Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Activated ADP-ribosylation Factors on Golgi Morphology Require neither Activation of Phospholipase D1 nor Recruitment of Coatomer*

Nine mutations in the switch I and switch II regions of human ADP-ribosylation factor 3 (ARF3) were isolated from loss-of-interaction screens, using two-hybrid assays with three different effectors. We then analyzed the ability of the recombinant proteins to (i) bind guanine nucleotides, (ii) activate phospholipase D1 (PLD1), (iii) recruit coatomer (COP-I) to Golgi-enriched membranes, and (iv) expand and vesiculate Golgi in intact cells. Correlations of activities in these assays were used as a means of testing specific hypotheses of ARF action, including the role of PLD1 activation in COP-I recruitment, the role of COP-I in Golgi vesiculation caused by expression of the dominant activating mutant [Q71L]ARF3, and the need for PLD1 activation in Golgi vesiculation. Because we were able to find at least one example of a protein that has lost each of these activities with retention of the others, we conclude that activation of PLD1, recruitment of COP-I to Golgi, and vesiculation of Golgi in cells are functionally separable processes. The ability of certain mutants of ARF3 to alter Golgi morphology without changes in PLD1 activity or COP-I binding is interpreted as evidence for at least one additional, currently unidentified, effector for ARF action at the Golgi.
On calculating communication ability in consensus situation awareness

To determine the C2 (command and control) relationship networks nodes' communication ability in consensus situation awareness quantitatively, the communication process aims at situation sharing in consensus situation awareness is analyzed. Against the background of C2 relationship networks, formal description on the nodes' communication ability in consensus situation awareness is made based on the model of OODA and three basic experiential hypotheses is given. Then the quantifiable model of nodes' communication ability in consensus situation awareness is built, and the analysis results of the model can be well explained by real world experience.
Diabetes and function in different cognitive systems in older individuals without dementia.

OBJECTIVE To examine the relation of type 2 diabetes to the level of function in five different cognitive systems in older individuals without dementia. RESEARCH DESIGN AND METHODS Participants were 882 older men and women without dementia participating in the Rush Memory and Aging Project, a longitudinal clinical-pathological study of aging and dementia. They underwent uniform evaluations, which included clinical classification of dementia, and detailed cognitive function testing from which previously established summary measures of episodic memory, semantic memory, working memory, perceptual speed, visuospatial ability, and global cognition were derived. Diabetes was identified by history and direct medication inspection. RESULTS Diabetes was present in 116 (13%) participants. In separate linear regression models controlling for age, sex, and education, diabetes was associated with lower levels of semantic memory (P < 0.001) and perceptual speed (P = 0.005), but not with episodic memory, working memory, or visuospatial ability or with a measure of global cognition. The associations of diabetes with cognition were reduced when controlling for several vascular variables, and the associations were substantially stronger in current smokers than in individuals who never smoked or formerly smoked. CONCLUSIONS These results suggest that type 2 diabetes is associated with cognitive impairment, especially in semantic memory and perceptual speed and that these effects may be modified by smoking status.
The Real-Time Status of Island Phenomena

In parasitic-gap constructions an illicit gap inside a syntactic island becomes acceptable in combination with an additional licit gap, a result that has interesting implications for theories of grammar. Such constructions hold even greater interest for the question of the relation between grammatical knowledge and real-time language processing. This article presents results from two experiments on parasitic-gap constructions in English in which the parasitic gap appears inside a subject island, before the licensing gap. An off-line studyconfirms that parasitic gaps are acceptable when theyoccur inside the infinitival complement of a subject NP, but not when they occur inside a finite relative clause. An on-line self-paced reading studyusing a plausibility manipulation technique shows that incremental positing of gaps inside islands occurs in just those environments where parasitic gaps are acceptable. The fact that parasitic gaps are constructed incrementallyin language processing presents a challenge for attempts to explain subject islands as epiphenomena of constraints on language processing and also helps to resolve apparent conflicts in previous studies of the role of island constraints in parsing.
An end‐to‐end channel allocation scheme for a wireless mesh network

Co‐channel interference seriously influences the throughput of a wireless mesh network. This study proposes an end‐to‐end channel allocation scheme (EECAS) that extends the radio‐frequency‐slot method to minimize co‐channel interference. The EECAS first separates the transmission and reception of packets into two channels. This scheme can then classify the state of each radio‐frequency‐slot as transmitting, receiving, interfered, free, or parity. A node that initiates a communication session with a quality of service requirement can propagate a channel allocation request along the communication path to the destination. By checking the channel state, the EECAS can determine feasible radio‐frequency‐slot allocations for the end‐to‐end path. The simulation results in this study demonstrate that the proposed approach performs well in intra‐mesh and inter‐mesh communications, and it outperforms previous channel allocation schemes in end‐to‐end throughput. Copyright © 2013 John Wiley & Sons, Ltd.
Antiepileptic drugs—best practice guidelines for therapeutic drug monitoring: A position paper by the subcommission on therapeutic drug monitoring, ILAE Commission on Therapeutic Strategies

Although no randomized studies have demonstrated a positive impact of therapeutic drug monitoring (TDM) on clinical outcome in epilepsy, evidence from nonrandomized studies and everyday clinical experience does indicate that measuring serum concentrations of old and new generation antiepileptic drugs (AEDs) can have a valuable role in guiding patient management provided that concentrations are measured with a clear indication and are interpreted critically, taking into account the whole clinical context. Situations in which AED measurements are most likely to be of benefit include (1) when a person has attained the desired clinical outcome, to establish an individual therapeutic concentration which can be used at subsequent times to assess potential causes for a change in drug response; (2) as an aid in the diagnosis of clinical toxicity; (3) to assess compliance, particularly in patients with uncontrolled seizures or breakthrough seizures; (4) to guide dosage adjustment in situations associated with increased pharmacokinetic variability (e.g., children, the elderly, patients with associated diseases, drug formulation changes); (5) when a potentially important pharmacokinetic change is anticipated (e.g., in pregnancy, or when an interacting drug is added or removed); (6) to guide dose adjustments for AEDs with dose‐dependent pharmacokinetics, particularly phenytoin.
Application of Ecotoxicogenomics for Studying Endocrine Disruption in Vertebrates and Invertebrates

Chemicals released into the environment potentially disrupt the endocrine system in wild animals and humans. Developing organisms are particularly sensitive to estrogenic chemicals. Exposure to estrogens or estrogenic chemicals during critical periods of development induces persistent changes in both reproductive and nonreproductive organs, including persistent molecular alterations. Estrogen-responsive genes and critical developmental windows of various animal species, therefore, need to be identified for investigators to understand the molecular basis of estrogenic activity during embryonic development. For investigators to understand molecular mechanisms of toxicity in various species, toxicogenomics/ecotoxicogenomics, defined as the integration of genomics (transcriptomics, proteomics, metabolomics) into toxicology and ecotoxicology, need to be established as powerful tools for research. As the initial step toward using genomics to examine endocrine-disrupting chemicals, estrogen receptors and other steroid hormone receptors have been cloned in various species, including reptiles, amphibians, and fish, and alterations in the expression of these genes in response to chemicals were investigated. We are identifying estrogen-responsive genes in mouse reproductive tracts using cDNA microarrays and trying to establish microarray systems in the American alligator, roach, medaka, and water fleas (Daphnia magna). It is too early to define common estrogen-responsive genes in various animal species; however, toxicogenomics and ectotoxicogenomics provide powerful tools to help us understand the molecular mechanism of chemical toxicities in various animal species.
Expression of a GABAB - Receptor in Olfactory Sensory Neurons of Sensilla trichodea on the Male Antenna of the Moth Heliothis virescens

In the olfactory pathway of Drosophila, a GABAB receptor mediated presynaptic gain control mechanism at the first synapse between olfactory sensory neurons (OSNs) and projection neurons has been suggested to play a critical role in setting the sensitivity and detection range of the sensory system. To approach the question if such a mechanism may be realized in the pheromone recognition system of male moths in this study attempts were made to explore if moth's pheromone-responsive cells express a GABAB- receptor. Employing a combination of genome analysis, RT-PCR experiments and screening of an antennal cDNA library we have identified a cDNA which encodes the GABAB-R1 receptor of Heliothis virescens. Moreover, based on the HvirGABAB-R1 sequence we could predict a GABAB-R1 protein from genome sequences of the silkmoth Bombyx mori. To assess whether HvirGABAB-R1 is expressed in OSNs of male antenna we performed whole-mount in situ hybridization (WM-ISH) experiments. Several HvirGABAB-R1 positive cells were visualized under long sensilla trichodea, known to contain pheromone-responsive OSNs. In parallel it was shown that cells under long trichoid hairs were labelled with pheromone receptor specific probes. In addition, the HvirGABAB-R1 specific probe also labelled several cells under shorter olfactory sensilla, but never stained cells under mechanosensory/gustatory sensilla chaetica. Together, the results indicate that a GABAB receptor is expressed in pheromone-responsive OSNs of H. virescens and suggest a presynaptic gain control mechanism in the axon terminals of these cells.
Reading Aloud and Solving Simple Arithmetic Calculation Intervention (Learning Therapy) Improves Inhibition, Verbal Episodic Memory, Focus Attention and Processing Speed in Healthy Elderly People: Evidence from a Randomized Controlled Trial

Background: Previous reports have described that simple cognitive training using reading aloud and solving simple arithmetic calculations, so-called “learning therapy”, can improve executive functions and processing speed in the older adults. Nevertheless, it is not well-known whether learning therapy improve a wide range of cognitive functions or not. We investigated the beneficial effects of learning therapy on various cognitive functions in healthy older adults. Methods: We used a single-blinded intervention with two groups (learning therapy group: LT and waiting list control group: WL). Sixty-four elderly were randomly assigned to LT or WL. In LT, participants performed reading Japanese aloud and solving simple calculations training tasks for 6 months. WL did not participate in the intervention. We measured several cognitive functions before and after 6 months intervention periods. Results: Compared to WL, results revealed that LT improved inhibition performance in executive functions (Stroop: LT (Mean = 3.88) vs. WL (Mean = 1.22), adjusted p = 0.013 and reverse Stroop LT (Mean = 3.22) vs. WL (Mean = 1.59), adjusted p = 0.015), verbal episodic memory (Logical Memory (LM): LT (Mean = 4.59) vs. WL (Mean = 2.47), adjusted p = 0.015), focus attention (D-CAT: LT (Mean = 2.09) vs. WL (Mean = −0.59), adjusted p = 0.010) and processing speed compared to the WL control group (digit symbol coding: LT (Mean = 5.00) vs. WL (Mean = 1.13), adjusted p = 0.015 and Symbol Search (SS): LT (Mean = 3.47) vs. WL (Mean = 1.81), adjusted p = 0.014). Discussion: This randomized controlled trial (RCT) can be showed the benefit of LT on inhibition of executive functions, verbal episodic memory, focus attention and processing speed in healthy elderly people. Our results were discussed under overlapping hypothesis.
Determination of Optimal Parameters for the Chebyshev-Gegenbauer Reconstruction Method

We propose a new strategy for choosing the optimal parameters for the Gegenbauer reconstruction method based on Chebyshev spectral coefficients under different assumptions on the smoothness of the function f. These parameters are optimal in the sense that the bounds on the truncation and regularization errors were forced to be equal. This strategy is independent on the number of terms N in the Chebyshev expansion of the function f and guarantees exponential convergence as $N\rightarrow \infty$ of the Gegenbauer series to f on the intervals of smoothness. The effectiveness of this strategy and exponential convergence are confirmed by numerical examples for functions with varying degrees of smoothness.
The Predictive Influence of Extraversion and Neuroticism on Life Satisfaction Judgments of Adults in Ado -Ekiti, Nigeria

This study investigated the predictive influence of personality factors, extraversion and neuroticism, on life satisfaction judgements of educated adults in different age categories. Employing a sample of 544 participants of educated adults in the age cohorts of 20 – 39 years (male = 75, female = 201), 40 – 59 years (male = 63. female = 116), and 60+ years (male = 49, female = 40), selected through a multi-stage sampling, and administering on them Life Satisfaction Index, Big Five Inventory, and a Personal Data Inventory, analyses of data using Simple Linear Regression revealed that (i) extraversion predicted life satisfaction among educated adults in age category 20-39 years only, and not in adults in age categories 40-59 years and 60+ years. (ii) neuroticism predicted life satisfaction among educated adults in all the different age categories. Based on the finding, recommendations were made to Nigerian Governments and many relevant others and agencies, in the lives of Nigerian adults to use the opportunity of the knowledge of the importance of these factors in life satisfaction judgements among adults to help Nigerian adults feel satisfied with their lives.
PERBEDAAN RADIOPASITAS ANTARA BAHAN OBTURASI SEALER BERBAHAN DASAR KALSIUM HIDROKSIDA DAN EPOKSI RESIN DENGAN TEKNIK RADIOGRAFI CONE BEAM COMPUTED TOMOGRAPHY (CBCT)

Penyakit gigi dan mulut di Indonesia terutama karies masih banyak diderita. Salah satu cabang kedokteran gigi yang menangani perawatan gigi dikenal dengan perawatan Endodontik. Perawatan saluran akar merupakan bagian dari perawatan endodontik yang salah satu prosedurnya terdiri dari obturasi saluran akar. Bahan obturasi saluran akar yang digunakan hingga saat ini adalah gutta percha yang dalam aplikasinya harus dikombinasikan dengan sealer saluran akar. Syarat-syarat sealer salah satunya adalah tingkat radiopasitas. Penelitian ini bertujuan untuk mengetahui perbedaan radiopasitas antara sealer obturasi berbahan dasar kalsium hidroksida dengan sealer berbahan dasar epoksi resin pada teknik CBCT. Jenis penelitian ini adalah penelitian eksperimental laboratorium.Analisa statistik menggunakan independent sample t-tes diperoleh nilai p= 0,000<0,05 artinya terdapat perbedaan radiopasitas antara sealer obturasi berbahan dasar kalsium hidroksida dengan sealer berbahan dasar epoksi resin pada teknik CBCT secara signifikan dimana nilai radiopasitas tertinggi terdapat pada sampel sealer berbahan dasar epoksi resin
XML Query Recommendation Based On Association Rules

With the growing popularity of XML technology, developing effective and efficient query techniques of XML data becomes an important problem. In this paper, we introduce a query recommendation technique, which is able to provide both result recommendation and query input recommendation for XML queries, to help users to find useful information more efficiently. Ln our approach, we mine association rules from XML documents to discover related XML fragments and recommend to the user the possibly interesting results, which are however not included in the original query results. In order to restrict the number of discovered rules and to discard uninteresting rules, our system allows users to specify constraints on the generated association rules. In addition to the recommendation of results, our system also provides potentially relevant XML queries to help users to find the desired content rapidly. Our experimental results show that our methods can provide recommendations for both query results and query input effectively.
Ieee Geoscience and Remote Sensing Letters, Statistical Descriptors of Ocean Regimes from the Geometric Regularity of Sst Observations

In this paper we evaluate to which extent the activity of ocean fronts can be retrieved from the geometric regularity of ocean tracer observations. Applied to sea surface temperature (SST), we propose a method for the characterization of this geometric regularity from curvature-based statistics along temperature level-lines in front regions. To assess the effectiveness of the proposed descriptors, we used 6 years (from 2003 to 2008) of daily SST observations of the regions of Agulhas in the South of Africa, and of Malvinas off the southern Brazilian coast. These experiments stress the relevance of geometric regularity features of tracer observation at ocean surface to characterize seasonal variations in ocean regimes.
Incorporating Planning into BDI Systems

Many architectures of autonomous agent have been proposed throughout AI research. The most common architectures, BDI, are procedural in that they do no planning, seriously curtailing an agent's ability to cope with unforeseen events. In this paper, we explore the relationship between propositional planning systems and the process of means-ends reasoning used by BDI agents and define a mapping from BDI mental states to propositional planning problems and from propositional plans back to mental states. In order to test the viability of such a mapping, we have implemented it in an extension of a BDI agent model through the use of Graphplan as the propositional planning algorithm. The implemented prototype was applied to model a case study of an agent controlled production cell.
Refolding human serum albumin at relatively high protein concentration.

The conditions for refolding reduced and denatured human serum albumin (HSA) were investigated with a view to maximising the yield of native monomeric albumin. Refolding by dialysis was found to be preferable to dilution as a means of chaotrope (urea) and reductant (2-mercaptoethanol) removal. Dialysis of denatured HSA solutions containing 4-8 M urea and 14 mM 2-mercaptoethanol at pH 10.0 was found to be optimal for HSA refolding. The yield of monomeric HSA was maximal (94%) for dialysis in the presence of EDTA (1 mM) and sodium palmitate (20 microM). Using this protocol it was possible to refold HSA at concentrations in excess of 5 mg.ml-1 whilst maintaining a high recovery of native monomer. These results represent a considerable improvement on established methods of HSA refolding.
Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing

A key player in translation initiation is eIF4E, the mRNA 5′ cap-binding protein. 4E-Transporter (4E-T) is a recently characterized eIF4E-binding protein, which regulates specific mRNAs in several developmental model systems. Here, we first investigated the role of its enrichment in P-bodies and eIF4E-binding in translational regulation in mammalian cells. Identification of the conserved C-terminal sequences that target 4E-T to P-bodies was enabled by comparison of vertebrate proteins with homologues in Drosophila (Cup and CG32016) and Caenorhabditis elegans by sequence and cellular distribution. In tether function assays, 4E-T represses bound mRNA translation, in a manner independent of these localization sequences, or of endogenous P-bodies. Quantitative polymerase chain reaction and northern blot analysis verified that bound mRNA remained intact and polyadenylated. Ectopic 4E-T reduces translation globally in a manner dependent on eIF4E binding its consensus Y30X4Lϕ site. In contrast, tethered 4E-T continued to repress translation when eIF4E-binding was prevented by mutagenesis of YX4Lϕ, and modestly enhanced the decay of bound mRNA, compared with wild-type 4E-T, mediated by increased binding of CNOT1/7 deadenylase subunits. As depleting 4E-T from HeLa cells increased steady-state translation, in part due to relief of microRNA-mediated silencing, this work demonstrates the conserved yet unconventional mechanism of 4E-T silencing of particular subsets of mRNAs.
Course Recommendation by Improving BM25 to Identity Students' Different Levels of Interests in Courses

BM25 is a well known ranking method to measure the relevance of the matching documents to a query.BM25 ranking functions can be used in recommendation when the query is transferred to represent users’ interest. However, in this scenario,terms in the query need to be handled differently because they may come from sources that users have different levels of interests in. For example, some terms come from areas that user has much interest in; some terms come from areas that user has some interest in.Course recommendation, which is very useful in university portals, can make use of students’ course selection history. Course recommendation should consider that students have different levels of interests in different courses they selected. This paper proposes an improvement on BM25 to fulfill this requirement.Other than course recommendation, the improvement can be used in information systems of other areas, for example, financial news recommendation. The experimental result shows the validity and usability of the proposed method.