Miracle-dz/UniFAR-FTUs huggingface dataset:
Nanodiamond-enhanced MRI

Nanodiamonds are of interest as nontoxic substrates for targeted drug delivery and as highly biostable fluorescent markers for cellular tracking. Beyond optical techniques however, options for noninvasive imaging of nanodiamonds in vivo are severely limited. Here, we demonstrate that the Overhauser effect, a proton-electron double resonance technique developed to detect free radicals in vivo, can enable high contrast magnetic resonance imaging (MRI) of nanodiamonds in water at room temperature and ultra-low magnetic field. The technique transfers spin polarization from paramagnetic impurities at nanodiamond surfaces to $^1$H spins in the surrounding water solution, creating MRI contrast on-demand. We further examine the conditions required for maximum enhancement as well as the ultimate sensitivity of the technique. The ability to perform continuous hyperpolarization via the Overhauser mechanism, in combination with excellent in vivo stability, raises the possibility of performing noninvasive tracking of nanodiamonds over indefinitely long periods of time.
A cognitive approach to elicit verbal and nonverbal cues to deceit

Dishonesty is ubiquitous in our world. The news is frequently filled with high-profile cases of corporate fraud, large-scale corruption, lying politicians, and the hypocrisy of public figures. On a smaller scale, ordinary people often cheat, lie, misreport their taxes, and mislead others in their daily life. Despite such prevalence of cheating, corruption, and concealment, people typically consider themselves to be honest, and often believe themselves to be more moral than most others. This book aims to resolve this paradox by addressing the question of why people are dishonest all too often. What motivates dishonesty, and how are people able to perceive themselves as moral despite their dishonest behaviour? What personality and interpersonal factors make dishonesty more likely? And what can be done to recognize and reduce dishonesty? This is a fascinating overview of state-of-the-art research on dishonesty, with prominent scholars offering their views to clarify the roots of dishonesty.
Large-scale shifts in phytoplankton groups in the Equatorial Pacific during ENSO cycles

Abstract. The El Nino Southern Oscillation (ENSO) drives important changes in the marine productivity of the Equatorial Pacific, in particular during major El Nino/La Nina transitions. Changes in environmental conditions associated with these climatic events also likely impact phytoplankton composition. In this work, the distribution of four major phytoplankton groups (nanoeucaryotes, Prochlorococcus, Synechococcus, and diatoms) was examined between 1996 and 2007 by applying the PHYSAT algorithm to the ocean color data archive from the Ocean Color and Temperature Sensor (OCTS) and Sea-viewing Wide Field-of-view Sensor (SeaWiFS). Coincident with the decrease in chlorophyll concentrations, a large-scale shift in the phytoplankton composition of the Equatorial Pacific, that was characterized by a decrease in Synechococcus and an increase in nanoeucaryote dominance, was observed during the early stages of both the strong El Nino of 1997 and the moderate El Nino of 2006. A significant increase in diatoms dominance was observed in the Equatorial Pacific during the 1998 La Nina and was associated with elevated marine productivity. An analysis of the environmental variables using a coupled physical-biogeochemical model (NEMO-PISCES) suggests that the Synechococcus dominance decrease during the two El Nino events was associated with an abrupt decline in nutrient availability (−0.9 to −2.5 μM NO3 month−1). Alternatively, increased nutrient availability (3 μM NO3 month−1) during the 1998 La Nina resulted in Equatorial Pacific dominance diatom increase. Despite these phytoplankton community shifts, the mean composition is restored after a few months, which suggests resilience in community structure.
Inhibition of miR-130 a expression promotes metastasis of lung squamous carcinoma cells

Aims: In this study, we aim to investigate the expression of miR-130a in human lung cancer tissues, to examine the association between miR-130a expressions and the clinicopathologic features of squamous carcinoma, and to determine the biological function of miR-130a in lung cancer. Methods: The expression of miR-130a in lung cancer tissues and their matched adjacent para-cancerous tissues was analyzed by Realtime PCR. A549 and Calu-3 cells were transfected with miR-130a mimics or scramble (NC), and the proliferation and migration of these cells were tested by MTT and CCK8 analysis. Cell cycle was detected by flow cytometry, and the expression of cell cycle-related proteins as well as epithelial mesenchymal transition (EMT)-related proteins was analyzed by Western blot. Results: The expression levels of miR-130a were significantly reduced in lung cancer tissues compared with adjacent para-cancerous tissues, and the expression levels of miR-130a were decreased in patients with lymph node metastasis compared with that without lymph node metastasis. However, changes of miR-130a expression were independent of sex, age, the size of tumor, lesion location, degrees of differentiation and the depth of tumor invasion. In in vitro experiments, the proliferation and migration of A549 and Calu-3 transfected with miR-130a mimics were significantly inhibited compared with NC. MiR-130a overexpression induced a G1/S arrest. CyclinD1, CyclinE1 and EMT-related protein E-Cadherin was upregulated while Vimentin was down regulated in miR-130a mimic transfected cells. Conclusions: These findings suggest that marked downregulation of the miR-130a may play a role in the development and invasion and metastasis of lung cancers. MiR-130a may be a potential therapeutic target of lung cancer.
Open-ended dielectric-filled waveguide antenna for underwater usage

In wireless short-range underwater communication a trend towards higher operating frequencies and bandwidth can be noticed in order to increase channel capacity and throughput. For launching electromagnetic waves typically low-directive wire antennas are employed. In this work a new kind of underwater antenna is presented for the 2.4GHz industrial, scientific and medical (ISM) band, based on a dielectric filled open-ended waveguide. The antenna is designed as impedance matching transformer allowing a connection of conventional 50 Ω coaxial cables. The measured 10 dB bandwidth in fresh water is approx. 550MHz (22 %). Compared to a conventional monopole antenna an increase in receive power of approx. 14 dB can be achieved.
The Reversible Sex of Gonochoristic Fish: Insights and Consequences

Fish sex reversal is a means to understand sex determination and differentiation, but it is also used to control sex in aquaculture. This review discusses sex reversal in gonochoristic fish, with the coexistence of genetic and environmental influences. The different periods of fish sensitivity to sex reversal treatments are presented with the mechanisms implicated. The old players of sex differentiation are revisited with transcriptome data and loss of function studies following hormone- or temperature-induced sex reversal. We also discuss whether cortisol is the universal mediator of sex reversal in fish due to its implication in ovarian meiosis and 11KT increase. The large plasticity in fish for sex reversal is also evident in the brain, with a reversibility existing even in adulthood. Studies on epigenetics are presented, since it links the environment, gene expression, and sex reversal, notably the association of DNA methylation in sex reversal. Manipulations with exogenous factors reverse the primary sex in many fish species under controlled conditions, but several questions arise on whether this can occur under wild conditions and what is the ecological significance. Cases of sex reversal in wild fish populations are shown and their fitness and future perspectives are discussed.
The classification of normal screening mammograms

Rationale and objectives: To understand how breast screen readers classify the difficulty of normal screening mammograms using common lexicon describing normal appearances. Cases were also assessed on their suitability for a single reader strategy. Materials and Methods: 15 breast readers were asked to interpret a test set of 29 normal screening mammogram cases and classify them by rating the difficulty of the case on a five-point Likert scale, identifying the salient features and assessing their suitability for single reading. Using the False Positive Fractions from a previous study, the 29 cases were classified into 10 "low", 10 "medium" and nine "high" difficulties. Data was analyzed with descriptive statistics. Spearman's correlation was used to test the strength of association between the difficulty of the cases and the readers’ recommendation for single reading strategy. Results: The ratings from readers in this study corresponded to the known difficulty level of cases for the 'low' and 'high' difficulty cases. Uniform ductal pattern and density, symmetrical mammographic features and the absence of micro-calcifications were the main reasons associated with 'low' difficulty cases. The 'high' difficulty cases were described as having ‘dense breasts’. There was a statistically significant negative correlation between the difficulty of the cases and readers’ recommendation for single reading (r = -0.475, P = 0.009). Conclusion: The findings demonstrated potential relationships between certain mammographic features and the difficulty for readers to classify mammograms as 'normal'. The standard Australian practice of double reading was deemed more suitable for most cases. There was an inverse moderate association between the difficulty of the cases and the recommendations for single reading.
Intrinsic disorder as a generalizable strategy for the rational design of highly responsive, allosterically cooperative receptors

Significance Control over the sensitivity with which biomolecular receptors respond to small changes in the concentration of their target ligand is crucial to many cellular processes and likely could be of value in many biotechnologies. In nature, this control is often achieved using “Hill-type” allosteric cooperativity, an elegant mechanism that has, unfortunately, hitherto proven difficult to achieve via generalizable design strategies. In response, we demonstrate here a quantitative and apparently versatile means of rationally introducing this useful mechanism into a range of normally noncooperative receptors. We achieve in the best of our examples cooperativity, and thus sensitivity, experimentally indistinguishable from the theoretically expected maximum. Control over the sensitivity with which biomolecular receptors respond to small changes in the concentration of their target ligand is critical for the proper function of many cellular processes. Such control could likewise be of utility in artificial biotechnologies, such as biosensors, genetic logic gates, and “smart” materials, in which highly responsive behavior is of value. In nature, the control of molecular responsiveness is often achieved using “Hill-type” cooperativity, a mechanism in which sequential binding events on a multivalent receptor are coupled such that the first enhances the affinity of the next, producing a steep, higher-order dependence on target concentration. Here, we use an intrinsic-disorder–based mechanism that can be implemented without requiring detailed structural knowledge to rationally introduce this potentially useful property into several normally noncooperative biomolecules. To do so, we fabricate a tandem repeat of the receptor that is destabilized (unfolded) via the introduction of a long, unstructured loop. The first binding event requires the energetically unfavorable closing of this loop, reducing its affinity relative to that of the second binding event, which, in contrast occurs at a preformed site. Using this approach, we have rationally introduced cooperativity into three unrelated DNA aptamers, achieving in the best of these a Hill coefficient experimentally indistinguishable from the theoretically expected maximum. The extent of cooperativity and thus the steepness of the binding transition are, moreover, well modeled as simple functions of the energetic cost of binding-induced folding, speaking to the quantitative nature of this design strategy.
Fasudil Protects the Heart against Ischemia-Reperfusion Injury by Attenuating Endoplasmic Reticulum Stress and Modulating SERCA Activity: The Differential Role for PI3K/Akt and JAK2/STAT3 Signaling Pathways

Disordered calcium homeostasis can lead to endoplasmic reticulum (ER) stress. Our previous data showed that time course activation of ER stress contributes to time-related increase in ischemia-reperfusion (I/R) injury. However, it has not been tested whether PI3K/Akt and JAK2/STAT3 pathways play differential roles in reducing ER stress to protect the heart. In the present study, using fasudil which is a specific inhibitor of ROCK, we aimed to investigate whether improved SERCA expression and activity accounts for reduced ER stress by ROCK inhibition, specifically whether PI3K/Akt and JAK2/STAT3 pathways are differentially involved in modulating SERCA activity to reduce ER stress and hence I/R injury. The results showed that during the reperfusion period following 45 min of coronary ligation the infarct size (IS) increased from 3 h of reperfusion (45.4±5.57%) to 24 h reperfusion (64.21±5.43, P<0.05), which was associated with ER stress dependent apoptosis signaling activation including CHOP, Caspase-12 and JNK (P<0.05, respectively). The dynamic ER stress activation was also related to impaired SERCA activity at 24 h of reperfusion. Administration of fasudil at 10 mg/Kg significantly attenuated ROCK activation during reperfusion and resulted in an improved SERCA activity which was closely associated with decreases in temporal activation of ER stress and IS changes. Interestingly, while both PI3K/Akt and JAK2/STAT3 signaling pathways played equal role in the protection offered by ROCK inhibition at 3 h of reperfusion, the rescued SERCA expression and activity at 24 h of reperfusion by fasudil was mainly due to JAK2/STAT3 activation, in which PI3K/Akt signaling shared much less roles.
Foraging Blainville's beaked whales (Mesoplodon densirostris) produce distinct click types matched to different phases of echolocation

SUMMARY Blainville's beaked whales (Mesoplodon densirostris Blainville) echolocate for prey during deep foraging dives. Here we use acoustic tags to demonstrate that these whales, in contrast to other toothed whales studied, produce two distinct types of click sounds during different phases in biosonar-based foraging. Search clicks are emitted during foraging dives with inter-click intervals typically between 0.2 and 0.4 s. They have the distinctive form of an FM upsweep (modulation rate of about 110 kHz ms-1) with a -10 dB bandwidth from 26 to 51 kHz and a pulse length of 270 μs, somewhat similar to chirp signals in bats and Cuvier's beaked whales (Ziphius cavirostris Cuvier), but quite different from clicks of other toothed whales studied. In comparison, the buzz clicks, produced in short bursts during the final stage of prey capture, are short (105 μs) transients with no FM structure and a -10 dB bandwidth from 25 to 80 kHz or higher. Buzz clicks have properties similar to clicks reported from large delphinids and hold the potential for higher temporal resolution than the FM clicks. It is suggested that the two click types are adapted to the separate problems of target detection and classification versus capture of low target strength prey in a cluttered acoustic environment.
Comparison of Shedding Characteristics of Seasonal Influenza Virus (Sub)Types and Influenza A(H1N1)pdm09; Germany, 2007–2011

Background Influenza viral shedding studies provide fundamental information for preventive strategies and modelling exercises. We conducted a prospective household study to investigate viral shedding in seasonal and pandemic influenza between 2007 and 2011 in Berlin and Munich, Germany. Methods Study physicians recruited index patients and their household members. Serial nasal specimens were obtained from all household members over at least eight days and tested quantitatively by qRT-PCR for the influenza virus (sub)type of the index patient. A subset of samples was also tested by viral culture. Symptoms were recorded daily. Results We recruited 122 index patients and 320 household contacts, of which 67 became secondary household cases. Among all 189 influenza cases, 12 were infected with seasonal/prepandemic influenza A(H1N1), 19 with A(H3N2), 60 with influenza B, and 98 with A(H1N1)pdm09. Nine (14%) of 65 non-vaccinated secondary cases were asymptomatic/subclinical (0 (0%) of 21 children, 9 (21%) of 44 adults; p = 0.03). Viral load among patients with influenza-like illness (ILI) peaked on illness days 1, 2 or 3 for all (sub)types and declined steadily until days 7–9. Clinical symptom scores roughly paralleled viral shedding dynamics. On the first day prior to symptom onset 30% (12/40) of specimens were positive. Viral load in 6 asymptomatic/subclinical patients was similar to that in ILI-patients. Duration of infectiousness as measured by viral culture lasted approximately until illness days 4–6. Viral load did not seem to be influenced by antiviral therapy, age or vaccination status. Conclusion Asymptomatic/subclinical infections occur infrequently, but may be associated with substantial amounts of viral shedding. Presymptomatic shedding may arise in one third of cases, and shedding characteristics appear to be independent of (seasonal or pandemic) (sub)type, age, antiviral therapy or vaccination; however the power to find moderate differences was limited.
Trends in the quality of work presented at the society of british neurological surgeons meetings: 1975 to 2010

Abstract Background: The quality of scientific publications in clinical journals is well studied but the quality of work presented at medical conferences less so. Aims: To describe trends in the quality of presentations at the Society of British Neurological Surgeons [SBNS] conference between 1975 and 2010 and the factors associated with higher quality work in order to consider what might improve publication rates. Methods: Analysis was conducted in 5-year time periods (i.e. 1975–1979, 1985–1989, 1995–1999, 2005–2009). Published abstracts were used to identify conference presentations. Quality metrics included level of evidence of the presentation and eventual publication within 5 years. Publication 5-year citation count and destination journal impact factor were further used to assess publication quality. Statistical analysis was carried out using SPSS. Results: Of the 1711 presentations in total, 479 (28%) were published. The British Journal of Neurosurgery (93, 19%) was the favoured destination. Although the total number of publications has increased, given the increase in the number of presentations, the proportion of work published has decreased (80/179; 45% in the 1970s to 113/721; 16% in the 2000s). The growth in the impact factor of published work was better than that found in leading neurosurgical journals, but lower than for leading medical journals. In a multivariate model, presentations using a higher level of evidence increased the likelihood of publication (AOR 6.7 95% CI 3.7, 12.1), whilst presenting at conferences after the 1970s reduced the likelihood of publication; 1985–1989 (AOR 0.3, 95% CI 0.2, 0.4), 1995–1999 (0.4, 95% CI 0.3, 0.7) and 2005–2009 (0.1, 95% CI 0.1, 0.2). Conclusion: SBNS conferences today contain more presentations and yield more publications than ever before. However, the increased volume may dilute the quality of work presented.
Suicidal Behavior and Haplotypes of the Dopamine Receptor Gene (DRD2) and ANKK1 Gene Polymorphisms in Patients with Alcohol Dependence – Preliminary Report

Suicide is a significant public health issue and a major cause of death throughout the world. According to WHO it accounts for almost 2% of deaths worldwide. The etiology of suicidal behavior is complex but the results of many studies suggest that genetic determinants are of significant importance. In our study,- we have analyzed selected SNPs polymorphisms in the DRD2 and ANKK1 genes in patients with alcohol dependence syndrome (169 Caucasian subjects) including a subgroup of individuals (n = 61) who have experienced at least one suicide attempt. The aim of the study was to verify if various haplotypes of selected genes, comprising Taq1A, Taq1B, and Taq1D single nucleotide polymorphisms (SNP), play any role in the development of alcohol dependence and suicidal behavior. The control group comprised 157 unrelated individuals matched for ethnicity, gender,- and age and included no individuals with mental disorders. All subjects were recruited in the North West region of Poland. The study showed that alcohol dependent subjects with a history of at least one suicidal attempt were characterized by a significantly higher frequency of the T-G-A2 haplotype when compared to individuals in whom alcohol dependence was not associated with suicidal behavior (p = 0.006). It appears that studies based on identifying correlation between SNPs is the future for research on genetic risk factors that contribute to the development of alcohol addiction and other associated disorders. To sum up, there is a necessity to perform further research to explain dependencies between the dopaminergic system, alcohol use disorders and suicidal behavior.
Is sunlight exposure a risk factor for age-related macular degeneration? A systematic review and meta-analysis

Background Epidemiologists have recently investigated sunlight exposure as a risk factor for age-related macular degeneration (AMD), but there remains an ongoing dispute over this association due to insufficient evidence and unreliable data. Objectives To analyse comprehensively the epidemiological literature concerning the association between AMD and sunlight exposure. Methods We systematically reviewed the epidemiological literature concerning the association between AMD and sunlight exposure. An electronic search was performed of PubMed, Web of Science and CNKI, which was supplemented by hand searching. The selection of studies, data abstraction and quality assessment were performed independently by three reviewers. After these steps, we performed a random-effects meta-analysis, followed by subgroup analysis and sensitivity analysis, including a random-effects meta-regression for study-speciﬁc covariates. Results Fourteen studies were identified. Twelve studies identified an increasing risk of AMD with greater sunlight exposure, six of which reported significant risks. The pooled OR was 1.379 (95% CI 1.091 to 1.745). The subgroup of non-population-based studies revealed a significant risk (OR 2.018, 1.248 to 3.265, p=0.004). We identified the gross domestic product (GDP) per capita (p=0.048), but not the latitude (p=0.21), as a factor that led to heterogeneity according to the meta-regression. Conclusions The epidemiological literature published to date indicates that individuals with more sunlight exposure are at a significantly increased risk of AMD. The OR significantly decreased with increasing GDP per capita.