Miracle-dz/UniFAR-FTUs huggingface dataset:
Innovation network, knowledge absorption ability, and technology innovation performance——An empirical analysis of China’s intelligent manufacturing industry

Based on the social network theory, this study utilizes knowledge absorption capacity as the mediating variable and technology turbulence as the moderating variable; furthermore, it focuses on China’s intelligent manufacturing industry data to explore the effect of the intelligent manufacturing enterprise innovation network on technology innovation performance and the regulating mechanism of technology turbulence. Based on the patent data obtained from Derwent Database (survey period: 2016–2020), the empirical analysis indicates the following: (1) Network relationship, network location, and network density are significantly and positively correlated with technology innovation performance; however, network size exerts no significant effect on technology innovation performance. (2) Network relationship strength, network location, and network density exert significantly positive effects on the two dimensions of knowledge absorption capacity, namely the In-degree and the Out-degree. Network size exerts no significant effect on knowledge absorption capacity. (3) Knowledge absorption capacity exerts a partial mediating effect on the relationship between innovation network and technology innovation performance. (4) The three dimensions of innovation network that exert a significant effect on technology innovation performance are positively correlated with the interaction terms of technology turbulence, which indicates that the interaction terms, namely innovation network and technology turbulence, exert a positive impact on technology innovation performance through knowledge absorption capacity, and that the moderating effect of technology turbulence exerts a role through knowledge absorption capacity. Finally, this study postulates implementations and policy proposals for enhancing the innovation performance of intelligent manufacturing enterprises.
Molecular Sensing and Manipulation of Protein Oligomerization in Membrane Nanotubes with Bolaamphiphilic Foldamers

Adaptive and reversible self-assembly of supramolecular protein structures is a fundamental characteristic of dynamic living matter. However, the quantitative detection and assessment of the emergence of mesoscale protein complexes from small and dynamic oligomeric precursors remains highly challenging. Here, we present a novel approach utilizing a short membrane nanotube (sNT) pulled from a planar membrane reservoir as nanotemplates for molecular reconstruction, manipulation, and sensing of protein oligomerization and self-assembly at the mesoscale. The sNT reports changes in membrane shape and rigidity caused by membrane-bound proteins as variations of the ionic conductivity of the sNT lumen. To confine oligomerization to the sNT, we have designed and synthesized rigid oligoamide foldamer tapes (ROFTs). Charged ROFTs incorporate into the planar and sNT membranes, mediate protein binding to the membranes, and, driven by the luminal electric field, shuttle the bound proteins between the sNT and planar membranes. Using Annexin-V (AnV) as a prototype, we show that the sNT detects AnV oligomers shuttled into the nanotube by ROFTs. Accumulation of AnV on the sNT induces its self-assembly into a curved lattice, restricting the sNT geometry and inhibiting the material uptake from the reservoir during the sNT extension, leading to the sNT fission. By comparing the spontaneous and ROFT-mediated entry of AnV into the sNT, we reveal how intricate membrane curvature sensing by small AnV oligomers controls the lattice self-assembly. These results establish sNT-ROFT as a powerful tool for molecular reconstruction and functional analyses of protein oligomerization and self-assembly, with broad application to various membrane processes.
Transvection between nonallelic genomic positions in Drosophila

Abstract In Drosophila, pairing of maternal and paternal homologous chromosomes can permit trans-interactions between enhancers on one homolog and promoters on another, an example of transvection. Although trans-interactions have been observed at many loci in the Drosophila genome and in other organisms, the parameters that govern enhancer action in trans remain poorly understood. Using a transgenic reporter system, we asked whether enhancers and promoters at nonallelic, but nearby, genomic positions can communication in trans. Using one transgenic insertion carrying the synthetic enhancer GMR and another nearby insertion carrying the hsp70 promoter driving a fluorescent reporter, we show that transgenes separated by 2.6 kb of linear distance can support enhancer action in trans at the 53F8 locus. Furthermore, transvection between the nonallelic insertions can be augmented by a small deletion flanking one insert, likely via changes to the paired configuration of the homologs. Subsequent analyses of other insertions in 53F8 that carry different transgenic sequences demonstrate that the capacity to support transvection between nonallelic sites varies greatly, suggesting that factors beyond the linear distance between insertion sites play an important role. Finally, analysis of transvection between nearby nonallelic sites at other genomic locations shows evidence of position effects, where one locus supported GMR action in trans over a linear distance of over 10 kb, whereas another locus showed no evidence of transvection over a span <200 bp. Overall, our data demonstrate that transvection between nonallelic sites represents a complex interplay between genomic context, interallelic distance, and promoter identity.
ASPSCR-1 and Sirt-5 alleviate Clonorchis liver fluke rCsNOSIP-induced oxidative stress, proliferation, and migration in cholangiocarcinoma cells

Background Clonorchiasis, caused by the infection of Clonorchis sinensis (C. sinensis), is a kind of neglected tropical disease, but it is highly related to cholangiocarcinoma. It has been well known that NO from chronic inflammation responses are thought to be a major component of the damage and ultimate carcinogenesis ESPs such as nitric oxide synthase interacting protein (NOSIP) are thought to enhance the damage. The objective of this study was to identify the protein candidates interact with recombinant CsNOSIP (rCsNOSIP) and explore their role involved in CCA development or progression. Methods We applied HuProt microarray containing 21,000 probe sets for a systematic identification of rCsNOSIP-binding proteins and grouped binding hits by gene function. Pull-down assays were used to confirm the interaction of rCsNOSIP with alveolar soft part sarcoma (ASPSCR-1) and sirtuins 5 (Sirt-5). ASPSCR-1/Sirt-5 over-expression and siRNA knockdown experiments were employed for obtain of ASPSCR-1/Sirt-5 high or low expression (ASP-oe/Sirt5-oe or ASP-si/Sirt5-si) cholangiocarcinoma cell line (CCLP-1) cells. Nitric oxide (NO) and reactive oxygen species assay (ROS) as well as cell proliferation and wound-healing assays were performed to observe the effect of rCsNOSIP on ASP-oe/Sirt5-oe or ASP-si/Sirt5-si CCLP-1 cells. Results Seventy candidate proteins protein "hits" were detected as rCsNOSIP-binding proteins by HuProt microarray and bioinformatics analysis. Pull down assay showed that ASPSCR-1 and Sirt-5 could interact with rCsNOSIP. In addition, endotoxin-free-rCsNOSIP could increase the production of NO and ROS and promote the migration of CCLP-1 cells, while its effect on enhancing cell proliferation was not significant. Furthermore, ROS/NO production, proliferation, or migration were increased in ASP-si or Sirt5-si CCLP-1 cells but decreased in Asp-oe or Sirt5-oe CCLP-1 cells when stimulated with rCsNOSIP. Conclusions Our findings suggest that CsNOSIP as a component of CsESPs might promote the development and invasion of CCA and Sirt5/ ASPSCR1 as host molecules might play a novel protective role against adverse stimulus during C. sinensis infection. This work supports the idea that CsESPs induce the occurrence and progression of CCA through ROS/RNS-induced oxidative and nitrative DNA damage.
The Chemistry Behind the Folin–Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern

The Folin–Ciocalteu assay is a reference method for the quantification of total (poly)phenols in food. This review explains the fundamental mechanism of the redox reaction on which the method is based and looks at some of the practical considerations concerning its application. To accurately estimate the antioxidant capacity of (poly)phenolic compounds, a thorough knowledge of their structural characteristics is essential, as the two are closely associated. Therefore, to help researchers interpret the results of the Folin–Ciocalteu method, this review also summarizes some of the main phenolic structural features. Finally, we have used the Folin–Ciocalteu method to estimate the total phenolic intake associated with high adherence to a Mediterranean diet, ranked as one of the healthiest dietary patterns, which is characterized by a high consumption of (poly)phenol-rich food such as wine, virgin olive oil, fruits, vegetables, whole grains, nuts, and legumes.
Ultrahigh responsivity of non-van der Waals Bi2O2Se photodetector

Bismuth oxyselenide has recently gained tremendous attention as a promising 2D material for next-generation electronic and optoelectronic devices due to its ultrahigh mobility, moderate bandgap, exceptional environmental stability, and presence of high-dielectric constant native oxide. In this study, we have synthesized single-crystalline nanosheets of Bismuth oxyselenide with thicknesses measuring below ten nanometers on Fluorophlogopite mica using an atmospheric pressure chemical vapor deposition system. We transferred as-grown samples to different substrates using a non-corrosive nail polish-assisted dry transfer method. Back-gated Bi2O2Se field effect transistors showed decent field effect mobility of 100 cm2 V−1s−1. The optoelectronic property study revealed an ultrahigh responsivity of 1.16 × 106 A W−1 and a specific detectivity of 2.55 × 1013 Jones. The samples also exhibited broadband photoresponse and gate-tunable photoresponse time. These results suggest that Bi2O2Se is an excellent candidate for future high-performance optoelectronic device applications.
Emulation of Pavlovian conditioning and pattern recognition through fully connected neural networks using Holmium oxide (Ho2O3) based synaptic RRAM device

In this manuscript, we report on the paramagnetic Ho2O3-based synaptic resistive random-access memory device for the implementation of neuronal functionalities such as long-term potentiation, long-term depression and spike timing dependent plasticity respectively. The plasticity of the artificial synapse is also studied by varying pulse amplitude, pulse width, and pulse interval. In addition, we could classify handwritten Modified National Institute of Standards and Technology data set (MNIST) using a fully connected neural network (FCN). The device-based FCN records a high classification accuracy of 93.47% which is comparable to the software-based test accuracy of 97.97%. This indicates the highly optimized behavior of our synaptic device for hardware neuromorphic applications. Successful emulation of Pavlovian classical conditioning for associative learning of the biological brain is achieved. We believe that the present device consists the potential to utilize in neuromorphic applications.
Ultrasound-mediated delivery of Pik3cb shRNA using magnetic nanoparticles for the treatment of in-stent restenosis in a rat balloon-injured model

Abstract The aim of the present work was to examine the effect of polyethylene glycol (PEG)-coated superparamagnetic iron oxide (SPIO) nanoparticles carrying Pik3cb short hairpin RNA (shRNA) in the prevention of restenosis with the aid of ultrasound and a magnetic field. SPIO is a type of contrast agent used in medical imaging to enhance the visibility of specific tissues or organs. It consists of tiny iron oxide nanoparticles that can be targeted to specific areas of interest in the body. PEG-coated SPIO nanoparticles carrying Pik3cb shRNA (SPIO-shPik3cb) were prepared, and the particle size and zeta potential of PEG-coated SPIO nanoparticles with and without Pik3cb shRNA were examined. After a right common artery balloon-injured rat model was established, the rats were randomly divided into four groups, and the injured arteries were transfected with SPIO-shPik3cb, saline, SPIO-shcontrol and naked shRNA Pik3cb. During the treatment, each group was placed under a magnetic field and was transfected with the aid of ultrasound. Rats were sacrificed, and the tissue was harvested for analysis after 14 days. The results suggested that the mean particle size and zeta potential of SPIO-shPik3cbs were 151.45 ± 11 nm and 10 mV, respectively. SPIO-shPik3cb showed higher transfection efficiency and significantly inhibited the intimal thickening compared with naked Pik3cb shRNA in vascular smooth muscle cells (VSMCs) (*P < 0.05). Moreover, SPIO-shPik3cb could also significantly downregulate the expression of pAkt protein compared with naked Pik3cb shRNA. According to the results, SPIO-shPik3cb can remarkably inhibit the intimal thickening under a combination of magnetic field exposure and ultrasound.
CD4+ T cells reverse surface antigen persistence in a mouse model of HBV replication

ABSTRACT Hepatitis B virus (HBV) is responsible for over 800,000 deaths yearly worldwide. While chronic HBV infection currently has no highly effective cure, immunotherapy has generated interest as a potential therapeutic solution due to the connection between viral chronicity and the magnitude of HBV-specific immune responses. Suitable animal models for studying successful immune responses to HBV are limited due to the lack of natural viral tropism for rodents. Using a mouse model of HBV replication, we found that CD4+ T cells are essential for the antibody-mediated clearance of hepatitis B surface antigen (HBsAg) and that early transient inhibition of the CD4+ T cell-B cell axis imparts HBsAg persistence through CD4+ T cell functional impairment. HBsAg resolution in BALB/c mice transduced with an adeno-associated virus encoding the HBV genome (AAV-HBV) depended on B cells, MHCII, and CD4+ T cells, but not CD8+ T cells, indicative of antibody-mediated control. Furthermore, disrupting the CD40-CD40L interaction blunted HBsAg clearance and the generation of HBsAg-specific B cells. Finally, HBsAg persistence induced by transient CD4 depletion was reversible by the transfer of total splenocytes or purified CD4+ T cells from immunized mice. These findings demonstrate the T cell-dependent nature of HBsAg-resolving antibody responses as well as the ability of CD4+ T cells to reverse HBsAg persistence. IMPORTANCE Hepatitis B virus (HBV) is a leading causative agent of viral hepatitis. A preventative vaccine has existed for decades, but only limited treatment options are available for people living with chronic HBV. Animal models for studying HBV are constrained due to narrow viral tropism, impeding understanding of the natural immune response to the virus. Here, using a vector to overcome the narrow host range and establish HBV replication in mice, we identified the role of helper T cells in controlling HBV. We show that helper T cells promote the B cell’s ability to generate antibodies that remove HBV and its associated surface antigen from the blood and that transfer of purified helper T cells from HBV-immunized mice can reverse the accumulation of virus and antigen, furthering our understanding of the immune response to HBV. Hepatitis B virus (HBV) is a leading causative agent of viral hepatitis. A preventative vaccine has existed for decades, but only limited treatment options are available for people living with chronic HBV. Animal models for studying HBV are constrained due to narrow viral tropism, impeding understanding of the natural immune response to the virus. Here, using a vector to overcome the narrow host range and establish HBV replication in mice, we identified the role of helper T cells in controlling HBV. We show that helper T cells promote the B cell’s ability to generate antibodies that remove HBV and its associated surface antigen from the blood and that transfer of purified helper T cells from HBV-immunized mice can reverse the accumulation of virus and antigen, furthering our understanding of the immune response to HBV.
Water quality in cutaway peatland lakes in Seda mire, Latvia

Water quality in cutaway peatland lakes in Seda mire, Latvia Transformation into shallow lakes can be a major post-harvesting land-use option for cutaway peatlands. The aim of our study was to analyse factors influencing water quality and communities on lakes created in a cutaway bog in the Seda Mire, Latvia. The residual peat amount and the lake feeding conditions (the balance between ground water and inflow from adjacent river during the spring season) were found to be major factors influencing aquatic chemistry and studied habitats. The studied cutaway lakes cannot be considered as typical bog lakes regarding hydrochemical composition (especially regarding concentrations of major dissolved ions, pH and water colour) and hydrobiological features (metabolic activity, biomass and number of species of phytoplankton and zoobenthos). Considering aquatic chemistry and community structure of the cutaway lakes, a recommendation was made in respect to peat excavation to transform peat mining sites to lakes. Ūdeņu kvalitāte kūdras karjeru ezeros Sedas purvā Pēdējos gados kā viena no iespējām izstrādāto kūdras karjeru rekultivācijai tiek piedāvāta seklu ezeru veidošana. Šī darba ietvaros tika pētīti applūdušie kūdras karjeri Sedas purvā ar mērķi novērtēt ūdens kvalitāti un noteikt galvenos faktorus, kas ietekmē ūdens kvalitāti applūdušajās teritorijās. Pētījumā konstatēts, ka Sedas purva teritorijās esošo applūdušo kūdras karjeru ķīmiskais sastāvs (īpaši pēc galveno neorganisko jonu satura, pH un ūdens krāsainības), kā arī makrozoobentosa, fitoplanktona un mikroorganismu cenozes atšķiras no tipisku purva ezeru ūdeņu kvalitātes rādītājiem. Tas varētu liecināt par to, ka ūdeņu sastāvu apsekotajās ūdenstilpēs ietekmē teritorijas geologiskā uzbūve un gruntsūdeņu ķīmiskais sastāvs, jo pēc kūdras izsmelšanas karjeros nav atstāts pietiekams kūdras slānis. Turklāt Sedas purva applūdušajos karjeros pavasara palu laikā vērojama arī virszemes ūdeņu pieplūde no Sedas upes. Jau tuvākajā nākotnē būtu nepieciešams noteikt izstrādātā mitrāja atjaunošanas mērķi konkrētās teritorijās — dabiska regenerācija ar minimālu cilvēka iejaukšanos vai arī mērķtiecīgi virzīta darbība, lai veidotu biologiski daudzveidīgas un saimnieciski izmantojamas platības.
Similar photosynthetic but different yield responses of C3 and C4 crops to elevated O3

Significance Current ground-level ozone (O3) pollution significantly reduces global crop productivity. Understanding how different crops respond to elevated O3 concentration ([O3]) is critical to improve crop production and resilience under atmospheric change. Here, we synthesize available literature and unpublished data from five C3 crops and four C4 crops grown with increased O3 pollution in open-air field experiments over the past 20 y. We quantitatively show that C3 crops are more sensitive to elevated [O3] than C4 crops. Our results provide key insights into O3 response in crops with different photosynthetic pathways and could help guide future efforts to improve crop O3 tolerance.
Estimación de las categorías marxianas mediante tablas de insumo-producto. Un análisis comparativo de Argentina y Estados Unidos

The concrete way in which the process of the valorization of capital occurs in the periphery countries differs substantially from what happens in the core nations. Empirical studies aimed at taking account of these particularities should, in our view, comprise the cycle of capital in each country as a whole. In the present work, we make an estimate of the Marxist categories based on input-output tables for the United States and Argentina for the period 1997-2004. This estimate enables us to carry out a detailed comparison of all of the moments in the capital cycle of a core and a periphery economy, providing a methodologically consistent framework for empirical application of the analytical categories. The results obtained from comparing each category allow us to conclude that structural differences exist between the United States and Argentina at each moment of the cycle of the valorization of capital.
Clinical Determinants of Longitudinal Disability in LGI-1-IgG Autoimmune Encephalitis

Background and Objectives Longitudinal outcome studies in leucine-rich glioma inactivated-1 (LGI-1) immunoglobulin G (IgG) autoimmune encephalitis (AE) are needed to inform clinical management and prognostication. This study aims to evaluate longitudinal predictors of disability and disease severity in LGI-1-IgG AE. Methods This retrospective observational study of patients with LGI-1-IgG AE was conducted between 2013-2022. Disability and disease severity were defined by scores on the modified Rankin Scale (mRS) and the clinical assessment scale in AE (CASE), respectively. Demographic variables, clinical/paraclinical data, brain MRI, and Montreal Cognitive Assessment (MOCA) scores were examined as predictors of mRS and CASE scores in logistic and linear regression models, respectively. Results Thirty patients (60% male, median age = 68.5; interquartile range (IQR) = 63.0–75.0) were included, with a median follow-up time of 19.1 months (IQR = 5.3–47.1) The majority developed seizures (29, [97%]) and/or cognitive impairment (30, [100%]) and received acute (27, [90%]) and maintenance (23 [77%]) immunotherapy. The median initial MOCA was 23/30 (IQR = 21.0–25.0). Baseline mRS (median = 2.0, IQR = 2.0–3.0) and CASE (mean = 4.3, SD = 3.7) correlated with one another (r = 0.58, p < 0.001) and with initial MOCA score (mRS r = –0.60, p = 0.012; CASE r = –0.56, p = 0.021) After 12 months from symptom onset, mRS (OR = 0.88, [95% CI = 0.82–0.94], p < 0.001) and CASE (β = −0.03, [SE = 0.01], p < 0.001) improved significantly. Lower initial MOCA score (OR = 0.68, 95% CI = 0.47–0.98, p = 0.041) and temporal lobe(s) T2 hyperintensity (OR = 16.50, 95% CI = 2.29–119.16, p = 0.006) were associated with higher mRS longitudinally. At last follow-up, most patients had persistent memory dysfunction (25, [83%]) while few had ongoing seizure activity (3, [10%]). Discussion Overall, there was a high degree of correlation between mRS and CASE scores in patients with LGI-1-IgG AE, with both scores improving significantly after 12 months. Memory dysfunction and psychiatric disturbance were the most prevalent longitudinal symptoms. Cognitive impairment and temporal lobe T2 hyperintensity at baseline were both associated with greater disability at long-term follow-up, underscoring these as important determinants of disability outcomes in LGI-1-IgG AE.