Miracle-dz/UniFAR-FTUs huggingface dataset:
Retrograde transport of intact poliovirus through the axon via the fast transport system.

Intramuscularly inoculated poliovirus is thought to spread to the central nervous system through neural pathways in humans, monkeys, and the transgenic (Tg) mice carrying the human poliovirus receptor (PVR) gene. To gain insight into molecular mechanisms for the retrograde axonal transport of poliovirus, resulting in the expression of neurovirulence, a poliovirus-sensitive ICR-PVRTg21 mouse line (Tg21) was used as an animal model for poliomyelitis. We detected poliovirus antigens in axons of the sciatic nerve. All of the Tg21 mice, which had been inoculated into the calves with 1 x 10(6) pfu of the Mahoney strain of type 1 poliovirus, showed symptoms of paralysis in the inoculated limbs (initial paralysis) within 48 h after the inoculation. The appearance of this initial paralysis was observed in mice whose sciatic nerves were transected at various times after virus inoculation. The results were indicators of the velocity of poliovirus transportation through the sciatic nerves under analysis. Poliovirus-related materials recovered from the sciatic nerve were mainly composed of intact 160S virion particles. The amount of 160S particle recovered was greatly reduced by coinjection with anti-PVR monoclonal antibody. These results suggest that one of the fast retrograde axonal transport systems is involved in poliovirus dissemination through the sciatic nerve and that IM-inoculated poliovirus is incorporated into the sciatic nerve as intact particles in a PVR-dependent manner, as it is in humans.
Multiple Regions of ETO Cooperate in Transcriptional Repression*

In acute myeloid leukemias (AMLs) with t(8;21), the transcription factor AML1 is juxtaposed to the zinc finger nuclear protein ETO (Eight-Twenty-One), resulting in transcriptional repression of AML1 target genes. ETO has been shown to interact with corepressors, such as N-CoR and mSin3A to form complexes containing histone deacetylases. To define regions of ETO required for maximal repressor activity, we analyzed amino-terminal deletions in a transcriptional repression assay. We found that ETO mutants lacking the first 236 amino acids were not affected in their repressor activity, whereas a further deletion of 85 amino acids drastically reduced repressor function and high molecular weight complex formation. This latter mutant can still homodimerize and bind to N-CoR but shows only weak binding to mSin3A. Furthermore, we could show that a “core repressor domain” comprisingnervy homology region 2 and its amino- and carboxyl-terminal flanking sequences recruits mSin3A and induces transcriptional repression. These results suggest that mSin3A and N-CoR bind to ETO independently and that both binding sites cooperate to maximize ETO-mediated transcriptional repression. Thus, ETO has a modular structure, and the interaction between the individual elements is essential for the formation of a stable repressor complex and efficient transcriptional repression.
Technician losses in public residential facilities.

A cross-organizational longitudinal survey was undertaken of state-operated residential facilities to gauge the extent to which technician-level work forces are affected by turnover and absenteeism. Twelve facilities participated in this study for a period of 9 months. Monthly turnover rates ranged from . 6 to 4.9 percent, with a average of 2.4 percent. Turnover rates appeared to be influenced by the size, location, and staffing patterns of the individual institution. With respect to absenteeism, the average "lost time rate" was 6.7 percent. Various standards suggest that absenteeism exceeding 4.5 percent disrupts recommended staffing ratios. One may conclude that employee withdrawal, whether in the form of avoidable turnover or unscheduled absenteeism, represents a serious managerial problem for many institutions.
Endocannabinoid mechanisms of pain modulation

Cannabinoids are antinociceptive in animal models of acute, tissue injury-, and nerve injury-induced nociception. This review examines the biology of endogenous cannabinoids (endocannabinoids) and behavioral, neurophysiological, and neuroanatomical evidence supporting the notion that cannabioids play a role in pain modulation. Behavioral pharmacological approaches, in conjunction with the identification and quantification of endocannabinoids through the use of liquid and gas chromatography mass spectrometry, have providedinsight into the functional roles of endocannabinoids in pain modulation. Here we examine the distribution of cannabinoid receptors and endocannabinoid-hydrolyzing enzymes within pain modulatory circuits together with behavioral, neurochemical, and neurophysiological studies that suggest a role for endocannabinoid signaling in pain modulation. This review will provide a comprehensive evaluation of the roles of the endocannabinoids 2-arachidonoyl-glycerol and anandamide in stress-induced analgesia. These findings provide a functional framework with which to understand the roles of endocannabinoids in nociceptive processing at the supraspinal level.
SOX2 Is an Oncogene Activated by Recurrent 3q26.3 Amplifications in Human Lung Squamous Cell Carcinomas

Squamous cell carcinoma (SCC) of the lung is a frequent and aggressive cancer type. Gene amplifications, a known activating mechanism of oncogenes, target the 3q26-qter region as one of the most frequently gained/amplified genomic sites in SCC of various types. Here, we used array comparative genomic hybridization to delineate the consensus region of 3q26.3 amplifications in lung SCC. Recurrent amplifications occur in 20% of lung SCC (136 tumors in total) and map to a core region of 2 Mb (Megabases) that encompasses SOX2, a transcription factor gene. Intense SOX2 immunostaining is frequent in nuclei of lung SCC, indicating potential active transcriptional regulation by SOX2. Analyses of the transcriptome of lung SCC, SOX2-overexpressing lung epithelial cells and embryonic stem cells (ESCs) reveal that SOX2 contributes to activate ESC-like phenotypes and provide clues pertaining to the deregulated genes involved in the malignant phenotype. In cell culture experiments, overexpression of SOX2 stimulates cellular migration and anchorage-independent growth while SOX2 knockdown impairs cell growth. Finally, SOX2 over-expression in non-tumorigenic human lung bronchial epithelial cells is tumorigenic in immunocompromised mice. These results indicate that the SOX2 transcription factor, a major regulator of stem cell function, is also an oncogene and a driver gene for the recurrent 3q26.33 amplifications in lung SCC.
Functional Variants of Fc Gamma Receptor (FCGR2A) and FCGR3A Are Not Associated with Susceptibility to Systemic Sclerosis in a Large European Study (EUSTAR)

Objective. To investigate the possible role of FCGR2A 519A>G and FCGR3A 559A>C functional polymorphisms in the genetic predisposition to susceptibility to systemic sclerosis (SSc) or clinical phenotype. Methods. A total of 1566 patients with SSc and 2271 geographically matched controls were included in our study. We analyzed the genotype and allele frequencies of the FCGR2A 519A>G and FCGR3A 559A>C functional variants in 6 independent European cohorts of white patients with SSc, and white controls. The cohorts comprised 165 Dutch patients with SSc and 1326 controls, 236 Spanish patients with SSc and 257 controls, 267 German patients with SSc and 270 controls, 202 Swedish patients with SSc and 261 controls, 416 Italian patients with SSc and 157 controls, and additionally 280 English patients with SSc. Genotyping was performed using Taqman 5′ allelic discrimination assay. The study reached a 99% power to detect the effect of a polymorphism at an OR of 1.3. Results. Neither FCGR2A 519A>G nor FCGR3A 559A>C was significantly associated with susceptibility to SSc. We did not find an association with specific disease phenotypes, limited or diffuse cutaneous involvement, autoantibody profiles, or pulmonary involvement. Conclusion. Our study strongly suggests the lack of a role for the FCGR2A 519A>G and FCGR3A 559A>C polymorphisms in SSc susceptibility or clinical phenotype in 6 independent European cohorts.
Reasoning from uncertain premises: Effects of expertise and conversational context

Four experiments investigated uncertainty about a premise in a deductive argument as a function of the expertise of the speaker and of the conversational context. The procedure mimicked everyday reasoning in that participants were not told that the premises were to be treated as certain. The results showed that the perceived likelihood of a conclusion was greater when the major or the minor premise was uttered by an expert rather than a novice (Experiment 1). The results also showed that uncertainty about the conclusion was higher when the major premise was uttered by a novice and an alternative premise by an expert, compared to when the major premise was uttered by an expert and the alternative by a novice (Experiment 2). Similarly, the believability of a conclusion was considerably lower when the minor premise was uttered by a novice and denied by an expert, as opposed to when an expert uttered the minor premise and a novice denied it (Experiment 3). Experiment 4 showed that the nature of the uncertainty induced by a denial of the minor premise depended on whether or not the context was a conversation. These results pose difficult problems for current theories of reasoning, as current theories are based on the results of experiments in which the premises are treated as certain. Our discussion of the results emphasises the importance of pragmatics in reasoning, namely, the role of general knowledge about the world in assessing the probability of a premise uttered by an expert or a novice and the role of interpretations of the premise based on pragmatic inferences in revising these initial probabilities.
Pediatric Intussusception in Northern Iran: Comparison of Recurrent With Non-Recurrent Cases

Background: Intussusception represents as the invagination of a part of the intestine into itself and is the most common cause of intestinal obstruction in infants and children between 6 months to 3-years-old. Objectives: The objective of this study was to determine the recurrence rate and predisposing factors of recurrent intussusception. Patients and Methods: The medical records of children aged less than 13-years-old with confirmed intussusception who underwent reduction at a tertiary academic care in northern Iran (Mazandran), from 2001 to 2013 were reviewed. Data were extracted and recurrence rate was determined. The two groups were compared by chi square, Fisher, Mann-Whitney and t-test. Diagnosed cases of intussusception consisted of 237 children. Results: Average age of the patients was 19.57 ± 19.43 months with a peak of 3 to 30 months. Male to female ratio was 1.65 and this increased by aging. Recurrence rate was 16% (38 cases). 87 (36.7%) underwent surgery. These were mainly children under one year old. In 71% (40) of episodes recurrence occurred 1 to 7 times within 6 months. The recurrence occurred in 29 (23.5%) children in whom a first reduction was achieved with barium enema (BE) and 5 (5.7%) children who had an operative reduction (P < 0.001) in the first episode. Pathological leading points (PLPs) were observed in 5 cases; 2.6% in recurrence group versus 2% in non-recurrence group (P = 0.91). Three patients had intestinal polyp, 2 patient’s lymphoma and Mackle’s diverticulum. Age (P = 0.77) and sex (P = 0.38) showed no difference between the two groups. PLPs were observed in 1.4% of children aged 3 months to 5 years. This was 13.3%, in older children (P = 0.02). Conclusions: The recurrence of intussusception was related to the method of treatment in the first episode and it was 5-fold higher in children with BE than in operative reduction. Recurrent intussusceptions were not associated with PLPs, they were more idiopathic.
The Relationship Between Cell Phone Use and Academic Performance in a Sample of U.S. College Students

The cell phone is ever-present on college campuses and is frequently used in settings where learning occurs. This study assessed the relationship between cell phone use and actual college grade point average (GPA) after controlling for known predictors. As such, 536 undergraduate students from 82 self-reported majors at a large, public university were sampled. A hierarchical regression (R2 = .449) demonstrated that cell phone use was significantly (p < .001) and negatively (β = −.164) related to actual college GPA after controlling for demographic variables, self-efficacy for self-regulated learning, self-efficacy for academic achievement, and actual high school GPA, which were all significant predictors (p < .05). Thus, after controlling for other established predictors, increased cell phone use was associated with decreased academic performance. Although more research is needed to identify the underlying mechanisms, findings suggest a need to sensitize students and educators about the potential academic risks associated with high-frequency cell phone use.
A CHANDRA SURVEY AND AN EXTENSION TO QUASISOFT SOURCES

We report on very soft X-ray sources (VSSs) in M31. In a survey which was most sensitive to soft sources in four 8 ×8 regions covered byChandra’s ACIS-S S3 CCD, we find 33 VSSs that appear to belong to M31. Fifteen VSSs have spectral characteristics mirroring the s upersoft X-ray sources studied in the Magellanic Cloud and Milky Way (kTe f f ≤100 eV); we therefore call these “classical” supersoft sour ces, or simply supersoft sources (SSSs). Eighteen VSSs may either have small ( < 10%) hard components, or slightly higher effective tempera tures (but still< 350 eV). We refer to these VSSs as quasisoft sources (QSSs). W hile hot white dwarf models may apply to SSSs, the effective temperatures of QSSs are too high, unl ess, e.g., the radiation emanates from only a small portion of surface. Two of the SSSs were first detected and ide ntified as such through ROSATobservations. One SSS and one QSS may be identified with symbiotics, and 2 SSSs wi th upernova remnants. Both SSSs and QSSs in the disk are found near star-forming regions, possibly in dicating that they are young. VSSs in the outer disk and halo are likely to be old systems; in these regions, there are more QSSs than SSSs, which is opposite to what is found in fields closer to the galaxy center. The largest den sity of bright VSSs is in the bulge; some of the bulge sources are close enough to the nucleus to be remnants of the t idal disruption of a giant by the massive central black hole. By usingChandradata in combination withROSATandXMM observations, we find most VSSs to be highly variable, fading from or brightening toward detecta bility on time scales of months. There is evidence for VSSs with low luminosities ( ∼ 1036 erg s−1). Subject headings: galaxies: individual (M31) — X-rays: binaries — X-rays: gal axy
Daily Exercise Reduces Measures of Heart Rate and Blood Pressure Variability in Hypertensive Rats

This study was designed to test the hypothesis that daily spontaneous running (DSR) reduces measures of heart rate and blood pressure variability in spontaneously hypertensive rats (SHR). After 8 weeks of DSR or sedentary control, rats were chronically instrumented with arterial catheters. Daily exercise reduced most measures of heart rate (HR) and blood pressure variability. Specifically DSR decreased heart rate, Low Frequency Power (LF: 0.19–0.61 Hz), and Low Frequency/High Frequency (HF: 1.2–2.5 Hz) ratio of HR. Furthermore, Total Power (TP), LF power, and LF/HF ratio of systolic blood pressure were reduced by daily spontaneous running. Finally, TP, LF and HF powers and LF/HF ratios of diastolic blood pressure were reduced by daily spontaneous running. These data demonstrate that daily exercise reduces sympathetic activity and possibly increases cardiac reserve in hypertensive animals.
Variable approaches to genetic counseling for microarray regions of homozygosity associated with parental relatedness

SNP microarrays are capable of detecting regions of homozygosity (ROH) which can suggest parental relatedness. This study was designed to describe pre‐ and post‐test counseling practices of genetics professionals regarding ROH, explore perceived comfort and ethical concerns in the follow‐up of such results, demonstrate awareness of laws surrounding duty to report consanguinity and incest, and allow respondents to share their personal experiences with results suggesting a parental relationship. A 35 question survey was administered to 240 genetic counselors and geneticists who had ordered or counseled for SNP microarray. The results are presented using descriptive statistics. There was variation in both pre‐ and post‐test counseling practices of genetics professionals. Twenty‐five percent of respondents reported pre‐test counseling that ROH can indicate parental relatedness. The most commonly reported ethical concern was disclosure of findings suggesting parental relatedness to parents of the patient; only 48.4% reported disclosing parental relatedness when indicated. Fifty‐seven percent felt comfortable receiving results suggesting parental consanguinity while 17% felt comfortable receiving results suggesting parental incest. Twenty percent of respondents were extremely/moderately familiar with the laws about duty to report incest. Personal experiences in post‐test counseling included both parental acknowledgement and denial of relatedness. This study highlights the differences in genetics professionals' pre‐ and post‐test counseling practices, comfort, and experiences surrounding parental relatedness suggested by SNP microarray results. It identifies a need for professional organizations to offer guidance to genetics professionals about how to respond to and counsel for molecular results suggesting parental consanguinity or incest. © 2013 Wiley Periodicals, Inc.
Competitiveness, productivity, competition and structural reforms: the Belgian case

La competitivite belge au regard de celle des pays voisins a ete deterioree par une croissance des salaires horaires insuffisamment compensee par la croissance de la productivite. La croissance de la Productivite totale des facteurs (PTF) a ete particulierement moins soutenue que dans les pays voisins. Parmi les determinants de la PTF, les conditions de concurrence peuvent avoir joue un role important et sont un element interessant pour la politique economique dans la mesure ou l’amelioration de ces conditions n’est pas budgetairement couteuse. Cet article explore la mesure des conditions de concurrence en analysant cinq methodes utilisees dans la litterature economique. Ces methodes sont les marges prix-cout, les entrees-sorties, la concentration du marche, la stabilite du marche et la sensibilite des benefices. Chaque methode presente des limites, a la fois d’un point de vue theorique et empirique mais peut etre utilisee comme une approximation de l’intensite et de l’evolution de la concurrence sous-jacente. Le resultat montre que la Belgique performe moins bien (selon trois methodes) ou, au mieux, aussi bien (selon deux methodes) que la moyenne des pays membres de l’Union europeenne, mais certainement jamais mieux. L’intensite de la concurrence pourrait cependant s’etre amelioree quelque peu. Meme si l’article n’etablit pas statistiquement de relation entre concurrence et PTF, les resultats suggerent que la faible performance en termes de PTF pourrait etre due a des conditions de concurrence defavorables.Codes JEL : D24, L11
Cells of origin of the spinohypothalamic tract in the rat

We recently demonstrated that large numbers of neurons in the spinal cord of rats project directly to the hypothalamus. In the present study, we used the retrograde tracer Fluoro‐Gold (FG) to examine this projection more completely. In the first series ofss studies, we attempted to label the entire population of spinal cord neurons that project to the hypothalamus. Injections that virtually filled the hypothalamus on one side without spreading into any other diencephalic area labeled a large number of neurons (estimated to be more than 9,000 in the case with the most neurons labeled) bilaterally at all levels of the spinal cord. Approximately 60° of the labeled neurons were contralateral to the injection. The greatest number of labeled neurons was found within the deep dorsal horn. Many were also found within the lateral spinal nucleus, the superficial dorsal horn, and the gray matter surrounding the central canal. A small number of labeled cells was located in the intermediate zone and ventral horn of the spinal gray matter. Labeled neurons were distributed bilaterally within the sacral parasympathetic nucleus and trigeminal nucleus caudalis.
Kamikihito ameliorates tumor‐induced sickness behavior in mice

Kamikihito (KKT), a Kampo medicine composed of 14 herbs, has been used clinically to treat psychiatric disorders such as anxiety, insomnia, amnesia and depression. Sickness behavior refers to a series of behavioral and psychological changes that occur due to cancer and inflammatory disease. The etiological mechanism is not known in detail, and thus there is no established standard therapy for sickness behavior. In this study, we examined the effects of KKT on tumor‐induced sickness behavior in mice, and compared the effects of KKT with those of milnacipran, a serotonin noradrenaline re‐uptake inhibitor.
If I Help My Partner, Will It Hurt Me? Perceptions of Sacrifice in Romantic Relationships

Abstract We proposed and tested a theory of the associations among relationship commitment, perceived harmfulness of relationship sacrifices to the self, and relationship and individual well–being. Results from a cross–sectional study of 145 couples provided reliability and validity data for a measure of the extent to which relationship sacrifices are perceived to be harmful to the self. As predicted, greater perceived sacrifice harmfulness was associated with lower relationship commitment, poorer couple functioning, and higher depressive symptomatology. However, commitment was more robustly predictive of perceived sacrifice harmfulness for males than females; in women, the association was weaker and could be accounted for by partner commitment and relationship satisfaction. Perceived sacrifice harmfulness partially mediated the association between commitment and relationship functioning in males.
Evaluation of automatic shot boundary detection on a large video test suite

The challenge facing the indexing of digital video information in order to support browsing and retrieval by users, is to design systems that can accurately and automatically process large amounts of heterogeneous video. The segmentation of video material into shots and scenes is the basic operation in the analysis of video content. This paper presents a detailed evaluation of a histogram-based shot cut detector based on eight hours of TV broadcast video. Our observations are that the selection of similarity thresholds for determining shot boundaries in such broadcast video is difficult and necessitates the development of systems that employ adaptive thresholding in order to address the huge variation of characteristics prevalent in TV broadcast video.