Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation

Flocculating yeasts are highly useful in fermentation processes because these cells can be separated easily from the fermentation mash. However, native yeasts are usually non-flocculating, including Kluyveromyces marxianus, which exhibits a potent high-temperature ethanol fermentation ability. We describe here the construction of flocculent K. marxianus strains via the introduction of the FLO1, FLO5, FLO9, and FLO10 genes from Saccharomyces cerevisiae. The S. cerevisiae FLO genes were overexpressed by upstream insertion of the constitutive TDH3 promoter, resulting in flocculent S. cerevisiae strains. These TDH3p-FLO sequences were then amplified by PCR and introduced directly into a K. marxianus strain. These K. marxianus strains showed a flocculation phenotype, indicating that the introduced S. cerevisiae TDH3 promoter and all FLO genes were functional in this strain. Moreover, a flocculating K. marxianus strain showed the same ethanol production profile as that of its wild-type parent. The K. marxianus flocculating strains we generated should be useful in the future development of cost-effective fed-batch and continuous fermentation systems at high temperatures.
Qualitative and Quantitative Features of Music Reported to Support Peak Mystical Experiences during Psychedelic Therapy Sessions

Psilocybin is a classic (serotonergic) hallucinogen (“psychedelic” drug) that may occasion mystical experiences (characterized by a profound feeling of oneness or unity) during acute effects. Such experiences may have therapeutic value. Research and clinical applications of psychedelics usually include music listening during acute drug effects, based on the expectation that music will provide psychological support during the acute effects of psychedelic drugs, and may even facilitate the occurrence of mystical experiences. However, the features of music chosen to support the different phases of drug effects are not well-specified. As a result, there is currently neither real guidance for the selection of music nor standardization of the music used to support clinical trials with psychedelic drugs across various research groups or therapists. A description of the features of music found to be supportive of mystical experience will allow for the standardization and optimization of the delivery of psychedelic drugs in both research trials and therapeutic contexts. To this end, we conducted an anonymous survey of individuals with extensive experience administering psilocybin or psilocybin-containing mushrooms under research or therapeutic conditions, in order to identify the features of commonly used musical selections that have been found by therapists and research staff to be supportive of mystical experiences within a psilocybin session. Ten respondents yielded 24 unique recommendations of musical stimuli supportive of peak effects with psilocybin, and 24 unique recommendations of musical stimuli supportive of the period leading up to a peak experience. Qualitative analysis (expert rating of musical and music-theoretic features of the recommended stimuli) and quantitative analysis (using signal processing and music-information retrieval methods) of 22 of these stimuli yielded a description of peak period music that was characterized by regular, predictable, formulaic phrase structure and orchestration, a feeling of continuous movement and forward motion that slowly builds over time, and lower perceptual brightness when compared to pre peak music. These results provide a description of music that may be optimally supportive of peak psychedelic experiences. This description can be used to guide the selection and composition of music for future psychedelic research and therapy sessions.
INCREASING WOMEN SENIOR MANAGERS EFFECTIVENESS: EVIDENCE FROM ROMANIA

In the wider context of increased participation of women to managerial positions, the issue of effectiveness at work has become equally important for female managers. Therefore it is important to identify the factors, which can determine an increase in their managerial effectiveness. This article looks at the factors conducive to managerial effectiveness in organizations, as perceived by women senior managers in Romania. The research was carried out through questionnaires and semi-structured interviews applied to a sample group of 35 Romanian women managers at various levels, in both public and private institutions. The empirical results were then analysed with theoretic instruments. The main conclusion of the research was that, for Romanian female managers, work effectiveness is determined primarily by the managerial skills and knowledge acquired by women. These in turn are connected with the years of accumulated work experience, seniority, and education, especially if it is centred on managerial education. * Dr. Farhad Analoui is a Reader in International Human Resource Management, at the Centre for International Development, Bradford University. He currently acts as associate advisor to British Council and DfJD, Director of the Professional Development Services and chairs the research cluster in management.
Multi-Target Classification Based Automatic Virtual Resource Allocation Scheme

In this paper, we propose a method for automatic virtual resource allocation by using a multi-target classification-based scheme (MTCAS). In our method, an Infrastructure Provider (InP) bundles its CPU, memory, storage, and bandwidth resources as Network Elements (NEs) and categorizes them into several types in accordance to their function, capabilities, location, energy consumption, price, etc. MTCAS is used by the InP to optimally allocate a set of NEs to a Virtual Network Operator (VNO). Such NEs will be subject to some constraints, such as the avoidance of resource over-allocation and the satisfaction of multiple Quality of Service (QoS) metrics. In order to achieve a comparable or higher prediction accuracy by using less training time than the available ensemble-based multi-target classification (MTC) algorithms, we propose a majority-voting based ensemble algorithm (MVEN) for MTCAS. We numerically evaluate the performance of MTCAS by using the MVEN and available MTC algorithms with synthetic training datasets. The results indicate that the MVEN algorithm requires 70% less training time but achieves the same accuracy as the related ensemble based MTC algorithms. The results also demonstrate that increasing the amount of training data increases the efficacy of MTCAS, thus reducing CPU and memory allocation by about 33% and 51%, respectively. key words: multi-target classification, virtual resource allocation scheme, multiple QoS
Temporal Trends in the Use of Antiplatelet Therapy in Patients With Acute Coronary Syndromes

Background: Current clinical guidelines of acute coronary syndromes (ACS) recommend the use of potent antiplatelet therapy, prasugrel or ticagrelor, because both drugs consistently reduce cardiovascular events. Purpose: The aim of this study was to examine temporal changes in the use of optimal antiplatelet therapy in patients with ACS. Methods: A total of 1717 consecutive patients admitted for ACS in 3 tertiary hospitals from February 2014 to December 2015 were enrolled. We divided these 23 months into 4 semesters: period I (0-5 months), period II (6-11 months), period III (12-17 months), and period IV (17-23 months). Demographic, clinical, and treatment data were collected both at admission and at discharge. Results: Treatment with clopidogrel remained constant throughout the periods (52%, 50%, 44%, and 50% for periods I, II, III, and IV, respectively), whereas a progressive increase in ticagrelor treatment was observed (15%, 25%, 26%, and 28%; P = .001). Indeed, new P2Y12 agents showed an increase from 47% at the first semester to 65% in patients with ST-segment elevation myocardial infarction (STEMI), and in patients younger than 75 years from 36% to 53%. However, for patients older than 75 years, diabetic, and patients with end-stage kidney disease, clopidogrel was the second most commonly used antiplatelet agent. Conclusion: In this real-life registry of patients with ACS, we observed there is still a high rate of use of clopidogrel, despite guidelines recommendations, and our analyses also showed a trend toward the use of ticagrelor. Patients who received new antiplatelet agents were patients with STEMI, younger than 75 years, and with less comorbidities. However, the use of ticagrelor and prasugrel remains low, highlighting a therapeutic inertia with considerable gap between evidence-based clinical guidelines and daily clinical practice.
Effect of the vaccines for children program on inner-city neighborhood physicians.

OBJECTIVE To determine the probable effect of the Vaccines for Children (VFC) program on immunization coverage. DESIGN Preintervention and postintervention study design, with data collected before and after enrollment in the VFC program. SETTING Twenty-three inner-city neighborhood physicians' offices in New York City. PARTICIPANTS In 1993, 30 physicians were randomly selected from 8 neighborhoods with the highest proportions of Medicaid-eligible individuals in New York City. In 1995-1996, the 30 physicians were contacted again. Twenty-three agreed to an interview and medical record review. Within each office, the medical records of children aged 3 to 35 months, with at least 3 visits in a 3-month or longer period, were randomly selected. Medical record reviews were conducted for 173 eligible children in 1993 and 528 in 1995-1996. INTERVENTIONS The VFC program was implemented in October 1994. The administration fee increased from $2 to $17.85; physicians received vaccines free. MAIN OUTCOME MEASURES Up-to-date status for immunizations and lead and tuberculosis screening; percentage of visits that are missed opportunities to immunize; and percentage of visits that were well-child visits. Up-to-date status, missed opportunities to immunize, and well-child visits were compared across time using chi 2 analysis, corrected for the use of cluster sampling. RESULTS Up-to-date status changed significantly before and alter enrollment in the VFC program (P < .05) for all immunizations and for lead and tuberculosis screening. For the diphtheria toxoid, tetanus toxoid, and pertussis vaccine, oral poliovirus vaccine, and measles, mumps, and rubella vaccine combined, coverage increased from 17.9% to 42.2%, up by 24.3 percentage points (P < .05). Missed opportunities to immunize did not change, but well-child visits increased from 15.0% to 21.6% (P < .05). Physicians generally attributed performance improvements to the VFC program and not to other competing hypotheses. CONCLUSIONS The VFC program seems to be responsible for an increase in immunization rates among these physicians.
Opinion mining on author's citation characteristics of scientific publications

Opinion mining of authors opinions on scientific papers in citations is an important feature of scientific publications. Opinion mining aims to determine the defiance of a topic with respect to the overall polarity of a document. The main engine that drives opinion mining is the processing of subjective information. A dataset in the form of sentence-based collection of over 785 citations were collected. After excluding neutral citations, the dataset of 234 opinion citations were analyzed for the presence of positive and negative features. It was observed that majority (91.6%) were found to be positive, the fraction of citations with negative orientation amounted to 8.4% and nearly 6% of the citations contained opinion terms of both positive and negative polarity. Logistic regression applied on training set resulted in 0.98 precision obtained on a classification model; hence the obtained regression model was applied on test set data to reveal positive and negative opinions.
Remodeling of Yeast CUP1 Chromatin Involves Activator-Dependent Repositioning of Nucleosomes over the Entire Gene and Flanking Sequences

ABSTRACT The yeast CUP1 gene is activated by the copper-dependent binding of the transcriptional activator, Ace1p. An episome containing transcriptionally active or inactiveCUP1 was purified in its native chromatin structure from yeast cells. The amount of RNA polymerase II on CUP1 in the purified episomes correlated with its transcriptional activity in vivo. Chromatin structures were examined by using the monomer extension technique to map translational positions of nucleosomes. The chromatin structure of an episome containing inactive CUP1 isolated from ace1Δ cells is organized into clusters of overlapping nucleosome positions separated by linkers. Novel nucleosome positions that include the linkers are occupied in the presence of Ace1p. Repositioning was observed over the entire CUP1 gene and its flanking regions, possibly over the entire episome. Mutation of the TATA boxes to prevent transcription did not prevent repositioning, implicating a chromatin remodeling activity recruited by Ace1p. These observations provide direct evidence in vivo for the nucleosome sliding mechanism proposed for remodeling complexes in vitro and indicate that remodeling is not restricted to the promoter but occurs over a chromatin domain including CUP1 and its flanking sequences.
Content comparison of occupation-based instruments in adult rheumatology and musculoskeletal rehabilitation based on the International Classification of Functioning, Disability and Health.

OBJECTIVE To compare the content of clinical, occupation-based instruments that are used in adult rheumatology and musculoskeletal rehabilitation in occupational therapy based on the International Classification of Functioning, Disability and Health (ICF). METHODS Clinical instruments of occupational performance and occupation in adult rehabilitation and rheumatology were identified in a literature search. All items of these instruments were linked to the ICF categories according to 10 linking rules. On the basis of the linking, the content of these instruments was compared and the relationship between the capacity and performance component explored. RESULTS The following 7 instruments were identified: the Canadian Occupational Performance Measure, the Assessment of Motor and Process Skills, the Sequential Occupational Dexterity Assessment, the Jebson Taylor Hand Function Test, the Moberg Picking Up Test, the Button Test, and the Functional Dexterity Test. The items of the 7 instruments were linked to 53 different ICF categories. Five items could not be linked to the ICF. The areas covered by the 7 occupation-based instruments differ importantly: The main focus of all 7 instruments is on the ICF component activities and participation. Body functions are covered by 2 instruments. Two instruments were linked to 1 single ICF category only. CONCLUSION Clinicians and researchers who need to select an occupation-based instrument must be aware of the areas that are covered by this instrument and the potential areas that are not covered at all.
Selective Epiphysiodesis of the Triradiate Cartilage for Treatment of Residual Experimental Acetabular Dysplasia

Background: The purpose of the study is to evaluate the effect of a selective epiphysiodesis of the ilioischial limb of the triradiate cartilage (TC) in an experimental dysplastic hip. Materials and Methods: A hip dysplasia was obtained in 23 New Zealand rabbits 3 weeks old by immobilizing the knee in extension with a K-wire, in 4 groups: (1) control group; (2) K-wire maintained until sacrifice; (3) K-wire removed 1 week later without epiphysiodesis; and (4) K-wire removed 1 week later with selective epiphysiodesis of the ilioischial limb of the TC. Radiographic evaluation was performed previous to knee immobilization, after K-wire removal and after sacrifice. Acetabular index (AI), Sharp’s angle, ACM angle, acetabular floor thickness ratio (AFTR) were measured and presence of dysplasia, subluxation, or dislocation was noted. The acetabular version, the acetabular sector angles, and the pelvis rotation were obtained using CT evaluation. A morphology score was developed and a morphometric study of both proximal femur and acetabulum was performed. Results: No differences between groups in the radiographic assessment before immobilization or K-wire removal were found. All cases in group 2 showed dysplasia, subluxation, or dislocation of the hip. The radiographic assessment of group 4, compared with group 3, showed a lower AI (P=0.027), Sharp’s angle (P=0.005), ACM angle (0.049), and AFTR (P=0.017). No differences between groups 1 and 4 regarding AI (P=0.08), Sharp’s angle (P=0.484), and AFTR (P=0.639) were obtained. The morphology score was lower in group 4 than in groups 2 and 3. Group 4 showed a deeper acetabulum than group 2 (P=0.008) and group 3 (P=0.033), while it was similar to group 1 (P=0.364). Conclusions: A selective epiphysiodesis of the ilioischial limb of the TC in a dysplastic hip normalized the radiographic measurements of the acetabulum and the acetabular deepness, and it improved the previous gross morphology of the capsule, labrum, and ligamentum teres in rabbits. Level of Evidence: Level II—therapeutic study.
Shadow Prices of Human Capital in Agriculture. Evidence from European FADN Regions

TThe aim of this paper is to measure the shadow price of human capital in EU agriculture and to determine whether the CAP has affected the productivity of this growth-enhancing factor. For this purpose, we used the balance sheet data for the period 1986-2012, referring to the Standard Results of the EU Farm Accountancy Data Network (FADN) farm, which is representative of commercial agriculture at regional level. Data concerning output and input price indices and education attainment levels were obtained from Eurostat and from national FADNs. DEA-VRS input-oriented annual frontiers were computed to estimate the shadow price of three levels of human capital: low, medium and high. The results show an increasing trend in the shadow prices of human capital and suggest that the shadow price of the high level of human capital has been significantly greater than the shadow price of the medium level of human capital since 1990.
An analysis and experimental approach to teaching data prefetching on CMP

At our university, since the Spring of 2005, we have been teaching a first course about data cache on CMP from computer architecture. We have accomplished several goals. The most important of which is the analysis and experimental approach for pull-based data prefetching and push-based data prefetching on CMP. The pedagogical style embodied in this course fosters a good understanding of the relationship between memory wall and data prefetching for the students early in their experience.
[Development of respiratory function investigation in Poland - historical review].

Spirometria jest uwazana za podstawowe badanie sprawności wentylacyjnej pluc. Spirometr wodny (dzwonowy) zostal skonstruowany w pierwszej polowie XIX wieku przez Hutchinsona [1]. Ten spirometr umozliwial pomiary pojemności zyciowej pluc ulegającej zmniejszeniu w roznych chorobach ukladu oddechowego i byl powszechnie uzywany przez dlugi czas. Nastepnie stosowano suchy spirometr Barnesa. W latach piecdziesiątych XX wieku pojawily sie spirografy szwedzkiej firmy Kifa, wyposazone w kimograf o zmiennej szybkości obrotow (ryc. 1). Pozwolilo to na jednoczesną rejestracje pojemności zyciowej pluc i jej skladowych, jak i po raz pierwszy na zapis natezonej objetości wydechowej pierwszosekundowej (FEV1). Oprocz tego wykorzystywano aparaty Krogha do badania przemiany podstawowej, to znaczy minutowego zuzycia tlenu w spoczynku. Zastosowanie bronchospirometru firmy Lundia, a nastepnie Pulmotestu firmy Godart umozliwilo oddzielną ocene kazdego pluca po zalozeniu dwudroznej rurki Carlensa do tchawicy. Badanie takie pozwalalo na pomiar odsetkowego udzialu w pojemności zyciowej, wentylacji minutowej i minutowego zuzycia tlenu prawego i lewego pluca. Wskazywalo to na wielkośc przeplywu krwi przez uklad naczyniowy kazdego pluca oddzielnie, co bylo pomocne w ocenie przedoperacyjnej. W Polsce pomiary spirometryczne rozpowszechnily sie w latach piecdziesiątych i sześcdziesiątych XX wieku. W Instytucie Gruźlicy i Chorob Pluc (IGiChP) w Warszawie, zwanym wowczas Instytutem Przeciwgruźliczym, z inicjatywy dyrektor prof. Janiny Misiewicz powstala pierwsza pracownia spirometryczna, utworzona w 1951 roku przez dr. A. Koziorowskiego. Kierownik tej pracowni opisal nastepnie metody badan czynnościowych narządu oddechowego i wprowadzil polskie mianownictwo [2]. Kolejne pracownie spirometryczne powstaly w ośrodkach akademickich w: Warszawie (W. Droszcz, Z. Ajewski, M. Madalinska, K. Orlowska), Sosnowcu (K. Marek [3, 4 ]), Zabrzu (J. Pudelski, K. Oklek), Krakowie (E. Nikodemowicz [5]), Wroclawiu (T. Garbinski, D. Randowa), Łodzi (S. Kuczborski, K. Gondorowicz, B. Petrykowska) i Lublinie (J. Durda, J. Hanzlik). W Wojskowym Instytu-
FACTORS AFFECTING CURRENT ACCOUNT BALANCE OF TURKEY: A SURVEY WITH THE COINTEGRATING REGRESSION ANALYSIS

In Turkish economy, especially in recent years, ratio of current account deficits to national income is considerably high. Interrelations between current account balance and other macroeconomic indicators make it important to investigate which factors to what extent affect the current account balance in terms of economic policy decisions. In this study, the relationships between current account balance and various macroeconomic variables in Turkish economy are investigated empirically with Johansen cointegration test and with cointegrating regression analyses (FMOLS, CCR and DOLS) for the 1994-2014 period. It is found that foreign trade balance is a strong explanatory of the current account balance; terms of trade and gross domestic product also have statistically significant effects on the current balance. According to the results Harberger-Laursen-Metzler (HLM) hypothesis seems to be valid for Turkey. Besides, results of the study reveal that domestic interest rates and real effective exchange rate affects Turkey’s current account balance as expected but these variables are found to be insignificant.
Community-based conservation in a globalized world

Communities have an important role to play in biodiversity conservation. However, community-based conservation as a panacea, like government-based conservation as a panacea, ignores the necessity of managing commons at multiple levels, with vertical and horizontal interplay among institutions. The study of conservation in a multilevel world can serve to inform an interdisciplinary science of conservation, consistent with the Convention on Biological Diversity, to establish partnerships and link biological conservation objectives with local development objectives. Improving the integration of conservation and development requires rethinking conservation by using a complexity perspective and the ability to deal with multiple objectives, use of partnerships and deliberative processes, and learning from commons research to develop diagnostic tools. Perceived this way, community-based conservation has a role to play in a broad pluralistic approach to biodiversity protection: it is governance that starts from the ground up and involves networks and linkages across various levels of organization. The shift of attention to processes at multiple levels fundamentally alters the way in which the governance of conservation development may be conceived and developed, using diagnostics within a pluralistic framework rather than a blueprint approach.