Miracle-dz/UniFAR-FTUs huggingface dataset:
Anaerobic expression of the gadE-mdtEF multidrug efflux operon is primarily regulated by the two-component system ArcBA through antagonizing the H-NS mediated repression

The gadE-mdtEF operon encodes a central acid resistance regulator GadE and two multidrug efflux proteins MdtEF. Although transcriptional regulation of gadE in the context of acid resistance under the aerobic growth environment of Escherichia coli has been extensively studied, regulation of the operon under the physiologically relevant environment of anaerobic growth and its effect on the expression of the multidrug efflux proteins MdtEF in the operon has not been disclosed. Our previous study revealed that anaerobic induction of the operon was dependent on ArcA, the response regulator of the ArcBA two-component system, in the M9 glucose minimal medium. However, the detailed regulatory mechanism remains unknown. In this study, we showed that anaerobic activation of mdtEF was driven by the 798 bp unusually long gadE promoter. Deletion of evgA, ydeO, rpoS, and gadX which has been shown to activate the gadE expression during acid stresses under aerobic condition did not have a significant effect on the anaerobic activation of the operon. Rather, anaerobic activation of the operon was largely dependent on the global regulator ArcA and a GTPase MnmE. Under aerobic condition, transcription of gadE was repressed by the global DNA silencer H-NS in M9 minimal medium. Interestingly, under anaerobic condition, while ΔarcA almost completely abolished transcription of gadE-mdtEF, further deletion of hns in ΔarcA mutant restored the transcription of the full-length PgadE-lacZ, and P1- and P3-lacZ fusions, suggesting an antagonistic effect of ArcA on the H-NS mediated repression. Taken together, we conclude that the anaerobic activation of the gadE-mdtEF was primarily mediated by the two-component system ArcBA through antagonizing the H-NS mediated repression.
Automated Edge Detection Using Convolutional Neural Network

The edge detection on the images is so important for image processing. It is used in a various fields of applications ranging from real-time video surveillance and traffic management to medical imaging applications. Currently, there is not a single edge detector that has both efficiency and reliability. Traditional differential filter-based algorithms have the advantage of theoretical strictness, but require excessive post-processing. Proposed CNN technique is used to realize edge detection task it takes the advantage of momentum features extraction, it can process any input image of any size with no more training required, the results are very promising when compared to both classical methods and other ANN based methods
New Rain Rate Statistics for Emerging Regions: Implications for Wireless Backhaul Planning

As demand for broadband service increases in emerging regions, high-capacity wireless links can accelerate and cost-reduce the deployment of new networks (both backhaul and customer site connection). Such links are increasingly common in developed countries, but their reliability in emerging regions is questioned where very heavy tropical rain is present. Here, we investigate the robustness of the standard (ITU-R P.837-6) method for estimating rain rates using an expanded test dataset. We illustrate how bias/variance issues cause problematic predictions at higher rain rates. We confirm (by construction) that an improved rainfall climatology can largely address these prediction issues without compromising standard ITU fit evaluation metrics.
Inhibition of α-Glucosidase by Thiosulfinate as a Target for Glucose Modulation in Diabetic Rats

Postprandial hyperglycemia is a predisposing factor for vascular dysfunction and organ damage. α-glucosidase is a hydrolytic enzyme that increases the glucose absorption rate and subsequently elevates blood glucose levels. Garlic (Allium sativum L.) is a rich source of several phytonutrients, including thiosulfinate (THIO). The aim of this study was to evaluate the ability of THIO, a potent inhibitor of intestinal α-glucosidase, to reduce postprandial blood glucose. Male albino rats were randomly assigned to five different groups (n = 10/group). Group 1 served as the control group. Groups 2–5 were injected intraperitoneally with a single dose of streptozotocin (STZ) to induce diabetes. Group 2 comprised untreated diabetic rats. Groups 3 and 4 contained diabetic rats that were given THIO orally (20 mg/kg body weight/day and 40 mg/kg body weight/day, resp.). Group 5 was the positive control having diabetic rats treated orally with acarbose (10 mg/kg body weight/day; positive control). Diabetic rats treated with THIO displayed a significant blood glucose reduction (p < 0.001 and < 0.01 by analysis of variance, resp.) and a significant elevation in insulin compared with that of untreated rats. THIO is an effective noncompetitive intestinal α-glucosidase inhibitor that promotes hypoglycemic action (p < 0.001) in STZ-injected rats. THIO is a promising agent for the management of postprandial hyperglycemia.
In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

The aim of the present study was to track in vivo the distribution and survival of adipose-derived mesenchymal stem cells (ASCs) transplanted into female BALB/c nude mice following simulated childbirth injury, using green fluorescent protein and luciferase dual labeling, bioluminescent imaging (BLI) and histological evaluation. The results demonstrated that the dually labeled ASCs could be detected for up to eight weeks in vivo. The number of implanted cells decreased during the first three weeks, and then stabilized until the end of the experiment. According to the linear regression plot, ~27,621 implanted cells survived until eight weeks after implantation. Transplanted ASCs predominantly existed at the inoculation site of the vagina, with little or no spread to other organs. Histological analysis confirmed the survival of the engrafted ASCs. The study provided basic evidence that BLI techniques can be used to monitor ASCs in vivo in real time and in the long term. Through local administration, ASCs could survive in the long term to facilitate repair following pelvic-floor injury.
Trajectory data analysis using complex networks

A massive amount of data on moving object trajectories is available today. However, it is still a major challenge to process such information in order to explain moving object interactions, which could help in revealing non-trivial behavioral patterns. To that end, we consider a complex networks-based representation of trajectory data. Frequent encounters among moving objects (trajectory encounters) are used to create the network edges whereas nodes represent trajectories. A real trajectory dataset of vehicles moving within the City of Milan allows us to study the structure of vehicle interactions and validate our method. We create seven networks and compute the clustering coefficient, and the average shortest path length comparing them with those of the Erdős-Rényi model. Our analysis shows that all computed trajectory networks have the small world effect and the scale-free feature similar to the internet and biological networks. Finally, we discuss how these results could be interpreted in the light of the traffic application domain.
Simulated Annealing Algorithms for Optimal Packet Scheduling in Ad Hoc Networks

In this paper, we investigate simulated annealing algorithm (SA) capable of optimizing the scheduling tasks in ad hoc networks. They exploit the best features of the two well-known scheduling disciplines round robin (RR) and shortest distance (SD) based on local information at the nodes in the network. The effectiveness of the SA algorithms, where several cooling strategies are used, is evaluated by comparing system performance under different packet scheduling algorithms including RR and SD. Simulation results show that the proposed algorithms provide an optimized performance and minimize the average buffer size, therefore decreasing the power consumption of the system
ANALYSIS OF PHOTONIC BAND STRUCTURE IN ONE DIMENSIONAL PHOTONIC CRYSTAL USING PWE AND FDTD METHOD

A photonic crystal (PC) is said to be an artificially periodic layered structure that is known to possess photonic band gaps (PBGs) at certain frequency ranges. The photonic crystal consists of two alternating SNG materials, including that one has a negative permittivity (ENG) and the other has a negative permeability (MNG). In this paper we analyse band structure by computational method in single negative material by using plane wave expansion method and finite difference time domain method. Keywordsforbidden gap, PWE, FDTD, Eigen state, photonic band gap, single negative materials (SNGs).
Automatic selection of compiler options using genetic techniques for embedded software design

ROM size and CPU load are considered as critical resources for the software design process of the embedded software. Thus it is necessary to produce software that follows specific ROM and CPU load requirements. Compiler options play major role in the optimization of code size and CPU load of the software. Selection of the best compiler option-set that provides the required code size and CPU load is a challenging process due to the wide range of options provided by modern compilers. In this paper we are providing a new technique that enables the designers to select automatically the best compiler options set that matches their design requirements based on genetic techniques. We have also added a new genetics operator called pass-over operator to enhance the chromosomes selection for the next generation.
Conditions for Conformist Transmission C O N F O R M I S T T R a N S M I S S I O N Conditions for Conformist Transmission

Long before the origins of agriculture human ancestors had expanded across the globe into an immense variety of environments, from Australian deserts to Siberian tundra. Survival in these environments did not principally depend on genetic adaptations, but instead on evolved learning strategies that permitted the assembly of locally adaptive behavioral repertoires. To develop hypotheses about these learning strategies, we have modeled the evolution of learning strategies to assess what conditions and constraints favor which kinds of strategies. To build on prior work, we focus on clarifying how spatial variability, temporal variability, and the number of cultural traits influence the evolution of four types of strategies: (1) individual learning, (2) unbiased social learning, (3) payoff-biased social learning, and (4) conformist transmission. Using a combination of analytic and simulation methods, we show that spatial—but not temporal— variation strongly favors the emergence of conformist transmission. This effect intensifies when migration rates are relatively high and individual learning is costly. We also show that increasing the number of cultural traits above two favors the evolution of conformist transmission, which suggests that the assumption of only two traits in many models has been conservative. We close by discussing how (1) spatial variability represents only one way of introducing the low-level, non-adaptive phenotypic variation that so favors conformist transmission, the other obvious way being learning errors, and (2) our findings apply to the evolution of conformist transmission in social interactions. Throughout we emphasize how our models generate empirical predictions suitable for laboratory testing.
Simulation and Implementation Results of a 3-Phase 4-Wire Shunt Active Power Filter

This paper presents a laboratory prototype of a 3-phase 4-wire Shunt Active Power Filter used to compensate the source currents in an electrical system. The controller, based on the well known p-q theory was implemented on a DSP and determines the reference currents to be generated by a 2-level inverter. Some experimental results are presented and compared with computer simulations obtained with EMTDC/PSCAD V4.2. Other simulation results that demonstrate some of the improvements that will be made to the implemented prototype are also presented. HE widespread usage of non-linear loads like variable speed drives, switched power supplies, UPSs, rectifiers, and other industrial loads are known to deteriorate the power quality of the electrical systems. Problems such as harmonics, low power factor and imbalance can have dire consequences to the power distribution systems and the consumers (1). The utilization of switching semiconductors in equipments, which are responsible for introducing harmonic content on the electrical systems, can also be used to mitigate this and other power quality problems. In 1976 Gyugi and Strycula introduced the concept of Active Power Filters (2). This type of filters, contrarily to the passive ones, like LC filters or capacitor banks, has the capability to dynamically adapt to the conditions of the system in terms of harmonics and reactive power compensation, and additionally can also compensate imbalance. This paper deals with a laboratory prototype of a Shunt Active Filter for three-phase four-wire systems, which is capable of compensating current harmonics, reactive power and current imbalance. Some tests with the laboratory prototype are presented, and the acquired results are also compared with a simulation model developed with PSCAD. Improvements in the Shunt Active Filter are suggested and tested in the simulation model.
The impact of visitor segments on the perception of the quality of the product of accommodation establishments

The aim of the paper is to evaluate differences in the quality perception among particular segments of demand. Data for analysis were collected by means of the questionnaire survey among clients of accommodation establishments. The research in accommodation establishments succeeded to identify four factors of the perception of quality of services provided in those establishments, when only accommodation and catering services were taken into consideration. Age appeared to be an important criterion for the evaluation the of the quality of offered services as the differences in the quality perception among particular age groups were proved for three of four identified factors of the quality perception. The factors are as follows: “environment of the accommodation establishment”, “hygiene in accommodation establishment”, “service in the catering part of the establishment” and “quality of the meals”. The duration of stay as well as the gender of the respondents influences the quality of perception only in the perception of the “quality of meal” factor. Compared to the duration of stay, the repetition of the stay is a considerably important factor in causing the variability of the answers on the rate of the quality perception. The purpose of travel was also proven to be a criterion affecting the rate of the quality perception of the first three factors, whereas the impact of the criterion “client’s travel companionship” was proven in case of the first and third factor.
Assessment of child problem behaviors by multiple informants: a longitudinal study from preschool to school entry.

BACKGROUND Children's early problem behavior that manifests in multiple contexts is often more serious and stable. The concurrent and predictive validity of ratings of externalizing and internalizing by four informants was examined at preschool and early school age in an at-risk sample. METHODS Two hundred forty children were assessed by mothers and fathers (Child Behavior Checklist (CBCL)), and teachers and laboratory examiners (Teacher Report Form (TRF)) at ages 3 and 5 years. RESULTS All informants' ratings of externalizing converged on a common factor at ages 3 and 5 that showed strong stability over time (beta = .80). All informants' age 3 externalizing ratings significantly predicted the problem factor at age 5; mothers', fathers', and teachers' ratings were independently predictive. Ratings of internalizing (except by examiners at age 3) also converged at both ages; the problem factor showed medium stability (beta = .39) over time. Only fathers' ratings of age 3 internalizing predicted the age 5 problem factor. CONCLUSIONS Findings support the value of multi-informant assessment, uphold calls to include fathers in childhood research, and suggest that examiners provide valid, though non-unique assessment data. Examiner contributions may prove useful in many research contexts.
Cell-Free Replication of the Hepatitis C Virus Subgenomic Replicon

ABSTRACT The hepatitis C virus (HCV) contains a plus-strand RNA genome. The 5′ noncoding region (NCR) of the viral genome functions as an internal ribosome entry site, and its unique 3′ NCR is required for the assembly of the replication complex during initiation of HCV RNA replication. Lohmann et al. (V. Lohmann, F. Korner, J.-O. Koch, U. Herian, L. Theilman, and R. Batenschlager, Science 285:110-113, 1999) developed a subgenomic HCV replicon system, which represents an important tool in studying HCV replication in cultured cells. In this study, we describe a cell-free replication system that utilizes cytoplasmic lysates prepared from Huh-7 cells harboring the HCV subgenomic replicons. These lysates, which contain ribonucleoprotein complexes associated with cellular membranes, were capable of incorporating [α32P]CTP into newly synthesized RNA from subgenomic replicons in vitro. Replicative forms (RFs) and replicative intermediates (RIs) were synthesized from the endogenous HCV RNA templates. Consistent with previous observations, RFs were found to be resistant to RNase A digestion, whereas RIs were sensitive to RNase treatment. The radiolabeled HCV RF-RI complexes contained both minus and plus strands and were specific to the lysates derived from replicon-expressing cells. The availability of a cell-free replication system offers opportunities to probe the mechanism(s) of HCV replication. It also provides a novel assay for potential therapeutic agents.
The Current Implementation Status of Manufacturing Control Systems for a Key Manufacturing Industry

Manufacturing, production and service industries within Libya have struggled with many problems during the past two decades due to many difficulties. These problems have created a negative impact on the productivity and utilization of many industries around the country. This paper studies the implementation levels of the manufacturing control systems known as Manufacturing Resource Planning (MRPII) being adapted within some Libyan industries. A survey methodology has been applied for this research, based on the survey analysis, the results pointed out that the system within these industries has a modest strategy towards most of the areas that are considered as being very crucial in implementing these systems successfully. The findings also show a variation within these implementation levels with a respect to the key-elements that related to MRPII, giving the highest levels in the emphasise on financial data accuracy. The paper has also identified limitations within the investigated manufacturing and managerial areas and has pointed to where senior managers should take immediate actions in order to achieve effective implementation of MRPII within their business area. Keywords—Control, Industry, Manufacturing, Survey, System
Classification of Opinions with Non-affective Adverbs and Adjectives

We propose domain-independent language patterns that purposefully omit the aective words for the classification of opinions. The information extracted with those patterns is then used to analyze opinions expressed in the texts. Empirical evidence shows that opinions can be discovered without the use of aective words. We ran experiments on four sets of reviews of consumer goods: books, DVD, electronics, kitchen, and house ware. Our results support the practical use of our approach and its competitiveness in comparison with other data-driven methods. This method can also be applied to analyze texts which do not explicitly disclose aects such as medical and legal documents.
Nanoribbons from Conjugated Macromolecules on Amorphous Substrates Observed by SFM and TEM

Micrometre long nanoribbons have been grown from solutions of functionalized poly( para-phenyleneethynylene)s (PPE)s on noncrystalline insulating substrates including glass and carbon coated copper grids. Tapping mode scanning force microscopy (SFM) and transmission electron microscopy (TEM) revealed that these nanostructures possess a molecular cross section with a typical thickness of 2-3 molecular layers and a width which reflects the distribution of macromolecular lengths. The ribbons are therefore quite similar to the ones found on the crystalline mica substrate except that they are not oriented within the surface plane. This indicates that the growth of these architectures from solution is mainly governed by intermolecular interactions between the π-conjugated macromolecules. The possibility to self-assemble these nanoribbons also on amorphous silica opens a prospect for their application as molecular nanowires bridging gold nanoelectrodes grown on oxidized silicon wafers.
Memory Access Characterization of OpenMP Workloads on a Multi-core NUMA Machine

Nowadays, on hierarchical shared memory multiprocessors with Non-Uniform Memory Access (NUMA), the number of cores accessing memory banks is considerably high. Such accesses produce more stress on the memory banks, generating load-balancing issues, memory contention and remote accesses. In this context, it is important to have a good understanding of memory access patterns and what are the inuences of data placement on such patterns. In this document, we have investigated memory accesses behavior of microbenchmarks and benchmarks over a ccNUMA platform with multi-core processors. Additionally, we have evaluated a set of memory policies that were used to place data among the machine memory banks. Our results have shown that an appropriate selection of data placement, considering the memory accesses, can generated great improvement gains.
Insomnia among Patients with Chronic Heart Failure

Objective: Although death anxiety has been well studied in the past, there seems to be a lack of understanding its importance in both simple and complex anxiety. The emotional complexity and persistence of trauma reactions in veterans may be better understood in the context of intrapsychic sources and related anxieties of those traumas, including fear of death. The purpose of this study was to address the significance of death anxiety in understanding and treating PTSD. The primary hypotheses of this study focused on the relationship of death anxiety with PTSD, depression, and anxiety. Methods: This study utilized a correlational design, and examined the relationship between traumatic stress and death anxiety and some of the sequelae (depression and anxiety) to determine whether there are relationships of any significance. Numerous measures were utilized including: Death Anxiety Scale, PTSD Checklist for Military, Depression Anxiety Stress Scales, and demographics questions. Results: This study surveyed both veterans and active duty military using snowball sampling as well as recruitment through college support groups and Veterans of Foreign Wars. Participants for this study consisted of 42 individuals serving in four military branches. Of the outcomes expected, two showed non-significant positive relationships while the third indicated a statistically significant positive relationship to death anxiety. Conclusions: The relationship found between death anxiety and PTSD agreed with past theories that suggested death anxiety is the root of all anxiety and that past experiences with death can lead to the development of PTSD.