Miracle-dz/UniFAR-FTUs huggingface dataset:
Extended Reality for Neuraxial Anesthesia and Pain Procedures: A Scoping Review

Background Extended reality technology, encompassing augmented reality, mixed reality, and virtual reality, has the potential to enhance the teaching and performance of neuraxial procedures. The diverse applications of extended reality include immersive simulations and novel modes of procedural navigation. Objectives This scoping review aims to explore the preclinical, clinical, and educational applications of extended reality for neuraxial procedures while suggesting directions for future research. Evidence review A systematic search was conducted across PubMed, Embase, Web of Science, Cochrane Central Register of Controlled Trials, and Google Scholar until December 2023. Additional sources were identified via citation searching of relevant articles. The findings are reported using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). Findings 41 studies, including three pending clinical trials were included. The majority of included studies were published after 2015. Extended reality technology was applied in diverse ways for teaching, simulation, and navigation, but only four of the completed studies described clinical use. For the display of visuals, computer screens were most commonly used, followed by head-mounted devices, laser projectors, and semi-transparent mirrors. Conclusions Interest in utilizing extended reality technology for neuraxial procedures is growing. Preliminary work shows promise for this technology in both education and clinical practice, but achieving accurate image registration without disrupting existing workflows remains an ongoing barrier to clinical testing. Additional research is needed to assess the cost-effectiveness and reliability of this technology.
The Acute Impacts of Resistance Training Performed with and without Blood Flow Restriction on Lower Body Muscular Power

The American College of Sports Medicine recommends resistance training using at least 70% one repetition maximum to improve muscular strength and hypertrophy; however, these intensities may not be safe for all populations. A training technique that has been reported to elicit increases in strength and muscle size uses low intensity resistance training or low load training in combination with blood flow restriction (BFR) to the working muscle. Although the acute effects of BFR on muscle strength and size are well established, the effects of BFR on muscular power are not definitively known. Resistance trained males (n = 14) completed three experimental sessions in which lower body power output and vertical jump height were measured pre and post exercise protocol. The barbell back squat was performed with either low load and blood flow restriction, high load (90% 1 RM, HL), or control (CON). A significant mean difference between pre (M = 46.35 ± 5.61 cm) and post (M = 43.63 ± 4.59 cm) vertical jump heights following 15 repetitions at 20% 1 RM with BFR was observed (p = 0.034), but not with HL or a CON. A decrement in vertical jump height was experienced after an acute bout of BFR with low load resistance exercise. Low load resistance exercise with BFR or high intensity resistance exercise may not be beneficial as part of a warm-up to acutely enhance vertical jump or power output.
The risk of revision is higher following shoulder hemiarthroplasty compared with total shoulder arthroplasty for osteoarthritis: a matched cohort study of 11,556 patients from the National Joint Registry, UK

Background and purpose Total shoulder arthroplasty (TSA) and hemiarthroplasty (HA) are used in the management of osteoarthritis of the glenohumeral joint. We aimed to determine whether TSA or HA resulted in a lower risk of adverse outcomes in patients of all ages with osteoarthritis and an intact rotator cuff and in a subgroup of patients aged 60 years or younger. Patients and methods Shoulder arthroplasties recorded in the National Joint Registry, UK, between April 1, 2012 and June 30, 2021, were linked to Hospital Episode Statistics in England. Elective TSAs and HAs were matched on propensity scores based on 11 variables. The primary outcome was all-cause revision. Secondary outcomes were combined revision/non-revision reoperations, 30-day inpatient complications, 1-year mortality, and length of stay. 95% confidence intervals (CI) were reported. Results 11,556 shoulder arthroplasties were included: 7,641 TSAs, 3,915 HAs. At 8 years 95% (CI 94–96) of TSAs and 91% (CI 90–92) of HAs remained unrevised. The hazard ratio (HR) varied across follow-up: 4-year HR 2.7 (CI 1.9–3.5), 8-year HR 2.0 (CI 0.5–3.5). Rotator cuff insufficiency was the most common revision indication. In patients aged 60 years or younger prosthesis survival at 8 years was 92% (CI 89–94) following TSA and 84% (CI 80–87) following HA. Conclusion The risk of revision was higher following HA in patients with osteoarthritis and an intact rotator cuff. Patients aged 60 years and younger had a higher risk of revision following HA.
Genome‐wide loss‐of‐function genetic screen identifies INSIG2 as the vulnerability of hepatitis B virus‐integrated hepatoma cells

Abstract There are approximately 250 million people chronically infected with hepatitis B virus (HBV) worldwide. Although HBV is often integrated into the host genome and promotes hepatocarcinogenesis, vulnerability of HBV integration in liver cancer cells has not been clarified. The aim of our study is to identify vulnerability factors for HBV‐associated hepatocarcinoma. Loss‐of‐function screening was undertaken in HepG2 and HBV‐integrated HepG2.2. 15 cells expressing SpCas9 using a pooled genome‐wide clustered regularly interspaced short palindromic repeats (CRISPR) library. Genes whose guide RNA (gRNA) abundance significantly decreased in HepG2.2. 15 cells but not in HepG2 cells were extracted using the MAGeCK algorithm. We identified four genes (BCL2L1, VPS37A, INSIG2, and CFLAR) that showed significant reductions of gRNA abundance and thus potentially involved in the vulnerability of HBV‐integrated cancer cells. Among them, siRNA‐mediated mRNA inhibition or CRISPR‐mediated genetic deletion of INSIG2 significantly impaired cell proliferation in HepG2.2. 15 cells but not in HepG2 cells. Its inhibitory effect was alleviated by cotransfection of siRNAs targeting HBV. INSIG2 inhibition suppressed the pathways related to cell cycle and DNA replication, downregulated cyclin‐dependent kinase 2 (CDK2) levels, and delayed the G1‐to‐S transition in HepG2.2. 15 cells. CDK2 inhibitor suppressed cell cycle progression in HepG2.2. 15 cells and INSIG2 inhibition did not suppress cell proliferation in the presence of CDK2 inhibitor. In conclusion, INSIG2 inhibition induced cell cycle arrest in HBV‐integrated hepatoma cells in a CDK2‐dependent manner, and thus INSIG2 might be a vulnerability factor for HBV‐associated liver cancer.
Causal association of attention-deficit/hyperactivity disorder and autism spectrum disorder with post-traumatic stress disorder

Background Attention-deficit/hyperactivity disorder (ADHD) and autism spectrum disorder (ASD) are two neurodevelopmental disorders that often result in individuals experiencing traumatic events. However, little is known about the connection between ADHD/ASD and post-traumatic stress disorder (PTSD). This study aimed to investigate the genetic associations between these disorders. Methods Genetic correlation analysis was used to examine the genetic components shared between ADHD (38 691 cases and 275 986 controls), ASD (18 381 cases and 27 969 controls) and PTSD (23 212 cases and 151 447 controls). Two-sample Mendelian randomization analyses were employed to explore the bidirectional causal relationships between ADHD/ASD and PTSD. Results The results of the genetic correlation analysis revealed significant positive correlations of PTSD with ADHD(rg = 0.70) and ASD (rg = 0.34). Furthermore, the Mendelian randomization analysis revealed that genetic liabilities to ADHD [odds ratio (OR) = 1.14; 95% confidence interval (CI), 1.06–1.24; P = 7.88 × 10−4] and ASD (OR = 1.04; CI, 1.01–1.08; P = 0.014) were associated with an increased risk of developing PTSD later in life. However, no evidence supported that genetic liability to PTSD could elevate the risk of ADHD or ASD. Conclusion The findings of this study supported that ADHD and ASD may increase the risk of PTSD, but not vice versa.
Predicting Anxiety and Depression Among Erectile Dysfunction Patients: A Cross-Sectional Study

In Vietnam, erectile dysfunction (ED) is prevalent and recognized to be associated with mental disorders; however, societal taboos impede a comprehensive understanding of this connection. Our study aims to investigate the factors related to higher levels of anxiety and/or depression (HAD) in individuals with ED. Between November 2022 and March 2023, a face-to-face survey was conducted at the Center for Andrology of Viet Duc University Hospital, involving 390 patients diagnosed with ED. The survey included 51 questions covering general patient information, the International Index of Erectile Function-15 (IIEF-15), the Patient Health Questionnaire-9 (PHQ-9), and the Generalized Anxiety Disorder-7 (GAD-7). The stepwise Akaike Information Criterion (AIC) method was used to identify factors associated with HAD. The study revealed an average age of 37.63 (11.84) years among participants, with a HAD prevalence of 17.69%. Several factors were associated with a higher likelihood of belonging to the HAD group in ED patients. These factors included non-office workers (OR: 1.11; 95% CI: [1.01, 1.21], p = .025), medium and high levels of work-related stress (OR: 1.23; [1.06, 1.44], p = .008; OR: 1.22; [1.04, 1.45], p = .018), multiple shameful experiences related to ED (OR: 1.16; [1.08, 1.25], p < .001), moderate and severe ED (OR: 1.17; [1.03, 1.32], p = .013; OR: 1.31; [1.14, 1.51], p < .001), and dissatisfaction with intercourse skills (OR: 1.09; [1.01, 1.17], p = .028). Our findings suggest a 16% higher likelihood of HAD status in individuals with multiple shameful experiences related to ED, while moderate and severe ED are associated with respective increases of 17% and 31% in the likelihood. These findings emphasize the importance of considering mental health in the care of individuals with ED.
Elucidating fungal decomposition of organic matter at sub-micrometer spatial scales using optical photothermal infrared (O-PTIR) microspectroscopy

ABSTRACT In microbiological studies, a common goal is to link environmental factors to microbial activities. Both environmental factors and microbial activities are typically derived from bulk samples. It is becoming increasingly clear that such bulk environmental parameters poorly represent the microscale environments microorganisms experience. Using infrared (IR) microspectroscopy, the spatial distribution of chemical compound classes can be visualized, making it a useful tool for studying the interactions between microbial cells and their microenvironments. The spatial resolution of conventional IR microspectroscopy has been limited by the diffraction limit of IR light. The recent development of optical photothermal infrared (O-PTIR) microspectroscopy has pushed the spatial resolution of IR microspectroscopy beyond this diffraction limit, allowing the distribution of chemical compound classes to be visualized at sub-micrometer spatial scales. To examine the potential and limitations of O-PTIR microspectroscopy to probe the interactions between fungal cells and their immediate environments, we imaged the decomposition of cellulose films by cells of the ectomycorrhizal fungus Paxillus involutus and compared O-PTIR results using conventional IR microspectroscopy. Whereas the data collected with conventional IR microspectroscopy indicated that P. involutus has only a very limited ability to decompose cellulose films, O-PTIR data suggested that the ability of P. involutus to decompose cellulose was substantial. Moreover, the O-PTIR method enabled the identification of a zone located outside the fungal hyphae where the cellulose was decomposed by oxidation. We conclude that O-PTIR can provide valuable new insights into the abilities and mechanisms by which microorganisms interact with their surrounding environments. IMPORTANCE Infrared (IR) microspectroscopy allows the spatial distribution of chemical compound classes to be visualized. The use of conventional IR microspectroscopy in microbiological studies has been restricted by limited spatial resolution. Recent developments in laser technology have enabled a new class of IR microspectroscopy instruments to be developed, pushing the spatial resolution beyond the diffraction limit of IR light to approximately 500 nm. This improved spatial resolution now allows microscopic observations of changes in chemical compounds to be made, making IR microspectroscopy a useful tool to investigate microscale changes in chemistry that are caused by microbial activity. We show these new possibilities using optical photothermal infrared microspectroscopy to visualize the changes in cellulose substrates caused by oxidation by the ectomycorrhizal fungus Paxillus involutus at the interface between individual fungal hyphae and cellulose substrates.
Identifying cortical structure markers of resilience to adversity in young people using surface-based morphometry

Abstract Previous research on the neurobiological bases of resilience in youth has largely used categorical definitions of resilience and voxel-based morphometry methods that assess gray matter volume. However, it is important to consider brain structure more broadly as different cortical properties have distinct developmental trajectories. To address these limitations, we used surface-based morphometry and data-driven, continuous resilience scores to examine associations between resilience and cortical structure. Structural MRI data from 286 youths (Mage = 13.6 years, 51% female) who took part in the European multi-site FemNAT-CD study were pre-processed and analyzed using surface-based morphometry. Continuous resilience scores were derived for each participant based on adversity exposure and levels of psychopathology using the residual regression method. Vertex-wise analyses assessed for correlations between resilience scores and cortical thickness, surface area, gyrification and volume. Resilience scores were positively associated with right lateral occipital surface area and right superior frontal gyrification and negatively correlated with left inferior temporal surface area. Moreover, sex-by-resilience interactions were observed for gyrification in frontal and temporal regions. Our findings extend previous research by revealing that resilience is related to surface area and gyrification in frontal, occipital and temporal regions that are implicated in emotion regulation and face or object recognition.
The profile of genome-wide DNA methylation, transcriptome, and proteome in streptomycin-resistant Mycobacterium tuberculosis

Streptomycin-resistant (SM-resistant) Mycobacterium tuberculosis (M. tuberculosis) is a major concern in tuberculosis (TB) treatment. However, the mechanisms underlying streptomycin resistance remain unclear. This study primarily aimed to perform preliminary screening of genes associated with streptomycin resistance through conjoint analysis of multiple genomics. Genome-wide methylation, transcriptome, and proteome analyses were used to elucidate the associations between specific genes and streptomycin resistance in M. tuberculosis H37Rv. Methylation analysis revealed that 188 genes were differentially methylated between the SM-resistant and normal groups, with 89 and 99 genes being hypermethylated and hypomethylated, respectively. Furthermore, functional analysis revealed that these 188 differentially methylated genes were enriched in 74 pathways, with most of them being enriched in metabolic pathways. Transcriptome analysis revealed that 516 genes were differentially expressed between the drug-resistant and normal groups, with 263 and 253 genes being significantly upregulated and downregulated, respectively. KEGG analysis indicated that these 516 genes were enriched in 79 pathways, with most of them being enriched in histidine metabolism. The methylation level was negatively related to mRNA abundance. Proteome analysis revealed 56 differentially expressed proteins, including 14 upregulated and 42 downregulated proteins. Moreover, three hub genes (coaE, fadE5, and mprA) were obtained using synthetic analysis. The findings of this study suggest that an integrated DNA methylation, transcriptome, and proteome analysis can provide important resources for epigenetic studies in SM-resistant M. tuberculosis H37Rv.
Glissement moléculaire dans les matériaux lignocellulosiques : mesures de perméabilité apparente et identification de paramètres morphologiques

La permeabilite est l'un de parametres importants pour tous les procedes faisant intervenir des transferts couples de chaleur et de masse. Sa valeur est directement liee a la morphologie du reseau de pores, clairement double echelle dans le cas du bois. Il existe plusieurs outils d’investigation 3-D par exemple la micro-tomographie voire nano-tomographie pour decrire la morphologie des pores. Neanmoins, ces investigations 3-D echouent pour les plus petits pores dans le bois, qui peuvent avoir une taille largement inferieure au micrometre. Ce travail de these utilise l'effet du libre parcours moyen du gaz sur la permeabilite apparente pour identifier la taille des pores utilises par le cheminement du fluide.Une approche equilibree entre experimentation et modelisation est proposee. Dans la premiere partie du travail, nous avons developpe un dispositif original destine a mesurer la permeabilite apparente sur une large plage de niveau de pression moyenne. Ce dispositif ne comporte pas de debitmetre : le flux massique est simplement obtenu par la relaxation de la difference de pression entre deux reservoirs de volume connu.Ce dispositif a permis de mesurer la permeabilite apparente en fonction de la pression moyenne pour differents materiaux. Les valeurs obtenues de permeabilite intrinseque sont en bon accord avec les publications precedentes.L'effet de la pression moyenne sur la permeabilite apparente a ete analyse pour identifier des parametres structuraux des milieux poreux. Partant d'un agencement serie/parallele de pores, les parametres structuraux sont identifies par methode inverse en tirant profit du changement de regime d’ecoulement avec le nombre de Knudsen (du regime de Darcy vers le regime de glissement moleculaire lorsque le nombre de Knudsen passe de zero a l’infini). Cette approche a ete validee avec des membranes a pore monodisperse grâce a une observation en microscopie electronique a balayage. Les parametres ont ensuite ete identifies sur des echantillons de bois, selon differentes directions et sur des materiaux a base de bois. Ils confirment certaines donnees de la litterature, et apportent un eclairage nouveau, notamment sur les chemins de migration transverse chez le hetre.
Neuroimaging of neuropsychological disturbances following ischaemic stroke (CONNECT): a prospective cohort study protocol

Introduction Neuropsychiatric distubance is a common clinical manifestation in acute ischemic stroke. However, it is frequently overlooked by clinicians. This study aimed to explore the possible aetiology and pathogenesis of neuropsychiatric disturbances following ischaemic stroke (NDIS) from an anatomical and functional perspective with the help of neuroimaging methods. Method and analysis CONNECT is a prospective cohort study of neuroimaging and its functional outcome in NDIS. We aim to enrol a minimum of 300 individuals with first-ever stroke. The neuropsychological disturbances involved in this study include depression, anxiety disorder, headache, apathy, insomnia, fatigue and cognitive impairment. Using scales that have been shown to be effective in assessing the above symptoms, the NDIS evaluation battery requires at least 2 hours at baseline. Moreover, all patients will be required to complete 2 years of follow-up, during which the NDIS will be re-evaluated at 3 months, 12 months and 24 months by telephone and 6 months by outpatient interview after the index stroke. The primary outcome of our study is the incidence of NDIS at the 6-month mark. Secondary outcomes are related to the severity of NDIS as well as functional rehabilitation of patients. Functional imaging evaluation will be performed at baseline and 6-month follow-up using specific sequences including resting-state functional MRI, diffusion tensor imaging, T1-weighted imaging, T2-weighted imaging, diffusion-weighted imaging, arterial spin labelling, quantitative susceptibility mapping and fluid-attenuated inversion recovery imaging. In addition, we collect haematological information from patients to explore potential biological and genetic markers of NDIS through histological analysis. Ethics and dissemination The CONNECT Study was approved by the Ethics Review Committee of the First Hospital of the University of Science and Technology of China (2021-ky012) and written informed consent will be obtained from all participants. Results will be disseminated via a peer-reviewed journal. Trial registration number ChiCTR2100043886.
‘Life should go on’: a qualitative inquiry of parental reactions, experiences, and perceived needs following adolescents’ recent traumatic exposure

ABSTRACT Background: Parents have a significant role in supporting children who have been exposed to traumatic events. Little is known about parental experiences and needs in the wake of traumatic exposure, which could help in designing tailored early interventions. Objective: This qualitative study explored experiences, perceived needs, and factors impacting those needs being met, in parents of adolescents aged 11–16 years who had been exposed in the past 3 months to a potentially traumatic event, in the city of Montpellier, France. Method: We purposively sampled 34 parents of 25 adolescents aged 11–16 years meeting the inclusion criteria and used semi-structured in-depth interviews. Thematic analysis was applied using a multistage recursive coding process. Results: Parents lacked trauma-informed explanations to make sense of their child’s reduced functioning. They experienced stigma attached to the victim label and were reluctant to seek help. School avoidance and lack of collaboration with schools were major obstacles experienced by parents. Parents trying to navigate conflicting needs fell into two distinct categories. Those who experienced distressing levels of shame and guilt tended to avoid discussing the traumatic event with their child, pressuring them to resume life as it was before, despite this perpetuating conflictual interactions. Others adapted by revisiting their beliefs that life should go on as it was before and by trying to come up with new functional routines, which improved their relationship with their child and helped them to restore a sense of agency and hope, but at the cost of questioning their parental role. Conclusions: Key domains of parental experiences could provide potential early intervention targets, such as psychoeducation on traumatic stress, representations about recovery and the victim status, parent–child communication, and involvement of schools and primary caregivers. Further research is needed to validate the impact of these domains in early post-traumatic interventions.