Miracle-dz/UniFAR-FTUs huggingface dataset:
A new hydrogen peroxide biosensor by using modified Carbon PasteElectrode with catalase enzyme and Mn2O3 Nanoparticles

We used of Nanoscience, biology and electrochemistry in designing a new hydrogen peroxide biosensor by using modified Carbon Paste Electrode with catalase enzyme and Mn2O3 Nanoparticles. Cyclic voltammetry method as a general method in electrochemical studies was performed using an Autolab potentiostat. A conventional threeelectrode cell was employed throughout the experiments. The produced Mn2O3 Nanoparticles was characterized by XRD, UV-visible, SEM and TEM analysis. The direct electrochemistry behavior of CAT / Mn2O3 Nps/ CPE was studied and a pair of well-defined redox peaks was observed at the CAT / Mn2O3 Nps/ CPE in a 0.1 mol L−1 phosphate buffer solution (PBS) of pH 7.0 at a scan rate of 100mVs−1 compared to the Mn2O3 Nps/ CPE. Its anodic peak potential and cathodic peak potential were located at −150mV and −200mV (vs. SCE), respectively. The formal potential (Eo) of Catalase, estimated as the midpoint of reduction and oxidation potentials, was - (175 ± 2) mV. The presented experiment has introduced a new biosensor for the sensitive determination of H2O2 in the solution in the range of 15-135 μM.
Scalable distributed transactions across heterogeneous stores

Typical cloud computing systems provide highly scalable and fault-tolerant data stores that may sacrifice other features like general multi-item transaction support. Recently techniques to implement multi-item transactions in these types of systems have focused on transactions across homogeneous data stores. Since applications access data in heterogeneous storage systems for legacy or interoperability reasons, we propose an approach that enables multi-item transactions with snapshot isolation across multiple heterogeneous data stores using only a minimal set of commonly implemented features such as single item consistency, conditional updates, and the ability to store additional meta-data. We define an client-coordinated transaction commitment protocol that does not rely on a central coordinating infrastructure. The application can take advantage of the scalability and fault-tolerance characteristics of modern key-value stores and access existing data in them, and also have multi-item transactional access guarantees with little performance impact. We have implemented our design in a Java library called Cherry Garcia (CG), that supports data store abstractions to Windows Azure Storage (WAS), Google Cloud Storage (GCS) and our own high-performance key-value store called Tora.
The Mechanistic Underpinnings of an ago1-Mediated, Environmentally Dependent, and Stochastic Phenotype1[OPEN]

Perturbation of AGO1, a key enzyme in microRNA metabolism, sensitizes seedlings to stochastic instances of cell death due to up-regulated jasmonate signaling. The crucial role of microRNAs in plant development is exceedingly well supported; their importance in environmental robustness is studied in less detail. Here, we describe a novel, environmentally dependent phenotype in hypomorphic argonaute1 (ago1) mutants and uncover its mechanistic underpinnings in Arabidopsis (Arabidopsis thaliana). AGO1 is a key player in microRNA-mediated gene regulation. We observed transparent lesions on embryonic leaves of ago1 mutant seedlings. These lesions increased in frequency in full-spectrum light. Notably, the lesion phenotype was most environmentally responsive in ago1-27 mutants. This allele is thought to primarily affect translational repression, which has been linked with the response to environmental perturbation. Using several lines of evidence, we found that these lesions represent dead and dying tissues due to an aberrant hypersensitive response. Although all three canonical defense hormone pathways (salicylic acid, jasmonate, and jasmonate/ethylene pathways) were up-regulated in ago1 mutants, we demonstrate that jasmonate perception drives the lesion phenotype. Double mutants of ago1 and coronatine insensitive1, the jasmonate receptor, showed greatly decreased frequency of affected seedlings. The chaperone HEAT SHOCK PROTEIN 90 (HSP90), which maintains phenotypic robustness in the face of environmental perturbations, is known to facilitate AGO1 function. HSP90 perturbation has been shown previously to up-regulate jasmonate signaling and to increase plant resistance to herbivory. Although single HSP90 mutants showed subtly elevated levels of lesions, double mutant analysis disagreed with a simple epistatic model for HSP90 and AGO1 interaction; rather, both appeared to act nonadditively in producing lesions. In summary, our study identifies AGO1 as a major, largely HSP90-independent, factor in providing environmental robustness to plants.
Use of prasugrel in the setting of clopidogrel hypersensitivity: Case report and systematic review of the literature

Abstract Allergic reactions to clopidogrel soon after coronary stent implantation pose an important and challenging clinical problem. We describe a 44-year-old man who developed a diffuse maculopapular rash four days after initiation of clopidogrel with drug-eluting coronary stent placement. An initial treat-through strategy was unsuccessful due to patient intolerance to corticosteroids. Because of persistent hypersensitivity, clopidogrel was substituted with prasugrel which was continued successfully for one year without reaction. A systematic review of the literature was performed which identified 10 prior case reports of patients with clopidogrel hypersensitivity who were subsequently treated with prasugrel. Patient characteristics and clinical outcomes of these patients plus the current case were reviewed. There were 9 men and 2 women with ages from 44 to 76 years. All patients had undergone coronary stent procedures. Prasugrel was successfully used without cross-reactivity in 9 of the 11 patients (82%). Cross-reactivity was reported in two patients who developed hypersensitivity reactions to prasugrel similar to those experienced on clopidogrel. In conclusion, prasugrel can be used successfully in most patients with a history of clopidogrel hypersensitivity. However, potential cross-reactivity between these two thienopyridines may occur in some patients.
A REVIEW OF VISION BASED HAND GESTURES RECOGNITION

With the ever-increasing diffusion of computers into the society, it is widely believed that present popular mode of interactio ns with computers (mouse and keyboard) will become a bottleneck in the effective utilization of information flow between the computers and the human. Vision based Gesture recognition has the potential to be a natural and powerful tool supporting efficient and intuitive interaction between the human and the computer. Visual interpretation of hand gestures can help in achieving the ease and naturalness desired for Human Computer Interaction (HCI). This has motivated many researchers in computer vision-based analysis and interpretation of hand gestures as a very active research area. We surveyed the literature on visual interpretation of hand gestures in the context of its role in HCI and various seminal works of researchers are emphasized. The purpose of this review is to introduce the field of gesture recognition as a mechanism for interaction with computers.
Leading a small business? No, it's a rugby club!

Purpose – Community rugby clubs are now becoming small businesses, which are mostly led by volunteers. This paper aims to document a case study on the Leadership Academy developed by the Rugby Football Union to improve the effectiveness of community rugby club leaders.Design/methodology/approach – The programme content is explained, and written evaluations and observations analysed to assess the effectiveness of the Leadership Academy as a tool in helping to develop the community rugby club leaders.Findings – The results showed that the Leadership Academy helped the leaders to build stronger local relationships with other rugby clubs and to improve confidence levels. These outcomes are also factors that help small business owners become more effective as leaders.Originality/value – The findings from this paper provide useful input for any other organisations or governing bodies that want to improve the effectiveness of their current or future leaders.
Introduction to the Special Section on Rorschach Trauma Assessment

Bien que les etres humains aient subi et soufferts des traumatismes a toutes les epoques et en tous lieux, l’etude scientifique a grande echelle des personnes traumatisees n’a ete entamee que dans les deux dernieres decennies. Notre connaissance et comprehension des besoins des personnes traumatisees se sont certainement accrues a la faveur du changement de perspective introduit par la litterature actuelle sur le theme du traumatisme (voir par exemple Herman, 1992; van der Kolk, McFarlane & Weisaeth, 1997). Les donnees du Rorschach peuvent etre interpretees dans des perspectives differentes. Les recherches actuelles sur le traumatisme aident a nous faire mieux comprendre les reponses au Rorschach des personnes traumatisees. En retour, les donnees du Rorschach dans les cas de traumatisme peuvent enrichir notre comprehension theorique et clinique de la nature des etats post traumatiques. Les specialistes du Rorschach du monde entier se sont mis a evaluer des personnes traumatisees depuis de nombreuses annee.
On-Treatment Blood Pressure and Cardiovascular Outcomes in Older Adults With Isolated Systolic Hypertension

Our aim was to assess optimal on-treatment blood pressure (BP) at which cardiovascular disease (CVD) and all-cause mortality risks are minimized in Japanese older adults with isolated systolic hypertension. We used data from the VALISH study (Valsartan in Elderly Isolated Systolic Hypertension) that recruited older adults (n=3035; mean age, 76 years) with systolic BP (SBP) of ≥160 mm Hg and diastolic BP of <90 mm Hg. Patients were treated by valsartan. Patients were also categorized into 3 groups based on achieved on-treatment SBP of <130 mm Hg (n=317), 130 to <145 mm Hg (n=2025), or ≥145 mm Hg (n=693). The primary outcome was composite CVD (coronary heart disease, stroke, heart failure, cardiovascular deaths, other vascular diseases, and kidney diseases) with secondary outcome being all-cause mortality. Cox proportional hazards models were used to assess the CVD risk for each group. Over a median 3-year follow-up (8022 person-years), 93 CVD events and 52 deaths occurred. Using the on-treatment SBP of 130 to <145 mm Hg as reference stratum, the multivariable-adjusted hazard ratios and 95% confidence intervals of CVD and all-cause mortality risks for those with SBP<130 mm Hg were 2.08 (1.12–3.83) and 2.09 (0.93–4.71) and for those with SBP≥145 mm Hg were 2.29 (1.44–3.62) and 2.51 (1.35–4.66), respectively. On-treatment diastolic BP yielded no relationships with CVD or all-cause mortality risk. In conclusion, among Japanese older adults with isolated systolic hypertension, SBP in the range between 130 and 144 mm Hg was associated with minimal adverse outcomes and a reduction in CVD and all-cause mortality. The BP range will need to be confirmed in randomized controlled trials. Clinical Trial Registration— URL: https://www.clinicaltrials. gov. Unique identifier: NCT00151229.
4D image reconstruction for emission tomography

An overview of the theory of 4D image reconstruction for emission tomography is given along with a review of the current state of the art, covering both positron emission tomography and single photon emission computed tomography (SPECT). By viewing 4D image reconstruction as a matter of either linear or non-linear parameter estimation for a set of spatiotemporal functions chosen to approximately represent the radiotracer distribution, the areas of so-called ‘fully 4D’ image reconstruction and ‘direct kinetic parameter estimation’ are unified within a common framework. Many choices of linear and non-linear parameterization of these functions are considered (including the important case where the parameters have direct biological meaning), along with a review of the algorithms which are able to estimate these often non-linear parameters from emission tomography data. The other crucial components to image reconstruction (the objective function, the system model and the raw data format) are also covered, but in less detail due to the relatively straightforward extension from their corresponding components in conventional 3D image reconstruction. The key unifying concept is that maximum likelihood or maximum a posteriori (MAP) estimation of either linear or non-linear model parameters can be achieved in image space after carrying out a conventional expectation maximization (EM) update of the dynamic image series, using a Kullback-Leibler distance metric (comparing the modeled image values with the EM image values), to optimize the desired parameters. For MAP, an image-space penalty for regularization purposes is required. The benefits of 4D and direct reconstruction reported in the literature are reviewed, and furthermore demonstrated with simple simulation examples. It is clear that the future of reconstructing dynamic or functional emission tomography images, which often exhibit high levels of spatially correlated noise, should ideally exploit these 4D approaches.
Relationships between theory and practice in teacher education: investigating social practices in an academic discipline in a biology teaching program

In this study, we analyze processes of construction of relationships between theory and practice in teacher education. The investigation took place in a content-specific methods course in a biology-teaching program. The course was offered in a Biological Sciences Institute, addressing health teaching in elementary education. We investigated aspects of social practices in different activities of the discipline and sought to characterize the knowledge addressed. We adopted a qualitative approach with a naturalistic design. The analyses were initially based on models of teacher education discussed in the literature. Results indicate that the relationship between theory and practice varied according to the activity developed. Despite the prevalence of biological knowledge, we identified evidence of the professor's concern with educational and contextual knowledge, as well as with knowledge about students.
Formulating the Identification of Mandarin Tone2 and Tone3 in Multi-dimensional Spaces

This study examines and formulates the effect of acoustic parameters on the identification of Mandarin Tone2 and Tone3, which are timing of turning point (T_t), falling of F0 (F0_△), duration (T_d), and precursor register (Register). Three stimulus matrixes of tone tokens are set up with T_t and F0_△. Subjects identify tone tokens. In Experiment 1, matrixes are different in T_t or T_t/Td. In Experiment 2 a same target matrix is preceded by different precursor registers. Identification rate of Tone2 (P_I) is calculated. Repeated ANOVA is applied on mean P_I across each matrix. Logistic regressions between P_I and different predictors are adopted. The result shows: (1) both T_t and F0_△ are negatively relevant to P_I and interacts in a quasi-linear way; (2) both T_t and T_t/T_d, with negative effects, should be taken as independent predictors; (3) negative effect of Register is confirmed, but it works as a quadratic reciprocal predictor.
Hypertrophy of heart with outflow tract obstruction and myocardial necrosis in an infant with multiple congenital malformations.

A case of cardiac hypertrophy with obstruction of both outflow tracts, myocardial necrosis, and an apical aneurysm in a newborn infant is described. There were also congenital anomalies in the brain, kidneys, and lungs. The rarity of this particular heart anomaly in a newborn is stressed, and its possible pathogenesis discussed.
Theoretical explanations for maintenance of behaviour change: a systematic review of behaviour theories

ABSTRACT Background: Behaviour change interventions are effective in supporting individuals in achieving temporary behaviour change. Behaviour change maintenance, however, is rarely attained. The aim of this review was to identify and synthesise current theoretical explanations for behaviour change maintenance to inform future research and practice. Methods: Potentially relevant theories were identified through systematic searches of electronic databases (Ovid MEDLINE, Embase, PsycINFO). In addition, an existing database of 80 theories was searched, and 25 theory experts were consulted. Theories were included if they formulated hypotheses about behaviour change maintenance. Included theories were synthesised thematically to ascertain overarching explanations for behaviour change maintenance. Initial theoretical themes were cross-validated. Findings: One hundred and seventeen behaviour theories were identified, of which 100 met the inclusion criteria. Five overarching, interconnected themes representing theoretical explanations for behaviour change maintenance emerged. Theoretical explanations of behaviour change maintenance focus on the differential nature and role of motives, self-regulation, resources (psychological and physical), habits, and environmental and social influences from initiation to maintenance. Discussion: There are distinct patterns of theoretical explanations for behaviour change and for behaviour change maintenance. The findings from this review can guide the development and evaluation of interventions promoting maintenance of health behaviours and help in the development of an integrated theory of behaviour change maintenance.
Characterization of Adenylate Cyclase-Hemolysin Gene Duplication in a Bordetella pertussis Isolate

ABSTRACT We describe a clinical isolate of Bordetella pertussis, the agent responsible for whooping cough, composed of at least two clones harboring one or two copies of the cya locus encoding one of the major toxins, adenylate cyclase-hemolysin. No difference was observed between the two clones in murine and cellular models, probably due to the high instability of the cya locus duplication.
Measurement of self‐perception in multi‐racial context: psychometric properties of a modified Self‐Description Questionnaire

The Self-Description Questionnaire (SDQ) has been applied in a relatively large variety of cultural settings to measure the components and structure of academic and non-academic self-perception among youth. The present report examines the applicability of a modified and short form of that instrument among African American and white middle school students in the US. A modified and short form of the instrument was developed by shortening the instrument to 32 items, and extending its content to reflect self-perceptions in academic areas of science and social studies. Exploratory factor analysis and latent structural equation modelling were used to investigate the psychometric properties of the scale among racial and gender groups of middle school students. The results indicated that the factorial structure of the instrument was consistent with theoretical predictions and empirical findings across other cultures. Within both racial groups, the various subscales yielded reliability estimates typically above . 80, which is quite satisfactory for short subscales. However, additional analysis indicated that relationships among the subscales of the instrument varied considerably across racial and gender groups. It was concluded that in multi-racial and/or multicultural applications of the SDQ comparisons between groups should be done on the components (rather than overall measure) of self construct.
Studying the impact of AC-microgrid on the main grid and it's fault analysis

This paper studies the advantages of using AC-microgrids and the impact of Distributed Generators (DG's) on the main grid by studying its voltage, power flow and calculating the losses in radial as well as meshed networks. For this paper, a grid of 4 houses and 3 DG's is modelled and simulated and the results were presented and analyzed. Fault analysis is conducted on the meshed system and the inferences were drawn. It is found out that due to the integration of Distributed Generators, the power is consumed locally and hence the active and reactive power losses in the system are also minimized. Based on the simulation results conclusions were drawn.
Treatment with different doses of folic acid in haemodialysis patients: effects on folate distribution and aminothiol concentrations.

BACKGROUND Hyperhomocysteinaemia is highly prevalent among haemodialysis patients and may contribute to their increased cardiovascular risk. Treatment with pharmacological doses of folic acid lowers the plasma homocysteine concentration in these patients. The purpose of the present study was to expand the knowledge about such treatment by testing the effects of stepwise increases in the dose of folic acid on the concentrations of plasma and red blood cell folate as well as the total plasma concentrations of homocysteine (tHcy), cysteine (tCys), and glutathione (tGSH) in patients on chronic hemodialysis. METHODS Fourteen stable haemodialysis patients completed the study which consisted of four consecutive periods, each of 6 weeks duration: (i) no treatment with folic acid (control period); (ii) 5 mg of folic acid three times per week (15 mg/week); (iii) 5 mg of folic acid daily (35 mg/week); (iv) 10 mg of folic acid daily (70 mg/week). RESULTS Neither plasma or red cell folate nor plasma aminothiol concentrations changed significantly during the control period. The mean red cell folate concentration doubled during the administration of folic acid at the dose of 15 mg/week but at higher doses the further rise was only marginal. The mean folate concentration in plasma increased steeply especially at the higher doses of folic acid. During treatment with 15 mg/week of folic acid, tHcy fell by a mean of 36%, tGSH increased by a mean of 34%, but tCys was unaffected. Increases in the dose of folic acid did not augment these responses. CONCLUSIONS The maximal effect on tHcy seemed to be obtained already at the lowest given dose of folic acid (15 mg/week). At that dose, the red blood cells approached folate saturation, which may reflect the situation in other cells that participate in homocysteine metabolism and explain why further increases in the dose of folic acid are not effective from a tHcy-lowering point of view.