Miracle-dz/UniFAR-FTUs huggingface dataset:
Workplace impact on employees: A Lifelines Corona Research Initiative on the return to work

A large proportion of the global workforce migrated home during the COVID-19 pandemic and subsequent lockdowns. It remains unclear what the exact differences between home workers and non-home workers were, especially during the pandemic when a return to work was imminent. How were building, workplace, and related facilities associated with workers’ perceptions and health? What are the lessons to be learned? Lifelines Corona Research Initiative was used to compare employees’ workplaces and related concerns, facilities, work quality, and health in a complete case analysis (N = 12,776) when return to work was imminent. Mann-Whitney U, logistic regression, and Wilcoxon matched-pairs were used for analyses. Notwithstanding small differences, the results show that home workers had less favourable scores for concerns about and facilities of on-site buildings and workplaces upon return to work, but better scores for work quality and health than non-home workers. However, additional analyses also suggest that building, workplace, and related facilities may have had the capacity to positively influence employees’ affective responses and work quality, but not always their health.
Evaluation of Tick Abundance and Pyrethroid Resistance Via Determination of Glutathione-S-Transferases Activity

Abstract Controlling the number of ticks as carriers of infectious diseases is very important. The process is sometimes compromised by activating the protective mechanisms of the tick itself. Glutathione-S-transferases activity (GSTs) was the subject of our investigation of tick abundance after pyrethroid treatment. We determined GSTs activity in ticks collected from six locations in Belgrade before and after pyrethroid treatment and correlated it with the number of ticks in the locations. The results showed that tick abundance correlated with GSTs activity. On the other hand, treatment efficiency was location-dependent, being similar in each particular location in both April (spring) and October (autumn). Our results suggest that GSTs activity reflects the influence of both present local allelochemicals from different environmental seasonal vegetation and applied pyrethroid. We can conclude that by evaluating GSTs activity in ticks from particular locations as well as during the treatment with acaricides tick removal practice could be improved.
“A breath of fresh air”: mindfulness training for early-career mental health social workers in Israel during COVID-19

ABSTRACT The present study examined the effectiveness of participation in a mindfulness group for Israeli early career social workers during the COVID-19 period. Eleven participants conducting fieldwork in a mental health rehabilitation center were interviewed. The participants described a variety of benefits from their mindfulness training, in three main areas: (1) Personal: applying mindfulness in their daily life; (2) Family: improved work-life balance and family relationships; and (3) Professional: applying mindfulness in their work with mental health clients. Mental support and self-care tools provided to early-career social workers were perceived by the participants as helping them overcome uncertainty, fatigue, and overwork.
Elemental abundances of the supernova remnant G292.0+1.8: evidence for a massive progenitor

We present a comprehensive nonequilibrium ionization (NEI) analysis of X-ray spectral data from the Einstein Observatory and EXOSAT for the supernova remnant G292.0+1.8. The spectra are well described by a single-temperature, single-timescale NEI model with kT = 1.64(sub -0.19)(sup +0.29) keV and n(sub e)t = (5.55(sub -1.12)(sup +1.2) x 10(exp 10)s/cu cm, which establishes that this remnant is indeed young and in the ionizing phase of evolution of its X-ray spectrum. We determine the abundances of the elements O, Ne, Mg, Si, S, Ar, and Fe and examine their variation over the allowed range of column density, kT, and n(sub e)t. Numerical calculations of the nucleosynthesis expected for a 25 solar mass progenitor agree best with the fitted abundances; in fact the minimum rms percent difference between this model and the derived abundances is only 15%. From the fitted emission measure and a simple geometric model of the remnant we estimate the mass of X-ray-emitting plasma to be 9.3(sub -6.2)(sup +1.19) solar mass, for an assumed distance of 4.8 +/- 1.6 kpc. Additional errors on this mass estimate, from clumping of the ejecta, for example, may be substantial. No evidence was found for a difference in the thermodynamic state of the plasma as a function of elemental composition based on analysis of the individual ionization timescales of the various species. In this sense then, G292.0+1.8 resembles the remnant Cas A (another product of a massive star supernova), while it is different from the remnants of SN 1572 (Tycho) and SN 1006, both of which are believed to be from Type Ia supernovae.
The transcription factor c-Jun inhibits RBM39 to reprogram pre-mRNA splicing during genotoxic stress

Abstract Genotoxic agents, that are used in cancer therapy, elicit the reprogramming of the transcriptome of cancer cells. These changes reflect the cellular response to stress and underlie some of the mechanisms leading to drug resistance. Here, we profiled genome-wide changes in pre-mRNA splicing induced by cisplatin in breast cancer cells. Among the set of cisplatin-induced alternative splicing events we focused on COASY, a gene encoding a mitochondrial enzyme involved in coenzyme A biosynthesis. Treatment with cisplatin induces the production of a short isoform of COASY lacking exons 4 and 5, whose depletion impedes mitochondrial function and decreases sensitivity to cisplatin. We identified RBM39 as a major effector of the cisplatin-induced effect on COASY splicing. RBM39 also controls a genome-wide set of alternative splicing events partially overlapping with the cisplatin-mediated ones. Unexpectedly, inactivation of RBM39 in response to cisplatin involves its interaction with the AP-1 family transcription factor c-Jun that prevents RBM39 binding to pre-mRNA. Our findings therefore uncover a novel cisplatin-induced interaction between a splicing regulator and a transcription factor that has a global impact on alternative splicing and contributes to drug resistance.
The holistic approach to the CHRNA7 gene, hsa-miR-3158-5p, and 15q13.3 hotspot CNVs in migraineurs

Migraine is a neurological disease characterized by severe headache attacks. Combinations of different genetic variations such as copy number variation (CNV) in a gene and microRNA (miRNA) expression can provide a holistic approach to the disease as a pathophysiological, diagnostic, and therapeutic target. CNVs, the Cholinergic Receptor Nicotinic Alpha 7 Subunit (CHRNA7) gene, and expression of gene-targeting miRNAs (hsa-miR-548e-5p and hsa-miR-3158-5p) in migraineurs (n = 102; with aura, n = 43; without aura, n = 59) and non-migraines (n = 120) aged 15–60 years, comparative, case–control study was conducted. Genetic markers were compared with biochemical parameters (BMI, WBC, Urea, GFR, ESR, CRP, HBG). All analyzes were performed by quantitative Real-Time PCR (q-PCR) and fold change was calculated with the 2−ΔΔCT method. The diagnostic power of the CHRNA7 gene, CNV, and miRNAs were analyzed with the receiver operating curve (ROC). CHRNA7 gene and hsa-miR-3158-5p are down-regulated in migraineurs and the gene is controlled by this miRNA via CNVs (p < .05). Both deletion and duplication were detected in patients with migraine for CVN numbers (p = .05). The number of CNV deletions was higher than duplications. When CHRNA7-CNV-hsa-miR-3158-5p was modeled together in the ROC analysis, the area under the curve (AUC) was 0.805, and the diagnostic power was “good”. In migraineurs, the CHRNA7 gene can be controlled by hsa-miR-3158-5p via CNVs to modulate the mechanism of pain. These three genetic markers have diagnostic potential and may be used in antimigraine treatments.
Does perceived post-traumatic growth during the COVID-19 pandemic reflect actual positive changes?

ABSTRACT Background and Objectives People commonly report positive changes following stressful experiences (perceived posttraumatic growth; PPTG), yet whether PPTG validly reflects positive changes remains unestablished. Design and Methods We tested the extent to which COVID-19 pandemic-related PPTG relates to positive changes in corresponding psychosocial resources in a national US sample participating in a five wave study (T1-T5), focusing here on T2-T5: ns = 712–860. We examined correlations between resource change (both latent and observed difference scores) and PPTG at each occasion and conducted structural equation models to separate occasion-specific and stable (traitlike) PPTG variance. We related changes in resources to occasion-specific and stable PPTG components. Results Associations between change scores and occasion-specific PPTG were sparse, providing limited evidence of PPTG validity. Associations between change scores and stable PPTG tended to be positive and stronger than associations for occasion-specific PPTG. Discussion Perceptions of growth were largely unrelated to experienced positive changes and thus appear to be largely illusory. However, a personality-like tendency to believe one grows from stressful experiences relates more strongly to actual resource growth. These results suggest that people are not accurate reporters of positive changes they experience and that interventions aimed at promoting post-traumatic growth may be premature.
Robust non-fragile stabilisation and H∞ control for uncertain discrete-time stochastic systems with Markovian jump parameters and time-varying delay

In this paper, the robust non-fragile stabilisation and H∞ control problem is investigated for a class of uncertain discrete-time stochastic systems with Markovian jumping parameters and time-varying delay. The parameter uncertainties are supposed to be time-varying as well as norm-bounded. The aim of the robust non-fragile stabilisation problem is to design a non-fragile state feedback controller which guarantees the robust stability of the closed-loop system for all admissible uncertainties. At the same time, in addition to the robust stability requirement, a prescribed H∞ performance level is required to be achieved for the robust H∞ control problem. By Lyapunov stability theory, delay-segment-dependent conditions for the solvability of these problems are formulated in terms of linear matrix inequality technique. Finally, numerical examples are shown to demonstrate the usefulness and applicability of the proposed design method.
The bulge/halo luminosity of M33 and its globular cluster population

The method of surface photometry is used to constrain the bulge luminosity of M 33. Analysis of blue and 12 μm images places a conservative upper limit of M v = −15 on the bulge luminosity of M 33. Although there is a clear power-law component in the surface brightness profile of M 33 interior to R = 0.5 kpc, it represents less than 2% of the total luminosity. More stringent limits come by considering the relatively blue colors observed in the inner regions of M 33. It is unclear if this power law component represents a bulge or a more extended halo component. By itself, the presence of a puny bulge/halo in a disk galaxy is not very interesting
Motion artifact reduction for magnetic resonance imaging with deep learning and k-space analysis

Motion artifacts deteriorate the quality of magnetic resonance (MR) images. This study proposes a new method to detect phase-encoding (PE) lines corrupted by motion and remove motion artifacts in MR images. 67 cases containing 8710 slices of axial T2-weighted images from the IXI public dataset were split into three datasets, i.e., training (50 cases/6500 slices), validation (5/650), and test (12/1560) sets. First, motion-corrupted k-spaces and images were simulated using a pseudo-random sampling order and random motion tracks. A convolutional neural network (CNN) model was trained to filter the motion-corrupted images. Then, the k-space of the filtered image was compared with the motion-corrupted k-space line-by-line, to detect the PE lines affected by motion. Finally, the unaffected PE lines were used to reconstruct the final image using compressed sensing (CS). For the simulated images with 35%, 40%, 45%, and 50% unaffected PE lines, the mean peak signal-to-noise ratio (PSNRs) of resulting images (mean±standard deviation) were 36.129±3.678, 38.646±3.526, 40.426±3.223, and 41.510±3.167, respectively, and the mean structural similarity (SSIMs) were 0.950±0.046, 0.964±0.035, 0.975±0.025, and 0.979±0.023, respectively. For images with more than 35% PE lines unaffected by motion, images reconstructed with proposed algorithm exhibited better quality than those images reconstructed with CS using 35% under-sampled data (PSNR 37.678±3.261, SSIM 0.964±0.028). It was proved that deep learning and k-space analysis can detect the k-space PE lines affected by motion and CS can be used to reconstruct images from unaffected data, effectively alleviating the motion artifacts.
Tetrandrine inhibits migration and invasion of BGC‐823 and MKN‐45 cells by regulating PI3K/AKT/mTOR signaling pathway

Tetrandrine (Tet), a traditional Chinese herbal medicine extract, exhibits anti‐cancer effect on many types of cancer. Nonetheless, the action mechanism of Tet in gastric cancer (GC) is still largely unclear. In the current study, proliferation, invasion, and migration of the BGC‐823 and MKN‐45 cells were effectively suppressed by Tet treatment in a dose‐dependent manner. Moreover, Tet suppressed expression of the proliferation‐associated protein PCNA, the interstitial cell phenotype N‐cadherin, and the extracellular matrix‐associated MMP‐2 and MMP‐9 in BGC‐823 and MKN‐45 cells in a dose‐dependent manner. PI3K/AKT/mTOR, a cancer promoting signaling, was inactivated by Tet in a dose‐dependent manner in BGC‐823 and MKN‐45 cells. Furthermore, our results demonstrated that the inhibition of Tet to PCNA, N‐cadherin, MMP‐2, and MMP‐9 expression was partly rescuedby AKT inhibitor or mTOR inhibitor. In animal experiments, tumor growth was inhibited by Tet administration in a dose‐dependent manner. In conclusion, the current data indicated that Tet had a critical effect on inhibiting BGC‐823 and MKN‐45 cells proliferation, migration, invasion, and tumor growth via regulating PI3K/AKT/mTOR signaling pathway, suggesting that Tet might be a potential treatment for GC.
Combined Single‐Cell and Spatial Transcriptomics Reveal the Metabolic Evolvement of Breast Cancer during Early Dissemination

Breast cancer is now the most frequently diagnosed malignancy, and metastasis remains the leading cause of death in breast cancer. However, little is known about the dynamic changes during the evolvement of dissemination. In this study, 65 968 cells from four patients with breast cancer and paired metastatic axillary lymph nodes are profiled using single‐cell RNA sequencing (scRNA‐seq) and spatial transcriptomics. A disseminated cancer cell cluster with high levels of oxidative phosphorylation (OXPHOS), including the upregulation of cytochrome C oxidase subunit 6C and dehydrogenase/reductase 2, is identified. The transition between glycolysis and OXPHOS when dissemination initiates is noticed. Furthermore, this distinct cell cluster is distributed along the tumor's leading edge. The findings here are verified in three different cohorts of breast cancer patients and an external scRNA‐seq dataset, which includes eight patients with breast cancer and paired metastatic axillary lymph nodes. This work describes the dynamic metabolic evolvement of early disseminated breast cancer and reveals a switch between glycolysis and OXPHOS in breast cancer cells as the early event during lymph node metastasis.
Plasma membrane topography governs the 3D dynamic localization of IgM B cell antigen receptor clusters

B lymphocytes recognize bacterial or viral antigens via different classes of the B cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces, and are thought to perform sensory functions in screening antigen‐bearing surfaces. Here, we have used lattice light‐sheet microscopy in combination with tailored custom‐built 4D image analysis to study the cell‐surface topography of B cells of the Ramos Burkitt's Lymphoma line and the spatiotemporal organization of the IgM‐BCR. Ramos B‐cell surfaces were found to form dynamic networks of elevated ridges bridging individual microvilli. A fraction of membrane‐localized IgM‐BCR was found in clusters, which were mainly associated with the ridges and the microvilli. The dynamic ridge‐network organization and the IgM‐BCR cluster mobility were linked, and both were controlled by Arp2/3 complex activity. Our results suggest that dynamic topographical features of the cell surface govern the localization and transport of IgM‐BCR clusters to facilitate antigen screening by B cells.
Landmark-dependent Navigation Strategy Declines across the Human Life-Span: Evidence from Over 37,000 Participants

Abstract Humans show a remarkable capacity to navigate various environments using different navigation strategies, and we know that strategy changes across the life span. However, this observation has been based on studies of small sample sizes. To this end, we used a mobile app–based video game (Sea Hero Quest) to test virtual navigation strategies and memory performance within a distinct radial arm maze level in over 37,000 participants. Players were presented with six pathways (three open and three closed) and were required to navigate to the three open pathways to collect a target. Next, all six pathways were made available and the player was required to visit the pathways that were previously unavailable. Both reference memory and working memory errors were calculated. Crucially, at the end of the level, the player was asked a multiple-choice question about how they found the targets (i.e., a counting-dependent strategy vs. a landmark-dependent strategy). As predicted from previous laboratory studies, we found the use of landmarks declined linearly with age. Those using landmark-based strategies also performed better on reference memory than those using a counting-based strategy. These results extend previous observations in the laboratory showing a decreased use of landmark-dependent strategies with age.
Well-Separated Clusters and Optimal Fuzzy Partitions

Abstract Two separation indices are considered for partitions P = {X1, …, Xk} of a finite data set X in a general inner product space. Both indices increase as the pairwise distances between the subsets Xi become large compared to the diameters of Xi Maximally separated partitions p' are defined and it is shown that as the indices of p' increase without bound, the characteristic functions of Xi' in P' are approximated more and more closely by the membership functions in fuzzy partitions which minimize certain fuzzy extensions of the k-means squared error criterion function.
A systematic review of the colorectal microbiome in adult cystic fibrosis patients

Cystic fibrosis (CF) is a hereditary, life‐limiting, multi‐system condition that results in chronic respiratory infections, pancreatic insufficiency and intestinal inflammation. Evidence indicates that CF patients develop colorectal cancer (CRC) earlier and more often than the general population. Intestinal dysbiosis resulting from genetics and CF treatment is a contributing factor. This systematic review aims to evaluate the literature to compare the microbiome of adult CF patients to non‐CF patients and to assess if these changes correspond with known CRC microbiome alterations.
Sharps and needlestick injuries within the operating room: Risk prone procedures and prevalence meta-analysis

Background: Sharps and needlestick injuries pose a serious risk to operating theatre personnel with considerable morbidity, mortality and healthcare implications. The cost of prophylaxis and post-exposure treatment is a significant institutional economic burden. Aim: The aim of the review was to identify the prevalence of sharps and needlestick injury within the operating theatre and to establish the most common critical steps. Method: A systematic literature search was conducted. Abstracts of all studies published in English from 2015 onwards exploring sharps and needlestick injury within the operating theatre were reviewed. Primary outcome measure was sharps and needlestick injury prevalence. Secondary outcome measures included operational steps resulting in sharps and needlestick injury and costs of sharps and needlestick injury management. Results: Sixteen studies were identified and included in analysis. Cross-sectional studies reported a pooled prevalence of 41.5% (n = 537; 95% confidence interval = 15.961 to 70.220). Retrospective data analysis reported an annualised prevalence of 5.027% (95% confidence interval = 0.676 to 13.073) on a total pooled sample population of 12,929. Further analysis of operational steps identified a 22% prevalence (n= 3460; 95% confidence interval = 14.2 to 31.3) of sharps and needlestick injury occurring during a procedure involving handing or receiving an instrument. Conclusion: Sharps and needlestick injuries are a significant but preventable risk in the operating theatre. Further research into the development of safety devices to reduce injury during instrument transfer is paramount.
Geographical variations of food insecurity and its associated factors in Bangladesh: Evidence from pooled data of seven cross-sectional surveys

Food insecurity has multiple negative effects on maternal and child health and nutritional outcomes. There is a dearth of up-to-date evidence on the prevalence of food insecurity in Bangladesh based on geographical variations. We investigated the prevalence of food insecurity based on geographical variations and its associated factors. We pooled data from seven cross-sectional surveys conducted in 15,009 households from March 2015 to May 2018. This study was a part of the evaluation of the Maternal Infant Young Child Nutrition Phase 2 programme implemented by BRAC, one of the largest international non-governmental organizations located in Bangladesh that covered rural areas in 26 districts and two urban slums in Dhaka, Bangladesh. We used Household Food Insecurity Access Scale (a widely used scale to measure household food insecurity) to estimate the food insecurity status from the data collected through a face-to-face interview using a structured questionnaire. Hot spot analysis was conducted using the Getis-Ord Gi* statistic. The multiple logistic regression model was applied to explore the associated factors of food insecurity. The food insecurity hotspots were in the northwestern, central-southwestern, and coastal districts of Bangladesh. The overall prevalence of mild, moderate, and severe food insecurity were 12.7%, 13.8%, and 3.5%, respectively. In the adjusted model, household heads and caregivers of children with five or more years of schooling had respectively 42% (adjusted odds ratio (AOR): 0.58, 95% confidence interval (CI): 0.52, 0.64) and 46% (AOR: 0.54; 95% CI: 0.49, 0.61) less likelihood to suffer from food insecurity. Households in the middle (AOR: 0.58, 95% CI: 0.52, 0.65) and rich (AOR: 0.32, 95% CI: 0.28, 0.36) wealth status had lower odds of food insecurity. Food insecurity is widely spread in rural districts of Bangladesh and the degree of vulnerability is higher among the households of the northwestern, central-southwestern, and coastal areas of Bangladesh. Comprehensive interventions including strategies for poverty reduction and education for all might be effective to reduce food insecurity at rural households in Bangladesh.