Miracle-dz/UniFAR-FTUs huggingface dataset:
Glutamate metabotropic receptors increase a Ca(2+)-activated nonspecific cationic current in CA1 hippocampal neurons.

1. We studied the currents evoked in CA1 pyramidal neurons by the selective metabotropic glutamate receptor (mGluR) agonist 1S,3R-1-aminocyclopentane-1,3-dicarboxylate (1S,3R-ACPD; 100 microM, 2.30-5 min) with the single-electrode voltage-clamp technique in the continuous presence of tetrodotoxin (1 microM), bicuculline (10 microM), 6-cyano-7-nitroquinoxaline-2,3-dione (15 microM), and D-2-amino-5-phosphonovaleric acid (50 microM) to depress action potentials and synaptic activity. Microelectrodes were filled with 3M CsCl or 2 M Cs2SO4. 2. With CsCl-filled microelectrodes, bath application of 1S,3R-ACPD induced an inward current of -308 +/p 50 (SE) pA amplitude [holding potential (VH -60 mV, n = 12)] associated with a conductance decrease (26.5 +/- 5.6%, P < or = 0.0022, n = 12). The current-voltage (I-V) relation of the 1S,3R-ACPD-induced (difference) current investigated using ramp voltage commands from -130 to +10 mV had a V shape with two reversal potentials: -99.6 +/- 3.4 and -17.5 +/- 3.0 mV (n = 12). 3. In contrast, in the presence of external K+ channel blockers (2 mM Ba2+ and 6 mM Cs+ or 25 mM tetraethylammonium, 6 mM Cs+, and 3 mM 4-aminopyridine), 1S,3R-ACPD also generated an inward current, albeit of smaller amplitude (-114.2 +/- 27.5 pA, P < or = 0.003, VH -60 mV, n = 8). This current was associated with a conductance increase (20.7 +/- 3.1%, P < or = 0.0117, n = 8), decreased linearly with depolarization (from -130 to -60 mV), and reversed polarity at an estimated potential of -20.7 +/- 3.6 mV (n = 8). We refer to this current recorded in the presence of K+ channel blockers as IACPD. 4. In the presence of Cd2+ (200 microM, to block voltage-dependent Ca2+ channels that are readily activated in the presence of K+ channel blockers) and a low Ca2+ concentration (100 microM), IACPD decreased linearly from -130 to +10 mV and reversed polarity at -15.8 +/- 8.5 mV (n = 5). (ABSTRACT TRUNCATED AT 250 WORDS)
Ethnography in unstable places : everyday lives in contexts of dramatic political change

Ethnography in Unstable Places is a collection of ethnographic accounts of everyday situations in places undergoing dramatic political transformation. Offering vivid case studies that range from the Middle East and Africa to Europe, Russia, and Southeast Asia, the contributing anthropologists narrate particular circumstances of social and political transformation—in contexts of colonialism, war and its aftermath, social movements, and post–Cold War climates—from the standpoints of ordinary people caught up in and having to cope with the collapse or reconfiguration of the states in which they live. Using grounded ethnographic detail to explore the challenges to the anthropological imagination that are posed by modern uncertainties, the contributors confront the ambiguities and paradoxes that exist across the spectrum of human cultures and geographies. The collection is framed by introductory and concluding chapters that highlight different dimensions of the book’s interrelated themes—agency and ethnographic reflexivity, identity and ethics, and the inseparability of political economy and interpretivism. Ethnography in Unstable Places will interest students and specialists in social anthropology, sociology, political science, international relations, and cultural studies. Contributors. Eve Darian-Smith, Howard J. De Nike, Elizabeth Faier, James M. Freeman, Robert T. Gordon, Carol J. Greenhouse, Nguyen Dinh Huu, Carroll McC. Lewin, Elizabeth Mertz, Philip C. Parnell, Nancy Ries, Judy Rosenthal, Kay B. Warren, Stacia E. Zabusky
Characterization of nascent high density lipoprotein subfractions from perfusates of rat liver.

Nascent high density lipoprotein (HDL) (1.063 less than d less than 1.21 g/ml) was isolated from recirculating rat liver perfusates and separated by heparin-Sepharose chromatography into a nonretained fraction (NR) and a fraction (R) that eluted with 0.5 M NaCl. Fractions NR and R contained 70% and 30% of the nascent HDL protein, respectively. ApoB-containing particles were removed from fraction R by chromatography on concanavalin A. The protein composition of fractions NR and R was 40% and 29%, respectively. Fraction NR contained 25% apoA-I, 11% apoA-IV, 24% apoE, and 38% apoC. Fraction R contained primarily apoE (81% of total protein). The lipid composition of NR and R, respectively, was: triglyceride 44% and 26%, phospholipid 41% and 57%, cholesterol 8% and 13%, and cholesteryl ester 7% and 4%. Fractions NR and R had molecular weights of 400,000 and 860,000, respectively, as calculated from the Stokes radius. Negative staining electron microscopy indicated that both fractions consisted mainly of spherical particles (260-280 A) but some stacked disks were seen in fraction R. Livers perfused by the single-pass technique produced fractions NR and R in the same ratio as livers perfused by recirculation. The apolipoprotein compositions were similar to those in the recirculating perfusion; however, both fractions NR and R had more triglyceride (greater than 50% of total lipid). An HDL fraction was also isolated from liver perfusates by a combination of molecular sieve and heparin-Sepharose affinity chromatography. This HDL contained triglyceride but no apoB, indicating that triglyceride-rich HDL particles are not an artifact of ultracentrifugation. (ABSTRACT TRUNCATED AT 250 WORDS)
Exploring FPGA Capabilities for Building Symmetric Multiprocessor Systems

Advances in FPGA technologies allow designing highly complex systems using on-chip FPGA resources and intellectual property (IP) cores. Furthermore, it is possible to build multiprocessor systems using hard-core or soft-core processors increasing the range of applications that can be implemented on an FPGA. This paper presents an implementation of a symmetric multiprocessor (SMP) system on an FPGA using a vendor provided soft-core processor and a new set of software libraries specially developed for writing applications for this kind of systems. Experimental results show how this approach can improve performance of parallelizable software applications.
Description of ciliary body anatomy and identification of sites for transscleral cyclophotocoagulation in the equine eye.

OBJECTIVE To determine the most appropriate anatomical location for diode laser probe placement to accurately photoablate the equine ciliary body using a contact, transscleral approach. DESIGN Original research. PROCEDURES Forty-two freshly enucleated adult equine eyes were evaluated. The horizontal, medial vertical, central vertical, and lateral vertical lengths of the cornea were measured from limbus to limbus. Needles were inserted perpendicular to the sclera at specific distances posterior to the external limbus at the 1, 2, 4, 5, 6, 7, 8, 10, 11 and 12 o'clock positions. The per cent frequency that needles penetrated internal anatomical regions (lens, lens zonules, pars plicata, anterior, middle, and posterior pars plana, ora ciliaris retinae, or retina), when inserted at 2, 4, 6, and 8 mm posterior to the limbus, were calculated for all eyes combined, for right vs. left, for males vs. females, and for three age groups. The internal distance from the limbus to the anterior pars plana, and from the anterior pars plana to the ora ciliaris retinae were also measured in six of the horses (12 eyes). Mean distances and standard deviations were computed for all parameters. RESULTS Average corneal sizes and standard deviations were: 30.24 mm +/- 1.53 (horizontal); 24.69 mm +/- 1.52 (central vertical); 22.79 mm +/- 1.49 (medial vertical); and 19.79 mm +/- 1.55 (lateral vertical). Internal distances of the pars plicatas ranged from 5.33 mm +/- 0.49 to 10.67 mm +/- 1.15. Internal distances of the pars planas ranged from 0.33 mm +/- 0.49 to 3.17 mm +/- 0.39. High probabilities of penetrating the pars plicata correspond to positions 4 mm posterior to the external limbus dorsotemporal 10, 11 o'clock (OD), and 1, 2 o'clock (OS), dorsonasal 1 o'clock (OD) and 11 o'clock (OS), and ventrotemporal 5-7 o'clock (OU). Low probabilities of penetrating the pars plicata correspond to positions ventronasal 4 o'clock (OD), 8 o'clock (OS) and dorsonasal 2 o'clock (OD), 10 o'clock (OS) at 4 and 6 mm posterior to the external limbus as well as ventrotemporal 4 o'clock (OS), 8 o'clock (OD) at 6 mm posterior to the external limbus. CONCLUSIONS Transscleral cyclophotocoagulation may be a viable alternative to medical therapy for control of intraocular pressure in horses with glaucoma. Overall, the most accurate anatomical position on the sclera for cyclophotocoagulation of the equine eye is 4-6 mm posterior to the limbus, avoiding the nasal quadrants. Accurate transscleral cyclophotocoagulation should optimize the therapeutic outcome and minimize potential side-effects such as retinal detachment and cataract formation.
Grand challenge: real-time soccer analytics leveraging low-latency complex event processing

In this paper, we present a real-time capable event-based system, which is tailored towards analytical query processing in the context of soccer games. The main challenge is to meet the application's strict real-time and low-latency requirements in face of streams of high-velocity sensor data. We describe a workflow-like architecture for query processing based on a publish/subscribe model. Queries are structured into computational tasks that are arranged sequentially and/or in parallel. Tasks are connected by preallocated ring buffers providing total event ordering and fast as well as decoupled event access. Our evaluation results show the effectiveness of the proposed system in terms of low-latency processing under real-time conditions. Speeding up the system by a factor of 50 compared to real-time introduces almost no latency overhead.
Associations between Burkitt Lymphoma among Children in Malawi and Infection with HIV, EBV and Malaria: Results from a Case-Control Study

Background Burkitt lymphoma, a childhood cancer common in parts of sub-Saharan Africa, has been associated with Epstein Barr Virus (EBV) and malaria, but its association with human immunodeficiency virus (HIV) is not clear. Methodology/Principal Findings We conducted a case-control study of Burkitt lymphoma among children (aged ≤15 years) admitted to the pediatric oncology unit in Blantyre, Malawi between July 2005 and July 2006. Cases were 148 children diagnosed with Burkitt lymphoma and controls were 104 children admitted with non-malignant conditions or cancers other than hematological malignancies and Kaposi sarcoma. Interviews were conducted and serological samples tested for antibodies against HIV, EBV and malaria. Odds ratios for Burkitt lymphoma were estimated using unconditional logistic regression adjusting for sex, age, and residential district. Cases had a mean age of 7.1 years and 60% were male. Cases were more likely than controls to be HIV positive (Odds ratio (OR)) = 12.4, 95% Confidence Interval (CI) 1.3 to 116.2, p = 0.03). ORs for Burkitt lymphoma increased with increasing antibody titers against EBV (p = 0.001) and malaria (p = 0.01). Among HIV negative participants, cases were thirteen times more likely than controls to have raised levels of both EBV and malaria antibodies (OR = 13.2; 95% CI 3.8 to 46.6; p = 0.001). Reported use of mosquito nets was associated with a lower risk of Burkitt lymphoma (OR = 0.2, 95% CI, 0.03 to 0.9, p = 0.04). Conclusions Our findings support prior evidence that EBV and malaria act jointly in the pathogenesis of Burkitt lymphoma, suggesting that malaria prevention may decrease the risk of Burkitt lymphoma. HIV may also play a role in the etiology of this childhood tumor.
Nonlinear control of constrained linear systems via predictive reference management

A method based on conceptual tools of predictive control is described for solving set-point tracking problems wherein pointwise-in-time input and/or state inequality constraints are present. It consists of adding to a primal compensated system a nonlinear device, called command governor (CG), whose action is based on the current state, set-point, and prescribed constraints. The CG selects at any time a virtual sequence among a family of linearly parameterized command sequences, by solving a convex constrained quadratic optimization problem, and feeds the primal system according to a receding horizon control philosophy. The overall system is proved to fulfill the constraints, be asymptotically stable, and exhibit an offset-free tracking behavior, provided that an admissibility condition on the initial state is satisfied. Though the CG can be tailored for the application at hand by appropriately choosing the available design knobs, the required online computational load for the usual case of affine constraints is well tempered by the related relatively simple convex quadratic programming problem.
The Attentional Control of Reading: Insights from Behavior, Imaging and Development

Attentional control is important both for learning to read and for performing difficult reading tasks. A previous study invoked two mechanisms to explain reaction time (RT) differences between reading tasks with variable attentional demands (Balota et al. 2000). The present study combined behavioral and neuroimaging measures to test the hypotheses that there are two mechanisms of interaction between attentional control and reading; that these mechanisms are dissociable both behaviorally and neuroanatomically; and that the two mechanisms involve functionally separable control systems. First, RT evidence was found in support of the two-mechanism model, corroborating the previous study (Balota et al. 2000). Next, two sets of brain regions were identified as showing fMRI BOLD activity that maps onto the two-mechanism distinction. One set included bilateral Cingulo-opercular regions and mostly right-lateralized Dorsal Attention regions (CO/DA+). This CO/DA+ region set showed response properties consistent with a role in reporting which processing pathway (phonological or lexical) was biased for a particular trial. A second set was composed primarily of left-lateralized Frontalparietal (LFP) regions. Its signal properties were consistent with a role in response checking. These results suggest that subcomponents of attentional control interact with subcomponents of reading processes in healthy young adults.
Learning to Connect Language and Perception

To truly understand language, an intelligent system must be able to connect words, phrases, and sentences to its perception of objects and events in the world. Current natural language processing and computer vision systems make extensive use of machine learning to acquire the probabilistic knowledge needed to comprehend linguistic and visual input. However, to date, there has been relatively little work on learning the relationships between the two modalities. In this talk, I will review some of the existing work on learning to connect language and perception, discuss important directions for future research in this area, and argue that the time is now ripe to make a concerted effort to address this important, integrative AI problem.
Arterial Destiffening With Weight Loss in Overweight and Obese Middle-Aged and Older Adults

We tested the hypothesis that weight loss via a hypocaloric diet would reduce arterial stiffness in overweight and obese middle-aged and older adults. Thirty-six individuals were randomly assigned to a weight loss (n=25; age: 61.2±0.8 years; body mass index: 30.0±0.6 kg/m2) or a control (n=11; age: 66.1±1.9 years; body mass index: 31.8±1.4 kg/m2) group. Arterial stiffness was measured via carotid artery ultrasonography combined with applanation tonometry and carotid-femoral pulse wave velocity via applanation tonometry at baseline and after the 12-week intervention. Body weight, body fat, abdominal adiposity, blood pressure, &bgr;-stiffness index, and carotid-femoral pulse wave velocity were similar in the 2 groups at baseline (all P>0.05). Body weight (−7.1±0.7 versus −0.7±1.1 kg), body fat, and abdominal adiposity decreased in the weight loss group but not in the control group (all P<0.05). Brachial systolic and diastolic blood pressures declined (P<0.05) only in the weight loss group. Central systolic and pulse pressures did not change significantly in either group. &bgr;-Stiffness index (−1.24±0.22 versus 0.52±0.37 U) and carotid-femoral pulse wave velocity (−187±29 versus 15±42 cm/s) decreased in the weight loss group but not in the control group (all P<0.05). The reductions in carotid-femoral pulse wave velocity were correlated with reductions in total body and abdominal adiposity (r=0.357–0.602; all P<0.05). However, neither total body nor abdominal adiposity independently predicted reductions in arterial stiffness indices. In summary, our findings indicate that weight loss reduces arterial stiffness in overweight/obese middle-aged and older adults, and the magnitudes of these improvements are related to the loss of total and abdominal adiposity.
Joint phenotypes, evolutionary conflict and the fundamental theorem of natural selection

Multiple organisms can sometimes affect a common phenotype. For example, the portion of a leaf eaten by an insect is a joint phenotype of the plant and insect and the amount of food obtained by an offspring can be a joint trait with its mother. Here, I describe the evolution of joint phenotypes in quantitative genetic terms. A joint phenotype for multiple species evolves as the sum of additive genetic variances in each species, weighted by the selection on each species. Selective conflict between the interactants occurs when selection takes opposite signs on the joint phenotype. The mean fitness of a population changes not just through its own genetic variance but also through the genetic variance for its fitness that resides in other species, an update of Fisher's fundamental theorem of natural selection. Some similar results, using inclusive fitness, apply to within-species interactions. The models provide a framework for understanding evolutionary conflicts at all levels.
Glycerol Monolaurate Inhibits Lipase Production by Clinical Ocular Isolates Without Affecting Bacterial Cell Viability.

PURPOSE We sought to determine the relative lipase production of a range of ocular bacterial isolates and to assess the efficacy of glycerol monolaurate (GML) in inhibiting this lipase production in high lipase-producing bacteria without affecting bacterial cell growth. METHODS Staphylococcus aureus,Staphylococcus epidermidis,Propionibacterium acnes, and Corynebacterium spp. were inoculated at a density of 10(6)/mL in varying concentrations of GML up to 25 μg/mL for 24 hours at 37 °C with constant shaking. Bacterial suspensions were centrifuged, bacterial cell density was determined, and production of bacterial lipase was quantified using a commercial lipase assay kit. RESULTS Staphylococcus spp. produced high levels of lipase activity compared with P. acnes and Corynebacterium spp. GML inhibited lipase production by Staphylococcal spp. in a dose-dependent manner, with S. epidermidis lipase production consistently more sensitive to GML than S. aureus. Glycerol monolaurate showed significant (P < 0.05) lipase inhibition above concentrations of 15 μg/mL in S. aureus and was not cytotoxic up to 25 μg/mL. For S. epidermidis, GML showed significant (P < 0.05) lipase inhibition above 7.5 μg/mL. CONCLUSIONS Lipase activity varied between species and between strains. Staphylococcal spp. produced higher lipase activity compared with P. acnes and Corynebacterium spp. Glycerol monolaurate inhibited lipase production by S. aureus and S. epidermidis at concentrations that did not adversely affect bacterial cell growth. GML can be used to inhibit ocular bacterial lipase production without proving detrimental to commensal bacteria viability.
Reframing HIV care: putting people at the centre of antiretroviral delivery

The delivery of HIV care in the initial rapid scale‐up of HIV care and treatment was based on existing clinic‐based models, which are common in highly resourced settings and largely undifferentiated for individual needs. A new framework for treatment based on variable intensities of care tailored to the specific needs of different groups of individuals across the cascade of care is proposed here. Service intensity is characterised by four delivery components: (i) types of services delivered, (ii) location of service delivery, (iii) provider of health services and (iv) frequency of health services. How these components are developed into a service delivery framework will vary across countries and populations, with the intention being to improve acceptability and care outcomes. The goal of getting more people on treatment before they become ill will necessitate innovative models of delivering both testing and care. As HIV programmes expand treatment eligibility, many people entering care will not be ‘patients’ but healthy, active and productive members of society . To take the framework to scale, it will be important to: (i) define which individuals can be served by an alternative delivery framework; (ii) strengthen health systems that support decentralisation, integration and task shifting; (iii) make the supply chain more robust; and (iv) invest in data systems for patient tracking and for programme monitoring and evaluation.
Spray cooling heat transfer — Test and CFD analysis

Spray cooling of high temperature surfaces subject to large internal heat generation is analyzed by computational fluid dynamics (CFD) methods to determine heat transfer coefficients and the micro-physical details of coolant droplet-heated surface interactions governed by evaporative processes. A high speed, high magnification digital camera (6000 frames/sec) is used to provide test data for micron scale spray droplet size distribution and droplet velocity from a spray nozzle for different supply pressures for HFE 7100 and water coolants. Droplet test data are then applied to construct FLOW-3D CFD models [1] of numerous translating spherical droplets impacting a heated surface with internal volume heat generation and the transient, free-surface fluid dynamics and heat transfer processes computed. Transient, expanding/collapsing, chaotic coolant vapor regions generated by evaporative processes during successive multiple droplet impacts on flat and roughened surfaces sustaining large heat fluxes (from 30 to 300 W/cm2) are generated from the CFD solutions and shown to reproduce qualitative phase transition features observed from test photography. A computer program is provided to calculate heat transfer coefficients for different combinations of coolant droplet size, droplet velocity, droplet spatial distribution in nozzle sprays, heat flux magnitude, evaporation temperature and coolant flow rate incorporating the thermophysical coolant and wall properties for both flat and surface roughness cases. CFD results for a wide variety of droplet sizes, translation velocities, magnitudes of heat flux for flat and surface roughness patterns, coolant flow rates, coolant types and prescribed wall surface temperatures are used to provide physical insights into best ways to achieve maximum spray cooling heat transfer coefficients and avoid surface flooding and dry spotting. Use of high speed photographic micro-details of droplet impingement and evaporation structures on heated walls is made to qualitatively substantiate the CFD methodology by comparison of computed to test observations.