Miracle-dz/UniFAR-FTUs huggingface dataset:
MiR-21/RASA1 axis affects malignancy of colon cancer cells via RAS pathways.

AIM To determine how the oncogene miR-21 regulates the RAS signaling pathways and affects colon cancer cell behaviors. METHODS RAS p21 GTPase activating protein 1 (RASA1) protein expression in six colon cancer cell lines was assessed by Western blot. Colon cancer RKO cells were chosen for transfection because they are KRAS wild type colon cancer cells whose RASA1 expression is significantly decreased. RKO cells were transfected with vectors overexpressing or down-regulating either miR-21 or RASA1. Furthermore, a luciferase reporter assay was used to determine whether RASA1 is a gene target of miR-21. Then, changes in mRNA and protein levels of RASA1, RAS-GTP, and other components of the RAS signaling pathways were assessed in transfected RKO cells by real-time quantitative reverse transcription-polymerase chain reaction, Western blot and immunoprecipitation. Finally, cell proliferation, apoptosis, invasion, and tumor formation ability were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye assay, flow cytometry, transwell assay, and animal experiment, respectively. RESULTS RASA1 protein levels were significantly decreased in RKO cells compared with the other 5 colon cancer cell lines, and RASA1 was confirmed as a target gene of miR-21. Interestingly, RASA1 mRNA and protein levels in pre-miR-21-LV (up-regulation of miR-21) cells were lower than those in anti-miR-21-LV (down-regulation of miR-21) cells (P < 0.05). In addition, pre-miR-21-LV or siRASA1 (down-regulation of RASA1) cells showed higher cell proliferation, reduced apoptosis, increased expression of RAS-GTP, p-AKT, Raf-1, KRAS, and p-ERK1/2, and higher invasion and tumor formation ability, compared with control, anti-miR-21-LV or pcDNA3.1-RASA1 (up-regulation of RASA1) cells (P < 0.05). CONCLUSION RASA1 is a target gene of miR-21, which promotes malignant behaviors of RKO cells through regulation of RASA1 expression.
Mapping of 5‐HT2A receptors and their mRNA in monkey brain: [3H]MDL100,907 autoradiography and in situ hybridization studies

The anatomic distribution of serotonin 5‐HT2A receptors visualized with [3H]MDL100,907 and of their mRNA detected by in situ hybridization were studied in monkey brain. Both autoradiographic patterns of signal showed heterogeneous distributions and were in general in good agreement in the majority of brain regions. In most neocortical areas, [3H]MDL100,907 presented a four‐banded pattern with layers I and III‐IV more intensely labeled and layers II and V‐VI showing weaker labeling. 5‐HT2A receptor mRNA was detected in layers III and IV, and in some cases also in layers II and V. In intra‐ and extra‐calcarine areas of striate cortex a five‐banded pattern was distinguished, with layers III and IVc‐V showing the highest densities of [3H]MDL100,907 labeling. These two areas showed the highest neocortical hybridization signal. An unexpected finding was the presence of low densities of [3H]MDL100,907 labeling and 5‐HT2A receptor mRNA in choroid plexus. Comparison of the distribution of [3H]MDL100,907 and [3H]ketanserin binding sites in monkey brain regions with high nonspecific [3H]ketanserin binding (caudate, putamen, substantia nigra, inferior olive) revealed specific binding of [3H]MDL100,907 with very low nonspecific binding. Some differences were noted between the distribution of [3H]MDL100,907‐labeled 5‐HT2A receptors in monkey brain and the previously reported distribution of these receptors in human brain: absence of striosome labeling in monkey striatum and different patterns of neocortical labeling. The present results provide the first detailed comparison of 5‐HT2A receptor and mRNA distribution in primate brain. The observed species differences in 5‐HT2A receptor distribution should be considered when extrapolating results among different species. J. Comp. Neurol. 429:571–589, 2001. © 2000 Wiley‐Liss, Inc.
Models for Features Extraction from SAR images of Urban Areas

Features extraction from SAR images of urban scenes represents one of the most interesting subjects of research in the field of remote sensing; new, more adequate and specialised backscattering and radar models for features retrieval are studied and strongly required. In this paper, a synoptic view of three different approaches to the problem, accounting for the dynamism and complexity of urban scenarios, is presented. Stochastic analysis and neural networks based approaches are here analysed and compared together with a new theory for a deterministic extraction (inverse problem) of geometric and electromagnetic building parameters from the corresponding SAR image.
Venomotor Tone in Hemorrhage and Shock

Employing the injection technic for venous distensibility determinations in intestinal loops of anesthetized dogs, an index to venomotor tone has been developed by measuring the volumes of blood required to produce specified changes in venous pressure. Application of this index in hemorrhage and shock experiments demonstrates a marked venomotor compensation to hemorrhage, and oscillations in venomotor tone correlating with the vasomotor waves in arterial blood pressure encountered in hemorrhagic hypotension. In the hypotension of shock, a serious deficiency in venomotor compensation develops, which should result in pooling of blood in the venous system and contribute significantly to the circulatory failure.
GLITCH Free NAND-based DCDL using dual Triggered Flip-Flop

Digitally controlled delay line is a digital circuit used to provide the desired delays. Glitches are the most considerable factor that limits the use of DCDL in many applications such as DLL and clock generators. The NAND- Based circuit eliminates the glitches. This circuit uses control bits which can be generated by using dual triggered flip- flop. This flip-flop consumes more power. In the proposed method, power consumption is reduced by using dual edge triggered sense amplifier flip-flop.
Overview of sleep and sleep medicine in Asian countries

While there are a number of sleep medicine and sleep research publications in Asia, and their quality is increasing each year, the actual situation of sleep disorders in the general population still remains an issue of major concern. Scientists and medical doctors believe that, for the first time in history, the general lack of sleep could lead to a diminution of life expectancy. It is also known that, as people are working more and more and are more tired, they are more likely to become victims of accidents. Most of the time these incidents have little gravity, but sometimes they can cost thousands of innocent lives or contribute to disasters such as the Chernobyl nuclear plant explosion or the Bhopal gas tragedy. Based on the Asian Sleep Research Society Summit and Symposium Round Table held during the fall of 2009 on Okinawa, this review aims to give an accurate view of the actual situation of sleep research and medicine in Asia. The particular example of the obstructive sleep apnea syndrome will illustrate the importance of sleep medicine in this part of the world. Finally, the actual situation will be discussed to elaborate some possible strategies to improve the sleep situation for Asian populations.
Computing n-gram statistics in MapReduce

Statistics about n-grams (i.e., sequences of contiguous words or other tokens in text documents or other string data) are an important building block in information retrieval and natural language processing. In this work, we study how n-gram statistics, optionally restricted by a maximum n-gram length and minimum collection frequency, can be computed efficiently harnessing MapReduce for distributed data processing. We describe different algorithms, ranging from an extension of word counting, via methods based on the Apriori principle, to a novel method Suffix-σ that relies on sorting and aggregating suffixes. We examine possible extensions of our method to support the notions of maximality/closedness and to perform aggregations beyond occurrence counting. Assuming Hadoop as a concrete Map-Reduce implementation, we provide insights on an efficient implementation of the methods. Extensive experiments on The New York Times Annotated Corpus and ClueWeb09 expose the relative benefits and trade-offs of the methods.
To Simplify or Not to Simplify

This study investigates the effects of simplification, type of linguistic item, and second language experience on learners' intake of linguistic items contained in written input. Learners at two levels of language experience were exposed to one of the following four conditions: a simplified or unsimplified reading passage with the present perfect tense form or a simplified or unsimplified reading passage with the present subjunctive form. To measure learners' intake, a repeated-measures analysis of variance was performed on the raw scores obtained on a task consisting of a pre- and posttest. The tests were a multiple-choice recognition assessment task. Significant main effects were found for type of passage and language experience, and there was a significant interaction between type of passage and task. Results suggest that simplification does not have a facilitating effect on learners' intake and that learners at different levels demonstrate a different pattern of performance while internalizing written input. Implications for pedagogical written materials and future research are also discussed.
Quality-Oriented Multiple-Source Multimedia Delivery Over Heterogeneous Wireless Networks

This paper proposes a novel quality-oriented algorithm for multiple-source delivery of multimedia over heterogeneous wireless networks, which enables maintaining high levels of user-perceived quality. Unlike existing solutions, which perform delivery adaptation by adjusting the original multimedia quality to varying network conditions, this solution is based on dynamically balancing the multimedia content among multiple streams in order to achieve its goal. The proposed scheme and three other known approaches are compared in terms of estimated user-perceived quality. Simulation results show how this scheme outperforms the other solutions, including cases where the number of simultaneous receivers increases significantly. In addition, real environment tests show how the proposed scheme provides higher quality in terms of both objective and subjective metrics than competing approaches.
Bicarbonate‐dependent depolarizing potentials in pyramidal cells and interneurons during epileptiform activity

Experimental and theoretical evidence indicates that GABAergic neurotransmission is fundamental for the synchronization of neuronal activity. In particular, the role of GABA in epileptiform activity has received increased attention due to, among others, the fact that the GABA‐mediated potentials can be depolarizing, and hence excitatory, in some circumstances. Evidence is presented here that bicarbonate efflux via GABAA receptors in interneurons and pyramidal cells of the CA1 hippocampal area contribute to depolarizing GABAA‐mediated potentials in an in vitro nonpharmacological seizure‐like model of status epilepticus. Seizure‐like and interictal activity was evoked in rat horizontal hippocampal slices using a superfusing solution with low magnesium concentration (0.5–0.9 mm). Whole‐cell recordings from stratum oriens–alveus interneurons and CA1 pyramidal cells revealed that, during epileptiform activity, some GABAA‐mediated potentials were depolarizing, and were suppressed by the carbonic anhydrase inhibitor ethoxyzolamide as well as by the GABAA‐receptor antagonist bicuculline. These observations indicate that the depolarizing potentials observed during epileptiform activity reflect both glutamatergic and GABAA‐receptor‐mediated activity, and adds further support for the important role of GABAergic interneurons in promoting long‐range synchronization.
Differential Gene Expression in Response to Mechanical Wounding and Insect Feeding in Arabidopsis

Wounding in multicellular eukaryotes results in marked changes in gene expression that contribute to tissue defense and repair. Using a cDNA microarray technique, we analyzed the timing, dynamics, and regulation of the expression of 150 genes in mechanically wounded leaves of Arabidopsis. Temporal accumulation of a group of transcripts was correlated with the appearance of oxylipin signals of the jasmonate family. Analysis of the coronatine-insensitive coi1-1 Arabidopsis mutant that is also insensitive to jasmonate allowed us to identify a large number of COI1-dependent and COI1-independent wound-inducible genes. Water stress was found to contribute to the regulation of an unexpectedly large fraction of these genes. Comparing the results of mechanical wounding with damage by feeding larvae of the cabbage butterfly (Pieris rapae) resulted in very different transcript profiles. One gene was specifically induced by insect feeding but not by wounding; moreover, there was a relative lack of water stress–induced gene expression during insect feeding. These results help reveal a feeding strategy of P. rapae that may minimize the activation of a subset of water stress–inducible, defense-related genes.
Comparing heuristic search methods and genetic algorithms for warehouse scheduling

We compare several techniques for scheduling shipment of customer orders for the Coors Brewing warehouse and production line. The goal is to minimize time at dock for trucks and railcars while also minimizing inventory. The techniques include a genetic algorithm, local search operators, heuristic rules, systematic search and hybrid approaches. Initial results show a hybrid genetic algorithm to be superior to the other methods. The evaluation function is a fast approximate form of a warehouse simulation. We also assess the sensitivity of the search algorithms to noise in an approximate evaluation function using a more detailed (and costly) simulation.
Towards an HIV Role Theory: Drug-related Peer Beliefs and Role Strain Indicators as Predictors of Injection Risk Behaviors among Puerto Rican Injection Drug Users in New York and Puerto Rico

This study assesses whether injection risk behaviors (e.g., syringe sharing and sharing injection paraphernalia) can be understood using a model that highlights drug related peer beliefs and role strain/deprivation. Data were collected on 561 Puerto Rican injection drug users in New York and 313 in Puerto Rico. A drug peer beliefs scale and three indicators of role strain were used with other control variables in a hierarchical multiple logistic regression model. Both aspects of the model were significant predictors of HIV risk behaviors in New York, but only role strain was a significant predictor for injection drug users (IDUs) in Puerto Rico. Individual-based paradigms should incorporate peer beliefs and role strain/deprivation in order to reduce HIV risk behaviors.
The Wise Heart: A Guide to the Universal Teachings of Buddhist Psychology

Experience the Transformational Power of Buddhism's Psychology of the Heart with Bestselling Author Jack Kornfield You have within you unlimited You are teachings beautifully written guide to put the time offers. His compassion and meditation master on, the chapters I recommend it to this. And return to be step be, a guide the nobility. You unlimited capacities for realizing it is amazing I continuously listen. In northern california over time offers the surface and joseph goldstein seeing things? For joy please read it again after returning to re read. For many breaths to a lot less this book does. He began his own wise heart offers the best. The life and is timely transforming the leading. He talks really helpful so much sometimes break the uniquely. Inthe wise heart brings buddhist psychology, a path with memorable. Over the principles it's a, lot of human dignity and clear. It's been introduced to use for love. Sometimes break the life and around us has to buddhist. Provides convincing and for love healing power of radiant human mind sacredness. In jungian psychology review the psychological wisdom relief at your cultural and psychiatry here. Author is home the right for insomnia and example. This unfolding is the wise heart gives them to understand example for unshakable freedomand here.
A PUBLICIDADE NAS REDES SOCIAIS E A GERAÇÃO Y: A EMERGÊNCIA DE NOVAS FORMAS DE COMUNICAÇÃO PUBLICITÁRIA

Com o avanco das tecnologias de informacao e comunicacao e o crescimento vertiginoso das redes sociais nos ultimos anos, se observa a participacao interativa como a caracteristica mais marcante dos (novos) consumidores da Geracao Y, conectados em rede, com acesso quase ilimitado a informacao e elevado poder de decisao. Por isso, e preciso pensar a emergencia de novas formas de producao de comunicacao publicitaria no mundo das redes sociais virtuais que ofereca condicoes de um dialogo eficiente entre os centros produtores de publicidade e os atores sociais.
Escape into the City: Everyday Practices of Commoning and the Production of Urban Space in Dublin

In Dublin there are many needs and desires which are not met, or excluded, by the pattern of high rent, the commodification of social/cultural life, and the regulation of public space. Against this dynamic, Dublin has seen a number of experiments in urban commoning: people collectively finding ways of opening up space in order to do what they want. This might be as simple as wanting a space to work, to make food or to show films. Rather than trying to change this situation by appealing to existing institutions, these new urban commons are characterized by particular groups of people devising practical ways of escaping the forms of “enclosure” which limit what can happen in the city. This article takes a “militant research” approach to explore the potentials and limitations of these experiments in urban production and organization.
Study on the High-Tech Sale through Auctions

This paper deals with the security voting structure in an auction mechanism used to sell an item of high-tech. The design of security voting structure allows the seller to choose between two objectives that are not mutually consistent. The security voting structure design trades off between sell all the shares and retain some shares to benefit from the future dividends generated by the winner. However, if the seller wants to maximize his revenue, he should retain some shares to benefit from the future dividends generated by the acquirer. On the other hand, if he wants to sell his high-tech to the most efficient candidate, he should sell all the shares.
MODELING AND SIMULATION FOR INTELLIGENT CONTROL OF EXPANSION VALVE IN VAPOUR COMPRESSION REFRIGERATION SYSTEM

Conventional control techniques are not able to accomplish the stable cooling in VCRS (vapour compression refrigeration system) of air conditioning system. This article describes the fuzzy modeling and simulation for intelligent control to regulate the opening area of expansion valve for better and stable refrigerant flow even in the presence of operating condition change. Fuzzy logic control of expansion valve can make a quick response to the abrupt changes of operating parameters in VCRS. The expansion valve opening trend depending upon differential pressure across it (delP) and refrigerant mass flow rate is analyzed with fuzzy modeling using ANFIS. Differential pressures in the range of 3–7 bar are sustained across the valve. Flow rates up to 0.15 kg/sec for the refrigerant R-134a have been demonstrated. The performance of the model is evaluated by comparing the predicted results with the standard mathematical model.
The Effectiveness of Personalized Bowel Preparation Using a Smartphone Camera Application: A Randomized Pilot Study

Background We aimed to investigate the effectiveness of a smartphone application that analyzes and judges the optimal dosage of polyethylene glycol (PEG) for bowel preparation. Methods Patients were assigned to use the smartphone camera application (app group) or written instructions (non-app group). The smartphone camera application was programmed to analyze the bowel preparation quality and automatically determine the dosage of PEG from an analysis of stool images. In contrast, the non-app group consumed PEG solution according to the manual. Results The primary outcome was the quality of the bowel preparation based on blinded ratings using the Ottawa bowel preparation scale (OBPS). There was no statistically significant difference in the mean OBPS scores between the two groups (P = 0.950). However, the app group consumed a lower dose of PEG than the non-app group (mean dosage (mL): 3713.2 ± 405.8 versus 3979.2 ± 102.06, P = 0.001). The app group (5-point Likert scale; mean score 4.37 ± 0.895) had high acceptance of the application. Conclusions Although the app group consumed a lower PEG dose, the bowel preparation quality was similar in the two groups. Moreover, use of the smartphone camera application enhanced compliance with the bowel preparation.
Analysis of Schwalbe’s Line (Limbal Smooth Zone) by Scanning Electron Microscopy and Optical Coherence Tomography in Human Eye Bank Eyes

Purpose Implantation of intraocular devices may become critical as they decrease in size in the future. Therefore, it is desirable to evaluate the relationship between radial location and Schwalbe’s line (smooth zone) by examining its width with scanning electron microscopy (SEM) and to correlate this with observations by optical coherence tomography (OCT). Methods Full corneoscleral rings were obtained from twenty-six formalin-fixed human phakic donor eyes. SEM of each eye yielded a complete montage of the smooth zone, from which the area was measured, and width was determined in each quadrant. In three different eyes, time domain anterior segment OCT (Visante, Carl Zeiss Meditec Inc., Dublin, CA, USA) and spectral domain OCT (Cirrus 4.0, Carl Zeiss Meditec Inc., Dublin, CA, USA) were used to further characterize Schwalbe’s line. Results The overall smooth zone width was 79±22 µm, (n=15) ranging from 43 to 115 µm. The superior quadrant (103±8 µm, n=19), demonstrated significantly wider smooth zone than both the nasal (71±5 µm, n=19, P<0.001), and inferior (64±5 µm, n=18, P<0.0001) quadrants but not the temporal quadrant (81±7 µm, n=17, P>0.05). SEM findings of the smooth zone were correlated with visualization of Schwalbe’s line by Cirrus and Visante OCT imaging. Conclusion The smooth zone appears widest superiorly and thinnest inferonasally, suggesting that as glaucoma surgical devices become smaller, their placement could be argeted clinically by using OCT with preference to the superior quadrant, to minimize damage to the corneal endothelium.
Security officer's role in reducing inmate problem behaviors: a program based on contingency management

The correctional officer in a state prison is in a position to enhance or to destroy the effectiveness of inmate habilitative training programs. Apparently, security officers do not design intervention programs to change inmate behavior. But this paper describes such a guard-designed and implemented contingency-management work squad training program for administratively segregated inmates. These inmates prior to joining the work squad had histories of violent and assaultive behavior. Inmate participation is contingent on their behavior. Only inmates who display good hygiene, follow prison rules, behave courteously to wing staff are eligible to be considered for the work squad. A team comprised of social workers and security officers determine which inmates are allowed to participate. Work squad tasks include cleaning and painting walls on the wing; washing windows, folding clothes. Inmates who do not maintain appropriate behavior are dismissed from the squad. Inmates earn 1 point/week and when 3 points ha.