Miracle-dz/UniFAR-FTUs huggingface dataset:
A cyclin D1/microRNA 17/20 regulatory feedback loop in control of breast cancer cell proliferation

Decreased expression of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. Herein, levels of the miR-17-5p/miR-20a miRNA cluster were inversely correlated to cyclin D1 abundance in human breast tumors and cell lines. MiR-17/20 suppressed breast cancer cell proliferation and tumor colony formation by negatively regulating cyclin D1 translation via a conserved 3′ untranslated region miRNA-binding site, thereby inhibiting serum-induced S phase entry. The cell cycle effect of miR-17/20 was abrogated by cyclin D1 siRNA and in cyclin D1–deficient breast cancer cells. Mammary epithelial cell–targeted cyclin D1 expression induced miR-17-5p and miR-20a expression in vivo, and cyclin D1 bound the miR-17/20 cluster promoter regulatory region. In summary, these studies identify a novel cyclin D1/miR-17/20 regulatory feedback loop through which cyclin D1 induces miR-17-5p/miR-20a. In turn, miR-17/20 limits the proliferative function of cyclin D1, thus linking expression of a specific miRNA cluster to the regulation of oncogenesis.
Governança corporativa voltada à Produção Mais Limpa: influência dos stakeholders Corporate governance focused on cleaner production: the influence of stakeholders

This study aims to evaluate the influence of stakeholders in determining a Corporate Governance focused on Cleaner Production (CP). First, three constructs that influence the governance of enterprises to implement CP were identified in the literature: public policy, economic agents, and society. After that, a survey was carried out in companies associated with the Ethos Institute. Regarding statistical analysis, principal components analysis was conducted for categorical or nominal data. The results showed some trends in terms of stakeholders' influences, directing changes in the principles of corporate governance, which consequently boost endogenous and incremental changes in the production system for the deployment of CP. Public policy tends to tax carbon emissions from companies and promote low-interest financing for investment in clean technologies and production control in the production system; the economic agent tends to become aware of and comply with environmental laws that lead to implantation of CP; and society tends to buy green products and report deceptive business practices. Resumo: Este estudo visa avaliar as influencias dos stakeholders na determinacao de uma Governanca Corporativa voltada a Producao Mais Limpa (P+L). Primeiramente identificaram-se na literatura tres constructos que exercem influencia sobre a governanca da empresa para a implementacao da P+L, que sao: politica publica, agentes economicos e sociedade. Depois disso, realizou-se um survey em empresas associadas ao Instituto Ethos. Para a avaliacao estatistica, utilizou-se a analise de componentes principais para dados categoricos ou nominais. Os resultados apontaram algumas tendencias em termos de influencias dos stakeholders , direcionando a mudancas de principios da governanca corporativa, que conseguinte impulsionam mudancas endogenas e incrementais no sistema de producao para a implantacao da P+L. A politica publica tende a taxar emissoes de carbono das empresas e promover financiamento a juros baixos para investimento em tecnologias limpas e controle da producao no sistema de producao, o agente economico tende a se conscientizar e obedecer a leis ambientais que levam a implantacao de P+L, e a sociedade tende a comprar produtos ecologicos, alem de denunciar praticas enganosas das empresas. Palavras-chave: Producao Mais Limpa. Governanca corporativa. Politica publica. Economia solidaria. Responsabilidade social.
Temporal signatures of processing voiceness and emotion in sound

Abstract This study explored the temporal course of vocal and emotional sound processing. Participants detected rare repetitions in a stimulus stream comprising neutral and surprised non-verbal exclamations and spectrally rotated control sounds. Spectral rotation preserved some acoustic and emotional properties of the vocal originals. Event-related potentials elicited to unrepeated sounds revealed effects of voiceness and emotion. Relative to non-vocal sounds, vocal sounds elicited a larger centro-parietally distributed N1. This effect was followed by greater positivity to vocal relative to non-vocal sounds beginning with the P2 and extending throughout the recording epoch (N4, late positive potential) with larger amplitudes in female than in male listeners. Emotion effects overlapped with the voiceness effects but were smaller and differed topographically. Voiceness and emotion interacted only for the late positive potential, which was greater for vocal-emotional as compared with all other sounds. Taken together, these results point to a multi-stage process in which voiceness and emotionality are represented independently before being integrated in a manner that biases responses to stimuli with socio-emotional relevance.
Monosynaptic functional connectivity in cerebral cortex during wakefulness and under graded levels of anesthesia

The balance between excitation and inhibition is considered to be of significant importance for neural computation and cognitive function. Excitatory and inhibitory functional connectivity in intact cortical neuronal networks in wakefulness and graded levels of anesthesia has not been systematically investigated. We compared monosynaptic excitatory and inhibitory spike transmission probabilities using pairwise cross-correlogram (CCG) analysis. Spikes were measured at 64 sites in the visual cortex of rats with chronically implanted microelectrode arrays during wakefulness and three levels of anesthesia produced by desflurane. Anesthesia decreased the number of active units, the number of functional connections, and the strength of excitatory connections. Connection probability (number of connections per number of active unit pairs) was unaffected until the deepest anesthesia level, at which a significant increase in the excitatory to inhibitory ratio of connection probabilities was observed. The results suggest that the excitatory–inhibitory balance is altered at an anesthetic depth associated with unconsciousness.
Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens

We report the identification of two novel minor histocompatibility antigens (mHAgs), encoded by two separate single nucleotide polymorphisms on a single gene, BCL2A1, and restricted by human histocompatibility leukocyte antigen (HLA)-A*2402 (the most common HLA-A allele in Japanese) and B*4403, respectively. Two cytotoxic T lymphocyte (CTL) clones specific for these mHAgs were first isolated from two distinct recipients after hematopoietic cell transplantation. Both clones lyse only normal and malignant cells within the hematopoietic lineage. To localize the gene encoding the mHAgs, two-point linkage analysis was performed on the CTL lytic patterns of restricting HLA-transfected B lymphoblastoid cell lines obtained from Centre d'Etude du Polymorphisme Humain. Both CTL clones showed a completely identical lytic pattern for 4 pedigrees and the gene was localized within a 3.6-cM interval of 15q24.3–25.1 region that encodes at least 46 genes. Of those, only BCL2A1 has been reported to be expressed in hematopoietic cells and possess three nonsynonymous nucleotide changes. Minigene transfection and epitope reconstitution assays with synthetic peptides identified both HLA-A*2402– and B*4403-restricted mHAg epitopes to be encoded by distinct polymorphisms within BCL2A1.
An open-source 3D printed underactuated robotic gripper

In this paper authors present an open source low-cost basic robotic end effector platform for facilitating research on robotic grasping. The 3D design model of a three fingered underactuated robotic gripper is presented and manufactured with minimal number of 3D printed components and an off-the-shelf servomotor actuator. An underactuated finger, gear train mechanisms and an overall gripper assembly design are described in details followed by illustration and discussion of grasping of objects with various geometries. The presented open source gripper design will be released for downloading on the authors' research lab web-site www.alaris. kz and can be useful for robotic researchers as a platform to build their own robotic end effector solutions for research and educational purposes.
Deep-Water Carbonate Ion Concentrations in the Western Tropical Pacific Since the Mid-Pleistocene: A Major Perturbation During the Mid-Brunhes

, Abstract We present a new deep-water carbonate ion concentration ([CO 32 (cid:1) ]) record, reconstructed from the “ size-normalized weight ” of the planktonic foraminifer Neogloboquadrina dutertrei in core MD06-3047B, representing a middepth site (2.5 km) in the western tropical Paci ﬁ c since 700 ka. On glacial-interglacial time scales, deep-water [CO 32 (cid:1) ] exhibits an inverse relationship with global sea-level elevations, consistent with the “ coral reef hypothesis ” that the deep Paci ﬁ c carbonate system responded to variations in shelf-carbonate production through the past 700 kyr. On longer time scales, a decoupling between deep-water [CO 32 (cid:1) ] and δ 13 C around the globe can be explained by a combination of continental weathering and nutrient inputs. During the mid-Brunhes interval (~600 – 200 ka), [CO 32 (cid:1) ] reached a maximum of ~100 μ mol kg (cid:1) 1 at the marine isotope stage (MIS) 12/11 boundary, followed by a steep decrease to a minimum of ~40 μ mol kg (cid:1) 1 during middle MIS 11, representing the largest-amplitude change in [CO 32 (cid:1) ] over the past 700 kyr. The [CO 32 (cid:1) ] maximum records the largest deglacial oceanic carbon release since 700 ka, and the [CO 32 (cid:1) ] minimum was a response to a global increase in pelagic carbonate production. From MIS 3 to 2 and from early to middle MIS 13, [CO 32 (cid:1) ] showed rising trends opposite to those at water depths greater than 3.4 km, implying enhanced Paci ﬁ c strati ﬁ cation during these intervals. These ﬁ ndings provide new insights into the Pleistocene evolution of the carbonate system in the Paci ﬁ c Ocean.
Deep sea underwater robotic exploration in the ice-covered Arctic ocean with AUVs

The Arctic seafloor remains one of the last unexplored areas on Earth. Exploration of this unique environment using standard remotely operated oceanographic tools has been obstructed by the dense Arctic ice cover. In the summer of 2007 the Arctic Gakkel Vents Expedition (AGAVE) was conducted with the express intention of understanding aspects of the marine biology, chemistry and geology associated with hydrothermal venting on the section of the mid-ocean ridge known as the Gakkel Ridge. Unlike previous research expeditions to the Arctic the focus was on high resolution imaging and sampling of the deep seafloor. To accomplish our goals we designed two new Autonomous Underwater Vehicles (AUVs) named Jaguar and Puma, which performed a total of nine dives at depths of up to 4062m. These AUVs were used in combination with a towed vehicle and a conventional CTD (conductivity, temperature and depth) program to characterize the seafloor. This paper describes the design decisions and operational changes required to ensure useful service, and facilitate deployment, operation, and recovery in the unique Arctic environment.
A variant of the multi-agent rendezvous problem

The classical multi-agent rendezvous problem asks for a deterministic algorithm by which $n$ points scattered in a plane can move about at constant speed and merge at a single point, assuming each point can use only the locations of the others it sees when making decisions and that the visibility graph as a whole is connected. In time complexity analyses of such algorithms, only the number of rounds of computation required are usually considered, not the amount of computation done per round. In this paper, we consider $\Omega(n^2 \log n)$ points distributed independently and uniformly at random in a disc of radius $n$ and, assuming each point can not only see but also, in principle, communicate with others within unit distance, seek a randomised merging algorithm which asymptotically almost surely (a.a.s.) runs in time $O(n)$, in other words in time linear in the radius of the disc rather than in the number of points. Under a precise set of assumptions concerning the communication capabilities of neighboring points, we describe an algorithm which a.a. s. runs in time $O(n)$ provided the number of points is $o(n^3)$. Several questions are posed for future work.
The role of the fusiform-amygdala system in the pathophysiology of autism.

CONTEXT Autism is a condition of unknown origin with well-documented impairments in social perception and cognition. OBJECTIVE To assess the relevance of the fusiform-amygdala system to the pathophysiology of autism spectrum conditions. DESIGN Cross-sectional case-control study. SETTING University hospital. PARTICIPANTS A total of 27 adults with autism spectrum conditions and 29 age-, sex-, and intelligence quotient-matched typically developed healthy controls. Patients were assessed according to DSM-IV criteria using the Autism Diagnostic Interview-Revised. INTERVENTIONS We applied an automated measurement to estimate fusiform gyrus cortical thickness and a manual tracing method to obtain amygdala volumes. We analyzed volumetric covariance among these brain regions and assessed the functional relevance of anatomical findings by analyzing correlations with emotional face-processing performance. MAIN OUTCOME MEASURES Fusiform gyrus cortical thickness, amygdala volume, emotional face processing. RESULTS We found a specific local increase in cortical thickness of the fusiform gyrus and associated impairments in face processing in individuals with autism. Anatomical covariance between amygdala volume and the increase in fusiform gyrus local thickness was significantly smaller in the group with autism spectrum conditions. CONCLUSIONS Our data provide the first anatomical evidence of an abnormal amygdala-fusiform system and its behavioral relevance to face-processing deficits in autism spectrum conditions. In light of recent evidence of the involvement of the fusiform gyrus and amygdala in social perception as well as the areas of social cognition and emotional awareness, all of which are relevant to autism, our findings might represent a core pathophysiological mechanism of autism.
Children's Oncology Group's 2013 blueprint for research: Central nervous system tumors

In the US, approximately 2,500 children are diagnosed annually with brain tumors. Their survival ranges from >90% to <10%. For children with medulloblastoma, the most common malignant brain tumor, 5‐year survival ranges from >80% (standard‐risk) to 60% (high‐risk). For those with high‐grade gliomas (HGGs) including diffuse intrinsic pontine gliomas, 5‐year survival remains <10%. Sixty‐five percent patients with ependymoma are cured after surgery and radiation therapy depending on the degree of resection and histopathology of the tumor. Phase II trials for brain tumors will investigate agents that act on cMET, PDGFRA, or EZH2 in HGG, DIPG, or medulloblastoma, respectively. Phase III trials will explore risk‐based therapy stratification guided by molecular and clinical traits of children with medulloblastoma or ependymoma. Pediatr Blood Cancer 2013; 60: 1022–1026. © 2012 Wiley Periodicals, Inc.
Physics. Quantum mechanics braces for the ultimate test.

Physicists are on a quest to build the ultimate quantum cryptography system: one that users could trust implicitly, even if they had bought it from their worst enemy. First, however, they have to plug a few stubborn holes in one of the bedrocks of modern physics: quantum mechanics. The microscopic world that quantum mechanics describes is a bizarre place where nothing is certain and the act of observation changes things. Over the past 40 years, that description has been put to the test in a series of elegant experiments that have shown it to be true. Although most physicists find the results convincing, these experiments did skirt around a few tiny loopholes by which reality could have fooled physicists into thinking that quantum mechanics paints a complete picture. It9s these loopholes that groups around the world are competing to close. The winners will have the satisfaction of settling one of the most stubborn problems in physics. As a bonus, they will also hold the key to the perfect quantum security system. * Zeeya Merali is a freelance writer based in London
Immunological crossreactivity between a cloned antigen of Onchocerca volvulus and a component of the retinal pigment epithelium

Onchocerciasis (river blindness) is a major blinding disease in Africa, Central America, and South America. Loss of vision can be due to corneal change, optic atrophy, or chorioretinal disease. It has been suggested that autoimmunological reactions resulting from crossreactivity between parasite antigens and components of eye tissues contribute to development of ocular pathology. Using sera collected from onchocerciasis patients as a screening reagent, a cDNA clone (Ov39) has been isolated from a lambda gt11 expression library of Onchocerca volvulus. This antigen exhibits immunological crossreactivity with a component of retinal pigment epithelium cells (RPE). Antiserum raised against this recombinant peptide immunoprecipitates a 22,000 Mr antigen of adult O. volvulus and recognizes a 44,000 Mr component of bovine RPE by Western blotting. A 44,000 Mr antigen of cultured human RPE metabolically labeled with 35S- methionine can be immunoprecipitated with the same antiserum. An antigen of the same size is recognized by a rabbit antiserum raised against whole O. volvulus extract. Immunocytochemical studies on cryostat sections of the bovine eye using the antirecombinant sera localizes this antigen to the RPE.
Topologically correct surface reconstruction using alpha shapes and relations to ball-pivoting

The problem to reconstruct a surface given a finite set of boundary points is of growing interest, e.g. in the context of laser range images. While a lot of heuristic methods have been published in this context (e.g. the ball-pivoting algorithm), there exist only a few algorithms which guarantee the reconstruction to be homeomorphic to the original surface if a certain sampling density is reached. However, the sampling density mentioned is in most cases much higher than what seems to be sufficient on real data. In this paper we show how recently proved results about homology extraction from surface samples can be adopted to surface reconstruction and we significantly improve the bounds on the sampling density in case of noise-free samplings. This allows us to prove for the first time that the ball-pivoting algorithm reconstructs certain object surfaces without any topological changes and we can give bounds on the reconstruction error regarding both position and normal direction of the boundary.
Deciphering a 2 Gyr‐Long Thermal History From a Multichronometer (U‐Th)/He Study of the Phalaborwa Carbonatite, Kaapvaal Craton, South Africa

(U‐Th)/He data were obtained for four minerals (baddeleyite‐BHe, titanite‐THe, zircon‐ZHe, apatite‐AHe) from the 2.06 Ga Phalaborwa carbonatite complex and nearby Archean basement of the Kaapvaal craton, South Africa. Our goals are to evaluate the relative He temperature sensitivities of these phases, better understand how radiation damage and other factors affect them, and inform aspects of the craton's thermal history. BHe dates overlap with Phalaborwa emplacement and record the highest temperatures of the dated phases. THe dates are 700–1,100 Ma and display a limited negative date‐eU correlation. ZHe dates are negatively correlated with eU, are younger (561–32 Ma) and have higher eU than the THe data, with the highest‐eU grains younger than the AHe dates. AHe dates are reproducible with a mean of 107 ± 7 Ma. The THe and ZHe data show radiation damage reduction of their He retentivities manifested as the negative date‐eU correlations. Alpha dose estimates for zircon are several orders of magnitude higher than for titanite, consistent with the ZHe dates recording lower temperatures and a younger portion of the history than the THe dates. Thermal history modeling yields time‐temperature paths that (1) explain the AHe, ZHe, and THe data while honoring existing geologic constraints, thus demonstrating the internal consistency of the data set, (2) imply possible reheating during Namaqua‐Natal orogenesis, and (3) limit maximum probable temperatures to ∼180°C during 300–183 Ma Karoo basin burial. The results show that exploiting multiple (U‐Th)/He thermochronometers and radiation damage effects can provide new insights into long‐term craton evolution.
BIOGEOGRAPHICAL AFFINITIES OF THE BUTTERFLIES OF A ' FORGOTTEN ' ISLAND : MONA ( PUERTO RICO )

David Spencer Smith Hope Entomological Collections and Department of Zoology. Oxford University, England Stuart J . Ramos Department of Biology, Mayaquez Campus, University of Puerto Rico, Mayaguez Faustino McKenzie Institute of Neurobiology, University of Puerto Rico, San Juan Eugene Munroe Grani te Hill Farm, RR 2, Dunrobin. Ontario, Canada Lee D. Miller Allyn Museum of Entomology of the Florida State Museum, 3621 Bay Shore Road, Sarasota, Florida 34234
Computability and Complexity over the Product Topology of Real Numbers

Kawamura and Cook have developed a framework for studying the computability and complexity theoretic problems over "large" topological spaces. This framework has been applied to study the complexity of the differential operator and the complexity of functionals over the space of continuous functions on the unit interval $C[0,1]$. In this paper we apply the ideas of Kawamura and Cook to the product space of the real numbers endowed with the product topology. We show that no computable norm can be defined over such topology. We investigate computability and complexity of total functions over the product space in two cases: (1) when the computing machine submits a uniformally bounded number of queries to the oracle and (2) when the number of queries submitted by the machine is not uniformally bounded. In the first case we show that the function over the product space can be reduced to a function over a finite-dimensional space. However, in general there exists functions whose computing machines must submit a non-uniform number of queries to the oracle indicating that computing over the product topology can not in general be reduced to computing over finite-dimensional spaces.