Miracle-dz/UniFAR-FTUs huggingface dataset:
Photostimulation of Retrotrapezoid Nucleus Phox2b-Expressing Neurons In Vivo Produces Long-Lasting Activation of Breathing in Rats

The retrotrapezoid “nucleus” (RTN), located in the rostral ventrolateral medullary reticular formation, contains a bilateral cluster of ∼1000 glutamatergic noncatecholaminergic Phox2b-expressing propriobulbar neurons that are activated by CO2 in vivo and by acidification in vitro. These cells are thought to function as central respiratory chemoreceptors, but this theory still lacks a crucial piece of evidence, namely that stimulating these particular neurons selectively in vivo increases breathing. The present study performed in anesthetized rats seeks to test whether this expectation is correct. We injected into the left RTN a lentivirus that expresses the light-activated cationic channel ChR2 (channelrhodopsin-2) (H134R mutation; fused to the fluorescent protein mCherry) under the control of the Phox2-responsive promoter PRSx8. Transgene expression was restricted to 423 ± 38 Phox2b-expressing neurons per rat consisting of noncatecholaminergic and C1 adrenergic neurons (3:2 ratio). Photostimulation delivered to the RTN region in vivo via a fiberoptic activated the CO2-sensitive neurons vigorously, produced a long-lasting (t1/2 = 11 s) increase in phrenic nerve activity, and caused a small and short-lasting cardiovascular stimulation. Selective lesions of the C1 cells eliminated the cardiovascular response but left the respiratory stimulation intact. In rats with C1 cell lesions, the mCherry-labeled axon terminals originating from the transfected noncatecholaminergic neurons were present exclusively in the lower brainstem regions that contain the respiratory pattern generator. These results provide strong evidence that the Phox2b-expressing noncatecholaminergic neurons of the RTN region function as central respiratory chemoreceptors.
Effect of liquid embolic agents on Gamma Knife surgery dosimetry for arteriovenous malformations. Clinical article.

OBJECT The effectiveness of Gamma Knife stereotactic surgery to obliterate brain arteriovenous malformations (AVMs) may be diminished by the preoperative adjunctive use of endovascular liquid embolic agents. The purpose of the present investigation was to determine if commercially available liquid embolic agents reduce the radiation dose to the target because of attenuation of the (60)Co beam. METHODS The apparent linear attenuation coefficients for 120- to 140-keV radiographs in embolized regions were retrieved from CT scans for several patients with AVMs who had undergone embolization procedures with liquid embolic agents to reduce nidal volumes. Based on these coefficients and a virtual model of Gamma Knife surgery (GKS) with basic ray tracing, the authors obtained the path lengths and densities of the embolized regions. The attenuation of (60)Co beams was then calculated for various sizes and positions of embolized AVM regions and for the number of beams used for treatment. Published experiments for several high-atomic-number materials were used to estimate the effective (60)Co beam attenuation coefficients for the N-butyl cyanoacrylate (NBCA, suspended in ethiodized oil) and ethylene vinyl alcohol copolymer (EVOH, with suspended micronized tantalum powder, Onyx) used in the AVM embolizations. Dose reductions during GKS were calculated for a theoretical model based on the CT-documented apparent linear attenuation coefficients and for the (60)Co energy attenuation coefficient. Dose measurements were obtained in a phantom study with EVOH for comparison with the estimates generated from the two attenuation coefficients. RESULTS Based on CT (keV) apparent attenuation coefficients, the authors' theoretical model predicted that the cumulative effect of either of the embolic agents decreased the number of kilovoltage photons in an embolized nidus by -8% to -15% because of the increased atomic number and density of NBCA and Onyx. However, in using the effective attenuation coefficient for the (60)Co energies as is used in GKS, the authors' theoretical model yielded only a 0.2% dose reduction per beam and a < 0.01%-0.2% dose reduction in total. These theoretical results were validated by measurements in a head phantom containing Onyx. CONCLUSIONS Dose reduction due to attenuation of the (60)Co beam by the AVM embolization material was negligible for both NBCA and EVOH because of the high-energy (60)Co beam.
Effects of processing conditions on rheological, thermal, and electrical properties of multiwall carbon nanotube/epoxy resin composites

We report on the effect of processing conditions on rheology, thermal and electrical properties of nanocomposites containing 0.02–0.3 wt % multiwall carbon nanotubes in an epoxy resin. The influence of the sonication, the surface functionalization during mixing, as well as the application of external magnetic field (EMF) throughout the curing process was examined. Rheological tests combined with optical microscopy visualization are proved as a very useful methodology to determine the optimal processing conditions for the preparation of the nanocomposites. The Raman spectra provide evidence for more pronounced effect on the functionalized with hardener compositions, particularly by curing upon application of EMF. Different chain morphology of CNTs is created depending of the preparation conditions, which induced different effects on the thermal and electrical properties of the nanocomposites. The thermal degradation peak is significantly shifted towards higher temperatures by increasing the nanotube content, this confirming that even the small amount of carbon nanotubes produces a strong barrier effect for the volatile products during the degradation. The ac conductivity measurements revealed lower values of the percolation threshold (pc) in the range of 0.03–0.05 wt %. CNTs for the nanocomposites produced by preliminary dispersing of nanotubes in the epoxy resin, compared to those prepared by preliminary functionalization of the nanotubes in the amine hardener. This is attributed to the higher viscosity and stronger interfacial interactions of the amine hardener/CNT dispersion which restricts the reorganization of the nanotubes. The application of the EMF does not influence the pc value but the dc conductivity values (σdc) of the nanocomposites increased at about one order of magnitude due to the development of the aforementioned chain structure. © 2011 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2011
Effect of Samarium Substitution on the Structural and Magnetic Properties of Nanocrystalline Cobalt Ferrite

A series of samarium-substituted cobalt ferrites ( with , 0.05, 0.10, 0.15, 0.20, 0.25) was synthesized by the sol-gel method. The structural characterizations of all the prepared samples were done using XRD and FTIR. These studies confirmed the formation of single-phase spinel structure in all the compositions. The increase in the value of lattice parameter with increase in samarium concentration suggests the expansion of unit cell. The Hall-Williamson analysis is used for estimating the average crystallite size and lattice strain induced due to the substitution of samarium in the prepared samples. Crystallinity and the crystallite size are observed to increase with the concentration of samarium. The surface morphology and particle size of a typical sample were determined using SEM and TEM respectively. The substitution of samarium strongly influences the magnetic characteristics, and this is confirmed from the magnetization measurements at room temperature.
External-memory graph algorithms

We present a collection of new techniques for designing and analyzing e cient external-memory algorithms for graph problems and illustrate how these techniques can be applied to a wide variety of speci c problems. Our results include: Proximate-neighboring. We present a simple method for deriving external-memory lower bounds via reductions from a problem we call the \proximate neighbors" problem. We use this technique to derive non-trivial lower bounds for such problems as list ranking, expression tree evaluation, and connected components. PRAM simulation. We give methods for e ciently simulating PRAM computations in external memory, even for some cases in which the PRAM algorithm is not work-optimal. We apply this to derive a number of optimal (and simple) external-memory graph algorithms. Time-forward processing. We present a general technique for evaluating circuits (or \circuit-like" computations) in external memory. We also use this in a deterministic list ranking algorithm. Department of Computer Science, Box 1910, Brown University, Providence, RI 02912{1910. y Supported in part by the National Science Foundation, by the U.S. Army Research O ce, and by the Advanced Research
Influence of Particles Type and Shape on the Corrosion Resistance of Aluminium Hybrid Composites

AMCs due to good thermal and tribological properties, they are applied as the material for: pistons in modern combustion engines, drive shafts, shock absorber cylinders and brake nodes. Heavy-duty operation, especially under tribological conditions, frequently in corrosive environment, requires knowledge on their corrosion resistance. This paper presents the initial results of the research on susceptibility of aluminium alloy matrix composite material reinforced by SiC particles and mixture of SiC+C particles to corrosion. The purpose of the research was to determine the influence of reinforcing phases, their type and shape on corrosion behaviour in a typical corrosion environment, with low NaCl concentration, in relation to the matrix alloy. Determination of corrosion resistance of Al/SiC+C hybrid composite is a new issue and falls within the field of interest of the authors of this article.
Predicting conditions for migration: effects of density dependence and habitat quality

Migration is widespread among animals, but the factors that influence the decision to migrate are poorly understood. Within a single species, populations may be completely migratory, completely sedentary or partially migratory. We use a population model to derive conditions for migration and demonstrate how migratory survival, habitat quality and density dependence on both the breeding and non-breeding grounds influence conditions for migration and the proportion of migrants within a population. Density dependence during the season in which migratory and sedentary individuals use separate sites is necessary for partial migration. High levels of density dependence at the non-shared sites widen the range of survival values within which we predict partial migration, whereas increasing the strength of density dependence at the shared sites narrows the range of survival values within which we predict partial migration. Our results have important implications for predicting how contemporary populations with variable migration strategies may respond to changes in the quality or quantity of habitat.
Association between BRAF V600E mutation and mortality in patients with papillary thyroid cancer.

IMPORTANCE BRAF V600E is a prominent oncogene in papillary thyroid cancer (PTC), but its role in PTC-related patient mortality has not been established. OBJECTIVE To investigate the relationship between BRAF V600E mutation and PTC-related mortality. DESIGN, SETTING, AND PARTICIPANTS Retrospective study of 1849 patients (1411 women and 438 men) with a median age of 46 years (interquartile range, 34-58 years) and an overall median follow-up time of 33 months (interquartile range, 13-67 months) after initial treatment at 13 centers in 7 countries between 1978 and 2011. MAIN OUTCOMES AND MEASURES Patient deaths specifically caused by PTC. RESULTS Overall, mortality was 5.3% (45/845; 95% CI, 3.9%-7.1%) vs 1.1% (11/1004; 95% CI, 0.5%-2.0%) (P < .001) in BRAF V600E-positive vs mutation-negative patients. Deaths per 1000 person-years in the analysis of all PTC were 12.87 (95% CI, 9.61-17.24) vs 2.52 (95% CI, 1.40-4.55) in BRAF V600E-positive vs mutation-negative patients; the hazard ratio (HR) was 2.66 (95% CI, 1.30-5.43) after adjustment for age at diagnosis, sex, and medical center. Deaths per 1000 person-years in the analysis of the conventional variant of PTC were 11.80 (95% CI, 8.39-16.60) vs 2.25 (95% CI, 1.01-5.00) in BRAF V600E-positive vs mutation-negative patients; the adjusted HR was 3.53 (95% CI, 1.25-9.98). When lymph node metastasis, extrathyroidal invasion, and distant metastasis were also included in the model, the association of BRAF V600E with mortality for all PTC was no longer significant (HR, 1.21; 95% CI, 0.53-2.76). A higher BRAF V600E-associated patient mortality was also observed in several clinicopathological subcategories, but statistical significance was lost with adjustment for patient age, sex, and medical center. For example, in patients with lymph node metastasis, the deaths per 1000 person-years were 26.26 (95% CI, 19.18-35.94) vs 5.93 (95% CI, 2.96-11.86) in BRAF V600E-positive vs mutation-negative patients (unadjusted HR, 4.43 [95% CI, 2.06-9.51]; adjusted HR, 1.46 [95% CI, 0.62-3.47]). In patients with distant tumor metastasis, deaths per 1000 person-years were 87.72 (95% CI, 62.68-122.77) vs 32.28 (95% CI, 16.14-64.55) in BRAF V600E-positive vs mutation-negative patients (unadjusted HR, 2.63 [95% CI, 1.21-5.72]; adjusted HR, 0.84 [95% CI, 0.27-2.62]). CONCLUSIONS AND RELEVANCE In this retrospective multicenter study, the presence of the BRAF V600E mutation was significantly associated with increased cancer-related mortality among patients with PTC. Because overall mortality in PTC is low and the association was not independent of tumor features, how to use BRAF V600E to manage mortality risk in patients with PTC is unclear. These findings support further investigation of the prognostic and therapeutic implications of BRAF V600E status in PTC.
Thermal or electrical conduction through a granular material

The material under investigation consists of particles of relatively large conductivity embedded or immersed in a matrix, the volume fraction of the particles being so high that they are in, or nearly in, contact. The particles are arranged randomly, and the material is statistically homogeneous. A general formula gives the effective conductivity of the material in terms of the average thermal (or electrical) dipole strength of a particle. The thermal flux across the surface of a particle is concentrated in areas near points of contact with another particle, and the dipole strength is approximately equal to a weighted sum of the fluxes across the areas near contact points. It is thus necessary to calculate the flux between two adjoining particles at different temperatures, and we do this by solving numerically an integral equation for the distribution of temperature over the (locally spherical) surface of one of the particles near the contact point. The flux between the two particles is found to be proportional to loge ah when a2 2h/a≫ 1 and to loge a when a 2h/a≪ 1, where h is the minimum gap between the particle surfaces, a~1 the mean of their local curvatures, and a the ratio of the conductivities of the particles and the matrix. In the case of two particles pressed together to form a circular flat spot of radius p, the flux occurs almost wholly in the particle material, and is proportional to p when ap/a≫ 1. Explicit approximate results are obtained for the effective conductivity of the granular material in the case of uniform spherical particles. For a close-packed bed of particles making point contact the effective conductivity is found to be 4.0k logea where k is the matrix conductivity. This asymptotic relation (applicable when a ≫ 1) is seen to be consistent with the available measurements of the conductivity of packed beds of spheres. Values of the effective conductivity for packed beds of particles of different shape are not expected to be greatly different.
Molecular and Immunological Evaluation of the Transcription Factor SOX-4 as a Lung Tumor Vaccine Antigen

The developmental transcription factor SOX-4 has been shown to be highly and differentially overexpressed in primary small cell lung carcinomas (SCLC). To examine the potential of SOX-4 for broad use as a lung cancer vaccine, we have evaluated the expression of SOX-4 in a panel of primary adenocarcinoma, squamous, and large cell tumor samples as well as in a panel of established small cell and non-small cell lung carcinoma tumor cell lines. SOX-4 mRNA is shown to be overexpressed in a substantial fraction of each of these lung tumor types. To examine the immunological potential of SOX-4, we have evaluated the presence of SOX-4-specific CD4 and CD8 T cells in PBMC of healthy donors and the presence of SOX4-specific Abs in sera from SCLC patients. We demonstrate the presence of both CD4 and CD8 T cells that recognize naturally processed epitopes derived from SOX-4 as well as the presence of SOX-4-specific Abs in sera from SCLC patients, but not in sera from healthy donors. The lung tumor-specific overexpression and demonstration of a comprehensive Ag-specific immune response specific for SOX-4 support the use of this molecule in the development of whole gene-, peptide-, or protein-based vaccination strategies against lung cancer. Furthermore, the identification of naturally processed T cell and Ab epitopes from SOX-4 provides valuable tools for the development of peptide-based vaccination strategies against lung cancer as well as to monitor SOX-4-specific responses in vaccinated patients.
Role of tie molecules in the yielding deformation of isotactic polypropylene

We examined the effects of the tie-molecule fraction on the yielding behavior of two isotactic polypropylenes, one having little ethylene content and the other as the homopolymer with no ethylene. The tie-molecule fraction of the samples used in this study was controlled by blending ethylene-α-olefin of an α-olefin content above 50 mol % in the blend of which the copolymers were incorporated into the amorphous regions of polypropylene (PP). An excellent linear relationship was observed between the measured yield stress and the tie-molecule fraction estimated from the Huang–Brown model, suggesting that the tie-molecule fraction and lamellar stiffness determine whether the lamellar fragmentation is easily activated or not, depending on the PP composition. Furthermore, an extended Huang–Brown model predicts a lamellar cluster connecting about five lamellae, which has a potential to account for morphological transformation of the spherulitic structure into a fibrillar one. Comparing the immiscible blends showing a phase-separated morphology with the partially miscible blends mentioned above, the yield stress was lowered by the presence of rubber phase, apparently in a similar manner; but the yielding processes were clearly discriminated between both cases when the yield stress was plotted against the tie-molecule fraction. © 1999 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 37: 357–368, 1999
Refresher Course Exercise , cognitive function , and aging

Barnes JN. Exercise, cognitive function, and aging. Adv Physiol Educ 39: 55–62, 2015; doi:10.1152/advan.00101. 2014. —Increasing the lifespan of a population is often a marker of a country’s success. With the percentage of the population over 65 yr of age expanding, managing the health and independence of this population is an ongoing concern. Advancing age is associated with a decrease in cognitive function that ultimately affects quality of life. Understanding potential adverse effects of aging on brain blood flow and cognition may help to determine effective strategies to mitigate these effects on the population. Exercise may be one strategy to prevent or delay cognitive decline. This review describes how aging is associated with cardiovascular disease risks, vascular dysfunction, and increasing Alzheimer’s disease pathology. It will also discuss the possible effects of aging on cerebral vascular physiology, cerebral perfusion, and brain atrophy rates. Clinically, these changes will present as reduced cognitive function, neurodegeneration, and the onset of dementia. Regular exercise has been shown to improve cognitive function, and we hypothesize that this occurs through beneficial adaptations in vascular physiology and improved neurovascular coupling. This review highlights the potential interactions and ideas of how the age-associated variables may affect cognition and may be moderated by regular exercise.
A software pipelining algorithm in high-level synthesis for FPGA architectures

In this paper, we present a variation of the Modulo Scheduling algorithm to exploit software pipelining in the high-level synthesis for FPGA architectures. We demonstrate the difficulties of implementing software pipelining for FPGA architectures, and propose a modified version of Modulo Scheduling that utilizes memory lifetime holes and addresses circular dependencies. Experimental results demonstrate a 35% improvement on average over the non-pipelined implementation, and 15% improvement on average over the traditional Modulo Scheduling algorithm.
Nutrient Availability Regulates SIRT1 Through a Forkhead-Dependent Pathway

Nutrient availability regulates life-span in a wide range of organisms. We demonstrate that in mammalian cells, acute nutrient withdrawal simultaneously augments expression of the SIRT1 deacetylase and activates the Forkhead transcription factor Foxo3a. Knockdown of Foxo3a expression inhibited the starvation-induced increase in SIRT1 expression. Stimulation of SIRT1 transcription by Foxo3a was mediated through two p53 binding sites present in the SIRT1 promoter, and a nutrient-sensitive physical interaction was observed between Foxo3a and p53. SIRT1 expression was not induced in starved p53-deficient mice. Thus, in mammalian cells, p53, Foxo3a, and SIRT1, three proteins separately implicated in aging, constitute a nutrient-sensing pathway.
A patient with acute exacerbation of COPD who did not respond to conventional treatment.

The patient was treated with antibiotics, oral prednisolone, and bronchodilators. He improved only with regard to his purulent cough. After 1 week of treatment, he continued to experience episodic nocturnal breathlessness. Clinical examination showed a respiratory rate of 28 breaths per minute, a pulse rate of 88 beats per minute, BP of 90/60 mm Hg, and temperature, 36.8°C. Examination of the lungs showed decreased breath sounds bilaterally without crepitations. The chest radiograph showed no new abnormalities. Arterial blood gas analysis with the patient breathing room air showed pH 7.43; PaCO2, 36 mm Hg; and PaO2, 51 mm Hg.
Polycystin-2 is a novel cation channel implicated in defective intracellular Ca(2+) homeostasis in polycystic kidney disease.

Mutations in polycystins-1 and -2 (PC1 and PC2) cause autosomal dominant polycystic kidney disease (ADPKD), which is characterized by progressive development of epithelial renal cysts, ultimately leading to renal failure. The functions of these polycystins remain elusive. Here we show that PC2 is a Ca(2+)-permeable cation channel with properties distinct from any known intracellular channels. Its kinetic behavior is characterized by frequent transitions between closed and open states over a wide voltage range. The activity of the PC2 channel is transiently increased by elevating cytosolic Ca(2+). Given the predominant endoplasmic reticulum (ER) location of PC2 and its unresponsiveness to the known modulators of mediating Ca(2+) release from the ER, inositol-trisphosphate (IP(3)) and ryanodine, these results suggest that PC2 represents a novel type of channel with properties distinct from those of the other Ca(2+)-release channels. Our data also show that the PC2 channel can be translocated to the plasma membranes by defined chemical chaperones and proteasome modulators, suggesting that in vivo, it may also function in the plasma membrane under specific conditions. The sensitivity of the PC2 channel to changes of intracellular Ca(2+) concentration is deficient in a mutant found in ADPKD patients. The dysfunction of such mutants may result in defective coupling of PC2 to intracellular Ca(2+) homeostasis associated with the pathogenesis of ADPKD.