Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Apple Juice Enrichment with Selected Plant Materials: Focus on Bioactive Compounds and Antioxidant Activity

Using a multi-analytical approach, this paper aimed to investigate the effect of apple juice enrichment with Arbutus unedo and Diospyros kaki fruits, Myrtus communis berry extract, Acca sellowiana, or Crocus sativus flower by-products on both bioactive compounds content and antioxidant activity. Physico-chemical parameters, vitamin C, sugars, organic acids, total polyphenol content, antioxidant activity, and sensory attributes were evaluated. An LC-PDA/MS QTof analysis allowed for the identification of 80 different phenolic compounds. The highest polyphenol content (179.84 and 194.06 mg of GAE/100 g fw) and antioxidant activity (CUPRAC, 6.01 and 7.04 mmol of Fe2+/100 g fw) were observed in products with added A. sellowiana and D. kaki, respectively. Furthermore, the study showed a positive correlation between polymeric procyanidins and antioxidant activity (0.7646–0.8539). The addition of A. unedo fruits had a positively significant influence on the increment of vitamin C (23.68 ± 0.23 mg/100 g fw). The obtained products were attractive to consumers, especially those with 0.1% C. sativus flower juice, M. communis berry extract, and persimmon D. kaki fruits. The synergy among the different analytical techniques allowed us to obtain a complete set of information, demonstrating that the new apple smoothies were enriched in both different beneficial molecules for human health and in antioxidant activity.
How hospital top managers reason about the central leadership task of balancing quality of patient care, economy and professionals’ engagement: an interview study

Purpose This study aims to deepen the understanding of how top managers reason about handling the relationships between quality of patient care, economy and professionals’ engagement. Design/methodology/approach Qualitative design. Individual in-depth interviews with all members of the executive management team at an emergency hospital in Norway were analysed using reflexive thematic method. Findings The top managers had the intention to balance between quality of patient care, economy and professionals’ engagement. This became increasingly difficult in times of high internal or external pressures. Then top management acted as if economy was the most important focus. Practical implications For health-care top managers to lead the pursuit towards increased sustainability in health care, there is a need to balance between quality of patient care, economy and professionals’ engagement. This study shows that this balancing act is not an anomaly top-managers can eradicate. Instead, they need to recognize, accept and deliberately act with that in mind, which can create virtuous development spirals where managers and health-professional communicate and collaborate, benefitting quality of patient care, economy and professionals’ engagement. However, this study builds on a limited number of participants. More research is needed. Originality/value Sustainable health care needs to balance quality of patient care and economy while at the same time ensure professionals’ engagement. Even though this is a central leadership task for managers at all levels, there is limited knowledge about how top managers reason about this.
ACADEMIC STRESS AND GRATITUDE TO PROMOTE STUDENT WELL-BEING

Research in education is developing very rapidly and widely, not only examining the cognitive domain but also starting to develop into the affective domain, such as research on student well-being. This study aimed to examine the correlation between academic stress and gratitude with student well-being. The approach used in this research is correlational quantitative. The research scale is given offline and online using a google form. Respondents in this study were 602 students of Madrasah Aliyah Pekanbaru, Riau, Indonesia. The instruments used are the academic stress, gratitude and student well-being scale. The results of data analysis with multiple regression analysis showed that there was a significant relationship between academic stress and gratitude with student well-being (p-value < 0.001; r 0.655), meaning that the hypothesis is accepted, with the effective contribution of academic stress and gratitude simultaneously on student well-being by 42.9%. Academic stress were negatively and significantly associated with student well-being when the gratitude variable was controlled (p < 0.001; Beta -0.521), and gratitude were positively and significantly associated with student well-being when the academic stress variable was controlled (p < 0.001; Beta 0.302). Furthermore, in qualitative data, the researcher found that some students felt stressed with the workload from school because they did not understand the subject matter, communication with teachers was not fluent, anxious and sad, afraid to disappoint parents, and dizzy and frustrated in thinking about the subject matter. The researcher recommends that schools organize training that can reduce academic stress and increase student gratitude, so that student well-being can increase.
A Lab-Made E-Nose-MOS Device for Assessing the Bacterial Growth in a Solid Culture Medium

The detection and level assessment of microorganisms is a practical quality/contamination indicator of food and water samples. Conventional analytical procedures (e.g., culture methods, immunological techniques, and polymerase chain reactions), while accurate and widely used, are time-consuming, costly, and generate a large amount of waste. Electronic noses (E-noses), combined with chemometrics, provide a direct, green, and non-invasive assessment of the volatile fraction without the need for sample pre-treatments. The unique olfactory fingerprint generated during each microorganism’s growth can be a vehicle for its detection using gas sensors. A lab-made E-nose, comprising metal oxide semiconductor sensors was applied, to analyze solid medium containing Gram-positive (Enterococcus faecalis and Staphylococcus aureus) or Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The electrical-resistance signals generated by the E-nose coupled with linear discriminant analysis allowed the discrimination of the four bacteria (90% of correct classifications for leave-one-out cross-validation). Furthermore, multiple linear regression models were also established allowing quantifying the number of colony-forming units (CFU) (0.9428 ≤ R2 ≤ 0.9946), with maximum root mean square errors lower than 4 CFU. Overall, the E-nose showed to be a powerful qualitative–quantitative device for bacteria preliminary analysis, being envisaged its possible application in solid food matrices.
In Vivo Safety and Efficacy of Chalcone-Loaded Microparticles with Modified Polymeric Matrix against Cutaneous Leishmaniasis

Current chemotherapy of cutaneous leishmaniasis (CL) is based on repeated systemic or intralesional administration of drugs that often cause severe toxicity. Previously, we demonstrated the therapeutic potential of biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs) loaded with 8% of the nitrochalcone CH8 (CH8/PLGA) prepared by a conventional bench method. Aiming at an industrially scalable process and increased drug loading, new MPs were prepared by spray drying: CH8/PDE with PLGA matrix and CH8/PVDE with PLGA + polyvinylpyrrolidone (PVP) matrix, both with narrower size distribution and higher drug loading (18%) than CH8/PLGA. Animal studies were conducted to evaluate their clinical feasibility. Both MP types induced transient local swelling and inflammation, peaking at 1–2 days, following a single intralesional injection. Different from CH8/PDE that released 90% of the drug in the ear tissue in 60 days, CH8/PVDE achieved that in 30 days. The therapeutic efficacy of a single intralesional injection was evaluated in BALB/c mice infected with Leishmania (Leishmania) amazonensis and golden hamsters infected with L. (Viannia) braziliensis. CH8/PVDE promoted greater reduction in parasite burden than CH8/PDE or CH8/PLGA, measured at one month and two months after the treatment. Thus, addition of PVP to PLGA MP matrix accelerates drug release in vivo and increases its therapeutic effect against CL.
Characterization of Diabetic Retinopathy in Two Mouse Models and Response to a Single Injection of Anti-Vascular Endothelial Growth Factor

In this study, we characterized diabetic retinopathy in two mouse models and the response to anti-vascular endothelial growth factor (VEGF) injection. The study was conducted in 58 transgenic, non-obese diabetic (NOD) mice with spontaneous type 1 diabetes (n = 30, DMT1-NOD) or chemically induced (n = 28, streptozotocin, STZ-NOD) type 1 diabetes and 20 transgenic db/db mice with type 2 diabetes (DMT2-db/db); 30 NOD and 8 wild-type mice served as controls. Mice were examined at 21 days for vasculopathy, retinal thickness, and expression of genes involved in oxidative stress, angiogenesis, gliosis, and diabetes. The right eye was histologically examined one week after injection of bevacizumab, ranibizumab, saline, or no treatment. Flat mounts revealed microaneurysms and one apparent area of tufts of neovascularization in the diabetic retina. Immunostaining revealed activation of Müller glia and prominent Müller cells. Mean retinal thickness was greater in diabetic mice. RAGE increased and GFAP decreased in DMT1-NOD mice; GFAP and SOX-9 mildly increased in db/db mice. Anti-VEGF treatment led to reduced retinal thickness. Retinas showed vasculopathy and edema in DMT1-NOD and DMT2-db/db mice and activation of Müller glia in DMT1-NOD mice, with some response to anti-VEGF treatment. Given the similarity of diabetic retinopathy in mice and humans, comparisons of type 1 and type 2 diabetic mouse models may assist in the development of new treatment modalities.
A class of interacting marked point processes: rate of convergence to equilibrium

In this paper we obtain the rate of convergence to equilibrium of a class of interacting marked point processes, introduced by Kerstan, in two different situations. Indeed, we prove the exponential and subexponential ergodicity of such a class of stochastic processes. Our results are an extension of the corresponding results of Brémaud, Nappo and Torrisi. The generality of the dynamics which we take into account allows the application to the so-called loss networks, and multivariate birth and death processes.
A Validation of a Satellite Cloud Retrieval during ASTEX

Abstract An algorithm using NOAA-12 AVHRR (Advanced Very High Resolution Radiometer) solar reflectance measurements for retrieving cloud droplet size and optical thickness has been applied to a boundary layer stratocumulus cloud in the vicinity of the Azores on 12 June 1992 during the Atlantic Stratocumulus Transition Experiment (ASTEX). This day was particularly advantageous for validations because of the absence of cirrus or other higher-level clouds during the satellite overpass and the existence of a large relatively uniform stratus cloud dock. Uncertainty estimates for the retrievals are presented along with a discussion of the algorithm. An in-flight absolute calibration of AVHRR channel 1, necessary for accurate optical thickness retrievals, was done concurrently with the retrievals through comparison with a visible radiometer flown on the National Aeronautics and Space Administration's ER-2 and using the stratus cloud as the common reflectance target. Results are compared with in situ microphysica.
Active Actions in the Extraction of Urban Objects for Information Quality and Knowledge Recommendation with Machine Learning

Due to the increasing urban development, it has become important for municipalities to permanently understand land use and ecological processes, and make cities smart and sustainable by implementing technological tools for land monitoring. An important problem is the absence of technologies that certify the quality of information for the creation of strategies. In this context, expressive volumes of data are used, requiring great effort to understand their structures, and then access information with the desired quality. This study are designed to provide an initial response to the need for mapping zones in the city of Itajaí (SC), Brazil. The solution proposes to aid object recognition employing object-based classifiers OneR, NaiveBayes, J48, IBk, and Hoeffding Tree algorithms used together with GeoDMA, and a first approach in the use of Region-based Convolutional Neural Network (R-CNN) and the YOLO algorithm. All this is to characterize vegetation zones, exposed soil zones, asphalt, and buildings within an urban and rural area. Through the implemented model for active identification of geospatial objects with similarity levels, it was possible to apply the data crossover after detecting the best classifier with accuracy (85%) and the kappa agreement coefficient (76%). The case study presents the dynamics of urban and rural expansion, where expressive volumes of data are obtained and submitted to different methods of cataloging and preparation to subsidize rapid control actions. Finally, the research describes a practical and systematic approach, evaluating the extraction of information to the recommendation of knowledge with greater scientific relevance. Allowing the methods presented to apply the calibration of values for each object, to achieve results with greater accuracy, which is proposed to help improve conservation and management decisions related to the zones within the city, leaving as a legacy the construction of a minimum technological infrastructure to support the decision.
Taylor vortices between eccentric cylinders

The critical Taylor number and the critical wavelength of Taylor vortices has been measured as a function of the eccentricity. It has been found that in this apparatus the critical Taylor number increases steadily as a function of eccentricity. The critical wavelength was found to remain nearly constant for small e and to decrease with larger e. Separated flow, as observed from above, was found to be in qualitative agreement with the prediction of Kamal.
Beneficial Effect of ACI-24 Vaccination on Aβ Plaque Pathology and Microglial Phenotypes in an Amyloidosis Mouse Model

Amyloid-β (Aβ) deposition is an initiating factor in Alzheimer’s disease (AD). Microglia are the brain immune cells that surround and phagocytose Aβ plaques, but their phagocytic capacity declines in AD. This is in agreement with studies that associate AD risk loci with genes regulating the phagocytic function of immune cells. Immunotherapies are currently pursued as strategies against AD and there are increased efforts to understand the role of the immune system in ameliorating AD pathology. Here, we evaluated the effect of the Aβ targeting ACI-24 vaccine in reducing AD pathology in an amyloidosis mouse model. ACI-24 vaccination elicited a robust and sustained antibody response in APPPS1 mice with an accompanying reduction of Aβ plaque load, Aβ plaque-associated ApoE and dystrophic neurites as compared to non-vaccinated controls. Furthermore, an increased number of NLRP3-positive plaque-associated microglia was observed following ACI-24 vaccination. In contrast to this local microglial activation at Aβ plaques, we observed a more ramified morphology of Aβ plaque-distant microglia compared to non-vaccinated controls. Accordingly, bulk transcriptomic analysis revealed a trend towards the reduced expression of several disease-associated microglia (DAM) signatures that is in line with the reduced Aβ plaque load triggered by ACI-24 vaccination. Our study demonstrates that administration of the Aβ targeting vaccine ACI-24 reduces AD pathology, suggesting its use as a safe and cost-effective AD therapeutic intervention.
Subpicosecond switching with symmetric Mach-Zehnder all-optical switch

Summary form only given. Subpicosecond switching with the symmetric Mach-Zehnder (SMZ) all-optical switch has been demonstrated. The minimum full-width at half-maximum of the cross-correlation trace is less than 1 ps. To our best knowledge, this is the fastest switching experiment with incoherent and thus realistic, efficient nonlinearities.
Epimeric Mixture Analysis and Absolute Configuration Determination Using an Integrated Spectroscopic and Computational Approach—A Case Study of Two Epimers of 6-Hydroxyhippeastidine

Structural elucidation has always been challenging, and misassignment remains a stringent issue in the field of natural products. The growing interest in discovering unknown, complex natural structures accompanies the increasing awareness concerning misassignments in the community. The combination of various spectroscopic methods with molecular modeling has gained popularity in recent years. In this work, we demonstrated, for the first time, its power to fully elucidate the 2-dimensional and 3-dimensional structures of two epimers in an epimeric mixture of 6-hydroxyhippeastidine. DFT calculation of chemical shifts was first performed to assist the assignment of planar structures. Furthermore, relative and absolute configurations were established by three different ways of computer-assisted structure elucidation (CASE) coupled with ORD/ECD/VCD spectroscopies. In addition, the significant added value of OR/ORD computations to relative and absolute configuration determination was also revealed. Remarkably, the differentiation of two enantiomeric scaffolds (crinine and haemanthamine) was accomplished via OR/ORD calculations with cross-validation by ECD and VCD.
Active Loading of Pectin Hydrogels for Targeted Drug Delivery

Hydrogels provide a promising method for the targeted delivery of protein drugs. Loading the protein drug into the hydrogel free volume can be challenging due to limited quantities of the drug (e.g., growth factor) and complex physicochemical properties of the hydrogel. Here, we investigated both passive and active loading of the heteropolysaccharide hydrogel pectin. Passive loading of glass phase pectin films was evaluated by contact angles and fractional thickness of the pectin films. Four pectin sources demonstrated mean contact angles of 88° with water and 122° with pleural fluid (p < 0.05). Slow kinetics and evaporative losses precluded passive loading. In contrast, active loading of the translucent pectin films was evaluated with the colorimetric tracer methylene blue. Active loading parameters were systematically varied and recorded at 500 points/s. The distribution of the tracer was evaluated by image morphometry. Active loading of the tracer into the pectin films required the optimization of probe velocity, compression force, and contact time. We conclude that active loading using pectin-specific conditions is required for the efficient embedding of low viscosity liquids into pectin hydrogels.
RR Lyrae stars as probes of the outer Galactic halo: Chemical and kinematic analysis of a pilot sample

We report the spectroscopic analysis of 20 halo ab-type RR Lyrae stars with heliocentric distances between 15 and 165 kpc, conducted using medium-resolution spectra from the Magellan Inamori Kyocera Echelle (MIKE) spectrograph. We obtain the systemic line-of-sight velocities of our targets with typical uncertainties of 5–10 km s−1, and compute orbital parameters for a subsample out to 50 kpc from the Galactic centre, including proper motion data from Gaia DR3. The orientation of our stars’ orbits, determined for an isolated Milky Way and for a model perturbed by the Large Magellanic Cloud, appears to suggest an accreted origin for at least half of the sample. In addition, we derive atmospheric parameters and chemical abundance ratios for seven stars beyond 20 kpc. The derived α-abundances of five of these stars follow a Milky Way halo-like trend, while the other two display an underabundance of α-elements for their [Fe/H], indicating an association with accretion events. Furthermore, based on the [Sr/Ba] ratio, we can speculate about the conditions for the formation of a potential chemically peculiar carbon-enhanced metal-poor (CEMP) RR Lyrae star. By analysing the stars’ orbital parameters and abundance ratios, we find hints of association of two of our stars with two massive satellites, namely the Large Magellanic Cloud and Sagittarius. Overall, our results are in line with the suggestion that the accretion of sub-haloes largely contributes to the outer halo stellar populations.
Bali Quality and Sustainable Tourism Development towards Local Economic Impact

Quality and sustainable tourism is one of the drivers in the new economic sector, creating attractions and superior products that are of interest to the international market and world buyers. This study aims at investigating tourism development efforts through the annual execution of Bali Beyond Travel Fair (BBTF) and Bali Arts Festival (BAF) which will have an economic impact on local communities and tourism actors. This study uses an interpretative qualitative analysis model through observational data collection, in-depth interviews and document studies. The results of the study show that tourism activities create demand, both consumption and investment which in turn will lead to goods and services production activities in improving people's welfare and economic growth, especially the multiplier effect for local communities. Therefore, the quality and sustainable tourism that is carried out regularly at BBTF and BAF is one of the drivers in the new economic sector, creating superior attractions and products that are of interest to the international market and world buyers.
Freehand Pre-drilling Technique for Lateral Mass Screw Fixation Is More Efficient and Reliable Versus Sequential Drilling Technique: A Sawbone Analysis

Background Since posterior cervical fixation with lateral mass screws was introduced in 1979, multiple techniques have been described in the literature. However, no study to date has determined whether pre-drilling all lateral masses prior to screw insertion has a benefit over the traditional sequential drilling and screw insertion on the alignment of the screw-rod construct. This study sought to determine the efficacy and efficiency in achieving alignment with a novel pre-drilling technique compared to the traditional sequential drilling technique. The authors hypothesized that the novel pre-drilling technique could be applied more quickly and precisely than the traditional sequential drilling technique. Methods Eight cervical spine sawbones models were utilized to place 64 lateral mass screws by two surgeons. The pre-drilling technique was utilized to place 32 screws in four models, and the sequential drilling technique was utilized to place the 32 screws in the remaining four models. In the traditional sequential drilling technique, each lateral mass underwent screw tract preparation and insertion before proceeding to the subsequent vertebra. In the pre-drilling technique, all lateral masses were marked and drilled sequentially before screw placement. CT imaging with 3D reconstructions was generated for all models. Variability in screw placement and time taken to fully instrument the models were compared. Results The mean time to completion of the pre-drilling technique was 337 ± 22 seconds compared to 490 ± 22 seconds with the traditional technique (p<0.01). There was a significantly higher variability in the coronal plane within the traditional group between C5 and C6 compared to other adjacent vertebrae (p<0.05). There was no significant difference in the start point variability and the overall tightness of line fit between the techniques. Conclusions Our study suggests that a novel pre-drilling technique for lateral mass screw insertion may be more efficient and reliable than the traditional sequential drilling technique. In addition, this technique may reduce the need for rod contouring or additional implants to optimize the alignment of cervical instrumentation. However, further clinical studies are necessary to validate the potential clinical and radiologic benefits of this described technique.