Miracle-dz/UniFAR-FTUs huggingface dataset:
International Journal of Current Research in

WRKY proteins are a class of plant-specific transcription factors involved in stress response signaling pathways. In this work, a novel WRKY transcription factor gene, named after GbWRKY31, was isolated by RT-PCR method from the Ginkgo biloba. The full-length cDNA of GbWRKY31 was 1738 bp and contained a 1281bp open reading frame (ORF) encoding 427 amino acids. The estimated isoelectric point (pI) and molecular weight of the putative GbWRKY31 protein were 9.10 and 47.05 kDa, respectively. Homology analysis indicated that the deduced GbWRKY31 protein was highly homologous to other WRKY Proteins from different species and had a typical WRKY conserved domain database. Phylogenetic analysis showed that GbWRKY31 belongs to the WRKY IIb group. The results suggest that GbWRKY31 belongs to WRKY families and may be involved in the modulation of senescence and defense in G. biloba. Accepted: 01 July 2016 Available Online: 06 July 2016
International Journal of Current Research in

Date of Publication: 06 September 2019 This study is aimed at a better knowledge of the genetic diversity of H. cannabinus, a leafy vegetable which is very consumed in Burkina Faso. For this, a molecular characterization using SSRs microsatellite markers of 70 accessions collected in the three climatic zones of the country was carried out. The analysis shows significant genetic diversity among accessions, structured into three genetic groups. Indeed, 17 of the 20 markers tested were polymorphic. Expected unbiased heterozygosity ranged from 0.131 for marker MJM566 to 0.435 for marker MJM606 with an average of 0.309 for all primers. The Shannon diversity index (I) ranges from 1.552 for the primer MJM566 to 5.008 for the primer MJM606 with an average of 3.73 for all the primers tested. The organization of this genetic diversity is strongly influenced by the genetic group factor with a value of the differentiation index Fst of 0.324. Keywords
International Journal of Current Research in

Date of Publication: 06 July 2018 OsiEZ1 is a member of evolutionary conserved Polycomb Repressive Complex 2 (PRC2) group of proteins which participate in stable maintenance of repressive states of genes on which they act upon. It belongs to Enhancer of Zeste (E(Z)) class, the proteins which carry the SET domain having histone methyltransferase activity. The PRC2 complex components and other SET-domain containing proteins act in a multitude of developmental processes in plants. The prominent aspects of plant life controlled by them include vegetative to floral transition, maintenance of meristem identity, vernalization; and gametophyte and seed development. To ascertain the role of OsiEZ1 gene in plant development, antisense transgenics were constructed in Oryza sativa L. subspecies indica (var. Pusa Basmati 1) to partially suppress the functions of the endogenous gene. Some significant phenotypic changes were observed among a fraction of the R1 and R2 generation of OsiEZ1 antisense transgenic plants, with respect to the wild-type plants. The phenotypic differences observed were curling of leaves and multiple shoot formation at young seedling stages. Some of the transgenic plants (both in R1 and R2 generations) exhibited lethal phenotypes with profuse root growth, multiple shoot formation, dwarfism and leaf-senescence progressing from the tip to the base of the plant. Our preliminary studies definitely indicate that OsiEZ1 plays some important role in controlling early development in Pusa Basmati 1 rice plants. K e y w o r d s
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Cluster bean, Cyamopsis tetragonoloba L. is one of the most important and potential vegetable cum industrial crop grown for its tender pods as vegetable and for endospermic gum (30-35%). The present study was conducted in a net house using pot experiments on seven different cluster bean varietal plants namely HG365, JJ1, JG2, GL1031, REC1025, RGC963, and RGC936. In these guar plants the physical, biochemical and enzymatic parameters were studied in correlation to the guar gum content and Aphis craccivora K. infestation. The physical parameters like germination percentage, seedling vigour and vigour index were superior in RGC963, HG365, JJ1, REC1025 plant varieties resulting in less aphid infestation. In addition, the gum content, total soluble sugars and total protein content were high in guar varieties of RGC963, HG365, JJ1, REC1025 followed by JG2, GL1031, and RGC936 along with a reduced content of amino acids. Whereas, enhanced levels of primary metabolites and antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were observed in the aphid infested cluster bean plants in comparison to normal, uninfested plants in all the varieties. This study reveals the possible role of enhanced plant biochemicals like carbohydrates and phenols towards higher yield of guar gum and lower aphid infestation respectively. In future the present research can lead us to the development of cluster bean varietal plants with higher gum yield potential for desired resistance towards biotic stresses. Accepted: 01 April 2016 Available Online: 06 April 2016
International Journal of Current Research in

Propylene glycol (PG) and calcium propionate (CP) in saline solutions were drenched twice weekly to 18 Egyptian water-buffalo heifers at the last 8 weeks of gestation, besides a control group. The overall means of both treatments (particularly CP) significantly increased (P≤0.05) the feed intake of all ingredients, live body weight, reproductive performance; teat diameter, size, side area, area and length; calve birth weight, as well as daily and total milk production compared with the control. Both treatments significantly (P≤0.05) reduced blood urea nitrogen, β-hydroxybutyrate and non-essential fatty acids' values; but increased blood insulin, T3, and T4, concentrations and T3/T4 ratio. The CP treatment elevated (P≤0.05) also blood glucose level and milk fat and protein but reduced milk lactose %. Sampling time affected significantly (P≤0.05) daily feed intake, milk fat and lactose % as well as blood Na, K, Ca, P, T4 and insulin values, in addition to body condition score, live body weight, and udder form. Accepted: 20 November 2017 Available Online: 06 December 2017
Determination of Saponins in Leaf of Panax Ginseng C. A. Mey. by High Performance Liquid Chromatography

A high performance liquid chromatography(HPLC) with UV detection was established for simultaneous determination of saponins in the leaf of Panax ginseng C. A. Mey. Nine ginsenosides(Rb1,Rb2,Rb3,Rc,Rd,F1,F2,F3,F5) and notoginsenoside Fe(NFe) were studied. Among the saponins,the ginsenosides F1,F2,F3,F5 and NFe were determined by HPLC-UV method for the first time. The determination of the ginsenosides via the HPLC-UV method was performed on a reversed-phase C18 column with gradient elution in 40 min. The linearity,precision,accuracy,and detection limit for determining the saponins were studied and the samples from different areas in China were analyzed. The HPLC-ESI-MS was used to identify the saponins. The results indicate that the HPLC-UV pro-vided a good accuracy,reproducibility and sensitivity for the determination of the ten saponins.
International Journal of Current Research in

G-protein coupled receptors (GPCRs) are the largest family of membrane proteins, as key components of signal transduction pathways. Despite the current knowledge, GPCRs in plants are not well characterized if compared with animals and are still a matter of controversy. The availability of plant genomes allows the prediction and characterization of diverse GPCRs, as for several species the repertoire of GPCRs remains unclear. As a contribution to this problem, we present the in silico analyses of putative GPCRs candidates from Arabidopsis thaliana, Capsicum annuum, Glycine max, Manihot esculenta, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera and Zea mays proteomes. From a total of 997,435 proteins collected, the sequences were selected by length, reducing the initial collection to 487,012. GPCRpipe program predicted 60 GPCRs candidates, ranging from none (Capsicum annuum) to 12 candidates (Populus trichocarpa) (median = 3 ± 2.90). The Neighbor joining dendrogram of the 60 predicted GPCR candidates grouped the proteins in three clades. These findings may indicate that GPCR in plants are involved in drought stress responses. Accepted: 01 November 2016 Available Online: 06 November 2016
International Journal of Current Research in

The nutrition care process (NCP) is a four-step approach to nutrition problem solving and care that is designed to guide and illuminate the work of the professionals. NCP model provides a consistent structure and framework for food and nutrition professionals to use while delivering nutrition care. A standardized language in form of International Dietetic and Nutrition Terminology (IDNT) has been in place to communicate, document and evaluate the efficacy of nutrition care and may translate into improved clinical outcomes. Implementation of the NCP helps to generate comparative data and information relating to the impact of nutrition interventions to resolve nutrition problems. Malnutrition is one of the important features of chronic kidney disease (CKD) and it occurs in varying degrees depending on stage of CKD. Various studies have supported the implementation of a systematic approach to patient care in line with NCP and have shown improvement in dietary intake and nutritional status among CKD patients. Providing individualised nutritional counselling improves many components of quality of life prior to dialysis treatment. The overall goal of implementing NCP among CKD patients is to match dietary intake with existing kidney function or renal replacement therapy while preventing nutritional deficiencies. Literature published between January 1985 and January 2016 in peer reviewed scientific journals were considered. Only full text articles in English were included. This review article attempts to highlight the important features of Nutrition Care Process (NCP) and International Dietetics and Nutrition Terminology (IDNT) and its potential application in chronic kidney disease (CKD). Accepted: 27 May 2016 Available Online: 06 June 2016
Correlation between the atmospheric visibility and aerosol fine particle concentrations in Guangzhou and Beijing.

In order to study the effects of relative humidity(RH) and aerosol fine particles(PM2.5 and PM10) on visibility,visibility,RH,PM2.5 and PM10 during 11th December,2008~27th August,2009 in Guangzhou,and 14th July~17th September,2008 in Beijing were measured,respectively.PM2.5 and RH were the main factors on effecting visibility.When the RH was lower than 70% and between 80% and 90%,the effects of PM2.5 on visibility in Guangzhou and Beijing were different.However,a good correlation between visibility and PM2.5 was found both in Guangzhou and Beijing when RH was between 70% and 80%. A division was found in 0.05mg/m3 from the fitting curve.The visibility did not vary obviously with the PM2.5 concentration reduction when the concentration of PM2.5 was higher than 0.05mg/m3.However,a rapid improving in visibility was observed with decreasing of the PM2.5 concentration when the concentration of PM2.5 was lower than 0.05mg/m3.Therefore,the reduction on PM2.5 did not show obvious improving in visibility at the early time of controlling PM2.5,whereas the visibility could be remarkably improved when the concentration of PM2.5 reduced to a certain value.
Adaptive Antenna Array for Low Signal-to-Noise Ratio Operation

The adaptive antenna array which operates in the low signal-to-noise ratio (SNR) is presented. In this scenario, the Minimum Mean Squares Error (MMSE) criterion adaptive algorithms cannot be used for the array weight calculation. If the angular location of the signal source is known, it is possible to use the linearly constrained adaptive algorithms. However, this location is often unknown or it is changed if the signal source or the array is moving. At the same time, if the informational signals are the pseudo-noise sequences, they can be pre-processed by the matched filters or by the correlators. In this case the informational signal at the devices output becomes above the noise (the SNR is increased) and the MMSE criterion algorithms can be used for the calculation of the adaptive array weights. The adaptive array based on this criterion is able to suppress the interference signals. Besides, it is also able to steer the beam in the direction of the informational signal source and to track the source. The computational procedures of the two Recursive Least Squares algorithms which process the matched filter output signals for the calculation of the adaptive array weights are considered. One of these algorithms uses the complex-valued arithmetic and other one uses the real-valued arithmetic. The simulation demonstrates that the real-valued arithmetic algorithm has about 3 dB better cancellation of the interference signals at the adaptive array output and about two times shorter transient response.
Two Birds with One Stone: A Novel Dithiomaleimide-Based GalNAc-siRNA Conjugate Enabling Good siRNA Delivery and Traceability

For the first time, a novel dithiomaleimides (DTM) based tetra-antennary GalNAc conjugate was developed, which enable both efficient siRNA delivery and good traceability, without incorporating extra fluorophores. This conjugate can be readily constructed by three click-type reactions, that is, amidations, thiol-dibromomaleimide addition and copper catalyzed azide–alkyne cycloaddition (CuAAC). And it also has comparable siRNA delivery efficiency, with a GalNAc L96 standard to mTTR target. Additionally, due to the internal DTMs, a highly fluorescent emission was observed, which benefited delivery tracking and reduced the cost and side effects of the extra addition of hydrophobic dye molecules. In all, the simple incorporation of DTMs to the GalNAc conjugate structure has potential in gene therapy and tracking applications.
Performance-Derived Behavior Vocabularies: Data-Driven Acquisition of Skills from Motion

Control for and interaction with humanoid robots is often restrictive due to limitations of the robot platform and the high dimensionality of controlling systems with many degrees of freedom. We focus on the problem of providing a "skill-level interface" for a humanoid robot. Such an interface serves as (i) a modular foundation for structuring task-oriented control, (ii) a parsimonious abstraction of motor-level control (e.g. PD-servo control), and (iii) a means for grounding interactions between humans and robots through common skill vocabularies. Our approach to constructing skill-level interfaces is two-fold. First, we propose a representation for a skill-level interface as a "behavior vocabulary," a repertoire of modular exemplar-based memory models expressing kinematic motion. A module in such a vocabulary encodes a flow field (or gradient field) in joint angle space that describes the "flow" of kinematic motion for a particular skill-level behavior, enabling prediction from a given kinematic configuration. Second, we propose a data-driven method for deriving behavior vocabularies from time-series data of human motion using spatio-temporal dimension reduction and clustering. Results from evaluating an implementation of our methodology are presented along with the application of derived behavior vocabularies as predictors towards on-line humanoid trajectory formation and off-line motion synthesis.
Carbon Sequestration and Storage of Citrus Orchard System in Three Gorges Reservoir Region of Chongqing

As a part of agricultural vegetation,orchard ecosystem plays an important role in the evaluation of carbon sink of terrestrial ecosystems in China. In order to observe the carbon stocks of citrus orchard system in the Three Gorges Reservoir region of Chongqing,the characteristics of carbon storage and distribution of citrus orchard system were studied by field investigation and on-the-spots survey of classified orchard system( young citrus orchard,forest citrus orchard and old citrus orchard). The result shows that biomass of each citrus plant organ was closely relative to the stem base diameter; this model can be used to predict the biomass of citrus plant and different organs. Citrus biomass with base diameter increases,but the biomass and carbon content of each organ was significant different. The citrus orchard biomass and carbon density is a dynamic increased process with citrus plant increasing age,in which the carbon density was about 157,214 and 222t·hm- 2in young citrus orchard,forest citrus orchard and old citrus orchard system,respectively. Therefore,the carbon storage was 36. 12million ton in the Three Gorges Reservoir region of Chongqing,in which about 88. 3 % in soil and 11. 7 % in citrus plant,and the economic value of that was more than 11. 49 billion Yuan. Thus,the great ecological construction application value was found in citrus orchard system in the Three Gorges Reservoir region of Chongqing.
Strain-specific and pooled genome sequences for populations

To contribute to our general understanding of the evolutionary forces that shape variation in genome sequences in nature, we have sequenced genomes from 50 isofemale lines and six pooled samples from populations of on three continents. Analysis of raw and Drosophila melanogaster reference-mapped reads indicates the quality of these genomic sequence data is very high. Comparison of the predicted and experimentally-determined infection status of these samples Wolbachia suggests that strain or sample swaps are unlikely to have occurred in the generation of these data. Genome sequences are freely available in the European Nucleotide Archive under accession ERP009059. Isofemale lines can be obtained from the Species Stock Center. Drosophila
Mycobacterium tuberculosis: Pathogenesis and therapeutic targets

Abstract Tuberculosis (TB) remains a significant public health concern in the 21st century, especially due to drug resistance, coinfection with diseases like immunodeficiency syndrome (AIDS) and coronavirus disease 2019, and the lengthy and costly treatment protocols. In this review, we summarize the pathogenesis of TB infection, therapeutic targets, and corresponding modulators, including first‐line medications, current clinical trial drugs and molecules in preclinical assessment. Understanding the mechanisms of Mycobacterium tuberculosis (Mtb) infection and important biological targets can lead to innovative treatments. While most antitubercular agents target pathogen‐related processes, host‐directed therapy (HDT) modalities addressing immune defense, survival mechanisms, and immunopathology also hold promise. Mtb’s adaptation to the human host involves manipulating host cellular mechanisms, and HDT aims to disrupt this manipulation to enhance treatment effectiveness. Our review provides valuable insights for future anti‐TB drug development efforts.
The Performance of RSS Based Visible Light Positioning Techniques under Different Uniformity Conditions

As visible light positioning combines illumination and indoor localization, the relationship between the uniformity index as a quality measurement of the illumination and the precision of the positioning technique is investigated. It is demonstrated that the classic multilateration approach leads to a degradation of the accuracy as the uniformity improves. When a Gaussian Process is deployed, the accuracy improves for better uniformity indices. Further normalization of the area size leads to a generic curve that can be used to estimate the required training set size when a Gaussian Process is used given the required accuracy and observation plane size.
Overpressure Prediction In The North-West Niger Delta, Using Porosity Data

Overpressure prediction in the North West of Niger Delta, using porosity data was carried out to safeguard hazards associated with drilling accident due to blowout. In the absence of seismic data to predict overpressure, porosity-dependent parameters and acoustic impedance could be used to predict the tops of overpressured zones in the area of study in the Niger Delta. Overpressure prediction is vital for safe and economic drilling. Composite logs were used to obtain the required data by digitizing the logs and deduction using the appropriate relationships. The findings from the study show that porosity decreases with depth, with overpressure zone detected at about 3500m depth due to porosity deviation from normal trend. Pressure gradient in the upper normal pressure of the field is determined to be 0.989 psi/ft, this implies that within the established normal pressure gradient of 0.71 - 1.1 psi/ft in the Niger Delta. Formation overpressure gradient is determined to be 1.40 psi/ft. The overpressure zone coincides within the high shale-to-sand ratio of Agbada under compacted Formation. The identification of the tops of overpressure zones in any formation penetrated by a borehole enhances the use of normal drilling techniques of the borehole. This also reduces the cost of drilling the entire well as the special drilling technique will be applied only in the overpressure zones. This finding can aid in the prevention of drilling accident and resource wastage in exploration activities.
Is Coffee and Tea a Threat or Ally to Cardiovascular Health?

Tea and coffee have become ingrained in our daily lives and have become the most widely consumed drinks after water. Their effects vary on an individual basis depending upon the amount of daily consumption, genetic polymorphisms, and the presence of comorbidities. Non-habitual individuals experience an initial, brief increase in blood pressure due to caffeine's vasoactive effects. Caffeine also appears to be protective against arrhythmias and heart failure. Along with having a generally cardioprotective profile, they have also demonstrated to have a favorable impact on insulin resistance and reduced risk of diabetes mellitus. Physicians often practice caution and advise patients with known cardiovascular diseases to refrain from drinking caffeine; however, studies have shown that drinking two to three cups a day has either no or some beneficial effects on both patients with or without cardiac disorders like arrhythmias. This article focuses on the effects of tea and coffee on the cardiovascular system as well as the potential mechanisms involved.