Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of subinhibitory concentrations of azithromycin on adherence of Pseudomonas aeruginosa to bronchial mucins collected from cystic fibrosis patients.

Sir, Pseudomonas aeruginosa, an opportunistic pathogen, is the major pathogen in the airways of patients with cystic fibrosis (CF) and is currently associated with the morbidity and mortality seen in this disease. Macrolides, such as azithromycin, are normally not included in the anti-pseudomonal therapeutic arsenal because of the absence of bactericidal or bacteriostatic activity. However, several previous investigators have reported that long-term administration of azithromycin is effective in patients with pulmonary P. aeruginosa infections. 1 The clinical benefits achieved could include the effects of an anti-inflammatory 2 and/or modulation of the production of virulence factors of P. aeruginosa, such as bacterial exoproducts, 3 the formation of biofilm, 4 or the synthesis of flagella 3 and some outer membrane proteins. 5 Adherence of P. aeruginosa to respiratory mucins plays an important role in the colonization of the airways of these patients. This adherence process involves flagella and several non-pilus adhesins localized on the outer membrane of P. aeruginosa. Supporting the clinical observations, adherence of this organism to human salivary or airway mucins has been demonstrated in vitro using liquid- or solid-phase adherence assays. 6 In order to investigate the action of azithromycin on the pathogenesis of P. aeruginosa, we evaluated—in a solid-phase adherence assay— the action of subinhibitory concentrations of the macrolide on the adherence of different bacterial strains to respiratory mucins of CF patients. The reference strain PAO-1, and 13 strains (five mucoid and eight non-mucoid strains) freshly isolated from the sputum of CF patients known to be infected by different strains and who were not treated with azithromycin, were tested. MICs of azithromycin were determined by an agar dilution method, following the recommendations of the NCCLS (2002), with powdered azithromycin supplied by Pfizer laboratories (Groton, CN, USA). Adherence assays were performed in 96-well microtitre plates (Linbro, INC laboratories, France), in which wells were coated with 10 µg of purified bronchial mucins prepared from tracheobronchial secretions of CF patients. As described by Vishwanath & Ramphal,6 100 µL of a suspension of ∼1 × 106 bacteria/mL, grown while agitating for 18 h
P3: Joint optimization of charger placement and power allocation for wireless power transfer

Wireless power transfer is a promising technology to extend the lifetime of, and thus enhance the usability of, the energy-hungry battery-powered devices. It enables energy to be wirelessly transmitted from power chargers to energy receiving devices. Existing studies have mainly focused on maximizing network lifetime, optimizing charging efficiency, minimizing charging delay, etc. Different from these works, our objective is to optimize charging quality in a 2-D target area. Specifically, we consider the following charger Placement and Power allocation Problem (P3): Given a set of candidate locations for placing chargers, find a charger placement and a corresponding power allocation to maximize the charging quality, subject to a power budget. We prove that P3 is NP-complete. We first study P3 with fixed power levels, for which we propose a (1-1/e)-approximation algorithm; we then design an approximation algorithm of factor 1-1/e / 2L for P3, where e is the base of the natural logarithm, and L is the maximum power level of a charger. We also show how to extend P3 in a cycle. Extensive simulations demonstrate that, the gap between our design and the optimal algorithm is within 4.5%, validating our theoretical results.
Functional Interaction between the pp71 Protein of Human Cytomegalovirus and the PML-Interacting Protein Human Daxx

ABSTRACT The tegument protein pp71 (UL82) of human cytomegalovirus (HCMV) has previously been shown to transactivate the major immediate-early enhancer-promoter of HCMV. Furthermore, this protein is able to enhance the infectivity of viral DNA and to accelerate the infection cycle, suggesting an important regulatory function during viral replication. To gain insight into the underlying mechanisms that are used by pp71 to exert these pleiotropic effects, we sought for cellular factors interacting with pp71 in a yeast two-hybrid screen. Here, we report the isolation of the human Daxx (hDaxx) protein as a specific interaction partner of HCMV pp71. hDaxx, which was initially described as an adapter protein involved in apoptosis regulation, has recently been identified as a nuclear protein that interacts and colocalizes with PML in the nuclear domain ND10. In order to assess whether pp71 can also be detected in ND10 structures, a vector expressing pp71 in fusion with the green fluorescent protein was used for transfection of human fibroblasts. This revealed a colocalization of pp71 with the ND10 proteins PML and Sp100. In addition, cotransfection of a hDaxx expression vector resulted in an enhanced recruitment of pp71 to ND10. Targeting of pp71 to nuclear dots could also be observed in infected human fibroblasts in the absence of de novo viral protein synthesis. Moreover, cotransfection experiments revealed that pp71-mediated transactivation of the major immediate-early enhancer-promoter was synergistically enhanced in the presence of hDaxx. These results suggest an important role of hDaxx for pp71 protein function.
The influence of lay theories of Feng Shui on consumers' peace of mind: The role of regulatory fit: Feng Shui and regulatory fit

With its history dating back five millennia, the art of creating harmonious surroundings – commonly referred to as Feng Shui – has become deeply rooted in Chinese culture. Yet despite its significant effect on people's daily lives, a dearth of research is available on how Feng Shui influences consumers' decisions. This study investigates the influence of Feng Shui on customers' attitude based on their regulatory focus, providing suggestions for business opportunities. Three studies examine whether Feng Shui's goals influence participants' decisions and feelings of appropriateness. Study 1 demonstrated that the fit between Feng Shui's suggestions and consumers' regulatory focus impacted decision making. Participants were more likely to adopt the Feng Shui practitioner's suggestions of a goal compatible with the consumers' regulatory focus. Study 2 provided evidence that a regulatory focus also impacts participants' peace of mind. Study 3 tested whether the value experienced from regulatory fit is reflected in the price. Specifically, promotion-oriented individuals feel more accepting of promotion-focused Feng Shui that is consistent with an approach goal while prevention-oriented individuals are more persuaded by prevention-focused Feng Shui that is consistent with an avoidance goal. When the Feng Shui appeal is compatible with the self-regulatory focus, individuals demonstrate a greater feeling of appropriateness and produce a higher level of peace of mind.
Synergistic Transcriptional Activation by TATA-Binding Protein and hTAFII28 Requires Specific Amino Acids of the hTAFII28 Histone Fold

ABSTRACT Coexpression of the human TATA-binding protein (TBP)-associated factor 28 (hTAFII28) with the altered-specificity mutant TBP spm3 synergistically enhances transcriptional activation by the activation function 2 of the nuclear receptors (NRs) for estrogen and vitamin D3 from a reporter plasmid containing a TGTA element in mammalian cells. This synergy is abolished by mutation of specific amino acids in the α2-helix of the histone fold in the conserved C-terminal region of hTAFII28. Critical amino acids are found on both the exposed hydrophilic face of this helix and the hydrophobic interface with TAFII18. This α-helix of hTAFII28 therefore mediates multiple interactions required for coactivator activity. We further show that mutation of specific residues in the H1′ α-helix of TBP either reduces or increases interactions with hTAFII28. The mutations which reduce interactions with hTAFII28 do not affect functional synergy, whereas the TBP mutation which increases interaction with hTAFII28 is defective in its ability to synergistically enhance activation by NRs. However, this TBP mutant supports activation by other activators and is thus specifically defective for its ability to synergize with hTAFII28.
Intradermal Indocyanine Green for In Vivo Fluorescence Laser Scanning Microscopy of Human Skin: A Pilot Study

Background In clinical diagnostics, as well as in routine dermatology, the increased need for non-invasive diagnosis is currently satisfied by reflectance laser scanning microscopy. However, this technique has some limitations as it relies solely on differences in the reflection properties of epidermal and dermal structures. To date, the superior method of fluorescence laser scanning microscopy is not generally applied in dermatology and predominantly restricted to fluorescein as fluorescent tracer, which has a number of limitations. Therefore, we searched for an alternative fluorophore matching a novel skin imaging device to advance this promising diagnostic approach. Methodology/Principal Findings Using a Vivascope®-1500 Multilaser microscope, we found that the fluorophore Indocyanine-Green (ICG) is well suited as a fluorescent marker for skin imaging in vivo after intradermal injection. ICG is one of few fluorescent dyes approved for use in humans. Its fluorescence properties are compatible with the application of a near-infrared laser, which penetrates deeper into the tissue than the standard 488 nm laser for fluorescein. ICG-fluorescence turned out to be much more stable than fluorescein in vivo, persisting for more than 48 hours without significant photobleaching whereas fluorescein fades within 2 hours. The well-defined intercellular staining pattern of ICG allows automated cell-recognition algorithms, which we accomplished with the free software CellProfiler, providing the possibility of quantitative high-content imaging. Furthermore, we demonstrate the superiority of ICG-based fluorescence microscopy for selected skin pathologies, including dermal nevi, irritant contact dermatitis and necrotic skin. Conclusions/Significance Our results introduce a novel in vivo skin imaging technique using ICG, which delivers a stable intercellular fluorescence signal ideal for morphological assessment down to sub-cellular detail. The application of ICG in combination with the near infrared laser opens new ways for minimal-invasive diagnosis and monitoring of skin disorders.
Regulation of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharides by CO2

ABSTRACT Staphylococcus aureus expression of capsular polysaccharide type 5 (CP5) has been shown to be downregulated by CO2. Here we show that CO2 reduces CP5 expression at the transcriptional level and that CO2regulates CP8 expression depending on the genetic background of the strains. Growth in the presence of air supplemented with 5% CO2 caused a significant decrease in CP8 expression in fourS. aureus strains, a marginal effect in four strains, and higher CP8 expression in strain Becker. Absolute CP8 expression in the nine S. aureus strains differed largely from strain to strain. Four groups of strains were established due to sequence variations in the promoter region of cap5 andcap8. To test whether these sequence variations are responsible for the different responses to CO2, promoter regions from selected strains were fused to the reporter genexylE in pLC4, and the plasmids were electrotransformed into strains Becker and Newman. XylE activity was negatively regulated by CO2 in all derivatives of strain Newman and was always positively regulated by CO2 in all derivatives of strain Becker. Differences in promoter sequences did not influence the pattern of CP8 expression. Therefore, the genetic background of the strains rather than differences in the promoter sequence determines the CO2 response. trans-acting regulatory molecules may be differentially expressed in strain Becker versus strain Newman. The strain dependency of the CP8 expression established in vitro was also seen in lung tissue sections of patients with cystic fibrosis infected with CP8-positive S. aureus strains.
A Simple Hybrid Aligner for Generating Lexical Correspondences in Parallel Texts

We present an algorithm for bilingual word alignment that extends previous work by treating multi-word candidates on a par with single words, and combining some simple assumptions about the translation process to capture alignments for low frequency words. As most other alignment algorithms it uses cooccurrence statistics as a basis, but differs in the assumptions it makes about the translation process. The algorithm has been implemented in a modular system that allows the user to experiment with different combinations and variants of these assumptions. We give performance results from two evaluations, which compare will with results reported in the literature.
Protective Immunization against Group B Meningococci Using Anti-Idiotypic Mimics of the Capsular Polysaccharide

Use of the serogroup B meningococcal capsular polysaccharide (MenB CP) as a vaccine is hampered by the presence of epitopes that cross-react with human polysialic acid. As non-cross-reactive, protective capsular epitopes have also been described, we set out to develop protein mimics of one of such epitopes using as a template a highly protective mAb (mAb Seam 3) raised against a chemically modified form of the MenB CP (N-Pr MenB CP). Using phage display, anti-idiotypic single-chain Ab fragments (scFvs) were obtained from spleen cells of mice immunized with the Seam 3 mAb. Two Seam 3-specific scFvs competed with N-Pr MenB CP for binding to either mAb Seam 3 or rabbit Abs present in typing sera. Moreover, in mice and rabbits the scFvs elicited the production of Abs reacting with both N-Pr MenB CP and whole meningococci, but not with human polysialic acid. These scFv-induced Ab responses were boostable and of the Th1 type, as shown by a predominance of IgG2a. In addition, passive immunization with sera from scFv-immunized animals partially protected neonatal mice from experimental infection with group B meningococci. In conclusion, we have produced anti-idiotypic scFvs that mimic a protective MenB CP epitope and may be useful in the development of an alternative group B meningococcal vaccine.
Predictive Auto-scaling Techniques for Clouds Subjected to Requests with Service Level Agreements

This paper focuses research focuses on automatic provisioning of cloud resources performed by an intermediary enterprise that provides a virtual private cloud for a single client enterprise by using resources from a public cloud. This paper concerns auto-scaling techniques for dynamically controlling the number of resources used by the client enterprise. We focus on proactive auto-scaling that is based on predictions of future workload based on the past workload. The primary goal of the auto-scaling techniques is to achieve a profit for the intermediary enterprise while maintaining a desired grade of service for the client enterprise. The technique supports both on demand requests and requests with service level agreements (SLAs). This paper presents an auto-scaling algorithm and includes a discussion of system design and implementation experience for a prototype system that implements the technique. A detailed performance analysis based on measurements made on the prototype is presented.
mTOR Complex Signaling through the SEMA4A–Plexin B2 Axis Is Required for Optimal Activation and Differentiation of CD8+ T Cells

Mammalian target of rapamycin (mTOR) plays crucial roles in activation and differentiation of diverse types of immune cells. Although several lines of evidence have demonstrated the importance of mTOR-mediated signals in CD4+ T cell responses, the involvement of mTOR in CD8+ T cell responses is not fully understood. In this study, we show that a class IV semaphorin, SEMA4A, regulates CD8+ T cell activation and differentiation through activation of mTOR complex (mTORC) 1. SEMA4A−/− CD8+ T cells exhibited impairments in production of IFN-γ and TNF-α and induction of the effector molecules granzyme B, perforin, and FAS-L. Upon infection with OVA-expressing Listeria monocytogenes, pathogen-specific effector CD8+ T cell responses were significantly impaired in SEMA4A−/− mice. Furthermore, SEMA4A−/− CD8+ T cells exhibited reduced mTORC1 activity and elevated mTORC2 activity, suggesting that SEMA4A is required for optimal activation of mTORC1 in CD8+ T cells. IFN-γ production and mTORC1 activity in SEMA4A−/− CD8+ T cells were restored by administration of recombinant Sema4A protein. In addition, we show that plexin B2 is a functional receptor of SEMA4A in CD8+ T cells. Collectively, these results not only demonstrate the role of SEMA4A in CD8+ T cells, but also reveal a novel link between a semaphorin and mTOR signaling.
A unified treatment of elastostatic contact simulation for real time haptics

We describe real-time, physically-based simulation algorithms for haptic interaction with elastic objects. Simulation of contact with elastic objects has been a challenge, due to the complexity of physically accurate simulation and the difficulty of constructing useful approximations suitable for real time interaction. We show that this challenge can be effectively solved for many applications. In particular global deformation of linear elastostatic objects can be efficiently solved with low run-time computational costs, using precomputed Green's functions and fast low-rank updates based on Capacitance Matrix Algorithms. The capacitance matrices constitute exact force response models, allowing contact forces to be computed much faster than global deformation behavior. Vertex pressure masks are introduced to support the convenient abstraction of localized scale-specific point-like contact with an elastic and/or rigid surface approximated by a polyhedral mesh. Finally, we present several examples using the CyberGlove™ and PHANToM™ haptic interfaces.
Generation of a Novel A Kinase Anchor Protein and a Myristoylated Alanine-rich C Kinase Substrate-like Analog from a Single Gene*

A unique Drosophila gene encodes two novel signaling proteins. Drosophila A kinase anchor protein 200 (DAKAP200) (753 amino acids) binds regulatory subunits of protein kinase AII (PKAII) isoforms in vitro and in intact cells. The acidic DAKAP200 polypeptide (pI ∼3.8) contains an optimal N-terminal myristoylation site and a positively charged domain that resembles the multifunctional phosphorylation site domain of vertebrate myristoylated alanine-rich C kinase substrate proteins. The 15-kilobase pair DAKAP200 gene contains six exons and encodes a second protein, ΔDAKAP200. ΔDAKAP200 is derived from DAKAP200 transcripts by excision of exon 5 (381 codons), which encodes the PKAII binding region and a Pro-rich sequence. ΔDAKAP200 appears to be a myristoylated alanine-rich C kinase substrate analog. DAKAP200 and ΔDAKAP200 are evident in vivo at all stages of Drosophiladevelopment. Thus, both proteins may play important physiological roles throughout the life span of the organism. Nevertheless, DAKAP200 gene expression is regulated. Maximal levels of DAKAP200 are detected in the pupal phase of development; ΔDAKAP200 content is elevated 7-fold in adult head (brain) relative to other body parts. Enhancement or suppression of exon 5 excision during DAKAP200 pre-mRNA processing provides potential mechanisms for regulating anchoring of PKAII and targeting of cAMP signals to effector sites in cytoskeleton and/or organelles.
One of the closest exoplanet pairs to the 3:2 mean motion resonance: K2-19b and c

Aims. The K2 mission has recently begun to discover new and diverse planetary systems. In December 2014, Campaign 1 data from the mission was released, providing high-precision photometry for similar to 22 000 objects over an 80-day timespan. We searched these data with the aim of detecting more important new objects. Methods. Our search through two separate pipelines led to the independent discovery of K2-19b and c, a two-planet system of Neptune-sized objects (4.2 and 7.2 R-circle plus), orbiting a K dwarf extremely close to the 3: 2 mean motion resonance. The two planets each show transits, sometimes simultaneously owing to their proximity to resonance and the alignment of conjunctions. Results. We obtained further ground-based photometry of the larger planet with the NITES telescope, demonstrating the presence of large transit timing variations (TTVs), and used the observed TTVs to place mass constraints on the transiting objects under the hypothesis that the objects are near but not in resonance. We then statistically validated the planets through the PASTIS tool,
Xwnt8 directly initiates expression of labial Hox genes

Hox transcription factors play an essential role in patterning the anteroposterior axis during embryogenesis and exhibit a complex array of spatial and temporal patterns of expression. Their earliest onset of expression in vertebrates is during gastrulation in a temporally collinear sequence in the presomitic/ventrolateral mesoderm, and it is not clear which upstream signal transduction events initiate this expression. Using Xenopus, we present evidence that Xwnt8 is necessary for initiation of this collinear sequence by activating Hox‐1 expression in three Hox clusters: hoxd, hoxa, and hoxb. All three labial genes appear to be direct targets of canonical Wnt signaling through Tcf/Lef. In addition, Xwnt8 loss‐ and gain‐of‐function leads to indirect regulation of other Hox genes: Hoxb4, Hoxd4, Hoxa7, Hoxc6, and Hoxc8. These findings shed new light on the early role of Wnt8 as well as of a proposed WNT gradient in patterning the Xenopus central nervous system (Kiecker and Niehrs [ 2001 ] Development 128:4189–4201). Developmental Dynamics 239:126–139, 2010. © 2009 Wiley‐Liss, Inc.
Unc-45 Mutations in Caenorhabditis elegans Implicate a CRO1/She4p-like Domain in Myosin Assembly

The Caenorhabditis elegans unc-45 locus has been proposed to encode a protein machine for myosin assembly. The UNC-45 protein is predicted to contain an NH2-terminal domain with three tetratricopeptide repeat motifs, a unique central region, and a COOH-terminal domain homologous to CRO1 and She4p. CRO1 and She4p are fungal proteins required for the segregation of other molecules in budding, endocytosis, and septation. Three mutations that lead to temperature-sensitive (ts) alleles have been localized to conserved residues within the CRO1/She4p-like domain, and two lethal alleles were found to result from stop codon mutations in the central region that would prevent translation of the COOH-terminal domain. Electron microscopy shows that thick filament accumulation in vivo is decreased by ∼50% in the CB286 ts mutant grown at the restrictive temperature. The thick filaments that assemble have abnormal structure. Immunofluorescence and immunoelectron microscopy show that myosins A and B are scrambled, in contrast to their assembly into distinct regions at the permissive temperature and in wild type. This abnormal structure correlates with the high degree of instability of the filaments in vitro as reflected by their extremely low yields and shortened lengths upon isolation. These results implicate the UNC-45 CRO1/She4p-like region in the assembly of myosin isoforms in C. elegans and suggest a possible common mechanism for the function of this UCS (UNC-45/CRO1/She4p) protein family.