Miracle-dz/UniFAR-FTUs huggingface dataset:
Monitoring extracellular pH, oxygen, and dopamine during reward delivery in the striatum of primates

Dopamine projections that extend from the ventral tegmental area to the striatum have been implicated in the biological basis for behaviors associated with reward and addiction. Until recently, it has been difficult to evaluate the complex balance of energy utilization and neural activity in the striatum. Many techniques such as electrophysiology, functional magnetic resonance imaging (fMRI), and fast-scan cyclic voltammetry have been employed to monitor these neurochemical and neurophysiological changes. In this brain region, physiological responses to cues and rewards cause local, transient pH changes. Oxygen and pH are coupled in the brain through a complex system of blood flow and metabolism as a result of transient neural activity. Indeed, this balance is at the heart of imaging studies such as fMRI. To this end, we measured pH and O2 changes with fast-scan cyclic voltammetry in the striatum as indices of changes in metabolism and blood flow in vivo in three Macaca mulatta monkeys during reward-based behaviors. Specifically, the animals were presented with Pavlovian conditioned cues that predicted different probabilities of liquid reward. They also received free reward without predictive cues. The primary detected change consisted of pH shifts in the striatal extracellular environment following the reward predicting cues or the free reward. We observed three types of cue responses that consisted of purely basic pH shifts, basic pH shifts followed by acidic pH shifts, and purely acidic pH shifts. These responses increased with reward probability, but were not significantly different from each other. The pH changes were accompanied by increases in extracellular O2. The changes in pH and extracellular O2 are consistent with current theories of metabolism and blood flow. However, they were of sufficient magnitude that they masked dopamine changes in the majority of cases. The findings suggest a role of these chemical responses in neuronal reward processing.
IP based security architecture of virtual network in cloud computing system

Advantages of a cloud computing sharing IT resources can also be vulnerable from the security perspective. Because users could be allocated in the same virtual network with adversary users, the traffic generated by adversary users causes a decline in the performance of the virtual machine of others users. This leads to the problem of erroneous billing. Proposed architecture minimizes the influence of malicious traffic using the classification of traffic and encryption based on an IP routing table. The analysis shows additional resources required to the proposed architecture in a cloud computing system through performance evaluation using real dataset gathered from a company. Further we suggest effective method to apply proposed architecture in a cloud computing system.
Clinical aplications of Trioxolane derivatives

The aqueous extracts of three medicinal plants, Carissa edulis (Forssk.) Vahl (Apocynaceae), Prunus africana (Hook.f.) Kalkm (Rosaceae) and Melia azedarach L. (Meliaceae) have shown significant reduction in the replication of human cytomegalovirus (HCMV) in human embryonic lung (HEL) fibroblasts cells in vitro. Using the plaque inhibition assay for the determination of anti-viral activity, the HEL fibroblast cells cultured in 24 well plates were infected with 1 x 102 PFU 91S HCMV and treated with various concentrations of the extracts. The plaques formed were counted after 7 days incubation at 37°C in 5% CO2 and the percent plaques inhibited were calculated against infected untreated control. The effective concentrations inhibiting plaque formation by 50% (EC50) was found between 40 to 80 μg/ml for all the extracts. The cell cytotoxic concentrations (CC50) for each of the three extracts, by the trypan blue exclusion test, gave a safe therapeutic index. These results have demonstrated the potential anti-viral activities of the extracts of the three medicinal plants at non-cytotoxic concentrations African Journal of Health Sciences Vol. 15 (1&2) 2008: pp. 1-5
Blockon: a block based buildable remote controller

There are many consumer electronics placed on our home. When we control these consumer electronics remotely, we are often forced to switch between remote controllers. Further, the increased functions of electronics also increase a number of buttons of remote controllers. These complexities make it difficult for users to control consumer electronics. To simplify interaction between remote controllers and the electronics, we propose a user buildable remote controller device, called Blockon. The Blockon enables a user to build his own controller easily by placing button blocks on the board as building LEGO blocks. The Blockon not only supports placing buttons freely but also makes it possible to define macro commands by stacking button blocks. In this paper, we also present a prototype of the Blockon for technical verification along with our future plan.
Inhibitory Potential of Antifungal Drugs on ATP-Binding Cassette Transporters P-Glycoprotein, MRP1 to MRP5, BCRP, and BSEP

ABSTRACT Inhibition of ABC transporters is a common mechanism underlying drug-drug interactions (DDIs). We determined the inhibitory potential of antifungal drugs currently used for invasive fungal infections on ABC transporters P-glycoprotein (P-gp), MRP1 to MRP5, BCRP, and BSEP in vitro. Membrane vesicles isolated from transporter-overexpressing HEK 293 cells were used to investigate the inhibitory potential of antifungal drugs (250 μM) on transport of model substrates. Concentration-inhibition curves were determined if transport inhibition was >60%. Fifty percent inhibitory concentrations (IC50s) for P-gp and BCRP were both 2 μM for itraconazole, 5 and 12 μM for hydroxyitraconazole, 3 and 6 μM for posaconazole, and 3 and 11 μM for isavuconazole, respectively. BSEP was strongly inhibited by itraconazole and hydroxyitraconazole (3 and 17 μM, respectively). Fluconazole and voriconazole did not inhibit any transport for >60%. Micafungin uniquely inhibited all transporters, with strong inhibition of MRP4 (4 μM). Anidulafungin and caspofungin showed strong inhibition of BCRP (7 and 6 μM, respectively). Amphotericin B only weakly inhibited BCRP-mediated transport (127 μM). Despite their wide range of DDIs, azole antifungals exhibit selective inhibition on efflux transporters. Although echinocandins display low potential for clinically relevant DDIs, they demonstrate potent in vitro inhibitory activity. This suggests that inhibition of ABC transporters plays a crucial role in the inexplicable (non-cytochrome P450-mediated) DDIs with antifungal drugs.
Somatosensory temporal discrimination in patients with primary focal dystonia

Purposes: To determine whether somatosensory temporal discrimination will reliably detect subclinical sensory impairment in patients with various forms of primary focal dystonia. Methods: The somatosensory temporal discrimination threshold (STDT) was tested in 82 outpatients affected by cranial, cervical, laryngeal and hand dystonia. Results were compared with those for 61 healthy subjects and 26 patients with hemifacial spasm, a non-dystonic disorder. STDT was tested by delivering paired stimuli starting with an interstimulus interval of 0 ms followed by a progressively increasing interstimulus interval. Results: STDT was abnormal in all the different forms of primary focal dystonias in all three body regions (eye, hand and neck), regardless of the distribution and severity of motor symptoms. Receiver operating characteristic curve analysis calculated in the three body regions yielded high diagnostic sensitivity and specificity for STDT abnormalities. Conclusions: These results provide definitive evidence that STDT abnormalities are a generalised feature of patients with primary focal dystonias and are a valid tool for screening subclinical sensory abnormalities.
Convergent Evidence among Content-Specific Versions of Working Memory Tests

The evidence pertaining to inferences derived from working memory test scores does not appear as developed as the evidence documenting the relation between working memory and performance. Gaps in the literature include documenting the relations among working memory tests of similar and different item content. The authors proposed that data from these tests would fit a correlated two-factor model best where the latent factors are based on item content. A total of 201 undergraduates completed four versions of a working memory measure. Confirmatory factor analytic results supported our hypothesis. These data suggest that some working memory measures are not interchangeable.
Patterns, Factors, and Pathways Contributing to Nutritional Risk Among Rural African American Elders

In the United States, the increasing life expectancy and burgeoning older population have raised concerns about the adequate provision of health care for chronic medical conditions. One fundamental factor in the prevention and management of medical conditions is optimal dietary intake, which is notably lacking among older adults. Most researchers investigating factors associated with suboptimal diets limit their studies to developing nutritional risk profiles, and many conclude by advocating patient education. Using telephone surveys and in-depth interviews, this article identifies particularly vulnerable elders and explores the contextual and experiential pathways that place individuals at nutritional risk. Surveys of African American and white elders indicate that rural African American elders are disproportionately at risk of inadequate nutrition. In-depth interviews revealed four pathways to nutritional risk, including transitions in the social and physical significance of food, inaccessibility of res.
Path-planning modules for Autonomous Vehicles: Current status and challenges

A detailed survey of the available literature on path planning of Autonomous Ground Vehicle (AGV) is conducted, including the overview of single-robot control architectures, different path-planning approaches, analyses of current sensor systems and different velocity estimation techniques. In order to achieve the full autonomous operation of a mobile robot, path or motion planning, i.e., the planning of a collision-free path from a start to goal position through a collection of obstacles, is the fundamental task in the field of autonomous control systems. As AGVs are used in a wide variety of applications to perform autonomous tasks, organising their intelligence plays a key role in successfully programming a robot for a particular application and applying the right control architecture makes the autonomous control problem easier to solve. For autonomous vehicles, sensors play an important role in acquiring different attributes of the working environment and, by extracting meaningful information from these data, the autonomous system can acquire knowledge about its environment. Moreover, different velocity estimation techniques are reviewed in the context of dealing with the dynamic obstacles. A brief review of the available literature on path-planning approaches and techniques is provided.
Macitentan for the treatment of pulmonary arterial hypertension

Macitentan is a novel dual endothelin receptor antagonist recently approved for the treatment of symptomatic pulmonary arterial hypertension (PAH). Compared to other endothelin receptor antagonists, in vitro and in vivo studies have demonstrated that macitentan has improved tissue targeting, a longer duration of action and an improved safety profile. Macitentan is available as a once daily oral medication and has been well tolerated in clinical trials. The recently published Study with an Endothelin Receptor Antagonist in PAH to Improve cliNical Outcomes (SERAPHIN), which was the first event-driven trial ever done in PAH, also demonstrated the benefit of macitentan on reducing the likelihood of a composite endpoint of morbidity and mortality events without a significant difference in mortality.
Surface-emitting laser-its birth and generation of new optoelectronics field

The surface-emitting laser (SEL) is considered one of the most important devices for optical interconnects and LANs, enabling ultra parallel information transmission in lightwave and computer systems. We introduce its history, fabrication technology, and discuss the advantages. Then, we review the progress of the surface emitting laser and the vertical-cavity surface-emitting laser (VCSEL), covering the spectral band from infrared to ultraviolet by featuring its physics, materials, fabrication technology, and performances, such as threshold, output powers, polarizations, line-width, modulation, reliability, and so on.
Tobacco or Healthy Children: The Two Cannot Co-Exist

Tobacco exposure increases mortality and morbidity of the fetus, the child, the adolescent, and their children in turn. Nearly half the children in the world are exposed. Smoking is not merely personal choice or personal responsibility; those subtle phrases undermine those who have no choice in the matter. Tobacco control must take a multi-pronged attack. Smoking cessation by adults in childbearing years must take center stage of these efforts, because it is the only way to ensure a smoke-free environment for children. Smoke-free parents provide a role model for smoke-free young people, and erode the image of smoking as a desirable adult behavior to emulate. Pediatricians and pediatric pulmonologists have a key role to play here. This goal will reduce morbidity and mortality among adults and children. Legislation regarding taxation, environments, tobacco constituents, product placement and display, packaging, and media education are all key to this core goal. Smoke-free policy must be protected from attack based on trade agreements. Research is needed into more effective ways to attract and help people give up smoking, and into educating and re-deploying tobacco industry workers in emerging and developed countries.
The Facilitation of Thinking Skills in Preschool Children using Computer Presented Activities

Summary Thirty six four‐year‐old children were given the Raven's Coloured Progressive Matrices and were then randomly divided within sexes into two groups. A treatment group worked through sixteen computer presented problem‐solving activities while the second was a controlled group and did not do the activities. All the children were then re‐tested on Raven's Matrices. The results showed a significantly greater improvement between the pre‐ and post‐test for the treatment group than for the control group. The results are discussed in terms of the development of thinking skills.
Propofol‐induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice

Thousands of infants and children are undergoing anesthesia around the world every day. But impacts of anesthetics on the developing neural system remain unclear yet. Previous evidence showed that anesthesia might affect the developing neural system. Thus, early‐life anesthesia becomes a critical issue in clinical pediatric practice. Hence, propofol, a short‐acting and widely applied intravenous anesthetic, has been gaining focus upon neonatal anesthesia.
Living with and looking at landscape

Abstract Landscape is experienced in countless ways by all human beings, both individually and as members of communities, nations and humanity as a whole. Concern for rural locales as the loci of social, economic and domestic existence has, in recent centuries, often been seen in accord, but more usually in conflict, with attachment to the scenic qualities of landscape couched in aesthetic terms. Celebrated in art and in history, landscapes connote stability and security, but living with them is regarded as a virtue, looking at them condemned as shallow scenic appreciation. The stress between these two sets of values is exacerbated by the decline of rural economies throughout the developed world, the abandonment of agricultural landscapes and the loss of traditional countryside ties. Shifting landscape attachments reflect the timing, extent and current pace of rural depopulation. Whether despite or owing to their increasing remoteness from everyday life, landscapes are heavily freighted with moral and symbolic worth as ecological paradigms and as rightful common inheritances, while spurned as scenically frivolous.
THE EUROPEAN WAY TO HEALTH TECHNOLOGY ASSESSMENT

Objectives: The EUR-ASSESS project was undertaken by a large number of members of European health technology assessment (HTA) agencies and programs with the aim of improving coordination through developments in several areas of HTA. At the conclusion of the project, an independent survey was undertaken to reassess the importance of the original objectives of EUR-ASSESS and determine the impact of the project on the way HTA activities are organized and carried out in Europe. Methods: A postal questionnaire survey and a series of more in-depth personal interviews. Results: EUR-ASSESS was generally considered successful in achieving its aims, particularly in creating an informal network of people and organizations, improving the understanding of the work of others, improving the ability to focus the objectives of HTA, facilitating the sharing of experience and mutual learning opportunities, exchanging ideas on research agendas, and developing a common language. However, significant differences emerged in the expectations of different actors in European HTA, and in their perceptions of the impact of the project. These reflect the wide diversity of approaches existing in Europe. Conclusions: Health technology assessment in Europe is changing its nature from a set of national or local initiatives with relatively limited impact to a truly European movement with strong links and coordination between its individual components. Important lessons can be learned from the experience of EUR-ASSESS, particularly in the light of an increasing commitment of the European Commission to play a role in the development of technology assessment.
Histone arginine demethylase JMJD6 is linked to stress granule assembly through demethylation of the stress granule–nucleating protein G3BP1

Stress granules (SG) are membrane-less organelles that are condensates of stalled translation initiation complexes and mRNAs. SG formation is a cytoprotective response to environmental stress and results from protein interactions involving regions of low amino acid complexity and poorly defined post-translational modifications of SG components. Many RNA-binding proteins are methylated, and we previously demonstrated that the potent SG–nucleating protein G3BP1 is methylated by protein arginine methyltransferase 1 and 5 (PRMT1 and PRMT5). G3BP1 methylation represses SG formation and is reversible. Here we functionally link JMJD6 (Jumonji C domain-containing protein 6) to G3BP1 demethylation. Our findings reveal that JMJD6 is a novel SG component that interacts with G3BP1 complexes, and its expression reduces G3BP1 monomethylation and asymmetric dimethylation at three Arg residues. Knockdown of JMJD6 repressed SG formation and G3BP1 demethylation, but SG formation and G3BP1 demethylation were rescued with catalytically active but not mutant JMJD6. These results suggest that JMJD6 functions directly or indirectly as an arginine demethylase of G3BP1 that promotes SG formation.
GnRH antagonist in older IVF patients. Retrieval rates and clinical outcome.

OBJECTIVE To determine if the use of a midcycle GnRH antagonist provides better clinical outcomes and lower cancellation rates in in vitro fertilization (IVF). STUDY DESIGN We examined all patients older than 40 years undergoing IVF-embryo transfer cycles between January 1999 and December 2000. Prior to June 2000, controlled ovarian stimulation in women > or = 40 years was performed with follicle stimulating hormone (FSH)/human menopausal gonadotropin (hMG) only and no GnRH agonist or antagonist (group I). After June 2000, following the release of Ganirelix in the U.S., all women > or = 40 years were stimulated with FSH/hMG + Ganirelix (group II). Outcomes of IVF cycles prior to Ganirelix were compared to results after its introduction. RESULTS Cancellation rates were significantly lower in group II (16%) as compared to group I (67%) (P < .05). In patients with oocytes retrieved, group II had a significantly higher number of recovered oocytes (7.7 +/- 0.8 vs. 5.3 +/- 0.7, P < .05). However, the number of embryos transferred, cumulative embryo scores, implantation rates and ongoing pregnancy rates did not differ significantly between groups. CONCLUSION Although our results are preliminary, the addition of GnRH antagonist avoids ovarian suppression at the start of controlled ovarian hyperstimulation and prevents the premature LH surge at midcycle. Thus, more patients attempting IVF undergo oocyte retrieval, although clinical outcomes may not necessarily be improved.
Colouration in crab spiders: substrate choice and prey attraction

SUMMARY Australian crab spiders Thomisus spectabilis ambush pollinating insects, such as honeybees (Apis mellifera) on flowers, and can change their body colour between yellow and white. It is traditionally assumed that the spiders change their colour to match the flower colour, thus rendering them cryptic to insect prey. Here, we test this assumption combining state-of-the-art knowledge of bee vision and behavioural experiments. In the field, yellow spiders are only found on yellow daisies (Chrysanthemum frutescens), whereas white spiders are found on yellow and white daisies. These field patterns were confirmed in the laboratory. When given the choice between white and yellow daisies, yellow spiders preferred yellow daisies, whereas white spiders showed only a slight but non-significant preference for white flowers. Thus, T. spectabilis select background colours according to their own body colour. When viewed from a distance, bees use an achromatic signal produced by their green receptors for target detection. Through this visual channel, white spiders on white flowers, and yellow spiders on yellow flowers are virtually undetectable. From a closer distance of a few centimetres, when bees evaluate colour contrast, the combination of spider colour against different flower backgrounds affected the response of honeybees, but not in ways predicted by a classical crypsis/conspicuousness interpretation. Yellow spiders on yellow flowers are not perfectly matched when interpreted through the colour vision of a honeybee. Nevertheless, honeybees showed indifference to the presence of a spider, equally landing on vacant or spider-occupied flowers. Likewise, white spiders are poorly hidden on white flowers, as white spiders reflect ultraviolet light strongly, while white flowers do not. Surprisingly, bees are attracted to this contrast, and significantly more honeybees preferred white flowers occupied by white spiders. White spiders on yellow flowers produce the highest colour contrast and bees again preferred spider-occupied flowers. Yellow spiders on white flowers were the only pairing where bees rejected spider-occupied flowers, especially in cases where the contrast between the two was relatively strong. Thus, T. spectabilis select flower colours adaptively in a way that deceives honeybees, or at least does not deter them.