Miracle-dz/UniFAR-FTUs huggingface dataset:
Investigation of Momordica charantia phytochemicals against PIM1 Kinase: A Computational Approach

Prostate cancer is a significant contributor to male cancer-related mortality. PIM1 kinase has implications in the development and progression of various cancers, particularly prostate cancer. PIM1, a serine/threonine protein kinase plays a crucial role in cellular processes including survival, growth and differentiation. In prostate cancer increased PIM1 expression is associated with a more aggressive phenotype and poorer patient outcomes. It has emerged as a promising therapeutic target for prostate cancer treatment. The development of PIM1 kinase inhibitors has greatly enhanced and progressed significantly. Different stages of clinical trials demonstrating their potential as therapeutic agents. Momordica charantia, or bitter melon has a long history in traditional medicine for various health conditions, it is very rich in secondary metabolites like triterpenoids, glycosides, alkaloids, flavonoids and phenolic acids. Bitter melon is considered to have medicinal properties including potential anticancer phytochemicals. This study employs virtual screening, molecular dynamic simulation and ADME/T analysis to explore bitter melon’s phytochemicals and their interaction with PIM1 kinase. The goal is to understand the molecular details and pharmacokinetics of bitter melon compounds evaluating their potential as therapeutic agents against prostate cancer. In our present study, it was found that out of all investigated phytochemicals catechin and gallic acid shows satisfactory result depending upon various parameters taken into consideration for conducting the study.
Computational analysis of cas proteins unlocks new potential in HIV-1 targeted gene therapy

Introduction: The human immunodeficiency virus type 1 (HIV-1) pandemic has been slowed with the advent of anti-retroviral therapy (ART). However, ART is not a cure and as such has pushed the disease into a chronic infection. One potential cure strategy that has shown promise is the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas gene editing system. It has recently been shown to successfully edit and/or excise the integrated provirus from infected cells and inhibit HIV-1 in vitro, ex vivo, and in vivo. These studies have primarily been conducted with SpCas9 or SaCas9. However, additional Cas proteins are discovered regularly and modifications to these known proteins are being engineered. The alternative Cas molecules have different requirements for protospacer adjacent motifs (PAMs) which impact the possible targetable regions of HIV-1. Other modifications to the Cas protein or gRNA handle impact the tolerance for mismatches between gRNA and the target. While reducing off-target risk, this impacts the ability to fully account for HIV-1 genetic variability. Methods: This manuscript strives to examine these parameter choices using a computational approach for surveying the suitability of a Cas editor for HIV-1 gene editing. The Nominate, Diversify, Narrow, Filter (NDNF) pipeline measures the safety, broadness, and effectiveness of a pool of potential gRNAs for any PAM. This technique was used to evaluate 46 different potential Cas editors for their HIV therapeutic potential. Results: Our examination revealed that broader PAMs that improve the targeting potential of editors like SaCas9 and LbCas12a have larger pools of useful gRNAs, while broader PAMs reduced the pool of useful SpCas9 gRNAs yet increased the breadth of targetable locations. Investigation of the mismatch tolerance of Cas editors indicates a 2-missmatch tolerance is an ideal balance between on-target sensitivity and off-target specificity. Of all of the Cas editors examined, SpCas-NG and SPRY-Cas9 had the highest number of overall safe, broad, and effective gRNAs against HIV. Discussion: Currently, larger proteins and wider PAMs lead to better targeting capacity. This implies that research should either be targeted towards delivering longer payloads or towards increasing the breadth of currently available small Cas editors. With the discovery and adoption of additional Cas editors, it is important for researchers in the HIV-1 gene editing field to explore the wider world of Cas editors.
Changes in Meat of Hu Sheep during Postmortem Aging Based on ACQUITY UPLC I-Class Plus/VION IMS QTof

Meat and meat products have a critical role in the human diet as important high-nutrient foods that are widely consumed worldwide. This study evaluated the effects of postmortem aging on Hu sheep’s meat quality in the longissimus dorsi (LD) muscle during postmortem aging. The samples were stored at 4 ± 1 °C; the meat quality was measured at 6 h, 12 h, 24 h, 36 h, 48 h, 72 h, 96 h, 120 h, 144 h, and 168 h of postmortem aging. The results showed that, during the postmortem aging process, the pH of the muscles first decreased and then increased, and the shear force first increased and then decreased. The muscle fiber skeleton began to degrade, and the overall meat quality was improved to some extent. In addition, through ACQUITY UPLC I-Class Plus IMS Qtof identification of the muscle samples at different time points during the postmortem maturation process of the meat of Hu sheep, a total of 2168 metabolites were identified, and 470 metabolites were screened based on the VIP, P, and FC values, of which 79 were involved in KEGG pathways. In addition, pathways such as sphingolipid metabolism, glycerophospholipid metabolism, phenylalanine metabolism, and fatty acid elongation and degradation play an important role in the metabolic product changes in the meat of Hu sheep throughout the entire maturation process. These findings provide some insights into the changes in meat quality during the post-slaughter maturation process of lake lamb.
Blocking of microglia-astrocyte proinflammatory signaling is beneficial following stroke

Microglia and astrocytes play an important role in the neuroinflammatory response and contribute to both the destruction of neighboring tissue as well as the resolution of inflammation following stroke. These reactive glial cells are highly heterogeneous at both the transcriptomic and functional level. Depending upon the stimulus, microglia and astrocytes mount a complex, and specific response composed of distinct microglial and astrocyte substates. These substates ultimately drive the landscape of the initiation and recovery from the adverse stimulus. In one state, inflammation- and damage-induced microglia release tumor necrosis factor (TNF), interleukin 1α (IL1α), and complement component 1q (C1q), together “TIC. ” This cocktail of cytokines drives astrocytes into a neurotoxic reactive astrocyte (nRA) substate. This nRA substate is associated with loss of many physiological astrocyte functions (e.g., synapse formation and maturation, phagocytosis, among others), as well as a gain-of-function release of neurotoxic long-chain fatty acids which kill neighboring cells. Here we report that transgenic removal of TIC led to reduction of gliosis, infarct expansion, and worsened functional deficits in the acute and delayed stages following stroke. Our results suggest that TIC cytokines, and likely nRAs play an important role that may maintain neuroinflammation and inhibit functional motor recovery after ischemic stroke. This is the first report that this paradigm is relevant in stroke and that therapies against nRAs may be a novel means to treat patients. Since nRAs are evolutionarily conserved from rodents to humans and present in multiple neurodegenerative diseases and injuries, further identification of mechanistic role of nRAs will lead to a better understanding of the neuroinflammatory response and the development of new therapies.
Unconventional Jamming Scheme for Multiple Quadrature Amplitude Modulations

It is generally believed that jamming signals similar to communication signals tend to demonstrate better jamming effects. We believe that the above conclusion only works in certain situations. To select the correct jamming scheme for a multi-level quadrature amplitude modulation (MQAM) signal in a complex environment, an optimal jamming method based on orthogonal decomposition (OD) is proposed. The method solves the jamming problem from the perspective of the in-phase dimension and quadrature dimension and exhibits a better jamming effect than normal methods. The method can construct various unconventional jamming schemes to cope with a complex environment and verify the existing jamming schemes. The Experimental results demonstrate that when the jammer ideally knows the received power at the receiver, the proposed method will always have the optimal jamming effects, and the constructed unconventional jamming scheme has an excellent jamming effect compared with normal schemes in the case of a constellation distortion. key words: multi-level quadrature amplitude modulation, orthogonal decomposition, optimal jamming, in-phase, quadrature phase, constellation distortion
Quantitative Assessment Characteristics of Small Pulmonary Vessel Remodelling in Populations at High Risk for COPD and Smokers Using Low-Dose CT

Purpose To explore the morphological alterations in small pulmonary vessels in populations at high risk for chronic obstructive pulmonary disease (COPD) and smokers based on multiple computed tomography (CT) quantitative parameters. Patients and Methods A total of 1969 Three Major Chest Diseases Screening Study participants with available demographic data and smoking history who underwent low-dose chest CT from 2018 to 2020 were included. All subjects were divided into normal, high risk for COPD, and COPD groups according to their pulmonary function test (PFT) results. Furthermore, the three groups were further subdivided into never-smokers, current smokers, and former smokers subgroups according to their smoking history. Quantitative parameters, such as the number, area at 6 mm~24 mm subpleura and volume of small pulmonary vessels, were extracted by computer software. Differences in small pulmonary vessel parameters among the groups were compared using two-way ANOVA. Results The number, area at 6 mm~24 mm subpleura and volume of small pulmonary vessels in the group at high risk for COPD were lower than those in the normal group (P<0.05). The number, area at 6 mm~24 mm subpleura and volume of small pulmonary vessels in the COPD group were higher than those in the normal group (P<0.05). The number, area of small pulmonary vessels at 6 mm~12 mm subpleura in current smokers with high risk for COPD were higher than those in former smokers with high risk for COPD (P<0.05). Conclusion The number, area, and volume of small pulmonary vessels in populations at high risk for COPD were decreased. Smoking cessation may impede structural changes in small pulmonary vessels in populations at high risk for COPD.
Proximal chemical analysis and secondary metabolites in Washingtonia robusta fruit (Arecaceae): relevance for the feeding of wildlife and human

<p><strong>Background:</strong> <em>Washingtonia robusta</em> fruits are a staple for birds, small and medium-size mammals, and humans. However, quantitative data of their nutritional contribution has remained unknown. This is the first report on the phytochemical components found in fruit pulp and seeds. </p><p><strong>Questions:</strong><em> </em>Does pulp compounds favor its consumption and contribute with nutrients for wildlife and humans? Do seed contents favor ecological interactions for germination and plantlet development? </p><p><strong>Species study:</strong><em> Washingtonia robusta</em> H. Wendl. </p><p><strong>Study site and dates:</strong><em> </em>Sierra El Aguaje, Sonora, Mexico, February - April 2016</p><p><strong>Methods:</strong><em> </em>Chemical and secondary metabolite analyses were carried out from extracts via phytochemical coloring tests and ultra-resolution liquid chromatography. Antioxidant activity was analyzed by DPPH. </p><p><strong>Results:</strong><em> Washingtonia robusta</em> seeds contain 73 % sugars, 7.4 % protein, 8.4 % humidity, 4.3 % ashes, 8.7 % fat and 0.2 %<em> </em>calcium, while cyanogenic glycosides content was 0.8 μg/g. Pulp has 71 % sugars, 10.8 % protein, 1.6 % humidity, 5.5 % ashes, 9.4 % fat and 1.5 % calcium, and cyanogenic glycosides reached 0.2 μg/g. Alkaloids, tannins, saponins, phenols, and flavonoids were more abundant in seeds than in pulp. The DPPH assay expressed in Trolox equivalents indicated antioxidant capacity. <em></em></p><strong>Conclusions:</strong><em> Washingtonia robusta </em>pulp is an important source of sugars and natural antioxidants for wildlife and human consumption. Occurrence of secondary metabolites prevents pathogens in seeds, also they could be beneficial for germination and initial plantlet development.
Usability Study to Promote Co-Creation Among People With Disabilities, Developers, and Makers With a Focus on the Assistive Technology Open Platform in Korea

Assistive technology plays an important role in facilitating independent living and a high quality of life for older adults and people with disabilities, who depend on the provision of customized assistive devices for various physical functions. Technological advances such as 3D printers have facilitated responses to some of these needs; however, the lack of relevant policies, information, and services makes proper use of these facilities difficult. Therefore, the National Rehabilitation Center established the AT Open Platform—an open-source sharing platform for AT. The AT Open Platform revitalized the customized assistive device industry by using appropriate advanced technologies. In the AT Open Platform, older adults and those with disabilities propose needs, developers develop solutions for these needs and share products as open sources, and manufacturers provide services for older adults and those with disabilities using open sources. Having these various stakeholders interacting on one platform requires high usability. In this study, the usability of open platforms was evaluated by categorizing each stakeholder (idea proposer, developer, and maker); efficiency was evaluated by thematic think-aloud analysis; effectiveness was evaluated by task analysis; and satisfaction was evaluated using the System Usability Scale and the e-government website UI/UX quality evaluation table. Additionally, the functions, content, and visual designs of the open platforms were evaluated. Through this investigation, we suggested ways to improve open-source assistive device websites to meet the needs of each stakeholder. We expect that this open platform with improved usability will improve user accessibility and make it easier to find and use assistive devices that are essential in daily life.
Spatial and temporal heterogeneity of macrofungi in the protected forests of Southern India

Macrofungal inventory at fortnightly intervals up to 10 weeks in two protected forests (arboretum and botanical garden) of the Southwestern India during southwest monsoon yielded 53 species belong to 33 genera. A total of 29 (22 genera) and 36 (26 genera) species were recovered in arboretum and botanical garden, respectively. Sporocarp richness was higher in botanical garden than in arboretum (742 vs. 684). Richness of species, genera, sporocarps and diversity attained the highest during 4th wk except for sporocarps in arboretum (2nd wk). The overall Sorensen's similarity was 36.9% between habitats, while between fortnights of habitats ranged from 18.2% (8th wk) to 34.8% (2nd wk). Both habitats were dominated by four species without overlap. The highest number of fungi was obtained on soil in botanical garden (29.3%), woody debris in arboretum (18.5%) and four species occurred on two substrates. Species richness was higher in medium and coarse than in fine woody debris. Among the abiotic factors, depth of leaf litter, soil moisture, soil pH and total phosphorus content of soil were significantly differed between habitats (P < 0.05). In arboretum, species richness vs. soil conductivity showed a positive correlation (R = 0.745), while species richness vs. soil phosphorus content was negatively correlated (R =  0.747). Nearly 43% of macrofungi in this survey have economic value as edible (12 spp.), medicinal (7 spp.) and mycorrhizal (10 spp.). Up to 7 species have duel benefit as edible/medicinal (1 sp.), edible/mycorrhizal (4 spp.) and medicinal/mycorrhizal (2 spp.). Results of this study advocate future prospects of forest management in the Southwestern India in favor of production of macrofungi during southwest monsoon.
Expression of microRNAs ‘let-7d and miR-195’ and Apoptotic Genes ‘BCL2 and Caspase-3’ as Potential Biomarkers of Female Breast Carcinogenesis

Objective: Breast cancer (BC) is the most common cause of cancer-related death among women worldwide. Let-7d and microRNA-195 (miR-195) are members of microRNAs that are known tumor suppressors and are involved in the regulation of apoptosis, invasion, and other cellular functions. However, the roles of these microRNAs in BC progression remain controversial. This study aimed to explore the correlation between the expression of let-7d and miR-195 and apoptosis-related genes (ARGs) “B-cell lymphoma 2 (BCL2) and caspase-3 (CASP3)” as potential biomarkers of breast carcinogenesis. Methods: It was a retrospective case-control study in which expression of let-7d, miR-195, CASP3, and BCL2 was assessed using quantitative real-time PCR (qRT-PCR); and immunohistochemical (IHC) staining was used to determine expression of BCL2 and CASP3 in BC tissue versus normal breast tissue (NT) samples. Results: The expression of let-7d and miR-195 was significantly reduced within BC tissues compared to NT (P: < 0.0001); and there was a statically positive correlation between them (r=0.314, P: 0.005). They have also been correlated to biomarkers’ expression of genes related to apoptosis. There was a statistically significant positive association between CASP3, and both let-7d, and miR-195 relative gene expression (r=0.713, P: <0.0001 and r=0.236, P: 0.03, respectively). In contrast, there was a statistically significant negative association between the relative gene expression of BCL2, with let-7d, and miR-195 (r=-0.221, P: 0.04 and r=-0.311, P: 0.005, respectively). Conclusion: Let-7d and miR-195 have been suggested to be involved in BC through modulation of the ARGs including BCL2 and CASP3. The qRT-PCR and IHC studies demonstrated that decreased expression of let-7d and miR-195 prohibits apoptosis via downregulating CASP3 and increasing BCL2 expressions promoting BC progression. These results also hypothesize that let-7d and miR-195 along with apoptotic biomarkers (BCL2 and CASP3) can be used in the future to introduce novel, non-invasive molecular biomarkers for BC into clinical practice.
Adoption, acceptability and sustained use of digital interventions to promote physical activity among inactive adults: a mixed-method study

Introduction Despite the positive effects of physical activity (PA) to prevent lifestyle diseases and improve health and well-being, only one-third of Norwegian adults meet the minimum recommendations on PA. Digital interventions to promote PA in inactive adults may improve health and well-being by being available, personalized and adequate. Knowledge on users’ adoption, acceptability and sustainability of digital interventions to promote PA is still limited. Objective To investigate the adoption, acceptability and sustained use of three digital interventions for promoting PA among inactive adults. Design A randomized control trial (ONWARDS) with 183 participants assigned to 3 groups and followed up for 18 months. All participants received a wearable activity tracker with the personalized metric Personal Activity Intelligence (PAI) on a mobile app, two groups received additional access to online training and one group had also access to online social support. Methods A mixed-methods approach was used to address the study objective. Acceptability was evaluated through the System Usability Scale (SUS) (n = 134) at 6 months. Adoption and sustained use were evaluated through a set of questions administered at 12 months (n = 109). Individual interviews were performed at 6 months with a sample of participants (n = 18). Quantitative data were analyzed with descriptive statistics, whereas qualitative data were analyzed using the Framework approach. Results PAI was the most successful intervention, with satisfactory usability and positive effects on motivation and behavior change, contributing to high adoption and sustained use. Online social support had a high acceptability and sustained use, but the intervention was not perceived as motivational to increase PA. Online training had low adoption, usability and sustained use. The qualitative interviews identified five main themes: (1) overall approach to physical activity, (2) motivation, (3) barriers to perform PA, (4) effects of PA, and (5) usability and acceptability of the digital interventions. Conclusion Personalized digital interventions integrating behavior change techniques such as individual feedback and goal setting are more likely to increase acceptability, adoption and sustained use. Future studies should investigate which digital interventions or combinations of different interventions are more successful in promoting PA among inactive adults according to the characteristics and preferences of the users. Trial registration Clinical trial registered at ClinicalTrials.gov: NCT04526444.
Nonverbal Communication Analysis in Animated Series

The Bilu Mela animated film is a film produced by the State of Indonesia without using sound from human dubbing, especially in dialogue. The audience is required to understand the storyline through the body movements of the Bilu Mela film characters. In the Bilu Mela episode non water ghost animation, it tells about two blue (Bilu) and red (Mela) fish sleeping in their respective aquariums. Then the blue one woke up because he heard the sound of a chest moving from outside the aquarium. Then a genie appeared who came out of the magic teapot and cursed the blue one to become long and the mela to become fat. This genie will lose his body if doused with water, so the mela watered the genie's left hand and the genie's left hand disappeared. When Bilu wanted to wash the genie's body parts, the genie apologized to Bilu not to be doused with water, Bilu agrees with the condition that the genie changes their (Bilu & Mela) bodies back to their original shape. the researcher uses the theory of non-verbal communication which can also be interpreted as the creation and exchange of messages without using words, this communication uses body movements, gestures, intonation (pitch-lowness), eye contact, facial expressions, proximity and touch - feel
MolOptimizer: A Molecular Optimization Toolkit for Fragment-Based Drug Design

MolOptimizer is a user-friendly computational toolkit designed to streamline the hit-to-lead optimization process in drug discovery. MolOptimizer extracts features and trains machine learning models using a user-provided, labeled, and small-molecule dataset to accurately predict the binding values of new small molecules that share similar scaffolds with the target in focus. Hosted on the Azure web-based server, MolOptimizer emerges as a vital resource, accelerating the discovery and development of novel drug candidates with improved binding properties.
A Short Review on Machine Learning in Space Science and Exploration

Machine learning is revolutionizing space exploration by tackling massive datasets, empowering astronauts, and driving scientific breakthroughs. From Deep Space 1's autonomous navigation to the James Webb Space Telescope's AI-assisted exoplanet discovery, Machine learning is transforming the present and shaping the future. With missions like NASA's Parker Solar Probe and the development of AI-powered monitoring systems and astro robots, the possibilities for unravelling the cosmos and democratizing space exploration are limitless. The future of space exploration lies in harnessing the power of ML to unlock the universe's secrets and make them accessible to all.