Miracle-dz/UniFAR-FTUs huggingface dataset:
CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies

Myeloid dendritic cells (DCs) can capture HIV-1 via the receptor CD169/Siglec-1 that binds to the ganglioside, GM3, in the virus particle membrane. In turn, HIV-1 particles captured by CD169, an I-type lectin, whose expression on DCs is enhanced upon maturation with LPS, are protected from degradation in CD169+ virus-containing compartments (VCCs) and disseminated to CD4+ T cells, a mechanism of DC-mediated HIV-1 trans-infection. In this study, we describe the mechanism of VCC formation and its role in immune evasion mechanisms of HIV-1. We find HIV-1-induced formation of VCCs is restricted to myeloid cells, and that the cytoplasmic tail of CD169 is dispensable for HIV-1 trafficking and retention within VCCs and subsequent trans-infection to CD4+ T cells. Interestingly, introduction of a di-aromatic endocytic motif in the cytoplasmic tail of CD169 that results in endocytosis of HIV-1 particles, suppressed CD169-mediated HIV-1 trans-infection. Furthermore, super-resolution microscopy revealed close association of CD169 and HIV-1 particles in surface-accessible but deep plasma membrane invaginations. Intriguingly, HIV-1 particles in deep VCCs were inefficiently accessed by anti-gp120 broadly neutralizing antibodies, VRC01 and NIH45-46 G54W, and thus were less susceptible to neutralization. Our study suggests that HIV-1 capture by CD169 can provide virus evasion from both innate (phagocytosis) and adaptive immune responses.
‘Cognitive systemic dichotomization’ in public argumentation and controversies

We describe and analyze an important cognitive obstacle in inter- and intra-community ar- gumentation processes, which we propose to call 'Cognitive Systemic Dichotomization' (CSD). This social phenomenon consists in the collective use of shared cognitive patterns based upon dichotomous schemati- zation of knowledge, values, and affection. We discuss the formative role of CSD on a community's collec- tive cognition, identity, and public discourse, as well as the challenges it raises to reasoned argumentation, and how different approaches to argumentation undertake to face this obstacle to the reasonable debate of issues of public concern.
Processing grammatical gender of role nouns: Further evidence from eye movements

Two eye-tracking experiments investigated the effects of masculine versus feminine grammatical gender on the processing of role nouns and on establishing coreference relations. Participants read sentences with the basic structure My is a such as My brother is a singer in a band. Role nouns were either masculine or feminine. Kinship terms were lexically male or female and in this way specified referent gender, i.e., the sex of the person referred to. Experiment 1 tested a fully crossed design including items with an incorrect combination of lexically male kinship term and feminine role name. Experiment 2 tested only correct combinations of grammatical and lexical/referential gender to control for possible effects of the incorrect items of Experiment 1. In early stages of processing, feminine role nouns, but not masculine ones, were fixated longer when grammatical and referential gender were contradictory (Bruder male–Sängerin fem/brother–[female] singer). In later stages of sentence wrap-up there were longer fixations for sentences with masculine than for those with feminine role nouns. Results of both experiments indicate that, for feminine role nouns, cues to referent gender are integrated immediately, whereas a late integration obtains for masculine forms.
Curriculum: Forming and Reshaping the Vision of Physical Education in a High Need, Low Demand World of Schools

This paper highlights events and issues in the development of physical education as a school subject. From the origin of physical culture in the German and Swedish “Battle of the Gymnastics Systems” to the advent of the New Physical Education in 1927, physical education curriculum has been a contested terrain. This remains true today as physical educators must compete for school funding and other resources with highly valued subject areas. Unfortunately, serious contextual constraints continue to hamper the efforts of highly motivated, effective physical educators to teach physical education content in schools. Perhaps in the future, better opportunities can be found in other venues where physical education can one day be perceived as a high need, high demand priority for children and their families.
Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation.

BACKGROUND & AIMS The exact role Entamoeba histolytica cysteine proteases play in overcoming the colonic mucus barrier, as a prerequisite to epithelial cell disruption, is not known. Herein, we determined whether E histolytica trophozoites expressing the antisense transcript to cysteine protease 5 (EhCP5) could degrade colonic mucin and destroy epithelial cells. METHODS Cysteine protease-deficient amoebae were generated by antisense inhibition of EhCP5, and assayed for proteolytic activity against [(35)S]cysteine-labeled mucin from LS 174T, and HT-29F Cl.16E cells. Recombinant EhCP5 mucinase activity was also assessed. Disruption of an intact mucus barrier and epithelial cell invasion by amoebae were measured using high mucin producing LS 174T and HT-29 Cl.16E monolayers or Chinese hamster ovary (CHO) cells devoid of a mucus barrier. RESULTS Trophozoites with reduced cysteine protease activity were ineffective at degrading [(35)S]cysteine-labeled colonic mucin compared to wild-type amoebae by >60%. However, bioactive recombinant EhCP5 degraded >45% of purified native mucin, which was specifically inhibited by the cysteine proteinase (CP) inhibitor, E-64. Cysteine protease-deficient trophozoites could not overcome a protective intact mucus barrier and disrupt LS 174T or HT-29F Cl.16 cell monolayers; however, they readily adhere to and disrupt CHO monolayers devoid of a mucus barrier. CONCLUSIONS These findings unravel a central role for E histolytica CPs as key virulence factors in disrupting an intact mucus barrier in the pathogenesis of intestinal amoebiasis.
Citizens' Juries and Deliberative Democracy

In the face of widespread dissatisfaction with contemporary democratic practice, there has been a growing interest in theories of deliberative democracy. However theorists have often failed to sufficiently address the question of institutional design. This paper argues that recent experiments with citizens' juries should be of interest to deliberative democrats. The practice of citizens' juries is considered in light of three deliberative democratic criteria: inclusivity, deliberation and citizenship. It is argued that citizens' juries offer important insights into how democratic deliberation could be institutionalized in contemporary political decision-making processes.
Online Early-Late Fusion Based on Adaptive HMM for Sign Language Recognition

In sign language recognition (SLR) with multimodal data, a sign word can be represented by multiply features, for which there exist an intrinsic property and a mutually complementary relationship among them. To fully explore those relationships, we propose an online early-late fusion method based on the adaptive Hidden Markov Model (HMM). In terms of the intrinsic property, we discover that inherent latent change states of each sign are related not only to the number of key gestures and body poses but also to their translation relationships. We propose an adaptive HMM method to obtain the hidden state number of each sign by affinity propagation clustering. For the complementary relationship, we propose an online early-late fusion scheme. The early fusion (feature fusion) is dedicated to preserving useful information to achieve a better complementary score, while the late fusion (score fusion) uncovers the significance of those features and aggregates them in a weighting manner. Different from classical fusion methods, the fusion is query adaptive. For different queries, after feature selection (including the combined feature), the fusion weight is inversely proportional to the area under the curve of the normalized query score list for each selected feature. The whole fusion process is effective and efficient. Experiments verify the effectiveness on the signer-independent SLR with large vocabulary. Compared either on different dataset sizes or to different SLR models, our method demonstrates consistent and promising performance.
Quality, Cost, and Health: An Integrative Model

This article presents an integrative model of the relations among health status, quality of care, and resource expenditure. It defines medical care quality in terms of outcomes, measured as the expected improvements in health status attributable to care. The consideration of how quality so defined is affected by the unconstrained, efficient use of resources for care leads to the specification of the absolutist definition of quality. Consideration of the incidence of individual and external costs and benefits of care provides the basis for distinguishing further between the individualized definition of quality, which depends upon individual preferences and ability to pay, and the social definition of quality, which includes consideration of external benefits, full social costs, and preferences for the distribution of welfare. An additional distinction is made between clinical efficiency and production efficiency. This article examines in detail the implications, for the selection of optimal strategies of care, of the three definitions of quality (absolutist, individualized, and social) and the two types of efficiency (clinical and production).
Glutamate-like immunoreactivity in identified neuronal populations of insect nervous systems

Glutamate is considered to be the most likely transmitter candidate at excitatory synapses onto skeletal muscles of insects. We investigated the distribution of glutamate-like immunoreactivity (Glu-LI) in identified motor neurons of glutaraldehyde-fixed metathoracic ganglia of the locust in paraffin serial sections. The presumably glutamatergic fast and slow extensor tibiae motor neurons show Glu-LI, whereas other cells, including the GABAergic common inhibitory motor neurons and the cluster of octopaminergic dorsal unpaired median cells, show rather low levels of staining. Immunoreactivity of the fast extensor tibiae motor neuron is located in soma, neurites, axon, and the terminal arborizations. A double-labeling experiment on sections of the locust metathoracic ganglion showed that antisera against glutamate and GABA discriminate between the presumably glutamatergic and GABAergic motor neurons and that GABA-LI-positive neurons are low in Glu-LI. The results suggest that Glu-LI can be used as a marker for detecting potential glutamatergic neurons in insects under the present conditions. Application of the glutamate antiserum to sections of the honeybee brain revealed Glu-LI in motor neurons but also in certain interneurons. The most prominent populations of Glu-LI-positive cells were the monopolar cells and large ocellar interneurons, which are first-order interneurons of the visual and ocellar system. Several groups of descending interneurons also showed Glu-LI. The distributions of Glu-LI and GABA-LI are complementary in locust and bee ganglia. The high level of Glu-LI in certain interneuronal populations, as well as in identified glutamatergic motor neurons, suggests that insect central nervous systems may contain glutamatergic neuronal pathways.
Utility of salivagram in pulmonary aspiration in pediatric patients: comparison of salivagram and chest radiography.

OBJECTIVE The purpose of our study was to correlate the results of the radionuclide salivagram with the corresponding chest radiography findings on patients being evaluated for salivary aspiration to determine the utility of the salivagram. MATERIALS AND METHODS We identified 222 patients younger than 21 years who underwent salivagram and chest radiography within 3 months of each other. Salivagrams were blindly interpreted by two readers and chest radiographs were blindly interpreted by two other readers. The kappa coefficient with 95% CI was used to measure the level of interobserver agreement. Multivariate logistic regression was applied to determine whether age, sex, and neurologic diagnosis were predictors of a positive salivagram, with the odds ratio used to estimate association. RESULTS Interobserver agreement on salivagram interpretation was excellent (κ = 0.988; p < 0.0001; 95% CI, 0.968-1.000). Interobserver agreement on chest radiography interpretation was excellent (κ = 0.905; p < 0.0001; 95% CI, 0.845-0.965). The salivagram was positive for aspiration in 55 patients (25%). Chest radiography was positive in 54 patients (24%). When the interpretations of the salivagram (normal or abnormal) were compared with interpretations of the chest radiograph (normal or abnormal), there were 213 agreements and nine disagreements (intermethod agreement κ = 0.891; p < 0.0001; 95% CI, 0.831-0.952). Independent of age (p = 0.80) and sex (p = 0.31), patients with a neurologic diagnosis had odds of a positive salivagram 5.6 times higher than other diagnoses (odds ratio = 5.6; 95% CI, 2.5-13.1; p < 0.0001). CONCLUSION Infants with abnormal findings on salivagrams also had a high rate of abnormal findings on chest radiographs, which may indicate that some of the lung disease may be due to aspirated saliva. Salivagrams may be useful in children at risk of aspiration to identify those in whom intervention may help minimize the consequences of aspiration.
Jurors' Perception of Witnesses with Intellectual Disabilities and the Influence of Expert Evidence

Purpose The aim of this study was to assess mock-jurors' perceptions of the evidence of witnesses with intellectual disabilities either with or without expert evidence and in comparison with witnesses from the general population. Method Sixty participants read transcripts of a mock trial focusing on the testimony of an eyewitness. Participants were assigned to one of three groups. The first was told that the witness was a person from the general population. The second was told that the witness had mild learning disabilities. The third group was told that the witness had mild learning disabilities and was given expert evidence concerning his abilities. Results While mock-jurors perceive witnesses with learning disabilities to be fundamentally honest, they are reluctant to rely on the evidence provided by witnesses with learning disabilities. Expert evidence can go some way to ameliorating the negative perceptions of the reliability of witnesses with learning disabilities. Conclusions Expert evidence can provide jurors with a certain degree of insight and understanding of an individual witness with intellectual disabilities that potentially increases the likelihood of achieving justice.
XenoTrust: event-based distributed trust management

This paper describes XenoTrust, the trust management architecture used in the XenoServer Open Platform: a public infrastructure for wide-area computing, capable of hosting tasks that span the full spectrum of distributed paradigms. We suggest that using an event-based publish /subscribe methodology for the storage, retrieval and aggregation of reputation information can help exploiting asynchrony and simplicity, as well as improving scalability.
Area-Efficient ESD Clamp Circuit With a Capacitance-Boosting Technique to Minimize Standby Leakage Current

This paper presents a new RC-based power-rail electrostatic discharge (ESD) clamp circuit, which achieves ultra-low leakage current while maintaining low silicon utilization. A capacitance-boosting technique is used in conjunction with mathematical analysis of area utilization to determine the best set of parameters to achieve the smallest implementation area in silicon. The proposed power-rail ESD clamp circuit has been verified in a 65-nm general-purpose CMOS process, which achieves an ultra-low standby leakage current of 80 nA at 25 °C under 1-V bias, as well as ESD robustness of a 4-kV human body model and a 250-V machine model with a silicon area of only 45 μm × 17 μm.
Analysis of Thalassiosira pseudonana Silicon Transporters Indicates Distinct Regulatory Levels and Transport Activity through the Cell Cycle

ABSTRACT An analysis of the expression and activity of silicon transporters (SITs) was done on synchronously growing cultures of the diatom Thalassiosira pseudonana to provide insight into the role these proteins play in cellular silicon metabolism during the cell cycle. The first SIT-specific polyclonal peptide antibody was generated and used in the immunoblot analysis of whole-cell protein lysates to monitor SIT protein levels during synchronized progression through the cell cycle. Peaks in SIT protein levels correlated with active periods of silica incorporation into cell wall substructures. Quantitative real-time PCR on each of the three distinct SIT genes (TpSIT1, TpSIT2, and TpSIT3) showed that mRNA levels for the most highly expressed SIT genes peaked during the S phase of the cell cycle, a period prior to maximal silicon uptake and during which cell wall silicification does not occur. Variations in protein and mRNA levels did not correlate, suggesting that a significant regulatory step of SITs is at the translational or posttranslational level. Surge uptake rates also did not correlate with SIT protein levels, suggesting that SIT activity is internally controlled by the rate of silica incorporation. This is the first study to characterize SIT mRNA and protein expression and cellular uptake kinetics during the course of the cell cycle and cell wall synthesis, and it provides novel insight into SIT regulation.
A Note on Comments by Nettheim and Cazden

In his comments on Gabriel (1978), Nettheim has pointed out that choosing moderate values for the components of a musical stimulus does not mean that their effect has been eliminated. He further suggests that as musical stimuli arc essentially multivariate, their study requires multivariate methods. The experiment reported by Gabriel involved subjects rating the perceived agreement between stimuli representing Cooke's Basic Terms and verbal descriptions. All of the verbal descriptions were provided by Cooke but some were allocated to the Basic Terms he intended and some were allocated randomly (with a constraint). As Nettheim points out, all experimental designs have their drawbacks and the experiment we are discussing may be thought of as having been conducted at a cross-section along the variables which were given a constant value. The essential point is that, if Cooke were correct in his belief concerning the role of Basic Terms, this should be apparent at the values chosen, especially perhaps because none of these were so eccentric as to be suspected of having an overwhelming influence. If the synthesised Basic Terms did express purity or simplicity as suggested by Nettheim they would do so equally for each presentation of each basic term and in general would not affect the comparisons of agreement ratings.
A disciplinary perspective of competency-based training on the acquisition of employability skills

In the changing global economy, employability skills increasingly are the focus of vocational education and training institutions. This paper explores the effect of academic disciplines, students’ background characteristics and industry training on the acquisition of employability skills through competency-based training. A significant relationship emerges between academic disciplines and industry training on the acquisition of employability skills. Some partial evidence suggests an impact of pre-education on the acquisition of employability skills; however, no such relationship appeared between gender and the skill acquisition. The results demonstrate the need for academic disciplines to determine specific employability skills required for social and community practice, as a basis for enhancing the development of employability skills in training programmes.
A study to enhance medical students’ professional decision-making, using teaching interventions on common medications

Aim To create sustained improvements in medical students’ critical thinking skills through short teaching interventions in pharmacology. Method The ability to make professional decisions was assessed by providing year-4 medical students at a UK medical school with a novel medical scenario (antenatal pertussis vaccination). Forty-seven students in the 2012 cohort acted as a pretest group, answering a questionnaire on this novel scenario. To improve professional decision-making skills, 48 students from the 2013 cohort were introduced to three commonly used medications, through tutor-led 40-min teaching interventions, among six small groups using a structured presentation of evidence-based medicine and ethical considerations. Student members then volunteered to peer-teach on a further three medications. After a gap of 8 weeks, this cohort (post-test group) was assessed for professional decision-making skills using the pretest questionnaire, and differences in the 2-year groups analysed. Results Students enjoyed presenting on medications to their peers but had difficulty interpreting studies and discussing ethical dimensions; this was improved by contextualising information via patient scenarios. After 8 weeks, most students did not show enhanced clinical curiosity, a desire to understand evidence, or ethical questioning when presented with a novel medical scenario compared to the previous year group who had not had the intervention. Students expressed a high degree of trust in guidelines and expert tutors and felt that responsibility for their own actions lay with these bodies. Conclusion Short teaching interventions in pharmacology did not lead to sustained improvements in their critical thinking skills in enhancing professional practice. It appears that students require earlier and more frequent exposure to these skills in their medical training.
Intra and Interorganizational Learning Networks and the Implementation of Quality Improvement Initiatives: The Case of a Portuguese Teaching Hospital

Learning has an important position in the development of employees and their expertise. This article focuses on the role and utilization of intra and interorganizational formal and informal learning within different types of learning networks. Specifically, [the authors] investigate different types of networks (inter- or intraorganizational) and different types of learning (formal or informal) that can occur within such networks. [The] qualitative case study is based on 46 expert interviews involving 49 interviewees, through which [the authors] explore how formal and informal learning was used in the development and implementation of quality improvement initiatives at a large public teaching hospital in Portugal. [The] analysis suggests that formal and informal learning can take place within different types of learning networks that draw on internal resources as well as on the collaboration with external entities. The article argues that it is important for [human resource development] HRD managers, seeking to support organizational learning, to understand how different types of learning take place, and which features of learning networks support these processes.