Miracle-dz/UniFAR-FTUs huggingface dataset:
DUSP15 expression is reduced in the hippocampus of Myrf knock-out mice but attention and object recognition memory remain intact

The atypical protein tyrosine phosphatase enzyme, dual-specificity phosphate 15 (DUSP15) is thought to be activated by myelin regulatory factor (MyRF) and to have a role in oligodendrocyte differentiation. Here, we assess whether Dusp15 is reduced in the hippocampus of mice with conditional knock-out of Myrf in oligodendrocyte precursor cells. Using quantitative polymerase chain reaction (qPCR) we found that Dusp15 expression was indeed lower in these mice. Alterations in myelin have been associated with Alzheimer’s disease (AD), autism spectrum disorder (ASD) and attention deficit/hyperactivity disorder (ADHD). Symptoms of these disorders can include impairments of object recognition and attention. We, therefore tested the mice in the object recognition task (ORT) and 5-choice serial reaction time task (5CSRTT). However, we did not find behavioural impairments indicating that attentional abilities and object recognition are not impacted by reduced oligodendrogenesis and hippocampal Dusp15 expression. Gaining insight into the role of newly formed oligodendrocytes and Dusp15 expression is helpful for the development of well targeted treatments for myelin dysregulation.
Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids

Significance The kidneys have highly complex architectures and functions. Advanced models are therefore required to push research into human kidney development, function, disease, and regeneration forward. Organoids from induced pluripotent stem cells and tubuloids grown from adult stem/progenitor cells are two novel models that have proven valuable for these purposes. These models each have their specific advantages and uses. Here, both protocols are combined to generate ‘iPSCod tubuloids’, a different laboratory model of the kidney with complementary advantages to both organoids and tubuloids. iPSCod tubuloids are easily handled and display key kidney functions such as waste excretion and salt reabsorption. We expect that this approach will prove useful for future research into human kidney function and disease.
Comparison of compensatory shoulder movements, functionality and satisfaction in transradial amputees fitted with two prosthetic myoelectric hooks

The functionalities of myoelectric hooks, such as whether they allow wrist movements, as well as the volume and design of the devices, may impact how fitted transradial amputees use their upper limbs. The aim of the current study was to compare two prosthetic myoelectric hooks in terms of compensatory shoulder movements, functionality and user satisfaction. This monocentric, randomized, controlled, cross-over trial evaluated eight transradial amputees fitted with two prosthetic myoelectric hooks, the Greifer and the Axon-Hook, during two consecutive periods. At the end of each period, shoulder abduction (mean and percentage of time with shoulder abduction > 60°) and manual dexterity were assessed using the Box and Blocks Test (BBT) on both sides, and satisfaction was assessed with the Evaluation of Satisfaction with Assistive Technology questionnaire. For each patient, data obtained with the BBT on the amputated side were compared with those obtained on the non-amputated side. Shoulder abduction was significantly higher with the Greifer (60.9°± 20.3°, p = 0.03) than with the Axon-Hook (39.8°± 16.9°) and also than with the NA side (37.6 ± 19.4°, p = 0.02). Shoulder abduction on the NA side (37.6 ± 19.4°) was close to that of the Axon-Hook (39.8°± 16.9°). The percentage of time spent with shoulder abduction > 60° during the BBT was higher with the Greifer than with the Axon-Hook or with the NA side (53.3 ± 34.4%, 17.6 ± 27.0% and 18.4 ± 34.9%, respectively), but the differences were not significant (p = 0.15). A significant strong negative correlation was found between shoulder abduction and wrist position with the Axon-Hook (r = -0.86; p < 0.01), but not with the Greifer. Manual dexterity and satisfaction did not differ significantly between the two devices. These results revealed compensatory movements, such as shoulder abduction in transradial amputees equipped with hooks, themselves influenced by the prosthetic device settings.
Psychosocial, Medical, and Demographic Variables Associated with Parent Mealtime Behavior in Young Children Recently Diagnosed with Type 1 Diabetes

Abstract: Objective: Managing young children's mealtime concerns can be challenging after type 1 diabetes (T1D) diagnosis because of developmental factors and diabetes management demands. To identify potential intervention targets, we evaluated medical, psychosocial, and demographic factors in relation to parents' engagement in problem mealtime behaviors (e.g., pressure to eat, restriction). Method: Parents (N = 157) of young children (age 1–6 years) reported on psychosocial variables (parent fear of hypoglycemia, family functioning, parent problem solving, and parents' problem mealtime behavior frequency and perceptions of being problematic) within 2 months after T1D diagnosis. Hierarchical regression analyses examined associations among psychosocial variables, demographics (child sex, parent race/ethnicity), child continuous glucose monitor (CGM) use, and parents' problem mealtime behaviors. Results: Parents of children using CGMs reported parents' mealtime behaviors as more problematic than nonusers, but there were no differences for other medical or demographic variables. Models predicting parents' problem mealtime behavior frequency and problem perceptions that included psychosocial variables, demographic variables, and CGM use led to significant R2 of 0.14 and 0.16, respectively. CGM use and parent problem solving were significantly associated with parent mealtime behaviors being perceived as more problematic. Conclusion: Shortly after T1D diagnosis in young children, medical and parent psychosocial factors related to how frequently parents engaged in problem mealtime behaviors and the degree to which parents perceived them as problematic. Other factors may further explain the complexities of mealtime management. Considering parents' problem-solving skills and child treatment regimens may help guide interventions targeting mealtime challenges during the new diagnosis period.
A modified training scheme for SOFM to cluster multispectral images

In this paper, we propose modifications to Kohonen's Self-Organising Feature Map (SOFM) to achieve faster convergence specifically with respect to multispectral images. First, the raw image is pre-processed using data reduction technique to obtain reduced data set and then Condensed Nearest Neighbour (CNN) rule is applied to yield standard subset of samples. The samples in the standard subset are used to find the Best Matching Unit (BMU) and the samples in the reduced data set are used to update BMU and its neighbouring neurons. The SOFM is tested on: synthetic image data set and Harangi 1991, 1992 image data sets. Results are compared with conventional SOFM.
Evidence-Based Patient Selection in Headache Surgery: Impact of Preoperative Radiofrequency Ablation on Surgical Outcomes

Background: Radiofrequency ablation (RFA) of the greater occipital nerve (GON) is a minimally invasive treatment option commonly used in patients with occipital neuralgia. Patients who undergo occipital surgery for headaches after failed RFA treatment present a unique opportunity to evaluate RFA-treated occipital nerves and determine the impact on headache surgery outcomes. Methods: Of 115 patients who underwent headache surgery at the occipital site, 29 had a history of RFA treatment. Migraine Headache Index, Pain Self- Efficacy Questionnaire, and Pain Health Questionnaire-2 outcome scores were recorded preoperatively and at follow-up visits. Intraoperative macroscopic nerve damage and surgical outcomes were compared between RFA-treated and non–RFA-treated patients. Results: RFA-treated patients had a higher rate of macroscopic nerve damage (45%) than non–RFA-treated patients (24%) (P = 0.03), and they were significantly more likely to require a second operation at the site of primary decompression (27.6% versus 5.8%; P = 0.001) and GON transection (13.8% versus 3.5%; P = 0.04). Outcome scores at the last follow-up visit showed no statistically significant difference between RFA-treated and non–RFA-treated patients (P = 0.96). Conclusions: RFA-treated patients can ultimately achieve outcomes that are not significantly different from non–RFA-treated patients in occipital headache surgery. However, a higher number of secondary operations at the site of primary decompression and nerve transection are required to treat refractory symptoms. RFA-treated patients should be counseled about an increased risk of same-site surgery and possible GON transection to achieve acceptable outcomes. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, III.
Intraclass Correlations Values in International Development: Evidence Across Commonly Studied Domains in sub-Saharan Africa

The sharp increase in the number of experimental studies evaluating development programs raises the need for accurate intraclass correlations (ICC) to conduct power calculations so that researchers can design studies to detect meaningful effects with sufficient statistical power. The intraclass correlation is an important parameter for determining the statistical power of cluster-randomized trials. The parameter is rarely available to researchers planning a study until after the design is set and data are already collected. This paper takes an important step towards helping researchers working in sub-Saharan Africa to accurately estimate appropriate sample sizes for their clustered RCTs. The study draws from rich data sets in Kenya, Malawi, Zambia, and Zimbabwe. We present ICCs for a wide range of domains common for development research. Our results suggest that ICCs for commonly studied indicators in sub-Saharan Africa are lower than is often assumed in power calculations. ICC values are especially low for indicators associated with child nutrition and food security, suggesting that cluster-RCTs might be a viable design even when faced with limited budgets because sample size requirements are not much different from an individual random assignment design.
Unilateral renal atrophy 4 years after renal sympathetic denervation: a case report

Renal sympathetic denervation (RDN) carries a low risk of renal artery stenosis, and most cases occur within the first year. However, limited data are available on long-term incidence. Here, we present a case of a 68-year-old woman who underwent radiofrequency RDN for resistant hypertension. Ambulatory blood pressure improved following RDN and uptitration of antihypertensive drugs. Between year 3 and 4 after RDN, eGFR reduced from 87 to 50 ml/min per 1.73 m2. Ultrasound imaging revealed left renal atrophy, while subsequent magnetic resonance angiography showed a haemodynamically significant stenosis of the left renal artery. The patient remained in good clinical condition with stable blood pressure, while eGFR mildly deteriorated during a 6-year follow-up period. This case of renal artery stenosis occurred in a patient with multiple risk factors. A causal relationship to the RDN procedure cannot be confirmed nor ruled out. Long-term surveillance for adverse events should be considered in all RDN patients.
New Perspectives in Resistance Training Periodization: Mixed Session vs. Block Periodized Programs in Trained Men

Abstract Bartolomei, S, Zaniboni, F, Verzieri, N, and Hoffman, JR. New perspectives in resistance training periodization: mixed session vs. block periodized programs in trained men. J Strength Cond Res 37(3): 537–545, 2023—The purpose of this investigation was to compare the effects of 2 different periodized resistance training programs on maximal strength, power, and muscle architecture, in trained individuals. Twenty-two resistance-trained men were randomly assigned to either a mixed session training group (MSP; n = 11; age = 23.7 ± 2.6 years; body mass = 80.5 ± 9.8 kg; height = 175.5 ± 6.1 cm) or a block periodization group (BP; n = 11; age = 25.7 ± 4.6 years; body mass = 81.1 ± 10.7 kg; height = 176.8 ± 8.4 cm). Both training programs were 10 weeks in duration and were equated in volume. Each training session of the MSP focused on power, maximal strength, and hypertrophy, whereas each mesocycle within the BP focused on one of these components. Subjects were assessed for body composition, muscle architecture, maximal strength, and power. In addition, perceived training load, and training volume were calculated. Subjects in MSP experienced greater improvements in fat free mass (p = 0.021), muscle thickness of the pectoralis and vastus lateralis (p < 0.05), and a greater improvement in 1RM bench press (p < 0.001; +8.6% in MSP and +2% in BP) than in BP. By contrast, BP resulted in greater improvements in vertical jump (p = 0.022; +7.2%) compared with MSP (+1.2%). No significant differences were noted between the groups for perceived training load (p = 0.362) nor training volume (p = 0.169). Results of this study indicated that in a 10-week training study, MSP may enhance muscle hypertrophy and maximal strength to a greater extent than BP, with the same training volume and perceived training load. However, BP may be more effective for vertical jump improvement.
Influence of Fibrinogen Concentrate on Neonatal Clot Structure When Administered Ex Vivo After Cardiopulmonary Bypass

BACKGROUND: Bleeding is a serious complication of cardiopulmonary bypass (CPB) in neonates. Blood product transfusions are often needed to adequately restore hemostasis, but are associated with significant risks. Thus, neonates would benefit from other effective, and safe, hemostatic therapies. The use of fibrinogen concentrate (FC; RiaSTAP, CSL Behring, Marburg, Germany) is growing in popularity, but has not been adequately studied in neonates. Here, we characterize structural and degradation effects on the neonatal fibrin network when FC is added ex vivo to plasma obtained after CPB. METHODS: After approval by the institutional review board and parental consent, blood samples were collected from neonates undergoing cardiac surgery and centrifuged to yield platelet poor plasma. Clots were formed ex vivo from plasma obtained at several time points: (1) baseline, (2) immediately post-CPB, and (3) post-transfusion of cryoprecipitate. In addition, we utilized post-CPB plasma to construct the following conditions: (4) post-CPB +0.5 mg/mL FC, and (5) post-CPB +0.9 mg/mL FC. The resultant fibrin networks were imaged using confocal microscopy to analyze overall structure, fiber density, and alignment. Clots were also analyzed using a microfluidic degradation assay. Fibrinogen content was quantified for all plasma samples. RESULTS: The addition of 0.5 or 0.9 mg/mL FC to post-CPB samples significantly enhanced the median fiber density when compared to untreated post-CPB samples (post-CPB = 0.44 [interquartile range {IQR}: 0.36–0.52], post-CPB +0.5 mg/mL FC = 0.69 [0.56–0.77], post-CPB +0.9 mg/mL FC = 0.87 [0.59–0.96]; P = .01 and P = .006, respectively). The addition of 0.9 mg/mL FC to post-CPB samples resulted in a greater fiber density than that observed after the in vivo transfusion of cryoprecipitate (post-transfusion = 0.54 [0.45–0.77], post-CPB +0.9 mg/mL FC = 0.87 [0.59–0.96]; P = .002). Median fiber alignment did not differ significantly between post-CPB samples and samples treated with FC. Degradation rates were not statistically significant from baseline values with either 0.5 or 0.9 mg/mL FC. In addition, we found a significant correlation between the difference in the baseline and post-CPB fibrinogen concentration with patient age (P = .033) after controlling for weight. CONCLUSIONS: Our results show that clots formed ex vivo with clinically relevant doses of FC (0.9 mg/mL) display similar structural and degradation characteristics compared to the in vivo transfusion of cryoprecipitate. These findings suggest that FC is effective in restoring structural fibrin clot properties after CPB. Future studies after the administration of FC in vivo are needed to validate this hypothesis.
Did you donate? Talking about donations predicts compliance with solicitations for donations

Many forms of prosocial behaviour are highly institutionalized. They are facilitated by organizations that broker between donors and recipients. A highly effective tool that organizations use to elicit prosocial behaviour are solicitations for donations (e.g., of blood, time, or money). Using register and survey data on blood donations in the Netherlands, we examine to what extent compliance with these solicitations is predicted by being recruited via word of mouth (WOM) and talking about donations. Our model predicts that donors that are one unit higher on our measure of talking about donations (range = 1–4) have a 2.9 percentage points higher compliance with solicitations for donations. In addition, this association is stronger for novice donors. Our study demonstrates the social embedding of the donors’ decision-making processes about compliance. For practice, our results imply that organizations may increase their contributors’ communication about donations to increase the effectiveness of their solicitations.
Electron field emission enhancement of carbon nanowalls by plasma surface nitridation

Carbon nanowalls (CNWs) are two-dimensional carbon nanostructures consisting of stacked graphene sheets standing vertically on the substrate. The sharp edges of CNWs provide us with opportunities for applications as electron field emitter arrays. The effects of nitrogen plasma (NP) treatment on the surface of CNWs have been investigated in order to improve the electron field emission properties. The electron emission current from the edges of CNWs was drastically increased by the NP treatment. Morphological and chemical changes in the CNWs after the NP treatment were characterized using scanning electron microscopy, Raman spectroscopy, and x-ray photoelectron spectroscopy.
Reduced death anxiety as a mediator of the relationship between acute subjective effects of psychedelics and improved subjective well-being

Abstract Research over the past several decades suggests that meaningful psychedelic experiences can engender long-term effects on subjective wellbeing. However, less research has investigated the psychological mechanisms through which these effects may emerge. In the present study, participants (N = 201) completed an online survey that retrospectively measured the acute effects of a meaningful psychedelic experience, as well as changes in subjective well-being and death anxiety. Reductions in death anxiety significantly mediated the effects of mystical experience on satisfaction with life, positive affect, and negative affect. Reductions in death anxiety did not mediate any of the effects of psychological insight. Although correlational, the findings are consistent with the hypothesis that some of the benefits of psychedelic-induced mystical experiences on subjective well-being may emerge due to reductions in death anxiety. Nevertheless, further research is needed to establish a causal effect of reduced death anxiety on well-being in the context of psychedelic experiences.
Establishing a core outcomes set for massive transfusion: An Eastern Association for the Surgery of Trauma modified Delphi method consensus study

@notoriousRBGmd; @seberly; @jnahmias1; @DanteYeh; @MarkusZiesmann; @UABTrauma Modified Delphi method was used to achieve consensus on a Core Outcome Set for Massive Transfusion to improve consistency in reporting of outcome metrics in future trials. BACKGROUND The management of severe hemorrhage has changed significantly over recent decades, resulting in a heterogeneous description of diagnosis, treatment, and outcomes in the literature, which is not suitable for data pooling. Therefore, we sought to develop a core outcome set (COS) to help guide future massive transfusion (MT) research and overcome the challenge of heterogeneous outcomes reporting. METHODS Massive transfusion content experts were invited to participate in a modified Delphi study. For Round 1, participants submitted a list of proposed core outcomes. In subsequent rounds, panelists used a 9-point Likert scale to score proposed outcomes for importance. Core outcomes consensus was defined as >85% of scores receiving 7 to 9 and <15% of scores receiving 1 to 3. Feedback and aggregate data were shared between rounds. RESULTS From an initial panel of 16 experts, 12 (75%) completed three rounds of deliberation to reevaluate variables not achieving predefined consensus criteria. A total of 64 items were considered, with 4 items achieving consensus for inclusion as core outcomes: blood products received in the first 6 hours, 6-hour mortality, time to mortality, and 24-hour mortality. CONCLUSION Through an iterative survey consensus process, content experts have defined a COS to guide future MT research. This COS will be a valuable tool for researchers seeking to perform new MT research and will allow future trials to generate data that can be used in pooled analyses with enhanced statistical power. LEVEL OF EVIDENCE Diagnostic Test or Criteria; Level V.
Practice of reperfusion in patients with ST-segment elevation myocardial infarction in China: findings from the Improving Care for Cardiovascular Disease in China–Acute Coronary Syndrome project

Abstract Background: Reperfusion therapy is fundamental for ST-segment elevation myocardial infarction (STEMI). However, the details of contemporary practice and factors associated with reperfusion therapy in China are largely unknown. Therefore, this study aimed to explore reperfusion practice and its associated factors among hospitalized patients with STEMI in China. Methods: Patients with STEMI who were admitted to 159 tertiary hospitals from 30 provinces in China were included in the Improving Care for Cardiovascular Disease in China–Acute Coronary Syndrome project from November 2014 to December 2019. The associations of the characteristics of patients and hospitals with reperfusion were examined using hierarchical logistic regression. The associations between therapies and in-hospital major adverse cardiovascular events were examined with a mixed effects Cox regression model. Results: Among the 59,447 patients, 37,485 (63.1%) underwent reperfusion, including 4556 (7.7%) receiving fibrinolysis and 32,929 (55.4%) receiving primary percutaneous coronary intervention (PCI). The reperfusion rate varied across geographical regions (48.0%–73.5%). The overall rate increased from 60.0% to 69.7% from 2014 to 2019, mainly due to an increase in primary PCI within 12 h of symptom onset. Timely PCI, but not fibrinolysis alone, was associated with a decreased risk of in-hospital major adverse cardiovascular events compared with no reperfusion, with an adjusted hazard ratio (95% confidence interval) of 0.64 (0.54,0.76) for primary PCI at <12 h, 0.53 (0.37,0.74) for primary PCI at 12 to 24 h, 0.46 (0.25,0.82) for the pharmaco-invasive strategy, and 0.79 (0.54,1.15) for fibrinolysis alone. Conclusions: Nationwide quality improvement initiatives should be strengthened to increase the reperfusion rate and reduce inequality in China. Trial registration: www.ClinicalTrials. gov, NCT02306616