Miracle-dz/UniFAR-FTUs huggingface dataset:
In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

Ovarian cancer (OC) is a disease of major concern with a survival rate of about 40% at five years. This is attributed to the lack of visible and reliable symptoms during the onset of the disease, which leads over 80% of patients to be diagnosed at advanced stages. This implies that metastatic activity has advanced to the peritoneal cavity. It is associated with both genetic and phenotypic heterogeneity, which considerably increase the risks of relapse and reduce the survival rate. To understand ovarian cancer pathophysiology and strengthen the ability for drug screening, further development of relevant in vitro models that recapitulate the complexity of OC microenvironment and dynamics of OC cell population is required. In this line, the recent advances of tridimensional (3D) cell culture and microfluidics have allowed the development of highly innovative models that could bridge the gap between pathophysiology and mechanistic models for clinical research. This review first describes the pathophysiology of OC before detailing the engineering strategies developed to recapitulate those main biological features.
Two-photon absorption in GaN, GaInN, and GaAlN alloys

We have carried out full band-structure-based studies of two-photon absorption coefficients in the GaN compound, and GaInN and GaAlN alloys. An accurate Hamiltonian and resulting wave functions and band structures are used in the calculations. The coefficients in GaN calculated without any adjustable parameters agree very well with recent experiments. We further show that the two-photon absorption coefficients can be increased or decreased by adding In or Al to the GaN compound. The spectral variation of the absorption coefficient can be explained in terms of the alloy-induced changes in the band gap and joint density of states.
Moments of maximum likelihood estimators in the growth curve model

The Growth Curve model (POTTHOFF ROY 1964) is studied. We obtain moments of order up to 6 for the maximum likelihood estimator of B in E [X] = ABC and the first and second ordr moments of the maximum likelihood estimator of the dispersion matrix are obtained. In order to apply the moment expressions the moments are in turn estimated. all results of the paper are based on moments for matrix normally, WISHART and inverted WISHART distributed variables. An extended model, allowing for covariates, is alos treated
Honey as a Strategy to Fight Candida tropicalis in Mixed-Biofilms with Pseudomonas aeruginosa

Fungal contaminations with Candida species are commonly responsible for several infections, especially when associated to bacteria. The therapeutic approach commonly used is being compromised due to microbial resistances of these microorganisms to antimicrobial agents, especially in biofilm. The use of honey as an antimicrobial agent has been emerging as a valuable solution and proving its potential in planktonic and in biofilm cells. This work aims to assess the effect of different honeys on biofilms of Candida tropicalis and Pseudomonas aeruginosa. The effect of Portuguese heather (PH) and manuka honeys on planktonic growth of Candida was initially evaluated by determination of the minimum inhibitory concentrations (MIC). Then, the same effect was evaluated in mixed biofilms, by colony-forming units numeration and fluorescence microscopy. The combinations of honey plus fluconazole and gentamicin were also tested. The results showed that the honeys tested enabled a great reduction of C. tropicalis, both in planktonic (12.5% and 25% of MIC for PH and manuka) and in biofilm. In polymicrobial biofilms, the use of PH and manuka honeys was revealed to be a promising choice and an alternative treatment, since they were able to reduce cells from both species. No synergistic effect was observed in antimicrobial combinations assays against polymicrobial biofilms.
Kidney Structural Features from Living Donors Predict Graft Failure in the Recipient.

BACKGROUND Nephrosclerosis, nephron size, and nephron number vary among kidneys transplanted from living donors. However, whether these structural features predict kidney transplant recipient outcomes is unclear. METHODS Our study used computed tomography (CT) and implantation biopsy to investigate donated kidney features as predictors of death-censored graft failure at three transplant centers participating in the Aging Kidney Anatomy study. We used global glomerulosclerosis, interstitial fibrosis/tubular atrophy, artery luminal stenosis, and arteriolar hyalinosis to measure nephrosclerosis; mean glomerular volume, cortex volume per glomerulus, and mean cross-sectional tubular area to measure nephron size; and calculations from CT cortical volume and glomerular density on biopsy to assess nephron number. We also determined the death-censored risk of graft failure with each structural feature after adjusting for the predictive clinical characteristics of donor and recipient. RESULTS The analysis involved 2293 donor-recipient pairs. Mean recipient follow-up was 6.3 years, during which 287 death-censored graft failures and 424 deaths occurred. Factors that predicted death-censored graft failure independent of both donor and recipient clinical characteristics included interstitial fibrosis/tubular atrophy, larger cortical nephron size (but not nephron number), and smaller medullary volume. In a subset with 12 biopsy section slides, arteriolar hyalinosis also predicted death-censored graft failure. CONCLUSIONS Subclinical nephrosclerosis, larger cortical nephron size, and smaller medullary volume in healthy donors modestly predict death-censored graft failure in the recipient, independent of donor or recipient clinical characteristics. These findings provide insights into a graft's "intrinsic quality" at the time of donation, and further support the use of intraoperative biopsies to identify kidney grafts that are at higher risk for failure.
Single-Phase Laminar Forced Convection in Microchannels With Rounded Corners

This work investigates the frictional and heat transfer behavior of laminar, fully developed flow in microchannels with trapezoidal and rectangular cross-section and rounded corners under boundary conditions of uniform heat flux along the channel length and perimeter and uniform temperature on the heated perimeter of each cross section. The equations of momentum and energy are solved numerically using the least square method, and the results are validated with analytical data, when available. The runs have been carried out for different aspect ratios and nondimensional radii of curvature, with either all sides or three sides heated, one short side adiabatic for rectangular geometries and three sides heated, the longest one adiabatic for trapezoidal geometries. The Poiseuille and Nusselt numbers are reported and show, for the rectangular cross-section heated on all sides, a maximum increase for the highest value of the aspect ratio with increments in the Poiseuille and Nusselt numbers of about 11% and 16%, respectively, for values of the nondimensional radius of curvature of 0.5, increasing as the geometry approaches the circular duct (12.5% and 21%). The increase is less pronounced as the aspect ratio decreases and also when only three sides are heated (maximum increase of the Nusselt number around 10% for the latter); in the case of trapezoidal geometry the effects of rounding the corners are almost negligible (a maximum increase in the Nusselt number of around 2%).
Additive Potentiation of R334W-CFTR Function by Novel Small Molecules

The R334W (c.1000C>T, p.Arg334Trp) is a rare cystic fibrosis (CF)-causing mutation for which no causal therapy is currently approved. This mutation leads to a significant reduction of CF transmembrane conductance regulator (CFTR) channel conductance that still allows for residual function. Potentiators are small molecules that interact with CFTR protein at the plasma membrane to enhance CFTR-dependent chloride secretion, representing thus pharmacotherapies targeting the root cause of the disease. Here, we generated a new CF bronchial epithelial (CFBE) cell line to screen a collection of compounds and identify novel potentiators for R334W-CFTR. The active compounds were then validated by electrophysiological assays and their additive effects in combination with VX-770, genistein, or VX-445 were exploited in this cell line and further confirmed in intestinal organoids. Four compounds (LSO-24, LSO-25, LSO-38, and LSO-77) were active in the functional primary screen and their ability to enhance R334W-CFTR-dependent chloride secretion was confirmed using electrophysiological measurements. In silico ADME analyses demonstrated that these compounds follow Lipinski’s rule of five and are thus suggested to be orally bioavailable. Dose–response relationships revealed nevertheless suboptimal efficacy and weak potency exerted by these compounds. VX-770 and genistein also displayed a small potentiation of R334W-CFTR function, while VX-445 demonstrated no potentiator activity for this mutation. In the R334W-expressing cell line, CFTR function was further enhanced by the combination of LSO-24, LSO-25, LSO-38, or LSO-77 with VX-770, but not with genistein. The efficacy of potentiator VX-770 combined with active LSO compounds was further confirmed in intestinal organoids (R334W/R334W genotype). Taken together, these molecules were demonstrated to potentiate R334W-CFTR function by a different mechanism than that of VX-770. They may provide a feasible starting point for the design of analogs with improved CFTR-potentiator activity.
A label-free surface-enhanced Raman spectroscopy biosensor for on-site breast cancer detection using human tears.

Surface-enhanced Raman scattering (SERS) is an ultrasensitive molecular screening technique with greatly enhanced Raman scattering signals from trace amounts of analytes near plasmonic nanostructures. However, research on the development of a sensor that balances signal enhancement, reproducibility, and uniformity has not yet been proposed for practical applications. In this study, we demonstrate the potential of the practical application for detecting or predicting asymptomatic breast cancer from human tears using a portable Raman spectrometer with an identification algorithm based on multivariate statistics. This potentiality was realized through the fabrication of a plasmonic SERS substrate equipped with a well-aligned, gold-decorated, hexagonal-close-packed polystyrene (Au/HCP-PS) nanosphere monolayer that provided femtomole-scale detection, giga-scale enhancement, and <5% relative standard deviation for reliability and reproducibility, regardless of the measuring site. Our results can provide a first step toward developing a non-invasive, real-time screening technology for detecting asymptomatic tumors and preventing tumor recurrence.
Real-Time Transmission Control for Multichannel NOMA Random Access Systems

To improve the throughput per channel in multichannel nonorthogonal multiple access (NOMA) random access (RA) system, users (re)transmit their packet to one of the channels using transmit power control such that the receive power of the packets at the base station (BS) can be one of the predefined levels called target receive power (TRP). The BS decodes the received packets in the descending order of the TRPs at each slot using successive interference cancellation (SIC). This work proposes the real-time transmission algorithm for users to (re)transmit their packet for maximization of the system throughput. To do this, the BS estimates the number of backlogged users in real time and adjusts and broadcasts the throughput-optimal (re)transmission probability in the algorithm. We analyze the average RA delay performance of the proposed algorithm and demonstrate its performance even with time-varying traffic.
Measurement of mitochondrial respiration in permeabilized fish gills

ABSTRACT Physiological investigations of fish gills have traditionally centred on the two principal functions of the gills: gas exchange and ion regulation. Mitochondrion-rich cells (MRCs) are primarily found within the gill filaments of fish, and are thought to proliferate in order to increase the ionoregulatory capacity of the gill in response to environmentally induced osmotic challenges. However, surprisingly little attention has been paid to the metabolic function of mitochondria within fish gills. Here, we describe and validate a simple protocol for the permeabilization of fish gills and subsequent measurement of mitochondrial respiration rates in vitro. Our protocol requires only small tissue samples (8 mg), exploits the natural structure of fish gills, does not require mechanical separation of the gill tissue (so is relatively quick to perform), and yields accurate and highly reproducible measurements of respiration rates. It offers great potential for the study of mitochondrial function in gills over a wide range of fish sizes and species. Summary: Outline of a simple protocol for permeabilization of fish gills and measurement of mitochondrial physiology; this technique exploits the natural structure of gills and does not require mechanical separation.
Running Speed at Predicted Maximal Heart Rate as an Assessment of Maximal Aerobic Capacity in Trained Teenaged Runners

Purpose: The purpose of this study was to examine whether the running speed (vHRmax.pred) corresponding to a HRmax predicted by the formula [220-age (yrs)], is a useful physiologic index of endurance among trained young runners in a specified age group (12-20 yrs). Correlations were analyzed between vHRmax.pred and Vo2max, vVo2max (running speed corresponding to Vo2max,), vLT (running speed at a blood lactate level of 4 mmol·L−1), vHRmax.meas (running speed at the measured HRmax), and competitive 1500 and 3000-m performance times in 43 endurance-trained, teenaged runners (25 males, 18 females). Methods: Physiological variables (Vo2, HR, and La) were measured during progressive sub-maximal and maximal treadmill running. Running speeds corresponding to Vo2max, HRmax.meas, and HRmax.pred were estimated from regressions relating each individual's running speed to Vo2 and HR.Results: With a few exceptions, gender-specific correlation coefficients were significant between Vo2max, vVo2max, vLT, vHRmax.meas, and vHRmax.pred, with values ranging from 0.41 to 0.93 (p<0.05). vHRmax.pred was a significant predictor of running performance for both 1500 and 3000-m events (r=−0.62 and −0.52 in males, and −0.66 and −0.80 in females, respectively). Conclusion: The results suggested that vHRmax.pred is a useful predictor of endurance running performance in trained teenaged runners. This fact also suggested the possibility of developing an index of endurance running performance in untrained teenagers.
Adipose-Derived Mesenchymal Stem Cells Inhibit JNK-Mediated Mitochondrial Retrograde Pathway to Alleviate Acetaminophen-Induced Liver Injury

Acetaminophen (APAP) is the major cause of drug-induced liver injury, with limited treatment options. APAP overdose invokes excessive oxidative stress that triggers mitochondria-to-nucleus retrograde pathways, contributing to APAP-induced liver injury (AILI). Mesenchymal stem cell therapy is a promising tool for acute liver failure. Therefore, the purpose of this study was to investigate the beneficial effects of adipose-derived mesenchymal stem cell (AMSC) therapy on AILI and reveal the potential therapeutic mechanisms. C57BL/6 mice were used as the animal model and AML12 normal murine hepatocytes as the cellular model of APAP overdose. Immunohistochemical staining, Western blotting, immunofluorescence staining, and RNA sequencing assays were used for assessing the efficacy and validating mechanisms of AMSC therapy. We found AMSC therapy effectively ameliorated AILI, while delayed AMSC injection lost its efficacy related to the c-Jun N-terminal kinase (JNK)-mediated mitochondrial retrograde pathways. We further found that AMSC therapy inhibited JNK activation and mitochondrial translocation, reducing APAP-induced mitochondrial damage. The downregulation of activated ataxia telangiectasia-mutated (ATM) and DNA damage response proteins in AMSC-treated mouse liver indicated AMSCs blocked the JNK-ATM pathway. Overall, AMSCs may be an effective treatment for AILI by inhibiting the JNK-ATM mitochondrial retrograde pathway, which improves APAP-induced mitochondrial dysfunction and liver injury.
A Track from Attachment to Resilience During the Covid 19 Pandemic: Romantic Relationship Tendency

The COVID-19 pandemic involves some psychosocial problems in addition to physical risks, and resilience appears to be a critical feature to cope with these problems. This study aims to present a model examining the relationships between the psychological tendencies associated with romantic relationships, attachment styles, social media usage, and resilience during the COVID-19 pandemic. These relationships were investigated in a sample of unmarried adults (n = 289) with a romantic relationship history of at least six months. The average age of the participants was 27 years. As a result of structural equation modelling, anxious and avoidant attachment styles had no effect on the purpose of social media usage and the purpose of social media usage had no effect on psychological resilience. Also, anxious and avoidant attachment styles affected resilience, and this effect was fully mediated by psychological tendencies associated with romantic relationships. The findings reveal the necessity to consider the psychological tendencies associated with romantic relationships in studies aimed at increasing resilience.
Estimating Transformations for Regression via Additivity and Variance Stabilization

Abstract I propose a method for the nonparametric estimation of transformations for regression. It is much more flexible than the familiar Box-Cox procedure, allowing general smooth transformations of the variables, and is similar to the ACE (alternating conditional expectation) algorithm of Breiman and Friedman (1985). The ACE procedure uses scatterplot smoothers in an iterative fashion to find the maximally correlated transformations of the variables. Like ACE, my proposal can incorporate continuous, categorical, or periodic variables, or any mixture of these types. The method differs from ACE in that it uses a (nonparametric) variance-stabilizing transformation for the response variable. The technique seems to alleviate many of the anomalies that ACE suffers with regression data, including the inability to reproduce model transformations and sensitivity to the marginal distribution of the predictors. I provide several examples, including an analysis of the “brain and body weight” data and some data on .
Finding Earth-size planets in the habitable zone: the Kepler Mission

Abstract The Kepler Mission is a space-based mission whose primary goal is to detect Earth-size and smaller planets in the habitable zone of solar-like stars. The mission will monitor more than 100,000 stars for transits with a differential photometric precision of 20 ppm at V=12 for a 6.5 hour transit. It will also provide asteroseismic results on several thousand dwarf stars. It is specifically designed to continuously observe a single field of view of greater than 100 square degrees for 3.5 or more years. This overview describes the mission design, its goals and capabilities, the measured performance for those photometer components that have now been tested, the Kepler Input Catalog, an overview of the analysis pipeline, the plans for the Follow-up Observing Program to validate the detections and characterize the parent stars, and finally, the plans for the Guest Observer and Astrophysical Data Program.
A molecular dynamics study of the nematic phase of 4-n-pentyl-4'-cyanobiphenyl

Abstract Preliminary molecular dynamics simulations of the nematic phase of 4-n-pentyl-4′-cyanobiphenyl are described. The simulations include all molecular degrees of freedom. The influence of the molecular dipole moment is investigated by comparing simulations with and without a charge distribution on the molecules. Inclusion of the charge distribution is found to lead to a slight broadening of the orientational distribution function, in qualitative agreement with Raman measurements of the orientational order parameters.
An ADC-Less RRAM-Based Computing-in-Memory Macro With Binary CNN for Efficient Edge AI

Resistive random-access memory (RRAM) based non-volatile computing-in-memory (nvCIM) has been regarded as a promising solution to enable efficient data-intensive artificial intelligence (AI) applications on resource-limited edge systems. However, existing weighted-current summation-based nvCIM suffers from device non-idealities and significant time, storage, and energy overheads when processing high-precision analog signals. To address these issues, we propose a 3T2R digital nvCIM macro for a fully hardware-implemented binary convolutional neural network (HBCNN), focusing on accelerating edge AI applications at low weight precision. By quantizing the voltage-division results of RRAMs through inverters, the 3T2R macro provides a stable rail-to-rail output without analog-to-digital converters or sensing amplifiers. Moreover, both batch normalization and sign activation are integrated on-chip. The hybrid simulation results show that the proposed 3T2R digital macro achieves an 86.2% (95.6%) accuracy on the CIFAR-10 (MNIST) dataset, corresponding to a 4.7% (1.9%) accuracy loss compared to the software baselines, which also feature a peak energy efficiency of 51.3 TOPS/W and a minimum latency of 8 ns, realizing an energy-efficient, low-latency, and robust AI processor.
Using a digital video camera to examine coupled oscillations

In our previous paper (Debowska E, Jakubowicz S and Mazur Z 1999 Eur. J. Phys. 20 89-95), thanks to the use of an ultrasound distance sensor, experimental verification of the solution of Lagrange equations for longitudinal oscillations of the Wilberforce pendulum was shown. In this paper the sensor and a digital video camera were used to monitor and measure the changes of both the pendulum's coordinates (vertical displacement and angle of rotation) simultaneously. The experiments were performed with the aid of the integrated software package COACH 5. Fourier analysis in Microsoft Excel 97 was used to find normal modes in each case of the measured oscillations. Comparison of the results with those presented in our previous paper (as given above) leads to the conclusion that a digital video camera is a powerful tool for measuring coupled oscillations of a Wilberforce pendulum. The most important conclusion is that a video camera is able to do something more than merely register interesting physical phenomena - it can be used to perform measurements of physical quantities at an advanced level.
Draft Genome Sequence of an Isolate of Extensively Drug-Resistant Mycobacterium tuberculosis from Nepal

Extensively drug-resistant (XDR) Mycobacterium tuberculosis has become a challenge to the treatment of tuberculosis (TB) in several countries, including Nepal. Here, we report for the first time the draft genome sequence of an isolate of XDR-TB collected in Nepal and describe single-nucleotide variations associated with its extensively drug-resistant phenotype. ABSTRACT Extensively drug-resistant (XDR) Mycobacterium tuberculosis has become a challenge to the treatment of tuberculosis (TB) in several countries, including Nepal. Here, we report for the first time the draft genome sequence of an isolate of XDR-TB collected in Nepal and describe single-nucleotide variations associated with its extensively drug-resistant phenotype.