Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of Microbial Adherence to Polymethylmethacrylate for Maxillo-Facial Prostheses

2 Abstract: This study aimed to compare the adhesion of three microorganisms on Polymethylmethacrylate materials surfaces used in maxillo-facial prostheses. Candida albicans, Streptococcus mutans and Staphylococcus aureus were incubated separately with Polymethylmethacrylate (PMMA) (n = 18) for 30 days at 37 °C. The counts of viable microorganisms in the accumulating biofilm layer were determined and converted to colony-forming units per cm unit surface area. Scanning electron microscope (SEM) was used to evaluate 2 the microbial adhesion. Statistical analysis was performed using one-way ANOVA. Significant differences in microbial adhesion were observed on PMMA after the cells were incubated for 30 days (p < 0.001). SEM showed evident of microbial adhesion on PMMA surface.
Novel M tuberculosis Antigen-Specific T-Cells Are Early Markers of Infection and Disease Progression

Background Mycobacterium tuberculosis Region-of-Difference-1 gene products present opportunities for specific diagnosis of M. tuberculosis infection, yet immune responses to only two gene-products, Early Secretory Antigenic Target-6 (ESAT-6) and Culture Filtrate Protein-10 (CFP-10), have been comprehensively investigated. Methods T-cell responses to Rv3873, Rv3878 and Rv3879c were quantified by IFN-γ-enzyme-linked-immunospot (ELISpot) in 846 children with recent household tuberculosis exposure and correlated with kinetics of tuberculin skin test (TST) and ESAT-6/CFP-10-ELISpot conversion over six months and clinical outcome over two years. Results Responses to Rv3873, Rv3878, and Rv3879c were present in 20–25% of contacts at enrolment. Rv3873 and Rv3879c responses were associated with and preceded TST conversion (P = 0.02 and P = 0.04 respectively), identifying these antigens as early targets of cell-mediated immunity following M. tuberculosis exposure. Responses to Rv3873 were additionally associated with subsequent ESAT-6/CFP-10-ELISpot conversion (P = 0.04). Responses to Rv3873 and Rv3878 predicted progression to active disease (adjusted incidence rate ratio [95% CI] 3.06 [1.05,8.95; P = 0.04], and 3.32 [1.14,9.71; P = 0.03], respectively). Presence of a BCG-vaccination scar was associated with a 67% (P = 0.03) relative risk reduction for progression to active tuberculosis. Conclusions These RD1-derived antigens are early targets of cellular immunity following tuberculosis exposure and T-cells specific for these antigens predict progression to active tuberculosis suggesting diagnostic and prognostic utility.
A Covert Channel Over VoLTE via Adjusting Silence Periods

Covert channels represent unforeseen communication methods that exploit authorized overt communication as the carrier medium for covert messages. Covert channels can be a secure and effective means of transmitting confidential information hidden in overt traffic. For covert timing channel, the covert message is usually modulated into inter-packet delays (IPDs) of legitimate traffic, which is not suitable for voice over LTE (VoLTE) since the IPDs of VoLTE traffic are fixed to lose the possibility of being modulated. For this reason, we propose a covert channel via adjusting silence periods, which modulates covert message by the postponing or extending silence periods in VoLTE traffic. To keep the robustness, we employ the Gray code to encode the covert message to reduce the impact of packet loss. Moreover, the proposed covert channel enables the tradeoff between the robustness and voice quality which is an important performance indicator for VoLTE. The experiment results show that the proposed covert channel is undetectable by statistical tests and outperforms the other covert channels based on IPDs in terms of robustness.
Interleaved Training and Training-Based Transmission Design for Hybrid Massive Antenna Downlink

In this paper, we study the beam-based training design jointly with the transmission design for hybrid massive antenna single-user (SU) and multiple-user (MU) systems where outage probability is adopted as the performance measure. For SU systems, we propose an interleaved training design to concatenate the feedback and training procedures, thus making the training length adaptive to the channel realization. Exact analytical expressions are derived for the average training length and the outage probability of the proposed interleaved training. For MU systems, we propose a joint design for the beam-based interleaved training, beam assignment, and MU data transmissions. Two solutions for the beam assignment are provided with different complexity-performance tradeoff. Analytical results and simulations show that for both SU and MU systems, the proposed joint training and transmission designs achieve the same outage performance as the traditional full-training scheme but with significant saving in the training overhead.
Dynamic clustering with a mobile sink in wireless sensor networks

Wireless sensor network is one of the most important tools to achieve information and understanding of the environment, which has attracted many scientific researches in recent years. Network lifetime is the most important criterion for measuring and evaluating the wireless sensor network. One of the common techniques to improve the network lifetime is clustering. This solution, despite its benefits, has caused a problem of energy hole. Therefore, in this paper a dynamic clustering method with a mobile sink is proposed to moderate the energy hole. In proposed protocol, we use an unequal clustering method and multi-objective secure algorithm to regulate energy consumption and for each cluster, we consider the vice cluster-head node. Simulation results show that our proposed method can effectively reduce energy loss, balance energy consumption and increase network lifetime.
Preliminary normative variation of auditory P300 parameters in adult individuals

Background and Aim: Auditory P300 is an event-related potential. Cognitive factors like attention are involved in the generation of P300. It seems that normative variation of P300 is necessary for clinical purposes. Thus, the current study was designed to establish preliminary normative variation of P300 amplitude and latency at Fz and Cz sites in adults. Methods: This cross-sectional study was performed on 20 right-handed volunteers aged 18 to 33 years. P300 was recorded monaurally with two channels at Fz and Cz placements. Two tone bursts of 1000Hz and 2000Hz were used as frequent and target stimuli, respectively. Results: The mean values of P300 amplitude and latency at Cz were 7.43±2.61 µv and 325.19±21.34 ms in the right ear and 7.38±2. 73µv and 320.29±21.56 ms in the left ear, respectively. At Fz, the mean values of P300 amplitude and latency were 5.34±1.74 µv and 330.09±25.58 ms in the right ear and 5.67±2.30 µv and 329.52±29.25 ms in the left ear, respectively. The differences between the ears at Cz and Fz were not statistically significant (p˃0.05). The mean value of amplitude of P300 was significantly greater at Cz than Fz (p=0.001) although the difference in latency was not statistically significant between Cz and Fz (p˃0.05). Conclusion: Amplitude of P300 was greater at Cz than Fz although latency was not different. Based on these findings, amplitude and latency values can be probably used for clinical purposes to assess auditory disorders.
Molecular identification of antelope horn by melting curve analysis

Abstract Antelope horn is a valuable Chinese traditional medicine and widely used in clinic. However, with the deterioration of antelope’s living environment and a lot of killing, the saiga population begins falling and in some places plummet. Since the increasing demand of this expensive and good bioactive medicine, the horn of artiodactyla animals is often used as the antelope horn. The adulterated or impostor not only cause damage to clinical medicine but also affect the antelope resources protection and sustainable development. Here, in order to establish a melting curve analysis (MCA) method to distinguish the antelope horn from other animal horns and identify the decoction pieces and Chinese patent medicine in a fast and easy way, animal horns and its decoction pieces, Chinese patent medicines were collected from the market and the DNA of all the collected samples were extracted. The melting curve of two universal fragments (COI and Cyt b) was scanned and Cyt b was selected as feasibility fragment for identifying authentic antelope horn from eight adulterant animal horns. After optimizing the condition for MCA, inspecting the precision and the replication of the method, a reference melting curve modern was established and we performed MCA on the antelope horns, fakes, and adulterants on a 1:1 mix, decoction pieces, and Chinese patent medicine. Thus, this study provides fast and easy methods so that MCA can detect the truth, fakes, and adulterations of antelope horns.
A 665 μW Silicon Photomultiplier-Based NIRS/EEG/EIT Monitoring ASIC for Wearable Functional Brain Imaging

This paper presents a sub-mW ASIC for multimodal brain monitoring. The ASIC is co-integrated with electrode(s) and optodes (i.e., optical source and detector) as an active sensor to measure electroencephalography (EEG), bio-impedance (BioZ), and near-infrared spectroscopy (NIRS) on scalp. The target is to build a wearable EEG-NIRS headset for low-cost functional brain imaging. The proposed NIRS readout utilizes the near-infrared light to measure the pulse oximetry and blood oxygen saturation (SpO2). While traditional photodiodes are supported, the readout also allows the use of silicon photomultipliers (SiPMs) as optical detectors. The SiPM improves optical sensitivity while significantly reducing the average power of two LEDs to 150 μW. On circuit level, a SAR-based calibration compensates maximum 40 μA current from ambient light, while digital DC-servo loops reduces the baseline static SiPM current up to 400 μA, leading to an overall dynamic range of 87 dB. The EEG readout exhibits 720 MΩ input impedance at 50 Hz. The BioZ readout has 3 mΩ/√(Hz) impedance sensitivity by employing dynamic circuit techniques. When EEG, BioZ, and NIRS are enabled at the same time, one ASIC consumes 665 μW including the power of LEDs.
Micro-social structural approaches to HIV prevention: a social ecological perspective

Abstract To be effective and sustainable, HIV-prevention interventions need to be sufficiently powerful to counteract prevailing social norms and diffuse through the targeted community to provide social reinforcement for behaviour change. Social structural and environmental factors are major influences on HIV-related behaviours yet the dearth of conceptualization and operationalization of these factors impede progress in intervention development. In this paper we propose a social ecological perspective to intervention and highlight relevant theories from social psychology and organizational behaviour literatures. We examine social networks and social settings as micro-structural and environmental influences on HIV risk behaviours, social identities and norms, and as important targets for HIV-prevention intervention. Intervention approaches are proposed that target networks and behavioural settings and provide participants with socially meaningful and rewarding behavioural options that are consistent with valued prosocial identities or roles. Examples are presented on how such an approach has been utilized in prior HIV prevention interventions, including our social network-oriented intervention that trained disadvantaged former and current illicit drug users to conduct peer outreach. We describe how behavioural interventions may enhance or introduce new prosocial identities and social roles, and that network members may confer social approval to reinforce these identities and roles, leading to sustained behavioural risk reduction and changes in risk behaviour norms.
Video transmission based on Orthogonal Frequency Division Multiplexing (OFDM) utilizing television white space

Television white space (TVWS) is an underutilized spectrum that can be one of the possible solutions for spectrum scarcity problem in wireless communication. This paper presents a development of Orthogonal Frequency Division Multiplexing (OFDM) based video transmission using software defined radio (SDR) platform in television white space. Two types of modulation schemes are used namely Phase-shift keying (PSK) and Quadrature amplitude modulation (QAM) for video transmission in an indoor environment under ultra high frequency (UHF) band. The proposed system is developed with the physical layer design of transmitter and receiver in GNU radio and integration of medium access control layer functionality. A video capture and display program are designed based on OpenCV modules. Experiment results have shown that under allocated bandwidth of 1.0 MHz, QPSK exhibits the best performance in terms of packet delivery ratio and end to end delay for slow motion video while BPSK performs better for fast and medium motion videos.
An Architecture to Accelerate Convolution in Deep Neural Networks

In the past few years, the demand for real-time hardware implementations of deep neural networks (DNNs), especially convolutional neural networks (CNNs), has dramatically increased, thanks to their excellent performance on a wide range of recognition and classification tasks. When considering real-time action recognition and video/image classification systems, latency is of paramount importance. Therefore, applications strive to maximize the accuracy while keeping the latency under a given application-specific maximum: in most cases, this threshold cannot exceed a few hundred milliseconds. Until now, the research on DNNs has mainly focused on achieving a better classification or recognition accuracy, whereas very few works in literature take in account the computational complexity of the model. In this paper, we propose an efficient computational method, which is inspired by a computational core of fully connected neural networks, to process convolutional layers of state-of-the-art deep CNNs within strict latency requirements. To this end, we implemented our method customized for VGG and VGG-based networks which have shown state-of-the-art performance on different classification/recognition data sets. The implementation results in 65-nm CMOS technology show that the proposed accelerator can process convolutional layers of VGGNet up to 9.5 times faster than state-of-the-art accelerators reported to-date while occupying 3.5 mm2.
How does temperature affect splicing events? Isoform switching of splicing factors regulates splicing of LATE ELONGATED HYPOCOTYL (LHY)

Abstract One of the ways in which plants can respond to temperature is via alternative splicing (AS). Previous work showed that temperature changes affected the splicing of several circadian clock gene transcripts. Here, we investigated the role of RNA‐binding splicing factors (SFs) in temperature‐sensitive AS of the clock gene LATE ELONGATED HYPOCOTYL (LHY). We characterized, in wild type plants, temperature‐associated isoform switching and expression patterns for SF transcripts from a high‐resolution temperature and time series RNA‐seq experiment. In addition, we employed quantitative RT‐PCR of SF mutant plants to explore the role of the SFs in cooling‐associated AS of LHY. We show that the splicing and expression of several SFs responds sufficiently, rapidly, and sensitively to temperature changes to contribute to the splicing of the 5′UTR of LHY. Moreover, the choice of splice site in LHY was altered in some SF mutants. The splicing of the 5′UTR region of LHY has characteristics of a molecular thermostat, where the ratio of transcript isoforms is sensitive to temperature changes as modest as 2 °C and is scalable over a wide dynamic range of temperature. Our work provides novel insight into SF‐mediated coupling of the perception of temperature to post‐transcriptional regulation of the clock.
Physical Characteristics and Performance of Japanese Top-Level American Football Players

Abstract Yamashita, D, Asakura, M, Ito, Y, Yamada, S, and Yamada, Y. Physical characteristics and performance of Japanese top-level American football players. J Strength Cond Res 31(9): 2455–2461, 2017—This study aimed to compare the physical characteristics and performance between top-level nonprofessional football players in Japan and National Football League (NFL) Combine invited players and between top-level and middle-level players in Japan to determine the factors that enhance performance in international and national competitions. A total of 168 American football players (>20 years) in Japan participated in an anthropometric (height and weight) and physical (vertical jump, long jump, 40-yard dash, pro-agility shuttle, 3-cone drill, and bench press repetition test) measurement program based on the NFL Combine program to compete in the selection of candidates for the Senior World Championship. All players were categorized into 1 of the 3 position groups based on playing position: skill players, big skill players, and linemen. Japanese players were additionally categorized into selected and nonselected players for the second tryout. The NFL Combine candidates had significantly better performance than selected Japanese players on all variables except on performance related to quickness among the 3 position groups. Compared with nonselected players, selected Japanese skill players had better performance in the 40-yard dash and bench press test and big skill players had better performance in the vertical jump, broad jump, and 40-yard dash. Selected and nonselected Japanese linemen were not different in any measurements. These results showed the challenges in American football in Japan, which include not only improving physical performance of top-level players, but also increasing the number of football players with good physical performance.
Mutations in MICAL‐1cause autosomal‐dominant lateral temporal epilepsy

Autosomal‐dominant lateral temporal epilepsy (ADLTE) is a genetic focal epilepsy characterized by auditory symptoms. Two genes, LGI1 and RELN, encoding secreted proteins, are implicated in the etiology of ADLTE, but half of the affected families remain genetically unsolved, and the underlying molecular mechanisms are yet to be clarified. We aimed to identify additional genes causing ADLTE to better understand the genetic basis and molecular pathway underlying this epileptic disorder.
School-Based Sleep Education Programs for Short Sleep Duration in Adolescents: A Systematic Review and Meta-Analysis.

BACKGROUND Insufficient sleep among students is a major school health problem. School-based sleep education programs tailored to reach large number of students may be one of the solutions. A systematic review and meta-analysis was conducted to summarize the programs' effectiveness and current status. METHODS Electronic databases were searched up until May 2015. Randomized controlled trials of school-based sleep intervention among 10- to 19-year-old students with outcome on total sleep duration were included. Methodological quality of the studies was assessed using the Cochrane's risk of bias assessment. RESULTS Seven studies were included, involving 1876 students receiving sleep education programs and 2483 attending classes-as-usual. Four weekly 50-minute sleep education classes were most commonly provided. Methodological quality was only moderate, with a high or an uncertain risk of bias in several domains. Compared to classes-as-usual, sleep education programs produced significantly longer weekday and weekend total sleep time and better mood among students at immediate post-treatment, but the improvements were not maintained at follow-up. CONCLUSIONS Limited by the small number of studies and methodological limitations, the preliminary data showed that school-based sleep education programs produced short-term benefits. Future studies should explore integrating sleep education with delayed school start time or other more effective approaches.
Novel Roles of cAMP Receptor Protein (CRP) in Regulation of Transport and Metabolism of Carbon Sources

CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using the newly developed Genomic SELEX screening system of transcription factor-binding sequences, however, we have identified a total of at least 254 CRP-binding sites. Based on their location on the E. coli genome, we predict a total of at least 183 novel regulation target operons, altogether with the 195 hitherto known targets, reaching to the minimum of 378 promoters as the regulation targets of cAMP-CRP. All the promoters selected from the newly identified targets and examined by using the lacZ reporter assay were found to be under the control of CRP, indicating that the Genomic SELEX screening allowed to identify the CRP targets with high accuracy. Based on the functions of novel target genes, we conclude that CRP plays a key regulatory role in the whole processes from the selective transport of carbon sources, the glycolysis-gluconeogenesis switching to the metabolisms downstream of glycolysis, including tricarboxylic acid (TCA) cycle, pyruvate dehydrogenase (PDH) pathway and aerobic respiration. One unique regulation mode is that a single and the same CRP molecule bound within intergenic regions often regulates both of divergently transcribed operons.
Burden of New and Recurrent Tuberculosis in a Major South African City Stratified by Age and HIV-Status

Aim To describe the burden of tuberculosis (TB) in Cape Town by calculating TB incidence rates stratified by age and HIV-status, assessing the contribution of retreatment disease and estimating the cumulative lifetime TB risk in HIV-negative individuals. Methods Details of TB cases were abstracted from the 2009 electronic TB register. Population denominators were estimated from census data and actuarial estimates of HIV prevalence, allowing calculation of age-specific and HIV-stratified TB notification rates. Results The 2009 mid-year population was 3,443,010 (3,241,508 HIV-negative and 201,502 HIV-positive individuals). There were 29,478 newly notified TB cases of which 56% were laboratory confirmed. HIV status was recorded for 87% of cases and of those with known HIV-status 49% were HIV-negative and 51% were positive. Discrete peaks in the incidence of non-HIV-associated TB occurred at three ages: 511/100,000 at 0–4 years of age, 553/100,000 at 20–24 years and 628/100,000 at 45–49 years with 1.5%, 19% and 45% being due to retreatment TB, respectively. Only 15.5% of recurrent cases had a history of TB treatment failure or default. The cumulative lifetime risks in the HIV-negative population of all new TB episodes and new smear-positive TB episodes were 24% and 12%, respectively; the lifetime risk of retreatment disease was 9%. The HIV-positive notification rate was 6,567/100,000 (HIV-associated TB rate ratio = 17). Although retreatment cases comprised 30% of the HIV-associated TB burden, 88% of these patients had no history of prior treatment failure or default. Conclusions The annual burden of TB in this city is huge. TB in the HIV-negative population contributed almost half of the overall disease burden and cumulative lifetime risks were similar to those reported in the pre-chemotherapy era. Retreatment TB contributed significantly to both HIV-associated and non-HIV-associated TB but infrequently followed prior inadequate treatment. This likely reflects ongoing TB transmission to both HIV-negative and positive individuals.