Miracle-dz/UniFAR-FTUs huggingface dataset:
Yeast mitochondrial RNase P, RNase Z and the RNA degradosome are part of a stable supercomplex

Initial steps in the synthesis of functional tRNAs require 5′- and 3′-processing of precursor tRNAs (pre-tRNAs), which in yeast mitochondria are achieved by two endonucleases, RNase P and RNase Z. In this study, using a combination of detergent-free Blue Native Gel Electrophoresis, proteomics and in vitro testing of pre-tRNA maturation, we reveal the physical association of these plus other mitochondrial activities in a large, stable complex of 136 proteins. It contains a total of seven proteins involved in RNA processing including RNase P and RNase Z, five out of six subunits of the mitochondrial RNA degradosome, components of the fatty acid synthesis pathway, translation, metabolism and protein folding. At the RNA level, there are the small and large rRNA subunits and RNase P RNA. Surprisingly, this complex is absent in an oar1Δ deletion mutant of the type II fatty acid synthesis pathway, supporting a recently published functional link between pre-tRNA processing and the FAS II pathway—apparently by integration into a large complex as we demonstrate here. Finally, the question of mt-RNase P localization within mitochondria was investigated, by GFP-tracing of a known protein subunit (Rpm2p). We find that about equal fractions of RNase P are soluble versus membrane-attached.
Secondary spinal cord hypoperfusion of circumscribed areas after injury in rats

Abstract Objectives: The evaluation of the spatial spread of ischemia following spinal cord injury (SCI) is important for planning therapeutic strategies for secondary injury. The purpose of this study was to investigate in detail the change in regional spinal cord blood flow (rSCBF) after SCI. Methods: Thirty-four male Wistar rats were used, for which laminectomies of the T11–13 vertebrae were performed. SCI was produced by a directed impact through a laminectomy site at the level of the Th12 using a pneumatic impact device. We measured the sequential and spatial changes of rSCBF using a laser Doppler scanning technique before and after SCI in rats not only at the injured myelomere but also at the circumferent myelomeres. SCBF mapping was carried out before and after SCI on each site. Results: After SCI, the rSCBF value gradually decreased for each site for the SCI group (n=26), while it globally decreased at the epicenter. Moreover, a decrease in SCBF was observed at the caudal and rostral sites. The mean value of the %SCBF 120 minutes after SCI for each site was 63.6±2.3% (Th11), 74.4±4.5% (Th12), 75.8±3.2% (Th13), and was significantly lower for the rostral site compared with the caudal site (p<0.05, one-way analysis of variance). Discussion: This study found that SCBF is significantly decreased not only at the injured myelomere but also at the circumferent myelomeres. Circumferentially extending ischemia after SCI is related to secondary injury after SCI. The improvement in SCBF after SCI, therefore, can be attributed to the treatment of SCI.
Calculating credit risk capital charges with the one-factor model

Even in the simple one-factor credit portfolio model that underlies the Basel II regulatory capital rules coming into force in 2007, the exact contributions to credit value-at-risk can only be calculated with Monte-Carlo simulation or with approximation algorithms that often involve numerical integration. As this may require a lot of computational time, there is a need for approximate analytical formulae. In this note, we develop formulae according to two different approaches: the granularity adjustment approach initiated by M. Gordy and T. Wilde, and a semi-asymptotic approach. The application of the formulae is illustrated with a numerical example. Keywords: One-factor model, capital charge, granularity adjustment, quantile derivative.
Sorafenib Hepatobiliary Disposition: Mechanisms of Hepatic Uptake and Disposition of Generated Metabolites

Sorafenib is an orally active tyrosine kinase inhibitor used in the treatment of renal and hepatocellular carcinoma. This study was designed to establish whether transport proteins are involved in the hepatic uptake of sorafenib and to determine the extent of biliary excretion of sorafenib and its metabolites in human hepatocytes. Initial uptake was assessed in freshly isolated, suspended human hepatocytes in the presence of inhibitors and modulators. [14C]Sorafenib (1 µM) uptake at 4°C was reduced by about 61–63% of the uptake at 37°C, suggesting a high degree of passive diffusion. Hepatocyte uptake of [14C]sorafenib was not Na+ dependent or influenced by the organic anion transporter 2 inhibitor ketoprofen. However, initial [14C]sorafenib hepatocyte uptake was reduced by 46 and 30% compared with control values in the presence of the organic anion transporting polypeptide inhibitor rifamycin SV and the organic cation transporter (OCT) inhibitor decynium 22, respectively. [14C]Sorafenib (0.5–5 µM) uptake was significantly higher in hOCT1-transfected Chinese hamster ovary cells compared with mock cells, and inhibited by the general OCT inhibitor, 1-methyl-4-phenylpryidinium. OCT1-mediated uptake was saturable with a Michaelis-Menten constant of 3.80 ± 2.53 µM and a Vmax of 116 ± 42 pmol/mg/min. The biliary excretion index and in vitro biliary clearance of sorafenib (1 µM) in sandwich-cultured human hepatocytes were low (∼11% and 11 ml/min/kg, respectively). Results suggest that sorafenib uptake in human hepatocytes occurs via passive diffusion, by OCT1, and by organic anion transporting polypeptide(s). Sorafenib undergoes modest biliary excretion, predominantly as a glucuronide conjugate(s).
Estimating the Probability of Informed Trading - Does Trade Misclassification Matter?

A method of enhancing the growth of an animal is provided. The method includes causing the animal to ingest or absorb an effective amount of one or more Fe III complex compounds, including but not limited to Fe III complexes comprising ligands bound to the iron center selected from amino acids or α-hydroxy acids, o-hydroxy benzoic acids or pyridine-2-carboxylic acids, such as ferric quinate, ferric tyrosine, ferric DOPA and ferric phenylalanine. Compounds which are structural and/or functional variants, derivatives and/or analogs of the foregoing compounds, as further described herein are also disclosed. Methods for inhibiting, reducing, or preventing biofilm formation or buildup on a surface; the treatment of, inhibition of growth of, and inhibition of colonization by, bacteria, both in biological and non-biological environments; disinfecting surfaces, potentiating the effects of antibiotics and other anti-microbial agents, and increasing the sensitivity of bacteria and other microorganisms, to anti-microbial agents are also provided.
Towards a Functional System Architecture for Automated Vehicles

This paper presents a functional system architecture for an automated vehicle. It provides an overall, generic structure that is independent of a specific implementation of a particular vehicle project. Yet, it has been inspired and cross-checked with a real world automated driving implementation in the Stadtpilot project at the Technische Universitat Braunschweig. The architecture entails aspects like environment and self perception, planning and control, localization, map provision, Vehicle-To-X-communication, and interaction with human operators.
Extension du domaine de la stratégie

The purpose of this article is to specify the nature of a critical activity in the field of strategy. While post-procedural researches offered alternatives to conventional strategy, they do not really fit to a critical theory of strategic management. Building on a literature review in critical management studies and strategy, our article calls for a critical research agenda in strategic management, that goes beyond the post-rational perspective in the field.
Prevalence and distribution of human herpesvirus 6 variants A and B in adult human brain

The presence of human herpesvirus 6 (HHV‐6) in brain tissues of 40 consecutive post‐mortem cases was examined. For each case, autopsy samples were collected from the cerebellum, frontal, temporal, parietal and occipital lobes of both sides of the brain. HHV‐6 DNA was detected by nested polymerase chain reaction and characterised into variants A and B. Overall, 97/400 (24.3%) samples were positive for HHV‐6 DNA with 16 being variant A and 81 being variant B, but none of the samples harboured both variants. When analysed by patient, 34/40 (85%) had HHV‐6 DNA detected in the brain. The viral DNA positivity did not show significant variation with gender and age. Four patients harboured variant A, 23 harboured variant B, and seven had both variants at different positions. The results indicate that both HHV‐6A and HHV‐6B are neurotropic and human brain may be another site for latency. HHV‐6B was detected in brain tissues of a majority (75%) of the studied population and with a widespread distribution within the brain. Although the observed prevalence of HHV‐6A in brain is lower (27.5%), in view of its lower seroprevalence, the neuroinvasive potential of variant A may be comparable to that of variant B. Although both variants are potential pathogens for the nervous system, the fact that they can exist, probably for most of the time, as commensals in human brain needs to be considered when interpreting their roles in neuropathology. J. Med. Virol. 64:42–46, 2001. © 2001 Wiley‐Liss, Inc.
Isolation, Characterization, and Cloning of α-l-Arabinofuranosidase Expressed during Fruit Ripening of Japanese Pear

α-l-Arabinofuranosidase (α-l-arafase) was purified from fruit of Japanese pear (Pyrus pyrifolia). The enzyme solubilized from the cell wall by NaCl and Triton X-100 had the homogeneity of a single 62-kD polypeptide on SDS-PAGE after purification through the steps of hydroxyapatite, anion-exchange chromatography, and size-exclusion chromatography. A related cDNA clone was isolated (PpARF2). The transcript and related protein were detected solely in the ripening fruit corresponding to the increase of α-l-arafase activity. Transcripts of PpARF2 were not detected in buds, leaves, roots, or shoots of the Japanese pear. The deduced amino acid sequences of PpARF2 had low identity with those of other plants or bacteria. This α-l-arafase belonged to glycoside hydrolase family 3, which includes some β-xylosidases. The purified enzyme hydrolyzed mainly p-nitrophenyl α-l-arabinofuranoside and also reacted bifunctionally with p-nitrophenyl β-d-xylopyranoside. However, it released only arabinose from native cell wall polysaccharides prepared from Japanese pear and from sugar beet arabinan. The enzyme did not release xylose from arabinoxylan and xylan. The only activity of the α-l-arafase presented here was hydrolyzing the arabinosyl residue from native polysaccharides, whereas it showed bifunctional activity against artificial substrates. According to the expression pattern and properties of the enzyme, it is a new member of the glycoside hydrolase family 3 isolated from fruit, and it may be responsible for modification of the cell wall architecture during fruit softening.
Security (Hyper-)properties in Workflow Systems: From Specification to Verification

Provable security guarantees for software systems are highly desirable. Our work aims at improving and integrating existing formal verification techniques into a framework for the specification and verification of typical security requirements of large-scale, distributed workflow systems. Challenges include the uniform modelling of different types of security requirements, the decomposition of global security requirements into requirements on subcomponents, and the refinement of an abstract specification towards an implementation. We focus our attention on workflow management systems due to their interesting security requirements and the widespread use of model-driven techniques in this area (e.g. using BPMN diagrams). We build upon existing verification techniques for a specific notion of information flow security, and intend to apply our results to concrete example systems such as a secure web-based conference management system.
Th ird ventricular chordoid meningioma or chordoma : a diagnostic dilemma based on a single case

We report a case and literature review of a rare third ventricular tumor in a child with histologic characteristics of chordoma and chordoid meningioma (CM). Case: A 13-year-old boy was diagnosed with a recurrent intraventricular tumor 22 months aft er complete surgical removal. Reoperation was indicated; treatment consisted of total microsurgical removal, histologic and immunohistochemical classifi cation, and follow up. Literature review on Pub Med was performed using the Mesh key words: “Cerebral Ventricle Neoplasms” AND “Pediatrics”. Histologic and immunohistochemical analysis aft er the fi rst operation had shown chordoma, and second histologic and extended immunohistochemical analysis showed positivity for D2-40 marker, which is negative in chordoma but can be positive in CM. It was concluded to be a case of CM. Fourteen months aft er reoperation there were no signs of tumor recurrence. Literature review showed two cases of intraventricular CM, one situated in lateral ventricle and the other in third ventricle. Conclusion: Th is is the second reported case of CM situated in the third ventricle in a pediatric patient. In this case, follow up was performed regularly for 22 months aft er the fi rst complete resection, when the tumor recurred. First histologic and immunohistochemical analysis showed chordoma, whereas second analysis showed CM. Fourteen months aft er reoperation there were no signs of tumor relapse. Th e boy returned to his everyday activities, with some hormone misbalance treated with hormone substitutes.
18 Claudins in Normal and Lung Cancer State

The tight junctions are critical for the sealing of cellular sheets and controlling paracellular ion flux. Tight junctions are composed primarily of 3 components: The IgG-like family of junctional adhesion molecules (JAMs), occluding and claudin families. Claudins are the main constituents of tight junctions. The claudin family proteins is composed of approximately 24 transmembrane proteins, all of which are closely related, most of them are well characterized at the level of gene and protein. The claudins are present in variety of normal tissues, hyperplastic conditions, but have also been found in benign neoplams and cancers that exhibit epithelial differentiation. Loss of claudins expression has been reported in various malignant diseases. The differential expression of several members of the family of the claudins in various cancers has been used to confirm the histological identity of certain types of cancer.
Hearing loss and cognitive effort in older adults' report accuracy for verbal materials.

BACKGROUND In addition to declines in auditory acuity, adult aging is often also accompanied by reduced cognitive efficiency, most notably in working memory resources and a general slowing in a number of perceptual and cognitive domains. Effectiveness of speech comprehension by older adults reflects a balance between these declines and the relative preservation in healthy aging of linguistic knowledge and the procedural rules for its application. PURPOSE To examine effects of hearing acuity in older adults on intelligibility functions for sentences that varied in two degrees of syntactic complexity, with their concomitant demands on older adults' working memory resources. RESEARCH DESIGN Stimuli consisted of monosyllabic words presented in isolation, and nine-word sentences that varied in syntactic complexity. Two sentence types were employed: sentences with a subject-relative clause structure, and more syntactically complex sentences in which meaning was expressed with an object-relative clause structure. The stimuli were presented initially below the level of audibility and then increased in loudness in 2 dB increments until the single-word stimuli and all nine words of the sentence stimuli could be correctly reported. STUDY SAMPLE Participants were 16 older adults with good hearing acuity for their ages, 16 age-matched adults with mild-to-moderate hearing loss, and 16 young adults with age-normal hearing. RESULTS Along with confirming better report accuracy for the words of meaningful sentences than for words heard in isolation, performance curves for the sentence stimuli showed a significant effect of syntactic complexity. This took the form of older adults having poorer report accuracy at any given loudness level for sentences with greater syntactic complexity. This general effect of syntactic complexity on perceptual report accuracy was further exacerbated by age and hearing loss. CONCLUSIONS Age-limited working memory resources are impacted both by the resource demands required for comprehension of syntactically complex sentences and by effortful listening attendant to hearing loss.
The Value of Third-Party Certification of Preconditioning Claims at Iowa Feeder Cattle Auctions

After controlling a variety of feeder cattle characteristics and market and sale conditions in Iowa feeder auctions, the price premiums for preconditioning claims (vaccinations and minimum 30 days of weaning) with and without third-party certification (TPC) are estimated as $6.12/cwt and $3.35/cwt, respectively. These premiums differ statistically (p < 0.0001), and their difference exceeds the average participation cost of TPC ($l/cwt). This indicates that TPC is valued in the market to credibly signal preconditioning investment under asymmetric information.
Implementation of Support Vector Machines for Classification of Clinical Datasets

In this paper, Principal Component Analysis is used for feature extraction, and a statistical learning based Support Vector Machine is designed for functional classification of clinical data. Appendicitis data collected from BHEL Hospital, Trichy is taken and classified under three classes. Feature extraction transforms the data in the high-dimensional space to a space of fewer dimensions. The classification is done by constructing an optimal hyperplane that separates the members from the nonmembers of the class. For linearly nonseparable data, Kernel functions are used to map data to a higher dimensional space and there the optimal hyperplane is found. This paper works with different SVMs based on radial basis and polynomial kernels, and their performances are compared.
Dexterous Aerial Robots—Mobile Manipulation Using Unmanned Aerial Systems

In this paper, we present selected benchmark aerial manipulation tasks using an aerial vehicle endowed with multi-degree of freedom manipulators. The proposed tasks analyze environmental coupling and are broken into three general categories: momentary, loose, and strong coupling. A classical control structure is derived, tuned, and verified through experiments, conducted for benchmarking purposes to include pick-and-place, insertion, and valve-turning tasks. Although other nonlinear controllers may prove more effective, the classical control approach has been selected in order to analyze contact stability and provide benchmark results for future reference. An analysis of system stability is conducted and implemented into the controller. A vision-based high-level controller fuses motion tracking data in order to provide control of both the aircraft and the manipulators, allowing the system to become coupled to the environment and perform the required operation. We present recent results validating our framework using the proposed aircraft-arm system.
A STUDY OF INDONESIAN SLANG IN TWO LUPUS MOVIES

This study aims to investigate the Indonesian Slang used in the two Indonesian movies, entitled Lupus and to reveal the commonalities and differences of Slang usage in the different time slot movies. The data are the spoken slang in the two movies; Lupus: Kejar Daku Kau Kujitak (1987) and Bangun lagi dong Lupus! (2013). Furthermore, the data are classified into Mattielo’s (2008) types of slang and O’Grady and Guzman’s (1996) word-formation theories, complemented by Hatch and Brown’s (1995) to seek the commonalities and differences of the slang. The findings reveal that the types of slang in both movies are age and localism restricted type and unconventionality type. In addition, the word formation process of slang that mostly occurrs are borrowing, clipping, affixation in Lupus 1987 and borrowing, affixation and coinage in Lupus 2013. In conclusion, Indonesian slang, according to its speakers and its functions, is still mostly used by teenagers and identical with Betawinese, a regional language. In contrast, due to the time difference of the movie, the development of information and technology influences slang used in the latest movie, especially borrowing English, and the slang is also enriched by many new term creations. ;---Penelitian ini bertujuan untuk meneliti penggunaan slang bahasa Indonesia di dalam dua film, berjudul Lupus, dan untuk mengungkapkan persamaan dan perbedaan penggunaan slang bahasa Indonesia di dalam film yang berbeda masa. Data penelitian merupakan slang yang diucapkan di dalam dua film; Lupus: Tangkaplah Daku, Kau Kujitak (1987) and Bangun lagi dong Lupus! (2013). Kemudian diklasifikasikan ke dalam jenis slang teori Mattielo (2008) dan pembentukkan kata teori O’Grady dan Guzman (1996) dilengkapi oleh teori Hatch dan Brown (1995) untuk mencari persamaan dan perbedaannya. Temuan mengungkapkan bahwa jenis slang di kedua film adalah tipe age and localism restricted “terbatas pada usia dan domisili” dan jenis unconventionality “inkonvensional”. Sebagai tambahan, pembentukkan kata yang paling sering muncul adalah, borrowing, clipping dan affixation di dalam film Lupus 1987 dan borrowing, affixation, dan coinage di dalam film Lupus 2013. Kesimpulannya, slang bahasa Indonesia menurut penutur dan fungsinya kebanyakan masih digunakan oleh remaja dan identik dengan dialek Betawi. Sebaliknya, dengan adanya perbedaan waktu diantara kedua film, perkembangan informasi dan teknologi mempengaruhi pengguanaan slang pada film terbaru, khususnya penyerapan bahasa Inggris dan slang juga diperkaya dengan banyaknya kreasi istilah baru.
A PLENA-like gene of peach is involved in carpel formation and subsequent transformation into a fleshy fruit

MADS-box genes have been shown to play a role in the formation of fruits, both in Arabidopsis and in tomato. In peach, two C-class MADS-box genes have been isolated. Both of them are expressed during flower and mesocarp development. Here a detailed analysis of a gene that belongs to the PLENA subfamily of MADS-box genes is shown. The expression of this PLENA-like gene (PpPLENA) increases during fruit ripening, and its ectopic expression in tomato plants causes the transformation of sepals into carpel-like structures that become fleshy and ripen like real fruits. Interestingly, the transgenic berries constitutively expressing the PpPLENA gene show an accelerated ripening, as judged by the expression of genes that are important for tomato fruit ripening. It is suggested that PpPLENA might interfere with the endogenous activity of TAGL1, thereby activating the fruit ripening pathway earlier compared with wild-type tomato plants.