Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of MicroRNAs on Fucosyltransferase 8 (FUT8) Expression in Hepatocarcinoma Cells

Fucosyltransferase 8 (FUT8) catalyzes the transfer of α1,6-linked fucose to the first N-acetylglucosamine in N-linked glycans (core fucosylation). Increased core fucosylation has been reported during hepatocarcinogenesis, in both cell-associated and secreted proteins. Accordingly, increased core fucosylation of α-fetoprotein and α1-antitrypsin is currently used as a diagnostic and prognostic indicator. The present study provides new evidences that FUT8 can be regulated also through miRNA-mediated mechanisms. Using microRNA/target prediction programs, we identified miR-122 and miR-34a seed regions in the 3′ untranslated region (3′UTR) of FUT8. Then we used human and rodents hepatocarcinoma cell lines to evaluate the impact of transfection of miR-122 and miR-34a mimics on FUT8 mRNA and protein levels. This study demonstrated that forced expression of these miRNAs is able to induce a decrease of FUT8 levels and also to affect core fucosylation of secreted proteins. The ability of miR-122 and miR-34a to specifically interact with and regulate the 3′UTR of FUT8 was demonstrated via a luciferase reporter assay. Since miR-122 and miR-34a downregulation is a common feature in spontaneous human hepatocarcinoma, our finding that these miRNAs are able to target FUT8 3′UTR suggests that, together with transcriptional and other post-transcriptional systems, a miRNA-mediated mechanism could also be involved in the increased core fucosylation observed in liver tumors. Moreover, these findings also point out that miRNAs may be widely involved in the regulation of glycosylation machinery.
Toward Understanding Enterprise Architecture Management's Role in Strategic Change: Antecedents, Processes, Outcomes

As organizations face accelerated economic dynamics, it is increasingly important to improve the capability of reacting agile to changes in the marketplace. This requires implementing and adapting internal structures in a timely manner and ensuring business-IT coordination throughout the process. Enterprise architecture management (EAM) is frequently proposed as a mean to arrive at organizational forms that allow for timely reconfiguration and to guide strategy-aligned change. This explorative study seeks to contribute to an overall understanding of EAM’s application in strategic change processes. It is based on an in-depth content analysis of existing research in the field. Specifically, it identifies common EAM practices that have been suggested for application throughout the planning and implementation of strategic change. Furthermore, it reveals antecedents and outcomes of this application. The article discusses these findings in detail and summarizes the results in a preliminary process model of applying EAM for agile strategic change. contingencies.
The Level of Spoken Vocabulary Used by University English Professors and Its Effect on Students

It seems that the vocabulary knowledge of Iranian university students for English translation is below minimal comprehension. One possible factor affecting the vocabulary knowledge of students is the oral input presented by professors in their classes. This research seeks to analyze the role of oral input in vocabulary learning. For this reason, twenty- three content courses from B.A. English translation syllabus were selected. Hence, the professors` lectures were recorded and then transcribed. Next the data was analyzed with Range 32, the software developed by Paul Nation. The findings revealed that almost ninety percent of the oral vocabulary items used by professors were in the first 1000 word families that are the most frequent words in English. Moreover, the results showed that the level of vocabulary items used by professors did not change over the past semesters. Therefore, it is clear that one of the possible reasons for students’ inadequate vocabulary knowledge is lack of rich oral input, because students do not have sufficiently strong motivation to work harder to comprehend their instructors. The results of this research bring into focus the need for more systematic preparation and presentation of the vocabulary items in the course syllabus.
Introduction to object-oriented design: a minimalist approach

The tutorial takes a minimalist approach to introducing object-oriented design (OOD): Students begin working on design projects immediately, creating object models of user task scenarios, and elaborating these models throughout the day to develop a complete design. The students learn methods of responsibility-driven design, as well as how to generalize from a single problem to support class and framework reuse.
Detection of Copy-Move Forgery based on one improved LLE method

digital image forensic is an emerging frontier field of image processing area. There are many kind of forgeries, in which copy-move forgery is the most important and popular one. Then, digital image forensic which aims at detecting copy-move forgery is very important. A technique based on PCA to detect the Copy-Move Forgery has been carried out. But PCA technique can't detect the fused edge. We present a new approach based on one improved LLE. This new approach can detect not only copy-move areas but also the fused edges.
Efeito do treinamento com pesos na apatia, funções cognitivas frontais e funcionalidade motora em pacientes com doença de Alzheimer

The objectives of this study were: a) characterize the presence of apathy in community dwelling patients with AD; b) analyze the effects of resistance training on apathy, frontal cognitive functions, motor function and metabolic variables of these patients; c) verify possible relations before and after four months of resistance training in apathy, frontal cognitive functions, motor function; and d) verify if there are differences in cognitive and motor function according to the level of apathy of the patients. To do so, participated in the study 28 patients with AD, mean age 78.8 ± 6.6 years, education 4.8 ± 3.5 years who were divided into training group (TG) and group of social interaction (GSI ). All patients were evaluated according to the following tests: Clinical Dementia Rating; Modified Baecke Questionnaire for Elderly; Mini Mental State Examination; Montreal Cognitive Assessment; Semantic Verbal FluencyTest; Clock Drawing Test; Battery Frontal Assessment; Cornell Scale for Depression in dementia; Neuropsychiatric Inventory (domain Apathy); Motor Battery Test of Andreotti O Wells protocol; upper limb strength from AAHPERD battery; Sit and Stand up from a chair in 30 seconds. Besides that, the patients undertook laboratory tests to measure serum levels of total cholesterol and fractions, homocysteine and glucose. The TG participated in a resistance training protocol (RT) for four months, three times a week on nonconsecutive days lasting 60 minutes each session. The training consisted in performing three sets of 20 repetitions, with two minutes between sets and, exercises for major muscle groups. GSI participated in reading, clippings, group dynamics activities and non-systematized mild walking during the same period, frequency and duration of the RT. It was used as statistical analysis ANOVA two way, U Mann Whitney and Wilcoxon tests, and Pearson and Spearman for correlation coefficients, after verifying the data distribution by the Shapiro Wilk test. It was assumed significance level of 5% for all analysis. From the 28 participants, 12 (42.8%) presented apathy symptoms in low level. After the intervention period, the RT was effective on improving upper limb strength, flexibility, reducing serum levels of blood glucose and total cholesterol from the TG. The TP also was effective maintaining the variables: caregiver's report in relation to patients’ activities of daily living and serum levels of homocysteine. There were no reasonable relation to the objective of the study. Analyzing the patients according to the level of apathy, the apathetic group had a significant improvement in the symptoms of apathy, verbal fluency test and stand up from the ground when compared to the nonapathetic group, after experimental period.
Maestri e maestre in Italia dalla fine dell’Antico Regime alla salita al potere del Fascismo. Nascita e sviluppo di una professione.

The article examines the slow process of professionalization of elementary school teachers, from the fragmented educational reality of the old regime to the emergence of a teaching body with a specific preparation and a collective identity. The Italian State played a significant role in this process because it concerned itself with the requalification of teachers and the education of new students up to the demanding task of fighting the high levels of illiteracy and promoting people’s adherence to the new values on which it was based. Teaching, however, remained poorly paid and quite precarious and those aspects increased feminine recruitment: the job was considered a respectable and typically womanly occupation, even though the arrival of single girls in small provincial towns sometimes resulted into persecutions that found an echo in press and literature. The improvement of teachers’ cultural level made untenable the contradiction between the rhetoric exaltation of the role of the teacher and the real conditions of the profession, which also contributed to the pedagogical press. In the new century, an increasingly combative association movement developed which obtained important results, but was undermined by deep internal divisions, between men and women, urban and rural teachers, catholic and secular teachers. Teachers were accused of defending corporative interests and neglecting the school system needs, and then called to order by the Gentile’s education reform, one of the first acts of Fascist rule.
Ovarian cancer gene therapy: repeated treatment with thymidine kinase in an adenovirus vector and ganciclovir improves survival in a novel immunocompetent murine model.

OBJECTIVE Our purpose was to assess the effect of multiple injections of the system of herpes simplex virus thymidine kinase in an adenovirus vector and ganciclovir on survival in a murine model of human epithelial ovarian cancer. STUDY DESIGN In this work we tested the ability of the system of thymidine kinase delivered by an adenovirus vector and ganciclovir to treat ovarian cancer in a novel murine model for epithelial ovarian cancer, SaskMouse. SaskMouse was developed by injecting LM-1 cells, a murine epithelial ovarian cancer cell line, intraperitoneally into a syngeneic C57BL/6N x C3H/He mouse strain. The cells developed into multiple cancer implants on different abdominal organs, leading to ascites and rapid death. The model has an intact immune system, as evidenced by the inability of different human cancer cells to develop into cancers when injected into the mice intraperitoneally. RESULTS The system of thymidine kinase delivered by an adenovirus vector and ganciclovir was applied to SaskMouse. Mice were either untreated (group 1), treated with one intraperitoneal injection of adenovirus- thymidine kinase at 250 plaque-forming units/cell (group 2), or treated with two intraperitoneal injections of adenovirus-thymidine kinase at 250 plaque-forming units/cell on days 0 and 23 (group 3). Survivals were 23 +/- 2, 27 +/- 2, and 35 +/- 4 days, respectively (P <.05). Antiadenoviral antibodies were assayed both in the serum and in the peritoneal fluid of treated mice. Despite high antibody titers in serum, there were no detectable antibodies in the peritoneal fluid. CONCLUSION Our data suggest that multiple intraperitoneal injections of the combination of thymidine kinase delivered by an adenovirus vector and ganciclovir are effective in prolonging survival in the presence of ovarian cancer. There are potential implications for other abdominal malignancies.
RDFChain: Chain Centric Storage for Scalable Join Processing of RDF Graphs using MapReduce and HBase

As a massive linked open data is available in RDF, the scalable storage and efficient retrieval using MapReduce have been actively studied. Most of previous researches focus on reducing the number of MapReduce jobs for processing join operations in SPARQL queries. However, the cost of shuffle phase still occurs due to their reduce-side joins. In this paper, we propose RDFChain which supports the scalable storage and efficient retrieval of a large volume of RDF data using a combination of MapReduce and HBase which is NoSQL storage system. Since the proposed storage schema of RDFChain reflects all the possible join patterns of queries, it provides a reduced number of storage accesses depending on the join pattern of a query. In addition, the proposed cost-based map-side join of RDFChain reduces the number of map jobs since it processes as many joins as possible in a map job using statistics.
Combining Psychotherapy and Medication for College Students with Severe Psychopathology

Abstract Given the extensive and increasing use of medications to augment psychotherapy in intervening with college students with more severe psychopathology, the absence of scholarship on this topic is surprising. This article briefly summarizes earlier published pieces on combining counseling with psychotropic treatment in college counseling center settings. It then reports the results of a retrospective, descriptive study of 87 college counseling center clients receiving a combination of psychotherapy and psychiatric medication intervention. Pre-post change psychosocial impairment scores on the Carroll Rating Scale (CRS) were employed as the outcome measure. Most, but not all clients showed significant improvement on this index.
Creating and curing fatty hearts

Purpose of reviewDiseases associated with ectopic disposition of lipids are becoming an increasingly important medical problem as the incidence of type 2 diabetes and obesity increases. One of the organs affected by lipotoxicity is the heart and this review presents an update on human and animal studies of this problem. Recent findingsHuman studies have clearly correlated heart dysfunction with the content of triglyceride. More recently human heart samples have been used to assess gene changes associated with altered lipid accumulation. Genetically altered mice have been created that develop lipotoxic cardiomyopathies and newer investigations are attempting to delineate curative therapies. SummaryHuman studies will confirm the metabolic changes associated with lipotoxic cardiomyopathy and, hopefully, animal studies will guide treatment options.
Minimizing sparse higher order energy functions of discrete variables

Higher order energy functions have the ability to encode high level structural dependencies between pixels, which have been shown to be extremely powerful for image labeling problems. Their use, however, is severely hampered in practice by the intractable complexity of representing and minimizing such functions. We observed that higher order functions encountered in computer vision are very often “sparse”, i.e. many labelings of a higher order clique are equally unlikely and hence have the same high cost. In this paper, we address the problem of minimizing such sparse higher order energy functions. Our method works by transforming the problem into an equivalent quadratic function minimization problem. The resulting quadratic function can be minimized using popular message passing or graph cut based algorithms for MAP inference. Although this is primarily a theoretical paper, it also shows how higher order functions can be used to obtain impressive results for the binary texture restoration problem.
Network-Aware HEFT Scheduling for Grid

We present a network-aware HEFT. The original HEFT does not take care of parallel network flows while designing its schedule for a computational environment where computing nodes are physically at distant locations. In the proposed mechanism, such data transfers are stretched to their realistic completion time. A HEFT schedule with stretched data transfers exhibits the realistic makespan of the schedule. It is shown how misleading a schedule can be if the impact of parallel data transfers that share a bottleneck is ignored. A network-aware HEFT can be used to yield a benefit for Grid applications.
Phosphatidylinositol 4,5-Bisphosphate Influences PIN Polarization by Controlling Clathrin-Mediated Membrane Trafficking in Arabidopsis[C][W]

Plant growth follows positional cues provided by the phytohormone auxin. A key determinant of auxin distribution is the asymmetric plasma membrane localization of PIN-auxin transporters, which involves complex endocytotic cycling. Endocytosis and PIN distribution require the regulatory phospholipid, PtdIns(4,5)P2, which is formed by PI4P 5-kinases that themselves display polarized distribution. The functions of the minor phospholipid phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] during vegetative plant growth remain obscure. Here, we targeted two related phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) PIP5K1 and PIP5K2, which are expressed ubiquitously in Arabidopsis thaliana. A pip5k1 pip5k2 double mutant with reduced PtdIns(4,5)P2 levels showed dwarf stature and phenotypes suggesting defects in auxin distribution. The roots of the pip5k1 pip5k2 double mutant had normal auxin levels but reduced auxin transport and altered distribution. Fluorescence-tagged auxin efflux carriers PIN-FORMED (PIN1)–green fluorescent protein (GFP) and PIN2-GFP displayed abnormal, partially apolar distribution. Furthermore, fewer brefeldin A–induced endosomal bodies decorated by PIN1-GFP or PIN2-GFP formed in pip5k1 pip5k2 mutants. Inducible overexpressor lines for PIP5K1 or PIP5K2 also exhibited phenotypes indicating misregulation of auxin-dependent processes, and immunolocalization showed reduced membrane association of PIN1 and PIN2. PIN cycling and polarization require clathrin-mediated endocytosis and labeled clathrin light chain also displayed altered localization patterns in the pip5k1 pip5k2 double mutant, consistent with a role for PtdIns(4,5)P2 in the regulation of clathrin-mediated endocytosis. Further biochemical tests on subcellular fractions enriched for clathrin-coated vesicles (CCVs) indicated that pip5k1 and pip5k2 mutants have reduced CCV-associated PI4P 5-kinase activity. Together, the data indicate an important role for PtdIns(4,5)P2 in the control of clathrin dynamics and in auxin distribution in Arabidopsis.
iLight: Indoor device-free passive tracking using wireless sensor networks

Target tracking is a main application of wireless sensor networks (WSNs), and has been studied widely [4], [10]. In this work, we study indoor passive tracking problem using WSNs, in which we assume no equipment is carried by the target and the tracking procedure is passive. We propose to use light to track a moving target in WSNs. To our best knowledge, this is the first work which tracks a moving object by using light sensors and general light sources. We design a novel probabilistic protocol (system) iLight to track a moving target and several efficient methods to compute the target's moving patterns (like height, etc.) at the same time. We implement and evaluate our tracking system iLight in a testbed consisting of 40 sensor nodes, 10 general light sources and one base station. Through extensive experiments, we show that iLight can track a moving target efficiently and accurately.
Hospital Variation in Functional Recovery After Stroke

Background— Functional status is a key patient-centric outcome, but there are little data on whether functional recovery post-stroke varies among hospitals. This study examined the distribution of functional status 3 months after stroke, determined whether these outcomes vary among hospitals, and identified hospital characteristics associated with better (or worse) functional outcomes. Methods and Results— Observational analysis of the AVAIL study (Adherence Evaluation After Ischemic Stroke-Longitudinal) included 2083 ischemic stroke patients enrolled from 82 US hospitals participating in Get With The Guidelines-Stroke and AVAIL. The primary outcome was dependence or death at 3 months (modified Rankin Scale [mRS] score of 3–6). Secondary outcomes included functional dependence (mRS score of 3–5), disabled (mRS score of 2–5), and mRS evaluated as a continuous score. By 3 months post-discharge, 36.5% of patients were functionally dependent or dead. Rates of dependence or death varied widely by discharging hospitals (range: 0%–67%). After risk adjustment, patients had lower rates of 3-month dependence or death when treated at teaching hospitals (odds ratio, 0.72; 95% confidence interval, 0.54–0.96) and certified primary stroke centers (odds ratio, 0.69; 95% confidence interval, 0.53–0.91). In contrast, a composite measure of hospital-level adherence to acute stroke care performance metrics, stroke volume, and bed size was not associated with downstream patient functional status. Findings were robust across mRS end points and sensitivity analyses. Conclusions— One third of acute ischemic stroke patients were functionally dependent or dead 3 months postacute stroke; functional recovery rates varied considerably among hospitals, supporting the need to better determine which care processes can maximize functional outcomes.
Mapping the Binding Site of a Large Set of Quinazoline Type EGF-R Inhibitors Using Molecular Field Analyses and Molecular Docking Studies

In the current work, three-dimensional QSAR studies for one large set of quinazoline type epidermal growth factor receptor (EGF-R) inhibitors were conducted using two types of molecular field analysis techniques: comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA). These compounds belonging to six different structural classes were randomly divided into a training set of 122 compounds and a test set of 13 compounds. The statistical results showed that the 3D-QSAR models derived from CoMFA were superior to those generated from CoMSIA. The most optimal CoMFA model after region focusing bears significant cross-validated r(2)(cv) of 0.60 and conventional r(2) of 0.92. The predictive power of the best CoMFA model was further validated by the accurate estimation to these compounds in the external test set, and the mean agreement of experimental and predicted log(IC(50)) values of the inhibitors is 0.6 log unit. Separate CoMFA models were conducted to evaluate the influence of different partial charges (Gasteiger-Marsili, Gasteiger-Hückel, MMFF94, ESP-AM1, and MPA-AM1) on the statistical quality of the models. The resulting CoMFA field map provides information on the geometry of the binding site cavity and the relative weights of various properties in different site pockets for each of the substrates considered. Moreover, in the current work, we applied MD simulations combined with MM/PBSA (Molecular mechanics/Possion-Boltzmann Surface Area) to determine the correct binding mode of the best inhibitor for which no ligand-protein crystal structure was present. To proceed, we define the following procedure: three hundred picosecond molecular dynamics simulations were first performed for the four binding modes suggested by DOCK 4.0 and manual docking, and then MM/PBSA was carried out for the collected snapshots. The most favorable binding mode identified by MM/PBSA has a binding free energy about 10 kcal/mol more favorable than the second best one. The most favorable binding mode identified by MM/PBSA can give satisfactory explanation of the SAR data of the studied molecules and is in good agreement with the contour maps of CoMFA. The most favorable binding mode suggests that with the quinazoline-based inhibitor, the N3 atom is hydrogen-bonded to a water molecule which, in turn, interacts with Thr 766, not Thr 830 as proposed by Wissner et al. (J. Med. Chem. 2000, 43, 3244). The predicted complex structure of quinazoline type inhibitor with EGF-R as well as the pharmacophore mapping from CoMFA can interpret the structure activities of the inhibitors well and afford us important information for structure-based drug design.