Miracle-dz/UniFAR-FTUs huggingface dataset:
Utopia: A load sharing facility for large, heterogeneous distributed computer systems

Load sharing in large, heterogeneous distributed systems allows users to access vast amounts of computing resources scattered around the system and may provide substantial performance improvements to applications. We discuss the design and implementation issues in Utopia, a load sharing facility specifically built for large and heterogeneous systems. The system has no restriction on the types of tasks that can be remotely executed, involves few application changes and no operating system change, supports a high degree of transparency for remote task execution, and incurs low overhead. The algorithms for managing resource load information and task placement take advantage of the clustering nature of large‐scale distributed systems; centralized algorithms are used within host clusters, and directed graph algorithms are used among the clusters to make Utopia scalable to thousands of hosts. Task placements in Utopia exploit the heterogeneous hosts and consider varying resource demands of the tasks. A range of mechanisms for remote execution is available in Utopia that provides varying degrees of transparency and efficiency.
Dynamic measurement of the pH of the Golgi complex in living cells using retrograde transport of the verotoxin receptor

The B subunit of verotoxin (VT1B) from enterohemorrhagic Escherichia coli is responsible for the attachment of the holotoxin to the cell surface, by binding to the glycolipid, globotriaosyl ceramide. After receptor-mediated endocytosis, the toxin is targeted to the Golgi complex by a process of retrograde transport. We took advantage of this unique property of VT1B to measure the pH of the Golgi complex in intact live cells. Purified recombinant VT1B was labeled with either rhodamine or fluorescein for subcellular localization by confocal microscopy. After 1 h at 37 degrees C, VT1B accumulated in a juxtanuclear structure that colocalized with several Golgi markers, including alpha-mannosidase II, beta-COP, and NBD-ceramide. Moreover, colchicine and brefeldin A induced dispersal of the juxtanuclear staining, consistent with accumulation of VT1B in the Golgi complex. Imaging of the emission of fluorescein-labeled VT1B was used to measure intra-Golgi pH (pHG), which was calibrated in situ with ionophores. In intact Vero cells, pHG averaged 6.45 +/- 0.03 (standard error). The acidity of the Golgi lumen dissipated rapidly upon addition of bafilomycin A1, a blocker of vacuolar-type ATPases, pHG remained constant despite acidification of the cytosol by reversal of the plasmalemmal Na+/H+ antiport. Similarly, pHG was unaffected by acute changes in cytosolic calcium. Furthermore, pHG recovered quickly toward the basal level after departures imposed with weak bases. These findings suggest that pHG is actively regulated, despite the presence of a sizable H+ "leak" pathway. The ability of VT1B to target the Golgi complex should facilitate not only studies of acid-base regulation, but also analysis of other ionic species.
Company TRACKING CONTROL OF COMPLEMENTARITY LAGRANGIAN SYSTEMS *

In this paper we study the tracking control of Lagrangian systems subject to frictionless unilateral constraints. The stability analysis incorporates the hybrid and nonsmooth dynamical feature of the overall system. The difference between tracking control for unconstrained systems and unilaterally constrained ones, is explained in terms of closed-loop desired trajectories and control signals. This work provides details on the conditions of existence of controllers which guarantee stability. It is shown that the design of a suitable transition phase desired trajectory, is a crucial step. Some simulation results provide information on the robustness aspects. Finally the extension towards the case of multiple impacts, is considered.
Kohn-Sham potentials and wave functions from electron densities.

A method is introduced that allows the determination of the Kohn-Sham effective potential and the corresponding Kohn-Sham orbitals belonging to an arbitrary electron density with boundary conditions of a bound and finite system. For atoms or molecules the exchange-correlation potential and the exchange-correlation energy can be obtained also. The procedure easily can be implemented using existing density-functional programs
Analysis of rodent platelet glycoprotein IIb: evidence for evolutionarily conserved domains and alternative proteolytic processing.

Recently, the full-length primary amino acid sequence for human glycoproteins (GP) IIb and IIIa have been derived from their respective cDNAs. Potential functional domains within these proteins have been proposed based primarily on homology with similar domains in other proteins having known biologic function. To further understand the relationship between structure and function of the platelet fibrinogen receptor, we have begun comparative studies of the human GPIIb/IIIa receptor with the corresponding rodent receptor. The rodent rGPIIb/IIIa receptor differs from the human receptor, having low affinity for R.G. D-containing oligopeptides and not binding at all to the C-terminus of the gamma chain of human fibrinogen. We describe the structure of rodent platelet GPIIb derived from a combination of peptide sequencing, and cDNA and partial genomic DNA sequence analysis. The initial transcript is 1037 amino acid residues, having 78% amino acid identity with its 1039 residue human analog. Both heavy chains have the N-terminal sequence L.N. L.D, agreeing with the consensus derived from other integrin family alpha heavy chains. All 18 cysteine residues occur at positions conserved in human GPIIb and the vitronectin receptor alpha subunit VNR alpha. The putative calcium-binding domains of the GPIIbs have a high level of amino acid identity (92%), supporting the supposition that these regions have a critical biologic role. The final 48 C-terminal amino acid residues of the heavy chain of rodent GPIIb share only 56% identity with its human counterpart, and the proposed cleavage site of human GPIIb into its heavy and light chains is not present in the rodent sequence. Although we demonstrate that rodent GPIIb is split into two subunits during its maturation, this process either involves a different recognition sequence in the rodent or occurs at a different site. Finally, partial genomic DNA sequence analysis indicates that there are at least two rodent GPIIb genes: a normal gene, containing introns in positions similar to those in the human gene, and a processed pseudogene. The human haploid genome contains only a single GPIIb gene.
Inflammation, Oxidative Stress, and Repair Capacity of the Vascular Endothelium in Obstructive Sleep Apnea

Background— Indirect evidence implicates endothelial dysfunction in the pathogenesis of vascular diseases associated with obstructive sleep apnea (OSA). We investigated directly whether dysfunction and inflammation occur in vivo in the vascular endothelium of patients with OSA. The effects of continuous positive airway pressure (CPAP) therapy on endothelial function and repair capacity were assessed. Methods and Results— Thirty-two patients with newly diagnosed OSA and 15 control subjects were studied. Proteins that regulate basal endothelial nitric oxide (NO) production (endothelial NO synthase [eNOS] and phosphorylated eNOS) and inflammation (cyclooxygenase-2 and inducible NOS) and markers of oxidative stress (nitrotyrosine) were quantified by immunofluorescence in freshly harvested venous endothelial cells before and after 4 weeks of CPAP therapy. Vascular reactivity was measured by flow-mediated dilation. Circulating endothelial progenitor cell levels were quantified to assess endothelial repair capacity. Baseline endothelial expression of eNOS and phosphorylated eNOS was reduced by 59% and 94%, respectively, in patients with OSA compared with control subjects. Expression of both nitrotyrosine and cyclooxygenase-2 was 5-fold greater in patients with OSA than in control subjects, whereas inducible NOS expression was 56% greater. Expression of eNOS and phosphorylated eNOS significantly increased, whereas expression of nitrotyrosine, cyclooxygenase-2, and inducible NOS significantly decreased in patients who adhered to CPAP ≥4 hours daily. Baseline flow-mediated dilation and endothelial progenitor cell levels were lower in patients than in control subjects, and both significantly increased in patients who adhered to CPAP ≥4 hours daily. Conclusions— OSA directly affects the vascular endothelium by promoting inflammation and oxidative stress while decreasing NO availability and repair capacity. Effective CPAP therapy is associated with the reversal of these alterations.
Hard deadlines and Dual Priority Scheduling

The problem of effectively scheduling soft tasks whilst guaranteeing the behaviour of hard tasks has been addressed in many papers and a large number of techniques have been proposed. The dual priority mechanism is an intuitively simple method with low overheads. A hard task is assigned two priorities. Upon invocation, the task starts executing with a low priority and it is promoted to a high priority at a time that will guarantee that its deadline is met. Soft tasks are assigned medium priorities; they can thus preempt any hard task that is executing before its promotion time. To increase the capacity for soft tasks, and therefore the effectiveness of the real-time system, hard tasks may be assigned a n m -hard (read n inm) temporal constraint. This implies that the task must meet n deadlines in any m invocations. This paper addresses the combination of such constraints and dual priority scheduling. This approach reduces the gap between dynamic priority and fixed priority scheduling with the goal of reducing the average response time of soft tasks.
Multi-Objective Planning of Multi-Type Distributed Generation Considering Timing Characteristics and Environmental Benefits

This paper presents a novel approach to multi-type distributed generation (DG) planning based on the analysis of investment and income brought by grid-connected DG. Firstly, the timing characteristics of loads and DG outputs, as well as the environmental benefits of DG are analyzed. Then, on the basis of the classification of daily load sequences, the typical daily load sequence and the typical daily output sequence of DG per unit capacity can be computed. The proposed planning model takes the location, capacity and types of DG into account as optimization variables. An improved adaptive genetic algorithm is proposed to solve the model. Case studies have been carried out on the IEEE 14-node distribution system to verify the feasibility and effectiveness of the proposed method and model.
PARK7 and nucleoside diphosphate kinase A as plasma markers for the early diagnosis of stroke.

BACKGROUND Plasma markers for stroke could be useful in diagnosis and prognosis and in prediction of response of stroke patients to therapy. PARK7 and nucleoside diphosphate kinase A (NDKA) are increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. METHODS We used ELISA to measure PARK7 and NDKA in plasma in 3 independent European and North American retrospective studies encompassing a total of 622 stroke patients and 165 control individuals. RESULTS Increases in both biomarkers were highly significant, with sensitivities of 54%-91% for PARK7 and 70%-90% for NDKA and specificities of 80%-97% for PARK7 and 90%-97% for NDKA. The concentrations of both biomarkers increased within 3 h of stroke onset. CONCLUSIONS PARK7 and NDKA may be useful plasma biomarkers for the early diagnosis of stroke. In addition, this study demonstrated the utility of analysis of postmortem CSF proteins as a first step in the discovery of plasma markers of ischemic brain injury.
Time of Day and Nutrients in Feeding Govern Daily Expression Rhythms of the Gene for Sterol Regulatory Element-binding Protein (SREBP)-1 in the Mouse Liver*

Sterol regulatory element-binding protein-1 (SREBP-1) plays a central role in transcriptional regulation of genes for hepatic lipid synthesis that utilizes diet-derived nutrients such as carbohydrates and amino acids, and expression of SREBP-1 exhibits daily rhythms with a peak in the nocturnal feeding period under standard housing conditions of mice. Here, we report that the Srebp-1 expression rhythm shows time cue-independent and Clock mutation-sensitive circadian nature, and is synchronized with varied photoperiods apparently through entrainment of locomotor activity and food intake. Fasting caused diminution of Srebp-1 expression, while diabetic db/db and ob/ob mice showed constantly high expression with loss of rhythmicity. Time-restricted feedings during mid-light and mid-dark periods exhibited differential effects, the latter causing more severe damping of the oscillation. Therefore, “when to eat in a day (the light/dark cycle),” rather than “whenever to eat in a day,” is a critical determinant to shape the daily rhythm of Srebp-1 expression. We further found that a high-carbohydrate diet and a high-protein diet, as well as a high-fat diet, cause phase shifts of the oscillation peak into the light period, underlining the importance of “what to eat. ” Daily rhythms of SREBP-1 protein levels and Akt phosphorylation levels also exhibited nutrient-responsive changes. Taken together, these findings provide a model for mechanisms by which time of day and nutrients in feeding shape daily rhythms of the Srebp-1 expression and possibly a number of other physiological functions with interindividual and interdaily differences in human beings and wild animals subjected to day-by-day changes in dietary timing and nutrients.
Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

Health effects associated with particulate matter (PM) show seasonal variations. We hypothesized that these heterogeneous effects may be attributed partly to the differences in the elemental composition of PM. Normal human bronchial epithelial (NHBE) cells and alveolar macrophages (AMs) were exposed to equal mass of coarse [PM with aerodynamic diameter of 2.5–10 μm (PM2.5–10)], fine (PM2.5), and ultrafine (PM < 0.1) ambient PM from Chapel Hill, North Carolina, during October 2001 (fall) and January (winter), April (spring), and July (summer) 2002. Production of interleukin (IL)-8, IL-6, and reactive oxygen species (ROS) was measured. Coarse PM was more potent in inducing cytokines, but not ROSs, than was fine or ultrafine PM. In AMs, the October coarse PM was the most potent stimulator for IL-6 release, whereas the July PM consistently stimulated the highest ROS production measured by dichlorofluorescein acetate and dihydrorhodamine 123 (DHR). In NHBE cells, the January and the October PM were consistently the strongest stimulators for IL-8 and ROS, respectively. The July PM increased only ROS measured by DHR. PM had minimal effects on chemiluminescence. Principal-component analysis on elemental constituents of PM of all size fractions identified two factors, Cr/Al/Si/Ti/Fe/Cu and Zn/As/V/Ni/Pb/Se, with only the first factor correlating with IL-6/IL-8 release. Among the elements in the first factor, Fe and Si correlated with IL-6 release, whereas Cr correlated with IL-8 release. These positive correlations were confirmed in additional experiments with PM from all 12 months. These results indicate that elemental constituents of PM may in part account for the seasonal variations in PM-induced adverse health effects related to lung inflammation.
Investigating the effec tiveness of the intervention reading models of two teachers in grades K-2

EDUCATIONAL LEADERSHIP WILLIAMS, RACHEL A. B.A. COLUMBIA COLLEGE, 2000 M.ED. UNIVERSITY OF WEST GEORGIA, 2005 ED.S. UNIVERSITY OF WEST GEORGIA, 2007 INVESTIGATING THE EFFECTIVENESS OF THE INTERVENTION READING MODELS OF TWO TEACHERS IN GRADES K-2 Advisor: Dr. Moses Norman Dissertation dated May 2013 This study examines the effectiveness of two reading interventionists and their teaching methodologies with students in grades K-2. The two interventionists were selected because they are the two teachers responsible for reading intervention in the primary grades. The students were selected because they are being served by the interventionists and they are performing below proficient in reading. Many students come into Title I schools underperforming for a variety of reasons. These include a lack of literacy resources in their homes and also a lack of outside experiences. Many parents in this school setting are working poor. They hold jobs, but do not have a much time to spend with their child due to making ends meet financially. A case study approach was used to gather data. The researcher conducted three observations on each teacher for a total of six observations. All three grades levels (K-2) were observed in a pull out setting. The observer utilized an observation instrument and also an interview protocol to interview both teachers. Additionally, student achievement was analyzed using DIBELS Reading 3D data. The data was collected at the beginning of the year and the end of the year and compared to measure student reading growth. The researcher found that both teachers regardless of age, race, and experience were effective at raising student achievement with at risk students. There were no significant differences in the achievement between males and females, between students who received free and reduced lunch and those who did not, or among ethnicities. First grade students however made significantly higher gains than the other two grade levels in this study. Both teachers showed 100% growth according to Reading 3D scores. Additionally, they agreed that given autonomy and time to plan and build trust with regular education teachers they were more successful. They believe in the importance of accountability and providing supports to underachieving students. The conclusions drawn from the findings suggest that various teaching methodologies which include differentiation, a focus on the big five components of reading, and small teacher to student ratios were successful. Strong connections with students were seen from each teacher as they both knew their children and their strengths and weaknesses. This research suggests that given full autonomy to deliver instruction without a scripted program, both teachers were highly effective. Districts should use teachers, such as the ones in this study, to conduct professional development trainings on best practices in literacy. Leaders are encouraged to give teachers more autonomy in their classrooms. Intervention should be considered in higher grade levels to bridge gaps in reading.
Estimation of global leaf area index and absorbed par using radiative transfer models

A simple method for the estimation of global leaf area index (LAI) and fraction of photosynthetically active radiation absorbed by the vegetation (FAPAR) from atmospherically corrected Normalized Difference Vegetation Index (NDVI) observations is described. Recent improvements to the authors' three dimensional radiative transfer model of a vegetated surface are described. Example simulation results and a validation exercise are discussed. The model was utilized to derive land cover specific NDVI-LAI and NDVI-FAPAR relations. The method therefore requires stratification of global vegetation into cover types that are compatible with the radiative transfer model. Such a classification based on vegetation structure is proposed and a simple method for its derivation is presented. Proof-of-concept results are given to illustrate the feasibility of the proposed method.
Association of two resistance mechanisms in a clinical isolate of Enterobacter cloacae with high-level resistance to imipenem

Carbapenem resistance was studied in a clinical isolate of Enterobacter cloacae, strain 201 (MIC of imipenem and meropenem, 16 micrograms/ml). This strain was analyzed comparatively with the carbapenem-susceptible parent strain 200, an equally susceptible revertant, 201-Rev, and in vitro-selected mutants with different levels of carbapenem resistance. All strains produced similarly high amounts of the same cephalosporinase (pIapp = 8.8). Strain 201 apparently lacked two major outer membrane proteins of ca. 37 and 38 kDa, while 201-Rev produced only the 37-kDa protein. The permeability coefficient, determined with cephaloridine, was reduced up to ninefold in the resistant strains which also showed a substantial reduction in the uptake of [14C]meropenem. The introduction of the plasmid-borne ampD gene (whose product decreases the expression of ampC) resulted in almost complete cessation of cephalosporinase production in all strains and a substantial decrease in the MICs of the carbapenems which remained, however, 8- to 16-fold higher than those determined for the susceptible strains containing the ampD gene. This "residual" resistance was attributed to reduced outer membrane permeability. The contribution of cephalosporinase production was verified in a reverse experiment, in which the introduction of ampC into a low-level cephalosporinase producer resulted in a fourfold increase in the carbapenem MICs. From these results, we infer that reduced outer membrane permeability and high-level cephalosporinase production can operate in conjunction in clinical isolates of E. cloacae to confer imipenem resistance.
Blind, Greedy, and Random: Ordinal Approximation Algorithms for Matching and Clustering

We study Matching and other related problems in a partial information setting where the agents' utilities for being matched to other agents are hidden and the mechanism only has access to ordinal preference information. Our model is motivated by the fact that in many settings, agents cannot express the numerical values of their utility for different outcomes, but are still able to rank the outcomes in their order of preference. Specifically, we study problems where the ground truth exists in the form of a weighted graph, and look to design algorithms that approximate the true optimum matching using only the preference orderings for each agent (induced by the hidden weights) as input. If no restrictions are placed on the weights, then one cannot hope to do better than the simple greedy algorithm, which yields a half optimal matching. Perhaps surprisingly, we show that by imposing a little structure on the weights, we can improve upon the trivial algorithm significantly: we design a 1.6-approximation algorithm for instances where the hidden weights obey the metric inequality. Using our algorithms for matching as a black-box, we also design new approximation algorithms for other closely related problems: these include a a 3.2-approximation for the problem of clustering agents into equal sized partitions, a 4-approximation algorithm for Densest k-subgraph, and a 2.14-approximation algorithm for Max TSP. These results are the first non-trivial ordinal approximation algorithms for such problems, and indicate that we can design robust algorithms even when we are agnostic to the precise agent utilities.