Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of cyclins in high‐density cultured cells and in vivo tumor cells

It is widely assumed that during the mammalian cell cycle, four major cyclins, including G1 cyclins (D, E) and mitotic cyclins (A, B1), are expressed in an orderly scheduled pattern in exponentially cultured cells. In high‐density cultured cells and in vivo growing cells, whether these cyclins are expressed in the same pattern remains unknown. In this study, we investigated the expression of cyclins by flow cytometry and western‐blotting in cultured MOLT‐4 and HepG2 cells at high‐density. We found that, in high‐density cultures, the expression levels of cyclin D, E, and A within each cell cycle phase was less than their levels in low‐density cultures. Cyclin B1 was slightly reduced in G2/M phase and unexpectedly expressed in G1 phase. The cyclin B1 expressed in G1 phase was localized mainly in the cytoplasm, and its level was positively correlated with apoptosis. Moreover, we obtained similar cyclins expression pattern in inoculated HepG2 tumor cells from immune‐deprived mice and leukemic cells from leukemia patients. These findings describe an altered pattern of cyclin expression in high‐density cultured cells as well as in vivo tumor cells. We suggest that high‐density cultures might mimic the in vivo growth conditions and such experimental situation may be useful for cell cycle research.
Research to develop spiritual pedagogy, awareness and change

ABSTRACT A co-operative inquiry group consisting of 8 counsellors met for 11 months to explore their experience of spirituality in their training and in their work with clients [Swinton, V. (2010). The spiritual in counselling training (Unpublished Thesis). The University of Manchester; Swinton, V. (2015). The spiritual in counselling training. In G. Nolan & W. West (Eds.), Therapy, culture and spirituality: Developing therapeutic practice. Basingstoke: Palgrave Macmillan]. The aim to explore whether spirituality was absent from the process of training, specifically to discover (1) how counsellors perceived and described their experience of spirituality in their training and (2) with a view to developing spiritual pedagogy into the training process of practitioners. The research was based on the Human Inquiry paradigm, where participants could consider the implications of new ideas for their own lives and their practice [Reason, R. , & Bradbury, H. (2001). Handbook of action research: Participative inquiry and practice. London: Sage]. From these groups although four main themes were identified, what is being considered in this paper is how the inquiry group process proved to be a useful vehicle for transformative learning and a dynamic means for individuals to engage with the topic in counselling training.
Spatial Perspective Taking is Robust in Later Life

In developmental studies of spatial perspective taking, it is important to clearly distinguish imagining body movement from other related cognitive information processing, to capture the genuine features of this ability in aging. This study examined the characteristics of these abilities in the older adults by comparing differences among age groups. A video game task was devised to evaluate response times from various angles of rotation. Four hundred twenty-eight healthy individuals aged 6 to 79 years (eight age groups at 10-year intervals) participated. Average response times for each age group confirmed a curvilinear change that accelerated from childhood to early adulthood and decelerated in later life. However, older participants did not display inferior performance compared with the younger adults on the response times to rotate an imaginary self to a 180° position. These results confirm previous findings that spatial perspective taking, particularly imagining body movement, remains robust in normal aging.
Charging behaviour of users utilising battery electric vehicles and extended range electric vehicles within the scope of a field test

Electric vehicles (EVs) and their application raise new questions and demands. Accordingly, the charging process of electric vehicles is a novelty in everyday life when using such vehicles. This paper presents results of user charging behavior within a field test. In the field test, 500 people with above average yearly mileage took part in integrating the vehicles into their everyday lives. The differences in user behavior between purely electric (BEV) and extended range electric vehicles (EREV) are discussed. Additionally, the opportunities and limitations for vehicles with combined grid services appear from the results as well as the resulting grid load. The results indicate that the vehicles are parked close to a charging station the majority of the time. The results also show deviating user behavior for the BEVs and EREVs.
Continuation Electroconvulsive Therapy With Pharmacotherapy Versus Pharmacotherapy Alone for Prevention of Relapse of Depression: A Randomized Controlled Trial

Objective The primary aim of the study was to test the hypothesis that relapse prevention with continuation electroconvulsive therapy (ECT) plus pharmacotherapy is more effective than pharmacotherapy alone after a course of ECT for depression. Methods A multicenter, nonblinded, randomized controlled trial with 2 parallel groups was performed from 2008 to 2012 in 4 hospitals in Sweden. Patients eligible had unipolar or bipolar depression and had responded to a course of ECT. The patients (n = 56) were randomly assigned (1:1) to receiving either 29 treatments of continuation ECT with pharmacotherapy or pharmacotherapy alone for 1 year. The pharmacotherapy consisted of antidepressants (98%), lithium (56%), and antipsychotics (30%). The main outcome was relapse of depression within 1 year. Relapse was defined as 20 or more points on the Montgomery Åsberg Depression Rating Scale or inpatient psychiatric care or suicide or suspected suicide. All 56 patients randomized were analyzed according to an intention to treat analysis. Results Sixty-one percent of the patients treated with pharmacotherapy versus 32% of the patients treated with ECT plus pharmacotherapy relapsed within 1 year (P = 0.036). The Cox proportional hazard ratio was 2.32 (1.03–5.22). Cognitive function and memory measures were stable for patients without relapse in both groups. One suspected suicide and 3 suicide attempts by intoxication occurred, all in the pharmacotherapy-alone group. Conclusions The post-ECT relapse rates were substantial in both treatment groups with a statistically significant advantage for combined treatment with pharmacotherapy and continuation ECT. Further studies are needed to define indications for continuation ECT, pharmacotherapy, and their combination.
Data security in medical information system

The database systems use the mechanisms of granting and revoking privileges and of authorization control to ensure the security of data. However, some users such as administrators or persons in charge of security have access to comprehensive content of database including patient's information that are of intimate nature and must be discreet. To enhance the security and avoid illegal access of such users, we propose in this article a mechanism using the content-based watermarking technique. Patient's information are encrypted and inserted in an image associated to it. This image, with faculties of object-relational and object-oriented databases, is directly integrated into the database. To check the integrity of the image we use the edge map and invariant moments.
PROTEUS and PUNDIT: RESEARCH IN TEXT UNDERSTANDING at the Department of Computer Science, New York University and System Development Corporation -- A Burroughs Company

We are engaged in the development of systems capable of analyzing short narrative messages dealing with a limited domain and extracting the information contained in the narrative. These systems are initially being applied to messages describing equipment failure. This work is a joint effort of New York University and the System Development Corp. for the Strategic Computing Program. Our aim is to create a system reliable enough for use in an operational environment. This is a formidable task, both because the texts are unedited (and so contain various errors) and because the complexity of any real domain precludes us from assembling a "complete" collection of the relationships and domain knowledge relevant to understanding texts in the domain.
Practice Degree of the Managerial Transparency in the Jordanian Private Universities as Viewed by Their Heads of Departments

This study aimed to identify extent of the administrative transparency implementation in the Jordanian private universities as viewed by the directors of the academic departments in these universities. To achieve the study aims, a 47-item questionnaire was developed, generally intended to identify the administrative transparency in the private universities. The validity and reliability of the instrument had been duly verified. The study sample consisted of (93) academic departments' directors who were chosen through the random stratified method from the academic departments in the Jordanian private universities during the 2010/2011 academic year. To answer the study questions, the means, standard deviations (SD's), and (ANOVA) analysis of the study sample individuals. The findings of the study indicated that the implementation of the administrative transparency in the Jordanian private universities is carried out to a wide extent. The study findings further showed that there are no statistically significant differences at (α<0.05) significance level in the means of the administrative transparency practicing in the Jordanian private universities that may be ascribed to the (academic rank, years of experience, type of faculty) variables. The study recommended deepening and increasing the awareness of practicing the administrative transparency with the academic cadres at the Jordan higher education institutions. Such awareness could be performed by training these cadres on the transparency practicing concept, and informing them about the experiments of the international universities and the successes they gained as a result of adopting and practicing of such transparency, through developing the values and trends to build an established organizational university culture, in order to stress on the transparency practice with all the university workers. This is essential for the purpose of getting rid of some misused social vals such as favoritism and patronage, which effectively contribute in constraining the transparency practicing, especially when it comes to appoint the administrative and academic cadres, decision making mechanism, and implementation thereof in these universities.
Patient-Centered Tablet Application for Improving Medication Adherence after a Drug-Eluting Stent

Background/aims This study’s objective was to evaluate a patient-centered educational electronic tablet application, “My Interventional Drug-Eluting Stent Educational App” (MyIDEA) to see if there was an increase in patient knowledge about dual antiplatelet therapy (DAPT) and medication possession ratio (MPR) compared to treatment as usual. Methods In a pilot project, 24 elderly (≥50 years old) research participants were recruited after a drug-eluting stent. Eleven were randomized to the control arm and 13 to the interventional arm. All the participants completed psychological and knowledge questionnaires. Adherence was assessed through MPR, which was calculated at 3 months for all participants who were scheduled for second and third follow-up visits. Results Relative to control, the interventional group had a 10% average increase in MPR. As compared to the interventional group, more patients in the control group had poor adherence (<80% MPR). The psychological data revealed a single imbalance in anxiety between the control and interventional groups. On average, interventional participants spent 21 min using MyIDEA. Discussion Consumer health informatics has enabled us to engage patients with their health data using novel methods. Consumer health technology needs to focus more on patient knowledge and engagement to improve long-term health. MyIDEA takes a unique approach in targeting DAPT from the onset. Conclusion MyIDEA leverages patient-centered information with clinical care and the electronic health record highlighting the patients’ role as a team member in their own health care. The patients think critically about adverse events and how to solve issues before leaving the hospital.
Psychosocial work environment and stress-related disorders, a systematic review.

BACKGROUND Knowledge on the impact of the psychosocial work environment on the occurrence of stress-related disorders (SRDs) can assist occupational physicians in the assessment of the work-relatedness of these disorders. AIMS To systematically review the contribution of work-related psychosocial risk factors to SRDs. METHODS A systematic review of the literature was carried out by searching Medline, PsycINFO and Embase for studies published up until October 2008. Studies eligible for inclusion were prospective cohort studies or patient-control studies of workers at risk for SRDs. Studies were included in the review when data on the association between exposure to psychosocial work factors and the occurrence of SRDs were presented. Where possible, meta-analysis was conducted to obtain summary odds ratios of the association. The strength of the evidence was assessed using four levels of evidence. RESULTS From the 2426 studies identified, seven prospective studies were included in this review. Strong evidence was found that high job demands, low job control, low co-worker support, low supervisor support, low procedural justice, low relational justice and a high effort-reward imbalance predicted the incidence of SRDs. CONCLUSIONS This systematic review points to the potential of preventing SRDs by improving the psychosocial work environment. However, more prospective studies are needed on the remaining factors, exposure assessment and the relative contributions of single factors, in order to enable consistent assessment of the work-relatedness of SRDs by occupational physicians.
An exponentially tapered transmission line antenna

The analysis and design of an exponentially tapered transmission line antenna is presented. The exponentially tapered transmission line is designed to operate such that it has radiator characteristics at high frequency and serves as a matching section at low frequency. The NEC-2 is used to model the antenna at frequencies ranging from 500 MHz to 1 GHz to obtain the input impedance and the desired radiation pattern. >
Electrode placement configurations for 3D EIT

This paper investigates several configurations for placing electrodes on a 3D cylindrical medium to reconstruct 3D images using 16 electrode EIT equipment intended for use with a 2D adjacent drive protocol. Seven different electrode placement configurations are compared in terms of the following figures of merit: resolution, radial and vertical position error, image magnitude, immunity to noise, immunity to electrode placement errors, and qualitative evaluation of image artefacts. Results show that for ideal conditions, none of the configurations considered performed significantly better than the others. However, when noise and electrode placement errors were considered the planar electrode placement configuration (two rings of vertically aligned electrodes with electrodes placed sequentially in each ring) had the overall best performance. Based on these results, we recommend planar electrode placement configuration for 3D EIT lung imaging of the thorax.
Merit-infonomics Research Memorandum Series an Empirically-based Taxonomy of Dutch Manufacturing: Innovation Policy Implications an Empirically-based Taxonomy of Dutch Manufacturing: Innovation Policy Implications

The paper studies the degree of homogeneity of innovative behavior in order to determine empirically an industry classification of Dutch manufacturing that can be used for policy purposes. We use a two-limit tobit model with sample selection, which explains the decisions by business enterprises to innovate and the impact these decisions have on the share of innovative sales. The model is estimated for eleven industries based on the Dutch Standard Industrial Classification (SBI 1993). A likelihood ratio test (LR) is then performed to test for equality of the parameters across industries. We find that Dutch manufacturing consists of three groups of industries in terms of innovative behavior, a high-tech group, a low-tech group and the industry of wood, where firms seem to have a rather different innovative behavior from the remaining industries. The same pattern shows up in the three Dutch Community Innovation Surveys. Acknowledgements: The empirical part of this study has been carried out at the Centre for Research of Economic Microdata at Statistics Netherlands. The authors wish to thank Statistics Netherlands, and in particular Bert Diederen, for helping us in accessing and using the Mi-cronoom data set. The views expressed in this paper are solely those of the authors. The authors also wish to thank François Laisney, Patrick Waelbroek and participants at presentations in Maastricht, Strasbourg, Leuven and Lille for their helpful comments. The first author acknowledges financial support from METEOR.
Familial contexts of adolescent ego development.

In this paper we describe our newly constructed Constraining and Enabling Coding System (CECS). This scheme was constructed to identify family interactions that are conceptually relevant to adolescent ego development. First, we discuss the theoretical rationale and reliability properties of the codes. We then present results of applying the scales to observations of 61 families, consisting of 2 parents and an adolescent, drawn from matched high school and psychiatric populations. All families are upper and (predominantly) upper-middle class. Each member completed the Loevinger Sentence Completion Test and then participated in a revealed-differences task, using responses to Kohlberg Moral Dilemmas as discussion stimuli. Transcripts of these audiorecorded discussions formed the data base for the family analyses. After controlling for patient status and adolescent age, adolescent and parent ego-development scores still contributed to explained variance in family interactions. Adolescent ego development was positively associated with adolescent enabling behaviors (e.g., problem solving, empathy). In contrast, there were negative correlations between constraining behaviors (e.g., devaluing, withholding) and adolescent ego development. Parental behaviors were significantly associated with (a) parent ego development, and (b) adolescent ego development. The third dimension of our system, discourse change, also showed theoretically expected strong correlations between progressive discourse change and ego development. In our discussion we comment on the importance of now proceeding to sequence analyses in order to explore questions of directionality.
Fascin controls neuronal class-specific dendrite arbor morphology

The branched morphology of dendrites represents a functional hallmark of distinct neuronal types. Nonetheless, how diverse neuronal class-specific dendrite branches are generated is not understood. We investigated specific classes of sensory neurons of Drosophila larvae to address the fundamental mechanisms underlying the formation of distinct branch types. We addressed the function of fascin, a conserved actin-bundling protein involved in filopodium formation, in class III and class IV sensory neurons. We found that the terminal branchlets of different classes of neurons have distinctive dynamics and are formed on the basis of molecularly separable mechanisms; in particular, class III neurons require fascin for terminal branching whereas class IV neurons do not. In class III neurons, fascin controls the formation and dynamics of terminal branchlets. Previous studies have shown that transcription factor combinations define dendrite patterns; we find that fascin represents a downstream component of such programs, as it is a major effector of the transcription factor Cut in defining class III-specific dendrite morphology. Furthermore, fascin defines the morphological distinction between class III and class IV neurons. In fact, loss of fascin function leads to a partial conversion of class III neurons to class IV characteristics, while the reverse effect is obtained by fascin overexpression in class IV neurons. We propose that dedicated molecular mechanisms underlie the formation and dynamics of distinct dendrite branch types to elicit the accurate establishment of neuronal circuits.
Expression and processing of an unusual tRNA gene cluster in the cyanobacterium Anabaena sp. PCC 7120.

Anabaena sp. PCC 7120 is a filamentous cyanobacterium that bears a cluster of 26 tRNA genes and pseudogenes in the delta plasmid. The sequences of these tRNAs suggest that they have been acquired by horizontal gene transfer from another organism. The cluster is transcribed as a single transcript that is quickly processed to individual tRNAs. RNase P and RNase Z, in vitro, are able to process precursors containing some of these tRNAs. Deletion of the cluster causes no obvious phenotype or effect on growth under diverse culture conditions, indicating that the tRNAs encoded in the cluster are not required for growth under laboratory conditions, although they are aminoacylated in vivo. We have studied a possible tRNA(Ser) [tRNA(Ser) GCU(2)] present in the cluster with a sequence that deviates from consensus. This tRNA is processed in vitro by RNase P at the expected position. In addition, this tRNA(Ser) GCU is specifically aminoacylated with serine by an Anabaena sp. PCC 7120 crude extract. These data indicate that tRNA(Ser) GCU(2) is fully functional, despite its unusual structure. Similar clusters are found in other three cyanobacteria whose genomes have been sequenced.
Sémantique à large couverture et raisonnement spatio-temporelle [Wide-coverage semantics for spatio-temporal reasoning]

In this article, we describe our research on wide-coverage semantics for French- language texts and on its application to produce detailed semantic descriptions of itineraries. Using a categorial grammar semi-automatically extracted from the French Treebank and a manually constructed semantic lexicon, the resulting parser computes discourse representation structures representing the meaning of arbitrary text. The main goal of this paper is to apply and specialize this general framework of wide-coverage semantics to the spatial and temporal organization of the Itipy corpus — a set of 19th century texts discussing voyages through the Pyrenees mountains. The implemented system gives satisfying results and opens the door to the integration with specialized extensions, such as a separate module computing the discourse relations between the textual units. RESUME. Dans cet article, nous donnons une description de notre recherche sur la semantique a large couverture pour le francais et, plus precisement, de la facon dont ces expressions se- mantiques sont utilisees pour donner l'interpretation spatio-temporelle des itineraires. En uti- lisant une grammaire categorielle extraite semi-automatiquement du French Treebank et un lexique semantique construit manuellement, l'analyseur convertit les analyses syntaxiques en DRS (discourse representation structures). Le but principal de cet article est l'application et la specialisation de cette methodologie generale au corpus Itipy contenant des recits de voyage du 19e siecle. La chaine de traitement complete donne des resultats tout a fait satisfaisants et laisse l'opportunite d'y ajouter des extensions specialisees, comme un composant qui calculerait les relations discursives entre les parties du texte.