Miracle-dz/UniFAR-FTUs huggingface dataset:
Bacteriophage T4 Infection of Stationary Phase E. coli: Life after Log from a Phage Perspective

Virtually all studies of phage infections investigate bacteria growing exponentially in rich media. In nature, however, phages largely encounter non-growing cells. Bacteria entering stationary phase often activate well-studied stress defense mechanisms that drastically alter the cell, facilitating its long-term survival. An understanding of phage-host interactions in such conditions is of major importance from both an ecological and therapeutic standpoint. Here, we show that bacteriophage T4 can efficiently bind to, infect and kill E. coli in stationary phase, both in the presence and absence of a functional stationary-phase sigma factor, and explore the response of T4-infected stationary phase cells to the addition of fresh nutrients 5 or 24 h after that infection. An unexpected new mode of response has been identified. “Hibernation” mode is a persistent but reversible dormant state in which the infected cells make at least some phage enzymes, but halt phage development until appropriate nutrients become available before producing phage particles. Our evidence indicates that the block in hibernation mode occurs after the middle-mode stage of phage development; host DNA breakdown and the incorporation of the released nucleotides into phage DNA indicate that the enzymes of the nucleotide synthesizing complex, under middle-mode control, have been made and assembled into a functional state. Once fresh glucose and amino acids become available, the standard lytic infection process rapidly resumes and concentrations of up to 1011 progeny phage (an average of about 40 phage per initially present cell) are produced. All evidence is consistent with the hibernation-mode control point lying between middle mode and late mode T4 gene expression. We have also observed a “scavenger” response, where the infecting phage takes advantage of whatever few nutrients are available to produce small quantities of progeny within 2 to 5 h after infection. The scavenger response seems able to produce no more than an average of one phage per originally available cell, and few if any further progeny are produced by cells in this mode even if fresh nutrients are made available later.
What governs the North Atlantic salinity maximum in a global GCM?

Taking advantage of the rapid advance in ocean modeling, this study investigates the sea surface salinity maximum in the North Atlantic, using results from a model of the Consortium for Estimating the Circulation and Climate of the Ocean (ECCO). Salinity budget terms were computed at the model's integration time step and archived as monthly averages. The simulated mixed layer salinity budget provides the first quantitative evidence for the ocean's role in governing the sea surface salinity maximum in the North Atlantic. Our analysis reveals that ocean dynamics explains about half of the sea surface salinity variance, being of equal importance as surface forcing. The sea surface salinity maximum varies both seasonally and interannually, as a consequence of interplay among surface flux, advection, and vertical entrainment. Contribution from eddies and small‐scale processes is relatively weak but not negligible. These results may provide useful hints for the design and interpretation of future observations in the region.
Self-reported postwar injuries among Gulf War veterans.

OBJECTIVE From September 1995 to May 1996, the authors conducted a telephone survey of Iowa military personnel who had served in the regular military or activated National Guard or Reserve during the Gulf War period. To assess the association between military service in a combat zone and subsequent traumatic injury requiring medical consultation, the authors analyzed veterans' interview responses. METHODS Using data from the larger survey, the authors compared rates of self-reported postwar injuries requiring medical consultation in a sample of Iowa Gulf War veterans to the rates in a sample of Iowa military personnel who served at the same time, but not in the Persian Gulf. RESULTS Of 3695 veterans, 605 (16%) reported a traumatic injury in the previous three months requiring medical consultation. Self-reported injuries were associated with service in the Persian Gulf (odds ratio 1.26; 95% confidence interval 1.02, 1.55). CONCLUSION This finding is consistent with the results of earlier studies of traumatic injury mortality rates among war veterans.
Switched Controllability via Bumpless Transfer Input and Constrained Switching

In this note, we investigate the controllability of linear hybrid systems via bumpless transfer input and constrained switching. By bumpless transfer input, we mean that the control input signals are as close as possible at switching times. By constrained switching, we mean that the switching index sequence is cyclic and the switching time sequence is possibly with pre-assigned duration intervals. While the problem is well motivated in several practical situations, it is also theoretically interesting. A complete criterion for controllability is presented, and a computational procedure is developed for finding a switching signal and a control input to achieve the controllability.
RISA: Distributed Road Information Sharing Architecture

With the advent of the new IEEE 802.11p DSRC/WAVE radios, Vehicle-to-Vehicle (V2V) communications is poised for a dramatic leap. A canonical application for these future vehicular networks is the detection and notification of anomalous road events (e.g., potholes, bumps, icy road patches, etc.). We present the Road Information Sharing Architecture (RISA), the first distributed approach to road condition detection and dissemination for vehicular networks. RISA provides for the in-network aggregation and dissemination of event information detected by multiple vehicles in a timely manner for improved information reliability and bandwidth efficiency. RISA uses a novel Time-Decay Sequential Hypothesis Testing (TD-SHT) approach in which event information from multiple sources is combined with time-varying beliefs. We describe our implementation of RISA which has been deployed and tested on a fleet of vehicles on-site at the GM Warren Technical Center in Michigan. We further provide a comprehensive evaluation of the aggregation mechanism using emulation of the RISA code on real vehicular mobility traces.
Inhibition of B‐Cell Function by Concanavalin‐A‐Induced Suppressor Cells

The in vitro immune response of normal spleen cells to the thymus‐independent antigen dinitrophenylated levan (DNP‐LE) was found to be inhibited by suppressor cells induced previously by concanavalin A (Con A) in vitro. At various suppressor to target cell ratios, the level of suppression observed was comparable to that seen in anti‐sheep erythrocyte (SRBC) responses. Consequently, it is suggested that B cells constitute a direct target for Con‐A‐induced suppressor cell activity.
Neuropsychiatric Inventory data in a Belgian sample of elderly persons with and without dementia

Background/aims This study assesses and compares prevalence of psychological and behavioral symptoms in a Belgian sample of people with and without dementia. Methods A total of 228 persons older than 65 years with dementia and a group of 64 non-demented persons were assessed using the Neuropsychiatric Inventory (NPI) in 2004. Results Within the group without dementia, the most frequent symptoms were depression, agitation, and irritability. Within the group with dementia, the most common symptoms were depression, irritability, apathy, and agitation. Prevalence of delusions (P < 0.05), hallucinations (P < 0.05), anxiety (P < 0.05), agitation (P < 0.05), apathy (P < 0.01), aberrant motor behavior (P < 0.01), and eating disorders (P < 0.05) were significantly higher in the group with dementia. Conclusion Depression, elation, irritability, disinhibition, and sleeping disorders are not specific to dementia. Agitation, apathy, anxiety, and delusions are more frequent in dementia but were not specific to the dementia group because their prevalence rates were close to 10% in the group without dementia. Hallucinations, aberrant motor behavior, and eating disorders are specific to dementia. The distinction between specific and nonspecific symptoms may be useful for etiological research on biological, psychological, and environmental factors.
Hiv Infection, Antiretroviral Therapy

In the last 15 years, highly active antiretroviral therapy (HAART) has determined a dramatic reduction of both morbidity and mortality in human immunodeficiency virus (HIV) subjects, transforming this infection in a chronic and manageable disease. with HIV in the developed world, in larger number men, are becoming aged. As it would be expected for a population of comparable age, many HIV history of cardiovascular disease, a small proportion h cardiovascular event and an increasing proportion has diabetes mellitus. Smoking rate is very high while an increasing proportion of HIV suggest that these traditional risk factors cardiovascular disease in these patients as they do in the general population. Thus, whilst the predicted 10-year cardiovascular disease risk remains relatively low at present, it will likely increase in relation to the progressive aging of this patient population. Thus, the long follow-up of HIV infected patients has to include co cardiovascular disease prevention and treatment. Two intriguing aspects related to the cardiovascular risk in patients with HIV infection are the matter of current investigation: 1) while these subjects share many cardiovascular risk factors with the general population, HIV infection itself increases cardiovascular risk; 2) some HAART regimens t atherosclerotic profile, partly due to lipid changes. Although the mechanisms involved in the development of cardiovascular complications in HIV elucidated, treatment guidelines recommending interventions t disease in these individuals are already available; however, their application is still limited. www.mjhid. org ISSN 2035-3006 and Cardiovascular Risk 1 and Licia Iacoviello Department of Infectious Diseases, Catholic University Medical -06-30155372, fax +39 at no competing interests exist. 34, DOI 10.4084/MJHID.2010. 034 ), which permits unrestricted use, distribution, and reproduction in any -infected individuals report a family ave already experienced a -infected individuals have dyslipidaemia. Studies could play an important role in the development of -morbidity management such as -infected patients remain to be fully o prevent cardiovascular -06-3054519, e-mail: -infected Patients surviving
Thymidylate synthase expression in advanced colorectal cancer predicts for response to raltitrexed.

PURPOSE The purpose of this research was to evaluate the predictive value of expression of thymidylate synthase (TS) and other genes for response to raltitrexed (RTX). EXPERIMENTAL DESIGN Twenty-five patients with metastatic colorectal cancer received RTX 3 mg/m(2) 3-weekly. Pretreatment tumor biopsies were analyzed for TS, dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), folylpolyglutamate synthetase, and reduced folate carrier mRNA expression by real-time reverse transcription-PCR. TS protein expression was evaluated by immunohistochemistry using a polyclonal TS antibody. RESULTS Twenty patients were evaluable for response and gene expression. Six of 20 (30%) achieved a partial response. Median TS/beta-actin was 5.7 x 10(3) (range, 2.2-42 x 10(3)). Median TS/beta-actin was 3.7 x 10(3) in responding patients and 6.1 x 10(3) in nonresponders (P = 0.048). Five of 6 patients with TS/beta-actin </=4.1 x 10(3) and 1 of 14 with higher values responded (P = 0.002). Overall survival was 21.7 months in patients with TS/beta-actin </=4.1 x 10(3) and 5.7 months in patients with higher values (P = 0.013). No correlations were seen between expression of TP, DPD, reduced folate carrier, or folylpolyglutamate synthetase mRNA and response or survival. Weak TS staining was seen in 10 of 21 tumors evaluable for immunohistochemistry, including 5 responders All 4 of the patients with both weak staining and TS/beta-actin </=4.1 x 10(3) responded. CONCLUSIONS High TS mRNA expression predicts nonresponse to RTX. By contrast with 5-fluorouracil, high levels of TP and DPD mRNA are not associated with RTX resistance. Limited genomic fingerprinting could optimize single-agent therapy, allowing combinations to be reserved for high TS-expressing patients or for treatment failures, with potential reductions in toxicity and cost.
Time Synchronization Protocol in Wireless Sensor Network based on Hash Code

ABSTRACT In current time peoples are switching from wired network to wireless network. This is great achievement for technology. Peoples are using wireless networks, but there is main problem which arises in wireless network is security. Many researches are involved in this field. Wireless sensor networks (WSN) have achieved a lot of consideration recently due to wide range of research applications such as target tracking, environment monitoring, and scientific exploration in dodgy environments. Each sensor node in WSN contains a local clock, required for time synchronization. Time synchronization is a significant module of sensor networks to grant a common clock time in sensor nodes. Some sensor nodes may be harmful, which can disturb the normal function of a sensor network. In this paper, main focus is to uncover malicious nodes and propose time synchronization protocol based on hash code. Keywords Sensor Networks, Security, Time Synchronization, Malicious nodes, .
Pharmacological properties of the rhesus macaque monkey P2X7 receptor

BACKGROUND AND PURPOSE The human P2X7 (hP2X7) receptor exhibits striking pharmacological differences from its rodent counterparts, particularly in terms of its antagonist profile. Here, we characterized the functional and pharmacological properties of the rhesus macaque monkey P2X7 (rmP2X7) receptor in comparison with the hP2X7 receptor.
Groundwater flooding in a river‐connected alluvial aquifer

Groundwater flooding can occur when the water table rises due to (a) recharge to (or decreased abstraction from) aquifers with low storativity or (b) propagation of the rising river stages into permeable, river‐connected alluvial aquifers. The latter type was significant in 2013 in Alberta. A survey of 189 homes along the Elbow River in Calgary examined the basement flooding water characteristics and the initial route of floodwater entry. In homes where the initial route of entry was known, 88% were initially flooded by groundwater, and 12% reported exclusively groundwater flooding. Basement floor elevation was correlated with the severity of flooding (R2 = .61). Of the 19 surveyed homes located outside of the 100‐year overland flood zone, 47% were flooded by groundwater, indicating that groundwater flooding reaches beyond overland water‐flooded areas. Hydrogeological modelling demonstrated that propagation of increased river stage into the aquifer could reasonably have caused the observed groundwater flooding. Groundwater flood resilience strategies could include (a) monitoring groundwater levels in flood‐prone areas to differentiate sewer backup from groundwater flooding via wastewater collection systems and to provide groundwater flooding warnings, (b) specifying minimum home basement elevation with respect to river stages for specified flood return intervals, and (c) appropriate home construction.
An algebraic perspective to single-transponder underwater navigation

This paper studies the position estimation of an underwater vehicle using a single acoustic transponder. The chosen estimation approach is based on nonlinear differential algebraic methods which allow to express very simply conditions for observability. These are then used in combination with an integrator-based time-derivative estimation technique to design an algebraic estimator, which, contrary to asymptotic observers, does not require sometimes tedious convergence verification. Simple simulation results are presented to illustrate the approach.
An Online Discriminative Approach to Background Subtraction

We present a simple, principled approach to detecting foreground objects in video sequences in real-time. Our method is based on an on-line discriminative learning technique that is able to cope with illumination changes due to discontinuous switching, or illumination drifts caused by slower processes such as varying time of the day. Starting from a discriminative learning principle, we derive a training algorithm that, for each pixel, computes a weighted linear combination of selected past observations with time-decay. We present experimental results that show the proposed approach outperforms existing methods on both synthetic sequencse and real video data.
Stem cell biology of the central nervous system

Neural stem cells (NSCs) are multipotential progenitor cells that have self‐renewal activities. A single NSC is capable of generating various kinds of cells within the central nervous system (CNS), including neurons, astrocytes, and oligodendrocytes. Because of these characteristics, there is increasing interest in NSCs and neural progenitor cells from the aspects of both basic developmental biology and therapeutic applications to the damaged brain. This special issue, dedicated to understanding the nature of the NSCs present in the CNS, presents an introduction to several avenues of research that may lead to feasible strategies for manipulating cells in situ to treat the damaged brain. The topics covered by these studies include the extracellular factors and signal transduction cascades involved in the differentiation and maintenance of NSCs, the population dynamics and locations of NSCs in embryonic and adult brains, prospective identification and isolation of NSCs, the induction of NSCs to adopt particular neuronal phenotypes, and their transplantation into the damaged CNS. © 2002 Wiley‐Liss, Inc.
Two Novel Defenses against Motion-Based Keystroke Inference Attacks

Nowadays smartphones come embedded with multiple motion sensors, such as an accelerometer, a gyroscope and an orientation sensor. With these sensors, apps can gather more information and therefore provide end users with more functionality. However, these sensors also introduce the potential risk of leaking a user's private information because apps can access these sensors without requiring security permissions. By monitoring a device's motion, a malicious app may be able to infer sensitive information about the owner of the device. For example, related work has shown that sensitive information entered by a user on a device's touchscreen, such as numerical PINs or passwords, can be inferred from accelerometer and gyroscope data. In this paper, we study these motion-based keystroke inference attacks to determine what information they need to succeed. Based on this study, we propose two novel approaches to defend against keystroke inference attacks: 1) Reducing sensor data accuracy; 2) Random keyboard layout generation. We present the design and the implementation of these two defences on the Android platform and show how they significantly reduce the accuracy of keystroke inference attacks. We also conduct multiple user studies to evaluate the usability and feasibility of these two defences. Finally, we determine the impact of the defences on apps that have legitimate reasons to access motion sensors and show that the impact is negligible.
Alignment Verification in the Early Stage of Service Design

Verification is a costly task, sometimes burdensome and tedious, requiring strong formal background. To reduce the effort and cost invested in verification, we developed a modeldriven approach for automatic verification of service properties, done in the early service design phase. Our approach is based on SEAM, a service modeling method, and it incorporates a verification system called Leon. With our approach service designers do not need substantial understanding of specific formal and verification languages, since the SEAM visual service model is both the input for and output of the alignment verification.
Evolving black box recursive modeling algorithm

In this paper an evolving recursive fuzzy cluster algorithm based on maximum likelihood criterion using the recursive instrumental variable parameter estimation for nonlinear system identification, is proposed. The performance of the proposed methodology is illustrated for black box modeling of a thermal plant from real-time acquisition data platform. The experimental results are evaluated from metrics used in the literature to show the efficiency of the proposed online evolving recursive fuzzy clustering algorithm.
Interaction of the von Willebrand Factor (vWF) with Collagen

The von Willebrand factor (vWF) mediates platelet adhesion to the vascular subendothelium by binding to collagen, other matrix constituents, and the platelet receptor glycoproteins Ib/IX and IIb/IIIa. Although substantial progress has been made in defining vWF structure-function relationships, there are conflicting data regarding the location of its collagen-binding site(s). Possible collagen-binding sites have been localized in the A1 and A3 domains of vWF. To study the proposed binding sites, we have expressed cDNA sequences encoding the A1 and A3 domains of vWF in Escherichia coli and purified the resulting proteins from bacterial inclusion bodies. In addition, a chimeric molecule containing residues 465-598 of the vWF A1 domain polypeptide (vWF-A1) fused in frame to residues 1018-1114 of the vWF A3 domain polypeptide (vWF-A3) was also expressed. Each of the three recombinant proteins purified as a monomer and contained a single disulfide bond. As previously reported (Cruz, M. A., Handin, R. I., and Wise, R. J. (1993) J. Biol. Chem. 268, 21238-21245), recombinant vWF-A1 inhibited ristocetin-induced platelet agglutination, but did not compete with vWF multimers for collagen binding. In contrast, vWF-A3 inhibited the binding of multimeric vWF to immobilized collagen, but did not inhibit ristocetin-induced platelet agglutination. Metabolically labeled vWF-A3 bound to immobilized collagen in a saturable and reversible manner with a Kof 1.8 10 M. The vWF-A1/A3 chimera was bifunctional. It inhibited vWF binding to platelet glycoprotein Ib/IX with an IC of 0.6 10 M and inhibited vWF binding to collagen with an IC of 0.5-1.0 10 M. These results, taken together, provide firm evidence that the major collagen-binding site in vWF resides in the A3 domain.
Correlation between a specific molecular weight form of plasminogen activator and metabolic activity of 3T3 cells

In quiescent cultures of 3T3 cells, plasminogen activator (PA) is found predominantly as a 75,000 dalton species. When quiescent cells are exposed to mitogenic agents such as phorbol myristate acetate, Ca++, or 25% serum, the absolute levels of PA in cell lysates may either increase or decrease. However, a consistent observation is that in the stimulated cultures PA is found predominantly as a 49,000 dalton species. This also is the predominant form of PA in growing and transformed cells. Concomitant with the mitogen-induced stimulation of the 49,000 dalton PA in quiescent cultures is a change in morphology to one that is characteristic of growing and transformed cells. The data suggest that PA is not operative in causing the morphological change that occurs with activation; however, the 49,000 dalton PA in particular is closely related to the pleiotypic response accompanying growth stimulation and transformation.