Miracle-dz/UniFAR-FTUs huggingface dataset:
Gafchromic EBT‐XD film: Dosimetry characterization in high‐dose, volumetric‐modulated arc therapy

Radiochromic films are important tools for assessing complex dose distributions. Gafchromic EBT‐XD films have been designed for optimal performance in the 40–4,000 cGy dose range. We investigated the dosimetric characteristics of these films, including their dose‐response, postexposure density growth, and dependence on scanner orientation, beam energy, and dose rate with applications to high‐dose volumetric‐modulated arc therapy (VMAT) verification. A 10 MV beam from a TrueBeam STx linear accelerator was used to irradiate the films with doses in the 0–4,000 cGy range. Postexposure coloration was analyzed at postirradiation times ranging from several minutes to 48 h. The films were also irradiated with 6 MV (dose rate (DR): 600 MU/min), 6 MV flattening filter‐free (FFF) (DR: 1,400 MU/ min), and 10 MV FFF (DR: 2,400 MU/min) beams to determine the energy and dose‐rate dependence. For clinical examinations, we compared the dose distribution measured with EBT‐XD films and calculated by the planning system for four VMAT cases. The red channel of the EBT‐XD film exhibited a wider dynamic range than the green and blue channels. Scanner orientation yielded a variation of ∼3% in the net optical density (OD). The difference between the film front and back scan orientations was negligible, with variation of ∼1.3% in the net OD. The net OD increased sharply within the first 6 hrs after irradiation and gradually afterwards. No significant difference was observed for the beam energy and dose rate, with a variation of ∼1.5% in the net OD. The gamma passing rates (at 3%, 3 mm) between the film‐ measured and treatment planning system (TPS)‐calculated dose distributions under a high dose VMAT plan in the absolute dose mode were more than 98.9%. PACS number(s): 87.56 Fc
RansomWall: A layered defense system against cryptographic ransomware attacks using machine learning

Recent worldwide cybersecurity attacks caused by Cryptographic Ransomware infected systems across countries and organizations with millions of dollars lost in paying extortion amounts. This form of malicious software takes user files hostage by encrypting them and demands a large ransom payment for providing the decryption key. Signature-based methods employed by Antivirus Software are insufficient to evade Ransomware attacks due to code obfuscation techniques and creation of new polymorphic variants everyday. Generic Malware Attack vectors are also not robust enough for detection as they do not completely track the specific behavioral patterns shown by Cryptographic Ransomware families. This work based on analysis of an extensive dataset of Ran-somware families presents RansomWall, a layered defense system for protection against Cryptographic Ransomware. It follows a Hybrid approach of combined Static and Dynamic analysis to generate a novel compact set of features that characterizes the Ransomware behavior. Presence of a Strong Trap Layer helps in early detection. It uses Machine Learning for unearthing zero-day intrusions. When initial layers of RansomWall tag a process for suspicious Ransomware behavior, files modified by the process are backed up for preserving user data until it is classified as Ransomware or Benign. We implemented RansomWall for Microsoft Windows operating system (the most attacked OS by Cryptographic Ransomware) and evaluated it against 574 samples from 12 Cryptographic Ransomware families in real-world user environments. The testing of RansomWall with various Machine Learning algorithms evaluated to 98.25% detection rate and near-zero false positives with Gradient Tree Boosting Algorithm. It also successfully detected 30 zero-day intrusion samples (having less than 10% detection rate with 60 Security Engines linked to VirusTotal).
Pseudo-Noise Sequence Based Synchronization for Generalized Frequency Division Multiplexing in 5G Communication System

As one of the candidate waveforms for 5G, generalized frequency division multiplexing (GFDM) is a flexible non-orthogonal multicarrier technique. Similar to orthogonal frequency division multiplexing, GFDM communication system suffers from symbol timing offset (STO) and carrier frequency offset (CFO) caused by multipath effect and Doppler shift. In this paper, we design a pseudo noise (PN) based preamble and propose an approach for signal synchronization. The preamble is composed of two identical PN sequences whose autocorrelation and cross-correlation are better than cyclic prefix (CP). STO can be detected by calculating the correlation coefficient between the local PN sequence of receiver and the corresponding preamble part of sliding window. These two adjacent PN sequences can be used to calculate phase offset value by Fourier transform, and then obtain CFO value. Simulation results demonstrate that, compared with conventional CP-based synchronization, the proposed approach has the lower mean square error and symbol error rate at the cost of just a little bit more bandwidth.
Exact geometry solid-shell element based on a sampling surfaces technique for 3D stress analysis of doubly-curved composite shells

Abstract A hybrid-mixed ANS four-node shell element by using the sampling surfaces (SaS) technique is developed. The SaS formulation is based on choosing inside the nth layer In not equally spaced SaS parallel to the middle surface of the shell in order to introduce the displacements of these surfaces as basic shell variables. Such choice of unknowns with the consequent use of Lagrange polynomials of degree In − 1 in the thickness direction for each layer permits the presentation of the layered shell formulation in a very compact form. The SaS are located inside each layer at Chebyshev polynomial nodes that allows one to minimize uniformly the error due to the Lagrange interpolation. To implement the efficient analytical integration throughout the element, the enhanced ANS method is employed. The proposed hybrid-mixed four-node shell element is based on the Hu-Washizu variational equation and exhibits a superior performance in the case of coarse meshes. It could be useful for the 3D stress analysis of thick and thin doubly-curved shells since the SaS formulation gives the possibility to obtain numerical solutions with a prescribed accuracy, which asymptotically approach the exact solutions of elasticity as the number of SaS tends to infinity.
An organ and effective dose study of XVI and OBI cone‐beam CT systems

The main purpose of this work was to quantify patient organ doses from the two kilovoltage cone beam computed tomography (CBCT) systems currently available on medical linear accelerators, namely the X‐ray Volumetric Imager (XVI, Elekta Oncology Systems) and the On‐Board Imager (OBI, Varian Medical Systems). Organ dose measurements were performed using a fiber‐optic coupled (FOC) dosimetry system along with an adult male anthropomorphic phantom for three different clinically relevant scan sites: head, chest, and pelvis. The FOC dosimeter was previously characterized at diagnostic energies by Hyer et al. [Med Phys 2009;36(5):1711–16] and a total uncertainty of approximately 4% was found for in‐phantom dose measurements. All scans were performed using current manufacturer‐installed clinical protocols and appropriate bow‐tie filters. A comparison of image quality between these manufacturer‐installed protocols was also performed using a Catphan 440 image quality phantom. Results indicated that for the XVI, the dose to the lens of the eye (1.07 mGy) was highest in a head scan, thyroid dose (19.24 mGy) was highest in a chest scan, and gonad dose (29 mGy) was highest in a pelvis scan. For the OBI, brain dose (3.01 mGy) was highest in a head scan, breast dose (5.34 mGy) was highest in a chest scan, and gonad dose (34.61 mGy) was highest in a pelvis scan. Image quality measurements demonstrated that the OBI provided superior image quality for all protocols, with both better spatial resolution and low‐contrast detectability. The measured organ doses were also used to calculate a reference male effective dose to allow further comparison of the two machines and imaging protocols. The head, chest, and pelvis scans yielded effective doses of 0.04, 7.15, and 3.73 mSv for the XVI, and 0.12, 1.82, and 4.34 mSv for the OBI, respectively. PACS number: 87.57. uq
Prion Strains and Transmission Barrier Phenomena

Several experimental evidences show that prions are non-conventional pathogens, which physical support consists only in proteins. This finding raised questions regarding the observed prion strain-to-strain variations and the species barrier that happened to be crossed with dramatic consequences on human health and veterinary policies during the last 3 decades. This review presents a focus on a few advances in the field of prion structure and prion strains characterization: from the historical approaches that allowed the concept of prion strains to emerge, to the last results demonstrating that a prion strain may in fact be a combination of a few quasi species with subtle biophysical specificities. Then, we will focus on the current knowledge on the factors that impact species barrier strength and species barrier crossing. Finally, we present probable scenarios on how the interaction of strain properties with host characteristics may account for differential selection of new conformer variants and eventually species barrier crossing.
An investigation of eye lens dose for gamma knife treatments of trigeminal neuralgia

Stereotactic Gamma Knife radiosurgery has been widely used for treating trigeminal neuralgia (TN). A single large fractional dose of 7000 to 9000 cGy is commonly prescribed as the maximum dose for these treatments. For this reason, if a small percentage of the prescribed dose such as 2–3 % scattered to the eye, it could reach or even exceed the tolerance dose of the lens. For several TN cases, we found that the Leksell Gamma Plan system calculates the lens dose about 0.5–2 % of the maximum dose independent of the use of eye shielding. These dose values are significantly high and it motivated us to investigate the lens dose for the TN patients treated with stereotactic Gamma Knife radiosurgery. Phantom studies and in vivo dosimetry measurements were carried out for six patients treated at our institution. The average dose to the lens ipsilateral to the treated nerve was measured to be 7.7±0.6 cGy. Based on the biological model of Lyman and Emami [Int. J. Radiat. Oncol. Biol. Phys. 21, 109–122 (1991)], the probability of the lens complication (cataract) was determined to be 0.1%. Our findings suggest that few TN patients would develop cataracts after receiving Gamma Knife radiosurgery. PACS number(s): 87.53. –j, 87.66. –a
Preventive effect of Dioscorea japonica on squamous cell carcinoma of mouse skin involving down-regulation of prostaglandin E2 synthetic pathway

Hyperproduced prostaglandin E2 by cyclooxygenase-2 and microsomal prostaglandin E synthase-1 evokes several pathophysiological responses such as inflammation and carcinogenesis. Our recent study demonstrated that Dioscorea japonica extract suppressed the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 and induced apoptosis in lung carcinoma A549 cells. In the present study, we investigated the effects of Dioscorea japonica on squamous cell carcinoma of mouse skin. Dioscorea japonica feeding and Dioscorea japonica extract topical application suppressed the expression of cyclooxygenase-2, microsomal prostaglandin E synthase-1, interleukin-1β and interleukin-6 and inhibited tumor formation, hyperplasia and inflammatory cell infiltration. Immunohistochemical analyses showed the immunoreactivities of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in tumor keratinocytes and stronger immunoreactivities of cyclooxygenase-2 and hematopoietic prostaglandin D synthase in epidermal dendritic cells (Langerhans cells). Treatment with Dioscorea japonica decreased the immunoreactivity of cyclooxygenase-2 and microsomal prostaglandin E synthase-1. These results indicate that Dioscorea japonica may have inhibitory effects on inflammation and carcinogenesis via suppression of the prostaglandin E2 synthetic pathway.
Discriminant Analysis-Based Dimension Reduction for Hyperspectral Image Classification: A Survey of the Most Recent Advances and an Experimental Comparison of Different Techniques

Hyperspectral imagery contains hundreds of contiguous bands with a wealth of spectral signatures, making it possible to distinguish materials through subtle spectral discrepancies. Because these spectral bands are highly correlated, dimensionality reduction, as the name suggests, seeks to reduce data dimensionality without losing desirable information. This article reviews discriminant analysisbased dimensionality-reduction approaches for hyperspectral imagery, including typical linear discriminant analysis (LDA), state-of-the-art sparse graph-based discriminant analysis (SGDA), and their extensions.
A novel and independent method for time‐resolved gantry angle quality assurance for VMAT

Abstract Volumetric‐modulated arc therapy (VMAT) treatment delivery requires three key dynamic components; gantry rotation, dose rate modulation, and multi‐leaf collimator motion, which are all simultaneously varied during the delivery. Misalignment of the gantry angle can potentially affect clinical outcome due to the steep dose gradients and complex MLC shapes involved. It is essential to develop independent gantry angle quality assurance (QA) appropriate to VMAT that can be performed simultaneously with other key VMAT QA testing. In this work, a simple and inexpensive fully independent gantry angle measurement methodology was developed that allows quantitation of the gantry angle accuracy as a function of time. This method is based on the analysis of video footage of a “Double dot” pattern attached to the front cover of the linear accelerator that consists of red and green circles printed on A4 paper sheet. A standard mobile phone is placed on the couch to record the video footage during gantry rotation. The video file is subsequently analyzed and used to determine the gantry angle from each video frame using the relative position of the two dots. There were two types of validation tests performed including the static mode with manual gantry angle rotation and dynamic mode with three complex test plans. The accuracy was 0.26° ± 0.04° and 0.46° ± 0.31° (mean ± 1 SD) for the static and dynamic modes, respectively. This method is user friendly, cost effective, easy to setup, has high temporal resolution, and can be combined with existing time‐resolved method for QA of MLC and dose rate to form a comprehensive set of procedures for time‐resolved QA of VMAT delivery system.
Quantification of the Effect of Array Element Pitch on Imaging Performance

This paper investigates how the pitch of elements in periodic ultrasonic arrays is related to their imaging performance, with particular emphasis on imaging artifacts (grating lobes) arising from discrete spatial sampling. Although the classical Nyquist rules for array element pitch are well known, they only provide the limiting condition needed to eliminate grating lobes from an array with an infinitely large aperture at a single frequency. Physical arrays have finite-sized apertures and most applications employ broadband pulses. For these reasons, grating lobe artifacts are always present at some level, and practical array design is, therefore, based on suppressing grating lobe artifacts to a level appropriate to a given application. In this paper, a theoretical framework is developed that enables the point spread function of a periodic imaging array to be decomposed into the sum of contributions from a main lobe and different orders of grating lobes, thus allowing grating lobe artifacts to be unambiguously quantified. Numerical simulations are used to analyze the performance of 1-D linear arrays in both far-field (steering only) and near-field (focusing only) scenarios, and design guidelines are deduced. It is shown that in general, the classical Nyquist rules are overly conservative and that the pitch of an array can be increased without significantly compromising image quality, provided that certain constraints on ray angles are implemented in the imaging algorithm. Experimental examples are shown that illustrate the practical application to arrays in two configurations.
Discharge Delays for Patients Requiring In-Hospital Guardianship: A Cohort Analysis

Objective: To assess nonclinical factors delaying hospital discharge of guardianship patients. Data: Utilization review data over 3 years. Design: Retrospective cohort study. Analysis: Mann–Whitney test was used to compare patients' medically unnecessary days (MUD) of hospitalization with additional subcategories of delays—defined as beyond clinicians' control. Findings: Overall median number of MUD was 19.5; 14 of 48 patients were additionally delayed while awaiting long-term care Medicaid approval (N = 7, 50%), pending insurance (N = 3, 21%), social or transportation difficulties (N = 3, 21%), or preadmission review (N = 1, 7%). The median number of MUD for the 14 delayed patients was 63, a difference of 53 days compared with the routine guardianship cohort (P < .0001) and $5.5M in net revenue opportunity. Conclusions: Nonclinical discharge delays for guardianship patients are costly and potentially unavoidable. Further exploration into policy change is therefore recommended.
Anticancer Activity of Pterostilbene in Human Ovarian Cancer Cell Lines

Background Epithelial ovarian cancer is a major cause of mortality in women and one of the most common gynecologic disorders. Pterostilbene (PTS), a trans-3,5-dimethoxy-4′-hydroxystilbene, was chosen for this work due to its reported effectiveness as a chemotherapeutic agent in cancer studies. In this work, we studied underlying molecular mechanisms of PTS treatment in various ovarian cancer cell lines such as OVCAR8, OV1063, IGROV-1, and SKOV3. Material/Methods We used the cytometric bead array (CBA) method and real-time PCR analysis to analyze the secretion level of tumor necrosis factor alpha (TNF-α) and to measure the TNF-α mRNA expression. NF-kappa B (NF-κB) promoter analysis, Western blot analysis, electrophoresis mobility shift assay (EMSA), and immunostaining analyses were performed to measure the NF-κB activity and other relative proteins levels. Results The PTS treatment decreased the release of TNF-α in IGROV-1 ovarian cancer cells. It also showed significant inhibitory effect on nuclear NF-κB p50, and NF-κB p65 protein levels. Conclusions From the results obtained, we suggest that PTS has the potential to treat ovarian cancer by reducing the level of TNF-α cytokine and to have a limited effect on NF-κB, AKT, and ERK signaling pathways.
Identification of a Continuous Neutralizing Epitope within UL128 of Human Cytomegalovirus

ABSTRACT As human cytomegalovirus (HCMV) is the most common infectious cause of fetal anomalies during pregnancy, development of a vaccine that prevents HCMV infection is considered a global health priority. Although HCMV immune correlates of protection are only poorly defined, neutralizing antibodies (NAb) targeting the envelope pentamer complex (PC) composed of the subunits gH, gL, UL128, UL130, and UL131A are thought to contribute to the prevention of HCMV infection. Here, we describe a continuous target sequence within UL128 that is recognized by a previously isolated potent PC-specific NAb termed 13B5. By using peptide-based scanning procedures, we identified a 13-amino-acid-long target sequence at the UL128 C terminus that binds the 13B5 antibody with an affinity similar to that of the purified PC. In addition, the 13B5 binding site is universally conserved in HCMV, contains a previously described UL128/gL interaction site, and interferes with the 13B5 neutralizing function, indicating that the 13B5 epitope sequence is located within the PC at a site of critical importance for HCMV neutralization. Vaccination of mice with peptides containing the 13B5 target sequence resulted in the robust stimulation of binding antibodies and, in a subset of immunized animals, in the induction of detectable NAb, supporting that the identified 13B5 target sequence constitutes a PC-specific neutralizing epitope. These findings provide evidence for the discovery of a continuous neutralizing epitope within the UL128 subunit of the PC that could be an important target of humoral immune responses that are involved in protection against congenital HCMV infection. IMPORTANCE Neutralizing antibodies (NAb) targeting the human cytomegalovirus (HCMV) envelope pentamer complex (PC) are thought to be important for preventing HCMV transmission from the mother to the fetus, thereby mitigating severe developmental disabilities in newborns. However, the epitope sequences within the PC that are recognized by these potentially protective antibody responses are only poorly defined. Here, we provide evidence for the existence of a highly conserved, continuous, PC-specific epitope sequence that appears to be located within the PC at a subunit interaction site of critical importance for HCMV neutralization. These discoveries provide insights into a continuous PC-specific neutralizing epitope, which could be an important target for a vaccine formulation to interfere with congenital HCMV infection.
Internal-Specific Morphological Analysis of Sciatic Nerve Fibers in a Radiofrequency-Induced Animal Neuropathic Pain Model

This study investigated the reversible effects of pulsed radiofrequency (PRF) treatment at 42°C on the ultrastructural and biological changes in nerve and collagen fibers in the progression of neuropathic pain after rat sciatic nerve injury. Assessments of morphological changes in the extracellular matrices by atomic force microscopy and hematoxylin-eosin, Masson’s trichrome and picrosirius-red staining as well as the expressions of two fibril-forming collagens, types-I and -III, and two inflammatory cytokines, TNF-α and IL-6, were evaluated on day 30 after RF exposure. There were four groups for different RF thermal treatments: no treatment, no current, PRF, and continuous RF (CRF). An RF procedure similar to that used in human clinical trials was used in this study. The CRF treatment at 82°C led to neural and collagen damage by the permanent blockage of sensory nociceptors. The PRF treatment led to excellent performance and high expandability compared to CRF, with effects including slight damage and swelling of myelinated axons, a slightly decreased amount of collagen fibers, swelling of collagen fibril diameters, decreased immunoreactivity of collagen types-I and -III, presence of newly synthesized collagen, and recovery of inflammatory protein immunoreactivity. These evidence-based findings suggest that PRF-based pain relief is responsible for the temporary blockage of nerve signals as well as the preferential destruction of pain-related principal sensory fibers like the Aδ and C fibers. This suggestion can be supported by the interaction between the PRF-induced electromagnetic field and cell membranes; therefore, PRF treatment provides pain relief while allowing retention of some tactile sensation.