Miracle-dz/UniFAR-FTUs huggingface dataset:
Cancer Targeting and Drug Delivery Using Carbon-Based Quantum Dots and Nanotubes

Currently cancer treatment is in large part non-specific with respect to treatment. Medication is often harsh on patients, whereby they suffer several undesired side effects as a result. Carbon-based nanoparticles have attracted attention in recent years due to their ability to act as a platform for the attachment of several drugs and/or ligands. Relatively simple models are often used in cancer research, wherein carbon nanoparticles are conjugated to a ligand that is specific to an overexpressed receptor for imaging and drug delivery in cancer treatment. These carbon nanoparticles confer unique properties to the imaging or delivery vehicle due to their nontoxic nature and their high fluorescence qualities. Chief among the ongoing research within carbon-based nanoparticles emerge carbon dots (C-dots) and carbon nanotubes (CNTs). In this review, the aforementioned carbon nanoparticles will be discussed in their use within doxorubicin and gemcitabine based drug delivery vehicles, as well as the ligand-mediated receptor specific targeted therapy. Further directions of research in current field are also discussed.
To Study the mechanical properties of coconut coir fiber reinforced with epoxy resin AW 106 & HV 953 IN

In this study the Mechanical Properties of Coconut coir Fiber is reinforced with epoxy resin of different lengths (5mm,10mm,15mm) and coir fibers by treating the fiber with NaOH by fraction of 5 % are studied here. All samples were made using hand layup technique and specimens were prepared as per ASTM standard D3039. The samples were tested according to ASTM D3039 standard using Universal testing machine (UTM). The significant findings of the research showed that NaOH treatment improved the tensile properties, ductility and hardness of the composite further Increase in length of fibers was found to increase the tensile strength. The maximum tensile strength was found for 15 mm length of coir fibers.
Contextualizing Hepatocyte Functionality of Cryopreserved HepaRG Cell Cultures

Over the last decade HepaRG cells have emerged as a promising alternative to primary human hepatocytes (PHH) and have been featured in over 300 research publications. Most of these reports employed freshly differentiated HepaRG cells that require time-consuming culture (∼28 days) for full differentiation. Recently, a cryopreserved, predifferentiated format of HepaRG cells (termed here “cryo-HepaRG”) has emerged as a new model that improves global availability and experimental flexibility; however, it is largely unknown whether HepaRG cells in this format fully retain their hepatic characteristics. Therefore, we systematically investigated the hepatocyte functionality of cryo-HepaRG cultures in context with the range of interindividual variation observed with PHH in both sandwich-culture and suspension formats. These evaluations uncovered a novel adaptation period for the cryo-HepaRG format and demonstrated the impact of extracellular matrix on cryo-HepaRG functionality. Pharmacologically important drug-metabolizing alleles were genotyped in HepaRG cells and poor metabolizer alleles for CYP2D6, CYP2C9, and CYP3A5 were identified and consistent with higher frequency alleles found in individuals of Caucasian decent. We observed liver enzyme inducibility with aryl hydrocarbon receptor, constitutive androstane receptor (CAR), and pregnane X receptor activators comparable to that of sandwich-cultured PHH. Finally, we show for the first time that cryo-HepaRG supports proper CAR cytosolic sequestration and translocation to hepatocyte nuclei in response to phenobarbital treatment. Taken together, these data reveal important considerations for the use of this cell model and demonstrate that cryo-HepaRG are suitable for metabolism and toxicology screening.
Retinal axon guidance at the midline: Chiasmatic misrouting and consequences

The visual representation of the outside world relies on the appropriate connectivity between the eyes and the brain. Retinal ganglion cells are the sole neurons that send an axon from the retina to the brain, and thus the guidance decisions of retinal axons en route to their targets in the brain shape the neural circuitry that forms the basis of vision. Here, we focus on the choice made by retinal axons to cross or avoid the midline at the optic chiasm. This decision allows each brain hemisphere to receive inputs from both eyes corresponding to the same visual hemifield, and is thus crucial for binocular vision. In achiasmatic conditions, all retinal axons from one eye project to the ipsilateral brain hemisphere. In albinism, abnormal guidance of retinal axons at the optic chiasm leads to a change in the ratio of contralateral and ipsilateral projections with the consequence that each brain hemisphere receives inputs primarily from the contralateral eye instead of an almost equal distribution from both eyes in humans. In both cases, this misrouting of retinal axons leads to reduced visual acuity and poor depth perception. While this defect has been known for decades, mouse genetics have led to a better understanding of the molecular mechanisms at play in retinal axon guidance and at the origin of the guidance defect in albinism. In addition, fMRI studies on humans have now confirmed the anatomical and functional consequences of axonal misrouting at the chiasm that were previously only assumed from animal models. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 844–860, 2017
Leadership style and organisational commitment among nursing staff in Saudi Arabia

AIMS To examine how nurse managers' leadership styles, and nurses' organisational commitment in Saudi Arabia relate. BACKGROUND Effective leadership is influential in staff retention; however, recruiting and maintaining nurses is an increasing problem in Saudi Arabia. METHODS Using a survey design, the Multifactor Leadership Questionnaire and the Organisational Commitment Questionnaire were distributed to a sample of 219 nurses and nurse managers from two hospitals in Saudi Arabia. RESULTS Transformational leadership was the most dominant leadership style. After controlling for the influence of manager/staff status, nationality and hospitals, transformational leadership was the strongest contributor to organisational commitment. Perceptions of both transformational and transactional leadership styles, increased with age for nurse managers and nursing staff. CONCLUSION Introducing the Full Range of Leadership model to the Saudi nursing workforce could help to prepare Saudi nurses for positions as nurse managers and leaders. IMPLICATIONS FOR NURSING MANAGEMENT The study provides insight into the type of leadership that is best suited to the dynamic and changing health care system in Saudi Arabia. It is possible that transformational leaders could influence and induce positive changes in nursing.
Prevalence and Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) Mastitis in Dairy Cattle in Jabalpur, Madhya Pradesh

Methicillin-resistant Staphylococcus aureus (MRSA) is a dreaded pathogen in human and veterinary medicine. MRSA as a cause of mastitis in dairy cattle has got profound economic and serious public health significance. A total of 111 dairy cattle were screened for mastitis by CMT from six dairy farms of Jabalpur. The 85 mastitis milk samples were screened for MRSA by bacterial culture method using cefoxitin for enrichment. The MRSA strains were identified and characterized by phenotypic and genotypic methods for virulence determinants and methicillin resistance. The MRSA strains showed β-haemolysis as a predominant haemolysis pattern. The strains were positive for mecA gene which is considered as a gold standard for the confirmation of methicillin resistance. The prevalence of MRSA mastitis in dairy cattle was 16.47%. The MRSA strains were positive for the virulence factors associated with pathogenicity. The economic and public health implication of the finding is discussed.
The let-7/Lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury

The let-7/Lin28 axis is associated with the regulation of key cellular regulatory genes known as microRNAs in various human disorders and cancer development. This study evaluated the role of the let-7/Lin28 axis in regulating a mesenchymal phenotype of hepatic stellate cells in alcoholic liver injury. We identified that ethanol feeding significantly down-regulated several members of the let-7 family in mouse liver, including let-7a and let-7b. Similarly, the treatment of human hepatic stellate cells (HSCs) with lipopolysaccharide (LPS) and transforming growth factor-β (TGF-β) significantly decreased the expressions of let-7a and let-7b. Conversely, overexpression of let-7a and let-7b suppressed the myofibroblastic activation of cultured human HSCs induced by LPS and TGF-β, as evidenced by repressed ACTA2 (α-actin 2), COL1A1 (collagen 1A1), TIMP1 (TIMP metallopeptidase inhibitor 1), and FN1 (fibronectin 1); this supports the notion that HSC activation is controlled by let-7. A combination of bioinformatics, dual-luciferase reporter assay, and Western blot analysis revealed that Lin28B and high-mobility group AT-hook (HMGA2) were the direct targets of let-7a and let-7b. Furthermore, Lin28B deficiency increased the expression of let-7a/let-7b as well as reduced HSC activation and liver fibrosis in mice with alcoholic liver injury. This feedback regulation of let-7 by Lin28B is verified in hepatic stellate cells isolated by laser capture microdissection from the model. The identification of the let-7/Lin28 axis as an important regulator of HSC activation as well as its upstream modulators and down-stream targets will provide insights into the involvement of altered microRNA expression in contributing to the pathogenesis of alcoholic liver fibrosis and novel therapeutic approaches for human alcoholic liver diseases.
Intervene to be Seen

Security cameras became such a part of everyday life that their presence may escape from our conscious attention. The present research examines the impact of cameras on intervening in crime, a situation in which the classic bystander effect has been uncovered. In our experimental set up, participants witnessed how another participant (a confederate) stole money, in the presence of either two or no other bystanders. Moreover, we used a security camera to make people feel watched. We expected to replicate the bystander effect without security camera’s presence and an attenuation of the bystander effect with a security camera present. As expected, the findings revealed that without a camera, participants were less likely to stop our confederate from stealing money when other bystanders were present. However, when there was a camera present this effect was attenuated: The camera increased intervention when people are otherwise least likely to help—when other bystanders are present.
Expressiveness in Flemish Online Teenage Talk: A Corpus-Based Analysis of Social and Medium-Related Linguistic Variation

We analyze linguistic expressiveness in an extensive corpus (2 million tokens) of Flemish online teenage talk, focusing on th e use of typographic chatspeak features, an onomatopoeic and a lexical variable and its correlation with the chatters’ profile and the online medium. General quantitative findings are that girls outperform boys in the expression of emotional involvement, and younger adolescents outperform the older group. However, medium has the largest impact: much more expressive markers are used in asynchronous social media posts than in synchronous instant messaging. On a qualitative level, utterances written by girls, by younger teenagers and on the asynchronous platform contain more expressive markers related to love or friendship. Apart from the medium’s (a)synchronicity and its public or private character, the nature of the interaction appears to be a determining factor too. The asynchronous social media posts involve a lot of flirting or pleasing, which drastically increases linguistic expressiveness.
Epidemiology and clinical outcomes in a multicentre regional cohort of patients with severe acquired brain injury

Abstract Purpose: To evaluate epidemiological and clinical data on patients with severe acquired brain injury (sABI) admitted to rehabilitation units in the first 6 years since the inception of a regional register (2005–2010) in the Emilia-Romagna region (Italy). Method: Retrospective multicentre study of a regional cohort using data from an online regional register (Gravi Cerebrolesioni Emilia-Romagna – GRACER). The study included 318 patients who suffered sABI (defined by Glasgow Coma Scale score ≤8 recorded in the initial 24 h following injury), who were admitted to and subsequently discharged from rehabilitation units. Physical and cognitive functions were evaluated at admission and discharge. Other data recorded included aetiology, presence of secondary conditions and need for specific medical support. Results: Three-quarters of patients displayed improvements in physical and/or cognitive function at discharge from rehabilitation units, with 71.4% of patients returning home. Better outcomes at discharge were associated in particular with younger age, traumatic brain injury (versus non-traumatic), or absence of tracheostomy at admission. Conclusion: The GRACER register is a useful tool for the assessment of epidemiological and clinical information on sABI patients. In light of the positive impact on patient outcomes, rehabilitation in specialised units is highly encouraged and should occur as soon as possible. Implications for Rehabilitation There is a need for more epidemiological and clinical data associated with severe acquired brain injury, particular regarding those of non-traumatic origin. In a retrospective multicentre study of a regional cohort using data from an online regional register in Italy (GRACER), more than three-quarters of patients displayed improvements in physical and/or cognitive function at discharge from the rehabilitation units. Better outcomes at discharge were associated in particular with younger age, traumatic brain injury (versus non-traumatic) or absence of tracheostomy at admission. Admission to a specialised rehabilitation unit is highly encouraged for patients with severe acquired brain injury, and should occur as soon as possible. Policy-makers and service planners should continue to develop strategies and allocate adequate resources for rehabilitation services due to their positive impact on patient outcomes. In particular, patients with conditions associated with increased likelihood of poor outcomes may require special attention during rehabilitation to improve outcomes at discharge.
[The Portuguese Guidelines and Patients Values].

INTRODUCTION Clinical guidelines are support tools, aiming to improve quality of the clinical practice. Patient centered care allows best satisfaction rates, with greater health self-management, and potential gains in quality with fewer costs. OBJECTIVE To evaluate the integration of patients' values in Portuguese guidelines. MATERIAL AND METHODS We reviewed the 18 guidelines about cardiovascular diseases published in Portugal from 2011 to 2013, searching for the integration of patients' ideas, fears, expectations and preferences. RESULTS Eight guidelines were related to diagnosis approach and 10 with treatment. We found references to patients' values in 5 (28%) guidelines, all about treatment aspects. The incorporation of patients' expectations was the most present feature. Reference to financial costs was present in 78% of the guidelines. DISCUSSION Clinical guidelines are health technology instruments available to practitioners in order to improve the quality of care provided to patients, who are the real users of these tools. As in other countries, there is a tendency to disregard the users' value systems in the conception of the guidelines, giving the privilege to a population logic, not always applicable to the individual, and to financial arithmetic with low support in cost-effectiveness assessments. In the Portuguese case, the way guidelines were proposed conditioned also some suspicion both in the professionals and in the users. CONCLUSION Portuguese guidelines have low incorporation of references to patients' values. This is more evident when questions about diagnosis are addressed, placing patients in a secondary role in the clinical decision-making process with potential losses in quality of care and eventual increase in costs.
In Vitro and In Vivo Antioxidant Activities of the Flowers and Leaves from Paeonia rockii and Identification of Their Antioxidant Constituents by UHPLC-ESI-HRMSn via Pre-Column DPPH Reaction

The genus Paeonia, also known as the “King of Flowers” in China, is an important source of traditional Chinese medicine (TCM). Plants of this genus have been used to treat a range of cardiovascular and gynecological diseases. However, the potential pharmacological activity of one particular species, Paeonia rockii, has not been fully investigated. In the first part of the present study, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), reducing power assays, and metal ion chelating assays were used to investigate the in vitro antioxidant activities of Paeonia rockii. In the second portion of the study, a mouse model of d-galactose-induced aging was used to validate the antioxidant effects of the flowers from Paeonia rockii in vivo. Lastly, potential antioxidant constituents were screened and identified by ultra-high pressure liquid chromatography and electrospray ionization coupled with high-resolution mass spectrometry (UHPLC-ESI-HRMSn) combined with the DPPH assay. Results indicated that the flowers and leaves exhibited stronger antioxidant activity than ascorbic acid in vitro. The therapeutic effect of Paeonia rockii was determined in relation to the levels of biochemical indicators, such as 8-iso-prostaglandin F2α (8-iso PGF2α) in the serum, superoxide dismutase (SOD), protein carbonyl, malondialdehyde (MDA), and glutathione (GSH) in the liver and brain, after daily intra-gastric administration of different concentrations of extracts (100, 200 and 400 mg/kg) for three weeks. The levels of 8-iso PGF2α (p < 0.01) and protein carbonyl groups (p < 0.01) were significantly reduced, whereas those of SOD (p < 0.05) had significantly increased, indicating that components of the flowers of Paeonia rockii had favorable antioxidant activities in vivo. Furthermore, UHPLC-ESI-HRMSn, combined with pre-column DPPH reaction, detected 25 potential antioxidant compounds. Of these, 18 compounds were tentatively identified, including 11 flavonoids, four phenolic acids, two tannins, and one monoterpene glycoside. This study concluded that the leaves and flowers from Paeonia rockii possess excellent antioxidant properties, highlighting their candidacy as “new” antioxidants, which can be utilized therapeutically to protect the body from diseases caused by oxidative stress.
Unified Theory for Decoding the Signals from X-Ray Florescence and X-Ray Diffraction of Mixtures

For research and development or for solving technical problems, we often need to know the chemical composition of an unknown mixture, which is coded and stored in the signals of its X-ray fluorescence (XRF) and X-ray diffraction (XRD). X-ray fluorescence gives chemical elements, whereas XRD gives chemical compounds. The major problem in XRF and XRD analyses is the complex matrix effect. The conventional technique to deal with the matrix effect is to construct empirical calibration lines with standards for each element or compound sought, which is tedious and time-consuming. A unified theory of quantitative XRF analysis is presented here. The idea is to cancel the matrix effect mathematically. It turns out that the decoding equation for quantitative XRF analysis is identical to that for quantitative XRD analysis although the physics of XRD and XRF are fundamentally different. The XRD work has been published and practiced worldwide. The unified theory derives a new intensity–concentration equation of XRF, which is free from the matrix effect and valid for a wide range of concentrations. The linear decoding equation establishes a constant slope for each element sought, hence eliminating the work on calibration lines. The simple linear decoding equation has been verified by 18 experiments.
Characterising the acoustic footprint of Australia's new research vessel RV Investigator

The RV Investigator is Australia’s new noise quietened Marine National Facility (MNF) vessel built to comply with the DNV silent-R specification. This radiated noise specification was based on the ICES 209 CRR specification designed with the main goal of minimising vessel avoidance for fisheries surveys. Noise quietening was specified for RV Investigator to improve the acoustic detection of instruments and reduce the vessel’s noise footprint on the environment for research activities. Traditionally DC propulsion motors are used to achieve radiated noise compliance; in this case AC propulsion motors have been used. Initial noise measurements show the RV Investigator is 20 dB (factor of 100) quieter than the previous MNF vessel, RV Southern Surveyor which will significantly improve acoustic instrument performance. The new acoustic systems on the RV Investigator range from sub-bottom profilers, Doppler current profilers, multi-frequency and multi-beam water-column echo-sounders and low to mid frequency multi-beam bathymetry echo-sounders.
Spatial information for resource management in Asia: a review of institutional issues

Abstract Although a number of Asian nations have acquired spatial information technology, limited use is made of this technology for promoting human welfare or resource management objectives. This technology is constrained not by technical but by social, economic and political issues. These include censorship, autonomy, rigid bureaucracies, sensitivity of land ownership data, shortage of appropriate training, cost and lack of public participation in resource decisions. Ideas suggested For overcoming these problems include valuing spatial information as an important resource, reforming the structure of government organizations, assessing and developing implementation tools, fitting the technology to the organization, encouraging incremental and user-driven development, and supporting new research and training programmes.
Efficacy of Nutritional Interventions on Inflammatory Markers in Haemodialysis Patients: A Systematic Review and Limited Meta-Analysis

Low-grade chronic inflammation is prevalent in patients undergoing haemodialysis (HD) treatment and is linked to the development of premature atherosclerosis and mortality. The non-pharmacological approach to treat inflammation in HD patients through nutritional intervention is well cited. We aimed to assess the efficacy of different nutritional interventions at improving inflammatory outcomes in HD patients, based on markers such as C-reactive protein (CRP), interleukin-6 (IL-6), and tumour necrosis factor-α (TNF-α). We searched PubMed, Cochrane Library, and Embase for randomized controlled trials (RCT) published before June 2017. Inclusion criteria included RCTs on adult patients on maintenance HD treatment with duration of nutritional interventions for a minimum 4 weeks. Risk of bias was assessed using the Jadad score. In total, 46 RCTs experimenting different nutritional interventions were included in the review and categorized into polyphenols rich foods, omega-3 fatty acids, antioxidants, vitamin D, fibres, and probiotics. Meta-analyses indicated significant reduction in CRP levels by omega-3 fatty acids (Random model effect: −0.667 mg/L, p < 0.001) and vitamin E (fixed model effect: −0.257 mg/L, p = 0.005). Evidence for other groups of nutritional interventions was inconclusive. In conclusion, our meta-analysis provided evidence that omega-3 fatty acids and vitamin E could improve inflammatory outcomes in HD patients.