Miracle-dz/UniFAR-FTUs huggingface dataset:
A COMPARATIVE EXPRESSIONAL ANALYSIS OF A FAMILY OF CCA-LIKE MYB TRANSCRIPTION FACTORS IN TWO HIGHER PLANT SPECIES

3 To my parents with love and respect 4 ACKNOWLEDGMENTS Most of all, I thank my family for their endless love and support. They are the pillars of my strength. I also thank my advisor, Dr. David Oppenheimer for affording me the opportunity to attend the University of Florida. I also express gratitude to Dr. Bernard Hauser for his support of this work. A special thanks to Zhengui Zheng for his time and assistance with this project. Also I thank Xiaoguo Zhang and Stacey Jeffries for the invaluable advice they offered and the support they provided me throughout my time at UF. Finally I extend special appreciation to the love of my life, Brad for his continued encouragement. Circadian clocks are ubiquitous among most living species. Since life on Earth originated in the presence of light/dark cycles, organisms had to evolve mechanisms to cope with such environmental fluctuations. An accurate timekeeping apparatus affords an organism with temporal organization of crucial molecular and cellular processes. The components that constitute the central oscillator of the clock vary greatly among plants and animals but the basic architecture appears similar. This resemblance serves as a foundation on which evolutionary-based investigations on the conservation of such machinery can be conducted. In the plant Arabidopsis thaliana, two genes involved in circadian regulation were identified and characterized as members of a family of Myb transcription factors characterized by only one Myb repeat sequence. These genes, CCA1 and LHY, are necessary to maintain rhythmicity in the plant and have been shown to have a role in floral induction. Using the Floral Genome Project (FGP) database of known flowering genes, two EST homologs of CCA1 and LHY were identified in Eschscholzia californica, eca_4_183384 and eca_4_184056 (E1 and E2), based on sequence similarity. These orthologs demonstrate transcript oscillations over a 24hr period with peak levels of expression occurring just prior to dawn. In situ analyses revealed similar patterns of expression in young and older floral tissue of both plant species. In this paper I present 8 molecular evidence that the transcription-translation-based feedback loop mechanism of the circadian oscillator is conserved in these higher plant species and suggest that this mechanism can also be observe din other higher plants. Biological Rhythms The daily rotation of the earth leads to periodic fluctuations in environmental conditions. Because life on Earth originated in the presence of a cyclical environment, many organisms have evolved timing …
Stimulation of Synaptic Vesicle Exocytosis by the Mental Disease Gene DISC1 is Mediated by N-Type Voltage-Gated Calcium Channels

Lesions and mutations of the DISC1 (Disrupted-in-schizophrenia-1) gene have been linked to major depression, schizophrenia, bipolar disorder and autism, but the influence of DISC1 on synaptic transmission remains poorly understood. Using two independent genetic approaches—RNAi and a DISC1 KO mouse—we examined the impact of DISC1 on the synaptic vesicle (SV) cycle by population imaging of the synaptic tracer vGpH in hippocampal neurons. DISC1 loss-of-function resulted in a marked decrease in SV exocytic rates during neuronal stimulation and was associated with reduced Ca2+ transients at nerve terminals. Impaired SV release was efficiently rescued by elevation of extracellular Ca2+, hinting at a link between DISC1 and voltage-gated Ca2+ channels. Accordingly, blockade of N-type Cav2.2 channels mimics and occludes the effect of DISC1 inactivation on SV exocytosis, and overexpression of DISC1 in a heterologous system increases Cav2.2 currents. Collectively, these results show that DISC1-dependent enhancement of SV exocytosis is mediated by Cav2.2 and point to aberrant glutamate release as a probable endophenotype of major psychiatric disorders.
Path planning and optimization in the traveling salesman problem: Nearest neighbor vs. region-based strategies

According to the number of targets, route planning can be a very complex task. Human navigators, however, usually solve route planning tasks fastly and efficiently. Here two experiments are presented that studied human route planning performance, route planning strategies employed, and cognitive processes involved. For this, 25 places were arranged on a regular grid in a large room. Each place was marked by a unique symbol. Subjects were repeatedly asked to solve traveling salesman problems (TSP), i.e. to find the shortest closed loop connecting a given start place with a number of target places. For this, subjects were given a so-called 'shopping list' depicting the symbols of the start place and the target places. While the TSP is computationally hard, sufficient solutions can be found by simple strategies such as the nearest neighbor strategy. In Experiment 1, it was tested whether humans deployed the nearest neighbor strategy (NNS) when solving the TSP. Results showed that subjects outperformed the NNS in cases in which the NNS did not predict the optimal solution, suggesting that the NNS is not sufficient to explain human route planning behavior. As a second possible strategy a region-based approach was tested in Experiment 2. When optimal routes required more region transitions than other, sub-optimal routes, subjects preferred these sub-optimal routes. This result suggests that subjects first planned a coarse route on the region level and then refined the route during navigation. Such a hierarchical planning stragey would allow to reduce computational effort during route planning. In a control condition, the target places were directly marked in the environment rather than being depicted on the shopping list. As subjects did not have to identify and remember the positions of the target places based on the shopping list during route planning, this control condition tested for the influence of spatial working memory for route planning performance. Results showed a strong performance increase in the control condition, emphasizing the prominent role of spatial working memory for route planning.
TCP over ad hoc networks: interaction with power control and IEEE 802.11

In this paper we evaluate how the TCP behaves in a completely distributed ad hoc network, with particular attention to its energy behavior. In particular we analyze the interactions between TCP and the Distributed Power Control (DPC) technique [1] (a mechanisms able to achieve energy efficiency), and between TCP and the IEEE 802.11 MAC protocol. The performance have been obtained by means of simulation, by referring to the Dijkstra, Link State and AODV routing protocols.
A Fixed Point Iterative Method for Low $n$-Rank Tensor Pursuit

The linearly constrained tensor <formula formulatype="inline"><tex Notation="TeX">$n$</tex> </formula>-rank minimization problem is an extension of matrix rank minimization. It is applicable in many fields which use the multi-way data, such as data mining, machine learning and computer vision. In this paper, we adapt operator splitting technique and convex relaxation technique to transform the original problem into a convex, unconstrained optimization problem and propose a fixed point iterative method to solve it. We also prove the convergence of the method under some assumptions. By using a continuation technique, we propose a fast and robust algorithm for solving the tensor completion problem, which is called FP-LRTC (Fixed Point for Low <formula formulatype="inline"><tex Notation="TeX">$n$</tex> </formula>-Rank Tensor Completion). Our numerical results on randomly generated and real tensor completion problems demonstrate that this algorithm is effective, especially for “easy” problems.
HIV-1 Neutralization Profile and Plant-Based Recombinant Expression of Actinohivin, an Env Glycan-Specific Lectin Devoid of T-Cell Mitogenic Activity

The development of a topical microbicide blocking the sexual transmission of HIV-1 is urgently needed to control the global HIV/AIDS pandemic. The actinomycete-derived lectin actinohivin (AH) is highly specific to a cluster of high-mannose-type glycans uniquely found on the viral envelope (Env). Here, we evaluated AH's candidacy toward a microbicide in terms of in vitro anti-HIV-1 activity, potential side effects, and recombinant producibility. Two validated assay systems based on human peripheral blood mononuclear cell (hPBMC) infection with primary isolates and TZM-bl cell infection with Env-pseudotyped viruses were employed to characterize AH's anti-HIV-1 activity. In hPMBCs, AH exhibited nanomolar neutralizing activity against primary viruses with diverse cellular tropisms, but did not cause mitogenicity or cytotoxicity that are often associated with other anti-HIV lectins. In the TZM-bl-based assay, AH showed broad anti-HIV-1 activity against clinically-relevant, mucosally transmitting strains of clades B and C. By contrast, clade A viruses showed strong resistance to AH. Correlation analysis suggested that HIV-1′s AH susceptibility is significantly linked to the N-glycans at the Env C2 and V4 regions. For recombinant (r)AH expression, we evaluated a tobacco mosaic virus-based system in Nicotiana benthamiana plants as a means to facilitate molecular engineering and cost-effective mass production. Biochemical analysis and an Env-mediated syncytium formation assay demonstrated high-level expression of functional rAH within six days. Taken together, our study revealed AH's cross-clade anti-HIV-1 activity, apparent lack of side effects common to lectins, and robust producibility using plant biotechnology. These findings justify further efforts to develop rAH toward a candidate HIV-1 microbicide.
The Association between Stress, Anxiety, and Low Birth Weights among Infants at Hamilton Community Health Network

The prevalence of low birth weight (LBW) infants in Flint, MI is higher than the national average. Although LBW infants are at an increased risk for the subsequent development of health problems in childhood, few studies have examined the factors associated with the occurrence ofLBW infants. The purpose of this study was to examine whether the perceived stress among mothers was associated with LBW infant-rates. A cross-sectional study using a self-administered survey was conducted at Hamilton Community Health Network in 2017. The eligibility criteria included: aged between 18 and 45 years, English speakers, given birth in the past 24 months, and obtained prenatal care services during pregnancy. Descriptive statistics, chi square-test and analysis of variances were used to compare characteristics in eligible mothers. Of 157 eligible mothers, their mean age was 26 years. A majority were Mrican American (76%), single (67%), obese (BMI=32.4), GED or less (67%), and had Medicaid (92%). Results indicated that there was an association between stress and anxiety among mothers at Hamilton Community Health Network. Mothers with LBW infants were more likely to be African American, obese and have Medicaid, although this was not statistically significant . To summarize, the perceived stress may influence the prevalence ofLBW infants in Flint, MI. Further studies are needed to identify possible psycho-social mediators to improve pregnancy outcomes among mothers living in the area. Running Head: STRESS, ANXIETY, AND LOW BIRTH WEIGHTS 4
Protein and Amino Acid Requirements for Poultry

Introduction This fact sheet has been developed to support the implementation of the Natural Resources Conservation Service Feed Management 592 Practice Standard. The Feed Management 592 Practice Standard was adopted by NRCS in 2003 as another tool to assist with addressing resource concerns on livestock and poultry operations. Feed management can assist with reducing the import of nutrients to the farm and reduce the excretion of nutrients in manure.
Matching conditions and Higgs mass upper bounds revisited

Matching conditions relate couplings to particle masses. We discuss the importance of one-loop matching conditions in the Higgs boson and top quark sector as well as the choice of the matching scale. We argue for matching scales {mu}{sub 0,t}{approx_equal}m{sub t} and {mu}{sub 0,H}{approx_equal}max{l_brace}m{sub t},M{sub H}{r_brace}. Using these results, the two-loop Higgs boson mass upper bounds are reanalyzed. Previous results for {Lambda}{approx} few TeV are found to be too stringent, and a recent update is found to be wrong. For {Lambda}=10{sup 19} GeV we find M{sub H}{lt}180{plus_minus}4{plus_minus}5 GeV, the first error indicating the theoretical uncertainty and the second error reflecting the experimental uncertainty due to m{sub t}=175{plus_minus}6 GeV. Hence a Higgs boson mass of about 160{endash}170 GeV certainly satisfies both upper and lower Higgs boson mass bounds for cutoff scales up to {Lambda}=10{sup 19} GeV if m{sub t}=175 GeV. For such Higgs boson and top quark masses the renormalization group behavior of the minimal standard model does not require new physics to set in before the Planck scale. {copyright} {ital 1997} {ital The American Physical Society}
Antioscillatory Effects of Nociceptin/Orphanin FQ in Synaptic Networks of the Rat Thalamus

Postsynaptic and presynaptic effects of nociceptin/orphanin FQ (N/OFQ), the endogenous ligand of the opioid-like orphan receptor, were investigated in an in vitro slice preparation of the rat thalamic reticular nucleus (NRT) and ventrobasal complex (VB). In NRT as well as VB, all tested neurons developed an outward current on application of 1 μm N/OFQ. Basic properties of the N/OFQ-induced current included inward rectification, dependence on extracellular K+, reduction by 100 μm Ba+, antagonistic effect of [Nphe1]nociceptin(1–13)NH2, and sensitivity to internal GDP-β-S. Miniature IPSCs (mIPSCs) mediated by GABAA receptors in VB neurons were not affected by 1 μm N/OFQ. In addition, paired-pulse depression of evoked IPSCs was unchanged, indicating a lack of presynaptic effects. By comparison, N/OFQ application resulted in a reduction in frequency of miniature EPSCs (mEPSCs) in a subpopulation of NRT neurons, whereas paired-pulse facilitation of evoked EPSCs was not altered. In either nucleus, current-clamp experiments revealed a hyperpolarization and associated decrease in input resistance in response to N/OFQ. Although N/OFQ had no measurable effect on calcium-mediated burst activity evoked by depolarizing steps from hyperpolarized values of the membrane potential, rebound bursts on relief of hyperpolarizing current steps were decreased. Slow thalamic oscillations induced in vitro by extracellular stimulation were dampened by N/OFQ in VB and NRT, as seen by delayed onset of rhythmic multiple-unit activity and reduction in amplitude and duration. We conclude that N/OFQ reduces the excitability of NRT and VB neurons predominantly through an increase of a G-protein-coupled inwardly rectifying K+ conductance.
Hierarchical Support Vector Machines for Audio Classification

Audio data is one of typical multimedia data and it contains plenty of information. Audio retrieval is becoming important content in multimedia information retrieval. In multimedia retrieval researches, it becomes more and more important research part how to construct better classifiers for audio classification and retrieval. Support Vector Machines, a novel method of the Pattern Recognition, presents excellent performance in solving the problems with small sample, nonlinear and local minima. But audio classification is a multi-class classification problem and it's just one of problems to be solved in SVM researches. In this paper, it compares several common Support Vector Machines and proposes a hierarchical Support Vector Machines based on audio features cluster method, combining audio features and hierarchical SVMS. It uses hierarchical classification method to classify audio data and it's proved better performance by experiments.
Use of the Patient Assessment of Chronic Illness Care (PACIC) with diabetic patients: relationship to patient characteristics, receipt of care, and self-management.

OBJECTIVE There is a dearth of information on the extent to which diabetic patients receive care congruent with the chronic care model (CCM) and evidence-based behavioral counseling. This study evaluates a new instrument to fill this gap. RESEARCH DESIGN AND METHODS A heterogeneous sample of 363 type 2 diabetic patients completed the original Patient Assessment of Chronic Illness Care (PACIC), along with additional items that allowed it to be scored according to the "5As" (ask, advise, agree, assist, and arrange) model of behavioral counseling. We evaluated relationships between survey scores and patient characteristics, quality of diabetes care, and self-management. RESULTS Findings replicated those of the initial PACIC validation study but with a much larger sample of diabetic patients and more Latinos. Areas of CCM activities reported least often were goal setting/intervention tailoring and follow-up/coordination. The 5As scoring revealed that patients were least likely to receive assistance with problem solving and arrangement of follow-up support. Few demographic or medical characteristics were related to PACIC or 5As scores, but survey scores were significantly related to quality of diabetes care received and level of physical activity. CONCLUSIONS The PACIC and the new 5As scoring method appear useful for diabetic patients. Its use is encouraged in future research and quality improvement studies.
Steel-Sense: Integrating Machine Elements with Sensors by Additive Manufacturing

Many interactive devices use both machine elements and sensors, simultaneously but redundantly enabling and measuring the same physical function. We present Steel-Sense, an approach to joining these two families of elements to create a new type of HCI design primitive. We leverage recent developments in 3D printing to embed sensing in metal structures that are otherwise difficult to equip with sensors, and present four design principles, implementing (1) an electronic switch integrated within a ball bearing; (2) a voltage divider within a gear; (3) a variable capacitor embedded in a hinge; and (4) a pressure sensor within a screw. Each design demonstrates a different sensing principle, and signals its performance through (1) movement; (2) position; (3) angle (4) or stress. We mirror our elements physical performance in a virtual environment, evaluate our designs electronically and structurally, and discuss future work and implications for HCI research.
Planning and Implementing Trajectories for Autonomous Underwater Vehicles to Track Evolving Ocean Processes Based on Predictions from a Regional Ocean Model

Path planning and trajectory design for autonomous underwater vehicles (AUVs) is of great importance to the oceanographic research community because automated data collection is becoming more prevalent. Intelligent planning is required to maneuver a vehicle to high-valued locations to perform data collection. In this paper, we present algorithms that determine paths for AUVs to track evolving features of interest in the ocean by considering the output of predictive ocean models. While traversing the computed path, the vehicle provides near-real-time, in situ measurements back to the model, with the intent to increase the skill of future predictions in the local region. The results presented here extend preliminary developments of the path planning portion of an end-to-end autonomous prediction and tasking system for aquatic, mobile sensor networks. This extension is the incorporation of multiple vehicles to track the centroid and the boundary of the extent of a feature of interest. Similar algorithms to those presented here are under development to consider additional locations for multiple types of features. The primary focus here is on algorithm development utilizing model predictions to assist in solving the motion planning problem of steering an AUV to high-valued locations, with respect to the data desired. We discuss the design technique to generate the paths, present simulation results and provide experimental data from field deployments for tracking dynamic features by use of an AUV in the Southern California coastal ocean.
FEASIBIITY AND EFFICACY OF A MOVEMENT-TRAINING PROGRAM ON PHYSICAL FITNESS, FUNDAMENTAL MOVEMENT SKILLS, AND PHYSICAL ACTIVITY IN THIRD AND FOURTH GRADE STUDENTS

BACKGROUND: The purpose of this study was to systematically assess the feasibility and acceptability of a teacher-led, PE-based, fitness program in third and fourth grade students using a mixed-methods approach. METHODS: Participating classrooms (n=7) were randomized (by classroom) into two groups: intervention (n=4; INT) or control (n=3; CON). All students in the INT group classrooms participated in a 12-week movement-training intervention. Process evaluation data (i.e. fidelity, dose, reach, and satisfaction) were collected throughout the 12weeks in all INT and CON classrooms. RESULTS: Our results indicated that 46 of the 68 sessions (67%) had all three components of the intervention (15 minutes, fun atmosphere, high intensity). During our study, the intervention was delivered within 15-minutes during 59 of the 68 total intervention sessions (87%). Research staff indicated the FIT sessions were conducted in a fun atmosphere 65 out of the 68 total intervention sessions (96%). The intervention classrooms received the FIT intervention an average of 17 times out of a possible 22 planned sessions (78%). The boys and girls who participated in the FIT intervention reported that they enjoyed the 12week intervention. When asked what was most enjoyable, both groups reported the aspects they found most enjoyable to be the equipment used at the stations (i.e. spooner board) and circuit-like style of the stations 41 around the gym. The teacher expressed the desire to continue delivering the intervention after the study ended. CONCLUSIONS: This study is the first to report on the feasibility and acceptability of delivering a FIT intervention in elementary school-aged children. Overall, the FIT framework was successfully implemented and well-accepted by the students and PE teacher. The feasibility and efficacy of the FIT intervention modality should continue to be tested in larger, more diverse populations over longer durations.
The Educational Asset Market: A Finance Perspective on Human Capital Investment ♣

Like the stock market, the human capital market consists of a wide range of assets, i.e. educations. Each young individual chooses the educational asset that matches his preferred combination of risk and return in terms of future income. A unique register-based data set with exact information on type and level of education enables us to focus on the shared features between human capital and stock investments. An innovative finance-labor approach is applied to study the educational asset market. A risk-return trade-off is revealed which is not directly related to the length of education.