Miracle-dz/UniFAR-FTUs huggingface dataset:
On authentication of cell lines

Work recently published by several of the co-authors of the original RGC-5 paper indicates that the cell line may have never existed outside of the originating laboratory [20]. Cryopreserved RGC-5 passages, some as early as the second passage (P2), were obtained from the originating laboratory. These cells were found to be genetically identical to 661W cells, an immortalized mouse photoreceptor cell line [21] that was in use in the originating laboratory at the time that the RGC-5 cell line was being developed [20]. It is likely that from P2 on, all RGC-5 clones were instead 661W cells [20]. We commend the authors and Investigative Ophthalmology and Vision Science for providing this correction.
Effects of additives on the microstructure and dielectric properties of Ba_2Ti_9O_20 microwave ceramic

Preparation of dense and phase-pure Ba_2Ti_9O_20 is generally difficult to achieve using a solid-state reaction, due to the presence of several thermodynamically stable compounds in the vicinity of the desired composition. This work investigated the effects of various additives (TiO_2, MnO, and ZrO_2) on the densification, microstructural evolution, phase stability, and dielectric properties of Ba_2Ti_9O_20. Ceramics with theoretical density of ≥95% were achieved in all cases after sintering at 1300 °C. A pure Ba_2Ti_9O_20 phase was obtained by treating the material with TiO_2 additions (≤5.6 wt.%) and sintering at temperatures ranging between 1200 and 1350 °C. Ba_2Ti_9O_20 is a nonstoichiometric compound that can accommodate an excess amount of TiO_2. As the temperature was increased, pure Ba_2Ti_9O_20 partially decomposed and formed a mixture of BaTi_4O_9 and Ba_2Ti_9O_20. The ceramic with excess TiO_2 sintered at 1390 °C possessed a higher permittivity and a lower quality factor due to the larger grain size and lower density. For ceramic with the addition of ZrO_2 (≤6 wt.%), pure Ba_2Ti_9O_20 phase was obtained after sintering between 1200 and 1390 °C, and the quality factor was improved. The decomposition temperature of the Ba_2Ti_9O_20 phase was greater than 1390 °C. For sintering temperatures ≥1350 °C, the extent of Ba_2Ti_9O_20 phase decreased with MnO additions. As the MnO content reached 0.5 wt. %, only BaTi_4O_9 and TiO_2 phases existed, suggesting a decrease in the decomposition temperature of Ba_2Ti_9O_20 with the addition of MnO. The microwave properties of the ceramics degraded significantly at the sintering temperature of 1390 °C.
Pclust: protein network visualization highlighting experimental data

SUMMARY One approach to infer functions of new proteins from their homologs utilizes visualization of an all-against-all pairwise similarity network (A2ApsN) that exploits the speed of BLAST and avoids the complexity of multiple sequence alignment. However, identifying functions of the protein clusters in A2ApsN is never trivial, due to a lack of linking characterized proteins to their relevant information in current software packages. Given the database errors introduced by automatic annotation transfer, functional deduction should be made from proteins with experimental studies, i.e. 'reference proteins'. Here, we present a web server, termed Pclust, which provides a user-friendly interface to visualize the A2ApsN, placing emphasis on such 'reference proteins' and providing access to their full information in source databases, e.g. articles in PubMed. The identification of 'reference proteins' and the ease of cross-database linkage will facilitate understanding the functions of protein clusters in the network, thus promoting interpretation of proteins of interest. AVAILABILITY The Pclust server is freely available at http://prodata.swmed. edu/pclust
Multi-user searchable encryption scheme with general access structure

Searchable encryption is a cryptographic primitive that enables searching over encrypted data. Think over an organization that has outsourced its encrypted database. Consider the scenario in which each member of this organization can add encrypted data to the database but accessing the data is limited to certain predefined access structure. That is, only the members of authorized subsets of members can collaborate to search for a desired data and then decrypt the received ciphertexts. In this paper, we construct a scheme that enables such a scenario and supports general access structure.
Is Resistance to Dolutegravir Possible When This Drug Is Used in First-Line Therapy?

Dolutegravir (DTG) is an HIV integrase inhibitor that was recently approved for therapy by the Food and Drug Administration in the United States. When used as part of first-line therapy, DTG is the only HIV drug that has not selected for resistance mutations in the clinic. We believe that this is due to the long binding time of DTG to the integrase enzyme as well as greatly diminished replication capacity on the part of viruses that might become resistant to DTG. We further speculate that DTG might be able to be used in strategies aimed at HIV eradication.
Multiplexing-based polarization sensitive en-face optical coherence tomography

Abstract. We present a time-domain polarization-sensitive (PS) optical coherence tomography configuration operating at 830 nm, equipped with multichannel acousto-optic deflectors and single photodetectors. The system is used to simultaneously acquire interference information from multiple PS channels and to enable measurement and imaging of backscattered intensity to create both PS and polarization insensitive images. Our approach enables multiple channel imaging without need to divide the object signal. Here, we employ our system in order to demonstrate PS imaging of a thermally damaged muscle tissue.
The effect of composition on morphological, thermal, and mechanical properties of polypropylene/nylon-6/polypropylene-g-butyl acrylate blends

The effect of concentration of polypropylene grafted with butyl acrylate (PP-g-BA) compatibilizer on thermal, morphological, and mechanical properties of polypropylene/nylon-6 blends has been studied. It was observed that blends with 4.8 wt% concentration of PP-g-BA exhibited superior mechanical and morphological properties at all the compositions of PP/Ny-6. Attempts were made to correlate the experimental data for tensile modulus and strength with existing mathematical models.
Design considerations for a computationally-lightweight authentication mechanism for passive RFID tags

Abstract : Passive RFID tags are attractive for their low cost, small footprint, and ability to function without batteries. The lack of onboard power, however, limits the complexity of operations that can be performed by the tags? integrated circuits, and this limitation prevents the tags from being able to perform typical functions required to support e-authentication. This thesis quantifies the delta between the power that would be required to perform MAC-based authentication, and the power made available to a tag via the reader. A modified MAC protocol is then proposed that would theoretically close this delta while still providing sufficient authentication assurance.
Evidence for Distinct Functions of MRE11 in Arabidopsis Meiosis

The evolutionary conserved Mre11/Rad50/Nbs1 complex functions as one of the guardians of genome integrity in eukaryotes; it is required for the double-strand break repair, meiosis, DNA checkpoint, and telomere maintenance. To better understand the role of the MRE11 gene in Arabidopsis, we performed comparative analysis of several mre11 alleles with respect to genome stability and meiosis. The mre11-4 and mre11-2 alleles presumably produce truncated MRE11 proteins composed of the first 499 and 529 amino acids, respectively. Although the putative MRE11 truncated proteins differ only by 30 amino acids, the mutants exhibited strikingly different phenotypes in regards to growth morphology, genome stability and meiosis. While the mre11-2 mutants are fully fertile and undergo normal meiosis, the mre11-4 plants are sterile due to aberrant repair of meiotic DNA breaks. Structural homology analysis suggests that the T-DNA insertion in the mre11-4 allele probably disrupted the putative RAD50 interaction and/or homodimerization domain, which is assumed to be preserved in mre11-2 allele. Intriguingly, introgression of the atm-2 mutant plant into the mre11-2 background renders the double mutant infertile, a phenotype not observed in either parent line. This data indicate that MRE11 partially compensates for ATM deficiency in meiosis of Arabidopsis.
Kidney allograft failure due to acute phosphate nephropathy associated with severe secondary hyperparathyroidism

Intratubular calcification is a common finding in renal allografts. However, possible harmful effect of this calcification is not well recognized, and allograft failure purely due to this condition has not been reported. We report a kidney transplant recipient who suffered from severe secondary hyperparathyroidism and unexplained early allograft failure. A diagnosis of acute phosphate nephropathy was made subsequently based on serial allograft biopsy findings. This case calls for a high index of suspicion to look for this rare cause of allograft dysfunction among high-risk patients. It also highlights the importance of good calcium–phosphate control before renal transplantation.
Extending a wordnet framework for simplicity and scalability

The WordNet knowledge model is currently implemented in multiple software frameworks providing procedural access to language instances of it. Frameworks tend to be focused on structural/design aspects of the model thus describing low level interfaces for linguistic knowledge retrieval. Typically the only high level feature directly accessible is word lookup while traversal of semantic relations leads to verbose/complex combinations of data structures, pointers and indexes which are irrelevant in an NLP context. Here is described an extension to the JWNL framework that hides technical requirements of access to WordNet features with an essentially word/sense based API applying terminology from the official online interface. This high level API is applied to the original English version of WordNet and to an SQL based Portuguese lexicon, translated into a WordNet based representation usable by JWNL.
Intelligent frame refresh for energy-aware display subsystems in mobile devices

Frame refreshes, that are used to retain frame images from frame buffers for display subsystems in mobile devices, waste energy and memory bandwidth. In this paper, we propose an intelligent frame refresh mechanism to reduce redundant frame refreshes and useless data accesses to frame buffers, which bridges the semantic gap between frame buffers and frame refreshes, and exploits the knowledge of frame buffers to guide frame refreshes. Based on this mechanism, we introduce two detailed schemes to optimize refreshes by utilizing different information. The flipping-aware frame refresh scheme uses the frame buffer switching operations to detect frame image updates and triggers useful refreshes. The row-level frame refresh scheme supports to refresh only modified rows instead of the whole frame, under the guidance of pixel status information of frame buffers. Our evaluation results show that our proposed mechanism can reduce memory requests by nearly 50% and memory power consumption up to 30%, compared to conventional fixed frame refresh mechanism.
Transcoronary Ethanol Ablation for Recurrent Ventricular Tachycardia After Failed Catheter Ablation: An Update

Background— Despite substantial progress, radiofrequency catheter ablation (RFCA) fails in some patients. After encouraging results with transcoronary ethanol ablation (TCEA), we began offering TCEA routinely when endocardial and epicardial RFCA failed or a deep intramural substrate was likely. Methods and Results— Among 274 consecutive patients who underwent 408 ventricular tachycardia (VT) ablation procedures, 27 patients (21 men; age, 63±13 years; left ventricular ejection fraction, 30±11%; ischemic cardiomyopathy, 14) had 29 TCEA procedures attempted. In 5 patients, TCEA was abandoned because of unfavorable anatomy. In 22 patients, a mean of 1.3±0.6 arteries (range, 1–3 arteries) were targeted for TCEA. After ablation, the targeted VT was no longer inducible in 18 of 22 (82%) patients. Complete heart block occurred in 5 patients, and 3 patients with advanced heart failure died within 30 days of the procedure. After the last TCEA procedure, a VT recurred in 64% of patients, and overall, 32% of patients died. Of 11 patients with prior VT storm, 9 were free of VT storm. At repeat study in 8 patients who had a recurrence, 7 had a new QRS morphology of VT originating from the same general substrate region as the prior VT. Conclusions— In patients with difficult-to-control VT in whom RFCA fails, TCEA prevents all VT recurrences in 36% and improves arrhythmia control in an additional 27%. Inadequate target vessels, collaterals, and recurrence of modified VTs limit efficacy, but TCEA continues to play an important role for difficult VTs in these high-risk patients.
Multi-user detection using space-time coding to improve systems capacity

A low-complexity probabilistic data association (PDA) multi-user detection algorithm is derived for space-time block coded multi-user systems, which can enhance the capacity of communication system by introducing co-channel users. Considered synchronous co-channel multi-users, each one is equipped with two transmit and two receive antennas, and then extended to multiple receive antennas to improve the performance. The simulation results show that the detection algorithm can reduce the intersymbol interference (ISI) greatly, and the system performance can get much improvement by using this strategy, which is very important for enlarging the communication capacity.
Singularity links with exotic Stein fillings

In a recent paper of Akhmedov, Etnyre, Mark and Smith, it was shown that there exist infinitely many contact Seifert fibered 3-manifolds each of which admits infinitely many exotic (homeomorphic but pairwise non-diffeomorphic) simply-connected Stein fillings. Here we extend this result to a larger set of contact Seifert fibered 3-manifolds with many singular fibers and observe that these 3-manifolds are singularity links. In addition, we prove that the contact structures induced by the Stein fillings are the canonical contact structures on these singularity links. As a consequence, we verify a prediction of Andras Nemethi by providing examples of isolated complex surface singularities whose links with their canonical contact structures admitting infinitely many exotic simply-connected Stein fillings. Moreover, for infinitely many of these contact singularity links and for each positive integer n, we also construct an infinite family of exotic Stein fillings with fixed fundamental group $\mathbb{Z} \oplus \mathbb{Z}_n$.
Optimal subpixel matching of contour chains and segments

This paper introduces a new general purpose algorithm that allows the optimal geometric match between contours to be determined, that is the transformation yielding a minimal deformation is obtained. The algorithm relies only on the geometric properties of the contours and does not call for any other constraint, so that it is particularly suitable when no parameterization of title deformation is available or desirable. Contour deformation is explicitly incorporated in the computation, allowing for a thorough use of all geometric information available. Moreover, no discretization is involved in the computation, resulting in two main advantages: first, the algorithm is robust to differences in the segmentation of contours and allows the matching of polygonal approximations of contours with very little loss of precision, second, subpixel precision matching can be achieved. <<ETX>>
The Overexpression of TDP-43 Protein in the Neuron and Oligodendrocyte Cells Causes the Progressive Motor Neuron Degeneration in the SOD1 G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis

The recent investigation suggested that the TDP-43 protein was closely related to the motor neuron degeneration in amyotrophic lateral sclerosis (ALS), but the pathogenesis contributed to motor neuron degeneration largely remained unknown. Therefore, we detected the alteration of TDP-43 expression and distribution in the adult spinal cord of the SOD1 G93A transgenic mouse model for searching the possible pathogenesis of ALS. We examined the TDP-43 expression and distribution in the different anatomic regions, segments and neural cells in the adult spinal cord at the different stages of the SOD1 wild-type and G93A transgenic model by the fluorescent immunohistochemical technology. We revealed that the amount of TDP-43 positive cell was cervical>lumbar>thoracic segment, that in the ventral horn was more than that in the dorsal horn, a few of TDP-43 protein sparsely expressed and distributed in the other regions, the TDP-43 protein weren't detected in the white matter and the central canal. The TDP-43 protein was mostly expressed and distributed in the nuclear of neuron cells and the cytoplasm of oligodendrocyte cells of the gray matter surrounding the central canal of spinal cord by the granular shape in the SOD1 wild-type and G93A transgenic mice. The amount of TDP-43 positive cell significantly increased at the onset and progression stages of ALS following with the increase of neuron death in spinal cord, particularly in the ventral horn of cervical segment at the progression stage. Our results suggested that the overexpression of TDP-43 protein in the neuron and oligodendrocyte cell causes the progressive motor neuron degeneration in the ALS-like mouse model.
University of Birmingham Dimerization of inositol monophosphatase Mycobacterium tuberculosis SuhB is not constitutive, but induced by binding of the activator Mg2+

Background: The cell wall of Mycobacterium tuberculosis contains a wide range of phosphatidyl inositol-based glycolipids that play critical structural roles and, in part, govern pathogen-host interactions. Synthesis of phosphatidyl inositol is dependent on free myo-inositol, generated
Development of Oxide-Based EBC for Silicon Nitride

In search of a candidate EBC material for silicon nitride ceramics, water vapor corrosion test was performed for a series of monolithic materials such as Al2O3, TiO2, ZrO2, HfO2, Al2TiO5, Al6Si2O13, Lu4Hf3O12, Ln2Si2O7 (Ln = Yb and Er) and mullite/Lu2Si2O7 eutectic bulk at above 1300°C. Based on the high-temperature water vapor corrosion test results and the resultant microstructures, the mechanisms of water vapor corrosion of these materials were discussed in this paper. In this present study, ZrO2 and HfO2 showed excellent resistance to water vapor corrosion at high temperatures. Among low-thermal-expansion materials, specifically silicate-based materials, Lu2Si2O7 showed better results in the aspect of weight loss rate during the corrosion test, which also desirably has a coefficient of thermal expansion very close to that of silicon nitride. To verify the recession mechanism of Lu2Si2O7, a recession test was performed for this bulk in high-velocity combustion gas flow at 1300-1500°C. This material showed excellent recession resistance compared to Al2O3 and Si3N4. Different coating techniques, such as sputtering, sol-gel, and reaction sintering, were employed to coat a high-density Lu2Si2O7 EBC layer on a silicon nitride substrate. However, in polycrystalline Lu2Si2O7 phase, the grain boundary silica was prone to water vapor corrosion. It was observed that the corrosion resistance of a Lu2Si2O7/mullite eutectic bulk, without boundary silica, was more excellent than that of polycrystalline Lu2Si2O7 bulk.
Changes in DNA Synthesis Rate in the Schwann Cell Lineage In Vivo Are Correlated With the Precursor – Schwann Cell Transition and Myelination

During the development of the rat sciatic nerve extensive proliferation of glial cells occurs, and there is a very substantial rearrangement of the cytoarchitecture as axons and Schwann cells assume relationships which lead to the formation of the myelinated and unmyelinated axons characteristic of adult nerve. The maturation of Schwann cells from Schwann cell precursors and the matching of Schwann cell numbers to axons is an important part of this process. We have therefore studied the proliferation of Schwann cell precursors and Schwann cells during the development of the rat sciatic nerve from embryonic day 14 to postnatal day 28 by combining bromodeoxyuridine injections of rats with double‐label immunohistochemical techniques. The results reveal that DNA synthesis occurs in both Schwann cell precursors and Schwann cells throughout early nerve development. The labelling index is already substantial at embryonic day 14, but from embryonic day 17, when essentially all the glial cells have converted from precursor to Schwann cell phenotype, it rises sharply, peaking between embryonic day 19 and 20 before declining precipitously in the early postnatal period. This rapid decline in DNA synthesis coincides with the appearance of the myelin protein Po, and in individual cells DNA synthesis is incompatible with the expression of Po protein. Non‐myelin‐forming Schwann cells, which mature later in development, continue to synthesize DNA until at least postnatal day 15, but by day 28 essentially all Schwann cells in the nerve are quiescent.
Prolonged depolarization elicited in Purkinje cell dendrites by climbing fibre impulses in the cat.

1. Responses evoked in Purkinje cell dendrites by impulses in climbing fibres were studied by recording from the cat cerebellar cortex. Intra‐ and extracellular responses from dendrites of single Purkinje cells were recorded, as well as field responses from the intact cerebellar surface. The intracellular responses were presumably recorded from relatively proximal dendrites. The resting potential usually was 20‐40 mV. The responses consisted of an initial spike‐like component (amplitude 10‐30 mV) followed by a plateau‐like component (amplitude 2‐12 mV) with a duration of about 100 ms. The duration of consecutive responses varied little. 3. The extracellular unitary responses were recorded from more distal dendrites. These responses were negative deflexions consisting of an initial component followed by a plateau‐like component (amplitude 5‐15 mV). The duration of consecutive responses varied widely from about 25 ms to more than 1 s. The negative deflexions are assumed to correspond to dendritic depolarizations. 4. The field responses recorded from the cerebellar surface consisted of a positivity followed by a negativity lasting several hundred milliseconds. The positivity signals the e.p.s.p.s generated by the climbing fibre synapses which do not extend to the most distal dendrites. The negativity presumably signals the plateau‐like dendritic depolarizations which would involve also the most distal dendrites. 5. The nature and significance of the plateau‐like depolarizations evoked by climbing fibre impulses in the purkinje cell dendrites are discussed.
Editors’ Choice papers in Thrombosis and Haemostasis

It was five years ago that we decided to feature our Editors’ Choice article for the first time. This article comprises a fine selection of articles in our journal that best reflect the most relevant and highly cited content and represent the scope of hot topics and developments over the past year. Since then, we have also enjoyed a steady growth of contributing authors that have been attracted by the journal’s impact and this is paralleled by a corresponding and continuous increase in article submissions, of which we have annually received more than 1,000 over the past two years. One aspect deserving particular attention is that a major part of highly cited papers published in the recent two years has been contributed by the authors of our Theme Issues. In part, this rewards their efforts to identify and to introduce new and promising themes and to systematically elaborate on such exciting and recent developments. Some of the most highly cited papers of the past two years will be succinctly highlighted and summarised herein.