Miracle-dz/UniFAR-FTUs huggingface dataset:
Applicability of papain solutions in immunohematology

ABSTRACT Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
Electrification of aerosol particles in supersonic atomizers

Factors limiting charge-to-mass ratio achievable for induction-charged aerosol produced in supersonic atomizers are discussed. Experimental investigations performed using supersonic atomizers fitted with de Laval nozzle and concentric induction electrode have demonstrated moderately low electrification levels obtained in case of elevated flow rate of liquid being atomized. It was shown that low charging degree is associated with occurrence of shielding effect. A simplified model of conducting liquid charging process was worked out for the case of atomized liquid cone shielded by an "umbrella" of droplets. Manifestation of the shielding phenomenon and regularities arising from the assumed model has been confirmed experimentally.
Multiple sequence alignment using anytime A*

Alignment of multiple DNA or protein sequences is a central problem in computational biology. To create an alignment, gaps are inserted into sequences to shift characters to matching positions and a scoring function is used to rank the biological plausibility of alignments. Multiple sequence alignments are used to identify homologies among different species that reveal evolutionary history from a common ancestor. They are also used to discover genetic causes of certain diseases and to predict protein structure, which has significant importance in the design of drugs. The multiple sequence alignment problem can be formalized as a shortest-path problem through a d-dimensional lattice, where d is the number of sequences to be aligned (Gusfield 1997). Dynamic programming is the traditional approach to constructing optimal alignments. Improved performance has recently been achieved using A*. However, the multiple alignment problem presents a difficulty for the classic A* algorithm. Its branching factor of 2 d − 1 is so large that the size of the open list dramatically exceeds the number of nodes A* must expand to find an optimal solution. Two solutions to this problem have been proposed in the literature. Yoshizumi et al. (2000) describe an extension of A*, called A* with Partial Expansion (PEA*). Instead of generating all successors of a node when it is expanded, PEA* inserts only the most promising successors into the open list. The “partially expanded” node is re-inserted into the open list with a revised f-cost equal to the least f-cost
Formation mechanism of low-density lipoprotein gel induced by NaCl.

Salted eggs, which are a traditional Chinese egg product, are favored by Chinese consumers and have become very popular in other Asian countries due to their unique features such as "fresh, fine, tender, loose, gritty and oily texture. " In order to illuminate the forming process of salted egg, the gelation behavior and mechanism of low-density lipoprotein (LDL) induced by NaCl were investigated using marinated model outside the eggshell. Results showed that as the salting proceeded, the moisture content exhibited a decreasing trend. The NaCl content, oil exudation, hardness, and surface hydrophobicity showed constantly increasing trends. In the early stages of salting, the size of the LDL particles, soluble protein content, and T21 increased, whereas T21 (with D2O), T22, and the free sulfhydryl content declined. In the later stages of salting, LDL formed a multiple composite aggregate gel structure with filamentous apoproteins and non-spherical lipid particles intertwined with each other. The soluble protein content and T23 (without D2O) decreased, whereas T21 (with D2O), T22 and the free sulfhydryl content increased. Fourier transform infrared spectroscopy revealed that the fresh LDL mainly consisted of α-helix and β-sheet structures. After the gel becomes hardened, the LDL secondary structure was changed remarkably, characterized by the decrease of α-helix elements and increase of β-sheet elements. The results suggested that the oil exudation of salted LDL gels was mainly due to LDL destruction and the release of components (apoproteins, phospholipids, and neutral lipids) facilitated by increased interactions between apoproteins and lipids.
Association between use of lung-protective ventilation with lower tidal volumes and clinical outcomes among patients without acute respiratory distress syndrome: a meta-analysis.

CONTEXT Lung-protective mechanical ventilation with the use of lower tidal volumes has been found to improve outcomes of patients with acute respiratory distress syndrome (ARDS). It has been suggested that use of lower tidal volumes also benefits patients who do not have ARDS. OBJECTIVE To determine whether use of lower tidal volumes is associated with improved outcomes of patients receiving ventilation who do not have ARDS. DATA SOURCES MEDLINE, CINAHL, Web of Science, and Cochrane Central Register of Controlled Trials up to August 2012. STUDY SELECTION Eligible studies evaluated use of lower vs higher tidal volumes in patients without ARDS at onset of mechanical ventilation and reported lung injury development, overall mortality, pulmonary infection, atelectasis, and biochemical alterations. DATA EXTRACTION Three reviewers extracted data on study characteristics, methods, and outcomes. Disagreement was resolved by consensus. DATA SYNTHESIS Twenty articles (2822 participants) were included. Meta-analysis using a fixed-effects model showed a decrease in lung injury development (risk ratio [RR], 0.33; 95% CI, 0.23 to 0.47; I2, 0%; number needed to treat [NNT], 11), and mortality (RR, 0.64; 95% CI, 0.46 to 0.89; I2, 0%; NNT, 23) in patients receiving ventilation with lower tidal volumes. The results of lung injury development were similar when stratified by the type of study (randomized vs nonrandomized) and were significant only in randomized trials for pulmonary infection and only in nonrandomized trials for mortality. Meta-analysis using a random-effects model showed, in protective ventilation groups, a lower incidence of pulmonary infection (RR, 0.45; 95% CI, 0.22 to 0.92; I2, 32%; NNT, 26), lower mean (SD) hospital length of stay (6.91 [2.36] vs 8.87 [2.93] days, respectively; standardized mean difference [SMD], 0.51; 95% CI, 0.20 to 0.82; I2, 75%), higher mean (SD) PaCO2 levels (41.05 [3.79] vs 37.90 [4.19] mm Hg, respectively; SMD, -0.51; 95% CI, -0.70 to -0.32; I2, 54%), and lower mean (SD) pH values (7.37 [0.03] vs 7.40 [0.04], respectively; SMD, 1.16; 95% CI, 0.31 to 2.02; I2, 96%) but similar mean (SD) ratios of PaO2 to fraction of inspired oxygen (304.40 [65.7] vs 312.97 [68.13], respectively; SMD, 0.11; 95% CI, -0.06 to 0.27; I2, 60%). Tidal volume gradients between the 2 groups did not influence significantly the final results. CONCLUSIONS Among patients without ARDS, protective ventilation with lower tidal volumes was associated with better clinical outcomes. Some of the limitations of the meta-analysis were the mixed setting of mechanical ventilation (intensive care unit or operating room) and the duration of mechanical ventilation.
Prepartum fatty acid supplementation in sheep IV. Effect of calcium salts with eicosapentaenoic acid and docosahexaenoic acid in the maternal and finishing diet on lamb liver and adipose tissue during the lamb finishing period.

The objective of this study was to evaluate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation to ewes during late gestation on finishing lamb liver and adipose tissue fatty acid (FA) profile and gene expression. Lambs born from ewes supplemented with Ca salts of EPA + DHA, or palm FA distillate (PFAD) high in palmitic and oleic acid at 0.39% DM during the last 50 d of gestation were used. Lambs were weaned at 61 d of age and adapted to a high concentrate diet for 1.5 mo. After adaptation, 74 lambs (28 pens) were blocked by sex and BW and used in a 2x2 factorial arrangement of treatments using the factors of dam supplementation (DS) and lamb supplementation (LS) of Ca salts of EPA + DHA or PFAD at 1.48% DM. Lambs were slaughtered after 42 d and liver and adipose tissue collected for fatty acid and gene expression analysis. Liver concentrations of EPA and DHA were greater (P < 0.01) with LS of EPA + DHA vs. PFAD during the finishing period. In adipose tissue, a lamb x dam interaction was observed for EPA (P = 0.02) and DHA (P = 0.04); LS of EPA + DHA increased EPA and DHA, but the increase was greatest in lambs born from ewes supplemented with PFAD. No lamb x dam treatment interactions were observed for gene expression in liver tissue (P > 0.10). Hepatic mRNA abundance of hormonesensitivelipase (HSL; P = 0.01) was greater in lambs born from EPA + DHA ewes vs. lambs from PFAD ewes. mRNA expression of stearoyl-CoAdesaturase (P < 0.01), fattyacidsynthase (P = 0.01), Δ5-desaturase (P < 0.01) and Δ6-desaturase (P < 0.01) were decreased in liver of EPA + DHA lambs. A significant lamb x dam diet interaction was observed for elongationofverylongchainfattyacid2inadiposetissue (P = 0.01); lambs supplemented with the same FA as their dams had lower expression. Expression of HSL tended (P = 0.08) to be decreased in adipose of EPA + DHA lambs born from EPA + DHA ewes. The changes in mRNA expression suggest that lipogenesis decreased, and lipolysis increased in lamb liver with EPA + DHA vs. PFAD supplementation during the finishing period. In adipose tissue, changes suggest that lipogenesis decreased in lambs born from EPA + DHA supplemented dams and supplemented with EPA + DHA during the finishing period. Additionally, these results suggest an interaction between supplementation of FA to dams during late gestation on lamb response of adipose tissue, but not liver, to FA supplementation during the finishing period.
Use of Statin Medications Following Diagnosis in Relation to Survival among Women with Ovarian Cancer

Background: It has been suggested that the likelihood of survival among women with ovarian cancer could be increased by postdiagnosis statin use. This study examines the potential association between postdiagnosis statin use and cancer-specific mortality among women with ovarian cancer. Methods: This cohort study used SEER-Medicare data on women ≥66 years of age diagnosed with ovarian cancer during 2007 to 2012 who were enrolled in Medicare parts A, B, and D during the year after diagnosis. Statin use was defined as two or more fills for a statin during the year after diagnosis. Ovarian cancer–specific death was assessed starting 1 year after diagnosis. Marginal structural Cox models were used, adjusting for the inverse probability of treatment weighting and censoring weighting. Treatment weights and censoring weights were calculated using logistic regression models with a priori–defined covariates. Results: Among 2,195 women with ovarian cancer, 489 (22%) used statins within 1 year after their diagnosis. Over a mean follow-up of 2.2 years, 796 (36%) women died from ovarian cancer. The adjusted HR for ovarian cancer mortality comparing statin users to nonusers was 0.74 (95% confidence interval, 0.61–0.91). Conclusions: Findings from this and prior work suggest statin use following a diagnosis with ovarian cancer is associated with a lower risk of cancer death. Impact: Because, in most women, statin administration has limited side effects, a randomized trial of statins among patients with ovarian cancer may be warranted.
cDNA Cloning and Characterization of a Cotton Peptide Methionine Sulfoxide Reductase (cMsrA)

A full-length cDNA clone with high homology with Brassica napus peptide methionine sulfoxide reductase (PMSR) gene (BnPMSR), which could reverse oxidation of methionine residues into MetSO in protein, was cloned by suppression subtraction hybridization and cDNA library screening from Gossypium barbadense. This gene (named as cMsrA; Accession number: AY224208) had a total length of 1059 bp with an open reading frame of 765 bp, and encoded a predicted polypeptide of 255 amino acids with a molecular weight of 28.4 kDa. The cMsrA protein shared 68.6, 66.3 and 65.8% identity with those PMSR proteins isolated from B. napus, A. thaliana and L. sativa respectively. Expression patterns revealed that cMsrA was enriched later and weaker in resistant variety 7124 (Gossypium barbadense) than sensitive variety Ejing-1 (G. hirsutum) under salt treatment and pathogens attack. These results indicated that cMsrA played an important role in protecting the cells against oxidative damage during the pathogens and salt stress.
Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler chickens reared under heat stress.

The aim of this study was to examine the effect of a dietary synbiotic supplement on the cecal microflora, antioxidant status, and immune response of broiler chickens under heat stress (HS). A total of 360 one-day-old male Ross 708 broiler chicks were randomly distributed among 3 dietary treatments containing a synbiotic (PoultryStar consists of Bifidobacterium animalis, Enterococcus faecium, Lactobacillus reuteri, Pediococcus acidilactici, and fructooligosaccharides) at 0 (control), 0.5 (0.5X), and 1.0 (1.0X) g/kg. Each treatment contained 8 replicates of 15 birds each housed in floor pens. Heat stimulation was at 32°C for 9 h daily from day 15 to 42. Heat stress-induced changes of cecal bacteria were detected using bacteria-specific agars, and spleen protein concentration and mRNA expression of interleukins and antioxidants were examined using ELISA and real-time PCR, respectively. Under the HS condition, synbiotic fed broilers regardless of dose had lower cecal enumerations of Escherichia coli and coliforms, and a lower heterophil/lymphocyte (H/L) ratio (P < 0.05) compared to controls. 1.0X group also had higher cecal enumerations of Bifidobacterium spp. and Lactobacillus spp. , spleen glutathione peroxidase (GPx), and plasma nuclear factor erythroid 2-related factor 2 (Nrf-2), and a lower H/L ratio compared to both control and 0.5X groups (P < 0.05). However, there were no treatment effects on the levels of Enterococcus spp. , the circulating monocytes, eosinophils, and basophils, Toll like receptor-4 (TLR-4), interleukin-6 (IL-6), interlukin-10 (IL-10), and their mRNA expression, as well as plasma Kelch-like ECH-associated protein 1 (Keap-1) (P > 0.05). These results suggest that the synbiotic could inhibit the negative effects of HS on broiler health through the reduction of cecal pathogens, regulation of stress reactions, and improvement of antioxidant status.
Experimental anxiety and the reinforcing effects of ethanol in rats.

In order to examine the relationship between anxiety and reinforcing effects of alcohol, drug-naive male Wistar rats weighing 250-300 g were classified as "anxious" and "non-anxious" in the elevated plusmaze test. A conditioned place preference test was then used to investigate the reinforcing effects of ethanol (EtOH) on these animals. On 2 alternate days, groups of "anxious", "non-anxious" and "normal" rats received intraperitoneal (i.p.) injections of EtOH (0.5, 1.0 or 1.5 g/kg) immediately before a 15-min confinement to the white compartment. On the 2 intervening days the same rats received i.p. injections of saline before confinement to the opposite compartment. On day 5, a 15-min free-choice test was carried out with no injections. Rats classified as "anxious" showed a significant, though not dose-dependent preference for all doses of ethanol compared to saline-treated animals. These data demonstrate that rats regarded as "anxious" are more sensitive to the reinforcing effects of EtOH than "non-anxious" and "normal" Wistar rats and emphasize the relevance of the basal levels of anxiety of rats when trying to detect the reinforcing effects of EtOH.
Microbial and Functional Profile of the Ceca from Laying Hens Affected by Feeding Prebiotics, Probiotics, and Synbiotics

Diet has an essential influence in the establishment of the cecum microbial communities in poultry, so its supplementation with safe additives, such as probiotics, prebiotics, and synbiotics might improve animal health and performance. This study showed the ceca microbiome modulations of laying hens, after feeding with dry whey powder as prebiotics, Pediococcus acidilactici as probiotics, and the combination of both as synbiotics. A clear grouping of the samples induced per diet was observed (p < 0.05). Operational taxonomic units (OTUs) identified as Olsenella spp. , and Lactobacillus crispatus increased their abundance in prebiotic and synbiotic treatments. A core of the main functions was shared between all metagenomes (45.5%), although the genes encoding for the metabolism of butanoate, propanoate, inositol phosphate, and galactose were more abundant in the prebiotic diet. The results indicated that dietary induced-changes in microbial composition did not imply a disturbance in the principal biological roles, while the specific functions were affected.
Effect of Post-Harvest LED and UV Light Irradiation on the Accumulation of Flavonoids and Limonoids in the Segments of Newhall Navel Oranges (Citrus sinensis Osbeck)

To investigate the effect of post-harvest light irradiation on the accumulation of flavonoids and limonoids, harvested Newhall navel oranges were continuously exposed to light-emitting diode (LED) and ultraviolet (UV) light irradiation for 6 days, and the composition and content of flavonoids and limonoids in the segments were determined using UPLC-qTOF-MS at 0, 6, and 15 days after harvest. In total, six polymethoxylated flavonoids (PMFs), five flavone-O/C-glycosides, seven flavanone-O-glycosides, and three limonoids were identified in the segments. The accumulation of these components was altered by light irradiation. Red and blue light resulted in higher levels of PMFs during exposure periods. The accumulation of PMFs was also significantly induced after white light, UVB and UVC irradiation were removed. Red and UVC irradiation induced the accumulation of flavone and flavanone glycosides throughout the entire experimental period. Single light induced limonoid accumulation during exposure periods, but limonoid levels decreased significantly when irradiation was removed. Principal component analysis showed a clear correlation between PMFs and white light, between flavonoid glycosides and red light and UVC, and between limonoids and UVC. These results suggest that the accumulation of flavonoids and limonoids in citrus is regulated by light irradiation. White light, red light and UVC irradiation might be a good potential method for improving the nutrition and flavor quality of post-harvest citrus.
Reproductive fitness of Drosophila is maximised by optimal developmental temperature

ABSTRACT Whether the character of developmental plasticity is adaptive or non-adaptive has often been a matter of controversy. Although thermal developmental plasticity has been studied in Drosophila for several traits, it is not entirely clear how it affects reproductive fitness. We, therefore, investigated how developmental temperature affects reproductive performance (early fecundity and egg-to-adult viability) of wild-caught Drosophila melanogaster. We tested competing hypotheses on the character of developmental thermal plasticity using a full-factorial design with three developmental and adulthood temperatures within the natural thermal range of this species. To account for potential intraspecific differences, we examined flies from tropical (India) and temperate (Slovakia) climate zones. Our results show that flies from both populations raised at an intermediate developmental temperature (25°C) have comparable or higher early fecundity and fertility at all tested adulthood temperatures, while lower (17°C) or higher developmental temperatures (29°C) did not entail any advantage under the tested thermal regimes. Importantly, the superior thermal performance of flies raised at 25°C is apparent even after taking two traits positively associated with reproductive output into account: body size and ovariole number. Thus, in D. melanogaster, development at a given temperature does not necessarily provide any advantage in this thermal environment in terms of reproductive fitness. Our findings strongly support the optimal developmental temperature hypothesis, which states that in different thermal environments, the highest fitness is achieved when an organism is raised at its optimal developmental temperature. Summary: Developmental acclimation does not necessarily enhance reproductive fitness in Drosophila melanogaster; instead, the highest fitness is achieved by development at an optimal temperature.
Sexually Dimorphic Shaping of Interneuron Dendrites Involves the Hunchback Transcription Factor

Sexual dimorphism of the brain has been well characterized anatomically in Drosophila melanogaster at the single neuron level, yet little is known about the molecular mechanism whereby cellular sex differences are generated except that the neural sex determination gene fruitless (fru) plays a key role. The fru-expressing mAL interneuron cluster is sexually dimorphic in three aspects: the number of cells composing the cluster is 5 in females and 30 in males; the ipsilateral neurite is absent in females and present in males; the contralateral neurite forms Y-shaped branches in the subesophageal ganglion in females while it ends with a simple horsetail-like structure in males. By screens in the compound eye for modifiers of roughness induced by fru+ overexpression, we identified a loss-of-function allele of hunchback (hb) to be a suppressor of this phenotype. Hb was expressed in most of the fru-expressing neurons in the pupal and adult stages. Knocking down hb in mAL MARCM (Mosaic Analysis with a Repressible Cell Marker) clones in the male brain resulted in partial demasculinization of the branching pattern of the contralateral neurites without affecting the cell number and the ipsilateral neurite formation. The present results suggest that Hb is essential for male-typical shaping of the contralateral neurites by Fru.
Progress in medical information management. Systematized nomenclature of medicine (SNOMED).

THE LAST 50 years have witnessed an explosion in the scientific advances available to the medical practitioner in his evaluation and treatment of the patient. The arrival of computer technology has helped to augment the physician's recall of myriad pieces of information to help him evaluate the data for diagnosis and treatment. Inherent in this process is the need for medicine to develop a standardized language for the classification of disease into general groups, as well as detailed schemas for the naming of signs, symptoms, diseases, and procedures. To be useful, the system needs to be completely integrated. We review the historical development of classifications for statistical purposes and the conceptual evolution of nomenclatures and discuss the new nomenclature-classification system called the Systematized Nomenclature of Medicine (SNOMED) . 1 Present and future requirements of the management of medical information will be presented, including SNOMED applications. PART I STATISTICAL MANAGEMENT: CLASSIFICATION VS