Miracle-dz/UniFAR-FTUs huggingface dataset:
Investigating tendon mechanobiology and the potential of high frequency low magnitude loads for tendon repair and remodelling using an novel in vitro loading system

Tendon injuries are ubiquitous in the sporting and occupational environment. Clinically they present a challenge to Orthopaedic surgeons as they account for up to half of all sports injuries and almost half of reported work related ailments. The capacity for tendons to heal subsequent to injury is restricted due to their poor blood supply. Moreover, healed tendon tissue may be inferior to the intact tendon, having diminished biochemical and biomechanical properties and this brings about an ever increasing need for optimized treatment methods for tendon repair. Mechanobiology is concerned with how mechanical forces influence physiological and pathological aspects of the living tissue. However, the complex and poorly controlled loading environment in living organisms prevent the establishment of direct relationships between mechanical stimuli and tissue response. By developing a novel in vitro loading system (IVLS), the work in this thesis investigates the potential of a new and exciting biophysical loading intervention, High Frequency Low Magnitude (HFLM) mechanical loading, for stimulation of tendon repair and remodelling. Following a pre-defined stimulation period, healthy rat tail tendon fascicles (RTTFs) were evaluated for tissue viability and metabolism, Glycosaminoglycan (GAG) content, collagen arrangement and tangent modulus, using staining and biochemical assays, together with microscopy techniques, and mechanical testing. HFLM mechanically loaded tendons showed a trend for a higher tangent modulus than fresh tissue, and significantly higher modulus than unloaded. Further, when varying mechanical loading parameters of frequencies and dosages over clinically relevant ranges, a frequency dependent response was observed with increased tangent modulus and GAG content with increasing frequency. An association between high tendon crimp pattern and elevated tendon modulus as a result of HFLM mechanical loading was also demonstrated. Concomitantly, an injury model was developed to evaluate the effects of in vitro static, low frequency cyclic and HFLM mechanical loading conditions on the biochemical and biomechanical properties of in vitro damaged tendons. HFLM mechanically loaded damaged tendons again demonstrated significantly higher modulus and metabolism than unloaded tissue, although these were reduced below those of fresh damaged tissue. The findings in this thesis together with the newly developed IVLS reveal the potential for an exciting and unique biophysical therapeutic loading intervention for treatment of tendon injuries, and provide a scientific platform for further investigation of the effects of HFLM mechanical loads, potentially leading to an application within the clinic for enhanced connective tissue repair and remodelling.
Fcµ Receptor Promotes the Survival and Activation of Marginal Zone B Cells and Protects Mice against Bacterial Sepsis

The marginal zone B cells (MZB) are located at the interface between the circulation and lymphoid tissue and as a gatekeeper play important roles in both innate and adaptive immune responses. We have previously found that MZB are significantly reduced in mice deficient in the IgM Fc receptor (FcμR) but how FcμR regulates the development and function of MZB remains unknown. In this study, we found that both marginal zone precursor (MZP) and MZB were decreased in FcμR−/− mice. The reduction of MZP and MZB was not due to impaired proliferation of these cells but rather due to their increased death. Further analysis revealed that FcμR−/− MZB had reduced tonic BCR signal, as evidenced by their decreased levels of phosphorylated SYK and AKT relative to WT MZB. MZB in FcμR−/− mice responded poorly to LPS in vivo when compared with MZB in WT mice. Consistent with the reduced proportion of MZB and their impaired response to LPS, antibody production against the type 1 T-independent Ag, NP-LPS, was significantly reduced in FcμR−/− mice. Moreover, FcμR−/− mice were highly susceptible to Citrobacter rodentium-induced sepsis. These results reveal a critical role for FcμR in the survival and activation of MZB and in protection against acute bacterial infection.
Brain–machine interfaces for controlling lower-limb powered robotic systems

Objective. Lower-limb, powered robotics systems such as exoskeletons and orthoses have emerged as novel robotic interventions to assist or rehabilitate people with walking disabilities. These devices are generally controlled by certain physical maneuvers, for example pressing buttons or shifting body weight. Although effective, these control schemes are not what humans naturally use. The usability and clinical relevance of these robotics systems could be further enhanced by brain–machine interfaces (BMIs). A number of preliminary studies have been published on this topic, but a systematic understanding of the experimental design, tasks, and performance of BMI-exoskeleton systems for restoration of gait is lacking. Approach. To address this gap, we applied standard systematic review methodology for a literature search in PubMed and EMBASE databases and identified 11 studies involving BMI-robotics systems. The devices, user population, input and output of the BMIs and robot systems respectively, neural features, decoders, denoising techniques, and system performance were reviewed and compared. Main results. Results showed BMIs classifying walk versus stand tasks are the most common. The results also indicate that electroencephalography (EEG) is the only recording method for humans. Performance was not clearly presented in most of the studies. Several challenges were summarized, including EEG denoising, safety, responsiveness and others. Significance. We conclude that lower-body powered exoskeletons with automated gait intention detection based on BMIs open new possibilities in the assistance and rehabilitation fields, although the current performance, clinical benefits and several key challenging issues indicate that additional research and development is required to deploy these systems in the clinic and at home. Moreover, rigorous EEG denoising techniques, suitable performance metrics, consistent trial reporting, and more clinical trials are needed to advance the field.
A comparison of the immunological effects of propofol and isoflurane for maintenance of anesthesia in healthy dogs

Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clinical blood hematology and immunological profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy adult beagles were included in this study, randomly assigned to the propofol anesthesia group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups, the number of lymphocytes in peripheral blood decreased after 2 hr of anesthesia (2 hr), but group P showed significantly less decrease than group I. For T-lymphocyte subsets examined by flowcytometry, the ratio of CD3+, CD4+ and CD8+ lymphocytes in the peripheral blood mononuclear cell (PBMC) of group P at 2 hr also exhibited a high level compared to group I. Moreover, for mRNA expression of cytokines measured by real-time PCR, the IL2 (pro-inflammatory cytokine) of group P showed no decrease like group I. The IL10 (anti-inflammatory cytokine) of group P also showed no increase like group I, while both cytokines maintained nearly the same level until 2 hr. These results suggest that, compared to propofol, isoflurane had more strongly immuno-suppression caused by anesthesia, and propofol itself might have some immuno-protective effects. Thus, TIVA with propofol might benefit immunological support in the perioperative period of dogs.
Paraffin Control More Cost-Effective

Paraffin control by the thermal and mechanical means that have predominated in the past have done so relatively independent of their cost effectiveness or long term adverse effects. Economic conditions in the industry now require more attention and expertise be applied to solving paraffin problems. Tests and procedures now known allow more efficient use of paraffin control dollars.
New developments in an old strategy: heterologous vector primes and envelope protein boosts in HIV vaccine design

ABSTRACT Prime/boost vaccination strategies for HIV/SIV vaccine development have been used since the early 1990s and have become an established method for eliciting cell and antibody mediated immunity. Here we focus on induction of protective antibodies, both broadly neutralizing and non-neutralizing, with the viral envelope being the key target antigen. Prime/boost approaches are complicated by the diversity of autologous and heterologous priming vectors, and by various forms of envelope booster immunogens, many still in development as structural studies aim to design stable constructs with exposure of critical epitopes for protective antibody elicitation. This review discusses individual vaccine components, reviews recent prime/boost strategies and their outcomes, and highlights complicating factors arising as greater knowledge concerning induction of adaptive, protective immunity is acquired.
Investigation of Commercially Pure Titanium Structure during Accumulation and Release of Hydrogen by Means of Positron Lifetime and Electrical Resistivity Measurements

In this work the goal was to study the behavior of titanium-hydrogen system with hydrogen concentration of 1 wt. % at high temperature vacuum annealing by means of positron lifetime (PL) spectroscopy and electrical resistivity measurements. The range of hydrogen concentrations under investigation requires the γ phase formation. The study is related with the X-ray diffraction (XRD) and thermal desorption spectra (TDS) analysis. The registered positron lifetime spectra were analyzed by multiexponential decomposition into two components. Correlation of changes of the parameters of the PL spectrum and the electrical resistivity is revealed in this paper.
The Feature Selectivity of the Phase of the Local Field Potential in the Primary Visual Cortex

Phase is an important feature of the local field potential (LFP) and plays a significant role in transmission and processing information in visual system. In this paper, the LFP of Long Evans rats primary visual cortex is recorded by the microelectrode array through the visual stimuli of the checkerboard and different orientation gratings. Then, a multi-mode phase extraction model based on the firing spikes was built. We found that neurons selective orientation information using the third intrinsic mode functions of local field potential during firing spikes.
Predictors of revascularization method and long-term outcome of percutaneous coronary intervention or repeat coronary bypass surgery in patients with multivessel coronary disease and previous coronary bypass surgery.

AIMS The optimal revascularization strategy in patients with symptomatic multivessel coronary artery disease (CAD) and previous coronary artery bypass grafting (CABG) remains unknown. METHODS AND RESULTS We evaluated all patients with previous CABG undergoing isolated, non-emergency multivessel revascularization between 1 January 1995 and 31 December 2000. The analysis concentrated on the independent predictors of the revascularization method, as well as on long-term mortality and its predictors, after calculating a propensity score for the method of revascularization. There were 2191 patients (1487 with reoperation and 704 with percutaneous coronary intervention, PCI) in the study. The most important factors in choosing reoperation were presence of more diseased or occluded grafts, previous infarction, lower ejection fraction (EF), longer interval from first CABG, and more total occlusions of native arteries, as well as absence of a patent mammary graft. The distribution of the propensity score was skewed towards the two extremes. At 5 years, the unadjusted cumulative survival was 79.5% for CABG and 75.3% for PCI, P=0.008. After adjustment for the propensity score for PCI vs. CABG, PCI was associated with a hazard ratio of 1.47 (0.94-2.28), P=0.09. The most powerful predictors of mortality were higher age and lower EF. CONCLUSION The choice of the revascularization method in patients with previous CABG is dictated mostly by anatomical considerations and less by clinical characteristics. In contrast, clinical characteristics predominantly affect long-term outcome, whereas the method of revascularization has a limited effect. A randomized clinical trial addressing this important segment of the population with ischaemic heart disease is warranted.
Reconfigurable trusted computing in hardware

Trusted Computing (TC) is an emerging technology towards building trustworthy computing platforms. The TrustedComputing Group (TCG) has proposed several specifications to implement TC functionalities by extensions to common computing platforms, particularly the underlying hardware with a Trusted Platform Module (TPM). However, actual TPMs are mostly available for workstations and servers nowadays and rather for specific domainapplications and not primarily for embedded systems. Further, the TPM specifications are becoming monolithic andmore complex while the applications demand a scalable and flexible usage of TPM functionalities. In this paper we propose a reconfigurable (hardware) architecture with TC functionalities where we focus on TPMsas proposed by the TCG specifically designed for embedded platforms. Our approach allows for (i) an efficient andscalable design and update of TPM functionalities, in particular for hardware-based crypto engines and accelerators, (ii) establishing a minimal trusted computing base in hardware, (iii) including the TPM as well as its functionalities into the chain of trust that enables to bind sensitive data to the underlying reconfigurable hardware, and (iv) designing a manufacturer independent TPM. We discuss possible implementations based on current FPGAs and point out the associated challenges, in particular with respect to protection of the internal TPM state since it must not be subject to manipulation, replay, and cloning
Maximum-A-Posteriori Signal Recovery with Prior Information: Applications to Compressive Sensing

This paper studies the asymptotic performance of maximum-a-posteriori estimation in the presence of prior information. The problem arises in several applications such as recovery of signals with non-uniform sparsity pattern from underdetermined measurements. With prior information, the maximum-a-posteriori estimator might have asymmetric penalty. We consider a generic form of this estimator and study its performance via the replica method. Our analyses demonstrate an asymmetric form of the decoupling property in the large-system limit. Employing our results, we further investigate the performance of weighted zero-norm minimization for recovery of a non-uniform sparse signal. Our investigations illustrate that for a given distortion, the minimum number of required measurements can be significantly reduced by choosing weighting coefficients optimally.
Robust hybridization of nanostructured buried integrated optical waveguide systems with on-chip fluid handling for chemical analysis

We report a method that enables the combination of integrated optical systems with on-chip fluid handling for chemical analysis, without compromising the functionality of either part. Utilizing a selective isotropic plasma etch of a sacrificial chromium etch-stop layer, fluidic connections can be made down to buried nanostructured optical sensing elements in a controlled manner. We describe the fabrication method and present an example application to a label-free assay of protein concentration based on optical slot-waveguide micro-ring resonator refractive index sensors.
A systematic review of the literature exploring the interplay between prostate cancer and type two diabetes mellitus

Prostate cancer (PCa) and type two diabetes mellitus (T2DM) are both increasing prevalent conditions and often occur concurrently. However, the relationship between the two is more complex than just two prevalent conditions co-existing. This review systematically explores the literature around the interplay between the two conditions. It covers the impact of pre-existing T2DM on PCa incidence, grade and stage, as well as exploring the impact of T2DM on PCa outcomes and mortality and the interaction between T2DM and PCa treatments.
Renal denervation in comparison with intensified pharmacotherapy in true resistant hypertension: 2-year outcomes of randomized PRAGUE-15 study

Objectives: The randomized, multicentre study compared the efficacy of renal denervation (RDN) versus spironolactone addition in patients with true resistant hypertension. We present the 24-month data. Methods: A total of 106 patients with true resistant hypertension were enrolled in this study: 52 patients were randomized to RDN and 54 patients to the spironolactone addition, with baseline SBP of 159 ± 17 and 155 ± 17 mmHg and average number of drugs 5.1 and 5.4, respectively. Two-year data are available in 86 patients. Spironolactone addition, as crossover after 1 year, was performed in 23 patients after RDN, and spironolactone addition followed by RDN was performed in five patients. Results: Similar and comparable reduction of 24-h SBP after RDN or spironolactone addition after randomization was observed, 9.1 mmHg (P = 0.001) and 10.9 mmHg (P = 0.001), respectively. Similar decrease of office blood pressure (BP) was observed, 17.7 mmHg (P < 0.001) versus 14.1 mmHg (P < 0.001), whereas the number of antihypertensive drugs did not differ significantly between groups. Crossover analysis showed nonsignificantly better efficacy of spironolactone addition in 24-h SBP and office SBP reduction than RDN (3.7 mmHg, P = 0.27 and 4.6 mmHg, P = 0.28 in favour of spironolactone addition, respectively). Meanwhile, the number of antihypertensive drugs was significantly increased after spironolactone addition (+0.7, P = 0.001). Conclusion: In the settings of true resistant hypertension, spironolactone addition (if tolerated) seems to be of better efficacy than RDN in BP reduction over a period of 24 months. However, by contrast to the 12-month results, BP changes were not significantly greater.
Collision Detection Method Using Self Interference Cancelation for Random Access Multiuser MIMO

This paper proposes an interference detection method for multiuser-multiple input multiple output (MU-MIMO) transmission, which utilizes periodical preamble signals in the frequency domain and the concept of full-duplex transmission when assuming idle antennas at the access point (AP) in MU-MIMO. In the propose method, collision detection (CD) of MU-MIMO is achieved by utilizing asynchronous MU-MIMO called random access MU-MIMO. In random access MU-MIMO, several antennas that are not used for the transmission exist, due to asynchronous MU-MIMO. Hence, idle antennas at the AP can receive preamble signals while the transmit antennas at the AP transmit the preamble signals: this procedure is regarded as full-duplex transmission, which cancels the self-interference between AP antennas. The interference can be detected by subtracting the short preamble signal, which is multiplied by the estimated channel response using the received signal after the FFT processing. Moreover, we utilize dual polarization to reduce the mutual coupling between transmit and receive antennas at the AP. Through a computer simulation, it is shown that the proposed method can successfully detect collision from other user terminals (UTs) with OFDM signals when the interfering power from the interfering user terminal (IT) is greater than the noise power. In addition, the interfering power from IT at the AP and the desired user terminal (DT) is measured in an actual indoor environment, and the possibility of using the proposed method at the AP is discussed by using the measurement results.
Power-of-d-Choices with Memory: Fluid Limit and Optimality

In multiserver distributed queueing systems, the access of stochastically arriving jobs to resources is often regulated by a dispatcher, also known as a load balancer. A fundamental problem consists in designing a load-balancing algorithm that minimizes the delays experienced by jobs. During the last two decades, the power-of-d-choice algorithm, based on the idea of dispatching each job to the least loaded server out of d servers randomly sampled at the arrival of the job itself, has emerged as a breakthrough in the foundations of this area because of its versatility and appealing asymptotic properties. In this paper, we consider the power-of-d-choice algorithm with the addition of a local memory that keeps track of the latest observations collected over time on the sampled servers. Then, each job is sent to a server with the lowest observation. We show that this algorithm is asymptotically optimal in the sense that the load balancer can always assign each job to an idle server in the large-system limit. This holds true if and only if the system load λ is less than [Formula: see text]. If this condition is not satisfied, we show that queue lengths are bounded by [Formula: see text]. This is in contrast with the classic version of the power-of-d-choice algorithm, in which, at the fluid scale, a strictly positive proportion of servers containing [Formula: see text] jobs exists for all [Formula: see text] in equilibrium. Our results quantify and highlight the importance of using memory as a means to enhance performance in randomized load balancing.
Gallbladder Carcinoma Cells in Cerebrospinal Fluid as the First Manifestation of a Tumor

BACKGROUND Meningeal carcinomatosis (MC) rarely occurs as the first evidence of a tumor. In such cases cytology of the cerebrospinal fluid is crucial to the diagnosis. The most frequent primary MCs are lung and breast cancers. MC from a gallbladder carcinoma is uncommon. CASE A 58-year-old woman presented with paroxysmal headaches, seizures and coma. Analysis of the cerebrospinal fluid revealed carcinoma cells and a low protein concentration. Only postmortem examination discovered gallbladder adenocarcinoma to be the source of the tumor cells. CONCLUSION A case with the onset of MC secondary to rare mucinous adenocarcinoma of the gallbladder is presented. Cytology of the cerebrospinal fluid was the only examination that uncovered malignancy. Nine similar cases were found in the literature. Low cerebrospinal fluid protein seems to be of diagnostic value.
A Review of Chinese Herbal Medicine for the Treatment of Chronic Heart Failure

Heart failure is one of the major causes of mortality worldwide and it is the end stage of sev-eral cardiovascular diseases. Traditional Chinese medicine has been used in the management of heart failure for a long time. Only until recently, well-designed clinical trials have been put into practice to study the efficacies of Chinese herbs. Extensive studies have also been carried out to explore the under-lying mechanisms of pharmaceutical actions of Chinese herbs. In this study, we will summarize the frequently used Chinese herbs, formulae and patent Chinese drugs in treating patients with heart failure and review published clinical evaluations of Chinese herbs in treating cardiovascular diseases. The mechanisms by which Chinese herbs exert cardio-protective effects will also be reviewed. In the end, we will point out the limitations of current studies and challenges facing modernization of traditional Chi-nese medicine.