Miracle-dz/UniFAR-FTUs huggingface dataset:
A new subtropical-temperate brooding echinoid with no marsupium: the first Mediterranean and the last European Temnopleuridae from the Early Pleistocene of Italy

The regular echinoid Placentinechinus davolii gen. et sp. nov. is described from eight Early Pleistocene (Gelasian–Calabrian) sites in north and south Italy. It is the most recent record known for the family Temnopleuridae in the European domain and the first in the Mediterranean area. A review of temnopleurid palaeobiogeography, and morphological comparisons, suggest that P. davolii was derived from a marsupiate brooder that lived in the north-east Atlantic during Messinian–middle Gelasian time. This indicates a brooding reproductive strategy for P. davolii, although it does not bear any evidence of a marsupium. The stratigraphy and climatic setting inferred for the study sites indicate that Placentinechinus represents a significant southward shift of the European Temnopleuridae, triggered by a progressive cooling that changed the Mediterranean climate from tropical to subtropical-temperate. A further climatic deterioration, perhaps that at about 0.8 Ma with the onset of the 100 kyr-controlled Ice Ages, caused its extinction. The palaeoecology of Placentinechinus, as deduced from palaeoenvironmental reconstructions of the study sites, indicates that it lived in shoreface to shallow offshore, moderately agitated waters, often together with the scallop-polychaete Aequipecten-Ditrupa association. It tolerated more or less marked conditions of turbidity, but flourished in trophically well-structured palaeocommunities. The main morphological characters distinguishing Placentinechinus are the very depressed test and the extremely large apical opening, up to 82% of the test diameter, which is so far the largest known for an adult echinacean echinoid. The statistically exhaustive morphometric data collected from more than 100 Placentinechinus tests indicate that inferring sexual dimorphism for Temnopleuridea echinoids based only on the apical disc width could be misleading. For P. davolii, both climate and environmental stability, in terms of sedimentation rate and nutrient supply, may have been concurrent drivers of its evolutionary history. http://zoobank.org/urn:lsid:zoobank.org:pub:EBB96C8C-943D-4253-82FE-09B9132591E7
A COMPLETE FRAMEWORK OPERATING SPATIALLY-ORIENTED RTI IN A 3D/2D CULTURAL HERITAGE DOCUMENTATION AND ANALYSIS TOOL

Abstract. Close-Range Photogrammetry (CRP) and Reflectance Transformation Imaging (RTI) are two of the most used image-based techniques when documenting and analyzing Cultural Heritage (CH) objects. Nevertheless, their potential impact in supporting study and analysis of conservation status of CH assets is reduced as they remain mostly applied and analyzed separately. This is mostly because we miss easy-to-use tools for of a spatial registration of multimodal data and features for joint visualisation gaps. The aim of this paper is to describe a complete framework for an effective data fusion and to present a user friendly viewer enabling the joint visual analysis of 2D/3D data and RTI images. This contribution is framed by the on-going implementation of automatic multimodal registration (3D, 2D RGB and RTI) into a collaborative web platform (AIOLI) enabling the management of hybrid representations through an intuitive visualization framework and also supporting semantic enrichment through spatialized 2D/3D annotations.
Critical research gaps in treating growth faltering in infants under 6 months: A systematic review and meta-analysis

In 2020, 149.2 million children worldwide under 5 years suffered from stunting, and 45.4 million experienced wasting. Many infants are born already stunted, while others are at high risk for growth faltering early after birth. Growth faltering is linked to transgenerational impacts of poverty and marginalization. Few interventions address growth faltering in infants under 6 months, despite a likely increasing prevalence due to the negative global economic impacts of the COVID-19 pandemic. Breastfeeding is a critical intervention to alleviate malnutrition and improve child health outcomes, but rarely receives adequate attention in growth faltering interventions. A systematic review and meta-analysis were undertaken to identify and evaluate interventions addressing growth faltering among infants under 6 months that employed supplemental milks. The review was carried out following guidelines from the USA National Academy of Medicine. A total of 10,405 references were identified, and after deduplication 7390 studies were screened for eligibility. Of these, 227 were assessed for full text eligibility and relevance. Two randomized controlled trials were ultimately included, which differed in inclusion criteria and methodology and had few shared outcomes. Both studies had small sample sizes, high attrition and high risk of bias. A Bangladeshi study (n = 153) found significantly higher rates of weight gain for F-100 and diluted F-100 (DF-100) compared with infant formula (IF), while a DRC trial (n = 146) did not find statistically significant differences in rate of weight gain for DF-100 compared with IF offered in the context of broader lactation and relactation support. The meta-analysis of rate of weight gain showed no statistical difference and some evidence of moderate heterogeneity. Few interventions address growth faltering among infants under 6 months. These studies have limited generalizability and have not comprehensively supported lactation. Greater investment is necessary to accelerate research that addresses growth faltering following a new research framework that calls for comprehensive lactation support.
MITOGENOME REPORT The complete mitochondrial genome of the red-footed tortoise ( Chelonoidis carbonarius Spix 1824)

In this study, we report the complete mitochondrial genome of the red-footed tortoise ( Chelonoidis car-bonarius ). The compete mitochondrial genome of C. carbonarius is 16,639bp in length, including 13 protein-coding genes, 22 tRNA genes, and two rRNA genes. The mitogenome was deposited in NCBI GenBank under the accession number OQ789392. Furthermore, we also constructed a phylogenetic tree of Chelonoidis using eight species. These results will aid the conservation of Chelonoidis from the perspective of genetic evolution.
Information-theoretic analysis of a model of CAR-4–1BB-mediated NFκB activation

Systems biology utilizes computational approaches to examine an array of biological processes, such as cell signaling, metabolomics and pharmacology. This includes mathematical modeling of CAR T cells, a modality of cancer therapy by which genetically engineered immune cells recognize and combat a cancerous target. While successful against hematologic malignancies, CAR T cells have shown limited success against other cancer types. Thus, more research is needed to understand their mechanisms of action and leverage their full potential. In our work, we set out to apply information theory on a mathematical model of cell signaling of CAR-mediated activation following antigen encounter. First, we estimated channel capacity for CAR-4-1BB-mediated NFκB signal transduction. Next, we evaluated the pathway’s ability to distinguish contrasting “low” and “high” antigen concentration levels, depending on the amount of intrinsic noise. Finally, we assessed the fidelity by which NFκB activation reflects the encountered antigen concentration, depending on the prevalence of antigen-positive targets in tumor population. We found that in most scenarios, fold change in the nuclear concentration of NFκB carries a higher channel capacity for the pathway than NFκB’s absolute response. Additionally, we found that most errors in transducing the antigen signal through the pathway skew towards underestimating the concentration of encountered antigen. Finally, we found that disabling IKKβ deactivation could increase signaling fidelity against targets with antigen-negative cells. Our information-theoretic analysis of signal transduction can provide novel perspectives on biological signaling, as well as enable a more informed path to cell engineering.
Self-assembly of DNA parallel double-crossover motifs

DNA double-crossover motifs, including parallel and antiparallel crossovers, serve as the structural foundation for the creation of diverse nanostructures and dynamic devices in DNA nanotechnology. Parallel crossover motifs have unique advantages over the widely used antiparallel crossover design but have not developed as substantially due to the difficulties in assembly. Here we created 29 designs of parallel double-crossover motifs varying in hybridization pathways, central domain lengths, and crossover locations to investigate their assembly mechanism. Arrays were successfully formed in four distinct designs, and large tubular structures were obtained in seven designs with predefined pathways and central domains appoximately 16 nucleotides in length. The nanotubes obtained from parallel crossover design showed improved nuclease resistance than the ones from the antiparallel counterpart design. Overall, our study provides a basis for the development of generalized assembly rules of DNA parallel crossover systems and opens new opportunities for their potential use in biological systems.
Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling

ABSTRACT Plants, as sessile organisms, are subjected to diverse abiotic stresses, including salinity, desiccation, metal toxicity, thermal fluctuations, and hypoxia at different phases of plant growth. Plants can activate messenger molecules to initiate a signaling cascade of response toward environmental stresses that results in either cell death or plant acclimation. Nitric oxide (NO) is a small gaseous redox-active molecule that exhibits a plethora of physiological functions in growth, development, flowering, senescence, stomata closure and responses to environmental stresses. It can also facilitate alteration in protein function and reprogram the gene profiling by direct or indirect interaction with different target molecules. The bioactivity of NO can be manifested through different redox-based protein modifications including S-nitrosylation, protein nitration, and metal nitrosylation in plants. Although there has been considerable progress in the role of NO in regulating stress signaling, still the physiological mechanisms regarding the abiotic stress tolerance in plants remain unclear. This review summarizes recent advances in understanding the emerging knowledge regarding NO function in plant tolerance against abiotic stresses. The manuscript also highlighted the importance of NO as an abiotic stress modulator and developed a rational design for crop cultivation under a stress environment.
Natural transformation-specific DprA coordinate DNA double-strand break repair pathways in heavily irradiated D. radiodurans

ABSTRACT Deinococcus radiodurans exhibits remarkable survival under extreme conditions, including ionizing radiation, desiccation, and various DNA-damaging agents. It employs unique repair mechanisms, such as single-strand annealing (SSA) and extended synthesis-dependent strand annealing (ESDSA), to efficiently restore damaged genome. In this study, we investigate the role of the natural transformation-specific protein DprA in DNA repair pathways following acute gamma radiation exposure. Our findings demonstrate that the absence of DprA leads to rapid repair of gamma radiation-induced DNA double-strand breaks primarily occur through SSA repair pathway. Additionally, our findings suggest that the DprA protein may hinder both the SSA and ESDSA repair pathways, albeit in distinct manners. Overall, our results highlight the crucial function of DprA in the selection between SSA and ESDSA pathways for DNA repair in heavily irradiated D. radiodurans. IMPORTANCE Deinococcus radiodurans exhibits an extraordinary ability to endure and thrive in extreme environments, including exposure to radiation, desiccation, and damaging chemicals, as well as intense UV radiation. The bacterium has evolved highly efficient repair mechanisms capable of rapidly mending hundreds of DNA fragments in its genome. Our research indicates that natural transformation (NT)-specific dprA genes play a pivotal role in regulating DNA repair in response to radiation. Remarkably, we found that DprA is instrumental in selecting DNA double-strand break repair pathways, a novel function that has not been reported before. This unique regulatory mechanism highlights the indispensable role of DprA beyond its native function in NT and underscores its ubiquitous presence across various bacterial species, regardless of their NT proficiency. These findings shed new light on the resilience and adaptability of Deinococcus radiodurans, opening avenues for further exploration into its exceptional survival strategies. Deinococcus radiodurans exhibits an extraordinary ability to endure and thrive in extreme environments, including exposure to radiation, desiccation, and damaging chemicals, as well as intense UV radiation. The bacterium has evolved highly efficient repair mechanisms capable of rapidly mending hundreds of DNA fragments in its genome. Our research indicates that natural transformation (NT)-specific dprA genes play a pivotal role in regulating DNA repair in response to radiation. Remarkably, we found that DprA is instrumental in selecting DNA double-strand break repair pathways, a novel function that has not been reported before. This unique regulatory mechanism highlights the indispensable role of DprA beyond its native function in NT and underscores its ubiquitous presence across various bacterial species, regardless of their NT proficiency. These findings shed new light on the resilience and adaptability of Deinococcus radiodurans, opening avenues for further exploration into its exceptional survival strategies.
Pattern Recognition Methods for Reduction of Human-Wildlife Conflicts

Description: Conflicts between human activities and wildlife are an increasing problem, and in many parts of the world damage caused by wildlife creates significant economic challenges to human communities. Methods for reducing human-wildlife conflicts are either ineffective, time consuming or costly. Both lethal and non-lethal techniques are used for reduction of human-wildlife conflicts. However, the use of lethal methods is often controversial, as there is a public desire to co-exist with wildlife.
Nurses quietly quit their job more often than other healthcare workers: An alarming issue for healthcare services.

AIM To assess the level of quiet quitting among healthcare workers (HCWs) and identify possible differences between nurses, physicians, and other HCWs. We investigated the impact of sociodemographic variables, job burnout, and job satisfaction on quiet quitting levels. BACKGROUND The quiet-quitting phenomenon is not new but has been frequently discussed during the COVID-19 pandemic. Interestingly, the level of quiet quitting among HCWs has not been measured yet. METHODS We conducted a cross-sectional study with a convenience sample. We measured sociodemographic variables, job burnout, job satisfaction, and quiet quitting. We adhered to STROBE guidelines for cross-sectional studies. FINDINGS Among our sample, 67.4% of nurses were quiet quitters, while the prevalence of quiet quitting for physicians and other HCWs was 53.8% and 40.3%, respectively. Multivariable linear regression analysis identified that the levels of quiet quitting were higher among nurses than physicians and other HCWs. Moreover, greater job burnout contributed more to quiet quitting, while less satisfaction implied more quiet quitting. HCWs who work in shifts and those working in the private sector experienced higher levels of quiet quitting. DISCUSSION More than half of our HCWs were described as quit quitters. Levels of quiet quitting were higher among nurses. Job burnout and job dissatisfaction were associated with higher levels of quiet quitting. IMPLICATIONS FOR NURSING PRACTICE AND NURSING POLICY Measurement of quiet quitting and identification of risk factors are essential to prevent or reduce quiet quitting levels among HCWs. Our study provides information on this field helping managers and organizations to identify quiet quitters within HCWs. Policymakers and managers should develop and implement interventions both at an organizational level and at an individual level.
Spatial Distribution of Air Dose Rate in Grazing Grassland

A field study was carried out to determine the spatial distribution of air dose rate on grazed grassland after the earthquake on 11 March, 2011 in the Northwest Pacific of Northeastern Japan. Data on air dose rates (μSv h-1) were collected from Ichinoseki, south of Iwate Prefecture, Japan. Air dose rates were collected from each of 1 m interval of 12 ×12 m^2 site (L-site). At the center of Lsite, 1.2 ×1.2 m^2 site (S-site) was located. One hundred and forty four (144) equal spaced quadrats were defined in the S-site. Again, air dose rates were collected from central point of each of the quadrat. Moran’s I, a measure of autocorrelation was used to test the spatial heterogeneity of air dose rate on grazed grassland. Autocorrelation in S-site area was significantly higher than L-site area. Air dose rate did not show significant autocorrelation at any spatial lag in L-site. In S-site, air dose rate level showed significant autocorrelation in twelve of sixteen spatial lag. Autocorrelograms and Moran’s scatterplot showed that air dose rate was frequently and positively spatially correlated at distance less than 0.1 m.
Wind farm characterization and control using coherent Doppler lidar

Wind measurements are fundamental inputs for the evaluation of potential energy yield and performance of wind farms. Three-dimensional scanning coherent Doppler lidar (CDL) may provide a new basis for wind farm site selection, design, and control. In this research, CDL measurements obtained from multiple wind energy developments are analyzed and a novel wind farm control approach has been modeled. The possibility of using lidar measurements to more fully characterize the wind field is discussed, specifically, terrain effects, spatial variation of winds, power density, and the effect of shear at different layers within the rotor swept area. Various vector retrieval methods have been applied to the lidar data, and results are presented on an elevated terrain-following surface at hub height. The vector retrieval estimates are compared with tower measurements, after interpolation to the appropriate level. CDL data is used to estimate the spatial power density at hub height. Since CDL can measure winds at different vertical levels, an approach for estimating wind power density over the wind turbine rotor-swept area is explored. Sample optimized layouts of wind farm using lidar data and global optimization algorithms, accounting for wake interaction effects, have been explored. An approach to evaluate spatial wind speed and direction estimates from a standard nested Coupled Ocean and Atmosphere Mesoscale Prediction System (COAMPS) model and CDL is presented. The magnitude of spatial difference between observations and simulation for wind energy assessment is researched. Diurnal effects and ramp events as estimated by CDL and COAMPS were inter-compared. Novel wind farm control based on incoming winds and direction input from CDL's is developed. Both yaw and pitch control using scanning CDL for efficient wind farm control is analyzed. The wind farm control optimizes power production and reduces loads on wind turbines for various lidar wind speed and direction inputs, accounting for wind farm wake losses and wind speed evolution. Several wind farm control configurations were developed, for enhanced integrability into the electrical grid. Finally, the value proposition of CDL for a wind farm development, based on uncertainty reduction and return of investment is analyzed.
Update on therapeutic use of levothyroxine for the management of hypothyroidism during pregnancy

Hypothyroidism is a relatively common finding during pregnancy. This may be due either to the presence of existing thyroid disease and/or to the increased demands that pregnancy places the thyroid gland to provide thyroid hormones for the mother and the developing fetus. There is no doubt that overt hypothyroidism is associated strongly with adverse pregnancy outcomes, including miscarriage. Meta-analyses show that thyroid hormone replacement with levothyroxine (LT4) reduces the risk of adverse pregnancy outcomes in the setting of overt hypothyroidism. Accordingly, management guidelines in this area are unanimous in recommending intervention with to control the level of thyrotropin (TSH) to below 2.5 μIU/mL. The evidence for an adverse impact of subclinical hypothyroidism (SCH) on pregnancy outcomes is less clear, although meta-analyses suggest that SCH reduces the chance of a successful pregnancy outcome. Guidelines also support intervention for some patients with SCH, particularly where TSH is high (>10 μIU/mL), or where TSH is above its trimester-specific reference range in a woman with thyroid autoimmunity (giving LT4 to euthyroid women with thyroid autoimmunity is not supported). Real-world evidence suggests that hypothyroidism in pregnancy is often overlooked or that LT4 is not given appropriately to gain tight control of TSH. More research is needed to identify the barriers to optimal thyroid care with LT4 at this crucial time.
Adapting hip arthroplasty practices during the COVID-19 pandemic: Assessing the impact of outpatient care sudden increase on early complications and clinical outcomes

Introduction: The COVID-19 pandemic has significantly affected access to timely care for patients with hip osteoarthritis requiring total hip replacement (THR). This study aimed to assess the changes in surgical activity, outpatient treatment, length of stay (LOS), discharge destinations, readmission rates, clinical outcomes, and patient satisfaction before and after the pandemic at our institution. Materials and methods: This retrospective study encompassed patients undergoing primary THR through the direct anterior approach at a single university hospital. Data on demographic characteristics, surgical technique, perioperative management, LOS, discharge destinations, complications, and clinical outcomes were collected. Furthermore, a comparative analysis between the pre-pandemic (2019) and post-pandemic (2022) periods was conducted. Results: There was a 14% increase in surgical activity post-pandemic, with 214 patients undergoing surgery in 2019 versus 284 in 2022. The percentage of patients managed as outpatients significantly increased from 0.5% in 2019 to 29.6% in 2022 (p < 0.001). LOS decreased from 2.7 ± 1 [0–8] days to 1.4 ± 1.1 [0–12] days (p < 0.001), and the rate of discharge to rehabilitation centres declined from 21.5% to 8.8% (p < 0.001). No significant increase in the readmission rates was observed (1.4% in both periods). At two months postoperatively, the mean HHS and satisfaction rates were comparable between the two groups (p = 1 and p = 0.73, respectively). Discussion: Despite the challenges posed by the COVID-19 pandemic, surgical activity at our institution demonstrated an increase compared to the pre-pandemic levels by expanding outpatient care, reducing LOS, and increasing rates of home discharges. Importantly, these changes did not adversely affect rehospitalization rates or early clinical outcomes. Level of evidence: IV