Miracle-dz/UniFAR-FTUs huggingface dataset:
Three-dimensional assessment of the asymptomatic and post-stroke shoulder: intra-rater test–retest reliability and within-subject repeatability of the palpation and digitization approach

Abstract Purpose: Altered three-dimensional (3D) joint kinematics can contribute to shoulder pathology, including post-stroke shoulder pain. Reliable assessment methods enable comparative studies between asymptomatic shoulders of healthy subjects and painful shoulders of post-stroke subjects, and could inform treatment planning for post-stroke shoulder pain. The study purpose was to establish intra-rater test–retest reliability and within-subject repeatability of a palpation/digitization protocol, which assesses 3D clavicular/scapular/humeral rotations, in asymptomatic and painful post-stroke shoulders. Methods: Repeated measurements of 3D clavicular/scapular/humeral joint/segment rotations were obtained using palpation/digitization in 32 asymptomatic and six painful post-stroke shoulders during four reaching postures (rest/flexion/abduction/external rotation). Intra-class correlation coefficients (ICCs), standard error of the measurement and 95% confidence intervals were calculated. Results: All ICC values indicated high to very high test–retest reliability (≥0.70), with lower reliability for scapular anterior/posterior tilt during external rotation in asymptomatic subjects, and scapular medial/lateral rotation, humeral horizontal abduction/adduction and axial rotation during abduction in post-stroke subjects. All standard error of measurement values demonstrated within-subject repeatability error ≤5° for all clavicular/scapular/humeral joint/segment rotations (asymptomatic ≤3.75°; post-stroke ≤5.0°), except for humeral axial rotation (asymptomatic ≤5°; post-stroke ≤15°). Conclusions: This noninvasive, clinically feasible palpation/digitization protocol was reliable and repeatable in asymptomatic shoulders, and in a smaller sample of painful post-stroke shoulders. Implications for Rehabilitation In the clinical setting, a reliable and repeatable noninvasive method for assessment of three-dimensional (3D) clavicular/scapular/humeral joint orientation and range of motion (ROM) is currently required. The established reliability and repeatability of this proposed palpation/digitization protocol will enable comparative 3D ROM studies between asymptomatic and post-stroke shoulders, which will further inform treatment planning. Intra-rater test–retest repeatability, which is measured by the standard error of the measure, indicates the range of error associated with a single test measure. Therefore, clinicians can use the standard error of the measure to determine the “true” differences between pre-treatment and post-treatment test scores.
Aedes Mosquitoes and Aedes-Borne Arboviruses in Africa: Current and Future Threats

The Zika crisis drew attention to the long-overlooked problem of arboviruses transmitted by Aedes mosquitoes in Africa. Yellow fever, dengue, chikungunya and Zika are poorly controlled in Africa and often go unrecognized. However, to combat these diseases, both in Africa and worldwide, it is crucial that this situation changes. Here, we review available data on the distribution of each disease in Africa, their Aedes vectors, transmission potential, and challenges and opportunities for Aedes control. Data on disease and vector ranges are sparse, and consequently maps of risk are uncertain. Issues such as genetic and ecological diversity, and opportunities for integration with malaria control, are primarily African; others such as ever-increasing urbanization, insecticide resistance and lack of evidence for most control-interventions reflect problems throughout the tropics. We identify key knowledge gaps and future research areas, and in particular, highlight the need to improve knowledge of the distributions of disease and major vectors, insecticide resistance, and to develop specific plans and capacity for arboviral disease surveillance, prevention and outbreak responses.
Adjusting modulus of elasticity of lumber for changes in temperature

This study evaluated the immediate effect of temperature on flexural modulus of elasticity (MOE) of green and dry nominal 2- by 4-inch (standard 38- by 89-mm) structural lumber at temperatures ranging from 150° to -15°F (66° to 26°C). For lumber at 12 percent moisture content (MC), a linear relationship was used to relate the increase in MOE to the decrease in temperature. For green lumber, MOE also increased with decreasing temperature. A segmented linear regression was developed to describe the change in MOE for temperatures from 150° to 0°F (66° to -18°C). The slope of this relationship was steeper below 32°F (0°C) than above this temperature. The actual MC of green lumber was not a factor above 0°F (-18°C). Below 0°F, the increase in MOE with decreasing temperature was a function of both actual green MC and temperature. We discuss factors that cause this behavior in green wood at low temperatures and the use of an empirical model for describing the MOE-temperature relationship as a function of MC for frozen green lumber. The model is believed to be independent of softwood species and lumber grade. Relative to MOE at 100°F (38°C), results of this research support the C t factors given in the National Design Specifications (1).
Morphological diversity and development of glia in Drosophila

Insect glia represents a conspicuous and diverse population of cells and plays a role in controlling neuronal progenitor proliferation, axonal growth, neuronal differentiation and maintenance, and neuronal function. Genetic studies in Drosophila have elucidated many aspects of glial structure, function, and development. Just as in vertebrates, it appears as if different classes of glial cells are specialized for different functions. On the basis of topology and cell shape, glial cells of the central nervous system fall into three classes (Fig. 1 A–C): (i) surface glia that extend sheath‐like processes to wrap around the entire brain; (ii) cortex glia (also called cell body‐associated glia) that encapsulate neuronal somata and neuroblasts which form the outer layer (cortex) of the central nervous system; (iii) neuropile glia that are located at the interface between the cortex and the neuropile, the central domain of the nervous system formed by the highly branched neuronal processes and their synaptic contacts. Surface glia is further subdivided into an outer, perineurial layer, and an inner, subperineurial layer. Likewise, neuropile glia comprises a class of cells that remain at the surface of the neuropile (ensheathing glia), and a second class that forms profuse lamellar processes around nerve fibers within the neuropile (astrocyte‐like or reticular glia). Glia also surrounds the peripheral nerves and sensory organs; here, one also recognizes perineurial and subperineurial glia, and a third type called “wrapping glia” that most likely corresponds to the ensheathing glia of the central nervous system. Much more experimental work is needed to determine how fundamental these differences between classes of glial cells are, or how and when during development they are specified. To aid in this work the following review will briefly summarize our knowledge of the classes of glial cells encountered in the Drosophila nervous system, and then survey their development from the embryo to adult. © 2011 Wiley‐Liss, Inc.
Oil palm shell as an agricultural solid waste in artificial lightweight aggregate concrete

The aim of this study was to produce a sustainable construction material by incorporating an agricultural solid waste, namely oil palm shell (OPS), in an artificial lightweight aggregate concrete. For this purpose, in a structural lightweight aggregate concrete made of expanded clay, the lightweight aggregate was substituted with OPS in 0, 25 and 50% by volume. Properties such as compressive strength under different curing conditions, as well as density, splitting tensile and flexural strengths, modulus of elasticity and drying shrinkage of expanded clay–OPS concretes were measured and discussed. The test results showed that partial substitution of expanded clay by OPS increased the density, compressive strength, specific strength (compressive strength to weight ratio), as well as splitting tensile and flexural strengths of lightweight concrete. However, it was observed that the modulus of elasticity decreased by about 4 and 13% in the 25 and 50% substitution levels, respectively. The expanded clay–OPS concretes showed greater drying shrinkage strain compared to expanded clay lightweight concrete. In addition, it was found that the sensitivity of compressive strength of concretes containing OPS to the lack of curing is due to high drying shrinkage and consequently micro-cracks formation in the interfacial transaction zone of the concretes.
Determinants of Quality of Life in Lung Cancer Patients

PURPOSE To examine the relationships of self-care, symptoms, and a variety of demographic factors to quality of life (QOL), and to identify determinants of QOL in lung cancer patients undergoing chemotherapy. DESIGN A cross-sectional, correlational study. METHODS 159 patients with lung cancer undergoing chemotherapy were recruited from three southern hospitals in Taiwan. Four instruments were used: the Quality of Life Questionnaire Core 30-item (QLQ-C30), M.D. Anderson Symptom Inventory (MDASI), Memorial Symptom Assessment Scale-Short Form (MSAS-SF), and Self-Care Behavior Scale (SCBS). FINDINGS Lung cancer patients rated lower scores of self-care behaviors on food choice and nutrition maintenance, regular exercise and sleep, and medical compliance. Being younger, having spouses as main caregivers, having food choice and nutrition maintenance, and getting regular exercise and sleep were associated with better QOL. Degree of interference with life, age, food choice and nutrition maintenance, and psychological symptoms were found to predict functional QOL and accounted for 43% of total variance. CONCLUSIONS The findings identified factors influencing QOL and provided evidence for designing an intervention to enhance QOL in lung cancer patients undergoing chemotherapy. CLINICAL RELEVANCE The findings may be useful for guiding intervention development for early detection and management of symptom interference with daily living, and place greater focus on patient self-care to promote food choice and nutrition maintenance, especially in older patients and those whose caregivers are not their spouses.
Damage identification of truss structures based on force method and free vibration analysis

The improved structural analysis is presented to determine the sites and extents of multiple damages of truss structures. The algorithm uses the mathematical programming of the finite element technique based on the force method and the free vibration analysis. The equilibrium matrix is generated through the finite element analysis, while the compatibility matrix is obtained directly using the force–displacement relations and the singular value decomposition technique. The governing equations with the element nodal forces as variables are presented by combining the equilibrium and compatibility matrices with the force–displacement relations. Next, the genetic algorithm is used to properly identify the sites and extents of multiple damage cases in truss structures. In order to verify the accuracy and superiority of the proposed damage detection technique, numerical solutions are presented for the planar and space truss models. The numerical results indicate that the combination of the force method and genetic algorithm can provide a reliable tool to accurately and efficiently identify the multiple damages of truss structures.
Enzymatic Evidence for a Complete Oxidative Pentose Phosphate Pathway in Chloroplasts and an Incomplete Pathway in the Cytosol of Spinach Leaves

The intracellular localization of transaldolase, transketolase, ribose-5-phosphate isomerase, and ribulose-5-phosphate epimerase was reexamined in spinach (Spinacia oleracea L.) leaves. We found highly predominant if not exclusive localization of these enzyme activities in chloroplasts isolated by isopyknic centrifugation in sucrose gradients. Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glucose phosphate isomerase, and triose phosphate isomerase activity was present in the chloroplast fraction but showed additional activity in the cytosol (supernatant) fraction attributable to the cytosol-specific isoforms known to exist for these enzymes. Anion-exchange chromatography of proteins of crude extracts on diethylaminoethyl-Fractogel revealed only a single enzyme each for transaldolase, transketolase, ribose-5-phosphate isomerase, and ribulose-5-phosphate epimerase. The data indicate that chloroplasts of spinach leaf cells possess the complete complement of enzymes of the oxidative pentose phosphate path-way (OPPP), whereas the cytosol contains only the first two reactions, contrary to the widely held view that plants generally possess a cytosolic OPPP capable of cyclic function. The chloroplast enzymes transketolase, ribose-5-phosphate isomerase, and ribulose-5-phosphate epimerase appear to be amphibolic for the Calvin cycle and OPPP.
Optical studies on phthalocyanines substituted with phenylazonaphthoxy groups

Abstract The synthesis and characterization of peripherally ({1-[(Z or E)-phenylazo]-2-naphthyl} oxy) tetra-substituted metal-free and metallo (Zn(II), Co(II) and Cu(II)) phthalocyanines are described in this study. Aggregation properties of these compounds were investigated in the concentration range of 20–1 μM in tetrahydrofuran. Spectroscopic and photophysical properties of these phthalocyanines have been investigated in tetrahydrofuran. Thin films of metallophthalocyanines were prepared by spin coating technique. Results show that transmittance values of the films change with respect to the metal ions in the visible and Q absorptions regions. Films were also annealed at 100, 200 and 300 °C and their optical properties were investigated as a function of annealing temperature. The spectrophotometric measurements of transmittance spectra were carried out in the wavelength range 200–1000 nm. Results showed that transmittance values of ZnPc thin films were increased as a result of heat treatment.
Sea-Based Infrared Scene Interpretation by Background Type Classification and Coastal Region Detection for Small Target Detection

Sea-based infrared search and track (IRST) is important for homeland security by detecting missiles and asymmetric boats. This paper proposes a novel scheme to interpret various infrared scenes by classifying the infrared background types and detecting the coastal regions in omni-directional images. The background type or region-selective small infrared target detector should be deployed to maximize the detection rate and to minimize the number of false alarms. A spatial filter-based small target detector is suitable for identifying stationary incoming targets in remote sea areas with sky only. Many false detections can occur if there is an image sector containing a coastal region, due to ground clutter and the difficulty in finding true targets using the same spatial filter-based detector. A temporal filter-based detector was used to handle these problems. Therefore, the scene type and coastal region information is critical to the success of IRST in real-world applications. In this paper, the infrared scene type was determined using the relationships between the sensor line-of-sight (LOS) and a horizontal line in an image. The proposed coastal region detector can be activated if the background type of the probing sector is determined to be a coastal region. Coastal regions can be detected by fusing the region map and curve map. The experimental results on real infrared images highlight the feasibility of the proposed sea-based scene interpretation. In addition, the effects of the proposed scheme were analyzed further by applying region-adaptive small target detection.
Chapter 2 Differentiation of Murine Myeloid-Derived Suppressor Cells

Myeloid-derived suppressor cells (MDSCs) are frequently defined as a heterogeneous population of immature cells belonging to the myeloid lineage which possess strong immunosuppressive activities. These cells ultimately derive from myeloid progenitors mainly present in the bone marrow that undergo a dysregulated differentiation pathway, ending up with the systemic mobilization of MDSCs of “monocytic” or “granulocytic” characteristics. Here we will review the current knowledge on MDSC differentiation mostly in murine cancer models, and reflect on whether MDSCs represent a unique, well-defined distinct myeloid lineage or just immature stages of myeloid cells.
Unstable tandem gene amplification generates heteroresistance (variation in resistance within a population) to colistin in Salmonella enterica

Heteroresistance, a phenomenon where subpopulations of a bacterial isolate exhibit different susceptibilities to an antibiotic, is a growing clinical problem where the underlying genetic mechanisms in most cases remain unknown. We isolated colistin resistant mutants in Escherichia coli and Salmonella enterica serovar Typhimurium at different concentrations of colistin. Genetic analysis showed that genetically stable pmrAB point mutations were responsible for colistin resistance during selection at high drug concentrations for both species and at low concentrations for E. coli. In contrast, for S. Typhimurium mutants selected at low colistin concentrations, amplification of different large chromosomal regions conferred a heteroresistant phenotype. All amplifications included the pmrD gene, which encodes a positive regulator that up‐regulates proteins that modify lipid A, and as a result increase colistin resistance. Inactivation and over‐expression of the pmrD gene prevented and conferred resistance, respectively, demonstrating that the PmrD protein is required and sufficient to confer resistance. The heteroresistance phenotype is explained by the variable gene dosage of pmrD in a population, where sub‐populations with different copy number of the pmrD gene show different levels of colistin resistance. We propose that variability in gene copy number of resistance genes can explain the heteroresistance observed in clinically isolated pathogenic bacteria.
Trafficking and Functions of Bioactive Sphingolipids: Lessons from Cells and Model Membranes

Ceramide and sphingosine and their phosphorylated counterparts are recognized as “bioactive sphingolipids” and modulate membrane integrity, the activity of enzymes, or act as ligands of G protein-coupled receptors. The subcellular distribution of the bioactive sphingolipids is central to their function as the same lipid can mediate diametrically opposite effects depending on its location. To ensure that these lipids are present in the right amount and in the appropriate organelles, cells employ selective lipid transport and compartmentalize sphingolipid-metabolizing enzymes to characteristic subcellular sites. Our knowledge of key mechanisms involved in sphingolipid signaling and trafficking has increased substantially in the past decades—thanks to advances in biochemical and cell biological methods. In this review, we focus on the bioactive sphingolipids and discuss how the combination of studies in cells and in model membranes have contributed to our understanding of how they behave and function in living organisms.
Recent advances in protein engineering and biotechnological applications of glutathione transferases

Abstract Glutathione transferases (GSTs, EC 2.5.1.18) are a widespread family of enzymes that play a central role in the detoxification, metabolism, and transport or sequestration of endogenous or xenobiotic compounds. During the last two decades, delineation of the important structural and catalytic features of GSTs has laid the groundwork for engineering GSTs, involving both rational and random approaches, aiming to create new variants with new or altered properties. These approaches have expanded the usefulness of native GSTs, not only for understanding the fundamentals of molecular detoxification mechanisms, but also for the development medical, analytical, environmental, and agricultural applications. This review article attempts to summarize successful examples and current developments on GST engineering, highlighting in parallel the recent knowledge gained on their phylogenetic relationships, structural/catalytic features, and biotechnological applications.
MiR-146a negatively regulates dectin-1-induced inflammatory responses

Dectin-1 is the critical sensor for β-glucan from Candida which is the most common human fungal pathogen and cause superficial and system infection. MicroRNAs (miRNAs) play crucial roles in regulating innate immunity. However, the functional role of miRNAs in inflammatory response dependent on the activation of dectin-1 pathway has not been defined. In the present study, we found insoluble β-glucan from the cell wall of Candida albicans (CaIG) was able to increase the production of of IL-6 and TNFα through Dectin-1-Syk-NF-κB and p38MAPK pathway. MiRNAs profiles combined with real-time PCR validation revealed that miR-146a, miR-30-5p, miR-210-3p expression level were increased in THP-1 cells treated with CaIG. The interaction between Dectin-1 and CaIG resulted in an long lasting increase of miR-146a expression dependent on Dectin-1-Syk-NF-κB, p38MAPK, contrasting with a rapid and transient increase of IL-6 and TNFα. Overexpression of miR-146a significantly suppressed the production of IL-6 and TNFα. MiR-146a mimics inhibited CaIG-induced activity of p-IκBα and translocation of NF-κB p65. Luciferase reporter assays showed miR-146a inhibited NF-κB promoter-binding activity. Together, our data suggest miR-146a may play the potent negative feedback regulator in inflammatory response following Dectin-1 stimulation.
Gene microarray analysis of lncRNA and mRNA expression profiles in patients with high-grade ovarian serous cancer

High-grade ovarian serous cancer is known for its high rates of invasion and metastasis, and resultant high mortality rate. Therefore, research concerning biomarkers and underlying molecular mechanisms of high-grade ovarian serous cancer progression and prognosis are urgently required. Long non-coding RNAs (lncRNAs) have been the subject of an increasing number of studies, and certain lncRNAs have been demonstrated to serve an important function in the development and progression of various cancers, including HOX transcript antisense RNA, competing endogenous lncRNA 2 for microRNA let-7b, urothelial cancer associated 1, and H19, imprinted maternally expressed transcript (non-protein coding). However, few studies have investigated the differential expression of lncRNAs in high-grade ovarian serous cancer. In the present study, differences in lncRNA and mRNA expression profiles between high-grade ovarian serous cancer tissue samples and healthy fallopian tube tissue samples were investigated using microarray analysis, and the differential expression of lncRNAs and mRNAs was confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Then, five abnormally expressed lncRNAs were selected, and the associations between these lncRNAs and ovarian cancer clinicopathological parameters were examined using RT-qPCR. The expression profiles of certain lncRNAs and mRNAs were confirmed to be altered between high-grade ovarian serous cancer tissues and healthy fallopian tube tissues. Furthermore, the expression levels of selected lncRNAs were associated with International Federation of Gynecology and Obstetrics stage and lymph node metastasis. These lncRNAs and mRNAs may therefore be involved in the pathogenesis of high-grade ovarian serous cancer. The results of the present study provide an experimental foundation for further exploration of the value of these lncRNAs and mRNAs in the early diagnosis and treatment of high-grade ovarian serous cancer.