Miracle-dz/UniFAR-FTUs huggingface dataset:
Surgical Technical Evidence Review for Elective Total Joint Replacement Conducted for the AHRQ Safety Program for Improving Surgical Care and Recovery

Background: Use of enhanced recovery pathways (ERPs) can improve patient outcomes, yet national implementation of these pathways remains low. The Agency for Healthcare Research and Quality (AHRQ; funder), the American College of Surgeons, and the Johns Hopkins Medicine Armstrong Institute for Patent Safety and Quality have developed the Safety Program for Improving Surgical Care and Recovery—a national effort to catalyze implementation of practices to improve perioperative care and enhance recovery of surgical patients. This review synthesizes evidence that can be used to develop a protocol for elective total knee arthroplasty (TKA) and total hip arthroplasty (THA). Study Design: This review focuses on potential components of the protocol relevant to surgeons; anesthesia components are reported separately. Components were identified through review of existing pathways and from consultation with technical experts. For each, a structured review of MEDLINE identified systematic reviews, randomized trials, and observational studies that reported on these components in patients undergoing elective TKA/THA. This primary evidence review was combined with existing clinical guidelines in a narrative format. Results: Sixteen components were reviewed. Of the 10 preoperative components, most were focused on risk factor assessment including anemia, diabetes mellitus, tobacco use, obesity, nutrition, immune-modulating therapy, and opiates. Preoperative education, venous thromboembolism (VTE) prophylaxis, and bathing/Staphylococcus aureus decolonization were also included. The routine use of drains was the only intraoperative component evaluated. The 5 postoperative components included early mobilization, continuous passive motion, extended duration VTE prophylaxis, early oral alimentation, and discharge planning. Conclusion: This review synthesizes the evidence supporting potential surgical components of an ERP for elective TKA/THA. The AHRQ Safety Program for Improving Surgical Care and Recovery aims to guide hospitals and surgeons in identifying the best practices to implement in the surgical care of TKA and THA patients.
Prevalence of sexually transmitted infections among young people in South Africa: A nested survey in a health and demographic surveillance site

Background Sexually transmitted infections (STIs) and bacterial vaginosis (BV) are associated with increased transmission of HIV, and poor reproductive and sexual health. The burden of STIs/BV among young people is unknown in many high HIV prevalence settings. We conducted an acceptability, feasibility, and prevalence study of home-based sampling for STIs/BV among young men and women aged 15–24 years old in a health and demographic surveillance site (HDSS) in rural KwaZulu-Natal, South Africa. Methods and findings A total of 1,342 young people, stratified by age (15–19 and 20–24 years) and sex were selected from the HDSS sampling frame; 1,171/1,342 (87%) individuals had ≥1 attempted home visit between 4 October 2016 and 31 January 2017, of whom 790 (67%) were successfully contacted. Among the 645 who were contacted and eligible, 447 (69%) enrolled. Consenting/assenting participants were interviewed, and blood, self-collected urine (men), and vaginal swabs (women) were tested for herpes simplex virus type 2 (HSV-2), chlamydia, gonorrhoea, syphilis, trichomoniasis, and BV. Both men and women reported that sample collection was easy. Participants disagreed that sampling was painful; more than half of the participants disagreed that they felt anxious or embarrassed. The weighted prevalence of STIs/BV among men and women, respectively, was 5.3% and 11.2% for chlamydia, 1.5% and 1.8% for gonorrhoea, 0% and 0.4% for active syphilis, 0.6% and 4.6% for trichomoniasis, 16.8% and 28.7% for HSV-2, and 42.1% for BV (women only). Of the women with ≥1 curable STI, 75% reported no symptoms. Factors associated with STIs/BV included having older age, being female, and not being in school or working. Among those who participated in the 2016 HIV serosurvey, the prevalence of HIV was 5.6% among men and 19% among women. Feasibility was impacted by the short study duration and the difficulty finding men at home. Conclusions A high prevalence of STIs/BV was found in this rural setting with high HIV prevalence in South Africa. Most STIs and HIV infections were asymptomatic and would not have been identified or treated under national syndromic management guidelines. A nested STI/BV survey within a HDSS proved acceptable and feasible. This is a proof of concept for population-based STI surveillance in low- and middle-income countries that could be utilised in the evaluation of STI/HIV prevention and control programmes.
Modeling the Distribution of Financial Returns by Functional Data Analysis

In this paper, we use functional data analysis to model a time varying unconditional distribution of financial intraday returns. This is in the spirit of the recent development of realized volatility modeling (e.g. Andersen et al, 2001), where one of the moments of this unconditional distribution, the realized volatility, is assumed to change smoothly over time. In the approach used in this paper we instead assume that the entire distribution function changes smoothly over time. This enables us to study auto- and cross dependencies of different parts of the unconditional distribution with no model assumptions but the smoothness of the distribution function. We develop a simulation based procedure for statistical inference of the model. Finally, we apply the method to the Swiss Franc-US Dollar exchange rate 1985-1991.
eview he role of aquaporin-4 in synaptic plasticity , memory and disease

Since the discovery of aquaporins, it has become clear that the various mammalian aquaporins play critical physiological roles in water and ion balance in multiple tissues. Aquaporin-4 (AQP4), the principal aquaporin expressed in the central nervous system (CNS, brain and spinal cord), has been shown to mediate CNS water homeostasis. In this review, we summarize new and exciting studies indicating that AQP4 also plays critical and unanticipated roles in synaptic plasticity and memory formation. Next, we
Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer

Lacking a satisfactory screening test, ovarian cancer is frequently diagnosed at a late stage, leading to poor patient outcomes. This study investigated the diagnostic value of circulating tumor cells (CTCs) in peripheral blood from patients with suspected ovarian tumors. Sixty-one women suspected of having an ovarian mass were prospectively enrolled in this study. CTCs were identified and counted using microfluidic isolation and immunofluorescent staining of CD45, HE4, and epithelial and mesenchymal (E&M) markers (epithelial cell adhesion molecule, cytokeratins, and vimentin). Thirty (49%) of the patients were diagnosed with ovarian cancer. DAPI+/E&M+/CD45-/HE4+ CTC counts were higher in these patients than in patients with benign tumors (p = 0.016). The receiver operating characteristic (ROC) curve showed that the sensitivity of CTCs was 73.3%, which was superior to that of CA125 (56.7%). In patients with elevated CA125 levels (≥35 U/ml), CTC counts still showed good specificity (86.7%). Our findings suggest the DAPI+/E&M+/CD45-/HE4+ CTC count is a useful diagnostic indicator in patients with suspected ovarian cancer.
Distributed Hypothesis Testing Over Discrete Memoryless Channels

A distributed binary hypothesis testing (HT) problem involving two parties, one referred to as the observer and the other as the detector is studied. The observer observes a discrete memoryless source (DMS) and communicates its observations to the detector over a discrete memoryless channel (DMC). The detector observes another DMS correlated with that at the observer, and performs a binary hypothesis test on the joint distribution of the two DMS’s using its own observed data and the information received from the observer. The trade-off between the type I error probability and the type II error-exponent of the HT is explored. Single-letter lower bounds on the optimal type II error-exponent are obtained by using two different coding schemes, a separate HT and channel coding scheme and a joint HT and channel coding scheme based on hybrid coding for the matched bandwidth case. Exact single-letter characterization of the same is established for the special case of testing against conditional independence, and it is shown to be achieved by the separate HT and channel coding scheme. An example is provided where the joint scheme achieves a strictly better performance than the separation based scheme.
Spatial Multiplexing for Non-Line-of-Sight Light-to-Camera Communications

Light-to-Camera Communications (LCC) have emerged as a new wireless communication technology with great potential to benefit a broad range of applications. However, the existing LCC systems either require cameras directly facing to the lights or can only communicate over a single link, resulting in low throughputs and being fragile to ambient illuminant interference. We present HYCACO, a novel LCC system, which enables multiple light emitting diodes (LEDs) with an unaltered camera to communicate via the non-line-of-sight (NLoS) links. Different from other NLoS LCC systems, the proposed scheme is resilient to the complex indoor luminous environment. HYCACO can decode the messages by exploring the mixed reflected optical signals transmitted from multiple LEDs. By further exploiting the rolling shutter mechanism, we present the optimal optical frequencies and camera exposure duration selection strategy to achieve the best performance. We built a hardware prototype to demonstrate the efficiency of the proposed scheme under different application scenarios. The experimental results show that the system throughput reaches 4.5 kbps on iPhone 6s with three transmitters. With the robustness, improved system throughput and ease of use, HYCACO has great potentials to be used in a wide range of applications such as advertising, tagging objects, and device certifications.
THE ROLE OF MED 12 IN TUMORIGENESIS

8 REVIEW OF THE LITERATURE . 10 1. Tumorigenesis . 10 2. Genetics of cancer . 11 2.1 Genomic alterations . 12 2.2 Epigenetic and other changes in tumorigenesis . 13 2.3 Cancer genes . 14 2.3.1 Oncogenes . 14 2.3.2 Tumor suppressor genes . 15 2.3.3 Stability genes . 16 2.4 Inherited susceptibility for cancer . 16 2.5 Genome-wide methods in cancer genetics . 17 3. Uterine leiomyomas . 18 3.1 Development of uterine leiomyomas . 19 3.2 Chromosomal alterations in uterine leiomyomas . 20 3.3 Hereditary leiomyomatosis and renal cell cancer syndrome . 21 3.4 MED12 mutations in uterine leiomyomas . 22 4. MED12 . 23 4.1 MED12 as a subunit of the Mediator complex . 25 4.2 MED12 in signaling pathways . 27 4.3 MED12 mutations in the germ line . 28 4.4 Somatic MED12 mutations in other tumor types? 29 AIMS OF THE STUDY . 31 MATERIALS AND METHODS . 32 1. Study subjects and samples . 32 1.1 Tumor samples (I-V) . 32 1.2 Normal tissue samples (I-III) . 35 1.3 Cell lines (II, V) . 35 2. Methods . 36 2.1 Histopathological evaluation (I, III) . 36 2.2 DNA and RNA extraction (I-V) . 36 2.3 Sanger sequencing (I-V) . 37
User Perspectives on Exergames Designed to Explore the Hemineglected Space for Stroke Patients With Visuospatial Neglect: Usability Study

Background Visuospatial neglect due to stroke is characterized by the inability to perceive stimuli emerging in the area opposite to the side of brain damage. Besides adopting conventional rehabilitation methods to treat neglect symptoms, the use of virtual reality (VR) is becoming increasingly popular. We designed a series of 9 exergames aimed to improve exploration of the neglected side of space. When new VR interventions are designed, it is important to assess the usability aspects of such management strategies within the target population. To date, most studies used questionnaires to assess user satisfaction with the intervention or product being tested. However, only a combination of both quantitative and qualitative data allows a full picture of user perspective. Objective The purpose of this study was to quantitatively and qualitatively assess patient and therapist perspectives of a VR intervention based on the series of 9 exergames designed to explore hemineglected space. Specifically, we wanted to evaluate (1) perceived-user friendliness of the exergames, (2) attitude towards using the exergames, and (3) intention to use the exergames in the future. Methods A total of 19 participants (7 patients, 12 therapists) evaluated the exergames they had used 5 times a week during 3 weeks. The Technology Acceptance Model (TAM) questionnaire was filled out after the intervention. Based on those responses, we conducted focus group interviews (with therapists) and individual interviews (with patients). To analyze the TAM questionnaires, we used descriptive statistics. We adopted content and comparative analysis to analyze the interviews and drew illustration maps to analyze the focus group interviews. Results The therapists took a more critical stance with a mean TAM questionnaire total score of 48.6 (SD 4.5) compared to the patients who had a mean total score of 56.1 (SD 12.3). The perceived user-friendliness score was 5.6 (SD 1.4) for patients and 4.9 (SD 1.4) for therapists. The attitude towards using the exergames was rated 4.8 (SD 1.9) by patients and 3.6 (SD 1.4) by therapists, respectively. The intention to use the exergames in the future was rated 3.9 (SD 2.1) by patients and 3.7 (SD 1.8) by therapists. We gained information on how to improve the exergames in the interviews. Conclusions Patients and therapists perceived the exergames as user-friendly; however, using the games further with the actual test version was not perceived as conceivable. The therapists were generally more critical towards future use than the patients. Therefore, involving both users to achieve acceptable and user-friendly versions of game-based rehabilitation for the future is deemed crucial and warranted. Trial Registration Clinicaltrials.gov NCT02353962; https://clinicaltrials.gov/ct2/show/NCT02353962 (Archived by WebCite at http://www.webcitation.org/6soxIJlAZ)
Branch history table indexing to prevent pipeline bubbles in wide-issue superscalar processors

Presents a comparison of superscalar and decoupled access/execute architectures. Both architectures attempt to exploit instruction-level parallelism by issuing multiple instructions per cycle, employing dynamic scheduling to maximize performance. Simulation results are presented for four different configurations, demonstrating that the architectural queues of the decoupled machines provide similar functionality to register renaming, dynamic loop unrolling, and out-of-order execution of the superscalar machines with significantly less complexity. <<ETX>>
Using Meta-analytic Structural Equation Modeling to Study Developmental Change in Relations Between Language and Literacy.

The purpose of this review was to introduce readers of Child Development to the meta-analytic structural equation modeling (MASEM) technique. Provided are a background to the MASEM approach, a discussion of its utility in the study of child development, and an application of this technique in the study of reading comprehension (RC) development. MASEM uses a two-stage approach: first, it provides a composite correlation matrix across included variables, and second, it fits hypothesized a priori models. The provided MASEM application used a large sample (N = 1,205,581) of students (ages 3.5-46.225) from 155 studies to investigate the factor structure and relations among components of RC. The practical implications of using this technique to study development are discussed.
Genomic profiling is predictive of response to cisplatin treatment but not to PI3K inhibition in bladder cancer patient-derived xenografts

Purpose Effective systemic therapeutic options are limited for bladder cancer. In this preclinical study we tested whether bladder cancer gene alterations may be predictive of treatment response. Experimental design We performed genomic profiling of two bladder cancer patient derived tumor xenografts (PDX). We optimized the exome sequence analysis method to overcome the mouse genome interference. Results We identified a number of somatic mutations, mostly shared by the primary tumors and PDX. In particular, BLCAb001, which is less responsive to cisplatin than BLCAb002, carried non-sense mutations in several genes associated with cisplatin resistance, including MLH1, BRCA2, and CASP8. Furthermore, RNA-Seq analysis revealed the overexpression of cisplatin resistance associated genes such as SLC7A11, TLE4, and IL1A in BLCAb001. Two different PIK3CA mutations, E542K and E545K, were identified in BLCAb001 and BLCAb002, respectively. Thus, we tested whether the genomic profiling was predictive of response to a dual PI3K/mTOR targeting agent, LY3023414. Despite harboring similar PIK3CA mutations, BLCAb001 and BLCAb002 exhibited differential response, both in vitro and in vivo. Sustained target modulation was observed in the sensitive model BLCAb002 but not in BLCAb001, as well as decreased autophagy. Interestingly, computational modelling of mutant structures and affinity binding to PI3K revealed that E542K mutation was associated with weaker drug binding than E545K. Conclusions Our results suggest that the presence of activating PIK3CA mutations may not necessarily predict in vivo treatment response to PI3K targeted therapies, while specific gene alterations may be predictive for cisplatin response in bladder cancer models and, potentially, in patients as well.
On the enzymatic synthesis of unsaturated fatty acids in Escherichia coli.

The fatty acid synthetase system from E. coli catalyzes the formation of both saturated and unsaturated fatty acids from acetyl-CoA,I malonyl-CoA, and TPNH, in the presence of acyl carrier protein (ACP). 2-4 Olefinic acids are favored; cis-vaccenic acid is the major product, comprising 70-80 per cent of the nonpolar fatty acids formed. cis-vaccenate.
An initial study of a lightweight process for change identification and regression test selection when source code is not available

Various regression test selection techniques have been developed and have shown to improve testing cost effectiveness via improving efficiency. The majority of these test selection techniques rely on access to source code for change identification. However, when new releases of COTS components are made available for integration and testing, source code is often not available to guide in regression test selection. In this paper we describe a lightweight integrated-black-box approach for component change identification (I-BACCI) process for selection of regression tests for user/glue code that uses COTS components. I-BACCI is applicable when component licensing agreements do not preclude binary code analysis. A case study of the process was conducted on an ABB product that uses a medium-scale internal ABB software component. Six releases of the component were examined to evaluate the efficacy of the proposed process. The result of the case study indicates that this process can reduce the required regression tests by 40% on average
A single β-octyl glucoside molecule induces HIV-1 Nef dimer formation in the absence of partner protein binding

The HIV-1 Nef accessory protein is essential for viral pathogenicity and AIDS progression. Nef forms complexes with multiple host cell factors to facilitate viral replication and promote immune escape of HIV-infected cells. Previous X-ray crystal structures demonstrate that Nef forms homodimers, the orientation of which are influenced by host cell binding partners. In cell-based fluorescence complementation assays, Nef forms homodimers at the plasma membrane. However, recombinant Nef proteins often exist as monomers in solution, suggesting that membrane interaction may also trigger monomer to dimer transitions. In this study, we show that monomeric Nef core proteins can be induced to form dimers in the presence of low concentrations of the non-ionic surfactant, β-octyl glucoside (βOG). X-ray crystallography revealed that a single βOG molecule is present in the Nef dimer, with the 8-carbon acyl chain of the ligand binding to a hydrophobic pocket formed by the dimer interface. This Nef-βOG dimer interface involves helix αB, as observed in previous dimer structures, as well as a helix formed by N-terminal residues 54–66. Nef dimer formation is stabilized in solution by the addition of βOG, providing biochemical validation for the crystal structure. These observations together suggest that the interaction with host cell lipid mediators or other hydrophobic ligands may play a role in Nef dimerization, which has been previously linked to multiple Nef functions including host cell protein kinase activation, CD4 downregulation, and enhancement of HIV-1 replication.
Common lizards break Dollo’s law of irreversibility: genome-wide phylogenomics support a single origin of viviparity and re-evolution of oviparity

Dollo’s law of irreversibility states that once a complex trait has been lost in evolution, it cannot be regained. It is thought that complex epistatic interactions and developmental constraints impede the re-emergence of such a trait. Oviparous reproduction (egg-laying) requires the formation of an eggshell and represents an example of such a complex trait. In reptiles, viviparity (live-bearing) has evolved repeatedly but it is highly disputed if oviparity has re-evolved. Here, using up to 194,358 SNP loci and 1,334,760 bp of sequence, we reconstruct the phylogeny of viviparous and oviparous lineages of common lizards and infer the evolutionary history of parity modes. Our phylogeny strongly supports six main common lizard lineages that have been previously identified. We find very high statistical support for a topological arrangement that suggests a reversal to oviparity from viviparity. Our topology is consistent with highly differentiated chromosomal configurations between lineages, but disagrees with previous phylogenetic studies in some nodes. While we find high support for a reversal to oviparity, more genomic and developmental data are needed to robustly test this and assess the mechanism by which a reversal might have occurred.
Skin-interfaced systems for sweat collection and analytics

The advances in sweat collection and analytics follow from a convergence of electronics, electrochemistry, and microfluidics. Recent interdisciplinary advances in materials, mechanics, and microsystem designs for biocompatible electronics, soft microfluidics, and electrochemical biosensors establish the foundations for emerging classes of thin, skin-interfaced platforms capable of capturing, storing, and performing quantitative, spatiotemporal measurements of sweat chemistry, instantaneous local sweat rate, and total sweat loss. This review summarizes scientific and technical progress in this area and highlights the implications in real time and ambulatory modes of deployment during physical activities across a broad range of contexts in clinical health, physiology research, fitness/wellness, and athletic performance.