Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain

Porcine epidemic diarrhea virus (PEDV), which re-emerged in China in 2010, has caused severe economic losses to the global pig industry. In this study, a PEDV strain, designated PEDV WMB, was isolated from piglets with severe diarrhea on a pig farm in Henan Province of China. Whole-genome sequencing and analysis revealed that the PEDV WMB strain belongs to subtype G2c and has a unique continuous mutation in the S10 antigenic epitope of the S protein. Moreover, the virus-neutralization (VN) test indicated that polyclonal antibodies against the S10 protein of other G1 and G2 strains showed reduced VN reactivity to PEDV WMB. The pathogenicity of PEDV WMB was further investigated in 3 day-old piglets. PEDV infection-related clinical symptoms and morphological lesions were observed and confirmed by histopathological and immunohistochemical examination (IHC). These results illustrated that continuous mutation of the S10 epitope might affect the immunogenicity or pathogenicity of PEDV, providing evidence of the need to monitor the genetic diversity of the virus and develop effective measures to prevent and control PEDV.
Correlation of systemic immune-inflammation index and moderate/major depression in patients with depressive disorders: a large sample cross-sectional study

Objective To evaluate the clinical value of systemic immune-inflammation index (SII) based on peripheral blood neutrophil, lymphocyte, and platelet count in evaluating the subtype and severity of depression in patients with depressive disorder. Methods This retrospective cohort study was conducted in the Third People’s Hospital of Fuyang City from January 1, 2020 to December 31, 2022. The data included sociodemographic information at admission, clinical data, discharge diagnosis and inflammatory markers. Patients were divided into low SII group and high SII group according to the optimal threshold of SII determined by receiver operating characteristic curve (ROC curve). Binary logistic regression was used to analyze the correlation between moderate/major depression and SII level. Results Compared to the low SII group, the high SII group had a higher age level (χ2 = 7.663, p = 0.006), more smokers (χ2 = 9.458, p = 0.002), more moderate/major depression patients (χ2 = 45.645, p < 0.001), and a higher proportion of patients with accompanying somatic symptoms (χ2 = 14.867, p < 0.001). In the final logistic regression model, after controlling for confounding factors, SII at admission was significantly associated with moderate/major depression [β =1.285, p < 0.001; odds ratio (95% confidence intervals) = 3.614 (2.693–4.850)]. Patients with high SII scores were 3.614 times more likely to have moderate/severe depression than those with low SII scores. We propose a cut-off value of SII =540.78 (sensitivity = 36.4% and specificity = 80.3%) according to the maximum Youden index. Conclusion Our research indicates that SII may be a useful, repeatable, convenient, and affordable index to identify moderate/major depression in depressive disorder.
Optimization of embryonic thermal programming confirms increased liver fattening in mule ducks and changes in lipid metabolism

Introduction: The embryonic thermal programming (TM) in birds has been shown to impact several physiological parameters such as resistance to thermal stress, muscle growth or immunity. In mule ducks, it has recently been shown that TM can induce metabolic programming resulting in increased liver weight and fat storage after overfeeding. However, a decrease in hatchability and foie gras quality was also observed, suggesting that this technique needs to be optimized. Here, we tested a new thermal manipulation condition determined with the objective of avoiding negative impacts while maintaining or improving liver properties. Methods: The eggs of the control group were incubated at 37.6°C during the whole incubation period while those of the experimental group (TM group) were incubated at 39.3°C 16 h/24 h from the 11th day of incubation to the 21st. After hatching, all the animals were fed and raised under the same conditions until the age of 12 weeks. At this stage, one part of the animals was overfed and then slaughtered 2 h (to measure rapid changes in metabolism) or 10 h after the last meal (to obtain the best technological yields), while the other part was ration-fed and slaughtered 2 h after the last meal, at the same age. Results: An 8% increase in foie gras production was measured in the TM group compared to the control group without altering the quality of the final product (nor hatchability), confirming the successful optimization of the metabolic programming. Interestingly, these results allowed us not to reject the previously suggested hypothesis of a potential delay in metabolic processes involved in liver fattening in programmed animals, in particular by measuring a trend reversal regarding the amount of total hepatic lipids in both groups at 2 h and then 10 h after the last meal. Discussion: This study therefore validates the optimization of metabolic programming by embryonic thermal manipulation for duck liver fattening. The understanding of the mechanisms of embryonic thermal programming in birds remains today very incomplete and the search for epigenetic marks (main hypothesis of the concept of programming) at the origin of the observed phenotypes could be the next step of this work.
High-frequency rTMS over the left DLPFC improves the response inhibition control of young healthy participants: an ERP combined 1H-MRS study

Introduction Unlike the effect of repetitive transcranial magnetic stimulation (rTMS) in treating neuropsychiatric diseases, little is known about how personal factors might account for the disparity of results from studies of cognition and rTMS. In this study, we investigated the effects of high-frequency rTMS on response inhibition control and explored the time course changes in cognitive processing and brain metabolic mechanisms after rTMS using event-related potentials (ERPs) and magnetic resonance spectroscopy (1H-MRS). Methods Participants were all right-handed and were naive to rTMS and the Go/NoGo task. Twenty-five healthy young participants underwent one 10 Hz rTMS session per day in which stimulation was applied over the left dorsolateral prefrontal cortex (DLPFC), and a homogeneous participant group of 25 individuals received a sham rTMS treatment for 1 week. A Go/NoGo task was performed, an electroencephalogram (EEG) was recorded, and 1H-MRS was performed. Results The results revealed that there was a strong trend of decreasing commission errors of NoGo stimuli by high frequency rTMS over the left DLPFC, whereas there was no significant difference between before and after rTMS treatment with respect to these parameters in the sham rTMS group. High-frequency rTMS significantly increased the amplitude of NoGo-N2 but not Go-N2, Go-P3, or NoGo-P3. The myo-inositol /creatine complex (MI/Cr) ratio, indexing cerebral metabolism, in the left DLPFC was decreased in the rTMS treated group. Discussion This observation supports the view that high-frequency rTMS over the left DLPFC has the strong tendency of reducing commission errors behaviorally, increase the amplitude of NoGo-N2 and improve the response inhibition control of healthy young participants. The results are consistent with the excitatory properties of high frequency rTMS. We suggest that the increase in the NoGo-N2 amplitude may be related to the increased excitability of the DLPFC-anterior cingulate cortex (ACC) neural loop. Metabolic changes in the DLPFC may be a possible mechanism for the improvement of the response inhibition control of rTMS.
Pan-cancer analysis identifies the correlations of Thymosin Beta 10 with predicting prognosis and immunotherapy response

Introduction The biological function and prognosis roles of thymosin β(TMSB) 10 are still unclear in pan-cancer. Methods We retrieved The Cancer Genome Atlas and Genotype-tissue expression datasets to obtain the difference of TMSB10 expression between pan-cancer and normal tissues, and analyzed the biological function and prognosis role of TMSB10 in pan-cancer by using cBioPortal Webtool. Results The expression of TMSB10 in tumor tissues was significantly higher than normal tissues, and showed the potential ability to predict the prognosis of patients in Pan-cancer. It was found that TMSB10 was significantly correlated with tumor microenvironment, immune cell infiltration and immune regulatory factor expression. TMSB10 is involved in the regulation of cellular signal transduction pathways in a variety of tumors, thereby mediating the occurrence of tumor cell invasion and metastasis. Finally, TMSB10 can not only effectively predict the anti-PD-L1 treatment response of cancer patients, but also be used as an important indicator to evaluate the sensitivity of chemotherapy. In vitro, low expression of TMSB10 inhibited clonogenic formation ability, invasion, and migration in glioma cells. Furthermore, TMSB10 may involve glioma immune regulation progression by promoting PD-L1 expression levels via activating STAT3 signaling pathway. Conclusions Our results show that TMSB10 is abnormally expressed in tumor tissues, which may be related to the infiltration of immune cells in the tumor microenvironment. Clinically, TMSB10 is not only an effective prognostic factor for predicting the clinical treatment outcome of cancer patients, but also a promising biomarker for predicting the effect of tumor immune checkpoint inhibitors (ICIs) and chemotherapy in some cancers.
Ralstonia solanacearum type III effector RipAA targets chloroplastic AtpB to modulate an incompatible interaction on Nicotiana benthamiana

Introduction The type III effector RipAA of Ralstonia solanacearum GMI1000 plays a critical role in the incompatible interaction on Nicotiana benthamiana. Methods The RipAA was transiently expressed in N. benthamiana by Agrobacterium-mediated transformation. Chemical staining with trypan blue and DAB were conducted to examine the cell death and the accumulation of hydrogen peroxide (H2O2), respectively. The expression of the marker genes for salicylic acid (SA) and jasmonic acid (JA) signaling was evaluated by quantitative reverse transcription PCR (qRT-PCR). The proteins interacted with RipAA was identified from N. benthamiana by yeast two-hybrid and pull-down assays. A TRV-mediated gene silencing was used to assess the role of host gene in response to RipAA expression and R. solanacearum infection. Results and discussion RipAA induced the accumulation of hydrogen peroxide (H2O2) and genome DNA degradation in N. benthamiana, which were accompanied by a hypersensitive reaction. Simultaneously, the marker genes for salicylic acid (SA) signaling were induced and those for jasmonic acid (JA) signaling were reduced. N. benthamiana chloroplastic AtpB, the ATPase β subunit, was identified as an interactor with RipAA. The silencing of atpB in N. benthamiana resulted in the inability of RipAA to induce a hypersensitive response, a compatible interaction with GMI1000, and an enhanced sensitivity to bacterial wilt. Our data support the concept that RipAA determines host-range specificity by targeting the host chloroplastic AtpB.
Effect of Environment on the Fidelity of Control and Measurements of Solid-State Quantum Devices

Abstract : This thesis addresses the origin and effect of noise and fluctuations in quantum devices that find applications in quantum information processing. In the first half of the thesis, we consider a phenomenological model of the noise and study its effect on the fidelity of quantum measurements and operations that are essential for quantum computing. We focus primarily on quantum gate operations in phase qubits, detection of microwave photons and the measurement of the Berry curvature. Specifically, we examine the effect of the Ohmic noise on optimally controlled flux-biased phase qubits for one- and two-quadrature microwave pulses and demonstrate that twoquadrature pulses with fixed driving frequency are as robust as variable driving frequency, in the presence of environment. Next, we present a model to analyze the quantum efficiency of a microwave photon detector based on a current-biased Josephson junction and study the effect of decoherence on the detection efficiency of the detector. We also present alternative set-ups for microwave photon detection and provide a systematic method to compute the power absorbed by the detector. We then consider the effect of decoherence on the Berry curvature measurement, which employs a novel non-adiabatic protocol, and show that the curvature is immune to decoherence. In the second half of the thesis, we go beyond the phenomenological models of the noises and perform a detailed study of the microscopic description of the Johnson noise. Here we focus primarily on quantum dot devices. We present a formalism to compute relaxation rates in charge and spin qubits due to evanescent wave Johnson noise (EWJN) from the metallic gates that are in proximity to quantum dots. The EWJN is analyzed for the metallic gates that are characterized by both local and nonlocal dielectric response functions.
A masked, placebo-controlled, randomized clinical trial evaluating safety and the effect on cardiac function of low-dose rapamycin in 17 healthy client-owned dogs

Introduction: Geroscience studies of low-dose rapamycin in laboratory species have identified numerous benefits, including reversing age-related cardiac dysfunction. Cardiovascular benefits have been observed in dogs with 10 weeks of treatment, raising questions about possible benefits and adverse effects of long-term use of low-dose rapamycin. The objectives of this study were to assess the impact of 6 months of low-dose rapamycin on echocardiographic indices of cardiac function in healthy dogs and to document the occurrence of adverse events. Methods: Seventeen client-owned dogs aged 6–10 years, weighing 18–36 kg, and without significant systemic disease were included in a prospective, randomized, placebo-controlled, masked clinical trial. Low-dose rapamycin (0.025 mg/kg) or placebo was administered three times per week for 6 months. Baseline, 6-month, and 12-month evaluation included physical examination, cardiology examination, and clinicopathology. Three-month evaluation included physical examination and clinicopathology. Owners completed online questionnaires every 2 weeks. Results: There were no statistically significant differences in echocardiographic parameters between rapamycin and placebo groups at 6 or 12 months. No clinically significant adverse events occurred. In 26.8% of the bi-weekly surveys owners whose dogs received rapamycin reported perceived positive changes in behavior or health, compared to 8.1% in the placebo group (p = 0.04). Discussion: While no clinically significant change in cardiac function was observed in dogs treated with low-dose rapamycin, the drug was well-tolerated with no significant adverse events.
Advances and perspectives in the development of vaccines against highly pathogenic bunyaviruses

Increased human activities around the globe and the rapid development of once rural regions have increased the probability of contact between humans and wild animals. A majority of bunyaviruses are of zoonotic origin, and outbreaks may result in the substantial loss of lives, economy contraction, and social instability. Many bunyaviruses require manipulation in the highest levels of biocontainment, such as Biosafety Level 4 (BSL-4) laboratories, and the scarcity of this resource has limited the development speed of vaccines for these pathogens. Meanwhile, new technologies have been created, and used to innovate vaccines, like the mRNA vaccine platform and bioinformatics-based antigen design. Here, we summarize current vaccine developments for three different bunyaviruses requiring work in the highest levels of biocontainment: Crimean-Congo Hemorrhagic Fever Virus (CCHFV), Rift Valley Fever Virus (RVFV), and Hantaan virus (HTNV), and provide perspectives and potential future directions that can be further explored to advance specific vaccines for humans and livestock.
Tunable plasmonics with Au nanoparticles coupled to thin film vanadium dioxide

: The development of active devices featuring dynamic tunable plasmonic resonances is vital for wide implementation in many optoelectronic devices. By coupling plasmonic nanoparticles to an underlying thin film of phase change material vanadium dioxide (VO 2 ), dynamic tuning of the plasmon resonance can be achieved within the visible and near IR spectral regions. It is shown through selection of single nanoparticle or dimer structures, the plasmon resonances can be tuned over a large spectral range, the scattering cross-section can be increased, and the E-field enhancement and spatial profile can be controlled via the VO 2 phase transition. Hybrid Au nanocuboid–VO 2 structures exhibit larger reversible wavelength shifts than rounded nanoparticles, such as rods and discs, of similar dimensions. A plasmon resonance shift of over 600 nm is observed in the near-IR after the semiconducting to metallic VO 2 phase transition. The largest increases in the scattering cross-section are achieved with a VO 2 thin film thickness of 30–50 nm. Disc, rod, bowtie and cuboid dimers show larger increases of the scattering cross-section at lower wavelengths, even extending into the visible spectral range. On VO 2 phase transition the bowtie dimers can provide an increase in the scattering cross-section of over 70% and 3.6-fold increase in the E-field intensity within the dimer gap. Additionally, the near-field enhancement spreads over the entire height of the dimer gap, and in particular, there is a large enhancement at the surface of the dimers. The increased scattering cross-section and modification of the spatial profile of the E-field enhancement provides mechanisms for tunable metasurfaces.
Role of Servant Leadership Style on Leadership Effectiveness: Moderating Role of Spirituality

Servant leadership falls in transformational leadership style. In this style leaders’ main purpose is to serve his people, customers, employees, societies and communities first. A good leader must be visionary, listen to problems of others and help them to solve it. Aim of the study is to investigate the moderating role of spirituality on relationship between servant leadership style and leadership effectiveness. In order to enhance leaders’ effectiveness servant leadership along with spirituality i.e. ethics are taken as predictors. This study is quantitative in nature. Survey approach is used. Self-administered questionnaire is used to collect the data from respondents in universities. Primary cross-sectional data is collected from the respondents from public and private sector universities. Primary data is called first hand data which is collected for the first time. Cross-sectional means data collected at point of time. Non probability snow ball sampling technique is used for collecting the sample size. SPSS is used for reliability, validity, and testing of hypotheses. Reliability is checked through Cronbach alpha. Findings revealed that scales used in the study are reliable and valid. Correlations are found significant but moderating effect is not significant. It is recommended that religious practices can enhance the effectiveness of leadership if teachers present their selves as role model to students Academicians, managers can take benefit from the findings of the study. Practitioners must raise awareness about spirituality and servant leadership style in conferences, seminars, workshops and training sessions. The originality of the study lies in investigating the moderating role of spirituality on the relationship between servant leadership and leadership effectiveness
Adaptive iterative control for random values impulse noise filtering

The random value impulse noise modeling is defined,it is more realistic noise conditions,an adaptive controlling iteration times filter algorithm is proposed for the random value impulse noise remove.Algorithm consists of four steps,noise detection,noise filtering,error detection pixel correction and iterative control.The algorithm can remove fixed-value noise and random-value impulse noise effectively,and can effectively filter out fixed value impulse noise and random value impulse noise.Compared with median value filtering algorithm,this algorithm can protect filtered image information better,and there is no need to set the iteration parameters in the filtering process,with strong adaptability.
Real-life Bifurcation - Challenges and Potential Complications

Together with calcified lesions, saphenous vein grafts, chronic total occlusions and unprotected left main lesions, bifurcation lesions are complex lesions that remain among the outstanding challenges of treatment with percutaneous coronary intervention. Bifurcation lesions are associated with increased rates of procedural complications, restenosis and adverse events than lesions in the body of the vessel. The introduction of drug-eluting stents for the treatment of bifurcation lesions has dramatically decreased restenosis rates, especially in patients suffering from diabetes. However, abrupt side branch closure, side branch ostial restenosis and stent thrombosis remain areas where further improvement is needed. Although a provisional T-stent strategy is most often used when side branch stenting is required, there are true bifurcation lesions where the selected use of more complex bifurcation approaches (such as the crush technique, T-stenting or the culotte technique) seem appropriate, particularly when the main branch and side branch are larger vessels with more diffuse side branch disease. The major challenge with any technique is to ensure that the side branch is protected and there is a satisfactory final result. Many technical questions rise in trying to ensure this outcome and lower the risk of intra- and post-procedural complications such as side branch closure and restenosis, stent thrombosis, dissection and fracture of a jailed wire: how can difficult side branch access be solved? How can unfavourable side branch anatomy be re-wired after main vessel stent placement? How can fracture of a jailed wire be avoided? Is side-strut dilation beneficial?
12. Formation, change, and contextualization of mental associations: determinants and principles of variations in implicit measures

S tatting with the development of a new class of indirect measurement procedures in the mid~ 1990s, research using these procedures has pro~ duced a plethora of remarkable findings that has stimulated wide interest far beyond the traditional boundaries of social psychology1 People who consciously endorse egalitarian values tend to be quite astonished when they learn that their responses assessed by indirect procedures show racial bias (Nosek et al., 2007; see also Amodio, & Mendoza, Chapter 19, and Trawalter & Shapiro, Chapter ZO, this volume); spontaneous behaviors that are dif~ ficult to predict with self~report measures revealed strong relations to implicit measures (Friese, Hof mann, & Schmitt, 2008; see also Perugini, Richetin, & Zogmaister, Chapter 14, this volume); and deliberate decisions with important real~life impli~ cations have been found to be predictable by re~ sponse latency differences in the range of millisec~ onds obtained by indirect procedures (e.g., Galdi, Arcuri, & Gawronski, 2008; Green et al., 2007; von Hippel, Brener, & von Hippel, 2008; see also Bodenhausen & Todd, Chapter 15, this volume). Given the ubiquity of such findings, researchers became interested in potential sources of the men~ tal associations assessed by indirect procedures, in particular the principles underlying their forma~ tion, change, and contextualization.