Miracle-dz/UniFAR-FTUs huggingface dataset:
Pathogenic Yeasts Cryptococcus neoformans and Candida albicans Produce Immunomodulatory Prostaglandins

ABSTRACT Enhanced prostaglandin production during fungal infection could be an important factor in promoting fungal colonization and chronic infection. Host cells are one source of prostaglandins; however, another potential source of prostaglandins is the fungal pathogen itself. Our objective was to determine if the pathogenic yeastsCryptococcus neoformans and Candida albicansproduce prostaglandins and, if so, to begin to define the role of these bioactive lipids in yeast biology and disease pathogenesis. C. neoformans and C. albicans both secreted prostaglandins de novo or via conversion of exogenous arachidonic acid. Treatment with cyclooxygenase inhibitors dramatically reduced the viability of the yeast and the production of prostaglandins, suggesting that an essential cyclooxygenase like enzyme may be responsible for fungal prostaglandin production. A PGE series lipid was purified from both C. albicans and C. neoformans and was biologically active on both fungal and mammalian cells. Fungal PGEx and synthetic PGE2 enhanced the yeast-to-hypha transition in C. albicans. Furthermore, in mammalian cells, fungal PGEx down-modulated chemokine production, tumor necrosis factor alpha production, and splenocyte proliferation while up-regulating interleukin 10 production. These are all activities previously documented for mammalian PGE2. Thus, eicosanoids are produced by pathogenic fungi, are critical for growth of the fungi, and can modulate host immune functions. The discovery that pathogenic fungi produce and respond to immunomodulatory eicosanoids reveals a virulence mechanism that has potentially great implications for understanding the mechanisms of chronic fungal infection, immune deviation, and fungi as disease cofactors.
Inhibition of oxidative drug metabolism by orphenadrine: in vitro and in vivo evidence for isozyme-specific complexation of cytochrome P-450 and inhibition kinetics.

The anti-parkinsonian agent orphenadrine has been shown to form an in vitro metabolic intermediate (MI) complex in hepatic microsomes isolated from phenobarbital (PB)-treated rats. The present study was undertaken to assess the cytochrome P-450 isozyme specificity of inhibition and MI complexation. Spectral studies with untreated and PB-induced rat hepatic microsomes confirmed earlier reports on the selectivity of P-450 complexation by orphenadrine; MI complex formation was only observed with PB-induced microsomes. Inhibition studies with the P-450 substrates androst-4-ene-3,17-dione (androstenedione) and 7-pentoxyresorufin revealed selective inhibition of P-450 PB-B/D-associated monooxygenase activity. Thus, in microsomes from untreated male rats, orphenadrine failed to significantly inhibit (less than 50% inhibition up to a concentration of 300 microM) any of the major pathways of P-450-associated androstenedione metabolism. Preincubation of these microsomal fractions with orphenadrine and NADPH was not associated with increased inhibition of androstenedione metabolism. However, in PB-induced microsomes, P-450 PB-B/D-specific androstenedione 16 beta-hydroxylase activity was significantly and selectively inhibited (IC50 = 90 microM). Preincubation of orphenadrine with NADPH-supplemented PB-induced microsomes for 2, 4, or 8 min before androstenedione addition resulted in increased inhibition toward 16 beta-hydroxylase activity, lowering the observed IC50 to 6.6, 0.47, and 0.06 microM), respectively. Preincubation did not affect the selectivity of inhibition. In the absence of preincubation, orphenadrine appeared to be a potent mixed (competitive/noncompetitive)-type inhibitor of P-450 PB-B/D-associated pentoxyresorufin O-depentylation (Ki = 3.8 microM). Preincubation of orphenadrine with NADPH-supplemented microsomal fractions for 4 min resulted in a 30-fold lowering of the apparent inhibitor constant (Ki = 0.13 microM) and a change in the apparent inhibition kinetics to noncompetitive. Treatment of rats with orphenadrine (75 mg/kg/day intraperitoneally for 3 days) was associated with a 2-fold induction of total hepatic P-450, a 5- and 2.4-fold induction of androstenedione 16 beta- and 6 beta-hydroxylase activity, respectively, and formation of an orphenadrine-P-450 MI complex. Western blots of orphenadrine-induced microsomes revealed a 20-fold increase in P-450 PB-B/D-immunoreactive protein. (ABSTRACT TRUNCATED AT 400 WORDS)
E-motional advantage: performance and satisfaction gains with affective computing

Emotions are now recognized as complex human control systems, crucial to decision making, creativity, playing and learning. Affective technologies may offer improved interaction and commercial promise. In the past, research has focused on technical development work, leaving many questions about user preferences unanswered. For this user-centered study, 60 participants played a simple 'word ladder' game under different controlled conditions. Using 2 x 2 factorial design, and a Wizard of Oz scenario, half the participants interacted with a system that adapted on the basis of the user's emotional expression and half were told the system could react to their emotional expressions. We established that when using an apparently affective system, users perform significantly better and report themselves as feeling significantly happier. We also discuss behavioral responses to the different conditions. These results are relevant to the design of future affective systems.
Segment Level Authentication: Combating internet source spoofing

This paper presents SLA (Segment Level Authentication), a transport segment level solution designed to prevent both of the intra-domain and inter-domain source spoofing. SLA is based on public key cryptography authentication. It enables intermediate network nodes the ability to validate the packet authenticity by verifying authentication information carried in packets. Although public key cryptography is computationally intensive and induces the traffic overhead, SLA leverages FPGA (Field Programmable Gate Array) based ECC (Elliptic Curve Cryptography) hardware cryptography accelerator to decrease the computation and traffic overhead. SLA provides incremental deployment and offers incentives for both of hosts and ASes. We find that the SLA is feasible for Gigabit links and can effectively mitigate source spoofing in both of intra-domain and inter-domain networks.
Positional Quality Assessment of Orthophotos Obtained from Sensors Onboard Multi-Rotor UAV Platforms

In this study we explored the positional quality of orthophotos obtained by an unmanned aerial vehicle (UAV). A multi-rotor UAV was used to obtain images using a vertically mounted digital camera. The flight was processed taking into account the photogrammetry workflow: perform the aerial triangulation, generate a digital surface model, orthorectify individual images and finally obtain a mosaic image or final orthophoto. The UAV orthophotos were assessed with various spatial quality tests used by national mapping agencies (NMAs). Results showed that the orthophotos satisfactorily passed the spatial quality tests and are therefore a useful tool for NMAs in their production flowchart.
A Biomimetic Approach Based on Immune Systems for Classification of Unstructured Data

In this paper we present the results of unstructured data clustering in this case a textual data from Reuters 21578 corpus with a new biomimetic approach using immune system. Before experimenting our immune system, we digitalized textual data by the n-grams approach. The novelty lies on hybridization of n-grams and immune systems for clustering. The experimental results show that the recommended ideas are promising and prove that this method can solve the text clustering problem.
Modeling Discrete Choices in the Presence of Inertia and Serial Correlation

The concept of habit or inertia in the context of (reluctance to) change in travel behavior has an important bearing on transport policy (e.g., how to break car use habits) and has remained an unresolved issue in demand modeling. Another major problem in modeling the response to policy measures is the potential correlation or dependence between the choices made by a given individual over time (i.e., serial correlation). The two phenomena are closely related. This paper discusses the effects of considering inertia and serial correlation on travel choices. We formulate a fairly general discrete choice model that incorporates randomly distributed inertia thresholds and allow for serial correlation. The inertia thresholds may also be a function of an individual's socioeconomic characteristics and choice conditions. The model can be applied with panel data as well as with mixed revealed and stated preference data. We applied it to real and simulated data, confirming that if these phenomena exist in the population but are not considered, serious errors in model estimation and prediction may arise, especially in the case of large policy impacts.
Isoflavonoid-inducible resistance to the phytoalexin glyceollin in soybean rhizobia

The antibacterial effect of the soybean phytoalexin glyceollin was assayed using a liquid microculture technique. Log-phase cells of Bradyrhizobium japonicum and Sinorhizobium fredii were sensitive to glyceollin. As revealed by growth rates and survival tests, these species were able to tolerate glyceollin after adaptation. Incubation in low concentrations of the isoflavones genistein and daidzein induced resistance to potentially bactericidal concentrations of glyceollin. This inducible resistance is not due to degradation or detoxification of the phytoalexin. The inducible resistance could be detected in B. japonicum 110spc4 and 61A101, representing the two taxonomically divergent groups of this species, as well as in S. fredii HH103, suggesting that this trait is a feature of all soybean-nodulating rhizobia. Glyceollin resistance was also inducible in a nodD1D2YABC deletion mutant of B. japonicum 110spc4, suggesting that there exists another recognition site for flavonoids besides the nodD genes identified so far. Exudate preparations from roots infected with Phytophthora megasperma f. sp. glycinea exhibited a strong bactericidal effect toward glyceollin-sensitive cells of B. japonicum. This killing effect was not solely due to glyceollin since purified glyceollin at concentrations similar to those present in exudate preparations had a much lower toxicity. However, glyceollin-resistant cells were also more resistant to exudate preparations than glyceollin-sensitive cells. Isoflavonoid-inducible resistance must therefore be ascribed an important role for survival of rhizobia in the rhizosphere of soybean roots.
The use of radiographic contrast media during PCI: A focused review

Lloyd W. Klein,* MD, FSCAI, Mark W. Sheldon, MD, FSCAI, Jeffrey Brinker, MD, FSCAI, Timothy A. Mixon, MD, FSCAI, Kimberly Skelding, MD, FSCAI, Adam O. Strunk, MD, FSCAI, Carl L. Tommaso, MD, FSCAI, Bonnie Weiner, MD, FSCAI, Steven R. Bailey, MD, FSCAI, Barry Uretsky, MD, FSCAI, Morton Kern, MD, FSCAI, Warren Laskey, MD, FSCAI, and On Behalf of the Interventional Committee of the Society for Cardiovascular Angiography and Interventions
Molecular brakes regulating mTORC1 activation in skeletal muscle following synergist ablation

The goal of the current work was to profile positive (mTORC1 activation, autocrine/paracrine growth factors) and negative [AMPK, unfolded protein response (UPR)] pathways that might regulate overload-induced mTORC1 (mTOR complex 1) activation with the hypothesis that a number of negative regulators of mTORC1 will be engaged during a supraphysiological model of hypertrophy. To achieve this, mTORC1-IRS-1/2 signaling, BiP/CHOP/IRE1α, and AMPK activation were determined in rat plantaris muscle following synergist ablation (SA). SA resulted in significant increases in muscle mass of ∼4% per day throughout the 21 days of the experiment. The expression of the insulin-like growth factors (IGF) were high throughout the 21st day of overload. However, IGF signaling was limited, since IRS-1 and -2 were undetectable in the overloaded muscle from day 3 to day 9. The decreases in IRS-1/2 protein were paralleled by increases in GRB10 Ser501/503 and S6K1 Thr389 phosphorylation, two mTORC1 targets that can destabilize IRS proteins. PKB Ser473 phosphorylation was higher from 3–6 days, and this was associated with increased TSC2 Thr939 phosphorylation. The phosphorylation of TSC2 Thr1345 (an AMPK site) was also elevated, whereas phosphorylation at the other PKB site, Thr1462, was unchanged at 6 days. In agreement with the phosphorylation of Thr1345, SA led to activation of AMPKα1 during the initial growth phase, lasting the first 9 days before returning to baseline by day 12. The UPR markers CHOP and BiP were elevated over the first 12 days following ablation, whereas IRE1α levels decreased. These data suggest that during supraphysiological muscle loading at least three potential molecular brakes engage to downregulate mTORC1.
Prevalence and severity of patient harm in a sample of UK-hospitalised children detected by the Paediatric Trigger Tool

The measurement and examination of adverse events (AEs) that occur in children during hospital admissions is essential if we are to prevent, reduce or ameliorate the harm experienced. The UK Paediatric Trigger Tool (UKPTT) is a method of retrospective case note review that measures harm in hospitalised children. Objectives To examine the harm resulting from the processes of healthcare in hospitalised children from centres providing data to the National Health Service (NHS) Institute UKPTT data portal, to understand the positive predictive values of triggers and to make recommendations for the further development of the trigger tool. Setting 25 hospitals across the UK, including secondary, tertiary and quaternary paediatric centres. Participants Randomly selected children who were admitted to hospital for longer than 24 h. Outcome measures The primary outcome measure was the rate of harm (the percentage of children experiencing one or more AEs during a hospital admission). Secondary measures were the severity of harm and performance of triggers. Results Data from 3992 patient admissions were reviewed across the hospitals and submitted to the trigger tool portal from February 2008 to November 2011. At least one AE was reported for 567 (14.2%) patients, with 211 (5.3%) experiencing more than one event. There were 1001 AEs identified. Where harm occurred, it was considered temporary for 923 (92.2%) AEs; however, 43 (4.3%) AEs resulted in the need for life-sustaining interventions, 18 (1.8%) AEs led to permanent harm and for 17 children (1.7% of AEs) the AE was believed to have contributed to death. Conclusions There is a significant, measurable level of harm experienced by children admitted to hospitals in the UK. While most of this harm is temporary, some of it is serious. The UKPTT offers organisations the means to measure and examine the AEs occurring in their hospital in order to reduce harm.
Management of Male and Female Neurogenic Stress Urinary Incontinence in Spinal Cord Injured (SCI) Patients Using Adjustable Continence Therapy

Introduction Artificial urinary sphincter (AUS) is an option for the treatment of neurogenic stress urinary incontinence (nSUI), but complications and re-operation rates are high, and there is no clear indication from guidelines (1). The aim of our study is to evaluate the effectiveness of a less invasive continence device in neurogenic population: Adjustable Continence Therapy ProACT/ACT®. Methods We retrospectively includedpatients with spinal cord injuries in this study, complaining of nSUI and treated at our Institution with Pro-ACT/ACT® implantation. Diagnosis of nSUI was achieved with clinical history data collection and video-urodynamic testing. Results We treated 13 males and 3 females by proACT/ACT device, mean age 47.5 years (range 27-71). Fifteen implantations were performed bilaterally under spinal anesthesia and under fluoroscopic control; in one male patient, only the right balloon was implanted. Mean refilling number was 2.8 (range 0-6), and mean final volume was 3.6 ml. No patient reported any perioperative complications according to Clavien-Dindo. Follow-up was 37 months (range 7-156). Five patients (31%) underwent device explantation for deflate in one case, erosion or migration in three cases (18%), and infection in one case. About 43.75% of patients were dry and 18.75% improved more than 50% their urine loss, 37.5% of patients improved less than 50%, and no one reported worsening of incontinence. Conclusions Implantation of proACT/ACT® device is safe and a minimally invasive procedure also in neurological patients, with a relative low rate of intra and postoperative complications. Efficacy is good, although slightly lower than the results in non-neurological patients.
Design of a Low Power, High Speed Complementary Input Folded Regulated Cascode OTA for a Parallel Pipeline ADC

This paper presents a low-power, high speed complementary input folded regulated cascode operational transconductance amplifier (OTA) designed for the 10bit, 150MSPS parallel pipeline ADC. The OTA plays an important role in the ADC, because of its conversion rate and power consumption are limited by the performance of the OTA. The designed ADC in this paper employs parallel pipeline architecture based on double sampling sampled hold (DSSH) technique, and shares the OTA between two channels of the ADC. The folded cascode OTA consists of fully differential and regulated cascode gain boosting technique. Besides, a Common Mode Feed Back (CMFB) circuit was introduced and some methods are concerned to improve the performance. Then, by proper optimization of the layout design, OTA's mismatch was reduced up-to a great extent. With 1.8V power supply, using the CMOS9T5V 180nm process technology, the simulation shows that the open-loop gain of the OTA is 90.39 dB, the phase margin (PM) is 63.85° with the unity gain bandwidth (UGB) of 700.7 MHz. The power consumption of this OTA is only 3.24 mW, which significantly reduces the whole power consumption of the parallel pipeline ADC.
A Cholesterol Biosynthesis Inhibitor Blocks Staphylococcus aureus Virulence

Staphylococcus aureus produces hospital- and community-acquired infections, with methicillin-resistant S. aureus posing a serious public health threat. The golden carotenoid pigment of S. aureus, staphyloxanthin, promotes resistance to reactive oxygen species and host neutrophil-based killing, and early enzymatic steps in staphyloxanthin production resemble those for cholesterol biosynthesis. We determined the crystal structures of S. aureus dehydrosqualene synthase (CrtM) at 1.58 angstrom resolution, finding structural similarity to human squalene synthase (SQS). We screened nine SQS inhibitors and determined the structures of three, bound to CrtM. One, previously tested for cholesterol-lowering activity in humans, blocked staphyloxanthin biosynthesis in vitro (median inhibitory concentration ∼100 nM), resulting in colorless bacteria with increased susceptibility to killing by human blood and to innate immune clearance in a mouse infection model. This finding represents proof of principle for a virulence factor–based therapy against S. aureus.
Iterative Bayesian Learning for Crowdsourced Regression

Crowdsourcing platforms emerged as popular venues for purchasing human intelligence at low cost for large volume of tasks. As many low-paid workers are prone to give noisy answers, a common practice is to add redundancy by assigning multiple workers to each task and then simply average out these answers. However, to fully harness the wisdom of the crowd, one needs to learn the heterogeneous quality of each worker. We resolve this fundamental challenge in crowdsourced regression tasks, i.e., the answer takes continuous labels, where identifying good or bad workers becomes much more non-trivial compared to a classification setting of discrete labels. In particular, we introduce a Bayesian iterative scheme and show that it provably achieves the optimal mean squared error. Our evaluations on synthetic and real-world datasets support our theoretical results and show the superiority of the proposed scheme.
Fault diagnosis based on deep learning

As representation scheme can severely limit the window by which the system observes its world, deep learning for fault diagnosis is put forward in this paper. It is a real time online scheme that can enhance the accuracy of detection, classification and prediction, and efficient for incipient faults that cannot be detected by traditional statistic technology. A stacked sparse auto encoder is used to learn the deep architectures of fault data to minimize the loss of information. Experiment results show that the proposed method not only improves the divisibility between faults and normal process, but also exhibits a better performance on the accuracy of fault classification for the chemical benchmark, Tennessee Eastman Process (TEP) data.
Genetic epidemiology of hereditary hemorrhagic telangiectasia in a local community in the northern part of Japan

Hereditary hemorrhagic telangiectasia (HHT or Rendu‐Osler‐Weber syndrome) is an autosomal dominant disorder characterized by aberrant vascular development. We report here a genetic epidemiologic study in a county, A, in the Akita prefecture (population 1.2 million) located in northern Japan. Nine HHT patients who had been referred to tertiary‐care hospitals were located in and near the study county. A total of 137 pedigree members were traced of which 81 were alive and 32 were affected by HHT. Complications associated with cerebral or pulmonary arteriovenous malformations were proven in six out of seven families. Linkage analysis in two large families revealed a weak yet suggestive linkage to the HHT1 locus (encoding endoglin; ENG). Three novel mutations were found in four families, all of which led to a frameshift: a G to C transversion at the splicing donor site of intron 3 (Inv3+1 G>C) in one family, one base pair insertion (A) at nucleotide 828 (exon 7) of the endoglin cDNA in two large families (c.828–829 ins A), and a four base pair deletion (AAAG) beginning with nucleotide 1120 (exon 8) of the endoglin cDNA (c.1120–1123 delAAAG) in one family. The insertion of A in exon 11 (c.1470–1471 insA) mutation found in one family has also been reported in a European family. No endoglin gene mutations were found in two families. The population prevalence of HHT in the county was estimated to be 1:8,000∼1:5,000, roughly comparable with those reported in European and U.S. populations, which is contradictory to the traditional view that HHT is rare among Asians. We recommend that families with HHT be screened for gene mutations in order that high‐risk individuals receive early diagnosis and treatment initiation that will substantially alter their clinical course and prognosis. Hum Mutat 19:140–148, 2002. © 2002 Wiley‐Liss, Inc.
Gauge theory: Historical origins and some modern developments

One of the major developments of twentieth-century physics has been the gradual recognition that a common feature of the known fundamental interactions is their gauge structure. In this article the authors review the early history of gauge theory, from Einstein’s theory of gravitation to the appearance of non-Abelian gauge theories in the fifties. The authors also review the early history of dimensional reduction, which played an important role in the development of gauge theory. A description is given of how, in recent times, the ideas of gauge theory and dimensional reduction have emerged naturally in the context of string theory and noncommutative geometry.