In contrast to previous reports, BST, when controlled for FV amplitude, was not altered in rTg4510. APP/PS1 mice (2-3 mo and 8-10 mo) had unchanged FV amplitude compared to wild-type controls, while fEPSP slope was reduced by ∼34% in older mice, indicating a deficit in BST. PPF was unchanged in 8–10-month-old APP/PS1 mice, but was reduced in 6-7-month-old rTg4510 mice. LTP was reduced only in older rTg4510 and APP/PS1 mice. Our data suggest that BST deficits appear earlier in APP/PS1 than in rTg4510, which exhibited no BST deficits at the ages tested. However, FV and synaptic plasticity deficits developed earlier in rTg4510. These findings highlight fundamental differences in the progression of synaptic pathology in two genetically distinct models of AD.
Oncologic and obstetrical outcomes with fertility-sparing treatment of cervical cancer: a systematic review and meta-analysis

OBJECTIVE The objectives of this study were to evaluate the rates of recurrence, survival and pregnancy, and characterize pregnancy outcomes of early-stage cervical cancer(eCC) treated with fertility-sparing methods such as cervical conization (CON) and radical trachelectomy(RT) with or without pelvic lymphadenectomy. STUDY DESIGN This was a meta-analysis of observational studies analyzed by a random-effects model and a meta-regression to assess heterogeneity. RESULTS Sixty observational studies encompassing 2,854 patients were included; 17 of which evaluated CON and 43 RT. Three hundred and seventy-five patients were included in the CON group: 176(46.9%) stage IA1 and 167(44.5%) stage IB1. In the RT group, 2479 cases were included: 143(6.0%) stage IA1, 299(12.1%) stage IA2, 1987(79.9%) stage IB1. CON was performed in 347(92.5%) cases, resulting in a recurrence rate of 0.4%(95%CI: 0.0%-1.4%), a death rate of 0%(0%-0%), a pregnancy rate of 36.1%(26.4%-46.2%), a spontaneous abortion rate of 14.8%(9.3%-21.2%) and a preterm delivery rate of 6.8%(1.5%-15.5%). For the RT group, 2273(91.7%) underwent successful surgeries with a recurrence rate of 2.3%(1.3%-3.4%),a death rate of 0.7%(0.3%-1.1%), a pregnancy rate of 20.5%(16.8%-24.5%), a spontaneous abortion rate of 24.0%(18.8%-29.6%) and a preterm delivery rate of 26.6%(19.6%-34.2%). From a subgroup analysis, the recurrence rates for stage IA tumors treated with CON and RT were 0.4%(0.0%-1.9%) and 0.7%(0.0%-2.3%), respectively; and for stage IB were 0.6%(0.0%-2.7%) and 2.3%(0.9%-4.1%). CONCLUSION Fertility-sparing treatment including CON or RT for eCC is feasible and carefully selected women can preserve fertility and achieve pregnancy resulting in live births. CON seems to result in better pregnancy outcomes than RT with similar rates of recurrence and mortality.
drVM: a new tool for efficient genome assembly of known eukaryotic viruses from metagenomes

Abstract Background: Virus discovery using high-throughput next-generation sequencing has become more commonplace. However, although analysis of deep next-generation sequencing data allows us to identity potential pathogens, the entire analytical procedure requires competency in the bioinformatics domain, which includes implementing proper software packages and preparing prerequisite databases. Simple and user-friendly bioinformatics pipelines are urgently required to obtain complete viral genome sequences from metagenomic data. Results: This manuscript presents a pipeline, drVM (detect and reconstruct known viral genomes from metagenomes), for rapid viral read identification, genus-level read partition, read normalization, de novo assembly, sequence annotation, and coverage profiling. The first two procedures and sequence annotation rely on known viral genomes as a reference database. drVM was validated via the analysis of over 300 sequencing runs generated by Illumina and Ion Torrent platforms to provide complete viral genome assemblies for a variety of virus types including DNA viruses, RNA viruses, and retroviruses. drVM is available for free download at: https://sourceforge.net/projects/sb2nhri/files/drVM/ and is also assembled as a Docker container, an Amazon machine image, and a virtual machine to facilitate seamless deployment. Conclusions: drVM was compared with other viral detection tools to demonstrate its merits in terms of viral genome completeness and reduced computation time. This substantiates the platform's potential to produce prompt and accurate viral genome sequences from clinical samples.
TXNDC5 contributes to rheumatoid arthritis by down‐regulating IGFBP1 expression

The thioredoxin domain‐containing 5 (TXNDC5) gene is associated with susceptibility to rheumatoid arthritis (RA) and exhibits increased expression in the synovial tissues. TXNDC5 is also associated strongly with diabetes, a metabolic disease characterized by interrupted insulin signalling. This study investigated whether TXNDC5 contributes to RA via the insulin signalling pathway. In this study, RA synovial fibroblast‐like cells (RASFs) transfected with an anti‐TXNDC5 small interfering RNA (siRNA) were analysed with an insulin signaling pathway RT2 profiler polymerase chain reaction (PCR) array and an insulin resistance RT2 profiler PCR array. The PCR arrays detected significantly increased expression of insulin‐like growth factor binding protein 1 (IGFBP1) in RASFs with suppressed TXNDC5 expression. The result was verified using real‐time PCR and Western blot analyses. Significantly elevated IGFBP1 expression and decreased interleukin (IL)‐6 secretion were also detected in culture medium of transfected RASFs. Furthermore, decreased IGFBP1 mRNA and protein expression levels were detected in RA synovial tissues. Additionally, significantly increased apoptosis and decreased cell proliferation and cell migration were observed in RASFs transfected with the anti‐TXNDC5 siRNA, whereas transfection with the anti‐IGFBP1 siRNA or a mixture of the anti‐IGFBP1 and anti‐TXNDC5 siRNAs restored normal cell proliferation, migration and IL‐6 level in RASFs. Insulin‐like growth factor (IGF) has potent prosurvival and anti‐apoptotic functions, and IGFBP1 can suppress IGF activity. Based on the results of the present study, we suggest that TXNDC5 contributes to abnormal RASF proliferation, migration and IL‐6 production by inhibiting IGFBP1 expression.
Strong MHD helical turbulence and the nonlinear dynamo effect

To understand the turbulent generation of large-scale magnetic fields and to advance beyond purely kinematic approaches to the dynamo effect like that introduced by Steenbeck, Krause & Radler (1966)’ a new nonlinear theory is developed for three-dimensional, homogeneous, isotropic, incompressible MHD turbulence with helicity, i.e. not statistically invariant under plane reflexions. For this, techniques introduced for ordinary turbulence in recent years by Kraichnan (1971 a)’ Orszag (1970, 1976) and others are generalized to MHD; in particular we make use of the eddy-damped quasi-normal Markovian approximation. The resulting closed equations for the evolution of the kinetic and magnetic energy and helicity spectra are studied both theoretically and numerically in situations with high Reynolds number and unit magnetic Prandtl number. Interactions between widely separated scales are much more important than for non-magnetic turbulence. Large-scale magnetic energy brings to equipartition small-scale kinetic and magnetic excitation (energy or helicity) by the ‘Alfvén effect’; the small-scale ‘residual’ helicity, which is the difference between a purely kinetic and a purely magnetic helical term, induces growth of large-scale magnetic energy and helicity by the ‘helicity effect’. In the absence of helicity an inertial range occurs with a cascade of energy to small scales; to lowest order it is a −3/2 power law with equipartition of kinetic and magnetic energy spectra as in Kraichnan (1965) but there are −2 corrections (and possibly higher ones) leading to a slight excess of magnetic energy. When kinetic energy is continuously injected, an initial seed of magnetic field will grow to approximate equipartition, at least in the small scales. If in addition kinetic helicity is injected, an inverse cascade of magnetic helicity is obtained leading to the appearance of magnetic energy and helicity in ever-increasing scales (in fact, limited by the size of the system). This inverse cascade, predicted by Frisch et al. (1975), results from a competition between the helicity and Alféh effects and yields an inertial range with approximately — 1 and — 2 power laws for magnetic energy and helicity. When kinetic helicity is injected at the scale linj and the rate $\tilde{\epsilon}^V$ (per unit mass), the time of build-up of magnetic energy with scale L [Gt ] linj is $t \approx L(|\tilde{\epsilon}^V|l^2_{\rm inj})^{-1/3}. $
Reexamining the Nonlinear Moisture‐Precipitation Relationship Over the Tropical Oceans

Bretherton et al. (2004) used the Special Sensor Microwave Imager (SSM/I) version 5 product to derive an exponential curve that describes the relationship between precipitation and column relative humidity (CRH) over the tropical oceans. The curve, which features a precipitation pickup at a CRH of about 0.75 and a rapid increase of precipitation with CRH after the pickup, has been widely used in the studies of the tropical atmosphere. This study reexamines the moisture‐precipitation relationship by using the version 7 SSM/I data, in which several biases in the previous version are corrected, and evaluates the relationship in the Coupled Model Intercomparison Project phase 5 (CMIP5) models. In the revised exponential curve derived using the updated satellite data, the precipitation pickup occurs at a higher CRH (~0.8), and precipitation increases more slowly with CRH than in the previous curve. In most CMIP5 models, the precipitation pickup is too early due to the common model bias of overestimated (underestimated) precipitation in the dry (wet) regime.
EFFECTS OF FAT REPLACERS AND STABILIZERS ON RHEOLOGICAL, PHYSICOCHEMICAL AND SENSORY PROPERTIES OF REDUCED-FAT ICE CREAM

Current interests towards lowering fat content in food products and producing healthier and safer foods, have convinced ice cream manufacturers to substitute milk fat in ice cream with either carbohydrate or protein based fat replacers. In the present work, reduced-fat ice cream (5% fat) was produced using milk protein concentrate (65%) and inulin as fat replacers (0, 2 and 4%) as well as two types of commercial stabilizeremulsifier blends (Stab-IC80 and Stab-6924) at levels of 0.3 and 0.4%. Rheological, physicochemical and sensory properties of ice cream mix and final ice cream were evaluated. All the mixes were pseudoplastic fluids with apparent viscosity values decreasing with increasing shear rate. Mix viscosity and consistency coefficient increased while flow behaviour index decreasing by use of MPC (Milk Protein Concentrate) and inulin and as well with an increase in either type of stabilizer level. Ice cream hardness was not affected by type and amount of fat replacer and stabilizer but overrun values decreased with increasing MPC, inulin and stabilizers’ level in the product formulation. In general, samples containing Stab-IC80 had greater values of overrun and melting resistance than samples with Stab-6924. According to panel test results, the highest score for sensory evaluation was given to sample containing 2% inulin.
Minimally invasive heart valve surgery: influence on coagulation and inflammatory response.

OBJECTIVES Minimally invasive valve surgery (MIVS) is associated with improved clinical outcome but longer cardiopulmonary bypass (CPB) time. The aim of the present study is to compare inflammatory and coagulation parameters in patients undergoing minimally invasive or standard valve surgery. METHODS A prospective non-randomized study was performed enrolling 79 patients undergoing mitral (20 right mini-thoracotomy and 18 standard sternotomy) and aortic valve (20 mini-sternotomy and 21 standard sternotomy) procedures. Blood samples were collected perioperatively to measure prothrombin fragment 1.2 (PF1.2, thrombin generation), plasmin antiplasmin complex (PAP, fibrinolysis), interleukin-6 (IL-6, inflammation). Plasma free haemoglobin (f-Hb) was assessed to evaluate haemolysis. RESULTS Patients in the minimally invasive group were younger and had less comorbidities CPB and cross-clamp times were comparable considering both aortic and mitral procedures, but longer in the mini-thoracotomy group. IL-6 and PAP were reduced in the minimally invasive group, particularly 2 h after CPB (respectively 102 ± 114 vs 34 ± 49 pg/ml, P < 0.001 and 2137 ± 1046 vs 1207 ± 675 ng/ml, P < 0.001), PF1.2 was also reduced during and after the operation (688 ± 514 vs 571 ± 470, P = 0.02; 1600 ± 1185 vs 1042 ± 548, P < 0.001; 1487 ± 676 vs 1042 ± 541). Despite the use of vacuum-assisted active venous drainage (VAVD) f-Hb was significantly reduced in the minimally invasive group. The other routine biomarkers such as C-reactive protein, fibrinogen and cTnI were also reduced in the minimally invasive group. CONCLUSIONS In a selected cohort of patients MIVS is associated to reduced inflammatory reaction and coagulopathy, supporting the clinical evidence of reduced postoperative bleeding and lower transfusion rate. Our data offer further suggestion supporting the adoption of minimally invasive approaches.
Prediction of adverse perinatal outcome by cerebroplacental ratio adjusted for estimated fetal weight

To evaluate the relationship between cerebroplacental ratio (CPR) and estimated fetal weight (EFW) in low‐ and high‐risk singleton pregnancies. Furthermore, we evaluated the role of CPR in the prediction of adverse perinatal outcome and whether CPR measurements adjusted for EFW improve its predictive value.
Reducing Execution Time of Distributed SELECT Query in Heterogeneous Distributed Database using Genetic Algorithm

Centralized unit that coordinates different types of schema running on multiple sites is getting importance now-a-days. Heterogeneous Distributed Database System (HDDS) is the collection of multiple different databases management systems running on multiple systems that are linked together. Query processing is complicated in such cases. In this work we concentrate on utilizing Genetic Algorithm for finding optimized query execution plan for distributed SELECT queries. Selecting the right set of plans for queries using Genetic Algorithm which minimizes the total execution time is the major goal of this work. Replication of schema is used in this work which gives multiple solutions for retrieval of the data. We used Chromosome for specifying plan for query. Chromosome structures consist of combination of data site and join order. Aglet, Mobile agent, is used to connect all the database servers with centralized server. By implementing this, we get the optimized plan for the select query.
A Primer on Physical-Layer Network Coding

The concept of physical-layer network coding (PNC) was proposed in 2006 for application in wireless networks. Since then it has developed into a subfield of communications and networking with a wide following. This book is a primer on PNC. It is the outcome of a set of lecture notes for a course for beginning graduate students at The Chinese University of Hong Kong. The target audience is expected to have some prior background knowledge in communication theory and wireless communications, but not working knowledge at the research level. Indeed, a goal of this book/course is to allow the reader to gain a deeper appreciation of the various nuances of wireless communications and networking by focusing on problems arising from the study of PNC. Specifically, we introduce the tools and techniques needed to solve problems in PNC, and many of these tools and techniques are drawn from the more general disciplines of signal processing, communications, and networking: PNC is used as a pivot to learn about the fundamentals of signal processing techniques and wireless communications in general. We feel that such a problem-centric approach will give the reader a more in-depth understanding of these disciplines and allow him/her to see first-hand how the techniques of these disciplines can be applied to solve real research problems. As a primer, this book does not cover many advanced materials related to PNC. PNC is an active research field and many new results will no doubt be forthcoming in the near future. We believe that this book will provide a good contextual framework for the interpretation of these advanced results should the reader decide to probe further into the field of PNC.
Off-Treatment Hepatitis B Virus (HBV) DNA Levels and the Prediction of Relapse After Discontinuation of Nucleos(t)ide Analogue Therapy in Patients With Chronic Hepatitis B: A Prospective Stop Study

Background The optimal management remains unknown after nucleos(t)ide analogue (NA) discontinuation in patients with chronic hepatitis B (CHB). This prospective study investigated the role of off-treatment viral kinetics in predicting relapse after discontinuation of NA therapy. Methods A total of 82 noncirrhotic Asian patients with CHB who discontinued NA therapy according to international guidelines were prospectively followed. Patients with a hepatitis B virus (HBV) DNA level of >2000 IU/mL and an alanine aminotransferase (ALT) level of >2 times the upper limit of normal (clinical relapse) were retreated. Results Sixty patients were HBV envelope antigen (HBeAg) positive at the start of treatment, and 22 were HBeAg negative. Clinical relapse developed in 28 patients (2-year rates, 31% among HBeAg-positive patients and 53% among HBeAg-negative patients). Age of ≤35 years (hazard ratio [HR], 0.37; P = .026) and end-of-treatment HBsAg level of ≤200 IU/mL (HR, 0.39; P = .078) were independently associated with lower relapse rates. A high risk of biochemical relapse (defined as an ALT level of >2 times the upper limit of normal) was observed if the HBV DNA level was >200000 IU/mL when the level was initially elevated, compared with HBV DNA levels of >2000 to ≤200000 IU/mL (HR, 8.42; P < .001). The risk of biochemical relapse was also high in patients with persistent elevation in the HBV DNA level (confirmed to be >2000 IU/mL within 3 months), compared with the group with transient elevation (HR, 6.87; P < .001). Conclusions After NA discontinuation, a lower relapse rate was observed in younger patients and in those with low end-of-treatment HBsAg levels. The level and persistence of off-treatment elevated HBV DNA levels were useful in the prediction of a subsequent biochemical relapse and may thus be used to guide off-treatment management.
On the sharp upper bound related to the weak
Muckenhoupt–Wheeden conjecture

We construct an example showing that the upper bound $[w]_{A_1}\log({\rm{e}}+[w]_{A_1})$ for the $L^1(w)\to L^{1,\infty}(w)$ norm of the Hilbert transform cannot be improved in general.
Editor’s Highlight: Transgenic Zebrafish Reporter Lines as Alternative In Vivo Organ Toxicity Models

Organ toxicity, particularly liver toxicity, remains one of the major reasons for the termination of drug candidates in the development pipeline as well as withdrawal or restrictions of marketed drugs. A screening-amenable alternative in vivo model such as zebrafish would, therefore, find immediate application in the early prediction of unacceptable organ toxicity. To identify highly upregulated genes as biomarkers of toxic responses in the zebrafish model, a set of well-characterized reference drugs that cause drug-induced liver injury (DILI) in the clinic were applied to zebrafish larvae and adults. Transcriptome microarray analysis was performed on whole larvae or dissected adult livers. Integration of data sets from different drug treatments at different stages identified common upregulated detoxification pathways. Within these were candidate biomarkers which recurred in multiple treatments. We prioritized 4 highly upregulated genes encoding enzymes acting in distinct phases of the drug metabolism pathway. Through promoter isolation and fosmid recombineering, eGFP reporter transgenic zebrafish lines were generated and evaluated for their response to DILI drugs. Three of the 4 generated reporter lines showed a dose and time-dependent induction in endodermal organs to reference drugs and an expanded drug set. In conclusion, through integrated transcriptomics and transgenic approaches, we have developed parallel independent zebrafish in vivo screening platforms able to predict organ toxicities of preclinical drugs.