Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of protein intake and physical activity on 24-h pattern and rate of macronutrient utilization.

Effects of moderate physical activity (90 min at 45-50% of maximal O2 uptake 2 times daily) and "high" (2.5 g protein ⋅ kg-1 ⋅ day-1, n = 6) or "normal" protein intake (1.0 g protein ⋅ kg-1 ⋅ day-1, n = 8) on the pattern and rate of 24-h macronutrient utilization in healthy adult men were compared after a diet-exercise-adjustment period of 6 days. Energy turnover (ET) was determined by indirect and direct (suit) calorimetry, and "protein oxidation" was determined by a 24-h continuous intravenous infusion of [1-13C]leucine. Subjects were in slight positive energy balance during both studies. Protein contributed to a higher (22 vs. 10%) and carbohydrate (CHO) a lower (33 vs. 58%) proportion of total 24-h ET on the high- vs. normal-protein intake. The highest contribution of fat to ET was seen postexercise during fasting (73 and 61% of ET for high and normal, respectively). With the high-protein diet the subjects were in a positive protein ( P < 0.001) and CHO balance ( P < 0.05) and a negative fat balance ( P < 0.05). The increased ET postexercise was not explained by increased rates of urea production and/or protein synthesis.
Cerebellar vascular and synaptic responses in normal mice and in transgenics with Purkinje cell dysfunction.

We used transgenic mice with Purkinje cell dysfunction (PO3 line) to study the role of these neurons in the increase in cerebellar blood flow (BFcrb) produced by stimulation of the cerebellar parallel fibers (PF). Mice (age 8-10 wk) were anesthetized (halothane) and artificially ventilated. Arterial pressure and end-tidal CO2 were monitored continuously. Arterial blood gases were measured. The PF were stimulated electrically (100 μA, 30 Hz; 40 s), and the increases in BFcrb were monitored by a laser-Doppler flow probe. First, we characterized the increases in BFcrb and the field potentials produced by PF stimulation in normal mice. PF stimulation evoked the typical field potentials and increased BFcrb by 60 ± 4% (100 μA, 30 Hz; n = 10). The increases in BFcrb were attenuated by the broad-spectrum glutamate receptor antagonist kynurenate (-84 ± 3%; P < 0.05 analysis of variance; n = 5), by thedl-α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor antagonist 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (-62 ± 6%; P < 0.05; n = 5), and by the nitric oxide synthase inhibitor N ω-nitro-l-arginine (-46 ± 7%; P < 0.05; n = 5). In PO3 transgenic mice, the increases in BFcrb produced by PF stimulation were reduced ( P < 0.001) at every stimulus intensity and frequency tested (residual increase at 100 μA, 30 Hz: 19 ± 2%; n = 6). The field potentials evoked by PF stimulation also were abnormal in that they lacked the late negative wave ( n = 6), a finding consistent with lack of depolarization of Purkinje cells. The residual flow response in the transgenics was abolished by N ω-nitro-l-arginine ( n = 5; P > 0.05). Ultrastructural studies showed that the density of PF-Purkinje cell synapses is reduced in PO3 mice, whereas the morphology of molecular layer interneurons (stellate cells) is normal. The findings suggest that Purkinje cells are responsible for a sizable component of the flow response whereas molecular layer interneurons mediate the remainder of the response. The study provides evidence that mouse mutants with spontaneous or genetically engineered cerebellar abnormalities could be useful to study the cellular and molecular correlates of functional hyperemia in the central nervous system.
Supplemental Figure 1

Supplemental Figure 1. Human iPS cells derivation and characterization. A) Morphology of human keratinocytes grown in serum-free medium. (B) Typical example of a human iPS line derived after 7 days from the mechanical picking. (C) iPS colonies (panel B) show strong alkaline phosphatase activity typical of human iPS cells. (D) Typical Embryoid Bodies (EBs) derived from human iPS cells cultured in suspension in absence of FGF. E-F) Immunofluorescence of the pluripotent transcription factors OCT4 and NANOG and of pluripotent surface antigens TRA-1-60, SSEA3, SSEA4, and TRA-1-81.G) Immunoflorescence experiments using IgG Isotype control for mouse, rabbit and rat antibodies reveals very low fluorescence background. H-J). iPS cells were specifically directed to differentiate in vitro into the three germ layers derivatives: (H) mesoderm (actinin-positive cardiac myocytes, red), (I) ectoderm (TuJ1-positive neuronal cells, green), and (J) endoderm (α-fetoprotein-positive, green). K-M). In vivo differentiation into all three germ layers through teratoma formation. (K) Histology clearly identified rivulets of muscle cells (mesoderm), L) neuro-epithelium rosettes (ectoderm) and (M) respiratory epithelium structures (endoderm). N) Expression levels of endogenous pluripotent genes quantified by qRT-PCR and plotted relative to GAPDH expression. Error bars represent standard deviation.
Update on Senecavirus Infection in Pigs

Senecavirus A (SVA) is a positive-sense single-stranded RNA virus that belongs to the Senecavirus genus within the Picornaviridae family. The virus has been silently circulating in pig herds of the USA since 1988. However, cases of senecavirus-associated vesicular disease were reported in Canada in 2007 and in the USA in 2012. Since late 2014 and early 2015, an increasing number of senecavirus outbreaks have been reported in pigs in different producing categories, with this virus being detected in Brazil, China, and Thailand. Considering the novel available data on senecavirus infection and disease, 2015 may be a divisor in the epidemiology of the virus. Among the aspects that reinforce this hypothesis are the geographical distribution of the virus, the affected pig-producing categories, clinical signs associated with the infection, and disease severity. This review presents the current knowledge regarding the senecavirus infection and disease, especially in the last two years. Senecavirus epidemiology, pathogenic potential, host immunological response, diagnosis, and prophylaxis and control measures are addressed. Perspectives are focused on the need for complete evolutionary, epidemiological and pathogenic data and the capability for an immediate diagnosis of senecavirus infection. The health risks inherent in the swine industry cannot be neglected.
Constructing argument graphs with deductive arguments: a tutorial

A deductive argument is a pair where the first item is a set of premises, the second item is a claim, and the premises entail the claim. This can be formalised by assuming a logical language for the premises and the claim, and logical entailment (or consequence relation) for showing that the claim follows from the premises. Examples of logics that can be used include classical logic, modal logic, description logic, temporal logic, and conditional logic. A counterargument for an argument A is an argument B where the claim of B contradicts the premises of A. Different choices of logic, and different choices for the precise definitions of argument and counterargument, give us a range of possibilities for formalising deductive argumentation. Further options are available to us for choosing the arguments and counterarguments we put into an argument graph. If we are to construct an argument graph based on the arguments that can be constructed from a knowledgebase, then we can be exhaustive in including all argu.
A multilevel examination of sleep, depression, and quality of life in people living with HIV/AIDS

Sleep problems are prevalent in people living with HIV/AIDS; however, few studies examine how poor sleep affects mental health and quality of life longitudinally. A sample of people living with HIV/AIDS from a randomized trial (N = 240; mean age = 47.18; standard deviation = 8.3; 71.4% male; 61.2% White) completed measures of depression (Montgomery–Åsberg Depression Rating Scale), health-related quality of life (AIDS Clinical Trial Group Quality of Life Measure), and life satisfaction (Quality of Life Inventory) at baseline and 4, 8, and 12 months. Controlling for time, condition, and relevant interactions, sleep problems significantly predicted worse outcomes over time (ps < 0.001). Findings have implications for the importance of identifying and treating sleep problems in people living with HIV/AIDS to improve mental health and quality-of-life outcomes.
Merging Physical and Digital White Canvas to Unleash Children's Creativity

This paper describes the activities and results of a workshop designed for children to understand the use of a novel interface, a hybrid physical-digital book, and at the same time to assess the kind of content that children would create. In this interface a physical book is synchronized together with a tablet computer so that flipping the page changes the content on the screen. We challenged children to create and draw a story for both the physical page and the screen. The task involved imagining a story, thinking and reflecting about it, discussing it with a peer, take decisions, develop a storyboard, and finally bring it to life on the physical book as well as on the tablet device. After the presentation of the task by the workshop organizers, children were given all the material they needed to concretize the proposed task and developed the content all by themselves. In the whole thirteen children participated in the workshop authoring five hybrid digital-physical books. In the scope of the activity children took over multiple roles, becoming authors, directors, scripters, performers and narrators, thus creating multiple layers of interaction, confirming that involving in the creation of graphic-narratives is a powerful task.
Predictive Factors of the Effectiveness of Caudal Epidural Steroid Injections in Managing Patients With Chronic Low Back Pain and Radiculopathy

Study Design: Retrospective clinical outcome analysis. Objective: To evaluate and determine whether demographic, comorbid factors, or physical examination findings may predict the outcome of caudal epidural steroid injections in managing patients with chronic low back pain and radiculopathy Summary of Background Data: The caudal epidural approach is commonly utilized with patients who are on anticoagulation or who have had prior lumbar surgery to treat L5 or S1 radiculopathies. Methods: A retrospective review of 136 patients undergoing an initial caudal epidural steroid injection for radiculopathy from January 1, 2006 to August 30, 2013. The patients were assessed before their injections for their pain levels: visual analog scale, presence of lumbar paraspinal and sciatic notch sensitivity, pain with provocative maneuvers, motor weakness, and sensory loss. The patients were then reassessed following their injection for their visual analog scale pain levels, percentage improvement, and duration of pain relief. Results: Stepwise regression was used to determine whether demographic, comorbid factors, or physical examination signs were predictive of percentage improvement or length of relief following an injection. Among these variables, duration of symptoms was found to be negatively significantly related with a P-value of 0.032 for percentage of improvement. For each week of the duration of symptoms, the percentage of improvement decreased by 0.07%. Regarding physical examination findings, presence of pain with lumbar extension was negatively and significantly related to length of relief duration with a P-value of 0.0124. The mean length of relief duration is 38.37 weeks for individuals without painful lumbar extension and 14.68 weeks for individuals with painful lumbar extension Conclusions: The mean length of relief following a caudal injection is reduced by 62% in patients who exhibit pain with lumbar extension.
Prospective study of neoadjuvant chemoradiotherapy using intensity-modulated radiotherapy and 5 fluorouracil for locally advanced rectal cancer – toxicities and response assessment

Aims and objectives The past 2 decades witnessed the strengthening of evidence favoring the role of neoadjuvant chemoradiation (CHRT) in the treatment of locally advanced rectal cancer. The study aims to evaluate the response and acute toxicities to neoadjuvant CHRT using intensity-modulated radiotherapy (IMRT) in the treatment of rectal cancer. Predictive factors to achieve pathological complete response (pCR) were analyzed, as a secondary endpoint. Materials and methods All consecutive patients who underwent IMRT as part of neoadjuvant CHRT in the treatment of rectal cancer between August 2014 and December 2016 at a tertiary cancer care center were accrued for the study. The cohort underwent CHRT with IMRT technique at a dose of 50.4 Gy in 28 fractions concurrent with continuous infusion of 5 fluorouracil during the first and the last 4 days of CHRT. Surgery was performed 6 weeks later and the pathological response to CHRT was noted. Results Forty-three subjects were accrued for the study. Radiation dermatitis and diarrhea were the only observed grade ≥3 acute toxicities. Sphincter preservation rate (SPR) was 43.3%. pCR was observed in 32.6%. Univariate and multivariate logistic regression showed that carcinoembryonic antigen was the only independent predictive factor to achieve pCR. Conclusion IMRT as part of neoadjuvant CHRT in the treatment of locally advanced rectal cancer is well tolerated and gives comparable results with respect to earlier studies in terms of pathological response and SPR. Further randomized controlled studies are needed to firmly state that IMRT is superior to 3-dimensional conformal radiotherapy.
Risk category system to identify pituitary adenoma patients with AIP mutations

Background Predictive tools to identify patients at risk for gene mutations related to pituitary adenomas are very helpful in clinical practice. We therefore aimed to develop and validate a reliable risk category system for aryl hydrocarbon receptor-interacting protein (AIP) mutations in patients with pituitary adenomas. Methods An international cohort of 2227 subjects were consecutively recruited between 2007 and 2016, including patients with pituitary adenomas (familial and sporadic) and their relatives. All probands (n=1429) were screened for AIP mutations, and those diagnosed with a pituitary adenoma prospectively, as part of their clinical screening (n=24), were excluded from the analysis. Univariate analysis was performed comparing patients with and without AIP mutations. Based on a multivariate logistic regression model, six potential factors were identified for the development of a risk category system, classifying the individual risk into low-risk, moderate-risk and high-risk categories. An internal cross-validation test was used to validate the system. Results 1405 patients had a pituitary tumour, of which 43% had a positive family history, 55.5% had somatotrophinomas and 81.5% presented with macroadenoma. Overall, 134 patients had an AIP mutation (9.5%). We identified four independent predictors for the presence of an AIP mutation: age of onset providing an odds ratio (OR) of 14.34 for age 0-18 years, family history (OR 10.85), growth hormone excess (OR 9.74) and large tumour size (OR 4.49). In our cohort, 71% of patients were identified as low risk (<5% risk of AIP mutation), 9.2% as moderate risk and 20% as high risk (≥20% risk). Excellent discrimination (c-statistic=0.87) and internal validation were achieved. Conclusion We propose a user-friendly risk categorisation system that can reliably group patients into high-risk, moderate-risk and low-risk groups for the presence of AIP mutations, thus providing guidance in identifying patients at high risk of carrying an AIP mutation. This risk score is based on a cohort with high prevalence of AIP mutations and should be applied cautiously in other populations.
Predator Migration Decisions, the Ideal Free Distribution, and Predator‐Prey Dynamics

The aim of the present work is to analyze the influence of optimal predator emigration decisions that can lead to the ideal free distribution (IFD) on the stability of predator‐prey systems. The assumption of optimal decisions is then relaxed to analyze the possible influence of different degrees of deviation from the IFD. The first migration rule we analyze is based on the marginal‐value theorem and assumes perfect knowledge of capture rate in the patch of residence and in the environment as a whole. When migration rates are high, this rule leads the predator population to the IFD. The results suggest that under these conditions predator migration plays no major role in the stability of the system. This is so because the systems naturally merge into a single patch. This result is independent of the particular functional response used. The other two rules we analyze take into account lower migration rates, the limitations in making optimal decisions by predators, and the possible constraints in the assessment of intake rate in the different patches. The results suggest that the processes that hinder the convergence of the populations to the IFD might make a major contribution to the stability of the system.
Herpes Simplex Virus Replication: Roles of Viral Proteins and Nucleoporins in Capsid-Nucleus Attachment

ABSTRACT Replication of herpes simplex virus type 1 (HSV-1) involves a step in which a parental capsid docks onto a host nuclear pore complex (NPC). The viral genome then translocates through the nuclear pore into the nucleoplasm, where it is transcribed and replicated to propagate infection. We investigated the roles of viral and cellular proteins in the process of capsid-nucleus attachment. Vero cells were preloaded with antibodies specific for proteins of interest and infected with HSV-1 containing a green fluorescent protein-labeled capsid, and capsids bound to the nuclear surface were quantified by fluorescence microscopy. Results showed that nuclear capsid attachment was attenuated by antibodies specific for the viral tegument protein VP1/2 (UL36 gene) but not by similar antibodies specific for UL37 (a tegument protein), the major capsid protein (VP5), or VP23 (a minor capsid protein). Similar studies with antibodies specific for nucleoporins demonstrated attenuation by antibodies specific for Nup358 but not Nup214. The role of nucleoporins was further investigated with the use of small interfering RNA (siRNA). Capsid attachment to the nucleus was attenuated in cells treated with siRNA specific for either Nup214 or Nup358 but not TPR. The results are interpreted to suggest that VP1/2 is involved in specific attachment to the NPC and/or in migration of capsids to the nuclear surface. Capsids are suggested to attach to the NPC by way of the complex of Nup358 and Nup214, with high-resolution immunofluorescence studies favoring binding to Nup358.
Discovering Macrophage Functions Using In Vivo Optical Imaging Techniques

Macrophages are an important component of host defense and inflammation and play a pivotal role in immune regulation, tissue remodeling, and metabolic regulation. Since macrophages are ubiquitous in human bodies and have versatile physiological functions, they are involved in virtually every disease, including cancer, diabetes, multiple sclerosis, and atherosclerosis. Molecular biological and histological methods have provided critical information on macrophage biology. However, many in vivo dynamic behaviors of macrophages are poorly understood and yet to be discovered. A better understanding of macrophage functions and dynamics in pathogenesis will open new opportunities for better diagnosis, prognostic assessment, and therapeutic intervention. In this article, we will review the advances in macrophage tracking and analysis with in vivo optical imaging in the context of different diseases. Moreover, this review will cover the challenges and solutions for optical imaging techniques during macrophage intravital imaging.
Can a Non-Native Primate Be a Potential Seed Disperser? A Case Study on Saimiri sciureus in Pernambuco State, Brazil

The interaction between native fleshy-fruit plants and introduced fruit consumers contributes to the dynamics of highly fragmented environments. Such interactions can occur through pollination and seed dispersal. Here, we investigated the potential of seed dispersing by a non-native primate, the squirrel monkey (Saimiri sciureus), in an urban Atlantic forest fragment in north-eastern Brazil. Fleshy fruits from non-native plants were preferentially exploited by the squirrel monkeys. We measured 147 seeds (width and length) from 20 of 106 faecal samples. The dispersed seeds were from five plant species (four families). We found a positive correlation between the size of seeds found in faecal samples with their availability in the habitat. Only one seed was found visually damaged after passage through the squirrel monkeys’ guts. The defecated seeds were uniformly distributed (variance to mean ratio index) in the home range of squirrel monkeys, and there was a lack of specific latrines (i.e., habitat preference for defecation). Our data provide evidence that non-native S. sciureus may in be (i) acting as a potential seed disperser of native plants in the Atlantic forest fragment, (ii) contributing to the ecological role of native frugivores, and (iii) potentially contributing to the regeneration process of the highly degraded study site.
Adult attachment and drinking context as predictors of alcohol problems and relationship satisfaction in college students

ABSTRACT Background: Evidence suggests that for young adults, intimate partners influence each other’s drinking patterns. Therefore, exploration of variables related to intimate partner relationships (such as attachment style) could broaden the current understanding of risk factors for alcohol problems in this demographic. Objectives: The current study examined the role of drinking context in the relationships among insecure attachment, alcohol problems, and relationship satisfaction. A path model was hypothesized where the relationship between insecure attachment and alcohol problems would be explained via two distinct drinking contexts (i.e., drinking with one’s partner and drinking away from one’s partner). It was also hypothesized that the relationship between insecure attachment and relationship satisfaction would be explained via these same two drinking contexts. Methods: Participants were 194 undergraduate students ages 18–25 who reported being in a monogamous intimate partner relationship for at least 90 days and had also consumed alcohol in the past 90 days. The sample was comprised of 76% women and 24% men. Results: The hypothesized direct relationship from anxious attachment to alcohol problems was significant; there were also significant direct paths from both anxious and avoidant attachment to relationship satisfaction. The hypotheses regarding indirect relationships were not supported. Conclusion: The results of this study contribute to the existing literature, in that they suggest that drinking in the context of an intimate relationship may not directly affect relationship satisfaction in this population. However, relationship functioning still appears to be an important variable to consider in the prevention and treatment of alcohol-related problems affecting college students.