Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrative Analysis of Proteome-wide Association Studies and Functional Enrichment Analysis to Identify Genes and Chemicals Associated with Alcohol Dependence

Objectives Alcohol dependence accounts for a large proportion of the global burden of disease and disability. This study aims to investigate the candidate genes and chemicals associated with alcohol dependence. Methods Using data from published alcohol dependence genome-wide association studies, we first conducted a proteome-wide association study of alcohol dependence by integrating alcohol dependence genome-wide association studies with 2 human brain reference proteomes of dorsolateral prefrontal cortex from the Religious Order Study and Rush Memory and Aging Project and the Banner Sun Health Research Institute. Then, based on the identified genes in proteome-wide association study, we conducted functional enrichment analysis and chemical-related functional enrichment analysis to detect the related Gene Ontology terms and chemicals. Results Proteome-wide association study identified several potential candidate genes for alcohol dependence, such as GOT2 (P = 7.59 × 10−6) and C3orf33 (P = 5.00 × 10−3). Furthermore, functional enrichment analysis identified multiple candidate Gene Ontology terms associated with alcohol dependence, such as glyoxylate metabolic process (adjusted P = 2.99 × 10−6) and oxoglutarate metabolic process (adjusted P = 9.95 × 10−6). Chemical-related functional enrichment analysis detected several alcohol dependence–related candidate chemicals, such as pitavastatin (P = 2.00 × 10−4), cannabinoids (P = 4.00 × 10−4), 11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid (P = 4.00 × 10−4), and gabapentin (P = 2.00 × 10−3). Conclusions Our study reports multiple candidate genes and chemicals associated with alcohol dependence, providing novel clues for understanding the biological mechanism of alcohol dependence.
Decisional Regret Among Caregivers of Infants with Differences of Sex Development Reared as Male

Abstract: Objective: Differences of sex development (DSD) are congenital conditions in which individuals are discordant in their chromosomal, phenotypic, and/or gonadal sex. Treatment of DSD can involve surgical intervention to external genitalia to make anatomy seem male-typical (i.e., male genitoplasty). Caregiver-perceived decisional regret regarding young boys with DSD was explored quantitatively and qualitatively. Method: Participants (N = 39) were caregivers of infants (N = 23) diagnosed with DSD (mean age = 8.9 months, standard deviation = 5.9 months) reared male participating in a longitudinal investigation of psychosocial outcomes. Qualitative data were collected at 6 to 12 months after baseline enrollment to evaluate caregiver decision-making corresponding to levels of regret concerning their child's treatment. All but one infant received genital surgery before caregiver reporting on their decisional regret. Quantitative exploratory analyses evaluated longitudinal predictors of decisional regret at 6 to 12 months. Results: When completing a write-in item inquiring about decision-making and potential regret, most caregivers (n = 16, 76%) reported that their child's genital surgery was their first medical decision. Two caregivers referenced gender assignment as a decision point. One-third of caregivers reported some level of decisional regret (33%), with 67% reporting no regret. No hypothesized predictors of decisional regret were statistically significant. Conclusion: Many caregivers of infants with DSD reared male view genital surgery as a first health care decision. Approximately one-third of caregivers reported some level of decisional regret. Further research is warranted to explore long-term decisional regret; it will be particularly important to investigate the decisional regret of patients with DSD.
Organellar DNA continues to provide a rich source of information in the genomics era

The genomics revolution continues to change how ecologists and evolutionary biologists study the evolution and maintenance of biodiversity. It is now easier than ever to generate large molecular data sets consisting of hundreds to thousands of independently evolving nuclear loci to estimate a suite of evolutionary and demographic parameters. However, any inferences will be incomplete or inaccurate if incorrect taxonomic identities and perpetuated throughout the analytical pipeline. Due to decades of research and comprehensive online databases, sequencing and analysis of mitochondrial DNA (mtDNA), chloroplast DNA (cpDNA) and select nuclear genes can provide researchers with a cost effective and simple means to verify the species identity of samples prior to subsequent phylogeographic and population genomic analysis. The addition of these sequences to genomic studies can also shed light on other important evolutionary questions such as explanations for gene tree‐species tree discordance, species limits, sex‐biased dispersal patterns, adaptation, and mtDNA introgression. Although the mtDNA and cpDNA genomes often should not be used exclusively to make historical inferences given their well‐known limitations, the addition of these data to modern genomic studies adds little cost and effort while simultaneously providing a wealth of useful data that can have significant implications for both basic and applied research.
The centrality of work in everyday life after stroke: A qualitative study of long-term stroke survivors

Abstract Background Work is an occupation of great concern for younger stroke survivors. Given the high rate of people not working after stroke, there is a need to explore work after stroke from a long-term perspective, including not just an initial return to work, but also the ability to retain employment and how this may affect everyday life after stroke. Therefore, the objective of this study was to explore experiences relating to work and to work incapacity among long-term stroke survivors. Method This study used thematic analysis on data gathered through individual semi-structured interviews with long-term stroke survivors. Results The analysis resulted in four themes that together comprised the main theme ‘The centrality of work in everyday life’, containing descriptions of how everyday life was affected by aspects of work both for those who did work and those who did not return to work after stroke. Conclusion and significance The results highlight the importance of addressing return to work not just as an isolated outcome but as part of everyday life after stroke. The results indicate a need for a more flexible approach to supporting return to work that continues past the initial return.
Radio frequency current generation by waves in toroidal geometry

The quasilinear generation of current by rf wave fields is investigated in toroidal geometry. A general expression is derived for the amount of current generated for a given wave‐induced flux of particles. This expression should be of great practical value in assessing the various competing effects on current generation in toroidal geometry and is well‐suited for implementation in a toroidal ray tracing code.
Be prepared and do the best you can: a focus group study with staff on the care environment at Swedish secure youth homes

ABSTRACT This study focuses on the care environment of secure youth homes run by the Swedish National Board of Institutional Care, where youths up to 21 years old are placed according to law due to extensive care needs or sentences. Purpose This study examined staff members’ experiences of the institutional care environment within secure youth homes. Methods Data were collected through three focus group discussions with 17 staff members at two secure youth homes. Subsequently, a thematic analysis was conducted. Results The analysis indicated two main themes: risk management and damage control in a restricted environment and compensating and reconstructing ordinariness—trying to make the best of it; each theme had three subthemes. The care environment seems to be experienced by staff as characterized by conflicting demands, thus constituting a gap between needs and what is possible to achieve—a balancing act that constitutes a constant struggle. Conclusions The staff members’ constant struggle could be interpreted as conflicting moral and instrumental demands; they know what the youths need, but the environment of the secure youth homes demands the decorous behaviour of sociomaterial control practices—rather than care practices.
Patients are pragmatic and physicians are perfectionists? Comparing success after liver transplantation among patients and physicians

Although both patients and physicians are key stakeholders in health care outcomes, patients and physicians often define success differently. The purpose of this study was to compare patient and physician perceptions of success 1 year after liver transplantation. This was a single-institution, qualitative study. We conducted in-person, semi-structured interviews with liver transplant recipients 1 year after transplantation and virtual interviews with transplant surgeons and hepatologists. Transcripts were coded and iteratively analyzed for themes using the principles of phenomenology. Twenty patients, 8 caregivers, 5 transplant surgeons, and 4 hepatologists were interviewed. Subject interviews averaged 57 (patient) and 27 (physician) minutes. Overall, patients and physicians had significant agreement in their definitions of success, which included avoidance of death, restoration of physical and mental function, return to society, acquisition of new health care knowledge, and open communication between the patient and the physician. Patients highlighted relief from worry about their future health status, and physicians highlighted decreased health care costs. Patients noted that a liver transplant did not have to be perfect, that is free from complications, to be successful. Physicians had a more stringent view and felt that any deviation from an ideal course reduced the relative success of a transplant. Detailed assessment of patient and physician responses reveals similar overall goals of regaining physical, mental, and emotional function. Complications are perceived differently by patients and physicians. Awareness of this discordance may serve to enhance relationships between transplant patients and their providers.
Prevalence and Etiologies of Renal and Urinary Manifestations in a Large Cohort of Children With Inflammatory Bowel Disease

Background and objectives: Renal and/or urinary manifestations (RUM) have been reported in pediatric patients with inflammatory bowel disease (IBD) but their incidence is unknown. The aims of this study were to assess the prevalence and causes of these manifestations in children with IBD and determine the causal link with 5-aminosalicylic acid (5-ASA) treatment. Methods: A retrospective observational study was performed with children with diagnosis of IBD. All children with RUM during follow-up and/or impaired renal function [estimated glomerular filtration rate (eGFR) < 90 mL/min/1.73 m2] were identified. Results: Of 228 included patients, 9 (3.9%) had a RUM during follow-up [follow-up: 5 years (1–12 years)] at a median age of 16 years (8–17 years). It concerned 7 of 171 patients with Crohn disease and 2 of 57 with ulcerative colitis. Seven patients were taking 5-ASA at the time of the RUM. Only 1 of them had an iatrogenic renal complication related to this treatment. Patients with RUM had a more severe disease with increased anti-tumor necrosis factor-α use (P = 0.031), more abscesses (P = 0.003), and a higher rate of digestive surgery (P = 0.04). For the whole cohort, a significant decrease in eGFR was found during follow-up (121 vs 107 mL/min/1.73 m2, P < 0.001). At the end of follow-up, 38 of 202 (19%) patients had an eGFR < 90 mL/min/1.73 m2. Conclusion: In children with IBD, RUM can occur, independently of treatment with 5-ASA. During follow-up, a significant decrease in eGFR was observed. We suggest monitoring renal function in all patients with IBD.
Barriers to conducting independent quantitative research in low-income countries: A cross-sectional study of public health graduate students in Liberia

Introduction During recent disease outbreaks, quantitative research has been used to investigate intervention scenarios while accounting for local epidemiological, social, and clinical context. Despite the value of such work, few documented research efforts have been observed to originate from low-income countries. This study aimed to assess barriers that may be limiting the awareness and conduct of quantitative research among Liberian public health graduate students. Methods A semi-structured questionnaire was administered September-November 2021 to Master’s in Public Health (MPH) students in Liberia. Potential barriers around technology access, understanding of quantitative science, and availability of mentorship were interrogated. Associations between barriers and self-reported likelihood of conducting quantitative research within six months of the investigation period were evaluated using ordinal logistic regression. Results Among 120 participating MPH students, 86% reported owning a personal computer, but 18.4% and 39.4% had machines with malfunctioning hardware and/or with battery power lasting ≤2 hours, respectively. On average, students reported having poor internet network 3.4 days weekly. 47% reported never using any computer software for analysis, and 46% reported no specific knowledge on statistical analysis. Students indicated spending a median 30 minutes per week reading scientific articles. Moreover, 50% had no access to quantitative research mentors. Despite barriers, 59% indicated they were very likely to undertake quantitative research in the next 6 months; only 7% indicated they were not at all likely. Computer ownership was found to be statistically significantly associated with higher likelihood of conducting quantitative research in the multivariable analysis (aOR: 4.90,95% CI: 1.54–16.3). Conclusion The high likelihood of conducting quantitative research among MPH students contrasts with limitations around computing capacity, awareness of research tools/methods, and access to mentorship. To promote rigorous analytical research in Liberia, there is a need for systematic measures to enhance capacity for diverse quantitative methods through efforts sensitive to the local research environment.
Prevalence of intestinal parasites in animal hosts and potential implications to animal and human health in Edo, Nigeria

Background Intestinal parasites in livestock cause huge economic setbacks. Moreover, these parasites can threaten human health when also present in companion animals. Objectives The study examined the prevalence and burden of intestinal parasites among roaming/migrating animals (goats, sheep, cattle, and dogs) to provide insights into the risk of potential human parasitic infections. Methods A total of 1,741 fecal samples from goats (n = 920), sheep (n = 335), cattle (n = 230) and dogs (n = 256) were obtained randomly across 18 local government areas in Edo State, Nigeria. The parasite samples were recovered and identified under a microscope. Molecular tools were used to identify Toxocara spp. Results Eighteen different parasites were isolated. Among the different groups of parasites observed, nematodes occurred the most, followed by protozoans. Among nematodes, Haemonchus was most prevalent in goats (28.04%) and sheep (29.85%), while Strongyloides (10.86%) and Bunostomum (8.69%) were relatively high in cattle. Strongyloides (3.9%), hookworm (3.9%), and Toxocara (3.12%) were the predominant parasites in dogs. For protozoan parasites, Eimeria was most common in all 4 animal hosts. Several goats (2.39%) and sheep (2.38%) tested positive for Fasciola spp. Molecular analysis confirmed Toxocara canis in dogs for the first time in Nigeria. Conclusions The major parasites recovered from these roaming/migrating animals have zoonotic potentials that can threaten human health.
Beneficial Performance Effects of Training Load Intensification Can Be Abolished by Functional Overreaching: Lessons From a Water Polo Study in Female Athletes

Abstract Brisola, GMP, Dutra, YM, Murias, JM, and Zagatto, AM. Beneficial performance effects of training load intensification can be abolished by functional overreaching: Lessons from a water polo study in female athletes. J Strength Cond Res 37(6): e391–e402, 2023—The purpose of this study was to compare the outcomes from 2 weeks of training load intensification strategy in female water polo players diagnosed with functional overreaching (F-OR) with no F-OR players (acute fatigue) on the performance outcomes and hormonal, immunological, and cardiac autonomic nervous system responses. Twenty-two female water polo players were allocated into control and intensification group during 7 weeks. The swimming performance, biochemical parameters, heart rate variability, profile of mood states, and upper respiratory tract infection symptoms were assessed twice before and twice after 2 weeks of intensification period. F-OR showed a worsening in total time of the repeated sprint ability (RSA) test compared with the control group and the acute fatigue group after intensification (p ≤ 0.035). Furthermore, after the tapering period, the F-OR group maintained worse total time of the RSA test than the acute fatigue group (p = 0.029). In addition, the acute fatigue group showed improvement in total time of the RSA test after intensification compared with the control group (p < 0.001). No significant interactions were found for the other parameters. Therefore, periods of intensification without the F-OR development can promote higher gains in the total time of the RSA test after intensification and tapering period.
Development and Validation of a Hypertension Risk Calculator for Living Kidney Donors

Background. Ideally, when deciding whether to donate, kidney donor candidates would understand their long-term risks. Using single-center data (N = 4055; median [quartiles] follow-up: 18 [9–28] y), we developed a calculator for postdonation hypertension and validated it using long-term data from an external single-center cohort (N = 1189, median [quartiles] follow-up: 9 [5–17] y). Methods. Risk factors considered were routinely obtained at evaluation from donor candidates. Two modeling approaches were evaluated: Cox proportional hazards and random survival forest models. Cross-validation prediction error and Harrell’s concordance-index were used to compare accuracy for model development. Top-performing models were assessed in the validation cohort using the concordance-index and net reclassification improvement. Results. In the development cohort, 34% reported hypertension at a median (quartiles) of 16 (8–24) y postdonation; and in the validation cohort, 29% reported hypertension after 17 (10–22) y postdonation. The most accurate model was a Cox proportional hazards model with age, sex, race, estimated glomerular filtration rate, systolic and diastolic blood pressure, body mass index, glucose, smoking history, family history of hypertension, relationship with recipient, and hyperlipidemia (concordance-index, 0.72 in the development cohort and 0.82 in the validation cohort). Conclusions. A postdonation hypertension calculator was developed and validated; it provides kidney donor candidates, their family, and care team a long-term projection of hypertension risk that can be incorporated into the informed consent process.
Effect of 12-Week-Zumba Training on Postural Balance, Lower Limb Strength, Mood and Quality of Life in Postmenopausal Women

ABSTRACT We aimed to investigate 12-week-Zumba training effects on physical and psychological parameters, and quality of life (QoL) in postmenopausal women. Forty-two postmenopausal women were randomly allocated to a control group or a Zumba group (ZG). Postural balance, lower limb strength, mood level, and QoL were assessed before and after the 12-week-Zumba training. The ZG showed significantly better balance performances under all conditions such as on firm and foam surfaces with opened and closed eyes as well as improvements in limb strength, mood and QoL compared to their baselines. Thereby, 12-week-Zumba training was effective in improving postural balance, limb strength, mood and, QoL in postmenopausal women.
MIRN: A multi-interest retrieval network with sequence-to-interest EM routing

Vector-based retrieval have been widely adopted to process online users’ diverse interests for recommendations. However, most of them utilize a single vector to represent user multiple interests (UMI), inevitably impairing the accuracy and diversity of item retrieval. In addition, existing work often does not take into account the scale and speed of the model, and high-dimensional user representation vectors need high computation cost, leading to inefficient item retrieval. In this paper, we propose a novel lightweight multi-interest retrieval network (MIRN) by incorporating sequence-to-interest Expectation Maximization (EM) routing to deal with users’ multiple interests. By leveraging representation ability of the Capsule network, we design a multi-interest representation learning module that clusters multiple Capsule vectors from the user’s behavior sequence to represent each of their interests respectively. In addition, we introduce a composite capsule clustering strategy for the Capsule network framework to reduce the scale of the network model. Furthermore, a Capsule-aware module incorporating an attention mechanism has been developed to guide model training by adaptively learning multiple Capsule vectors of user representations. The experimental results demonstrate MIRN outperforms the state-of-the-art approaches for item retrieval and gains significant improvements in terms of metric evaluations.
Gastric dysmotility and gastrointestinal symptoms in myalgic encephalomyelitis/chronic fatigue syndrome

Abstract Background Gastrointestinal symptoms are common in ME/CFS, but there is a knowledge gap in the literature concerning gastrointestinal motility features and detailed symptom description. Objective In this study, we aimed to characterize gastric motility and gastric symptoms in response to a liquid meal. Methods We included 20 patients with ME/CFS with abdominal complaints who were recruited to a double-blind randomized placebo-controlled trial of Rituximab. The patients of this sub study were examined with an ultrasound drink test, and gastrointestinal symptoms were evaluated using the Rome III questionnaire and Irritable Bowel Syndrome Symptom Severity Scale (IBS-SSS) questionnaire. Results We found that patients commonly reported fullness/bloating (75%), abdominal pain (45%) and nausea (35%). Ultrasound measurements revealed lower proximal measurements of the stomach after a meal (p < 0.01) and larger fasting antral area (p = 0.019) compared to healthy controls. The patients had a stronger symptomatic response to the liquid meal compared to healthy controls regarding epigastric pain, discomfort and nausea (p < 0.05). Ninety percent of the patients reported bowel movement frequencies within the normal range but scored high on bowel habit dissatisfaction and life disruption. Conclusion The patients presented with fullness/bloating, nausea and epigastric pain, showed signs of impaired gastric accommodation and visceral hypersensitivity, showing that the gastrointestinal symptoms of ME/CFS patients are similar to functional dyspepsia. Key summary Gastrointestinal symptoms are common in ME/CFS, but there is a knowledge gap in the literature concerning gastrointestinal motility features and detailed symptom description. • In this study, patients with ME/CFS had signs of impaired gastric accommodation after a liquid meal. • Out of 20 patients, 15 patients reported fullness/bloating, 9 reported abdominal pain, and 7 reported nausea. The patients showed signs of visceral hypersensitivity on a drink test. • Our findings suggest that patients with ME/CFS share many similarities with patients with Functional Dyspepsia. The findings were not typical for Irritable Bowel Syndrome.