Miracle-dz/UniFAR-FTUs huggingface dataset:
Chitosan-DNA nanoparticles delivered by intrabiliary infusion enhance liver-targeted gene delivery

The goal of this study was to examine the efficacy of liver-targeted gene delivery by chitosan-DNA nanoparticles through retrograde intrabiliary infusion (RII). The transfection efficiency of chitosan-DNA nanoparticles, as compared with PEI-DNA nanoparticles or naked DNA, was evaluated in Wistar rats by infusion into the common bile duct, portal vein, or tail vein. Chitosan-DNA nanoparticles administrated through the portal vein or tail vein did not produce detectable luciferase expression. In contrast, rats that received chitosan-DNA nanoparticles showed more than 500 times higher luciferase expression in the liver 3 days after RII; and transgene expression levels decreased gradually over 14 days. Luciferase expression in the kidney, lung, spleen, and heart was negligible compared with that in the liver. RII of chitosan-DNA nanoparticles did not yield significant toxicity and damage to the liver and biliary tree as evidenced by liver function analysis and histopathological examination. Luciferase expression by RII of PEI-DNA nanoparticles was 17-fold lower than that of chitosan-DNA nanoparticles on day 3, but it increased slightly over time. These results suggest that RII is a promising routine to achieve liver-targeted gene delivery by non-viral nanoparticles; and both gene carrier characteristics and mode of administration significantly influence gene delivery efficiency.
Microbial Diversity in Ultra-High-Pressure Rocks and Fluids from the Chinese Continental Scientific Drilling Project in China

ABSTRACT Microbial communities in ultra-high-pressure (UHP) rocks and drilling fluids from the Chinese Continental Scientific Drilling Project were characterized. The rocks had a porosity of 1 to 3.5% and a permeability of ∼0.5 mDarcy. Abundant fluid and gas inclusions were present in the minerals. The rocks contained significant amounts of Fe2O3, FeO, P2O5, and nitrate (3 to 16 ppm). Acridine orange direct counting and phospholipid fatty acid analysis indicated that the total counts in the rocks and the fluids were 5.2 × 103 to 2.4 × 104 cells/g and 3.5 × 108 to 4.2 × 109 cells/g, respectively. Enrichment assays resulted in successful growth of thermophilic and alkaliphilic bacteria from the fluids, and some of these bacteria reduced Fe(III) to magnetite. 16S rRNA gene analyses indicated that the rocks were dominated by sequences similar to sequences of Proteobacteria and that most organisms were related to nitrate reducers from a saline, alkaline, cold habitat; however, some phylotypes were either members of a novel lineage or closely related to uncultured clones. The bacterial communities in the fluids were more diverse and included Proteobacteria, Bacteroidetes, gram-positive bacteria, Planctomycetes, and Candidatus taxa. The archaeal diversity was lower, and most sequences were not related to any known cultivated species. Some archaeal sequences were 90 to 95% similar to sequences recovered from ocean sediments or other subsurface environments. Some archaeal sequences from the drilling fluids were >93% similar to sequences of Sulfolobus solfataricus, and the thermophilic nature was consistent with the in situ temperature. We inferred that the microbes in the UHP rocks reside in fluid and gas inclusions, whereas those in the drilling fluids may be derived from subsurface fluids.
Forkhead Transcription Factors Inhibit Vascular Smooth Muscle Cell Proliferation and Neointimal Hyperplasia*[boxs]

Vascular smooth muscle cell (VSMC) proliferation and migration contribute significantly to atherosclerosis, postangioplasty restenosis, and transplant vasculopathy. Forkhead transcription factors belonging to the FoxO subfamily have been shown to inhibit growth and cell cycle progression in a variety of cell types. We hypothesized that forkhead proteins may play a role in VSMC biology. Under in vitro conditions, platelet-derived growth factor (PDGF)-BB, tumor necrosis factor-α, and insulin-like growth factor 1 stimulated phosphorylation of FoxO in human coronary artery smooth muscle cells via MEK1/2 and/or phosphatidylinositol 3-kinase-dependent signaling pathways. PDGF-BB, tumor necrosis factor-α, and insulin-like growth factor 1 treatment resulted in the nuclear exclusion of FoxO, whereas PDGF-BB alone down-regulated the FoxO target gene, p27kip1, and enhanced cell survival and progression through the cell cycle. These effects were abrogated by overexpression of a constitutively active, phosphorylation-resistant mutant of the FoxO family member, TM-FKHRL1. The anti-proliferative effect of TM-FKHRL1 was partially reversed by small interfering RNA against p27kip1. In a rat balloon carotid arterial injury model, adenovirus-mediated gene transfer of FKHRL1 caused an increase in the expression of p27kip1 in the VSMC and inhibition of neointimal hyperplasia. These data suggest that FoxO activity inhibits VSMC proliferation and activation and that this signaling axis may represent a therapeutic target in vasculopathic disease states.
Microarray gene expression profiling reveals antioxidant-like effects of angiotensin II inhibition in atherosclerosis

Reactive oxygen species (ROS) is a significant feature of atherosclerosis but the impact of ROS on atherogenesis is not clear since antioxidants such as vitamin E have little effect on atherosclerosis development in vivo. To investigate the role of ROS in atherosclerosis, we used ApoE-deficient mice, and compared the treatment effect of the antioxidant vitamin E with that of the angiotensin-converting enzyme (ACE) inhibitor, captopril, because angiotensin II is a major source of ROS in the vasculature. Dihydroethidium (DHE) staining demonstrated that vitamin E and captopril both prevented the atherosclerosis-induced increase in aortic superoxide content. In contrast, seven months of vitamin E treatment retarded the development of atherosclerotic lesions by only 45.8 ± 11.5% whereas captopril reduced the aortic plaque area by 88.1 ± 7.5%. To discriminate between vitamin E-sensitive and -insensitive effects of ACE inhibition, we performed whole genome microarray gene expression profiling. Gene ontology (GO) and immunohistology analyses showed that vitamin E and captopril prevented atherosclerosis-related changes of aortic intima and media genes. However, vitamin E did not reduce the expression of probe sets detecting the aortic recruitment of pro-inflammatory immune cells while immune cell-specific genes were normalized by captopril treatment. Moreover, vitamin E did not prevent the atherosclerosis-dependent down-regulation of perivascular nerve-specific genes, which were preserved in captopril-treated aortas. Taken together, our study detected antioxidant vitamin E-like effects of angiotensin II inhibition in atherosclerosis treatment regarding preservation of aortic intima and media genes. Additional vitamin E-insensitive effects targeting atherosclerosis-enhancing aortic immune cell recruitment and perivascular nerve degeneration could account for the stronger anti-atherogenic activity of ACE inhibition compared to vitamin E.
Advancing Trust Negotiations for Collaborative Tasks-Position Paper -

Sharing sensitive information across security domains is an important requirement for knowledge intensive collaborative work, but such sharing may impose risks on security and privacy integrity. Research has increasingly recognized the role of trust in minimizing the risk of information sharing across untrusted or unknown entities. To this extent, trust negotiations[1][3][15][20] have emerged as effective protocols to verify trust of unknown parties’ based on tangible properties. Trust management approaches have been designed to support business oriented interactions, and more in general, any type of interaction that involves exchanging sensitive resources for a shared need among – usuallytwo parties.
Effect of platelet additive solution on bacterial dynamics and their influence on platelet quality in stored platelet concentrates

BACKGROUND: Platelet additive solutions (PASs) are an alternative to plasma for the storage of platelet concentrates (PCs). However, little is known about the effect of PAS on the growth dynamics of contaminant bacteria. Conversely, there have been no studies on the influence of bacteria on platelet (PLT) quality indicators when suspended in PAS.
SOME AVERAGING AND STABILITY RESULTS FOR RANDOM DIFFERENTIAL EQUATIONS

This paper concerns differential equations which contain strongly mixing random processes (processes for which the “past” and the “future” are asymptotically independent). When the “rate” of mixing is rapid relative to the rate of change of the solution process, information about the behavior of the solution is obtained. Roughly, the results fall into three categories:1. Quite generally, the solution process is well approximated by a deterministic trajectory, over a finite time interval. 2. For more restricted systems, this approximation extends to the infinite interval $[ {0,\infty } ),3$. Conditions for the asymptotic stability of $\dot x = AX$, where A is an $n \times n$ matrix-valued random process, are obtained.
SwarmViz: An open-source visualization tool for Particle Swarm Optimization

Particle Swarm Optimization (PSO) is a metaheuristic for solving high dimensional optimization problems. Due to the large number of dimensions usually employed with PSO, it is not trivial to visualize and monitor the progress of the algorithm. Because of this, adjusting the parameters that govern the dynamics of the swarm for a specific problem becomes challenging. In this article, we present SwarmViz, an open-source visualization tool for PSO. Through SwarmViz, users are able to set up PSO experiments on canonical benchmark functions or input data from external experiments (e.g., learning robotic controllers), and to visualize the optimization process with state-of-the-art visualization tools. SwarmViz has two main goals. First, to enable researchers to monitor the progress of their specific optimization problem and adjust the relevant PSO parameters. Second, to give a visual insight about PSO to students in the scope of teaching optimization techniques. We demonstrate the features of the software through examples on well-known numerical benchmark functions and a case study on the optimization of a robotic controller.
' s personal copy Independent component analysis and clustering improve signal-to-noise ratio for statistical analysis of event-related potentials

Objective: To evaluate the effectiveness of a new method of using Independent Component Analysis (ICA) and k-means clustering to increase the signal-to-noise ratio of Event-Related Potential (ERP) measurements while permitting standard statistical comparisons to be made despite the inter-subject variations characteristic of ICA. Methods: Per-subject ICA results were used to create a channel pool, with unequal weights, that could be applied consistently across subjects. Signals derived from this and other pooling schemes, and from unpooled electrodes, were subjected to identical statistical analysis of the N170 own-face effect in a Joe/No Joe face recognition paradigm wherein participants monitored for a target face (Joe) presented amongst other unfamiliar faces and their own face. Results between the Joe, unfamiliar face and own face conditions were compared using Cohen’s d statistic (square root of signal-to-noise ratio) to measure effect size. Results: When the own-face condition was compared to the Joe and unfamiliar-face conditions, the channel map method increased effect size by a factor ranging from 1.2 to 2.2. These results stand in contrast to previous findings, where conventional pooling schemes failed to reveal an N170 effect to the own-face stimulus (Tanaka JW, Curran T, Porterfield A, Collins D. The activation of pre-existing and acquired face representations: the N250 ERP as an index of face familiarity. J Cogn Neurosci 2006;18:1488–97). Consistent with conventional pooling schemes, the channel map approach showed no reliable differences between the Joe and Unfamiliar face conditions, yielding a decrease in effect size ranging from 0.13 to 0.75. Conclusions: By increasing the signal-to-noise ratio in the measured waveforms, the channel pool method demonstrated an enhanced sensitivity to the neurophysiological response to own-face relative to other faces. Significance: By overcoming the characteristic inter-subject variations of ICA, this work allows classic ERP analysis methods to exploit the improved signal-to-noise ratio obtainable with ICA. 2007 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.
Detection and Verification of Mycobacterium avium subsp. paratuberculosis in Fresh Ileocolonic Mucosal Biopsy Specimens from Individuals with and without Crohn's Disease

ABSTRACT Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows' milk in the United Kingdom and, potentially, elsewhere. Water supplies are also at risk. The involvement of M. avium subsp. paratuberculosis in Crohn's disease (CD) in humans has been uncertain because of the substantial difficulties in detecting this pathogen. In its Ziehl-Neelsen staining-negative form, M. avium subsp. paratuberculosis is highly resistant to chemical and enzymatic lysis. The present study describes the development of optimized sample processing and DNA extraction procedures with fresh human intestinal mucosal biopsy specimens which ensure access to M. avium subsp. paratuberculosis DNA and maximize detection of these low-abundance pathogens. Also described are two nested PCR methodologies targeted at IS900, designated IS900[L/AV] and IS900[TJ1-4], which are uniquely specific for IS900. Detection of M. avium subsp. paratuberculosis in mucosal biopsy specimens was also evaluated by using mycobacterial growth indicator tube (MGIT) cultures (Becton Dickinson). IS900[L/AV] PCR detected M. avium subsp. paratuberculosis in 34 of 37 (92%) patients with CD and in 9 of 34 (26%) controls without CD (noninflammatory bowel disease [nIBD] controls) (P = 0.0002; odds ratio = 3.47). M. avium subsp. paratuberculosis was detected by IS900[L/AV] PCR in MGIT cultures after 14 to 88 weeks of incubation in 14 of 33 (42%) CD patients and 3 of 33 (9%) nIBD controls (P = 0.0019; odds ratio = 4.66). Nine of 15 (60%) MGIT cultures of specimens from CD patients incubated for more than 38 weeks were positive for M. avium subsp. paratuberculosis. In each case the identity of IS900 from M. avium subsp. paratuberculosis was verified by amplicon sequencing. The rate of detection of M. avium subsp. paratuberculosis in individuals with CD is highly significant and implicates this chronic enteric pathogen in disease causation.
RecG Directs DNA Synthesis during Double-Strand Break Repair

Homologous recombination provides a mechanism of DNA double-strand break repair (DSBR) that requires an intact, homologous template for DNA synthesis. When DNA synthesis associated with DSBR is convergent, the broken DNA strands are replaced and repair is accurate. However, if divergent DNA synthesis is established, over-replication of flanking DNA may occur with deleterious consequences. The RecG protein of Escherichia coli is a helicase and translocase that can re-model 3-way and 4-way DNA structures such as replication forks and Holliday junctions. However, the primary role of RecG in live cells has remained elusive. Here we show that, in the absence of RecG, attempted DSBR is accompanied by divergent DNA replication at the site of an induced chromosomal DNA double-strand break. Furthermore, DNA double-stand ends are generated in a recG mutant at sites known to block replication forks. These double-strand ends, also trigger DSBR and the divergent DNA replication characteristic of this mutant, which can explain over-replication of the terminus region of the chromosome. The loss of DNA associated with unwinding joint molecules previously observed in the absence of RuvAB and RecG, is suppressed by a helicase deficient PriA mutation (priA300), arguing that the action of RecG ensures that PriA is bound correctly on D-loops to direct DNA replication rather than to unwind joint molecules. This has led us to put forward a revised model of homologous recombination in which the re-modelling of branched intermediates by RecG plays a fundamental role in directing DNA synthesis and thus maintaining genomic stability.
Towards a new treatment paradigm for ruptured blood blister-like aneurysms of the internal carotid artery? A rapid systematic review

Background and purpose Owing to their peculiar features and rare occurrence, ruptured blood blister-like aneurysms (BBAs) of the internal carotid artery are challenging by both surgical and endovascular approaches and their proper management is uncertain. We therefore aimed to define the currently optimal treatment of ruptured BBAs in terms of mortality, outcome, rebleeding, and recurrence. Method An in-depth search of electronic databases, gray literature and internet resources for ruptured BBAs was performed and complemented by data retrieval during neurosurgical congresses. Clinical and radiological characteristics, intervention details, outcomes, and the impact factor of the source journal were pooled. Results The pooled cohort comprised 311 patients. Neither surgical nor endovascular methods had an impact on clinical outcome, aneurysm regrowth, remote bleeding, or complication rate. By contrast, aneurysm clipping was a predictor of intraoperative bleeding (OR 6.5; 95% CI 1.2 to 34.3), and stent-assisted coiling increased the likelihood of a second treatment (OR 4.1; 95% CI 1.3 to 13.1), its conversion to another modality (OR 4.7; 95% CI 1.4 to 16.0), and incomplete aneurysm obliteration (OR 2.6; 95% CI 1.0 to 6.6). Higher impact journals were more likely to publish papers on endovascular techniques, particularly flow-diverter stents. Conclusions None of the methods is unequivocally superior. Considering its inefficiency, stent-assisted coiling should be undertaken with caution. A time-delimited systematic review is needed to establish the most accurate treatment for ruptured BBAs.
The Art and Science of Mental Health Nursing: A Textbook of Principles

Contributors Preface Foreword PART 1: FOUNDATIONS The origins and expression of psychological distress Mental health promotion Mental health nursing: origins and orientations The policy and service context for mental health nursing Rehabilitation and recovery Law and ethics of mental health nursing PART 2: INTERVENTIONS Assessment and care planning Assessing and managing risk The therapeutic milieu Psychosocial interventions Pharmacological interventions and electro-convulsive therapy Complementary and alternative therapies PART 3: APPLICATIONS The person with a perceptual disorder The person with a mood disorder The person with an anxiety disorder The person with an eating disorder The person who misuses drugs or alcohol Mental health problems in childhood and adolescence The older person with dementia or other mental health problems The person who uses forensic mental health services The person with a personality disorder PART 4: CORE PROCEDURES Engaging clients in their care and treatment Problems, goals and care planning Behavioural techniques Cognitive techniques Medication management to concordance Therapeutic management of aggression and violence Therapeutic management of attempted suicide and self-harm PART 5: FUTURE DIRECTIONS Functional teams and whole systems Reflections Index.
Finding MIMO

Availability of multiple antennas enables increased capacity or increased resilience for modern radios. This advantage depends on the deployment of the antennas at the sender and receiver. But there is a performance gap between most simulated results and the actual performance obtained in practice. This is due to the rank of the channel obtained in deployments, which depends on local propagation conditions, and on the placement of the senders and receivers. Using implementation on top of USRP platform, and mobile antennas, we show that it is possible to find 'good' antenna positions within a search space of a few carrier wavelengths. This opens the possibility for adaptive methods in antenna position and coding/modulation techniques to feed back to each other to reduce the gap between theoretical and practical MIMO performance.
Audio-Visual Temporal Recalibration Can be Constrained by Content Cues Regardless of Spatial Overlap

It has now been well established that the point of subjective synchrony for audio and visual events can be shifted following exposure to asynchronous audio-visual presentations, an effect often referred to as temporal recalibration. Recently it was further demonstrated that it is possible to concurrently maintain two such recalibrated estimates of audio-visual temporal synchrony. However, it remains unclear precisely what defines a given audio-visual pair such that it is possible to maintain a temporal relationship distinct from other pairs. It has been suggested that spatial separation of the different audio-visual pairs is necessary to achieve multiple distinct audio-visual synchrony estimates. Here we investigated if this is necessarily true. Specifically, we examined whether it is possible to obtain two distinct temporal recalibrations for stimuli that differed only in featural content. Using both complex (audio visual speech; see Experiment 1) and simple stimuli (high and low pitch audio matched with either vertically or horizontally oriented Gabors; see Experiment 2) we found concurrent, and opposite, recalibrations despite there being no spatial difference in presentation location at any point throughout the experiment. This result supports the notion that the content of an audio-visual pair alone can be used to constrain distinct audio-visual synchrony estimates regardless of spatial overlap.
Dynamic Coded Cooperation Using Multiple Turbo Codes in Wireless Relay Networks

In this paper, we propose a dynamic coded cooperation using multiple turbo codes in wireless relay networks where both relay and destination are equipped with a sensing device and the relay is assumed to determine autonomously if it should cooperate or not. We first elucidate the fact that the puncturing patterns of the multiple turbo codes determine the achievable diversity order by deriving their pairwise error probabilities (PEP). This is followed by a derivation of a simple power detector at the destination that can estimate a precise duration of cooperation phase upon decoding information. Furthermore, we investigate the impact of process delay due to cognitive processing at the relay, which results in the loss of diversity gain, and propose a simple null padding as its countermeasure. Computer simulations show that our proposed approach is able to achieve a full diversity and the frame error rate (FER) performance superior to that of conventional cooperative communications that make use of turbo coding structure even in practical scenarios.
Spelling Error Trends in Urdu

Today the most accurate error correction techniques are statistical. But for low resourced languages like Urdu, where training error corpora are not available, statistical techniques are out of the question. Rule based techniques that exploit spelling error trends provide a useful alternative. The study of error patterns in a language is an essential prerequisite for designing such techniques. This paper presents two studies of spelling error trends in Urdu. The results show that alongside the already known spelling error trends common to all languages, Urdu also exhibits some language specific error patterns. The most important among them are space related errors and shape similarity based errors. They form a dominating portion of the total spelling mistakes in Urdu.