Miracle-dz/UniFAR-FTUs huggingface dataset:
The hypothalamic-pituitary axis in genetically obese (ob/ob) mice: response to luteinizing hormone-releasing hormone.

The obese (genotype ob/ob) mouse is known to have a genetically acquired form of hypogonadotropic hypogonadism. Because the animal has multiple abnormalities of endocrine systems, including impaired growth, obesity, and temperature regulation, it has been assumed by many to have a hypothalamic defect. In an attempt to separate hypothalamic from pituitary dysfunction, an acute LHRH response test was administered to obese and lean littermates. In both lean and ob/ob adult male animals, a bolus of LHRH increased serum LH concentration, but the concentrations attained were 2-fold greater in the lean animals. To determine if the defect in the obese animals was the result of chronic understimulation of the pituitary gland due to LHRH deficiency, LHRH was administered three times daily for 20 days, after which the acute LHRH test was repeated. After 20 days of LHRH treatment, the obese animals remained less responsive than the lean mice to a bolus of LHRH. Surprisingly, in both lean and ob/ob animals, chronic LH.
Oligomeric Interaction of Hepatitis C Virus NS5B Is Critical for Catalytic Activity of RNA-dependent RNA Polymerase*

HCV NS5B is an RNA-dependent RNA polymerase (RdRP), a central catalytic enzyme for HCV replication, which has the “palm and fingers” substructure. We recently identified five novel residues critical for RdRP activity (Qin, W., Yamashita, T., Shirota, Y., Lin, Y., Wei, W., and Murakami, S. (2001)Hepatology 33, 728–737). Among them, GLU-18 and His-502, far from the catalytic center, may be involved in conformational change(s) for RdRP activity as addressed in some palm and fingers enzymes. We examined the possibility that NS5B is oligomerized, and we could detect the interaction between two different tagged NS5B proteins in vitro and transiently expressed in mammalian cells. By scanning 27 clustered and then point alanine substitutionsin vivo and in vitro, Glu-18 and His-502 were found to be critical for the homomeric interaction in vivoand in vitro, strongly suggesting a close relationship between the oligomerization and RdRP activity of NS5B. All mutants with substitutions at these two residues failed to bind wild type NS5B, however E18H interacted with H502E in vitro and in vivo. Interestingly, the NS5B protein with E18H or H502E did not exhibit RdRP activity, but a mixture of the two mutant proteins did. These results clearly indicate that two residues of HCV NS5B are critical for the oligomerization that is prerequisite to RdRP activity.
EFECTO DE DIFERENTES SUSTRATOS EN CRECIMIENTO Y RENDIMIENTO DE TOMATE (Lycopersicum esculentum Mill) BAJO CONDICIONES DE INVERNADERO

El tomate ( Lycopersicum esculentum Mill .) a nivel mundial es la segunda hortaliza de mayor importancia. En Mexico, el cultivo cobra relevancia economica y social generando divisas y empleos, los sistemas de produccion de esta hortaliza se han ido diversificando con el fin de incrementar el rendimiento, incorporando tecnologias novedosas como cubiertas plasticas, riego por goteo e hidroponia. Uno de los principales factores que determinan el exito del cultivo es el sustrato, pues constituyen el medio en que se desarrollaran las raices las cuales tienen gran influencia en el crecimiento y desarrollo. En este trabajo se evaluo durante el ciclo agricola 2008–2009 el efecto de los sustratos: Aserrin de pino, composta de estiercol de ovino, tierra agricola y tezontle rojo; en el crecimiento, y rendimiento del tomate. El diseno experimental que se utilizo fue bloques completamente al azar, con cuatro repeticiones y se evaluaron diez tratamientos producto de la combinacion de los sustratos a un volumen de 1:1, cada unidad experimental estuvo constituida por cuatro plantas, las variables estudiadas fueron sometidas a un analisis de varianza (ANOVA) mediante el paquete estadistico Statistical Package for the Social Sciences (SPSS). El genotipo que se utilizo fue Sun 7705. Se detectaron diferencias significativas entre sustratos, la mezcla aserrin-composta afecto en mayor respuesta las variables altura 4.61 m, grosor del tallo 2.1 cm, frutos de mayor peso 107.8 g, y rendimiento por planta de 4 kg y 25 kg/m -2 . Sin embargo, el numero de flores y de racimos fue mayor en el sustrato aserrin, por lo que la mezcla aserrin-composta puede ser una opcion viable para producir tomate en invernadero.
Mobile Music Listening – an aesthetic and aestheticizing practice

How are we to conceptualize the experiences of mobile music listeners as they move through the city listening to a private soundtrack? The phenomenon has been described as an aestheticization of urban space (Bull, 2002, 2005, 2007; Hosokawa 1984 a.o.), and this perspective seems to focus on the listeners’ wish to alter and manipulate the impression of the surroundings. This article, however, explores and re-thinks how the terms aestheticization and aesthetic experi ence may be used to describe the specific relationship between the listener and the surround ings, which is established through the act of listening. To support this focus and claim I refer amongst others to Morten Kyndrup’s thoughts on the aesthetic relation (Kyndrup, 2008a) and Mikel Dufrenne’s theory on the aesthetic object as a perceived object (Dufrenne, 1973, 2001).
Implementing public-key infrastructure for sensor networks

We present a critical evaluation of the first known implementation of elliptic curve cryptography over F2p for sensor networks based on the 8-bit, 7.3828-MHz MICA2 mote. We offer, along the way, a primer for those interested in the field of cryptography for sensor networks. We discuss, in particular, the decisions underlying our design and alternatives thereto. And we elaborate on the methodologies underlying our evaluation. Through instrumentation of UC Berkeley's TinySec module, we argue that, although symmetric cryptography has been tractable in this domain for some time, there has remained a need, unfulfilled until recently, for an efficient, secure mechanism for distribution of secret keys among nodes. Although public-key infrastructure has been thought impractical, we show, through analysis of our original implementation for TinyOS of point multiplication on elliptic curves, that public-key infrastructure is indeed viable for TinySec keys' distribution, even on the MICA2. We demonstrate that public keys can be generated within 34 seconds and that shared secrets can be distributed among nodes in a sensor network within the same time, using just over 1 kilobyte of SRAM and 34 kilobytes of ROM. We demonstrate that communication costs are minimal, with only 2 packets required for transmission of a public key among nodes. We make available all of our source code for other researchers to download and use. And we discuss recent results based on our work that corroborate and improve upon our conclusions.
HDAC6 Brain Mapping with [18F]Bavarostat Enabled by a Ru-Mediated Deoxyfluorination

Histone deacetylase 6 (HDAC6) function and dysregulation have been implicated in the etiology of certain cancers and more recently in central nervous system (CNS) disorders including Rett syndrome, Alzheimer’s and Parkinson’s diseases, and major depressive disorder. HDAC6-selective inhibitors have therapeutic potential, but in the CNS drug space the development of highly brain penetrant HDAC inhibitors has been a persistent challenge. Moreover, no tool exists to directly characterize HDAC6 and its related biology in the living human brain. Here, we report a highly brain penetrant HDAC6 inhibitor, Bavarostat, that exhibits excellent HDAC6 selectivity (>80-fold over all other Zn-containing HDAC paralogues), modulates tubulin acetylation selectively over histone acetylation, and has excellent brain penetrance. We further demonstrate that Bavarostat can be radiolabeled with 18F by deoxyfluorination through in situ formation of a ruthenium π-complex of the corresponding phenol precursor: the only method currently suitable for synthesis of [18F]Bavarostat. Finally, by using [18F]Bavarostat in a series of rodent and nonhuman primate imaging experiments, we demonstrate its utility for mapping HDAC6 in the living brain, which sets the stage for first-in-human neurochemical imaging of this important target.
The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 68031[W]

Changing of principal σ factor in RNA polymerase holoenzyme to a group 2 σ factor redirects transcription when cyanobacteria acclimate to suboptimal environmental conditions. The group 2 sigma factor SigB was found to be important for the growth of the cyanobacterium Synechocystis sp. PCC 6803 in high-salt (0.7 m NaCl) stress but not in mild heat stress at 43°C although the expression of the sigB gene was similarly highly, but only transiently up-regulated at both conditions. The SigB factor was found to regulate many salt acclimation processes. The amount of glucosylglycerol-phosphate synthase, a key enzyme in the production of the compatible solute glucosylglycerol, was lower in the inactivation strain ΔsigB than in the control strain. Addition of the compatible solute trehalose almost completely restored the growth of the ΔsigB strain at 0.7 m NaCl. High-salt conditions lowered the chlorophyll and phycobilin contents of the cells while protective carotenoid pigments, especially zeaxanthin and myxoxanthophyll, were up-regulated in the control strain. These carotenoids were up-regulated in the ΔsigCDE strain (SigB is the only functional group 2 σ factor) and down-regulated in the ΔsigB strain under standard conditions. In addition, the HspA heat shock protein was less abundant and more abundant in the ΔsigB and ΔsigCDE strains, respectively, than in the control strain in high-salt conditions. Some cellular responses are common to heat and salt stresses, but pretreatment with mild heat did not protect cells against salt shock although protection against heat shock was evident.
Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Mutations in the forkhead transcription factor gene FOXL2 are involved in ovarian failure, which occurs in human BPES syndrome. This syndrome presents a sexually dimorphic expression, specific to the ovary in several vertebrates. We cloned the open reading frame of chicken FOXL2 (cFoxL2) and studied cFoxL2 expression in developing gonads and during adulthood to examine the role of FOXL2 in ovarian differentiation and function in birds. The spatial and temporal dynamics of cFoxL2 and aromatase expression were analyzed in parallel by using real‐time quantitative reverse transcriptase‐polymerase chain reaction and immunohistochemistry in attempt to investigate the possible role of cFoxL2 in the regulation of aromatase. The expression patterns of cFoxL2 and aromatase transcripts were highly correlated during the sex‐differentiation period (4.7–12.7 days of incubation). Aromatase and cFoxL2 proteins were colocalized in the medullar part of female gonads on embryonic day 14. Fourteen days after hatching, cFoxL2 protein was mainly detected in granulosa cells of developing follicles. In adult ovary follicular envelopes, apart from granulosa cells, cFoxL2 transcript and protein were detected at lower levels in theca cells where aromatase was present. A high level of cFoxL2 transcription was also observed in maturing and ovulated oocytes. Our results confirm that FoxL2 is an early regulator of ovarian development in birds and may be involved in aromatase transcription regulation. Developmental Dynamics 859–870, 2004. © 2004 Wiley‐Liss, Inc.
Prenatal testosterone improves the spatial learning and memory by protein synthesis in different lobes of the brain in the male and female rat

The high density of the steroid hormone receptors in the structures of temporal lobe involved in learning and memory, such as the hippocampus, perirhinal cortex, entorhinal cortex and amigdaloid complex, shows that there must be a direct relationship between gonadal hormones and organizational effects of steroid hormones in those structures during development of the nervous system. The present study was undertaken in order to investigate the effect of testosterone administration during the third week of gestation on the spatial memory formation of the offspring rats and the level of soluble proteins in the temporal lobe and frontal lobe of brain, as evidence of important organizational effects of androgens during prenatal development in brain sexual dimorphism. Animals have received testosterone undecanoate on days 14, 15, 16 and 19, 20, 21 of gestation. Learning and memory tests were started 100 days after the testosterone treatment. At the end of the experiments, the temporal and frontal lobes of brain were removed for assessing the level of soluble proteins. Testosterone treatment significantly improved spontaneous alternations percentage of male offspring in Y-maze task comparative with female offspring and reference memory in radial 8 arm-maze task (decreasing in number of reference memory errors in both male and female offspring groups), suggesting effects of both short and long-term memory. Also, testosterone significantly increased the brain soluble protein level of treated female rats in 14–16 prenatal days compared with the control group as well as the brain soluble protein level of treated male rats. These results suggest that steroid hormones play an important role in the spatial learning and memory formation by means of protein synthesis in different lobes of the brain.
Species cross-reactivity of rheumatoid factors and implications for immunoassays

Abstract Rheumatoid factors (RFs) are antibodies recognizing other antibodies usually by binding to the Fc part, while heterophilic antibodies (HAbs) are antibodies reacting with immunoglobulins (Igs) from other species. In particular, RFs have been found to cause false positive results in sandwich immunoassays. In this work, we analyzed RF-positive and RF-negative sera for content of cytokines and for heterophilic reactions by enzyme-linked immunosorbent assay and bead-based sandwich immunoassays. All sera, including those with RFs, contained insignificant amounts of cytokines and chemokines, but RF-positive sera showed large false positive values for several cytokines when analyzed by fluorescent bead-based multiplex immunoassays. This non-specific binding could be minimized by reagents designed to block HAbs, i.e. by selected animal IgGs. Furthermore, sera positive for RFs reacted with several animal IgGs, when these were immobilized on beads or coated on the polystyrene surface in enzyme-linked immunosorbent assays. This reaction could be inhibited by human IgG and by agents designed to inhibit heterophilic reactions (i.e. mixtures of IgGs from different species). In conclusion, RFs and HAbs represent an identical/overlapping set of antibodies, causing false positive reactions in sandwich and other immunoassays. Such assays must be conducted in the presence of appropriate blocking agents, e.g. HBR+, and must be carefully controlled.
The Efficacy of Spirometry as a Screening Tool in Detection of Air Flow Obstruction

Background/Objectives: In developing countries, spirometry has not been considered a part of routine medical check-up. The purpose of the study was to establish the usefulness of spirometry as a primary screening tool in detecting air flow obstruction (AFO) during routine medical check-up (RMC). Methods: This was a hospital based, retrospective, non-randomized case series study of 3696 participants, who presented to hospital for routine medical check-up. All subjects were assisted at the Pulmonary Medicine Department, from January 2003 till December 2008 who, having met other inclusion criteria, underwent spirometry. Data were analyzed using proportion, group means, standard deviations and Pearson Chi Square test. Results: The overall yield from spirometry in detecting AFO was 211 patients (5.7%); 174 males (6.1%) and 37 females (4.4%) (P=0.158, Pearson Chi Square test). Greater age at presentation and BMI correlated significantly with AFO in the target group (P=<0.001; P=<0.005) respectively. Dyspnoea was the most frequent symptomatology observed in those diagnosed with AFO. Conclusion: These results suggest that spirometry during RMC for all persons can detect a significant number of patients with AFO particularly among the middle and older age groups with a low BMI.
“Tension” in South Asian Women: Developing a Measure of Common Mental Disorder Using Participatory Methods

Background: Although common mental disorder (CMD) is highly prevalent among South Asian immigrant women, they rarely seek mental treatment. This may be owing in part to the lack of conceptual synchrony between medical models of mental disorder and the social models of distress common in South Asian communities. Furthermore, common mental health screening and diagnostic measures may not adequately capture distress in this group. Community-based participatory research (CBPR) is ideally suited to help address measurement issues in CMD as well as to develop culturally appropriate treatment models. Objectives: To use participatory methods to identify an appropriate, culturally specific mental health syndrome and develop an instrument to measure this syndrome. Methods: We formed a partnership between researchers, clinicians, and community members. The partnership selected a culturally specific model of emotional distress/illness, “tension,” as a focus for further study. Partners developed a scale to measure Tension and tested the new scale on 162 Bangladeshi immigrant women living in the Bronx. Results: The 24-item “Tension Scale” had high internal consistency (α = 0.83). On bivariate analysis, the scale significantly correlated in the expected direction with depressed as measured by the Patient Health Questionnaire (PHQ-2), age, education, self-rated health, having seen a physician in the past year, and other variables. Conclusions: Using participatory techniques, we created a new measure designed to assess CMD in an isolated immigrant group. The new measure shows excellent psychometric properties and will be helpful in the implementation of a community-based, culturally synchronous intervention for depression. We describe a useful strategy for the rapid development and field testing of culturally appropriate measures of mental distress and disorder.
Phase I and Pharmacokinetic Study of the (6-Maleimidocaproyl)Hydrazone Derivative of Doxorubicin

Purpose: The (6-maleimidocaproyl)hydrazone derivative of doxorubicin (DOXO-EMCH) is an albumin-binding prodrug of doxorubicin with acid-sensitive properties that shows superior antitumor efficacy in murine tumor models and a favorable toxicity profile in mice, rats, and dogs compared with doxorubicin. The purpose of the phase I study was to characterize the toxicity profile of DOXO-EMCH, establish a recommended dose for phase II studies, and assess potential anticancer activity. Experimental Design: A starting dose of 20 mg/m2 doxorubicin equivalents was chosen. Forty-one patients with advanced cancer disease were treated with an i.v. infusion of DOXO-EMCH once every 3 weeks at a dose level of 20 to 340 mg/m2 doxorubicin equivalents. Results: Treatment with DOXO-EMCH was well tolerated up to 200 mg/m2 without manifestation of drug-related side effects. Myelosuppression (grade 1-2) and mucositis (grade 1-2) were the predominant adverse effects at dose levels of 260 mg/m2 and myelosuppression (grade 1-3) as well as mucositis (grade 1-3) were dose limiting at 340 mg/m2. No cardiac toxicity was observed. Of 30 of 41 evaluable patients, 12 patients (40%) had progressive disease, 15 patients (57%) had stable disease, and 3 patients (10%) had a partial remission. Conclusions: DOXO-EMCH showed a good safety profile and was able to induce tumor regressions in tumor types known to be anthracycline-sensitive tumors, such as breast cancer, small cell lung cancer, and sarcoma. The recommended doxorubicin equivalent dose for phase II studies is 260 mg/m2.
Empirical analysis of the correlation between amount-of-reuse metrics in the C programming language

In recent years, there have been several proposals for amount-ofreuse metrics. However, it is not often clear if these metrics measure anything different than already used software metrics, or what the relations between these metrics really are. Without this, the true added value provided by each metric is unknown. In this paper, an analysis of the existing amount-of-reuse metrics is presented and a portion of them are selected on the basis of their industrial applicability. These metrics are then applied to a collection of public domain software products, to obtain an understanding of the level of correlation that exists between them and other well known software metrics such as cyclomatic complexity, volume and lines of code. Through this process we find that a subset of the selected amount-of-reuse metrics provide a relatively independent view of the analyzed code.
Communication, Mediation, and the Expectations of Data: Data Valences Across Health and Wellness Communities

Communication technologies increasingly mediate data exchanges rather than human communication. We propose the term data valences to describe the differences in expectations that people have for data across different social settings. Building on two years of interviews, observations, and participation in the communities of technology designers, clinicians, advocates, and users for emerging mobile data in formal health care and consumer wellness, we observed the tensions among these groups in their varying expectations for data. This article identifies six data valences (self-evidence, actionability, connection, transparency, “truthiness,” and discovery) and demonstrates how they are mediated and how they are distinct across different social domains. Data valences give researchers a tool for examining the discourses around, practices with, and challenges for data as they are mediated across social settings.
MEMS-based AC voltage reference

An ac root-mean-square (RMS) voltage reference based on a microelectromechanical system (MEMS) component is presented. The device stability is investigated in various experiments. A time stability at a level of a few /spl mu/V/V in 24 h was measured using an accelerometer MEMS component at an operating frequency of 100 kHz.
Genetic susceptibility to the respiratory effects of air pollution

There is large variation between individuals in their response to air pollutants. This review summarises the existing evidence that genetic factors influence the mechanisms of lung injury caused by air pollutants. Genetic association studies have compared the adverse effects of air pollutants between subjects with specific genotypes in biologically relevant genes. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. Inflammatory gene polymorphisms (TNF) influence the lung function response to ozone, and the effect of different levels of ozone on the development of asthma. Polymorphisms in oxidative stress genes (GSTM1, GSTP1) alter the response to combined exposure to ragweed pollen and diesel exhaust particles. Importantly, polymorphisms in an oxidative stress gene (GSTM1) have predicted patients with asthma who benefit from antioxidant supplementation in Mexico City, which has chronically high ozone exposure. Genetic linkage studies of families have not been feasible for studying the effects of air pollution in humans, but some progress has been made with pedigrees of specially bred mice, in identifying chromosomal regions linked to effects of ozone or particles. A high priority now, in addition to avoiding exposure in the most susceptible people, is to clearly identify the most effective and safe chemopreventive agents for individuals who are genetically susceptible to the adverse effects of air pollution (eg, antioxidants to be taken during high ozone levels).