Miracle-dz/UniFAR-FTUs huggingface dataset:
The GDP-GTP Exchange Factor Collybistin: An Essential Determinant of Neuronal Gephyrin Clustering

Glycine receptors (GlyRs) and specific subtypes of GABAA receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain and three alternate C termini (CB1, CB2, and CB3). The presence of the SH3 domain negatively regulates the ability of collybistin to translocate gephyrin to submembrane microaggregates in transfected mammalian cells. Because the majority of native collybistin isoforms appear to harbor the SH3 domain, this suggests that collybistin activity may be regulated by protein-protein interactions at the SH3 domain. We localized the binding sites for collybistin and the GlyR β subunit to the C-terminal MoeA homology domain of gephyrin and show that multimerization of this domain is required for collybistin-gephyrin and GlyR-gephyrin interactions. We also demonstrate that gephyrin clustering in recombinant systems and cultured neurons requires both collybistin-gephyrin interactions and an intact collybistin pleckstrin homology domain. The vital importance of collybistin for inhibitory synaptogenesis is underlined by the discovery of a mutation (G55A) in exon 2 of the human collybistin gene (ARHGEF9) in a patient with clinical symptoms of both hyperekplexia and epilepsy. The clinical manifestation of this collybistin missense mutation may result, at least in part, from mislocalization of gephyrin and a major GABAA receptor subtype.
Optimization of the Removal Function of Dual-Rotation Magnetorheological Finishing

Magnetorheological finishing (MRF) is an advanced polishing technique capable of rapidly converging to the required surface figure. In this article, dual-rotation MRF tool is used to generate centrosymmetric removal function that can be W-shaped or Gaussian-shaped. Using W-shaped removal function and Gaussian-shaped removal function respectively, this article compares the convergence of surface error by simulating the finishing process through iterative calculations. Furthermore, it compares mid-spatial-frequency surface error caused by the two removal functions, through 2D FFT and PSD estimation in the frequency domain. From simulation, this article proves that Gaussian-shaped removal function is more advantageous than W-shaped removal function. To solve the problem that the removal function obtained in dual-rotation MRF is W-shaped, this paper presents the method of adjusting the distance between the axes of self-rotation and co-rotation to secure Gaussian-shaped removal function. Finally, experiments have been conducted to demonstrate validity of the method.
Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization.

The present study was undertaken in an attempt to recover new cytopathogenic agents from infants with severe lower respiratory illness (bronchopneumonia, bronchiolitis, and laryngotracheobronchitis) and to assess their etiologic significance in this disease complex. During the course of these investigations, two similar viruses were recovered -which were demonstrated to be indistinguishable from an agent associated with an outbreak of coryza in chimpanzees (CCA virus) studied by Morris, Blount and Savage (1). It is the purpose of this communication to describe the isolation, properties and characterization of these agents. In the second paper of this series (2) the epidemiologic aspects of human infection with these viruses will be discussed.
FRACTURE PROPERTIES OF GEOPOLYMER CONCRETE

This paper describes the results of an experimental investigation carried out to determine the fracture properties of geopolymer concrete. Geopolymer concrete of grade M30 was developed and the results were compared with that of conventional concrete of the same grade. A total number of 18 notched prisms of size 150mmx150mmx500mm with varying notch depth were prepared and subjected to bending test. Load deflection and load CMOD (crack mouth opening displacement) characteristics of each prism were observed. Based on this, the fracture parameters such as fracture energy and stress intensity factor were determined. The test results showed that geopolymer concrete exhibited enhanced performance compared to conventional concrete of the same grade. The results of experimental investigation have also been compared with those obtained by FEM analysis, and the comparison was found to be satisfactory.
New Connections: Cell-to-Cell HIV-1 Transmission, Resistance to Broadly Neutralizing Antibodies, and an Envelope Sorting Motif

ABSTRACT HIV-1 infection from cell-to-cell may provide an efficient mode of viral spread in vivo and could therefore present a significant challenge for preventative or therapeutic strategies based on broadly neutralizing antibodies. Indeed, Li et al. (H. Li, C. Zony, P. Chen, and B. K. Chen, J. Virol. 91:e02425-16, 2017, https://doi.org/10.1128/JVI.02425-16 ) showed that the potency and magnitude of multiple HIV-1 broadly neutralizing antibody classes are decreased during cell-to-cell infection in a context-dependent manner. A functional motif in gp41 appears to contribute to this differential susceptibility by modulating exposure of neutralization epitopes.
Image reconstruction for a general circle-plus trajectory

We develop an exact filtered backprojection (FBP) algorithm for a general class of circle-plus trajectories, which consist of two components: C and L. The first component C, which is analogous to a circle of the traditional circle-plus trajectories, is essentially any closed (e.g., not necessarily planar) continuous curve. The second component L is almost any continuous curve. The only condition is that L starts below C and ends above C. The distinctive feature of the new algorithm is that it does not depend on the global properties of L. When the source is located on L, the only thing one needs to know is how C projects onto the corresponding detector. The algorithm is especially convenient for the traditional circle-plus trajectories, which are implemented using a gantry and moving table. In this case we obtain a universal FBP algorithm, which is completely independent of how the table moves during the scan (provided that the main condition on L is satisfied). The results of testing the algorithm on two circle + helix trajectories demonstrate good image quality.
Automated Knowledge Engineering Tools in Planning: State-of-the-art and Future Challenges

Intelligent agents must have a model of the dynamics of the domain in which they act. Models can be encoded by human experts or, as required by autonomous systems, automatically acquired from observation. At the state of the art, there existseveral systems for automated acquisition of planning domain models. In this paper we present a brief overview of the automated tools that can be exploited to induce planning domain models. While reviewing the literature on the existing tools for Knowledge Engineering (KE), we do a comparative analysis of them. The analysis is based on a set of criteria. The aim of the analysis is to give insights into the strengths and weaknesses of the considered systems, and to provide input for new, forthcoming research on KE tools in order to address future challenges in the automated KE area.
Frequent PIK3CA Mutations in Colorectal and Endometrial Tumors With 2 or More Somatic Mutations in Mismatch Repair Genes.

BACKGROUND & AIMS Some colorectal and endometrial tumors with microsatellite instability not attributable to MLH1 hypermethylation or germline mutations contain 2 or more somatic mutations in genes encoding mismatch repair (MMR) proteins. We sought to define the molecular phenotype of this newly recognized tumor subtype. METHODS From 2 prospective studies of the efficacy of screening for Lynch syndrome, we identified patients with colorectal and endometrial tumors who had 2 or more somatic (but not germline) mutations in genes encoding MMR proteins (double somatic). We determined the frequencies of tumor mutations in PIK3CA, BRAF, KRAS, NRAS, and PTEN by targeted next-generation sequencing and used logistic-regression models to compare them with those from patients with Lynch syndrome, MLH1-hypermethylated, or microsatellite-stable tumors. We validated our findings using independent data sets from The Cancer Genome Atlas. RESULTS Among colorectal cancer cases, we found that 14 of 21 (67%) patients with double somatic tumors also had PIK3CA mutations, compared with 4 of 18 (22%) tumors from patients with Lynch syndrome, 2 of 10 (20%) tumors with MLH1 hypermethylation, and 12 of 78 (15%) tumors with microsatellite stability (P < .0001 for patients with double somatic tumors vs other subgroups). Mutations in PIK3CA were detected in all 13 patients with double somatic endometrial cancers (P = .04 compared with other subgroups). We did not detect BRAF mutations in patients with double somatic colorectal tumors or Lynch syndrome. We found highly similar results in a validation cohort from The Cancer Genome Atlas (113 patients with colorectal tumors, 178 endometrial tumors); 100% of double somatic cases had a somatic mutation in PIK3CA (P < .0001 compared with other subgroups). CONCLUSIONS Most patients with colorectal or endometrial tumors with 2 or more somatic (but not germline) mutations in MMR proteins also have mutations in PIK3CA; mutations in PIK3CA are detected at substantially higher frequencies in these double somatic tumors than in other microsatellite-instability subgroups. PIK3CA mutation status might be used to identify a specific group of colorectal tumors, and to select treatment or determine prognosis.
Development of norms for executive functions in typically-developing Indian urban preschool children and its association with nutritional status

ABSTRACT Executive functions (EFs) are essential and important for achieving success in children’s everyday lives and play a fundamental role in children’s cognitive, academic, social, emotional and behavioral functioning. A cross-sectional study was carried out to develop age- and sex-specific norms for EFs using the Behavior Rating Inventory of Executive Function – Preschool Version (BRIEF-P) among 2- to 5-year-olds from urban Bangalore, India. In addition, the association between EFs and anthropometric measures, a marker of nutritional status, is also examined. Primary caregivers of 412 children, equally distributed by age and sex, participated. Raw scores for each domain and indices were converted to standard t-scores and percentiles were computed. A t-score at or above 63 corresponding to the 90th percentile was considered as the cutoff for executive dysfunction in this sample. The prevalence of executive dysfunction is 10% based on the Global Executive Composite score of the BRIEF-P. The cutoff score for identifying executive dysfunction using existing United States (US) norms is higher compared to the cutoff score obtained in the current study. Therefore, using US norms for Indian children could result in the prevalence of executive dysfunction been underestimated. Multiple linear regression analysis revealed that stunted and underweight children have significantly elevated EF scores after adjusting for age, sex and socioeconomic status (SES; p < .01). A greater understanding of EFs in preschool children is important for the early identification of executive dysfunction and implementing interventions to improve their future prospects. This study also shows that undernourished children are more likely to have executive dysfunction.
Use of Stored Energy in PMSG Rotor Inertia for Low-Voltage Ride-Through in Back-to-Back NPC Converter-Based Wind Power Systems

The increasing installed wind power capacity has caused wind power generation to become a significant percentage of the entire electric power generation. As a consequence, the power system operators have included wind power plants regulation to improve the control of the overall power system, both in steady-state and transient operation. Therefore, wind power systems are required to verify the grid connection requirements stated by the power system operators. In presence of grid voltage dips, the low voltage ride-through (LVRT) requirement compliance produces a mismatch between the generated active power and the active power delivered to the grid. The conventional solution assumes that the active power surplus is dissipated in a dc-link resistor. In this paper, a control scheme for the back-to-back neutral-point-clamped converter is proposed. Under grid voltage dip, the controllers for generator-side and grid-side converters work concurrently to meet the LVRT requirement by storing the active power surplus in the turbine-generator mechanical system inertia while keeping constant the dc-link voltage. Simulation and experimental results verify the proposed control scheme.
Do Work-Related Mechanical and Psychosocial Factors Contribute to the Social Gradient in Low Back Pain?: A 3-Year Follow-Up Study of the General Working Population in Norway

Study Design. A prospective cohort study. Objective. The aim of the study was to investigate the extent to which work-related factors contribute to the social gradient in low back pain (LBP). Summary of Background Data. A social gradient in LBP is well established, but only a few studies have examined the extent to which exposure to mechanical and psychosocial work environment factors is a pathway for this gradient. Methods. A randomly drawn cohort from the general population in Norway aged 18 to 66 years was followed up for 3 years (n = 12,550, response rate at baseline = 67%). Eligible respondents were in paid work during 2006 and 2009 (n = 6819). Based on administrative register data respondents were coded into five educational levels (university/college ≥4 years was set as the reference group). Outcome of interest was self-reported moderate or severe LBP at follow-up adjusted for baseline LBP. Results. In total, 11.2% (397 individuals) men and 14.5% (461 individual) women reported LBP at follow-up. There was a strong social gradient ranging from 16.4% (elementary) to 6.4% (university/college ≥4 years). The corresponding figures among women were 22.4% and 7.5%. Corrected for age, LBP at baseline and working hours, educational level was a significant predictor of LBP at follow-up (odds ratios 1.8–2.3 in men and 1.7–3.1 in women). Adjusting for mechanical factors reduced the gradient by 39% to 43% in men and 28% to 34% in women. Adjusting for psychosocial factors reduced the gradient by 5% to 12% in men and increased the gradient by 7% to 11% in women. Conclusion. Work-related mechanical factors contributed substantially to the social gradient in LBP among both men and women. The impact of psychosocial factors was modest among men and contributed to an increased gradient among women. Level of Evidence: 3
Enhancing Osseointegration of Orthopaedic Implants with Titania Nanotube Surfaces

Category: Basic Sciences/Biologics Introduction/Purpose: Solid biologic fixation at the bone-implant interface provides long-term stability of orthopaedic implants. Historically, coatings and surface treatments on implant surfaces have been used to promote osseointegration of orthopaedic implants. The purpose of this research study is to evaluate two morphologies of titania nanotube (TiNT) surfaces via in vitro experiments as well as an in vivo model of femoral intramedullary fixation, in order to assess the influence of TiNT structure on de novo bone formation and bone-implant stability. Methods: TiNT structures were grown from Ti-6Al-4 V materials via an established electrochemical anodization process. Samples were either sonicated then annealed (Aligned TiNT) or annealed without prior sonication (Trabecular TiNT), to produce different morphologies. As-received titanium alloy was the control. Marrow-derived stem cells were isolated from long bones of Sprague Dawley rats and cultured on samples. Alkaline phosphatase (ALP) and osteocalcin (OC) expression by stem cells were assessed via ELISA. Cells were lysed and subjected to quantitative polymerase chain reaction (qPCR) to assess Col1a1, osteonectin, and IGF- 1 expression. An in vivo study evaluated bone formation at 4- and 12-week endpoints. Eight female Sprague Dawley rats per group per endpoint received bilateral Ti-6Al-4 V K-wires as femoral implants. Left femur received control, while right femur received Aligned/Trabecular TiNT K-wire. Bone formation was assessed via microCT, backscatter electron imaging (BEI), and non- decalcified histologic analyses. Results: Aligned and Trabecular TiNT groups demonstrated higher ALP activity than control at 2 and 3 weeks. The in vivo study demonstrated increased bone volume fractions (BV/TV) and total bone volume (TBV) for TiNT surfaces (microCT). The ratio of both BV/TV and TBV in the distal VOI were nearly equivalent for both TiNT surfaces, indicating similar bone formation between both TiNT surfaces and control. In the midshaft VOI, the ratios between TiNT surfaces and control were 1.5 or greater, indicating increased bone formation. At 12 weeks, the bone-implant contact fraction ratio (BEI) showed Aligned TiNT and Trabecular TiNT were 1.3 and 1.4 times greater than control, respectively. Histologic analysis showed both TiNT surfaces had 1.5 times the bone-implant contact as control. Conclusion: In vitro studies demonstrated improved support for osteogenic functions of cultured marrow-derived stem cells on TiNT surfaces compared to controls. μCT, BEI, and histologic analyses associated with the in vivo study demonstrated increased bone formation in the TiNT femora, at specific timepoints and VOIs.
Active Nuclear Import and Export Is Independent of Lumenal Ca2+ Stores in Intact Mammalian Cells

The nuclear pore complex (NPC) mediates communication between the cytoplasm and nucleus in eukaryotic cells. Active transport of large polypeptides as well as passive diffusion of smaller (≈10 kD) macromolecules through the NPC can be inhibited by depletion of intracellular Ca2+ stores. However, the physiological relevance of this process for the regulation of nucleocytoplasmic trafficking is not yet clear. We expressed green fluorescent protein (GFP)–tagged glucocorticoid receptor (GR) and mitogen-activated protein (MAP) kinase–activated protein kinase 2 (MK2) to study the effect of Ca2+ store depletion on active transport in HM1 cells, a human embryonic kidney cell line stably transfected with the muscarinic M1 receptor. Dexamethasone-induced nuclear import of GR-GFP and anisomycin-induced nuclear export of GFP-MK2 was monitored by confocal microscopy. We found that store depletion by carbachol, thapsigargin or ionomycin had no effect on GR-GFP import, whereas pretreatment with 1,2-bis-(o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid–acetoxymethyl ester (BAPTA-AM) attenuated import significantly. Export of GFP-MK2 was not influenced by any pretreatment. Moreover, carbachol stimulated GFP-MK2 translocation to the cytoplasm in the absence of anisomycin. These results demonstrate that Ca2+ store depletion in intact HM1 cells is not directly linked to the inhibition of active protein transport through the NPC. The inhibition of GR-GFP import but not GFP-MK2 export by BAPTA-AM presumably involves a depletion-independent mechanism that interferes with components of the nuclear import pathway.
Equilibrium in Queues Under Unknown Service Times and Service Value

In the operations research literature, the queue joining probability is monotonic decreasing in the queue length; the longer the queue, the fewer consumers join. Recent academic and empirical evidence indicates that queue-joining probabilities may not always be decreasing in the queue length. We provide a simple explanation for these nonmonotonic queue-joining strategies by relaxing the informational assumptions in Naor's model. Instead of imposing that the expected service time and service value are common knowledge, we assume that they are unknown to consumers, but positively correlated. Under such informational assumptions, the posterior expected waiting cost and service value increase in the observed queue length. As a consequence, we show that queue-joining equilibria may emerge for which the joining probability increases locally in the queue length. We refer to these as “sputtering equilibria. ” We discuss when and why such sputtering equilibria exist for discrete as well as continuously distributed priors on the expected service time with positively correlated service value.
Effect of DHA supplements during pregnancy on the concentration of PUFA in breast milk of Chinese lactating mothers

Abstract Objective: To determine whether there is an effect of prenatal supplementation with docosahexaenoic acid (DHA) on the concentration of polyunsaturated fatty acids (PUFAs) in the breast milk of Chinese lactating women. Methods: A total of 409 participants were recruited at the postpartum care center during their 1-month postpartum care. They were assigned to the supplement group or the control group according to whether or not DHA supplements were taken during pregnancy. Dietary intake was assessed with a food frequency questionnaire (FFQ). Breast milk samples were collected on 1 day between the 22nd and 25th day postpartum and levels of eight kinds of fatty acids in the breast milk were measured by gas chromatography. Results: DHA intake was divided into three levels (<57 mg/day, 57–185 mg/day and >185 mg/day). The concentration of DHA postpartum in the breast milk of the group receiving a DHA supplement >185 mg/day was significantly higher (P=0.003) compared to the control group. Conclusions: DHA intake >185 mg/day resulted in increased DHA concentrations in breast milk. This finding suggests that mothers with inadequate dietary intake of DHA should change their dietary habits to consume a diet rich in DHA or take sufficient DHA supplements to meet the average nutritional needs of infants.