Miracle-dz/UniFAR-FTUs huggingface dataset:
Adherence and Blocking of Candida Albicans to Cultured Vaginal Epithelial Cells: Treatments to Decrease Adherence

Background. Pathogenesis of mucosal microorganisms depends on adherence to the tissues they colonize and infect. For Candida albicans, cell surface hydrophobicity may play a significant role in tissue binding ability. Methods. A continuous cell line of vaginal epithelial cells (VEC) was grown in keratinocyte serum-free medium (KSFM) with supplements and harvested by trypsinization. VEC were combined with yeast cells to evaluate adherence and inhibition of adherence. In this experimental setup, yeast stained with fluorescein isothiocyanate were allowed to attach to VEC and the resulting fluorescent VEC were detected by flow cytometry. Results. VEC were cultured and examined daily after plating and showed morphology similar to basal epithelial cells. Culture media supplemented with estradiol showed increased VEC proliferation initially (first 24 h) but cell morphology was not altered. Fluorescinated Candida cells bound effectively to the cultured VEC. Using fresh cells exposed to various preparations of K-Y, we showed that all formulations of the product reduced Candida binding to VEC by 25% to 50%. While VEC were generally harvested for use in experiments when they were near confluent growth, we allowed some cultures to grow beyond that point and discovered that cells allowed to become overgrown or stressed appeared to bind yeast cells more effectively. Conclusion. Flow cytometry is a useful method for evaluating binding of stained yeast cells to cultured VEC and has demonstrated that commercially available products have the ability to interfere with the process of yeast adherence to epithelial cells.
Integrating nature and nurture: Implications of person–environment correlations and interactions for developmental psychopathology

The developmental interplay between nature and nurture is discussed, with particular reference to implications for research in developmental psychopathology. The general principles include individual differences in reactivity to the environment, two-way interplay between intraindividual biology and environmental influences, and the need to consider broader social contextual features. Individuals actively process their experiences; they also act on their environment to shape and select their experiences, and individual characteristics change over time. Key findings on genetic effects include their ubiquitous influence, the multifactorial origin of most psychopathology, the involvement of several genes in most mental disorders, some genetic effects operate through dimensional risk features rather than directly on disorder, some genetic effects are dependent on gene–environment correlations and interactions, and genetic effects increase with age. Key findings on environmental effects include their ubiquitous influence, the genetic mediation of some supposed environmental effects, the importance of passive gene–environment correlations, the paucity of evidence regarding environmental effects on lifetime liability to psychopathology, the lack of understanding of environmental effects on the organism, and the importance of nonshared environmental effects. Research strategies to investigate environmental risk mediation include the range of genetically sensitive designs, migration studies, secular trend investigations, studies of nonfamilial environments, and examination of intraindividual change in relation to measured environmental alterations. Proximal processes involved in person–environment interplay are discussed in relation to person–environment interactions and evocative and active person–environment correlations.
An 8-Point IDCT computing resource implemented on a TriMedia/CPU64 reconfigurable functional unit

This paper presents the implementation of an 8-point Inverse Discrete Cosine Transform (IDCT) comput- ing resource on a TriMedia/CPU64 FPGA-based Reconfig- urable Functional Unit (RFU). TriMedia/CPU64 is a 64-bit 5 issue-slot VLIW processor launching a long instruction ev- ery clock cycle. The RFU consists mainly of an FPGA core, and is embedded into the TriMedia as any other hardwired functional unit, i.e., it receives instructions from the instruc- tion decoder, reads its input arguments from and writes the computed values back to the register file. To reduce the computational complexity of IDCT, we used a modified ver- sion of the Loeffler algorithm which requires 14 multipli- cations. Since each multiplicand is a 16-bit signed number represented in 2's complement notation, while each multi- plier is a positive constant of 15 bits or less, we employed a "multiplication-by-constant" scheme which was optimized against the multiplier. To increase the throughput of the IDCT computing resource, we propose a pipeline implemen- tation. When mapped on an ACEX EP1K100 FPGA-based RFU, our 8-point IDCT computing resource exhibits a la- tency of 16 TriMedia cycles, a recovery of 2 cycles, and oc- cupies 42% of the logic cells of the device.
Phylogenetic relationship of Salmonella enterica strains in Tehran, Iran, using 16S rRNA and gyrB gene sequences.

INTRODUCTION We assessed whether 16S rDNA and gyrB gene sequences, alone or combined, were suitable for determining the phylogenetic relationship among Salmonella enterica strains isolated from Tehran, Iran. Patients over five years of age enrolled in an acute diarrheal surveillance project in Tehran province between May 2004 and October 2006 were selected as our study group. METHODOLOGY 16S ribosomal DNA (rDNA) and gyrB genes from 40 Salmonella isolates obtained from patients with acute diarrhea were sequenced and the data was used to generate phylogenetic trees that facilitated isolate comparison. RESULTS Salmonella strains clustered into five to seven phylogenetic groups, dependent on analysis of 16S rDNA (1546 bp), gyrB (1256 bp) or a combination of the two genes. By 16S rDNA sequence analysis, only strains of Salmonella enterica serovar Typhi ( S. Typhi) clustered exclusively together. gyrB sequences permitted clustering of all the S. Typhi and S. Paratyphi A isolates, and clustering of S. Enteritidis into two separate but exclusive groups. Concatenation of the two data sets did not significantly improve the resolution of the strains compared to the gyrB gene. None of the analyses completely resolved S. enterica Paratyphi B and C into mutually exclusive groups. CONCLUSION Sequencing of gyrB represents a potentially useful tool for determining the phylogenetic relationship of S. enterica strains in Tehran, Iran. Genetic analysis of the 16S rRNA gene alone or in combination with gyrB did not increase the resolution between serotypes of S. enterica. We speculate that inclusion of additional genetic markers would improve the sensitivity of the analysis.
Climate Change and Society: The Chimera of Behaviour Change Technologies

Our dependence on energy from fossil fuels is causing potentially disastrous global warming and posing fundamental questions about the commensurability of consumer capitalism and a sustainable society. UK and Scottish governments have taken a lead in climate change legislation intended to avoid worst-case scenarios through low carbon transition. There are, however, considerable uncertainties about whether individualized, market-driven, materialistic societies can manage such radical transformations. Policies to cut household emissions focus on behaviour change through social marketing and incremental modifications to consumption. This technocratic model produces very little societal change, and seems likely to be self-defeating. The framing of the problem as one of behavioural adjustments to individual self-interest obscures alternative understandings of society as a collective accomplishment. Through simultaneous ‘knowing and not knowing’ about unsustainable consumerism, a behavioural model allows governing to proceed, while marginalizing awkward questions about the contradictions between economic growth and low carbon transitions.
Hybrid impedance control of a 3-DOF robotic arm used for rehabilitation treatment

A 3-DOF planar robotic arm is designed and used as a rehabilitation treatment tool for the survivor after suffering a stroke, aiming to assist them to recover upper-limb function. In order to strengthen the patient's arm, some forces are desired to be followed while the position trajectories should be tracked simultaneously, therefore hybrid impedance control (HIC) is proposed. Due to the redundancy for the planar motion, besides the main task, the secondary task is combined into the control scheme and the constraints of joint limits avoidance(JLA) are considered. Some typical trajectories are simulated for rehabilitation motion and a couple of simulations are made to study both the impedance control and hybrid impedance control strategies. The simulation results are compared and discussed and the tracking effectiveness of the proposed method is confirmed.
Involvement of 3p Deletions in Sporadic and Hereditary Forms of Renal Cell Carcinoma

Deletions of the short arm of chromosome 3 and associated allele losses have been reported in the majority of sporadic renal cell carcinomas (RCC). On the basis of the combined cytogenetic and molecular data, it is reasonable to assume that a putative RCC locus, which contributes to tumor development by its loss, is located telomerically of the D3F15S2 site. Using H3E4, a D3F1SS2‐specific probe, we have isolated a cDNA clone (c1.4–2), and a sequence comparison revealed that the cDNA clone corresponds to the human acyl‐peptide hydrolase gene. The gene is fairly universally expressed, but in RCC biopsies its expression is severely reduced, compared to the normal kidney. Cl.4–2 was used for in situ hybridization on metaphase chromosomes prepared from an Epstein‐Barr virus (EBV) transformed lymphoblastoid cell line, derived from a t(3;8) (p14.2;q24.1) carrying member of the RCC family described by Cohen et al. in 1979 (N Engl J Med: 301:592–595). Carriers of this translocation regularly develop RCC by middle age. We now report that D3F15S2 is localized on the telomeric side of the constitutional breakpoint, in 3p21. The region of 3p affected by this familial translocation is thus not identical with the region of 3p most frequently deleted in sporadic RCC.
Circulating MicroRNA-208b and MicroRNA-499 Reflect Myocardial Damage in Cardiovascular Disease

Background—Small RNA molecules, called microRNAs, freely circulate in human plasma and correlate with varying pathologies. In this study, we explored their diagnostic potential in a selection of prevalent cardiovascular disorders. Methods and Results—MicroRNAs were isolated from plasmas from well-characterized patients with varying degrees of cardiac damage: (1) acute myocardial infarction, (2) viral myocarditis, (3) diastolic dysfunction, and (4) acute heart failure. Plasma levels of selected microRNAs, including heart-associated (miR-1, -133a, -208b, and -499), fibrosis-associated (miR-21 and miR-29b), and leukocyte-associated (miR-146, -155, and -223) candidates, were subsequently assessed using real-time polymerase chain reaction. Strikingly, in plasma from acute myocardial infarction patients, cardiac myocyte–associated miR-208b and -499 were highly elevated, 1600-fold (P<0.005) and 100-fold (P<0.0005), respectively, as compared with control subjects. Receiver operating characteristic curve analysis revealed an area under the curve of 0.94 (P<10−10) for miR-208b and 0.92 (P<10−9) for miR-499. Both microRNAs correlated with plasma troponin T, indicating release of microRNAs from injured cardiomyocytes. In viral myocarditis, we observed a milder but significant elevation of these microRNAs, 30-fold and 6-fold, respectively. Plasma levels of leukocyte-expressed microRNAs were not significantly increased in acute myocardial infarction or viral myocarditis patients, despite elevated white blood cell counts. In patients with acute heart failure, only miR-499 was significantly elevated (2-fold), whereas no significant changes in microRNAs studied could be observed in diastolic dysfunction. Remarkably, plasma microRNA levels were not affected by a wide range of clinical confounders, including age, sex, body mass index, kidney function, systolic blood pressure, and white blood cell count. Conclusions—Cardiac damage initiates the detectable release of cardiomyocyte-specific microRNAs-208b and -499 into the circulation.
Influence of two barrier membranes on staged guided bone regeneration and osseointegration of titanium implants in dogs: part 1. Augmentation using bone graft substitutes and autogenous bone.

OBJECTIVES To assess the influence of two barrier membranes and two bone graft substitutes mixed with autogenous bone (AB) on staged guided bone regeneration and osseointegration of titanium implants in dogs. MATERIALS AND METHODS Four saddle-type defects each were prepared in the upper jaw of six fox hounds and randomly filled with a natural bone mineral (NBM)+AB and a biphasic calcium phosphate (SBC)+AB and allocated to either an in situ gelling polyethylene glycol (PEG) or a collagen membrane (CM). At 8 weeks, modSLA titanium implants were inserted and left to heal in a submerged position. At 8+2 weeks, dissected blocks were processed for histomorphometrical analysis (e.g., treated area [TA], bone-to-implant contact [BIC]). RESULTS The mean TA values (mm(2) ) and BIC values (%) tended to be higher in the PEG groups(TA: NBM+AB [10.4 ± 2.5]; SBC+AB [10.4 ± 5.8]/BIC: NBM+AB [86.4 ± 20.1]; SBC+AB [80.1 ± 21.5]) when compared with the corresponding CM groups (TA: NBM+AB [9.7 ± 4.8]; SBC+AB [7.8 ± 4.3]/BIC: NBM+AB [71.3 ± 20.8]; SBC+AB [72.4 ± 20.3]). A significant difference was observed for the mean TA values in the SBC+AB groups. CONCLUSION It was concluded that all augmentation procedures investigated supported bone regeneration and staged osseointegration of modSLA titanium implants. However, the application of PEG may be associated with increased TA values.
Mindfulness-based Relapse Prevention for Substance Use Disorders: A Systematic Review and Meta-analysis

Objectives: Substance use disorder (SUD) is a prevalent health issue with serious personal and societal consequences. This review aims to estimate the effects and safety of Mindfulness-based Relapse Prevention (MBRP) for SUDs. Methods: We searched electronic databases for randomized controlled trials evaluating MBRP for adult patients diagnosed with SUDs. Two reviewers independently assessed citations, extracted trial data, and assessed risks of bias. We conducted random-effects meta-analyses and assessed quality of the body of evidence (QoE) using the Grading of Recommendations Assessment, Development, and Evaluation approach. Results: We identified 9 randomized controlled trials comprising 901 participants. We did not detect statistically significant differences between MBRP and comparators on relapse (odds ratio [OR] 0.72, 95% confidence interval [CI] 0.46–1.13, low QoE), frequency of use (standardized mean difference [SMD] 0.02, 95% CI −0.40 to 0.44, low QoE), treatment dropout (OR 0.81, 95% CI 0.40 to 1.62, very low QoE), depressive symptoms (SMD −0.09, 95% CI −0.39 to 0.21, low QoE), anxiety symptoms (SMD −0.32, 95% CI −1.16 to 0.52, very low QoE), and mindfulness (SMD −0.28, 95% CI −0.72 to 0.16, very low QoE). We identified significant differences in favor of MBRP on withdrawal/craving symptoms (SMD −0.13, 95% CI −0.19 to −0.08, I2 = 0%, low QoE) and negative consequences of substance use (SMD −0.23, 95% CI −0.39 to −0.07, I2 = 0%, low QoE). We found negligible evidence of adverse events. Conclusions: We have limited confidence in estimates suggesting MBRP yields small effects on withdrawal/craving and negative consequences versus comparator interventions. We did not detect differences for any other outcome. Future trials should aim to minimize participant attrition to improve confidence in effect estimates.
Melanin-concentrating hormone 1 receptor-deficient mice are lean, hyperactive, and hyperphagic and have altered metabolism

Melanin-concentrating hormone (MCH) is a cyclic 19-aa hypothalamic neuropeptide derived from a larger prohormone precursor of MCH (Pmch), which also encodes neuropeptide EI (NEI) and neuropeptide GE (NGE). Pmch-deficient (Pmch−/−) mice are lean, hypophagic, and have an increased metabolic rate. Transgenic mice overexpressing Pmch are hyperphagic and develop mild obesity. Consequently, MCH has been implicated in the regulation of energy homeostasis. The MCH 1 receptor (MCH1R) is one of two recently identified G protein-coupled receptors believed to be responsible for the actions of MCH. We evaluated the physiological role of MCH1R by generating MCH1R-deficient (Mch1r−/−) mice. Mch1r−/− mice have normal body weights, yet are lean and have reduced fat mass. Surprisingly, Mch1r−/− mice are hyperphagic when maintained on regular chow, and their leanness is a consequence of hyperactivity and altered metabolism. Consistent with the hyperactivity, Mch1r−/− mice are less susceptible to diet-induced obesity. Importantly, chronic central infusions of MCH induce hyperphagia and mild obesity in wild-type mice, but not in Mch1r−/− mice. We conclude that MCH1R is a physiologically relevant MCH receptor in mice that plays a role in energy homeostasis through multiple actions on locomotor activity, metabolism, appetite, and neuroendocrine function.
Rhythm, Timing and the Timing of Rhythm

Abstract This article reviews the evidence for rhythmic categorization that has emerged on the basis of rhythm metrics, and argues that the metrics are unreliable predictors of rhythm which provide no more than a crude measure of timing. It is further argued that timing is distinct from rhythm and that equating them has led to circularity and a psychologically questionable conceptualization of rhythm in speech. It is thus proposed that research on rhythm be based on the same principles for all languages, something that does not apply to the widely accepted division of languages into stress- and syllable-timed. The hypothesis is advanced that these universal principles are grouping and prominence and evidence to support it is provided.
In Search of Dominance: The Case of the Missing Dimension

Some previous researchers have found that affect can be described in terms of two dimensions (pleasure and arousal), while others have noted three dimensions are needed (pleasure, arousal, and dominance). The competing claims were tested by creating stimuli with factors previously demonstrated to elicit responses of arousal or dominance, asking respondents to rate the stimuli, and contrasting correlations between ratings and the stimulus factors. Under the two-dimensional theory, the planned contrasts should be zero, while under the three-dimensional theory, the planned contrasts should be nonzero. Results supported the three-dimensional model.
Use of supplemented Stainer-Scholte broth for the isolation of Bordetella pertussis from clinical material

The use of Stainer-Scholte broth supplemented with (2,6-O-dimethyl)beta-cyclodextrin (heptakis) for the isolation of Bordetella pertussis from clinical specimens was evaluated with 3,632 nasal swabs from children and adults with suspected whooping cough or from their family contacts. The liquid enrichment medium was subcultured on charcoal agar with 10% defibrinated horse blood. Charcoal agar and soft charcoal agar served as the standard procedure to detect B. pertussis. We isolated 772 strains of B. pertussis (21%). Charcoal agar alone detected 87% of all strains (n = 668), soft charcoal agar grew 78% (n = 602), and 637 strains (83%) were isolated when Stainer-Scholte broth with heptakis was used. We detected 590 isolates with all three media. Whereas 65 strains grew only on charcoal agar, 27 strains were detected by soft charcoal agar. Supplemented Stainer-Scholte broth allowed the isolation of an additional 77 strains which did not primarily grow on charcoal media (P less than 0.05). Our data indicate that Stainer-Scholte medium supplemented with heptakis can be effectively used as an enrichment medium for detection of B. pertussis in clinical specimens.
Finding Optimal Neural Network Architecture Using Genetic Algorithms

This work deals with methods for finding optimal neural network architectures to learn particular problems. A genetic algorithm is used to discover suitable domain specific architectures; this evolutionary algorithm applies direct codification and uses the error from the trained network as a performance measure to guide the evolution. The network training is accomplished by the back-propagation algorithm; techniques such as training repetition, early stopping and complex regulation are employed to improve the evolutionary process results. The evaluation criteria are based on learning skills and classification accuracy of generated architectures
A Longitudinal Study of Depressive Symptomology and Self-Concept in Adolescents

The purpose of the study was to investigate the trajectories of depressive symptomology and self-concept in adolescents between the ages of 13 and 17 and to determine whether primary school teacher ratings of adaptive and maladaptive behavior predict self-reported depressive symptoms and self-concept in adolescence. This study is part of an ongoing longitudinal study of a school-based sample of students representing a continuum of risk for developing emotional and behavioral disorders (EBD; N = 212). Results indicated a strong relationship between depressive symptoms and self-concept for both initial status and growth over time (i.e., depressive symptoms decreased, whereas self-concept improved). No effect was found for gender on initial status or growth for either construct. However, for self-concept, the growth trajectory indicated a significant effect for risk classification (not at risk, at risk no special education placement, at risk special education placement). Compared with the other classification groups, adolescents in special education at risk for EBD showed a significant decrease in self-concept after age 15, a markedly different trajectory from their peers. Additionally, high internalizing behavior was associated with more depressive symptoms and lower self-concept. Finally, early teacher ratings of adaptive behavior were predictive of both initial status and growth for depressive symptoms, but only initial status for self-concept. Implications for depression prevention and intervention among children and adolescents are discussed.
Markov Process Modeling of A System Under WIPLOAD Control

This paper analyzes a proposed release control methodology, WIPLOAD Control (WIPLCtrl), using a transfer line case modeled by Markov process modeling methodology. The performance of WIPLCtrl is compared with that of CONWIP under 13 system configurations in terms of throughput, average inventory level, as well as average cycle time. As a supplement to the analytical model, a simulation model of the transfer line is used to observe the performance of the release control methodologies on the standard deviation of cycle time. From the analysis, we identify the system configurations in which the advantages of WIPLCtrl could be observed.
IP Multimedia Communications

Systems-on-Chip architecture integrates several heterogeneous components on a single chip. A key challenge is to design the communication between the different entities of a SoC in order to minimize the communication overhead. Network-on-chip (NoC) is a new approach for communication infrastructure of Systems-on-Chip (SoC) design, which provides network based solution for on-chip communication. Networks on Chip can be designed in different ways, according to the network architecture and protocol choice. It is important to balance the communication needs across the different links to avoid congestion and hot spots especially for