Miracle-dz/UniFAR-FTUs huggingface dataset:
PDIP38 is a novel mitotic spindle-associated protein that affects spindle organization and chromosome segregation

In order to maintain genomic integrity during mitosis, cells assemble the mitotic spindle to separate sister chromosomes to the two daughter cells. A variety of motor- and non motor-proteins are involved in the organization and regulation of this complex apparatus. DNA polymerase δ-interacting protein 38 (PDIP38) is a highly conserved protein and has so far been shown to be a cytoplasmic and nuclear protein. Cell cycle dependent nuclear localization and the interaction with DNA polymerase δ and proliferating cell nuclear antigen (PCNA) indicate a role for PDIP38 in DNA modification and/or proliferation. Here, we show for the first time that PDIP38 localizes to the mitotic spindle throughout mitosis. Using anti-PDIP38 antibody injections and siRNA silencing, we demonstrate that PDIP38 loss-of-function causes problems with spindle organization, aberrant chromosome segregation, and multinucleated cells. Taken together, the data indicate different roles for PDIP38 in safeguarding a proper cell division at various stages of the cell cycle, including DNA synthesis and repair, organization of the mitotic spindle and chromosome segregation.
Ad Hoc Networking Pattern Language

Ad hoc networking is based on the principle of spontaneous addition, discovery, and usage of services in a network. Various technologies such as JiniTM [Sun04], UPnP [UPnP04], and . NET are built around the concept of ad hoc networking. While each technology is either platform or language dependent, they share a common underlying architecture which can be expressed using a pattern language. The pattern language abstracts away from any specific language, platform, or technology. We present this pattern language here with primary focus on resource management patterns.
On an Application of Dynamic Programming to the Synthesis of Logical Systems

In this paper we wish to initiate the study of the application of dynamic programming to the domain of problems arising in the synthesis of logical systems. In a number of fields one encounters the problem of converting a system in one state into another state in a most efficient fashion—in mathematical economics, in the theory of control processes, in network theory, and in trajectory processes. Here we wish to consider a type of question which arises in the design of computers and switching circuits. We shall first treat the problem in general terms, and then consider a special example to illustrate the methods.
Transition over 35 years in the incidence rates of primary central nervous system tumors in Shanghai, China and histological subtyping based on a single center experience spanning 60 years.

BACKGROUND Only few epidemiological data on primary central nervous system (CNS) tumors in Shanghai have been reported. METHODS All cases of primary CNS tumors that were registered at Center for Disease Control and Prevention (CDC) were collected (1973-2007: urban Shanghai; 2003-2007: whole Shanghai city). Trends were analyzed using joinpoint analysis and rates were stratified by age, gender and region. Histological data were collected from both CDC and Huashan Hospital. RESULTS From 1973 to 2007, the five-year average incidence rate in urban Shanghai increased in both genders, especially in the elderly population. Joinpoint analysis showed the age-adjusted incidence rate for males increased first but then plateaued, whilst rates for females continued increasing over the 35 years. For the five-year status quo (2003-2007), rural had a higher age- adjusted incidence rate than urban populations, and females higher than males, especially those with advanced age. According to CDC (2003-2007) and Huashan Hospital (1951-2011), the two most common histological subtypes were neuroepithelial tumors (with male predominance) and meningiomas (with female predominance). CONCLUSIONS In Shanghai, a steadily increased incidence rate of primary CNS tumors was observed in general, and in the elderly and female population in particular.
Subsyndromes of chronic schizophrenia: a phenomenological study

Statistical grouping of clinical symptoms and signs is one method of classifying schizophrenia. This study attempted to find factors of symptoms generated through clinical ratings on three scales, namely the Scale for the Assessment of Positive Symptoms (SAPS), the Scale for the Assessment of Negative Symptoms (SANS) and the Comprehensive Psychopathological Rating Scale (CPRS) in a sample of 80 stable chronic schizophrenics diagnosed by DSM‐III‐R. It was found that the positive‐negative dichotomy could not be replicated. Inappropriate affect was found to be a clear misfit in the cluster of negative symptoms. Inclusion of CPRS led to the generation of a factor loading high on depressive and asthenic items, and this could be clearly distinguished from the factor of negative symptoms. This factor had not hitherto been identified.
Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

ABSTRACT An enzyme-linked immunofiltration assay (ELIFA) has been developed in order to estimate directly and specifically Thiobacillus ferrooxidans attachment on sulfide minerals. This method derives from the enzyme-linked immunosorbent assay but is performed on filtration membranes which allow the retention of mineral particles for a subsequent immunoenzymatic reaction in microtiter plates. The polyclonal antiserum used in this study was raised againstT. ferrooxidans DSM 583 and recognized cell surface antigens present on bacteria belonging to the genusThiobacillus. This antiserum and the ELIFA allowed the direct quantification of attached bacteria with high sensitivity (104 bacteria were detected per well of the microtiter plate). The mean value of bacterial attachment has been estimated to be about 105 bacteria mg−1 of pyrite at a particle size of 56 to 65 μm. The geometric coverage ratio of pyrite by T. ferrooxidans ranged from 0.25 to 2.25%. This suggests an attachment of T. ferrooxidans on the pyrite surface to well-defined limited sites with specific electrochemical or surface properties. ELIFA was shown to be compatible with the measurement of variable levels of adhesion. Therefore, this method may be used to establish adhesion isotherms of T. ferrooxidans on various sulfide minerals exhibiting different physicochemical properties in order to understand the mechanisms of bacterial interaction with mineral surfaces.
What Young Adolescents Think About Engineering

To explore young adolescents’ interest in engineering as a future career, we examined the influence of gender and grade level on participants’ (N = 197, aged 10–13) views of engineering. One group (107 students) viewed a brief engineering video and wrote why they felt the same or different about engineering following the video. Qualitative analyses revealed that some reported viewing engineering differently and more positively, although most did not want to be an engineer. Girls, more than boys, noted that engineers helped people. Six months after the video intervention, participants completed quantitative measures about engineering. Quantitative analyses comparing responses of participants who had seen the video, with those who had not, revealed that the video dispelled some stereotyped beliefs, but not others, with grade-level and gender effects. The findings highlight the importance of listening to adolescents’ views about engineering as a field and as a future career.
3-D model localization using high-resolution reconstruction of monocular image sequences

In this paper, we present a complete system for the recognition and localization of a three-dimensional (3-D) model from a sequence of monocular images with known motion. The originality of this system is twofold. First, it uses a purely 3-D approach, starting from the 3-D reconstruction of the scene and ending by the 3-D matching of the model. Second, unlike most monocular systems, we do not use token tracking to match successive images. Rather, subpixel contour matching is used to recover more precisely complete 3-D contours. In contrast with the token tracking approaches, which yield a representation of the 3-D scene based on disconnected segments or points, this approach provides us with a denser and higher level representation of the scene. The reconstructed contours are fused along successive images using a simple result derived from the Kalman filter theory. The fusion process increases the localization precision and the robustness of the 3-D reconstruction. Finally, corners are extracted from the 3-D contours. They are used to generate hypotheses of the model position, using a hypothesize-and-verify algorithm that is described in detail. This algorithm yields a robust recognition and precise localization of the model in the scene. Results are presented on infrared image sequences with different resolutions, demonstrating the precision of the localization as well as the robustness and the low computational complexity of the algorithms.
Antibody reactive with Porphyromonas gingivalis hemagglutinin in chronic and generalized aggressive periodontitis.

BACKGROUND Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions and its presence in subgingival plaque significantly increases the risk for periodontitis. We have previously shown that patients with aggressive forms of periodontitis that are seropositive for P. gingivalis have less attachment loss than those that are seronegative. This suggests that antibody reactive with antigens of P. gingivalis may be protective and decrease disease severity and extent. Recent studies in the murine abscess model and in the host antibody response in chronic periodontitis patients suggest that antibody reactive with P. gingivalis hemagglutinin may be an important protective antibody response. OBJECTIVES In this study, we tested the hypothesis that there was a significant relationship between antibody reactive with P. gingivalis hemagglutinin and measures of periodontal attachment loss. METHODS We determined the immunoglobulin G (IgG) antibody concentration reactive with recombinant P. gingivalis hemagglutinin in 117 chronic periodontitis and 90 generalized aggressive periodontitis patients. We also determined the IgG subclass distribution for antibody reactive with P. gingivalis hemagglutinin. RESULTS AND CONCLUSIONS We found IgG reactive with P. gingivalis hemagglutinin in both chronic periodontitis and generalized aggressive periodontitis patients. Most of this IgG antibody was of the IgG1 and IgG3 subclasses. Antibody reactive with P. gingivalis hemagglutinin, however, did not have a significant relationship with measures of periodontal attachment loss.
The influence of stem insertion rate on the porosity of the cement mantle of hip joint replacements

Abstract This study investigates the effect of stem insertion rate on the porosity of the cement mantle. An experimental protocol was developed to simulate the surgical technique of cementing a prosthetic stem into the medullary canal of the femur. Cement mantle specimens were produced for three different stem insertion rates. The presence of porosity in the cement mantle was investigated. Additionally, the mechanical strength of the bone cement was assessed. Increasing the stem insertion rate did not have a significant effect on the porosity distribution within the bulk cement mantle. However, for all stem insertion rates investigated, the porosity concentration increased significantly moving from the cement/pseudofemur interface through to the stem/cement interface. In all cases, the presence of porosity significantly decreased the mechanical behaviour of the bone cement. High porosity concentration at the stem/cement interface seems to be attributed also to the rheology of the cement during implant insertion. Nevertheless, the surgeon cannot influence the formation of porosity by changing the stem insertion rate.
Thermodynamics of Ligand Binding to P450 2B4 and P450eryF Studied by Isothermal Titration Calorimetry

Structural plasticity and cooperativity in ligand recognition are two key aspects of the catalytic diversity of cytochrome P450 enzymes. As more mammalian P450 crystal structures have emerged, computational modeling has become a major tool to predict drug metabolism and interactions. There is a need for real solution thermodynamic data to support modeling and crystallographic observations. Using isothermal titration calorimetry (ITC) we successfully evaluated the conformational flexibility of P450 2B4 in binding imidazole inhibitors of different size and chemistry and dissected the stoichiometry and energetics of ligand binding allostery in P450eryF. Thermodynamic signatures obtained by ITC nicely correlated with structural and modeling results. Thus, ITC is a powerful tool to study structure-function relationships in P450s.
From melody to lexical tone: Musical ability enhances specific aspects of foreign language perception

Previous research shows that music ability provides positive effects on language processing. This study aims at better clarifying the involvement of different linguistic subdomains in this cross-domain link, assessing whether or not musicality and music expertise enhance phonological and lexical tone processing of Mandarin Chinese. In two experiments different groups of adults and children with no previous experience in tonal languages, were invited to perform a same–different task trying to detect phonological and tonal variations in pairs of sequences of monosyllabic Mandarin Chinese words. Main results show that all subjects perform significantly better in detecting phonological variations rather than tonal ones. They also show that both melodic proficiency and music expertise are good predictors for a better tonal, but not phonological identification. Data lead to a model of music-to-language transfer effect in which musicality selectively affects linguistic intonation while leaving phonological processing substantially unaffected.
Comparison of 2-D calculations from periapical and occlusal radiographs versus 3-D calculations from CAT scans in determining bone support for cleft-adjacent teeth following early alveolar bone grafts.

OBJECTIVE This investigation was conducted to determine the agreement between three-dimensional (3-D) calculations from CAT scans and two-dimensional (2-D) calculations from standard dental radiographs in evaluating bone support for cleft-adjacent teeth after primary bone grafting. DESIGN This retrospective study utilized CAT scans and dental radiographs taken of the alveolar cleft in patients an average of 11 years after primary bone grafting. SETTING The subjects were patients treated by the Cleft Palate Team at Children's Memorial Hospital and Loyola University Medical Center, Chicago, Illinois. PATIENTS Fourteen UCLP patients (9 males, 5 females) agreed to participate in this study by undergoing CAT scan assessment of their alveolar cleft sites. They also had to have periapical or occlusal radiographs of the grafted cleft site taken within 6 months of the CAT scan. INTERVENTIONS All patients underwent primary lip repair, placement of a passive palatal plate, primary alveolar bone grafting (mean age 6.4 months), and palatoplasty before 1 year of age. Major tooth movement through final orthodontics was completed by the time of the radiographic assessment. MAIN OUTCOME MEASURES CAT scan sections were reformatted and reconstructed to three-dimensionally calculate the percentage of root covered by bone support for the 15 teeth adjacent to the graft cleft sites. Dental radiographs of the same teeth were also traced and digitized. Percentages of root supported by bone were also established using the dental radiographs by dividing the amount of root covered by bone, by the anatomic root length. RESULTS A paired, two-sample t test revealed no significant differences between the two methods of assessment, while linear regression showed a statistically significant correlation between the CAT scan assessment and the percentages found on the radiographs. CONCLUSIONS Routine dental radiographs were able to estimate the total 3-D bone support for the roots of cleft adjacent teeth as determined by CAT scan to a statistically significant degree when groups where compared. The clinical significance for evaluation of individual cases was less impressive with a wide range of variability and a level of agreement that required acceptance of differences up to 25%.
Design And Analysis of a Box Floater With Damping Plates For Floating Wind Turbine Platform

In the present study, a numerical and experimental study is conducted for a floating wind tower substructure of 5MW capacity. The design targets are cost effective, smaller draft than SPAR, simple to construct and install. The basic idea for shape design comes from the truss-spar which utilizes a large heaving plate for enhancing global performance. A 9-node HOBEM was applied for numerical parametric study because of its accuracy especially for sharp-edged shapes. A preliminary model test was conducted for verification of the numerical design. The numerical and model tests results were compared with same class spar type design
Cdc14B depletion leads to centriole amplification, and its overexpression prevents unscheduled centriole duplication

Centrosome duplication is tightly controlled in coordination with DNA replication. The molecular mechanism of centrosome duplication remains unclear. Previous studies found that a fraction of human proline-directed phosphatase Cdc14B associates with centrosomes. However, Cdc14B's involvement in centrosome cycle control has never been explored. Here, we show that depletion of Cdc14B by RNA interference leads to centriole amplification in both HeLa and normal human fibroblast BJ and MRC-5 cells. Induction of Cdc14B expression through a regulatable promoter significantly attenuates centriole amplification in prolonged S phase–arrested cells and proteasome inhibitor Z-L3VS–treated cells. This inhibitory function requires centriole-associated Cdc14B catalytic activity. Together, these results suggest a potential function for Cdc14B phosphatase in maintaining the fidelity of centrosome duplication cycle.
The Utility of Image-Based Point Clouds for Forest Inventory: A Comparison with Airborne Laser Scanning

Airborne Laser Scanning (ALS), also known as Light Detection and Ranging (LiDAR) enables an accurate three-dimensional characterization of vertical forest structure. ALS has proven to be an information-rich asset for forest managers, enabling the generation of highly detailed bare earth digital elevation models (DEMs) as well as estimation of a range of forest inventory attributes (including height, basal area, and volume). Recently, there has been increasing interest in the advanced processing of high spatial resolution digital airborne imagery to generate image-based point clouds, from which vertical information with similarities to ALS can be produced. Digital airborne imagery is typically less costly to acquire than ALS, is well understood by inventory practitioners, and in addition to enabling the derivation of height information, allows for visual interpretation of attributes that are currently problematic to estimate from ALS (such as species, health status, and maturity). At present, there are two limiting factors associated with the use of image-based point clouds. First, a DEM is required to normalize the image-based point cloud heights to aboveground heights; however DEMs with sufficient spatial resolution and vertical accuracy, particularly in forested areas, are usually only available from ALS data. The use of image-based point clouds may therefore be limited to those forest areas that already have an ALS-derived DEM. Second, image-based point clouds primarily characterize the outer envelope of the forest canopy, whereas ALS pulses penetrate the canopy and provide information on sub-canopy forest structure. The impact of these limiting factors on the estimation of forest inventory attributes has not been extensively researched and is not yet well understood. In this paper, we review the key similarities and differences between ALS data and image-based point clouds, summarize the results of current research related to the comparative use of these data for forest inventory attribute estimation, and highlight some outstanding research questions that should be addressed before any definitive recommendation can be made regarding the use of image-based point clouds for this application.
A classroom observation-based evaluation of elementary teachers use of technology in the classrooms in Turkey

Intensive studies have been carried out in relation to the teacher’s role and expectations of the integration of technology into education. In this framework, this study investigates the reason why teachers integrate technology into their lessons, the type of technology they use and how they integrate it into their lessons. Furthermore, this study aims to determine the indicators of technology use and the special conditions for certain applications. The data relating to teacher’s technology integrations into their lessons were obtained from classroom observations according to criteria determined after in-service training had been received. The sampling of the study comprised 32 elementary school teachers. During the observations, the observation form prepared within the scope of the project was used. Frequency tables were used to analyse the data. As a result, it was observed that the teachers who had received in service training purposefully integrated technology into their lessons and they generally understood how to select an appropriate technology for their particular teaching purpose. It was also observed that teachers new to the use of technology in the classrooms used conventional methods to integrate technology in their teaching.
Beta cell differentiation during early human pancreas development.

Understanding gene expression profiles during early human pancreas development is limited by comparison to studies in rodents. In this study, from the inception of pancreatic formation, embryonic pancreatic epithelial cells, approximately half of which were proliferative, expressed nuclear PDX1 and cytoplasmic CK19. Later, in the fetal pancreas, insulin was the most abundant hormone detected during the first trimester in largely non-proliferative cells. At sequential stages of early fetal development, as the number of insulin-positive cell clusters increased, the detection of CK19 in these cells diminished. PDX1 remained expressed in fetal beta cells. Vascular structures were present within the loose stroma surrounding pancreatic epithelial cells during embryogenesis. At 10 weeks post-conception (w.p.c.), all clusters containing more than ten insulin-positive cells had developed an intimate relationship with these vessels, compared with the remainder of the developing pancreas. At 12-13 w.p.c. , human fetal islets, penetrated by vasculature, contained cells independently immunoreactive for insulin, glucagon, somatostatin and pancreatic polypeptide (PP), coincident with the expression of maturity markers prohormone convertase 1/3 (PC1/3), islet amyloid polypeptide, Chromogranin A and, more weakly, GLUT2. These data support the function of fetal beta cells as true endocrine cells by the end of the first trimester of human pregnancy.