Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of fisetin as a potential inducer of mitochondrial biogenesis in SH-SY5Y neuronal cells

Objective(s): Increasing evidence implicates impaired mitochondrial biogenesis as an important contributor to mitochondrial dysfunction, which plays a central role in the pathogenesis of neurodegenerative diseases including Parkinson’s disease (PD). For this reason, targeting mitochondrial biogenesis may present a promising therapeutic strategy for PD. The present study attempted to investigate the effects of fisetin, a dietary flavonoid, on mitochondrial biogenesis and the expression of PD-associated genes in neuronal cells. Materials and Methods: The effects of fisetin on mitochondrial biogenesis were evaluated by three different approaches; PGC-1α and TFAM mRNA expressions by RT-qPCR, mitochondrial DNA (mtDNA) content by quantitative PCR and mitochondrial mass by MitoTracker staining. Next, a PCR array was performed to evaluate the effects of fisetin on the expression profile of PD-associated genes. Finally, the common targets of fisetin and PD were analyzed by in silico analyses. Results: The results demonstrated that fisetin treatment can increase PGC-1α and TFAM mRNA levels, mtDNA copy number, and mitochondrial mass in neuronal cells. Fisetin also altered the expressions of some PD-related genes involved in neuroprotection and neuronal differentiation. Moreover, the bioinformatics analyses suggested that the AKT1-GSK3B signaling might be responsible for the potential neuroprotective effects of fisetin. Conclusion: Collectively, these results imply that fisetin could modulate some neuroprotective mechanisms including mitochondrial biogenesis, and may serve as a potential drug candidate for PD.
Atopic dermatitis and cognitive dysfunction in middle-aged and older adults: A systematic review and meta-analysis

Background Atopic dermatitis (AD) is a common chronic inflammatory skin disease that affects adults worldwide. Recent evidence suggests that AD may be associated with cognitive dysfunction, but the results of individual studies have been inconsistent. This systematic review and meta-analysis aimed to evaluate the association between AD and cognitive dysfunction in middle-aged and older adults. Methods To find relevant research, a comprehensive search of electronic databases from the beginning to March 2023 was carried out. Data were taken from studies that were eligible, and a meta-analysis was done to determine the pooled hazard ratio (HR) and 95% confidence interval (CI). Results We searched three databases and found a total of 15 studied arms included in 5 cohort studies with over 8.5 million participants were included in the analysis. The results showed that individuals with AD had a higher risk of developing dementia of all-cause dementia (pooled hazard ratio (HR) = 1.16; 95% CI, 1.10–1.23,P<0.001) and the Alzheimer type (pooled HR = 1.28; 95% CI, 1.01–1.63,P<0.001) but not vascular dementia (pooled HR = 1.42; 95% CI, 0.99–2.04,P<0.001). Subgroup analyses showed that the association between atopic dermatitis and all-cause dementia was significant in Europe (P = 0.004) but not in Asia (P = 0.173) and was significant in prospective cohort studies (P<0.001) but not in non-prospective cohort studies (P = 0.068). Sensitivity analysis and publication bias detection confirmed the reliability of the overall findings. Conclusions In conclusion, this study demonstrated that AD was associated with increased risk of cognitive dysfunction, particularly dementia of the Alzheimer type and all-cause dementia, in middle-aged and older participants. Further research is needed to understand the mechanisms behind this association and its potential implications for clinical practice. Systematic review registration PROSPERO, identifier (CRD42023411627).
Mitochondrial calcium uniporter activates TFEB-driven autophagy to promote migration of breast cancer cells

Objective(s): Tumor metastasis is the leading cause of death in breast cancer (BC) patients and is a complicated process. Mitochondrial calcium uniporter (MCU), a selective channel responsible for mitochondrial Ca2+ uptake, has been reported to be associated with tumorigenesis and metastasis. The molecular mechanisms of MCU contributing to the migration of BC cells are partially understood. This study investigated the role of MCU in BC cell metastasis and explored the underlying mechanism of MCU-mediated autophagy in BC cell migration. Materials and Methods: The Kaplan-Meier plotter database was used to analyze the prognostic value of MCU mRNA expression. Western blotting was used to examine the expression level of MCU in 4 paired BC and adjacent normal tissues. The cellular migration capability of BC was measured by transwell migration assay and wound healing assay. Western blotting and reverse transcription-quantitative polymerase chain reaction were performed to detect the expression levels of autophagy-related markers. The effects of MCU activation or inhibition on TFEB nuclear translocation in BC cells were detected by laser scanning confocal microscopy. Results: Expression of MCU was found to be negatively correlated with BC patient prognosis in the Kaplan-Meier plotter database. Compared with the adjacent normal tissues, MCU was markedly up-regulated in the BC tissues. MCU overexpression promoted cellular migration, activated autophagy, and increased TFEB nuclear translocation in BC cells, whereas its knockdown produced the opposite effects. Conclusion: MCU activates TFEB-driven autophagy to promote BC cell metastasis and provides a potential novel therapeutic target for BC clinical intervention.
Honey contamination from plant protection products approved for cocoa cultivation: a systematic review of existing research and methods

Cocoa (Theobroma cocoa), which is the key ingredient of chocolate, is an important economic crop plant which supports the livelihoods of an estimated forty to fifty million people directly involved in its cultivation. Many cocoa producing countries, especially those from the developing world, rely on the income from cocoa export to support their economies. The plant is, however, prone to disease and pest attacks and therefore requires the application of large volumes of pesticides to guarantee satisfactory productions. Even though pesticides help protect the cocoa plant from disease and pest attacks, unintended effects of environmental contamination are also a possibility. Honey, a product of nectar collected by honeybees from flowers during foraging, may be a useful proxy for the extent to which landscapes are exposed to pesticides and the degree of pesticide accumulation in the environment. The overreaching question is: to what extent has the effect of pesticides imputed for cocoa production on honey received attention in research? In this present study, we conducted a systematic approach to quantify existing studies on honey contamination from plant protection products approved for cocoa cultivation. We observed that one hundred and sixty-nine different compounds, comprising some recommended and other unapproved compounds for cocoa cultivation, were detected in 81% of the reviewed 104 publications. Our results further point to the neonicotinoids as the most detected class of pesticides, with imidacloprid particularly being the single most detected compound. However, the most remarkable observation made from this study points to disproportionate studies of honey contamination from pesticides conducted in cocoa and non-cocoa producing countries with only 19% of the publications taking place in the latter. To bridge the gap, we suggest prioritising increased research in cocoa growing countries to ameliorate the significant gaps in knowledge owing to limited studies emanating from these geographic regions.
Population Exposure Changes to Mean and Extreme Climate Events Over Pakistan and Associated Mechanisms

The increasing prevalence of warmer trends and climate extremes exacerbate the population's exposure to urban settlements. This work investigated population exposure changes to mean and extreme climate events in different Agro‐Ecological Zones (AEZs) of Pakistan and associated mechanisms (1979−2020). Spatiotemporal trends in mean and extreme temperatures revealed significant warming mainly over northern, northeastern, and southern AEZs. In contrast, mean‐to‐extreme precipitation changes showed non‐uniform patterns with a significant increase in the northeast AEZs. Population exposure to mean (extreme) temperature and precipitation events increased two‐fold during 2000–2020. The AEZs in urban settlements (i.e., Indus Delta, Northern Irrigated Plain, and Barani/Rainfall) show a maximum exposure to extreme temperatures of about 70–100 × 106 (person‐days) in the reference period (1979−1999), which increases to 140–200 × 106 person‐days in the recent period (2000−2020). In addition, the highest exposure to extreme precipitation days also increases to 40–200 × 106 person‐days during 2000–2020 than 1979−1999 (20–100 × 106) person‐days. Relative changes in exposure are large (60%–90%) for the AEZs across northeast Pakistan, justifying the spatial population patterns over these zones. Overall, the observed changes in exposure are primarily attributed to the climate effect (50%) over most AEZs except Northern Irrigated Plain for R10 and R20 events, where the interaction effect takes the lead. The population exposure rapidly increased over major AEZs of Pakistan, which could be more vulnerable to extreme events due to rapid urbanization and population growth in the near future.
Regulation of cell proliferation and transdifferentiation compensates for ventilator‐induced lung injury mediated by NLRP3 inflammasome activation

Mechanical ventilation is an important means of respiratory support and treatment for various diseases. However, its use can lead to serious complications, especially ventilator‐induced lung injury (VILI). The mechanisms underlying this disease are complex, but activation of inflammatory signalling pathways results in activation of cytokines and inflammatory mediators, which play key roles in VILI. Recent studies have demonstrated that nod‐like receptor protein 3 (NLRP3) inflammasome activation mediates VILI and also accompanied by cell proliferation and transdifferentiation to compensate for alveolar membrane damage. Type I alveolar epithelial cells (AECs I), which are involved in the formation of the blood‐air barrier, are vulnerable to damage but cannot proliferate by themselves; thus, replacing AECs I relies on type II alveolar epithelial cells (AECs II).
New Zealand GPS velocity field: 1995–2013

ABSTRACT We collate nearly two decades of campaign GPS data gathered at over 900 sites throughout New Zealand to release a New Zealand nationwide GPS velocity field. The data span the entire North and South islands of New Zealand with a typical spacing of 10–20 km and a denser network (c. 2–8 km spacing) in the Wellington region, central Taupo Volcanic Zone and parts of the Arthur's Pass area. The dataset provides the most comprehensive-to-date view of crustal deformation within the Australia–Pacific plate boundary zone in the New Zealand region. We discuss the data acquisition, processing and derivation of the velocities and uncertainties. We also undertake corrections for earthquake displacements to obtain a velocity field that is largely representative of interseismic deformation between 1995 and 2013.
Magnetotelluric imaging of fluids in intraplate earthquake zones, NE Japan Back Arc

Intraplate earthquake zones in the back arc of NE Japan were imaged by wide‐band magnetotelluric (MT) soundings. The 90km long MT profile of 34 stations extends over the two topographic features, the Dewa Hills and the Ou Backbone Range, which were uplifted by thrust faults. MT data show two‐dimensionality and strong TE/TM anisotropic responses at the periods around 100 s. After tensor decompositions with regional strike of N12°E, two‐dimensional inversion was carried out where static shift was also a model parameter. The final model is characterized by conductive blocks in the mid‐crust to account for the anisotropic responses. Correlation of the conductors to the seismic scatterers and to the low velocity anomalies suggests that the conductors represent fluids. High seismicity clustering near the rims of conductors suggests that the intraplate seismicity results either from the migration of the fluids to less permeable crust or from local stress concentration near the structural boundary.
Effects of Ramadan Fasting on Extracorporeal Photopheresis Outcomes

Although there are many studies on the impact of Ramadan fasting on health in the medical literature, the effects have not been explored in Muslim patients undergoing extracorporeal photopheresis (ECP). This report aimed to describe the potential effects of Ramadan fasting on ECP treatment outcomes. Patients undergoing ECP were prospectively evaluated before and during the month of Ramadan 1443 AH (2022 AD) at the Abu Dhabi Stem Cells Center (ADSCC), United Arab Emirates. The following ECP outcomes were assessed: treatment completion, adverse events reported, body mass index (BMI), and laboratory test results, including complete blood count (CBC), C-reactive protein (CRP), and other systemic immune-inflammatory biomarkers (SIIBs). No statistically significant differences were found in most of the variables analyzed in the three patients who underwent ECP before and during the holy month. Two non-fasting patients were not able to complete the Ramadan ECP schedule, and one fasting patient experienced a vascular access event during his first procedure in Ramadan. These findings suggest that fasting during Ramadan could add further risk factors and develop serious complications related to the ECP treatment. Therefore, we suggest that fasting should be avoided during photopheresis treatment, and we provided recommendations to achieve the best possible clinical outcomes.
Characterization of pyridylpiperazine-based efflux pump inhibitors for Acinetobacter baumannii

Abstract Objectives In Acinetobacter baumannii, multidrug efflux pumps belonging to the resistance-nodulation-division (RND) superfamily result in decreased antibiotic susceptibility. Improving the activity of current antibiotics via efflux pump inhibitors (EPIs) represents an attractive alternative approach to control this bacterium. Pyridylpiperazines (PyrPips) are a new class of EPIs that can effectively inhibit the Escherichia coli RND efflux pump AcrAB-TolC and boost the activity of several antibiotics. Here we have evaluated and characterized whether the PyrPip chemical family is also able to boost antibiotic activity through inhibition of the RND efflux pumps in A. baumannii. Methods Comparative structural modelling and docking, structure-activity relationship studies alongside molecular genetic approaches were deployed to improve, characterize and validate PyrPips’ target. Results We showed that two enhanced PyrPip EPIs are capable of rescuing the activity of different classes of antibiotics in A. baumannii. By expressing A. baumannii main efflux pumps (AdeB, AdeG and AdeJ) individually in E. coli recombinant strains, we could gain further insights about the EPIs’ capacity to act upon each pump. Finally, we showed that PyrPip EPIs are mostly acting through AdeJ inhibition via interactions with two key charged residues, namely E959 and E963. Conclusions Our work demonstrates that PyrPip EPIs are capable of inhibiting RND efflux pumps of A. baumannii, and thus may present a promising chemical scaffold for further development.
Plant-based red colouration of shell beads 15,000 years ago in Kebara Cave, Mount Carmel (Israel)

Decorating the living space, objects, body and clothes with colour is a widespread human practice. While the habitual use of red mineral pigments (such as iron-oxide, e.g., ochre) by anatomically modern humans started in Africa about 140,000 years ago, the earliest documentation of the use of organic plant or animal-based red pigments is known from only 6,000 years ago. Here, we report the oldest reliable evidence of organic red pigment use 15,000 years ago by the first sedentary hunter-gatherers in the Levant. SEM-EDS and Raman Spectroscopy analyses of 10 red-stained shell beads enabled us to detect and describe the use of a colourant made of Rubiaceae plants roots (Rubia spp., Asperula spp., Gallium spp.) to colour personal adornments from the Early Natufian of Kebara cave, Mount Carmel, Israel. This adds a previously unknown behavioural aspect of Natufian societies, namely a well-established tradition of non-dietary plant processing at the beginning of the sedentary lifestyle. Through a combined multidisciplinary approach, our study broadens the perspectives on the ornamental practices and the chaînes opératoires of pigmenting materials during a crucial period in human history.
Retention of zirconia crown to oxidized titanium base abutment: Experimental research

Statement of problem To separate the crown from the titanium base abutment, by using heat, caused oxidization of the titanium base abutment. The effect of this procedure on the retention of a crown is unclear. Purpose To compare the resin bond strength and failure type between zirconia crowns and titanium base abutments utilizing four different surface treatments. Surface roughness and morphology of each surface treatment were also investigated. Material and methods Forty titanium base abutments (Variobase®) were divided into four groups, 1. Control, 2. Air abraded, 3. Oxidized, and 4. Oxidized-air abraded. Oxidized and oxidized-air abraded groups were debonded from zirconia crowns using constant dry heat at 500 ˚C. For air abraded and oxidized-air abraded groups (after oxidization), the titanium base abutments were air abraded with Al3O2. After cleaning, one specimen of each group was investigated under a non-contact profilometer (50x), then the same samples were investigated under SEM at 25,300,500,1000 magnification and EDS at 30kV of accelerated voltage. All specimens were then cemented (RelyX Ultimate). After aging, with thermocycling under 5C° to 55C°,120 seconds dwell time for 5,000 cycles, bond strength was tested and statistical differences were calculated with One-way ANOVA (p-value <0.05) follow by Tukey test. All separated crowns and titanium base abutments were investigated under a light microscope (20x), using fisher’s exact test for correlation of the failure types. Results There was a significant difference in the mean value of tensile bond strength among the control and test groups. Comparisons between control(237.6±46.3N) and oxidized(241.7±46.3N) showed statistically different values from air abraded(372.9±113.2N) when assembled using different surface treatments of the titanium-based abutments. (p-value<0.005) As for failure type, there were statistically significant differences between control versus air abraded, control versus oxidized-air abraded, oxidized versus air abraded, and oxidized versus oxidized-air abraded. (p-value<0.001) The titanium surface morphology shown from the profilometer and SEM was coordinated. Control (Ra 333.8nm) and oxidized (Ra 321.0nm) groups surfaces showed smooth, corrugated surfaces, meanwhile air abraded (Ra 476.0nm) and oxidized-air abraded (Ra 423.8nm) groups showed rough, rugged surfaces. Conclusion Heat oxidization of titanium-based abutments did not adversely affect tensile bond strength or the failure mode and surface roughness between titanium base abutments and zirconia crowns. However, air abrasion of the titanium surface increased surface roughness and retentive strength. Clinical implications The titanium base abutments that were oxidized under heat treatment did not have an effect on crown retention. Thoroughly air abraded the titanium abutment prior to cementation can increase cement bond strength.
A multifunctional soft robot for cardiac interventions

In minimally invasive endovascular procedures, surgeons rely on catheters with low dexterity and high aspect ratios to reach an anatomical target. However, the environment inside the beating heart presents a combination of challenges unique to few anatomic locations, making it difficult for interventional tools to maneuver dexterously and apply substantial forces on an intracardiac target. We demonstrate a millimeter-scale soft robotic platform that can deploy and self-stabilize at the entrance to the heart, and guide existing interventional tools toward a target site. In two exemplar intracardiac procedures within the right atrium, the robotic platform provides enough dexterity to reach multiple anatomical targets, enough stability to maintain constant contact on motile targets, and enough mechanical leverage to generate newton-level forces. Because the device addresses ongoing challenges in minimally invasive intracardiac intervention, it may enable the further development of catheter-based interventions.
Ocean Radiant Heating. Part I: Optical Influences

Abstract Radiative transfer calculations are used to quantify the effects of physical and biological processes on variations in the transmission of solar radiation through the upper ocean. Results indicate that net irradiance at 10 cm and 5 m can vary by 23 and 34 W m−2, respectively, due to changes in the chlorophyll concentration, cloud amount, and solar zenith angle (when normalized to a climatological surface irradiance of 200 W m−2). Chlorophyll influences solar attenuation in the visible wavebands, and thus has little effect on transmission within the uppermost meter where the quantity of near-infrared energy is substantial. Beneath the top few meters, a chlorophyll increase from 0.03 to 0.3 mg m−3 can result in a solar flux decrease of more than 10 W m−2. Clouds alter the spectral composition of the incident irradiance by preferentially attenuating in the near-infrared region, and serve to increase solar transmission in the upper few meters as a greater portion of the irradiance exists in the deep-.
Suspended sediment load transport in the Mississippi River basin at St. Louis: temporal scaling and nonlinear determinism

This study investigates the dynamic behavior of suspended sediment load transport at different temporal scales in the Mississippi River basin. Data corresponding to five successively doubled temporal scales (i.e. daily, two‐day, four‐day, eight‐day and 16‐day) from the St. Louis gaging station in Missouri are analyzed. The investigation is focused on identifying possible low‐dimensional deterministic behavior in the suspended sediment load transport dynamics, with an aim towards reduction in model complexity. The correlation dimension method is used to identify low‐dimensional determinism. The suspended sediment load dynamics are represented through phase‐space reconstruction, and the variability is estimated using the (proximity of) reconstructed vectors in the phase space. The results indicate the presence of low‐dimensional determinism in the suspended sediment load series at each of the five temporal scales, with the variables dominantly governing the dynamics in the order of three or four. These results not only suggest the appropriateness of relatively simpler models but also hint at possible scale invariance in the suspended sediment load transport dynamics. Copyright © 2006 John Wiley & Sons, Ltd.