Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibition of TATA-Binding Protein Function by SAGA Subunits Spt3 and Spt8 at Gcn4-Activated Promoters

ABSTRACT SAGA is a 1.8-MDa yeast protein complex that is composed of several distinct classes of transcription-related factors, including the adaptor/acetyltransferase Gcn5, Spt proteins, and a subset of TBP-associated factors. Our results indicate that mutations that completely disrupt SAGA (deletions of SPT7 orSPT20) strongly reduce transcriptional activation at theHIS3 and TRP3 genes and that Gcn5 is required for normal HIS3 transcriptional start site selection. Surprisingly, mutations in Spt proteins involved in the SAGA-TBP interaction (Spt3 and Spt8) cause derepression of HIS3 andTRP3 transcription in the uninduced state. Consistent with this finding, wild-type SAGA inhibits TBP binding to theHIS3 promoter in vitro, while SAGA lacking Spt3 or Spt8 is not inhibitory. We detected two distinct forms of SAGA in cell extracts and, strikingly, one lacks Spt8. Conditions that induceHIS3 and TRP3 transcription result in an altered balance between these complexes strongly in favor of the form without Spt8. These results suggest that the composition of SAGA may be dynamic in vivo and may be regulated through dissociable inhibitory subunits.
Principles to control autonomous formation merging

This paper provides a complete description of possible scenarios of merging two minimally rigid or globally rigid formations to obtain a single minimally rigid or globally rigid formation, respectively, both in Ropf2 and Ropf3. A strategy is developed based on simplification of the merging problem to a problem of growing a minimally or globally rigid graph. Based on this strategy, three principles are provided to control the merging efficiently and optimally, in the sense of minimizing the number of added edges and the number of vertices incident to these edges. Any possible scenario of merging two given minimally or globally rigid formations can be handled using a combination of these three principles. Although the main results are provided for merging two formations, application of the proposed techniques to merging of more than two formations is briefly discussed as well
Exploring social media campaigns against sugar-sweetened beverage consumption: A systematic search

Abstract Significant evidence has supported a positive association between consumption of sugar-sweetened beverage and increased risks of the non-communicable diseases of obesity, metabolic disorders, dental caries, and dental erosion. Thus, using social marketing concepts to change people’s attitude and behaviour towards the consumption is imperative. Social media is considered as a cheap and quick tool to disseminate health messages in health communication. The use of social media has increased significantly but knowledge of its utilization in sugar-sweetened beverage health campaigns is limited. This study was conducted to identify social media health campaigns against the sugar-sweetened beverage consumption, their social media platforms and types of materials distributed, and to identify health messages being highlighted in the campaigns. The authors conducted a systematic search for the campaigns and employed content analysis to identify health messages. As a result, 34 campaigns were identified. Facebook and YouTube were commonly used to disseminate campaign materials—83% of them were videos and text articles. Obesity/overweight, diabetes, and cardiovascular diseases were the most frequently mentioned health messages in the campaigns. The increased use of social media with their low-cost operation and capacity to increase campaign reach makes them potential communication channels for health campaigns against sugar-sweetened beverage consumption.
Optimized Rostering of Workforce Subject to Cyclic Requirements

SNCF is a large railway transportation company that operates 365 days a year and 24 hours a day. In order to schedule a certain category of workers at train stations and ticket selling points, rosters are designed to cover a cyclical demand. However, the highly combinatorial nature of the rostering problem makes it very difficult to solve it manually, and experts spend a huge amount of time to make them legally feasible and to improve a certain number of preference criteria. This paper presents a mixed-integer programming model to address the cyclical rostering problem using patterns corresponding to feasible blocks of seven days and assigning them to each week of the roster. Some valid inequalities are presented to improve the linear relaxation of the model and thereby enhance computational performance. Implementation results are presented, including comparisons with an alternative daily-variables model Povzetek: Opisano je optimirano cikli? no razporejanje delavcev z aplikacijo za podjetje SNCF.
2 The Extant Phylogenetic Bracket and the importance of reconstructing soft tissues in fossils

Fossils usually provide paleontologists with little more than bones and teeth as primary data. Because the broad aim of functional morphological analyses of extinct organisms is to breathe life into fossils, paleontologists are challenged to reconstruct those portions of the animal not normally prese~ed in the fossil record in particular, soft tissues. Soft-tissue considerations are important for two major reasons: (1) Soft tissues often have korphogenetic over skeletal tissues, and (2) such considerations often lie at . , the base of numerous paleobiological inferences. Furthermore, soft-tissue analysis can provide causal hypotheses of phylogenetic character correlation. A methodology for reconstructing soft anatomy in fossils the Extant Phylogenetic Bracket approach is proposed here. This method makes explicit reference to at least the first two extant outgroups of the fossil taxon of interest. It is based firmly on the relation of biological homology, provides a means of rigorously establishing the limits of our inferences, and allows construction of a hierarchy of inference. The approach can be applied to virtually any unpresemed trait of a fossil taxon with little or no modification.
Commentary: Is Melatonin Administration an Effective Hypnotic?

volunteers undergoing experimental disruption of their sleep (i.e., transient insomnia). The consensus primary outcome measures in these clinical trials are measures of sleep induction (e.g., latency to Stage 2 non-rapid eye movement [non-REM], latency to persistent sleep) and sleep maintenance (e.g., sleep efficiency, number of wakenings, wake time after sleep onset). These measures are assayed using electrophysiological parameters (termed polysomnography) or from patient evaluations via a postsleep questionnaire (Roth et al., 1994).
Composition chimique et propriétés antioxydante et antimicrobienne de l’huile essentielle d’Origanum floribundum Munby

L’objectif du travail est l’étude de la composition chimique de l’huile essentielle (HE) d’Origanum floribundum Munby, ainsi que l’évaluation de son activité antioxydante et antimicrobienne. L’analyse de la composition chimique réalisée par chromatographie phase gazeuse couplée à la spectrométrie de masse a permis d’identifier 27 composés représentant 99,8 % de la composition totale, dont le composant majoritaire est le p-cymène à 53,4 %. Les résultats de l’activité antimicrobienne de l’HE ont montré qu’elle présente un large spectre d’action vis-à-vis de tous les microorganismes testés, ainsi qu’un effet bactéricide et fongicide puissant. La zone d’inhibition la plus importante (50,67 ± 0,58 mm) a été observée chez Candida albicans et la plus faible chez Pseudomonas aeruginosa (13,33 ± 1,44 mm). L’activité d’inhibition du radical DPPH (2,2- diphényl-1-picrylhydrazyle) à 600, 800 et 1 000 mg/l par l’HE est comparable à l’hydroxytoluène butylé (BHT) et au tocophérol (vitamine E), antioxydants de référence, elle reste inférieure aux concentrations de 100, 200 et 400 mg/l avec une valeur IC50 de 500,71 ± 16,97 mg/l. Le pouvoir réducteur de l’HE d’origan aux concentrations (100, 200, 400, 600, 800 et 1 000 mg/l) est légèrement inférieur aux antioxydants de référence retenus pour cette étude.
IQGAP1 and Calmodulin Modulate E-cadherin Function*

Ca2+-dependent cell-cell adhesion is mediated by the cadherin family of transmembrane proteins. Adhesion is achieved by homophilic interaction of the extracellular domains of cadherins on adjacent cells, with the cytoplasmic regions serving to couple the complex to the cytoskeleton. IQGAP1, a novel RasGAP-related protein that interacts with the cytoskeleton, binds to actin, members of the Rho family, and E-cadherin. Calmodulin binds to IQGAP1 and regulates its association with Cdc42 and actin. Here we demonstrate competition between calmodulin and E-cadherin for binding to IQGAP1 both in vitro and in a normal cellular milieu. Immunocytochemical analysis in MCF-7 (E-cadherin positive) and MDA-MB-231 (E-cadherin negative) epithelial cells revealed that E-cadherin is required for accumulation of IQGAP1 at cell-cell junctions. The cell-permeable calmodulin antagonist CGS9343B significantly increased IQGAP1 at areas of MCF-7 cell-cell contact, with a concomitant decrease in the amount of E-cadherin at cell-cell junctions. Analysis of E-cadherin function revealed that CGS9343B significantly decreased homophilic E-cadherin adhesion. On the basis of these data, we propose that disruption of the binding of calmodulin to IQGAP1 enhances the association of IQGAP1 with components of the cadherin-catenin complex at cell-cell junctions, resulting in impaired E-cadherin function.
Unusual presentation of left atrial myxoma

A 48-year-old man was admitted with symptoms of low grade fever and lower limb paraesthesia of 5 months duration. He also gave history of bilateral femoral and left brachial embolectomy performed elsewhere 4 months back. Clinical examination was normal. Routine blood investigations including complete haemogram, his liver and renal functions were normal. The coagulation profile was also normal; however, his erythrocyte sedimentation rate was elevated at 86 mm/hour(reference for laboratory;<20 mm/hour). Echocardiographic evaluation (figures …
Swarm pattern transformation methodologies

The work reported in this paper is motivated by the need for developing swarm pattern transformation methodologies. Two methods, namely a macroscopic method and a mathematical method are investigated for pattern transformation. The first method is based on macroscopic parameters while the second method is based on both microscopic and macroscopic parameters. A formal definition to pattern transformation considering four special cases of transformation is presented. Simulations on a physics simulation engine are used to confirm the feasibility of the proposed transformation methods. A brief comparison between the two methods is also presented.
A priori computation of the number of surface subdivision levels

Subdivision surfaces are a powerful model widely used in geometric modelling. Controlling the accuracy of the approximation of the limit surface often involves the computation of the distance between the control mesh and the limit surface. Nevertheless, the a priori level (or depth) of subdivision based on a distance criterion has not yet been expressed. The goal of this paper is thus to compute this level. Then the surface can be subdivided with a given accuracy without any distance computation between the subdivision surface and the limit surface.
Metals accelerate production of the aberrant splicing isoform of the presenilin‐2

Oxidative stress is a major risk factor for Alzheimer's disease (AD) and other neurodegenerative disorders. Metals are known to be one of the factors that contribute to oxidative stress. Recently, we reported that the aberrant splicing isoform (PS2V) generated by skipping exon5 of the presenilin‐2 (PS2) gene is a diagnostic feature of sporadic AD (SAD). PS2V is inducible by exposure of human neuroblastoma to hypoxia. We examined whether this aberrant splicing was caused by metal‐induced oxidative stress, such as exposure to aluminum. As a result, we demonstrated that exposure to aluminum accelerated PS2V production induced by hypoxia. This acceleration of the production of PS2V to hypoxia was caused by chronic aluminum exposure, but was not related to the intracellular content of aluminum. HMGA1a is a mediator of PS2V production, and it was induced by aluminum as well as by hypoxia. Induction of HMGA1a was increased by chronic exposure to aluminum, and a nuclear extract containing HMGA1a bound to a specific sequence on exon5 of PS2 pre‐mRNA, as reported previously. Finally, the acceleration of PS2V production induced by aluminum under hypoxic conditions reflected, but has not yet been directly shown to cause, vulnerability to endoplasmic reticulum stress. These results suggest that exposure to some metals can accelerate and enhance PS2V generation, and that hypoxia plus chronic exposure to metals may promote the development of AD.
What's left in asymmetry?

Left–right patterning is a fascinating problem of morphogenesis, linking evolutionary and cellular signaling mechanisms across many levels of organization. In the past 15 years, enormous progress has been made in elucidating the molecular details of this process in embryos of several model species. While many outside the field seem to believe that the fundamental aspects of this pathway are now solved, workers on asymmetry are faced with considerable uncertainties over the details of specific mechanisms, a lack of conceptual unity of mechanisms across phyla, and important questions that are not being pursued in any of the popular model systems. Here, we suggest that data from clinical syndromes, cryptic asymmetries, and bilateral gynandromorphs, while not figuring prominently in the mainstream work on LR asymmetry, point to crucial and fundamental gaps of knowledge about asymmetry. We identify 12 big questions that provide exciting opportunities for fundamental new advances in this field. Developmental Dynamics 237:3453–3463, 2008. © 2008 Wiley‐Liss, Inc.
Impact of the Content of Fatty Acids of Oral Fat Tolerance Tests on Postprandial Triglyceridemia: Systematic Review and Meta-Analysis

Whether the content of saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA) could differently influence postprandial triglycerides (TG) is unknown. We examined possible differences in the postprandial TG response to fat tolerance tests (FTTs), in which SFA or unsaturated fatty acids were used. Crossover clinical trials investigating the effects of FTTs containing SFA and unsaturated fats on postprandial triglyceridemia in databases from 1994 until 2016 were searched. Of 356 studies, 338 were excluded and 18 were considered. TG net incremental areas under the curve were calculated using time-points or changes from baseline. Pooled effects of standardized mean differences and I2 test were used. Results: In 12 studies, responses to SFA versus PUFA meals, and in 16 studies versus MUFA meals were compared. Over 4 h, no differences between SFA and unsaturated fats were observed. Over 8 h a lower response to PUFA (SMD −2.28; 95% CI −4.16, −0.41) and a trend to lower response to MUFA (SMD −0.89, 95% CI −1.82, 0.04) were detected. FTTs shorter than 8 h may not be sufficient to differentiate postprandial TG after challenges with distinct fatty acids. Clinical significance of different postprandial TG responses on cardiovascular risk in the long-term deserves investigation.
Neuroanatomy of Down's syndrome: a high-resolution MRI study.

OBJECTIVE Down's syndrome, the most common genetic cause of mental retardation, results in characteristic physical and neuropsychological findings, including mental retardation and deficits in language and memory. This study was undertaken to confirm previously reported abnormalities of regional brain volumes in Down's syndrome by using high-resolution magnetic resonance imaging (MRI), determine whether these volumetric abnormalities are present from childhood, and consider the relationship between neuroanatomic abnormalities and the cognitive profile of Down's syndrome. METHOD Sixteen children and young adults with Down's syndrome (age range=5-23 years) were matched for age and gender with 15 normal comparison subjects. High-resolution MRI scans were quantitatively analyzed for measures of overall and regional brain volumes and by tissue composition. RESULTS Consistent with prior imaging studies, subjects with Down's syndrome had smaller overall brain volumes, with disproportionately smaller cerebellar volumes and relatively larger subcortical gray matter volumes. Also noted was relative preservation of parietal lobe gray and temporal lobe white matter in subjects with Down's syndrome versus comparison subjects. No abnormalities in pattern of brain asymmetry were noted in Down's syndrome subjects. CONCLUSIONS The results largely confirm findings of previous studies with respect to overall patterns of brain volumes in Down's syndrome and also provide new evidence for abnormal volumes of specific regional tissue components. The presence of these abnormalities from an early age suggests that fetal or early postnatal developmental differences may underlie the observed pattern of neuroanatomic abnormalities and contribute to the specific cognitive and developmental deficits seen in individuals with Down's syndrome.
Specifying ICD9, ICPC and ATC codes for the STOPP/START criteria: a multidisciplinary consensus panel.

BACKGROUND the STOPP/START criteria are a promising framework to increase appropriate prescribing in the elderly in clinical practice. However, the current definitions of the STOPP/START criteria are rather non-specific, allowing undesirable variations in interpretation and thus application. The aim of this study was to design specifications of the STOPP/START criteria into international disease and medication codes to facilitate computerised extraction from medical records and databases. METHODS a three round consensus procedure with a multidisciplinary expert panel was organised to prepare, judge and agree on the design of the STOPP/START criteria specifications in corresponding international disease codes (ICD9 and ICPC) and medication codes (ATC). RESULTS after two rounds consensus was reached for 74% of the STOPP criteria and for 73% of the START criteria. After three rounds full consensus was reached resulting in a specification of 61 out of 62 STOPP criteria and 26 START criteria with their corresponding codes. One criterion could not be specified and for some criteria corresponding disease codes were lacking or imperfect. CONCLUSION this study showed the necessity of a consensus procedure as even experts frequently differed on how to specify the STOPP/START criteria. This specification enables next steps such as prognostic validation of these criteria on adverse outcomes and studying the impact of improving appropriate prescribing in the elderly.
Decision feedback equalizer for CDMA system with low spreading factor

For wideband code-division multiple access (CDMA) systems, we investigate a decision feedback equalizer (DFE) to effectively suppress serious inter-path interference (IPI) when the spreading factor is low. In order to eliminate the error propagation in DFE, a soft decision feedback equalization structure is also studied. The optimized time delay parameter and unbiased estimator are obtained to further improve the DFE performance. The proposed symbol-level DFE has the same performance as compared to chip-level DFE but it has lower complexity and its adaptive realization can further lower the complexity. The result of simulation and analysis show that the performance of the proposed DFE is better than that of linear MMSE equalizer and standard RAKE receiver.
Burden of digestive diseases in the United States part I: overall and upper gastrointestinal diseases.

Introduction to Series Digestive, liver, and pancreatic diseases have a far-reaching medical, economic, social, and political impact on society in the United States and worldwide. We believe it is important to highlight salient features as well as key tables/figures from the Burden of Digestive Diseases in the United States report developed as part of the National Commission on Digestive Diseases of the National Institutes of Health. The commission was created, in part, due to the advocacy efforts of the AGA. Divided into three segments, this series, spanning consecutive issues in Gastroenterology, will be informative and illuminating. The series will provide investigators, clinicians, and other readers with the necessary tools to generate new hypotheses, craft new directions of broad-based and integrated research, and make informed clinical conclusions. Hashem B. El-Serag, Associate Editor Anil K. Rustgi, Editor
Generic robust LVCMOS-compatible control logic for GaAs HEMT switches

Robust digital control logic for an X-band five-bit digital attenuator and six-bit phase shifter has been developed and tested. The circuit uses an innovative combination of feedback and feed forward. The devices have been developed in the 6-inch 0.5 mum power pHEMT process (PP50-10) of WIN Semiconductors. The use of this process results in cost effective devices with a high power capability
On the Complexity of Extending Ground Resolution with Symmetry Rules

One important issue of automated theorem proving is the complexity of the inference rules used in theorem provers. If Krishnamurty's general symmetry rule is to be used, then one should provide some way of computing non trivial symmetries. We show that this problem is NP-complete. But this general rule can be simplified by restricting it to what we call 5- symmetries, yielding the well-known symmetry rule. We show that computing 5-symmetries is in the same complexity class as the graph isomorphism problem, which appears to be neither polynomial nor NP-complete. However it is sufficient to compute the set of all 5-symmetries at the beginning of the proof search, and since it is a permutation group, there exist some efficient techniques from computational group theory to represent this set. We also show how these techniques can be used for applying the S-symmetry rule in polynomial time.
Packing Short Plane Spanning Trees in Complete Geometric Graphs

Given a set of points in the plane, we want to establish a connection network between these points that consists of several disjoint layers. Motivated by sensor networks, we want that each layer is spanning and plane, and that no edge is very long (when compared to the minimum length needed to obtain a spanning graph). We consider two different approaches: first we show an almost optimal centralized approach to extract two trees. Then we show a constant factor approximation for a distributed model in which each point can compute its adjacencies using only local information. This second approach may create cycles, but maintains planarity.