Miracle-dz/UniFAR-FTUs huggingface dataset:
Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor

The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and the environment. However, the applications of modern biosensors face numerous problems and require improvement. Some of these problems can be attributed to sensor design, while others can be attributed to technological limitations. The aim of this work is to conduct a theoretical investigation of the “antibody + antigen” complex (AB + AG) detection processes of a SOI-FET biosensor, which may also solve some of the aforementioned problems. Our investigation concentrates on the analysis of the probability of AB + AG complex detection and evaluation. Poisson probability density distribution was used to estimate the probability of the adsorption of the target molecules on the biosensor’s surface and, consequently, to obtain correct detection results. Many implicit and unexpected causes of error detection have been identified for AB + AG complexes using SOI-FET biosensors. We showed that accuracy and time of detection depend on the number of SOI-FET biosensors on a crystal.
A New Buck-Boost Converter Structure With Improved Efficiency

In this paper presents a New Buck-Boost structure with high performance, suitable for practical applications. The performance is evaluated based on direct measurements of voltage and current in the circuit, minimizing errors and producing accurate results. The article compares the proposed Buck-Boost converter with two other converters, the Transformerless Buck-Boost Converter With Positive Output Voltage (Positive Step Buck-Boost - PSBB) and the Negative Output Buck-Boost Converter With Wide Conversion Ratio (Negative Step Buck-Boost - NSBB). The results show that the proposed converter outperforms the other two converters in terms of performance. To validate the proposed design, simulation results obtained using PSIM software are presented in the form of graphs.
Gradual Distance Dispersal Shapes the Genetic Structure in an Alpine Grasshopper

The location of the high mountains of southern Europe has been crucial in the phylogeography of most European species, but how extrinsic (topography of sky islands) and intrinsic features (dispersal dynamics) have interacted to shape the genetic structure in alpine restricted species is still poorly known. Here we investigated the mechanisms explaining the colonisation of Cantabrian sky islands in an endemic flightless grasshopper. We scrutinised the maternal genetic variability and haplotype structure, and we evaluated the fitting of two migration models to understand the extant genetic structure in these populations: Long-distance dispersal (LDD) and gradual distance dispersal (GDD). We found that GDD fits the real data better than the LDD model, with an onset of the expansion matching postglacial expansions after the retreat of the ice sheets. Our findings suggest a scenario with small carrying capacity, migration rates, and population growth rates, being compatible with a slow dispersal process. The gradual expansion process along the Cantabrian sky islands found here seems to be conditioned by the suitability of habitats and the presence of alpine corridors. Our findings shed light on our understanding about how organisms which have adapted to live in alpine habitats with limited dispersal abilities have faced new and suitable environmental conditions.
Development of Antibody-Modified Nanobubbles Using Fc-Region-Binding Polypeptides for Ultrasound Imaging

Ultrasound (US) imaging is a widely used imaging technique. The use of US contrast agents such as microbubbles, which consist of phospholipids and are filled with perfluorocarbon gases, has become an indispensable component of clinical US imaging, while molecular US imaging has recently attracted significant attention in combination with efficient diagnostics. The avidin–biotin interaction method is frequently used to tether antibodies to microbubbles, leading to the development of a molecular targeting US imaging agent. However, avidin still has limitations such as immunogenicity. We previously reported that lipid-based nanobubbles (NBs) containing perfluorocarbon gas are suitable for US imaging and gene delivery. In this paper, we report on the development of a novel antibody modification method for NBs using Fc-region-binding polypeptides derived from protein A/G. First, we prepared anti-CD146 antibody-modified NBs using this polypeptide, resulting in high levels of attachment to human umbilical vein endothelial cells expressing CD146. To examine their targeting ability and US imaging capability, the NBs were administered to tumor-bearing mice. The contrast imaging of antibody-modified NBs was shown to be prolonged compared with that of non-labeled NBs. Thus, this antibody modification method using an Fc-binding polypeptide may be a feasible tool for developing a next-generation antibody-modified US imaging agent.
Towards a Novel Patch Material for Cardiac Applications: Tissue-Specific Extracellular Matrix Introduces Essential Key Features to Decellularized Amniotic Membrane

There is a growing need for scaffold material with tissue-specific bioactivity for use in regenerative medicine, tissue engineering, and for surgical repair of structural defects. We developed a novel composite biomaterial by processing human cardiac extracellular matrix (ECM) into a hydrogel and combining it with cell-free amniotic membrane via a dry-coating procedure. Cardiac biocompatibility and immunogenicity were tested in vitro using human cardiac fibroblasts, epicardial progenitor cells, murine HL-1 cells, and human immune cells derived from buffy coat. Processing of the ECM preserved important matrix proteins as demonstrated by mass spectrometry. ECM coating did not alter the mechanical characteristics of decellularized amniotic membrane but did cause a clear increase in adhesion capacity, cell proliferation and viability. Activated monocytes secreted less pro-inflammatory cytokines, and both macrophage polarization towards the pro-inflammatory M1 type and T cell proliferation were prevented. We conclude that the incorporation of human cardiac ECM hydrogel shifts and enhances the bioactivity of decellularized amniotic membrane, facilitating its use in future cardiac applications.
Analysis of the single stage cycloidal gearbox with lobe defects. Fault diagnosis attempts using coherence function and Morris minimum-bandwidth wavelets

In this article, the frequency characteristics of the forces and torques in the various cycloidal gearbox designs were investigated. The aim of the article is the search for frequency patterns that could be used in the formulation of a fault diagnosis methodology. Numerical analysis was performed in the cycloidal gearbox without defects as well as in cycloidal gearboxes with lobe defects or with removed lobes. The results of the numerical analysis were obtained in the multibody dynamics model of the cycloidal gearbox, implemented in Fortran and using the 2 nd -order Runge-Kutta method for the integration of the motion equations. The used model is planar and uses Hunt and Crossley’s nonlinear contact modelling algorithm, which was modified using the Heaviside function and backlash to fit cycloidal gearbox model convergence demands.Intheanalysisoffaultdiagnosismethods,thecoherencefunctionandMorris minimum-bandwidthwaveletswereused.Itisdifficulttofindauniquepatterninthe resultstouseinthefaultdiagnosisbecauseoftherandomcharacteristicsofthetorques at the input and output shafts. Based on obtained results, a promising, low-vibration cycloidal gearbox design with removed 7 lobes of the single wheel was studied using the FFT algorithm.
ANTISEPTICS RESISTANCE GENES (QACA/B, SMR) DETECTION AND EXPRESSION IN STAPHYLOCOCCUS AUREUS

This study was aimed to investigate the resistance of S. aureus to different antiseptics.This research indicate that the resistance of S.aureus to antiseptics is due to possessing either the smr gene or the qacA/B genes that associated with decreased susceptibility to antiseptics there for this study amid to determine the frequencies of S. aureus chloroxylenol resistant isolates and the presence of the previous genes in these isolates as well as the effect of chloroxylenol on the expression of these genes.189 clinical isolates isolated from skin infections identified as S. aureus in Baghdad by microscopical and biochemical tests. The chloroxylenol resistance S. aureus isolates was identified and chloroxylenol MIC was evaluated for these isolates. Antiseptic resistance genes (qacA/B, smr) were detected by PCR method and the results reveled that 21(84%) out of 25 isolates harbored qacA/B gene. While the smr gene was not demonstrated in any isolates. Furthermore, the chloroxylenol had no effect on qacA/B gene expression in these isolates.
Prophet: An Efficient Feature Indexing Mechanism for Similarity Data Sharing at Network Edge

As a promising infrastructure, edge storage systems have drawn many attempts to efficiently distribute and share data among edge servers. However, it remains open to meeting the increasing demand for similarity retrieval across servers. The intrinsic reason is that the existing solutions can only return an exact data match for a query while more general edge applications require the data similar to a query input from any server. To fill this gap, this paper pioneers a new paradigm to support high-dimensional similarity search at network edges. Specifically, we propose Prophet, the first known architecture for similarity data indexing. We first divide the feature space of data into plenty of subareas, then project both subareas and edge servers into a virtual plane where the distances between any two points can reflect not only data similarity but also network latency. When any edge server submits a request for data insert, delete, or query, it computes the data feature and the virtual coordinates; then iteratively forwards the request through greedy routing based on the forwarding tables and the virtual coordinates. By Prophet, similar high-dimensional features would be stored by a common server or several nearby servers. Compared with distributed hash tables in P2P networks, Prophet requires logarithmic servers to access for a data request and reduces the network latency from the logarithmic to the constant level of the server number. Experimental results indicate that Prophet achieves comparable retrieval accuracy and shortens the query latency by 55%~70% compared with centralized schemes.
PENGARUH KEMAMPUAN KERJA DAN MOTIVASI KERJA TERHADAP KINERJA KARYAWAN (Studi pada Karyawan Operator PT. Indonesia Power Unit Bisnis Pembangkitan Semarang)

PT. Indonesia Power UBP Semarang, part of the PT. Indonesia Power, is engaged in power generating of gas and steam. Companies desperately need the ability and motivation of the workforce, especially in the technical and operational machinery. As a measure of the progress of the company, PT. Indonesia Power perform employee performance assessment. It conducted each semester by filling Form Employee Performance by superiors. From the preliminary survey conducted by the researchers, it is known that the performance of the employees of PT. Indonesia Power UBP Semarang in 2011 has decreased. Performance of employees was reduced in grade 4 from 14.74% to 7.98%. Absenteeism PT. Indonesia Power UBP Semarang in 2011 also declined. Employees who off work increased in June. This study aimed to determine the effect of work ability and work motivation on employee performance. The population in this study are 104 operator employees of PT. Indonesia Power UBP Semarang. Sampling technique in this study was saturated sampling. Measurement scale using Likert scale. Data analysis using regression with SPSS 17.0. The results showed that the ability variables have a positive and significant influence on the employees performance at 41.9 percent. Motivation variables have a positive and significant influence on the employees performance at 50.2 percent. Variable work ability and motivation have a positive and significant influence on the performance of employees at 53.5 percent. This study concluded that the ability to work and motivation have a positive and significant influence to the employee performance. PT. Indonesia Power UBP Semarang should provide training to improve the ability, especially in terms of mastery of work equipment and computer systems, understanding of company policies, understanding of corporate objectives and the ability of empathize. Provide better motivation to employees to create a feeling in the employees that they have a role in the company.
3D Printing of Personalised Carvedilol Tablets Using Selective Laser Sintering

Selective laser sintering (SLS) has drawn attention for the fabrication of three-dimensional oral dosage forms due to the plurality of drug formulations that can be processed. The aim of this work was to employ SLS with a CO2 laser for the manufacturing of carvedilol personalised dosage forms of various strengths. Carvedilol (CVD) and vinylpyrrolidone-vinyl acetate copolymer (Kollidon VA64) blends of various ratios were sintered to produce CVD tablets of 3.125, 6.25, and 12.5 mg. The tuning of the SLS processing laser intensity parameter improved printability and impacted the tablet hardness, friability, CVD dissolution rate, and the total amount of drug released. Physicochemical characterization showed the presence of CVD in the amorphous state. X-ray micro-CT analysis demonstrated that the applied CO2 intensity affected the total tablet porosity, which was reduced with increased laser intensity. The study demonstrated that SLS is a suitable technology for the development of personalised medicines that meet the required specifications and patient needs.
Best-Fit Circle for the Replication of Humeral Head Anatomy in Total Shoulder Arthroplasty

Background: Anatomic restoration of the humeral head is critical for successful shoulder replacement. Accurate measurements of the humeral head are essential for anatomic substitution. Purpose: To evaluate whether a best-fit circle of the humeral head, as determined from different projections of plain radiographs, can predict the humeral head implant size for either the left or right shoulder. Study Design: Descriptive laboratory study. Methods: Bilateral shoulder radiographs of 200 patients without arthropathy or other abnormal findings of the humeral head were evaluated. The best-fit circle was obtained based on 3 points: the medial and lateral endpoints of the anatomic neck and the lateral cortex below the greater tuberosity. This circle was drawn on 5 different radiographic projections (shoulder anteroposterior [AP], glenoid AP, outlet, axillary, and 30° caudal tilt) of the left and right shoulders of each patient, and the radius of each circle was measured. Agreement in the best-fit circle radius between the left and right shoulders was statistically analyzed using the intraclass correlation coefficient (ICC). There were 2 independent blinded observers who performed each measurement twice to evaluate inter- and intraobserver reliability. Results: Overall agreement in the radius between the right and left shoulders was excellent (all ICCs ≥0.990). The ICCs according to the radiographic view were 0.990 (95% CI, 0.986-0.993) for shoulder AP, 0.992 (95% CI, 0.989-0.995) for glenoid AP, 0.996 (95% CI, 0.994-0.997) for outlet, 0.994 (95% CI, 0.991-0.996) for axillary, and 0.993 (95% CI, 0.990-0.995) for 30° caudal tilt. Interobserver ICCs demonstrated a high level of precision: 0.987 (95% CI, 0.978-0.993) for shoulder AP, 0.986 (95% CI, 0.974-0.992) for glenoid AP, 0.974 (95% CI, 0.954-0.985) for outlet, 0.991 (95% CI, 0.984-0.995) for axillary, and 0.987 (95% CI, 0.977-0.993) for 30° caudal tilt. Intraobserver ICCs demonstrated excellent test-retest reliability: 0.983 (95% CI, 0.970-0.991) for shoulder AP, 0.989 (95% CI, 0.980-0.994) for glenoid AP, 0.987 (95% CI, 0.978-0.993) for outlet, 0.985 (95% CI, 0.973-0.991) for axillary, and 0.970 (95% CI, 0.947-0.983) for 30° caudal tilt. Conclusion: The best-fit circle to calculate the ideal size of a humeral head implant was able to be determined from different projections of plain radiographs. Clinical Relevance: Anatomic restoration of a deformed humeral head can be achieved using the best-fit circle of the contralateral humeral head as obtained from plain radiographs. This method can facilitate preoperative planning and postoperative evaluation in the anatomic restoration of the shoulder to avoid the problems of overstuffing.
Genetic Landscape of Masticatory Muscle Tendon–Aponeurosis Hyperplasia

Limited mouth opening is a characteristic of masticatory muscle tendon-aponeurosis hyperplasia (MMTAH). Although genetic involvement is suspected where familial onset is frequently observed, the genetic background of MMTAH is yet to be elucidated. In this study, we conducted whole genome sequencing of 10 patients with MMTAH and their family members when available. We also conducted RNA sequencing of normal temporal tendon (as disease region) and Achilles tendon (as control region) from commercially available pig samples. We identified 51 genes that had rare variants in patients with MMTAH and were highly expressed in the temporal tendons of pigs. Among the 51 genes, 37 genes have not been reported to be causative for human genetic diseases so far. As an implication of genetic involvement in the pathogenesis of MMTAH, 21 of these 37 genes were identified in two independent families. In particular, PCDH1 and BAIAP3 were identified in one affected individual in a family and consistently segregated in unrelated family, indicating they could be candidate causative genes of MMTAH. Our findings will help elucidate the genetic landscape of MMTAH and provide insights into future possibilities for tendon regeneration treatment.
Institutional Ownership and a Firm’s Greenhouse Gas Emissions

This study examines whether institutional investors promote corporate environmental performance. Specifically, we investigate how institutional investors affect a firm’s greenhouse gas (GHG) emissions. Using data on US firms’ GHG emissions over the 2002-2019 period, we find that institutional ownership is negatively related to the firm’s GHG emissions. This negative relationship persists after adjusting for endogeneity. Our results suggest that institutional investors tend to have incentives to monitor managers to reduce the firm’s GHG emissions. Overall, we contribute to the literature on corporate finance by identifying institutional investors’ role in enhancing the corporate environmental performance.
Effectiveness of iliac vein stenting combined with endovenous laser treatment of recurrent varicose veins associated with iliac vein compression

Background Iliac vein compression syndrome (IVCS) is an underlying cause of varicose vein (VV) recurrence after venous surgery. However, the management of recurrent varicose veins (RVVs) combined with IVCS has rarely been reported. This study aimed to investigate the outcomes of a one-stop procedure to correct outflow obstruction and superficial reflux for patients with RVVs and IVCS. Methods A retrospective analysis was conducted of 102 consecutive patients diagnosed with RVVs. Computed tomography venography (CTV) was performed to confirm IVCS. The cases were divided into 2 groups: the IRVVs group, including patients with RVVs and IVCS (n=48), and the RVVs group, including patients with RVVs only (n=54). The characteristics, vein reflux, and clinical, etiological, anatomical, and pathophysiological (CEAP) distribution were investigated. Then, the IRVVs group patients who underwent endovenous laser ablation (EVLA) (n=39) were divided into a further 2 groups: the EVLA + S group (n=19), who received EVLA and stenting of iliac vein, and the EVLA group (n=20), who received EVLA treatment alone. The great/small saphenous vein (GSV/SSV) trunk occlusion, VV recurrence, visual analogue scale (VAS), and venous clinical severity score (VCSS) were investigated. Results The prevalence rate of femoral vein reflux was 81.2% in IRVVs group and 50% in RVVs group (P<0.05). In the IRVVs group, 72.9% of patients manifested as CEAP clinical class >3, which was higher than that in RVVs group (48.1%) (P<0.05). The 12-month GSV/SSV occlusion rate in the EVLA + S and EVLA groups were 94.7% and 90.0%, respectively. Totals of 9 patients in EVLA + S group and 6 patients in EVLA group had active venous ulcers, and the ulcer healing time in EVLA + S group was significantly shorter (27.22±7.12 vs. 46.67±9.83 days, P<0.05). The reductions in the VAS and VCSS values between baseline, 1 month, and 12 months in the EVLA + S group were more obvious than those in EVLA group (P<0.05). Conclusions The one-stop combination treatment of iliac venous stenting and EVLA in patients with RVVs and IVCS is safe and effective and provides prominent symptom relief, improved quality of life, and a more satisfactory ulcer healing than EVLA alone.
Rare Genetic Variant in SORL1 May Increase Penetrance of Alzheimer’s Disease in a Family with Several Generations of APOE - (cid:2) 4 Homozygosity

21 Background: ThemajorgeneticriskfactorforlateonsetAlzheimer’sdisease(AD)isthe APOE - (cid:2) APOE - homozygosity genetic To identify genetic factors that, next to - (cid:2) 4 homozygosity, to the development of AD. Methods: We identiﬁed a family with nine AD patients spanning four generations, with an inheritance pattern suggestive of autosomal dominant AD, with no variants in PSEN1 , PSEN2 , or APP . We collected DNA from four affected and seven unaffected family members and performed exome sequencing on DNA from three affected and one unaffected family members. Results: All affected family members were homozygous for the APOE - (cid:2) 4 allele. Statistical analysis revealed that AD onset in this family was signiﬁcantly earlier than could be expected based on APOE genotype and gender. Next to APOE - (cid:2) 4 homozygosity, we found that all four affected family members carried a rare variant in the VPS10 domain of the SORL1 gene, associated with A (cid:3) PP processing and AD risk. Furthermore, three of four affected family members carried a rare variant in the TSHZ3 gene, also associated with A (cid:3) PP processing. Affected family members presented between 61 and 74 years, with variable presence of microbleeds/cerebral amyloid angiopathy and electroencephalographic abnormalities. Conclusion: We hypothesize that next to APOE - (cid:2) 4 homozygosity, impaired SORL1 protein function, and possibly impaired TSHZ3 function, further disturbed A (cid:3) processing. The convergence of these genetic factors over several generations might clarify the increased AD penetrance and the autosomal dominant-like inheritance pattern of AD as observed in this family.
Analysis of a field oriented control based variable direct current link drive for electric vehicles

The performance and efficiency of electric vehicles (EVs) depend mostly on the EVs powertrains. Therefore, one of the most vital research areas is the efficiency analysis of the drivetrain parts in electric vehicles. In this work, two basic drivetrain configurations of EVs are considered and evaluated. Firstly, the one with constant direct current (DC) link voltage and second one having a bidirectional DC-DC converter which supplies variable DC link voltage to the inverter. In order to control a permanent magnet synchronous machine (PMSM), field oriented control (FOC) technique is performed. Furthermore, a bidirectional DC-DC converter with optimized parameters is designed and implemented with variable DC link voltage controller. Finally, a comparison is made taking into account both drivetrain configurations with details efficiency analysis using real time switches at wide operating points of the machine.