Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibitory Potential of α-Amylase, α-Glucosidase, and Pancreatic Lipase by a Formulation of Five Plant Extracts: TOTUM-63

Controlling post-prandial hyperglycemia and hyperlipidemia, particularly by regulating the activity of digestive enzymes, allows managing type 2 diabetes and obesity. The aim of this study was to assess the effects of TOTUM-63, a formulation of five plant extracts (Olea europaea L., Cynara scolymus L., Chrysanthellum indicum subsp. afroamericanum B.L.Turner, Vaccinium myrtillus L., and Piper nigrum L.), on enzymes involved in carbohydrate and lipid absorption. First, in vitro inhibition assays were performed by targeting three enzymes: α-glucosidase, α-amylase, and lipase. Then, kinetic studies and binding affinity determinations by fluorescence spectrum changes and microscale thermophoresis were performed. The in vitro assays showed that TOTUM-63 inhibited all three digestive enzymes, particularly α-glucosidase (IC50 of 13.1 µg/mL). Mechanistic studies on α-glucosidase inhibition by TOTUM-63 and molecular interaction experiments indicated a mixed (full) inhibition mechanism, and higher affinity for α-glucosidase than acarbose, the reference α-glucosidase inhibitor. Lastly, in vivo data using leptin receptor-deficient (db/db) mice, a model of obesity and type 2 diabetes, indicated that TOTUM-63 might prevent the increase in fasting glycemia and glycated hemoglobin (HbA1c) levels over time, compared with the untreated group. These results show that TOTUM-63 is a promising new approach for type 2 diabetes management via α-glucosidase inhibition.
High temperature inhibited the accumulation of anthocyanin by promoting ABA catabolism in sweet cherry fruits

Color is an essential appearance characteristic of sweet cherry (Prunus avium L.) fruits and mainly determined by anthocyanin. Temperature plays an important role in the regulation of anthocyanin accumulation. In this research, anthocyanin, sugar, plant hormone and related gene expression were analyzed using physiological and transcriptomic methods in order to reveal the effects of high temperature on fruit coloring and the related mechanism. The results showed that high temperature severely inhibited anthocyanin accumulation in fruit peel and slowed the coloring process. The total anthocyanin content in fruit peel increased by 455% and 84% after 4 days of normal temperature treatment (NT, 24°C day/14°C night) and high temperature treatment (HT, 34°C day/24°C night), respectively. Similarly, the contents of 8 anthocyanin monomers were significantly higher in NT than in HT. HT also affected the levels of sugars and plant hormones. The total soluble sugar content increased by 29.49% and 16.81% in NT and HT, respectively, after 4 days of treatment. The levels of ABA, IAA and GA20 also increased in both the two treatments but more slowly in HT. Conversely, the contents of cZ, cZR and JA decreased more rapidly in HT than in NT. The results of the correlation analysis showed that the ABA and GA20 contents were significantly correlated with the total anthocyanin contents. Further transcriptome analysis showed that HT inhibited the activation of structural genes in anthocyanin biosynthesis as well as the repression of CYP707A and AOG, which dominated the catabolism and inactivation of ABA. These results indicate that ABA may be a key regulator in the high-temperature-inhibited fruit coloring of sweet cherry. High temperature induces higher ABA catabolism and inactivation, leading to lower ABA levels and finally resulting in slow coloring.
Drug-induced mitochondrial toxicity: Risks of developing glucose handling impairments

Mitochondrial impairment has been associated with the development of insulin resistance, the hallmark of type 2 diabetes mellitus (T2DM). However, the relationship between mitochondrial impairment and insulin resistance is not fully elucidated due to insufficient evidence to support the hypothesis. Insulin resistance and insulin deficiency are both characterised by excessive production of reactive oxygen species and mitochondrial coupling. Compelling evidence states that improving the function of the mitochondria may provide a positive therapeutic tool for improving insulin sensitivity. There has been a rapid increase in reports of the toxic effects of drugs and pollutants on the mitochondria in recent decades, interestingly correlating with an increase in insulin resistance prevalence. A variety of drug classes have been reported to potentially induce toxicity in the mitochondria leading to skeletal muscle, liver, central nervous system, and kidney injury. With the increase in diabetes prevalence and mitochondrial toxicity, it is therefore imperative to understand how mitochondrial toxicological agents can potentially compromise insulin sensitivity. This review article aims to explore and summarise the correlation between potential mitochondrial dysfunction caused by selected pharmacological agents and its effect on insulin signalling and glucose handling. Additionally, this review highlights the necessity for further studies aimed to understand drug-induced mitochondrial toxicity and the development of insulin resistance.
Engineered Bone Marrow Stem Cell-Sheets Alleviate Renal Damage in a Rat Chronic Glomerulonephritis Model

Although mesenchymal stem cell (MSC)-based regenerative therapy is being developed for the treatment of kidney diseases, cell delivery and engraftment still need to be improved. Cell sheet technology has been developed as a new cell delivery method, to recover cells as a sheet form retaining intrinsic cell adhesion proteins, which promotes its transplantation efficiency to the target tissue. We thus hypothesized that MSC sheets would therapeutically reduce kidney disease with high transplantation efficiency. When the chronic glomerulonephritis was induced by two injections of the anti-Thy 1.1 antibody (OX-7) in rats, the therapeutic efficacy of rat bone marrow stem cell (rBMSC) sheet transplantation was evaluated. The rBMSC-sheets were prepared using the temperature-responsive cell-culture surfaces and transplanted as patches onto the surface of two kidneys of each rat at 24 h after the first injection of OX-7. At 4 weeks, retention of the transplanted MSC-sheets was confirmed, and the animals with MSC-sheets showed significant reductions in proteinuria, glomerular staining for extracellular matrix protein, and renal production of TGFß1, PAI-1, collagen I, and fibronectin. The treatment also ameliorated podocyte and renal tubular injury, as evidenced by a reversal in the reductions of WT-1, podocin, and nephrin and by renal overexpression of KIM-1 and NGAL. Furthermore, the treatment enhanced gene expression of regenerative factors, and IL-10, Bcl-2, and HO-1 mRNA levels, but reduced TSP-1 levels, NF-kB, and NAPDH oxidase production in the kidney. These results strongly support our hypothesis that MSC-sheets facilitated MSC transplantation and function, and effectively retarded progressive renal fibrosis via paracrine actions on anti-cellular inflammation, oxidative stress, and apoptosis and promoted regeneration.
Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid

Thermoplastic molded regenerated silk fibroin was proposed as a structural material in tissue engineering applications, mainly for application in bone. The protocol allows us to obtain a compact non-porous material with a compression modulus in the order of a Giga Pascal in dry conditions (and in the order of tens of MPa in wet conditions). This material is produced by compressing a lyophilized silk fibroin powder or sponge into a mold temperature higher than the glass transition temperature. The main purpose of the produced resin was the osteofixation and other structural applications in which the lack of porosity was not an issue. In this work, we introduced the use of citric acid in the thermoplastic molding protocol of silk fibroin to obtain porosity inside the structural material. The citric acid powder during the compression acted as a template for the pore formation. The mean pore diameter achieved by the addition of the higher amount of citric acid was around 5 μm. In addition, citric acid could effectively crosslink the silk fibroin chain, improving its mechanical strength. This effect was proved both by evaluating the compression modulus (the highest value recorded was 77 MPa in wet conditions) and by studying the spectra obtained by Fourier transform infrared spectroscopy. This protocol may be applied in the near future to the production of structural bone scaffolds.
Exploring the mechanism of consumer purchase intention in a traditional culture based on the theory of planned behavior

There has been a growing interest among scholars and practitioners in cultural empowerment due to the importance of this subject. In this study, we aim to explore the connection between traditional cultural symbols and cultural identity, further estimating how two variables stimulate consumers’ emotional value to generate consumers’ purchase intention. Based on existing traditional cultural literature and the theory of planned behavior (TPB), we first proposed a research framework and then empirically tested the relationship among traditional culture symbols, cultural identity, emotional value, and consumers’ purchase intention. The survey data was tested using structural equation modeling (SEM) and the following conclusions were drawn. First, the cognition of traditional cultural symbols and cultural identity has a direct and significant impact on the emotional value thereby, eliciting consumers’ purchase intention. Second, traditional cultural symbols are directly and indirectly (i.e., through emotional value or cultural identity) positively associated with consumers’ purchase intention, also cultural identity is directly and indirectly (i.e., through emotional value) associated with consumer purchase intention. Finally, emotional values mediate the indirect effect of traditional culture and cultural identity on purchase intention, and cultural identity plays a moderating role between traditional cultural symbols and consumers’ purchase intention. Our findings help to expand the existing literature on consumer purchase intentions by rationally using traditional cultural symbols in the product design and suggesting relevant marketing strategies. The research results can provide valuable inspiration for promoting the sustainable development of the national tidal market and repeating consumers’ purchasing intentions.
Neuronal Cell Types in the Spinal Trigeminal Nucleus of the Camel Brain

Neurons in the spinal trigeminal nucleus of a camel were morphologically studied by the Golgi impregnation method. The neurons were classified based on the size and shape of their cell bodies, the density of their dendritic trees, and the morphology and distribution of their appendages. At least 12 morphological types of neurons were found in the camel spinal trigeminal nucleus, including the following: stalked, islets, octopus-like, lobulated, boat-like, pyramidal, multipolar, round, oval, and elongated neurons. These neurons exhibited large numbers of various forms of appendages that arise not only from their dendrites but also from their cell bodies. Moreover, neurons with unique large dilatations especially at their dendritic branching points were also reported. The neurons reported in this study displayed an array of different sizes and shapes and featured various forms of appendages arising from cell bodies and dendrites. Such morphologically distinctive neuronal cell types might indicate an evolutionary adaptation to pain and temperature processing pathways at the level of the spinal trigeminal nucleus in camels, which traditionally live in a very harsh climatic environment and are frequently exposed to painful stimuli.
Stability analysis for control systems with reset integrators

In feedback control systems, it may be beneficial to reset integrators. For example, the so-called Clegg integrator is a linear integrator whose output is reset to zero whenever its input crosses zero. Its sinusoidal describing function is 1.69//spl omega/ /spl ang/-38.1/spl deg/ indicating possible advantage over its linear counterpart. In this paper, we develop some stability criteria for such systems based upon integral quadratic constraint models of the reset action.
A theoretical study of the use of internal standards for precise isotopic analysis by the surface ionization technique Part II: Error relationships

Using an idealized model it is shown that, to minimize errors in double-spiked isotopic analyses, spike mass numbers should alternate with normal. The comprehensive error analysis which follows assumes that the individual ion currents are the independent varietes for statistical purposes: errors in these are obtained by combining (i) a coefficient of variation eP due to fluctuations in ion emission with (ii) a resistor noise index eR and (iii) a shot noise index eS: the noise indices are the ratios [error due to noise]/[error (i) due to emission fluctuation] for the largest ion beam in the spectrum. Methods of computing error multipliers (operating on eP) from eR, eS and the isotopic abundances, are developed. For a particular element each error multiplier can be regarded as a function of two variables, the ratio of the two spikes selected and the spike/normal ratio, which can then be optimized by numerical methods. Optimum values of spike composition and spike/normal ratio are given for observations of isotopic fractionation of lead, molybdenum and iron using various pairs of spike mass numbers. If both noise indices are taken to be zero the minimum error multipliers are not very much increased by deviations from the ideal model. When eR is given values corresponding to experience with a typical electrometer, it is shown that satisfactory results would be obtained on Pb and Mo with quite low ion currents, but that for iron, because of its difficult isotopic composition, the maximum ion current should be as large as possible. Some possible deviations from first-order fractionation theory are considered. A general algebraic analysis for the determination of radiogenic isotopes is given, and different approaches to fractionation computations are discussed.
Pulse-energy dynamics of passively mode-locked solid-state lasers above the Q -switching threshold

We have investigated the dynamical behavior of various passively mode-locked solid-state lasers by measuring how a modulation of the pump power affects the output power. We show theoretically and experimentally how the damping of the relaxation oscillations is reduced and finally becomes zero when the pump power is reduced so that the threshold for Q-switched mode locking is approached. For the first time to our knowledge, this method provides important information on the stability of mode locking above the Q-switched mode-locking threshold. It is applicable to lasers that are mode locked with slow-saturable absorbers. The results helped to explain the cause of unexpectedly low Q-switching thresholds in two cases. Also we obtain some useful spectroscopic information.
Modified Projective Synchronization among Three Modified Chen Chaotic Systems with Unicoupled Response System

This paper presents three modified Chen chaotic systems. Some basic dynamical properties are studied. Based on Lyapunov stability theory, modified projective synchronization and modified projective dislocated synchronization are applied to achieve synchronization among three modified Chen chaotic systems with unicoupled response system. These results show that three modified Chen systems can still keep the basic and typical nonlinear phenomena. Numerical simulations are given to show the effectiveness of these methods.
Persistent memory despite rapid contraction of circulating T Cell responses to SARS-CoV-2 mRNA vaccination

Introduction While antibodies raised by SARS-CoV-2 mRNA vaccines have had compromised efficacy to prevent breakthrough infections due to both limited durability and spike sequence variation, the vaccines have remained highly protective against severe illness. This protection is mediated through cellular immunity, particularly CD8+ T cells, and lasts at least a few months. Although several studies have documented rapidly waning levels of vaccine-elicited antibodies, the kinetics of T cell responses have not been well defined. Methods Interferon (IFN)-γ enzyme-linked immunosorbent spot (ELISpot) assay and intracellular cytokine staining (ICS) were utilized to assess cellular immune responses (in isolated CD8+ T cells or whole peripheral blood mononuclear cells, PBMCs) to pooled peptides spanning spike. ELISA was performed to quantitate serum antibodies against the spike receptor binding domain (RBD). Results In two persons receiving primary vaccination, tightly serially evaluated frequencies of anti-spike CD8+ T cells using ELISpot assays revealed strikingly short-lived responses, peaking after about 10 days and becoming undetectable by about 20 days after each dose. This pattern was also observed in cross-sectional analyses of persons after the first and second doses during primary vaccination with mRNA vaccines. In contrast, cross-sectional analysis of COVID-19-recovered persons using the same assay showed persisting responses in most persons through 45 days after symptom onset. Cross-sectional analysis using IFN-γ ICS of PBMCs from persons 13 to 235 days after mRNA vaccination also demonstrated undetectable CD8+ T cells against spike soon after vaccination, and extended the observation to include CD4+ T cells. However, ICS analyses of the same PBMCs after culturing with the mRNA-1273 vaccine in vitro showed CD4+ and CD8+ T cell responses that were readily detectable in most persons out to 235 days after vaccination. Discussion Overall, we find that detection of spike-targeted responses from mRNA vaccines using typical IFN-γ assays is remarkably transient, which may be a function of the mRNA vaccine platform and an intrinsic property of the spike protein as an immune target. However, robust memory, as demonstrated by capacity for rapid expansion of T cells responding to spike, is maintained at least several months after vaccination. This is consistent with the clinical observation of vaccine protection from severe illness lasting months. The level of such memory responsiveness required for clinical protection remains to be defined.
On the stability and convergence of a Galerkin reduced order model (ROM) of compressible flow with solid wall and far‐field boundary treatment

A reduced order model (ROM) based on the proper orthogonal decomposition (POD)/Galerkin projection method is proposed as an alternative discretization of the linearized compressible Euler equations. It is shown that the numerical stability of the ROM is intimately tied to the choice of inner product used to define the Galerkin projection. For the linearized compressible Euler equations, a symmetry transformation motivates the construction of a weighted L2 inner product that guarantees certain stability bounds satisfied by the ROM. Sufficient conditions for well‐posedness and stability of the present Galerkin projection method applied to a general linear hyperbolic initial boundary value problem (IBVP) are stated and proven. Well‐posed and stable far‐field and solid wall boundary conditions are formulated for the linearized compressible Euler ROM using these more general results. A convergence analysis employing a stable penalty‐like formulation of the boundary conditions reveals that the ROM solution converges to the exact solution with refinement of both the numerical solution used to generate the ROM and of the POD basis. An a priori error estimate for the computed ROM solution is derived, and examined using a numerical test case. Published in 2010 by John Wiley & Sons, Ltd.
Dietary Effects of a Short-Term Administration of Microalgae Blend on Growth Performance, Tissue Fatty Acids, and Predominant Intestinal Microbiota in Sparus aurata

Given the potential of microalgae as new aquafeed ingredients, this study focuses on using a blend of microalgae, Tisochrysis lutea, Nannochloropsis gaditana, and Scenedesmus almeriensis, as a dietary ingredient for feeding Sparus aurata juveniles. The growth performance, carcass composition, tissue fatty acid profile, and intestinal microbiota were evaluated after a 30 day-feeding period. A microalgae-free diet was used as control, and three experimental diets were formulated containing 5%, 15%, and 25% of the microalgae blend (MB-5%, MB-15%, and MB-25%, respectively). After 7, 15, and 30 days of feeding experimental diets, biological samples were taken. Growth performance and nutrient utilization were not significantly modified at the end of the experiment. Microalgae inclusion tended to decrease body lipids and affected the fatty acid profile, especially MB-25 diet increased DHA levels. Diet MB-25 promoted appropriate microbial diversity, favoring the presence of probiotic bacteria, such as Lactobacillus, and significantly influencing the fatty acid composition and lipid metabolism in fish. In conclusion, using a short pulse of dietary administration of 25% microalgal blend in S. aurata modulates the intestinal microbiota and lipid composition while maintaining growth performance.
Blu-ray Disc Pickup Head for Dual Layer

For dual-layer Blu-ray disc (BD) recording systems, the compensation of thickness differences is required. In addition the influence from the other layer should be considered. As a spherical compensation method, we have adopted a liquid crystal panel to fabricate a compact pickup head. Furthermore we have inserted a polarization diffractive optical element to reduce the influence from the other layer. In this paper, we report the readout test results of a dual-layer disc using a compact optical head.
Lower bound on the channel routing problems

A detailed analysis of how grid points are used in routing nets in the Manhattan model is presented. A new lower bound on the channel width that asymptotically matches the known upper bound d/sub m/+O(f) and, therefore, is existentially tight is established. The bound is established by introducing the notion of 'holes' and developing arguments on the supply and the demand of such a commodity inside a chosen region. Realizing that the hole is the grid point where only one nontrivial net is allowed to jog, it is seen that the more nets use grid points separately (without sharing) the stronger the lower bound is. <<ETX>>
Cumulant Based Modal Parameter Extraction of Structures

This paper describes a nonlinear deterministic estimator based on cumulants for the extraction of modal parameters. The signal analysed is composed of multiple exponentially damped real sinusoids in unknown additive noise. Cumulants reduce significantly the effects of noise and are also an efficient way of compressing the sampled data. In modal analysis a sensor may be unable to detect some modes of vibration due to its location. Cumulants estimated from real data sampled at different locations and instances are added directly together. This average cumulant function will contain the eigenvalues for all excited modes of vibration. Finding the frequencies and corresponding damping factors is therefore reduced to solving a single average cumulant function. The performance of the proposed estimator is examined and compared with the Eigensystem Realization Algorithm via simulations.