Miracle-dz/UniFAR-FTUs huggingface dataset:
Mouse Zac1, a Transcriptional Coactivator and Repressor for Nuclear Receptors

ABSTRACT Transcriptional activation by nuclear hormone receptors is mediated by the 160-kDa family of nuclear receptor coactivators. These coactivators associate with DNA-bound nuclear receptors and transmit activating signals to the transcription machinery through two activation domains. In screening for mammalian proteins that bind the C-terminal activation domain of the nuclear receptor coactivator GRIP1, we identified a new variant of mouse Zac1 which we call mZac1b. Zac1 was previously discovered as a putative transcriptional activator involved in regulation of apoptosis and the cell cycle. In yeast two-hybrid assays and in vitro, mZac1b bound to GRIP1, to CREB-binding protein (CBP) and p300 (which are coactivators for nuclear receptors and other transcriptional activators), and to nuclear receptors themselves in a hormone-independent manner. In transient-transfection assays mZac1b exhibited a transcriptional activation activity when fused with the Gal4 DNA binding domain, and it enhanced transcriptional activation by the Gal4 DNA binding domain fused to GRIP1 or CBP fragments. More importantly, mZac1b was a powerful coactivator for the hormone-dependent activity of nuclear receptors, including androgen, estrogen, glucocorticoid, and thyroid hormone receptors. However, with some reporter genes and in some cell lines mZac1b acted as a repressor rather than a coactivator of nuclear receptor activity. Thus, mZac1b can interact with nuclear receptors and their coactivators and play both positive and negative roles in regulating nuclear receptor function.
Dental caries risk indicators among Australian Aboriginal young adults.

OBJECTIVES To determine dental caries risk indicators among a birth cohort of Australian Aboriginal young adults (n=442). METHODS Data were from the Aboriginal Birth Cohort study, a prospective longitudinal investigation of Aboriginal individuals born 1987-1990 at an Australian regional hospital. Models representing demographic, socioeconomic, behavioural, dental service utilization and clinical oral health variables were tested using multivariate regression. RESULTS The percent DT>0 was 72.9 (95% CI 68.7-77.1), mean DT was 4.19 (95% CI 3.8-4.6), percent DMFT>0 was 77.4 (95% CI 73.5-81.3) and mean DMFT was 4.84 (95% CI 4.4-5.3). After controlling for other covariates, risk indicators for percent DT>0 included soft drink consumption every day or a few times a week (PR 1.25, 95% CI 1.08-1.45), not consuming milk every day or a few times a week (PR 1.16, 95% CI 1.04-1.30) and sweet consumption every day or a few times a week (PR 1.18, 95% CI 1.04-1.33). Risk indicators for mean DT included sweet consumption every day or a few times a week (B=1.14, 95% CI 0.27-2.02), nonownership of a toothbrush (B=0.91, 95% CI 0.10-1.87) and presence of plaque (B=2.46, 95% CI 0.96-3.96). Those with 4 + occupants in their house the previous night had 1.2 times the prevalence of having DMFT>0 than their counterparts with less household occupants (95% CI 1.01-1.49). Percent DMFT>0 was also associated with consumption of soft drink every day or a few times a week (PR 1.18, 95% CI 1.04-1.34) and consumption of sweets every day or a few times a week (PR 1.23, 95% CI 1.10-1.37). Mean DMFT was higher among those who consumed sweets every day or a few times a week (B = 0.13, 95% CI 0.05-0.22) and who had dental anxiety (B=0.10, 95% CI 0.01-0.19). CONCLUSIONS In an Australian Aboriginal young adult cohort, risk indicators for dental caries included social determinants such as household size, dietary behaviours such as regular consumption of soft drink and sweets, dental behaviour such as nonownership of a toothbrush and dental anxiety.
Creative Activity Technology , School of 1-1-2007 Performance Analysis of Security Aspects in UML Models

The focus of the paper is on the analysis of performance effects of different security solutions modeled as aspects in UML. Aspect oriented modeling (AOM) allows software designers to isolate and separately address solutions for crosscutting concerns, which are defined as distinct UML aspect models, then are composed with the primary UML model of the system under development. For performance analysis we use techniques developed previously in the PUMA project, which take as input UML models annotated with the standard UML Profile for Schedulability, Performance and Time (SPT), and transform them first into Core Scenario Model (CSM) and then into different performance models. The contribution of this paper is in performing the composition of the aspects with the primary model at the CSM level. The input is represented by the primary model and a number of aspect models in UML+SPT, which are processed as follows: a) converted separately to CSM; b) composed into a single CSM model; c) transformed into a Layered Queueing Networks (LQN) model and d) analyzed. The proposed approach is illustrated with a case study based on two standards, TPC-W and SSL.
A Case Study Examining Change in Teacher Beliefs Through Collaborative Action Research

The main goal of this study was to explore the role of collaborative action research in eliciting change in teacher beliefs. The beliefs were those of five chemistry teachers in implementing a new teaching approach, geared to enhancing students' scientific and technological literacy (STL). The teacher beliefs were analysed based on Ajzen's Theory of Planned Behaviour (2005) by looking at the teacher's (a) attitude towards implementing STL modules, (b) perceived subjective norms, and (c) behavioural control regarding the new teaching approach. After an introductory year, when teachers familiarised themselves with the new approach, a collaborative action research project was initiated in the second year of the study, helping teachers to minimise or overcome initially perceived constraints when implementing STL modules in their classroom. The processes of teacher change and the course of the project were investigated by teacher interviews, teacher informal commentaries, and meeting records. The formation of positive beliefs towards a STL approach increased continuously, although its extent and character varied depending on the teacher. The close cooperation, in the format of collaborative action research and especially through teacher group reflections and perceived collegial support, did support teacher professional development including change in their beliefs towards the new teaching approach. Additionally, positive feedback gained from other teachers through running a two-day in-service course in year three helped to strengthen all five teachers' existing beliefs towards the new approach. The current research demonstrated that perceived constraints, where identified, can be meaningfully addressed by teachers, through undertaking collaborative action research.
DIAGNOSIS AND IMPLICATIONS OF MYCOBACTERIUM BOVIS INFECTION IN BANDED MONGOOSES (MUNGOS MUNGO) IN THE KRUGER NATIONAL PARK, SOUTH AFRICA

Abstract Bovine tuberculosis (bTB) was first diagnosed in the Kruger National Park (KNP) in 1990. Research has since focused on the maintenance host, the African buffalo (Syncerus caffer) and clinically affected lion (Panthera leo). However, little is known about the role of small predators in tuberculosis epidemiology. During 2011–12, we screened banded mongooses (Mungos mungo) in the bTB high-prevalence zone of the KNP for Mycobacterium tuberculosis complex members. Fecal swabs, tracheal swabs, and tracheal lavages of 76 banded mongooses caught in cage traps within a 2-km radius of Skukuza Rest Camp were submitted for Mycobacterium culture, isolation, and species identification. Lesions and lymph node samples collected from 12 animals at postmortem examination were submitted for culture and histopathology. In lung and lymph nodes of two banded mongooses, well demarcated, irregularly margined, gray-yellow nodules of up to 5 mm diameter were identified with either central necrosis or calcification, characterized on histopathology as caseating necrosis with epithelioid macrophages or necrogranuloma with calcified centre. No acid fast bacteria were identified with Ziehl–Neelsen stain. We isolated Mycobacterium bovis from lung, lymph node, and liver samples, as well as from tracheal lavages and tracheal swab from the same two banded mongooses. Blood samples were positive by ElephantTB STAT-PAK® Assay for 12 and Enferplex™ TB Assay for five animals. Only the two banded mongooses positive on pathology and M. bovis culture were positive on both serologic assays. We provide evidence of bTB infection in banded mongooses in the KNP, demonstrate their ability to shed M. bovis, and propose a possible antemortem diagnostic algorithm. Our findings open the discussion around possible sources of infection and their significance at the human/wildlife interface in and around Skukuza.
Computer-based Assessment of Problem Solving Competence

Abstract Problem solving competence cannot be described as a unidimensional construct. At least analytical and dynamic aspects of problem solving competence have to be distinguished if all aspects of problem solving are to be covered. Analytical problem solving abilities are needed to structure, represent and integrate information. Dynamic problem solving includes aspects of self-regulated learning as well as the ability to adapt the problem solving process to a changing environment by continuously processing feedback information. The assessment of dynamic aspects of problem solving competence requires dynamic test environments. As a German national option of PISA 2000, the analytical and dynamic problem solving competencies of 15-year-old students were tested using paper-and-pencil tests as well as computer-based assessments. Results show that analytical aspects of problem solving competence are strongly correlated with reasoning, while dynamic problem solving reflects a specific dimension of self-regulated exploration and control that can be identified across computer-simulated domains.
Bricolages et Métissages. La construction d'une méthode multi-référentielle dans une recherche sur l'espace thérapeutique en thérapie interculturelle

Nous discutons les defis de la construction d’une methodologie interdisciplinaire en psychologie interculturelle a partir d’une recherche sur une psychotherapie transculturelle dans un dispositif groupal. La recherche analyse les interactions dans un dispositif specialise pour les therapies transculturelles en France. L’objectif de la recherche est de mieux comprendre comment therapeutes et patients co-construisent un dialogue sur la culture, les appartenances et les representations culturelles, et comment ils arrivent a faire une mediation entre differents univers culturels. Au niveau methodologique, nous avons croise des elements venant de l’anthropologie interpretative, de l’ethnopsychanalyse et de la theorie ancree. La demarche interdisciplinaire demande necessairement une part de creativite qui favorise la decouverte et la nuance dans la description des processus d’echange interculturel.
Nebulized but not parenteral IFN-gamma decreases IgE production and normalizes airways function in a murine model of allergen sensitization.

An animal model of local allergen (airways) sensitization was employed to study the effects of rIFN-gamma administered by ultrasonic nebulization through the airways on IgE production and airways responsiveness. BALB/c mice exposed to aerosolized OVA daily for 10 days developed a predominant anti-OVA IgE response, immediate cutaneous reactivity to OVA, and increased airways responsiveness (AR). Mice were treated with rIFN-gamma, either systemically or locally via the airways, following different protocols; i.p. rIFN-gamma failed to modulate the course of OVA sensitization, although total IgE levels in the serum were decreased by 50%. Anti-OVA IgE levels remained elevated, immediate skin test responses to OVA persisted, and AR was increased. However, local treatment of the airways with nebulized rIFN-gamma caused a 66% decrease in serum anti-OVA IgE and a twofold rise in IgG2a levels. Cutaneous reactivity to OVA was reduced and AR was also normalized after nebulized rIFN-gamma. In contrast to the i.p. route, treatment with nebulized rIFN-gamma resulted in a reduction in the in vitro IgE production by lymphocytes in response to OVA and IL-4. The timing of treatment with nebulized rIFN-gamma was important in determining the immunomodulatory response. Treatment after day 7 of OVA exposure failed to modulate sensitization. Treatment regimens with nebulized rIFN-gamma that began before day 7 of OVA exposure were able to decrease anti-OVA IgE. Only treatment regimens that included 3 days of nebulized IFN-gamma before OVA sensitization caused a decrease in cutaneous reactivity and normalization of AR. The data demonstrate that both the route and timing of rIFN-gamma administration are critical factors in the immunomodulation of the immediate allergic response to allergen sensitization via the airways.
Impact of PEVs on automatic generation control using TLBO algorithm

In this paper Proportional-Integral-Derivative (PID) gains are evaluated by Teaching Learning Based Optimization (TLBO) algorithm. Three area thermal system of Automatic Generation Control (AGC) are considered with PlugIn Electric Vehicles (PEVs) to solve load frequency control problem. Incorporation of PEVs the frequency and tie-line power response are improved in terms of undershoot (US), overshoot (OS), settling time (ST) and damping ratio (df/dt). These PID gain values are found analytically by using the MATLAB SOFTWARE based program. The frequency and tie-line power transient responses are verified by using MATLAB SIMULINK SOFTWARE. The comparison of frequency and tie-line power response characteristics with and without PEVs are also mentioned in this paper.
Self-determination and bilingualism

This article focuses on additive bilingualism for minority group children, more specifically the development of strong literacy skills in English and in the children's language. The personal autonomization language learning (PALL) model is presented. It specifies eight testable hypotheses. Self-determination theory (SDT) is central in the PALL model. It is argued that autonomy support in both languages is related to basic needs satisfaction (autonomy, competence, relatedness: ACR) which is in turn related to internally regulated motivation for learning the language. Owing to the strong social attraction of English, the model proposes that favouring the learning of the minority language and basic needs satisfaction in that language foster additive bilingualism because of the strong interlinguistic transfer of minority language competencies and of an internal motivational orientation. Self-determination to learn the minority language and minority literacy skills seems to transfer across languages. Research results support the model; these are summarized and educational implications are discussed.
Differing Mechanisms of Death Induction by Fluorinated Taxane SB-T-12854 in Breast Cancer Cells.

BACKGROUND/AIM Classical taxanes are routinely used in cancer therapy. In this study, mechanisms involved in death induction by the novel fluorine-containing taxane SB-T-12854 were investigated. MATERIALS AND METHODS We employed breast cancer SK-BR-3, MCF-7 and T47D cell lines to assess activation of individual caspases, changes in the expression of proteins of the Bcl-2 family, and the release of pro-apoptotic factors from mitochondria into the cytosol after SB-T-12854 treatment. RESULTS Caspase-2, -8, and -9 were activated in SK-BR-3 and MCF-7 cells. Only caspase-8 was activated in T47D cells. Caspase-7 and -6 were activated in all tested cells while caspase-3 was activated only in SK-BR-3 cells. Pro-apoptotic Bad protein seems to be important for cell death induction in all tested cells. Anti-apoptotic Bcl-2 and pro-apoptotic Bim, Bok, Bid and Bik seem to be also associated with cell death induction in some of the tested cells. The mitochondrial apoptotic pathway was significantly activated in association with the release of cytochrome c and Smac from mitochondria, but only in SK-BR-3 cells, not in MCF-7 and T47D cells. CONCLUSION Cell death induced by SB-T-12854, in the tested breast cancer cells, differs regarding activation of caspases, changes in levels of pro-apoptotic and anti-apoptotic proteins of the Bcl-2 family and activation of the mitochondrial apoptotic pathway.
Conjugal Power Relations and Couples’ Participation in Reproductive Health Decision-Making: Exploring the Links in Nepal

Abstract This article appraises conjugal power relations and explores the nexus between such relations and couples’ participation in reproductive health (RH) decision-making based on a field survey carried out in two population clusters in eastern Nepal. The findings show that husbands’ domination is ubiquitous with respect to all forms of power and gender privileges, which accords with a dominant-submissive model of conjugal relationship. However, decision-making in RH shows a more complex interaction between different sets of factors of power. Generally, family planning is perceived as a couple’s private issue whereas ante-natal care (ANC) is considered to be exclusively a woman’s health issue. High rates of women’s participation in joint contraceptive decision-making may be taken as encouraging. Yet, husbands’ domination is evident in directing wives to use contraceptives, choose their types and to terminate their application and in making decisions about seeking ANC service. Gender difference in RH decision-making is strongly attributable to unequal gender power relations, traditional gender roles and the financial cost associated with such a service. Since women’s low status and the existing hierarchical system are among the major factors that maintain unequal conjugal relations, integration of women’s empowerment and transformation of gender ideology into policies and programs would be one of the vital entry points for achieving an equitable and respectful conjugal partnership to enable women to claim their sexual and reproductive rights and ultimately to make their own informed decisions.
Gabapentin-associated hepatotoxicity

we can attempt an ERCP without radiopaque media, using the technique of air contrastography. The essential phase is the deep cannulation of the bile duct, and it is very important that the endoscopist has a high success rate in the cannulation of the selected duct. The sphincterotomy can be performed with the usual technique. Subsequent contrastography can be obtained with the aid of a Fogarty balloon. The quantity of air to insufflate must be high (100–150 cc) for a successful air contrastography. If the treatment of lithiasis is not difficult, and thus not requiring repeated air contrastography, the ERCP procedure can be performed in the same time as the standard procedure that uses contrastography with radiopaque media for the same type of bile duct lithiasis. In the case of patients with acute disease and severe previous reaction to contrast media or where the patient with an allergic diathesis refuses to give informed consent to the use of iodinate contrast, any biliopancreatic endoscopist should be aware of the possibility of performing urgent ERCP with the safe and successful air contrastography, without the need to await adequate pharmacological preparation.
Identification of the difference in the pathogenesis in heart failure arising from different etiologies using a microarray dataset

OBJECTIVES: Clinically, patients with chronic heart failure arising from different etiologies receive the same treatment. However, the prognoses of these patients differ. The purpose of this study was to elucidate whether the pathogenesis of heart failure arising from different etiologies differs. METHODS: Heart failure-related dataset GSE1145 was obtained from the Gene Expression Omnibus database. Differentially expressed genes were identified using R. A protein-protein interaction network of the differentially expressed genes was constructed using Search Tool for the Retrieval of Interacting Genes. The modules in each network were analyzed by Molecular Complex Detection of Cytoscape. The Database for Annotation, Visualization and Integrated Discovery was used to obtain the functions of the modules. RESULTS: Samples contained in GSE1145 were myocardial tissues from patients with dilated cardiomyopathy, familial cardiomyopathy, hypertrophic cardiomyopathy, ischemic cardiomyopathy, and post-partum cardiomyopathy. The differentially expressed genes, modules, and functions of the modules associated with different etiologies varied. Abnormal formation of extracellular matrix was overlapping among five etiologies. The change in cytoskeleton organization was specifically detected in dilated cardiomyopathy. The activation of the Wnt receptor signaling pathway was limited to hypertrophic cardiomyopathy. The change in nucleosome and chromatin assembly was associated with only familial cardiomyopathy. Germ cell migration and disrupted cellular calcium ion homeostasis were solely detected in ischemic cardiomyopathy. The change in the metabolic process of glucose and triglyceride was detected in only post-partum cardiomyopathy. CONCLUSION: These results indicate that the pathogenesis of heart failure arising from different etiologies varies, which may provide molecular evidence supporting etiology-based treatment for heart failure patients.
The Detection, Surveillance and Treatment of Premalignant Gastric Lesions Related to Helicobacter pylori Infection

Gastric cancer is an important worldwide health problem and causes considerable morbidity and mortality. It represents the second leading cause of cancer‐related death worldwide. A cascade of recognizable precursor lesions precedes most distal gastric carcinomas. In this multistep model of gastric carcinogenesis, Helicobacter pylori causes chronic active inflammation of the gastric mucosa, which slowly progresses through the premalignant stages of atrophic gastritis, intestinal metaplasia and dysplasia to gastric carcinoma. Detection and treatment of premalignant lesions may thus provide a basis for gastric cancer prevention. However, at present, premalignant changes of the gastric mucosa are frequently disregarded in clinical practice or result in widely varying follow‐up frequency or treatment. This review provides an overview of current knowledge on detection, surveillance and treatment of patients with premalignant gastric lesions, and identifies the uncertainties that require further research.