Do GST Polymorphisms Modulate the Frequency of Chromosomal Aberrations in Healthy Subjects ?

Rossi et al. (2009) described an association between chromosomal aberration (CA) frequency and cancer risk in a case–control study on 107 cancer cases and 291 controls, whereby they observed no modifying effect of poly morphisms in glutathione S‐transferase M1 (GSTM1) and GSTT1. In our studies of 488 healthy individuals who shared the same environmental exposure in Slovakia and the Czech Republic, we observed a CA frequency of 2.35 ± 1.73 (mean ± SD) (Halasova et al. 2007; Musak et al. 2008; Naccarati et al. 2006; Slyskova et al. 2007; Vodicka et al. 2001, 2004a, 2004b). The frequencies (mean ± SD) for chromatid-type abberations (CTA) and chromosome-type aberrations (CSA) were 1.22 ± 1.21 and 1.15 ± 1.35, respectively. By analyzing modulating effects of genetic poly morphisms in GSTT1, GSTM1, and GSTP1 on CAs, CTAs, and CSAs (Table 1), we found no significant association between chromosomal damage and any of the studied polymorphisms. The results were further confirmed by logistic regression: for the GSTT1 null genotype, odds ratio (OR) = 1.35 [95% confidence interval (CI), 0.79–2.32; p = 0.27]; for the GSTM1 genotype, OR = 1.09 [95% CI, 0.74–1.62; p = 0.65]; and for a variant GSTP1 Val105Val genotype, OR = 0.83 [95% CI, 0.55–1.24; p = 0.36]. These data on a larger healthy population [previously published separately by Halasova et al. (2007), Musak et al. (2008), Naccarati et al. (2006), Slyskova et al. (2007), and Vodicka et al. (2001, 2004a, 2004b)] confirm the findings of Rossi et al. (2009) regarding GSTM1 and GSTT1 polymorphisms. Additionally, in our reanalysis, we did not observe any modulating effect of GSTP1 polymorphism on CA frequency. However, the modu lating role of GST polymorphisms may not be excluded, particularly in interaction with heavy occupational exposure. In our study exploring chromosomal damage in tire-plant workers (Musak et al. 2008), CAs were significantly higher in subjects with GSTT1-null than in those with GSTT1-plus genotypes, particularly in association with smoking. In the past decade, CAs have been accepted as a predictive marker of cancer (Hagmar et al. 2004), particularly for colorectal and lung cancers (Boffetta et al. 2007; Norppa et al. 2006). Nevertheless, these studies, as well as the study of Rossi et al. (2009) may have limitations: For example, cohorts were recruited in various regions with different life style and environmental backgrounds, and different laboratories were involved in processing and scoring the samples over many years. In earlier studies, virtually no data on individual susceptibility were available because of the lack of DNA for molecular analysis. The data on CAs presented here were obtained on healthy subjects from a homogeneous region with fairly similar socioeconomic background. The analysis of CAs reported in these studies (Halasova et al. 2007; Musak et al. 2008; Naccarati et al. 2006; Slyskova et al. 2007; Vodicka et al. 2001, 2004a, 2004b) were performed in two laboratories, using the same protocol and the same scoring criteria with regular slide exchanges to minimize inter laboratory and inter scorer differences. Also, native DNA from wholeblood samples for molecular genetic studies was collected simultaneously with the samples for cyto genetic investigations. Future prospective studies regarding CAs and cancer should be designed by taking into account the life style and occupational/ environmental exposures, along with factors of individual susceptibility. Some GST polymorphisms may modulate CA frequency through inter action with environmental factors. The next logical step for a confirmation of predictive values of CA frequencies in relation to cancer will be their determination in lympho cytes of cancer patients in association with clinical– pathological characteristics. The authors declare they have no compet‐ ing financial interests.