Miracle-dz/UniFAR-FTUs huggingface dataset:
Experimental Models of Allergic Disease Cdt Cells Regulate Chronic Airway Inflammation and Development of Airway Remodelling

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Summary Background cdT cells play a crucial immunoregulatory role in the lung, maintaining normal airway tone and preventing hyperresponsiveness to innocuous allergen. During acute inflammatory episodes, cdT cells promote resolution of acute inflammation. However, their contribution to inflammation-associated airway remodelling remains unexplored. Here we investigate the effects of cdT cell blockade on established allergic airway inflammation and development of remodelling. Methods Sensitised mice were exposed to prolonged ovalbumin challenge or continuous house-dust mite exposure to induce chronic inflammation and remodelling. Functional blocking anti-TCRd antibody was administered therapeutically, and parameters of airway inflammation and remodelling were examined. Results Therapeutic blockade of cdT cells prevented the typical resolution of acute airway inflammation characterised by elevated eosinophil and Th2 cell numbers. Moreover, the lung displayed exacerbated airway remodelling, typified by excess peribronchiolar colla-gen deposition. Conclusions These results demonstrate a unique role for cdT cells in constraining aller-gen-induced airway remodelling. Manipulating the cdT cell compartment may therefore contribute to strategies to prevent and treat remodelling.
Geomorphic effects of wood in rivers

—Wood has been falling into rivers for millions of years, resulting in both local effects on channel processes and integrated influences on channel form and dynamics over a wide range of spatial and temporal scales. Effects of stable pieces of wood on local channel hydraulics and sediment transport can influence rates of bank erosion, create pools, or initiate sediment deposition and bar formation. At larger spatial scales, changes in the supply of large wood can trigger changes in both river-reach morphology and the interaction between a river and its floodplain. Over long time scales, wood-rich rivers may retain more sediment and have lower sediment transport rates and steeper slopes than comparable wood-poor channels. Most geomorphic effects of wood in rivers arise from large, stable logs that catalyze changes in the routing and storage of both smaller wood and sediment. The size of a log relative to the channel provides a reasonable gauge of the potential stability of in-channel wood. Channels with a high supply of large, potentially stable wood may experience substantial vertical variability in bed elevation independent from external forcing (e.g., climate variability, temporal variations in sediment supply, or tectonic activity). In some river systems, changes in the wood regime, as described by the size and amount of wood supplied to a river, can result in effects as great as those arising from changes in the sediment supply or the discharge regimes. Consequently, an understanding of the geomorphic effects of wood is crucial for assessing the condition and potential response of forest channels.
Exhaled particles as markers of small airway inflammation in subjects with asthma

Exhaled breath contains suspended particles of respiratory tract lining fluid from the small airways. The particles are formed when closed airways open during inhalation. We have developed a method called Particles in Exhaled air (PExA®) to measure and sample these particles in the exhaled aerosol. Here, we use the PExA® method to study the effects of birch pollen exposure on the small airways of individuals with asthma and birch pollen allergy. We hypothesized that birch pollen‐induced inflammation could change the concentrations of surfactant protein A and albumin in the respiratory tract lining fluid of the small airways and influence the amount of exhaled particles. The amount of exhaled particles was reduced after birch pollen exposure in subjects with asthma and birch pollen allergy, but no significant effect on the concentrations of surfactant protein A and albumin in exhaled particles was found. The reduction in the number of exhaled particles may be due to inflammation in the small airways, which would reduce their diameter and potentially reduce the number of small airways that open and close during inhalation and exhalation.
AMPA-induced excitotoxic lesions of the basal forebrain: a significant role for the cortical cholinergic system in attentional function

The aim of the present study was to clarify the role of the basal forebrain (BF)-cortical cholinergic system in visual attentional function by investigating the effect of alpha-amino-3-hydroxy-5-methyl- 4-isoxazole propionic acid (AMPA)-induced lesions of the basal forebrain on performance of a five-choice serial reaction time task. AMPA lesions in the present study produced a profound effect on performance of the task, as measured by choice accuracy and correct response latency. This deficit was significantly greater than that observed in earlier studies following ibotenate- or quisqualate-induced lesions of the BF. However, detailed histological and biochemical analysis revealed three rather different BF lesions depending upon the batch of AMPA supplied. In one group of animals (BF/1) the deficits in task performance were substantially greater and longer lasting compared to another group of lesioned animals (BF/2), which showed behavioral recovery several months following the lesion. The former sustained severe pallidal damage in addition to marked reductions in cortical ChAT activity. Support for the attentional nature of these deficits was obtained by the ability to improve task performance in BF/1 lesioned animals by increasing the duration of the visual stimulus and thus reducing the attentional load placed on these animals. In contrast, performance deficits could be reinstated in those animals showing behavioral recovery (BF/2) by reducing the duration of the visual stimulus and thus increasing attentional load. In the second experiment more discrete lesions of the magnocellular cholinergic neurons were made, resulting in extensive reduction of cortical ChAT activity with considerably less neuronal loss from the dorsal pallidum compared to the BF/1 lesion group. Once again, deficits on the task were substantially greater than observed previously following either quisqualate- or ibotenate-induced BF lesions. Furthermore, the cholinergic specificity of these deficits was supported by the attenuation of behavioral impairments following administration of the anti-cholinesterase physostigmine. Taken together with our earlier work, which has failed to demonstrate mnemonic deficits following lesions to the magnocellular neurons of the nucleus basalis of Meynert, these results suggest that the most consistent deficit produced following lesions of the BF-cortical cholinergic system is attentional dysfunction Analogous deficits in visual attention are also seen in patients with Alzheimer's disease, which can also be improved by anti- cholinesterase treatment.
Mortality and morbidity in gastro-oesophageal cancer surgery: initial results of ASCOT multicentre prospective cohort study

Abstract Objective To evaluate the effect of comorbidity and other risk factors on postoperative mortality and morbidity in patients undergoing major oesophageal and gastric surgery. Design Multicentre cohort study with data on postoperative mortality and morbidity in hospital. Data source and methods The ASCOT prospective database, comprising 2087 patients with newly diagnosed oesophageal and gastric cancer in 24 hospitals in England and Wales between 1 January 1999 and 31 December 2002. Multivariate logistic regression analysis was used to model the risk of death and postoperative complications. Results 955 patients underwent oesophagectomy or gastrectomy. Of these, 253 (27%) were graded ASA III or IV, and 187 (20%) had a high physiological POSSUM score (≥ 20). Operative mortality was 12% (111/955). Physiological POSSUM score, surgeon's assessment, type of operation, hospital case volume, and tumour stage independently predicted operative mortality. Medical complications were associated with higher physiological POSSUM scores and ASA grade, oesophagectomy or total gastrectomy, thoracotomy, and radical nodal dissection. Stage and additional organ resection predicted surgical (technical) complications. Conclusions Many patients undergoing surgery for gastro-oesophageal cancer have major comorbid disease, which strongly influences their risk of postoperative death. Technical complications do not seem to be influenced by preoperative factors but reflect the extent of surgery and perhaps surgical judgment. Detailed prospective multicentre cooperative audit, with appropriate risk adjustment, is fundamental in the evaluation of cancer care and must be properly resourced.
Prediction Intervals to Account for Uncertainties in Travel Time Prediction

The accurate prediction of travel times is desirable but frequently prone to error. This is mainly attributable to both the underlying traffic processes and the data that are used to infer travel time. A more meaningful and pragmatic approach is to view travel time prediction as a probabilistic inference and to construct prediction intervals (PIs), which cover the range of probable travel times travelers may encounter. This paper introduces the delta and Bayesian techniques for the construction of PIs. Quantitative measures are developed and applied for a comprehensive assessment of the constructed PIs. These measures simultaneously address two important aspects of PIs: 1) coverage probability and 2) length. The Bayesian and delta methods are used to construct PIs for the neural network (NN) point forecasts of bus and freeway travel time data sets. The obtained results indicate that the delta technique outperforms the Bayesian technique in terms of narrowness of PIs with satisfactory coverage probability. In contrast, PIs constructed using the Bayesian technique are more robust against the NN structure and exhibit excellent coverage probability.
Protein B5 is required on extracellular enveloped vaccinia virus for repulsion of superinfecting virions

Vaccinia virus (VACV) spreads across cell monolayers fourfold faster than predicted from its replication kinetics. Early after infection, infected cells repulse some superinfecting extracellular enveloped virus (EEV) particles by the formation of actin tails from the cell surface, thereby causing accelerated spread to uninfected cells. This strategy requires the expression of two viral proteins, A33 and A36, on the surface of infected cells and upon contact with EEV this complex induces actin polymerization. Here we have studied this phenomenon further and investigated whether A33 and A36 expression in cell lines causes an increase in VACV plaque size, whether these proteins are able to block superinfection by EEV, and which protein(s) on the EEV surface are required to initiate the formation of actin tails from infected cells. Data presented show that VACV plaque size was not increased by expression of A33 and A36, and these proteins did not block entry of the majority of EEV binding to these cells. In contrast, expression of proteins A56 and K2 inhibited entry of both EEV and intracellular mature virus. Lastly, VACV protein B5 was required on EEV to induce the formation of actin tails at the surface of cells expressing A33 and A36, and B5 short consensus repeat 4 is critical for this induction.
Interocean Exchange of Thermocline Water

Formation of North Atlantic Deep Water (NADW) represents a transfer of upper layer water to abyssal depths at a rate of 15 to 20 x 10 6 m3/s. NADW spreads throughout the Atlantic Ocean and is exported to the Indian and Pacific Oceans by the Antarctic Circumpolar Current and deep western boundary currents. Naturally, there must be a compensating flow of upper layer water toward the northern North Atlantic to feed NADW production. It is proposed that this return flow is accomplished primarily within the ocean's warm water thermocline layer. In this way the main thermoclines of the ocean are linked as they participate in a thermohaline-driven global scale circulation cell associated with NADW formation. The path of the return flow of warm water is as follows: Pacific to Indian flow within the Indonesian Seas, advection across the Indian Ocean in the 10o-15oS latitude belt, southward transfer in the Mozambique Channel, entry into the South Atlantic by a branch of the Agulhas Current that does not complete the retroflection pattern, northward advection within the subtropical gyre of the South Atlantic (which on balance with the southward flux of colder North Atlantic Deep Water supports the northward oceanic heat flux characteristic of the South Atlantic), and cross-equatorial flow into the western North Atlantic. The magnitude of the return flow increases along its path as more NADW is incorporated into the upper layer of the ocean. Additionally, the water mass characteristics of the return flow are gradually altered by regional ocean-atmosphere interaction and mixing processes. Within the Indonesian seas there is evidence of strong vertical mixing across the thermocline. The cold water route, Pacific to Atlantic transport of Subantarctic water within the Drake Passage, is of secondary importance, amounting to perhaps 25% of the warm water route transport. The continuity or vigor of the warm water route is vulnerable to change not only as the thermohaline forcing in the northern North Atlantic varies but also as the larger-scale wind-driven criculation factors vary. The interocean links within the Indonesian seas and at the Agulhas retroflection may be particularly responsive to such variability. Changes in the warn: water route continuity may in turn influence formation characteristics of NADW.
Molecular Characterization and Functional Analysis of Murine Interleukin 4 Receptor Allotypes

The murine interleukin 4 receptor (IL-4R) exists as a transmembrane protein transducing pleiotropic IL-4 functions, or as soluble (s)IL-4–binding molecule with potent immunoregulatory effects. In this study we identified and characterized a murine IL-4R allotype. Sequence analysis of the IL-4R cDNA of BALB/c mice revealed 18 base substitutions leading to three extracellular and five cytoplasmic amino acid changes when compared with the published IL-4R sequence of C57BL/6 mice. Analyses with allotype-specific mAbs revealed that AKR/J and SJL/J mice possess the newly identified BALB/c IL-4R allotype whereas the IL-4Rs of C3H, CBA, DBA-2, and FVB/N mice are identical to that of the C57BL/6 mouse. The extracellular Thr49 to Ile substitution abrogates one N-glycosylation site in the naturally occurring BALB/c IL-4R as well as in the experimentally point mutated C57BL/6-T49I sIL-4R, and both molecules display a nearly threefold reduction in IL-4–neutralizing activity compared to the C57BL/6 sIL-4R. In line with this, a significantly enhanced dissociation rate of IL-4 was detected for the BALB/c IL-4R allotype by surface plasmon resonance and in radioligand binding studies with IL-4R–transfected cell lines. These findings suggest that the altered ligand binding behavior of the newly described IL-4R allotype may influence the IL-4 responsiveness, thus contributing to the diverse phenotypes of inbred mouse strains in IL-4–dependent diseases.
Clinical Profile of Obstetric Patients Admitted to the Medical-Surgical Intensive Care Unit (MSICU) of an Inner-City Hospital in New York

Background Pregnancy is associated with physiological and anatomical changes that usually occur uneventfully in majority of women. However, these changes can cause major maternal morbidity with potential catastrophic consequences. The purpose of this study is to evaluate the clinical characteristics of obstetric patients admitted to the MSICU of an inner-city hospital in New York. Methods A prospective, observational study was conducted among all the obstetric patients admitted to the MSICU between June 1, 2009 and June 30, 2012. Results A total of 19 obstetric patients were admitted to the MSICU between June 1, 2009 and June 30, 2012. The most common comorbidity on admission was hypertensive disorder. Hemodynamic unstability and shock was the most common admission diagnosis. The mean length of stay was 3.5 days. One patient died. Conclusions Obstetric hemorrhage and pregnancy-induced hypertensive disorders remains as the most common entities requiring intensive care unit (ICU) admission among obstetric patients. A multidisciplinary team involvement is essential in the management of these patients.
Heat-Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons

ABSTRACT Cholera toxin (CT) and the related heat-labile enterotoxins (LT) of Escherichia coli have been implicated as adjuvants in human therapies, but reactivity upon intranasal delivery dampened efforts to develop other clinical applications. However, each CT family member variant has unique biological properties that may warrant development as therapeutic platforms. In the current study, a nontoxic variant of the heat-labile enterotoxin IIa (LTIIa) was engineered to deliver heterologous, functional proteins into the cytosol of neurons. As proof of principle, the LTIIa variant delivered two cargos into neurons. LTIIa delivered β-lactamase efficiently into cells containing complex gangliosides, such as GD1b, as host receptors. LTIIa delivery of β-lactamase was sensitive to brefeldin A, an inhibitor that collapses the Golgi compartment into the endoplasmic reticulum, but not sensitive to treatment with botulinum neurotoxin D (BoNT/D), an inhibitor of synaptic vesicle cycling. LTIIa delivered a single-chain, anti-BoNT/A camelid antibody that inhibited SNAP25 cleavage during post-BoNT/A exposure of neurons. Delivery of functional, heterologous protein cargos into neurons demonstrates the potential of LTII variants as platforms to deliver therapies to inactivate toxins and microbial infections and to reverse the pathology of human neurodegenerative diseases. IMPORTANCE This study engineered a protein platform to deliver functional, heterologous proteins into neurons. The protein platform developed was a variant of the heat-labile enterotoxin IIa (LTIIa) which lacked the catalytic domain, yielding a nontoxic protein. As proof of principle, LTIIa variants delivered two functional proteins into neurons, β-lactamase and a camelid antibody. These studies show the utility of LTIIa variants to deliver therapies into neurons, which could be extended to inactivate toxins and microbial infections and potentially to reverse the progression of neurological diseases, such as Alzheimer's disease and Parkinson's disease. This study engineered a protein platform to deliver functional, heterologous proteins into neurons. The protein platform developed was a variant of the heat-labile enterotoxin IIa (LTIIa) which lacked the catalytic domain, yielding a nontoxic protein. As proof of principle, LTIIa variants delivered two functional proteins into neurons, β-lactamase and a camelid antibody. These studies show the utility of LTIIa variants to deliver therapies into neurons, which could be extended to inactivate toxins and microbial infections and potentially to reverse the progression of neurological diseases, such as Alzheimer's disease and Parkinson's disease.
Particlelike solutions to the Yang-Mills-dilaton system in d = 4 + 1 dimensions

We construct static solutions to a SU(2) Yang–Mills (YM) dilaton model in 4+1 dimensions subject to bi-azimuthal symmetry. The YM sector of the model consists of the usual YM term and the next higher order term of the YM hierarchy, which is required by the scaling condition for the existence of finite energy solutions. The basic features of two different types of configurations are studied, corresponding to (multi)solitons with topological charge n 2 , and soliton–antisoliton pairs with zero topological charge.
Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth

Stem cells from human exfoliated deciduous teeth (SHEDs) have great potential to treat various dental-related diseases in regenerative medicine. They are usually maintained with 10% fetal bovine serum (FBS) in vitro. Modified platelet-rich plasma (mPRP) would be a safe alternative to 10% FBS during SHEDs culture. Therefore, our study aimed to compare the proliferation and differentiation of SHEDs cultured in mPRP and FBS medium to explore an optimal concentration of mPRP for SHEDs maintenance. Platelets were harvested by automatic blood cell analyzer and activated by repeated liquid nitrogen freezing and thawing. The platelet-related cytokines were examined and analyzed by ELISA. SHEDs were extracted and cultured with different concentrations of mPRP or 10% FBS medium. Alkaline phosphatase (ALP) activity was measured. Mineralization factors, RUNX2 and OCN, were measured by real-time PCR. SHEDs were characterized with mesenchymal stem cells (MSCs) markers including vimentin, CD44, and CD105. mPRP at different concentrations (2, 5, 10, and 20%) enhanced the growth of SHEDs. Moreover, mPRP significantly stimulated ALP activity and promoted expression of RUNX2 and OCN compared with 10% FBS. mPRP could efficiently facilitate proliferation and differentiation of SHEDs, and 2% mPRP would be an optimal substitute for 10% FBS during SHEDs expansion and differentiation in clinical scale manufacturing.
Analysis of an ϵ-arbitrage model for incomplete markets

The optimal replication strategy for incomplete markets is obtained by solving a system of partial differential equations. In this paper, we study existence and uniqueness of the solution of this system and propose a numerical method to compute this optimal strategy. Resume. La strategie optimale de replication pour les marches incomplets est obtenue par la resolution d'un systeme d'´equations aux derivees partielles. Dans cet article, onetudie l'existence et l'unicite de la solution de ce systeme et on propose une methode numerique pour calculer cette strategie optimale.
Using jitter and shimmer in speaker verification

Jitter and shimmer are measures of the fundamental frequency and amplitude cycle-to-cycle variations, respectively. Both features have been largely used for the description of pathological voices, and since they characterise some aspects concerning particular voices, they are expected to have a certain degree of speaker specificity. In the current work, jitter and shimmer are successfully used in a speaker verification experiment. Moreover, both measures are combined with spectral and prosodic features using several types of normalisation and fusion techniques in order to obtain better verification results. The overall speaker verification system is also improved by using histogram equalisation as a normalisation technique previous to fusing the features by support vector machines.