Miracle-dz/UniFAR-FTUs huggingface dataset:
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes

This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.
SECOND-GENERATION CEMENTLESS TOTAL HIP ARTHROPLASTY IN PATIENTS WITH OSTEONECROSIS OF THE FEMORAL HEAD

The rate of failure of primary THA in patients with osteonecrosis of the femoral head is higher than that in patients who undergo THA because of other diagnoses. We examined the results of cementless THA performed with second-generation in a consecutive series of young patients with osteonecrosis of the femoral head. Sixty-five consecutive primary THAs with insertion of a femoral stem with a circumferential proximal porous coating (HG Multilock prosthesis) and a cementless acetabular component (Harris-Galante II) were performed in 52 patients with osteonecrosis of the femoral head. These patients were followed prospectively and evaluated at a minimum of 10 years after surgery. Four patients (4 hips) died and three patients (3 hips) were lost to follow-up monitoring. The remaining 45 patients (58 hips) had a mean of 11.1 years (range, 10 to 13.4 years) of clinical and radiographic follow-up. One stem (1.7%) was revised because of aseptic loosening. Eighteen cups (31%) were revised because of excessive polyethylene wear and osteolysis. One hip (1.7%) underwent revision of both acetabular and femoral component because of excessive polyethylene wear and osteolysis. The mean Harrsi Hip Score improved from 49 points before surgery to 92.8 points after surgery in patients who did not undergo reoperation. Osteolysis around the acetabular component was present in 22 hips (37.9%). Femoral osteolysis was seen in 9 hips (15.5%), and there was no osteolysis below the lesser trochanter in any hip. Circumferentially porous-coated second-generation femoral prostheses provide excellent fixation in young patients with osteonecrosis of the femoral head. However, a high rate of polyethylene wear and osteolysis in these high-risk patients remains a challenging problem.
HUMAN RECOMBINANT FSH INDUCES CHEMORESISTANCE IN HUMAN BREAST CANCER CELLS VIA HIF-1α ACTIVATION

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Hiplot: a comprehensive and easy-to-use web service boosting publication-ready biomedical data visualization

Modern web techniques provide an unprecedented opportunity for leveraging complex biomedical data generating in clinical, omics, and mechanism experiments. Currently, the functions for carrying out publication-ready biomedical data visualization represent primary technical hurdles in the state-of-art omics-based web services, whereas the demand for visualization-based interactive data mining is ever-growing. Here, we propose an easy-to-use web service, Hiplot (https://hiplot.com.cn), equipping with comprehensive and interactive biomedical data visualization functions (230+) including basic statistics, multi-omics, regression, clustering, dimensional reduction, meta-analysis, survival analysis, risk modeling, etc. We used the demo and real datasets to demonstrate the usage workflow and the core functions of Hiplot. It permits users to conveniently and interactively complete a few specialized visualization tasks that previously could only be done by senior bioinformatics or biostatistics researchers. A modern web client with efficient user interfaces and interaction methods has been implemented based on the custom components library and the extensible plugin system. The versatile output can also be produced in different environments via using the cross-platform portable command-line interface (CLI) program, Hctl. A switchable view between the editable data table and the file uploader/path selection could facilitate data importing, previewing, and exporting, while the plumber-based response strategy significantly reduced the time costs for generating basic scientific graphics. Diversified layouts, themes/styles, and color palettes in this website allow users to create high-quality and publication-ready graphics. Researchers devoted to both life and data science may benefit from the emerging web service.
Stature, Body Mass, and BMI in High School American Football Players: Appropriate determinants of obesity prevalence?

The purpose of this study was to evaluate stature (HT), weight (WT), body mass index (BMI), and obesity prevalence based on BMI categories in a large sample (n = 7,175) of high school American football players enrolled as freshmen, sophomores, or juniors. Players were categorized by their positions: offensive linemen (OL), defensive linemen (DL), tight end (TE), defensive end (DE), linebacker (LB), running back (RB), quarterback (QB), defensive back (DB), and wide receiver (WR). HT, WT, and BMI increased as grade increased among all positions. OL and DL had the greatest HT, WT, and BMI (p≤0.05). Obesity prevalence was greatest in OL and DL. When accounting for age-related increases in BMI, WT increased to a greater degree than HT. If HT is an indirect indicator of skeletal size, while WT is more influenced by soft tissue, then the age-related BMI increases in the present study may be largely accounted for by soft tissue changes rather than skeletal growth. Even though obesity prevalence in OL (94.5%) and DL (78.4%) positions was greater than all other positions as determined from BMI, it is impossible to know the allocations of fat-free and fat mass—particularly in American football athletes. If obesity continues to be defined as an unhealthy accumulation of fat, then athletes who may have a greater relative proportion of lean soft tissue should not be classified as obese using BMI (WT÷HT2). More sophisticated, reliable, and sensitive measure of body composition, such as skinfolds, may be more appropriate field measurements.
Progress towards elucidating the mechanisms of self-incompatibility in the grasses: further insights from studies in Lolium.

BACKGROUND AND SCOPE Self-incompatibility (SI) in flowering plants ensures the maintenance of genetic diversity by ensuring outbreeding. Different genetic and mechanistic systems of SI among flowering plants suggest either multiple origins of SI or considerable evolutionary diversification. In the grasses, SI is based on two loci, S and Z, which are both polyallelic: an incompatible reaction occurs only if both S and Z alleles are matched in individual pollen with alleles of the pistil on which they alight. Such incompatibility is referred to as gametophytic SI (GSI). The mechanics of grass GSI is poorly understood relative to the well-characterized S-RNase-based single-locus GSI systems (Solanaceae, Rosaceae, Plantaginaceae), or the Papaver recognition system that triggers a calcium-dependent signalling network culminating in programmed cell death. There is every reason to suggest that the grass SI system represents yet another mechanism of SI. S and Z loci have been mapped using isozymes to linkage groups C1 and C2 of the Triticeae consensus maps in Secale, Phalaris and Lolium. Recently, in Lolium perenne, in order to finely map and identify S and Z, more closely spaced markers have been developed based on cDNA and repeat DNA sequences, in part from genomic regions syntenic between the grasses. Several genes tightly linked to the S and Z loci were identified, but so far no convincing candidate has emerged. RESEARCH AND PROGRESS From subtracted Lolium immature stigma cDNA libraries derived from S and Z genotyped individuals enriched for SI potential component genes, kinase enzyme domains, a calmodulin-dependent kinase and a peptide with several calcium (Ca(2+)) binding domains were identified. Preliminary findings suggest that Ca(2+) signalling and phosphorylation may be involved in Lolium GSI. This is supported by the inhibition of Lolium SI by Ca(2+) channel blockers lanthanum (La(3+)) and verapamil, and by findings of increased phosphorylation activity during an SI response.
MicroRNA-24 Is a Novel Regulator of Aldosterone and Cortisol Production in the Human Adrenal Cortex

Dysregulation of aldosterone or cortisol production can predispose to hypertension, as seen in aldosterone-producing adenoma, a form of primary aldosteronism. We investigated the role of microRNA (miRNA) in their production, with particular emphasis on the CYP11B1 (11&bgr;-hydroxylase) and CYP11B2 (aldosterone synthase) genes, which produce the enzymes responsible for the final stages of cortisol and aldosterone biosynthesis, respectively. Knockdown of Dicer1, a key enzyme in miRNA maturation, significantly altered CYP11B1 and CYP11B2 expression in a human adrenocortical cell line. Screening of nondiseased human adrenal and aldosterone-producing adenoma samples yielded reproducible but distinctive miRNA expression signatures for each tissue type, with levels of certain miRNA, including microRNA-24 (miR-24), differing significantly between the 2. Bioinformatic analysis identified putative binding sites for several miRNA, including miR-24, in the 3′ untranslated region of CYP11B1 and CYP11B2 mRNAs. In vitro manipulation of miR-24 confirmed its ability to modulate CYP11B1 and CYP11B2 expression, as well as cortisol and aldosterone production. This study demonstrates that Dicer-dependent miRNA, including miR-24, can post-transcriptionally regulate expression of the CYP11B1 and CYP11B2 genes. Normal adrenal tissue and aldosterone-producing adenoma differ significantly and reproducibly in their miRNA expression profiles, with miR-24 significantly downregulated in the latter. Adrenal miRNA may, therefore, be a novel and valid target for the therapeutic manipulation of corticosteroid biosynthesis.
Evaluation of prenatal care in primary care in the perception of pregnant women

Objective: To evaluate prenatal care in primary health care in the perception of pregnant women. Method: This cross-sectional study analyzes the association and statistical significance of the expectations and satisfaction of pregnant women receiving prenatal care in a municipality of southeast Brazil. We evaluated the association using simple logistic regression and statistical significance between variables and the expectation and satisfaction domains. Results: A predominance of low expectations, with 279 (74%), and high satisfaction, with 220 (58.8%), was identified among the pregnant women regarding their perceptions of prenatal care. Tests showed an association and a statistical significance between the variable “to have used the same prenatal service” with the satisfaction domain. No differences were found between the two service models for both domains. Conclusion: The low expectation and high satisfaction of the pregnant women for the two care models can reveal ways of improving the quality of care in both services.
First-Principles Study of Na Intercalation and Diffusion Mechanisms at 2D MoS2/Graphene Interfaces

Na-ion batteries (NIBs) are emerging as promising energy storage devices for large-scale applications. Great research efforts are devoted to design new effective NIB electrode materials, especially for the anode side. A hybrid 2D heterojunction with graphene and MoS2 has been recently proposed for this purpose: while MoS2 has shown good reversible capacity as a NIB anode, graphene is expected to improve conductivity and resistance to mechanical stress upon cycling. The most relevant processes for the anode are the intercalation and diffusion of the large Na ion, whose complex mechanisms are determined by the structural and electronic features of the MoS2/graphene interface. Understanding these processes and mechanisms is crucial for developing new nanoscale anodes for NIBs with high performances. To this end, here we report a state-of-the-art DFT study to address (a) the structural and electronic properties of heterointerfaces between the MoS2 monolayers and graphene, (b) the most convenient insertion sites for Na, and (c) the possible diffusion paths along the interface and the corresponding energy barrier heights. We considered two MoS2 polymorphs: 1T and 3R. Our results show that 1T-MoS2 interacts more strongly with graphene than 3R-MoS2. In both cases, the best Na host site is found at the MoS2 side of the interface, and the band structure reveals a proper n-type character of the graphene moiety, which is responsible for electronic conduction. Minimum-energy paths for Na diffusion show very low barrier heights for the 3R-MoS2/graphene interface (<0.25 eV) and much higher values for its 1T counterpart (∼0.7 eV). Analysis of structural features along the diffusion transition states allows us to identify the strong coordination of Na with the exposed S atoms as the main feature hindering an effective diffusion in the 1T case. These results provide new hints on the physicochemical details of Na intercalation and diffusion mechanisms at complex 2D heterointerfaces and will help further development of advanced electrode materials for efficient NIBs.
Impaired Lymphocyte Responses in Pediatric Sepsis Vary by Pathogen Type

Background: Sepsis is the leading cause of death in hospitalized children worldwide. Despite its hypothesized immune-mediated mechanism, targeted immunotherapy for sepsis is not available for clinical use. Objective: To determine the association between cytometric, proteomic, bioenergetic, and metabolomic abnormalities and pathogen type in pediatric sepsis. Methods: Serial PBMC samples were obtained from 14 sepsis patients (34 samples) and 7 control patients for this pilot study. Flow cytometry was used to define immunophenotype, including T cell subset frequency and activation state, and assess intracellular cytokine production. Global immune dysfunction was assessed by TNF- production capacity and monocyte HLA-DR expression. Mitochondrial function was assessed by bulk respirometry. Metabolites were measured by liquid chromatography-mass spectrometry. Results were compared by timepoint and pathogen type. For detailed Methods, please see the Methods section in this article's Online Repository at www.jacionline. org. Results: Sepsis patients were older and had higher illness severity compared to controls; demographics were otherwise similar. Compared to controls, sepsis patients demonstrated global immune dysfunction, loss of peripheral of non-naive CD4+ T cells, and reduced PBMC mitochondrial function. Metabolomic findings in sepsis patients were most pronounced at sepsis onset and included elevated uridine and 2-dehydrogluconate and depleted citrulline. Loss of peripheral non-naive CD4+ T cells was associated with immune dysfunction and reduced cytokine production despite increased T cell activation. CD4+ T cell differentiation and corresponding pro- and anti-inflammatory cytokines varied by pathogen. Conclusion: Pediatric sepsis patients exhibit a complex, dynamic physiologic state characterized by immunometabolic dysregulation which varies by pathogen type.
Modeling miRNA-driven post-transcriptional regulation by 1 using exon-intron split analysis ( EISA ) in pigs 2 3

27 Bulk sequencing of RNA transcripts has typically been used to quantify gene expression 28 levels in different experimental systems. However, linking differentially expressed (DE) 29 mRNA transcripts to gene expression regulators, such as miRNAs, remains challenging, 30 as miRNA-mRNA interactions are commonly identified post hoc after selecting sets of 31 genes of interest, thus biasing the interpretation of underlying gene regulatory networks. 32 In this study, we aimed at disentangling miRNA-driven post-transcriptional signals 33 linked to porcine muscle and adipose tissue energy homeostasis. For this purpose, we 34 performed an exon-intron split analysis (EISA) on muscle and fat RNA-seq data from 35 two independent pig populations. One of these populations was subjected to fasting36 feeding conditions, while the other represented divergent fatness profiles. After running 37 EISA, protein-coding mRNA genes with downregulated exonic fractions and high post38 transcriptional signals were significantly enriched for binding sites of DE upregulated 39 miRNAs. Moreover, these downregulated genes showed an increased expression 40 covariation for the exonic fraction compared to the intronic fraction. On the contrary, 41 they did not show enrichment for binding sites of non-DE highly expressed or 42 downregulated DE miRNAs. Among the set of loci displaying miRNA-driven post43 transcriptional regulatory signals, we observed genes related to glucose homeostasis 44 (DKK2, PDK4, IL18, NR4A3, CHRNA1, TET2), cell differentiation (PBX1, BACH2) 45 and adipocytes metabolism (SESN3, ESRRG, SAMD4, LEP, PTGFR, SERPINE2, 46 RNF157, GPLD1, NCF2, OSBPL10, PRSS23). Our results highlighted mRNA genes 47
Modeling miRNA-driven post-transcriptional regulatory 1 signals by using exon-intron split analysis ( EISA ) in pigs 2 3

7 Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, 8 Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. 9 Department of Veterinary and Animal Sciences, Faculty of Health and Medical 10 Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark. 11 Universidad Nacional de Villa María, Villa María, Córdoba, Argentina. 12 Animal Breeding and Genetics Program, Institute for Research and Technology in 13 Food and Agriculture (IRTA), Torre Marimon, 08140 Caldes de Montbui, Spain. 14 Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 15 08193 Bellaterra, Barcelona, Spain. 16
The Use of Density Tracers for the Determination of Dense Medium Cyclone Partition Characteristics

The density tracer technique has for some years been utilised for determination of the separation efficiencies of density separators in the diamond and iron ore industries. At the Julius Kruttschnitt Mineral Research Centre (JKMRC), the technique has been adapted for studies of coal separation, and refined to a point where a Tromp or partition curve for a dense medium cyclone module can be determined quickly and accurately. Test programs using 33 mm tracers in seven Australian coal washeries are reported, and the effects of selected operating variables on separator performances are examined. The density tracer technique is shown to have substantial potential in improvement of day to day washery operation and the detection of unusual operating conditions, and in coal preparation research.
Molecular analysis of the Acinetobacter baumannii biofilm-associated 1 protein 2

26 Acinetobacter baumannii is a multidrug resistant pathogen associated with hospital outbreaks 27 of infection across the globe, particularly in the intensive care unit. The ability of 28 A. baumannii to survive in the hospital environment for long periods is linked to antibiotic 29 resistance and its capacity to form biofilms. Here we studied the prevalence, expression and 30 function of the A. baumannii biofilm-associated protein (Bap) in 24 carbapenem-resistant 31 A. baumannii ST92 strains isolated from a single institution over a 10-year period. The bap 32 gene was highly prevalent, with 22/24 strains positive for bap by PCR. Partial sequencing of 33 bap was performed on the index case strain MS1968 and revealed it to be a large and highly 34 repetitive gene approximately 16 kb in size. Phylogenetic analysis employing a 1,948 amino 35 acid region corresponding to the C-terminus of Bap showed Bap MS1968 clusters with Bap 36 sequences from clonal complex (CC) 2 strains ACICU, TCDC-AB0715 and 1656-2, and is 37 distinct from CC1 strains. Using overlapping PCR, the bap MS1968 gene was cloned and its 38 expression in a recombinant E. coli strain resulted in increased biofilm formation. A Bap- 39 specific antibody was generated and western blot analysis showed the majority of 40 A. baumannii strains expressed an ~200 kDa Bap protein. Further analysis of three Bap- 41 positive A. baumannii strains demonstrated Bap is expressed at the cell surface and is 42 associated with biofilm formation. Finally, biofilm formation by these Bap-positive strains 43 could be inhibited by affinity-purified Bap antibodies, demonstrating the direct contribution 44 of Bap to biofilm growth by A. baumannii clinical isolates.