Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial Imaging, Speciation, and Quantification of Selenium in the Hyperaccumulator Plants Astragalus bisulcatus and Stanleya pinnata1

Astragalus bisulcatus and Stanleya pinnata hyperaccumulate selenium (Se) up to 1% of plant dry weight. In the field, Se was mostly present in the young leaves and reproductive tissues of both hyperaccumulators. Microfocused scanning x-ray fluorescence mapping revealed that Se was hyperaccumulated in trichomes in young leaves of A. bisulcatus. None of 10 other elements tested were accumulated in trichomes. Micro x-ray absorption spectroscopy and liquid chromatography-mass spectrometry showed that Se in trichomes was present in the organic forms methylselenocysteine (MeSeCys; 53%) and γ-glutamyl-MeSeCys (47%). In the young leaf itself, there was 30% inorganic Se (selenate and selenite) in addition to 70% MeSeCys. In young S. pinnata leaves, Se was highly concentrated near the leaf edge and surface in globular structures that were shown by energy-dispersive x-ray microanalysis to be mainly in epidermal cells. Liquid chromatography-mass spectrometry revealed both MeSeCys (88%) and selenocystathionine (12%) inside leaf edges. In contrast, both the Se accumulator Brassica juncea and the nonaccumulator Arabidopsis thaliana accumulated Se in their leaf vascular tissues and mesophyll cells. Se in hyperaccumulators appears to be mobile in both the xylem and phloem because Se-treated S. pinnata was found to be highly toxic to phloem-feeding aphids, and MeSeCys was present in the vascular tissues of a S. pinnata young leaf petiole as well as in guttation fluid. The compartmentation of organic selenocompounds in specific storage areas in the plant periphery appears to be a unique property of Se hyperaccumulators. The high concentration of Se in the plant periphery may contribute to Se tolerance and may also serve as an elemental plant defense mechanism.
Measuring the Burden of Neglected Tropical Diseases: The Global Burden of Disease Framework

Reliable, comparable information about the main causes of disease and injury in populations, and how these are changing, is a critical input for debates about priorities in the health sector. Traditional sources of information about the descriptive epidemiology of diseases, injuries, and risk factors are generally incomplete, fragmented, and of uncertain reliability and comparability. The Global Burden of Disease (GBD) study has provided a conceptual and methodological framework to quantify and compare the health of populations using a summary measure of both mortality and disability, the disability-adjusted life year (DALY). This paper describes key features of the Global Burden of Disease analytic approach, which provides a standardized measurement framework to permit comparisons across diseases and injuries, as well as risk factors, and a systematic approach to the evaluation of data. The paper describes the evolution of the GBD, starting from the first study for the year 1990, summarizes the methodological improvements incorporated into GBD revisions for the years 2000–2004 carried out by the World Health Organization, and examines priorities and issues for the next major GBD study, funded by the Bill & Melinda Gates Foundation, and commencing in 2007. The paper presents an overview of summary results from the Global Burden of Disease study 2002, with a particular focus on the neglected tropical diseases, and also an overview of the comparative risk assessment for 26 global risk factors. Taken together, trypanosomiasis, Chagas disease, schistosomiasis, leishmaniasis, lymphatic filariasis, onchocerciasis, intestinal nematode infections, Japanese encephalitis, dengue, and leprosy accounted for an estimated 177,000 deaths worldwide in 2002, mostly in sub-Saharan Africa, and about 20 million DALYs, or 1.3% of the global burden of disease and injuries. Further research is currently underway to revise and update these estimates.
Relationships between Law and Religion in American Society

Law and religion in American society have much in common. Historically, the seeds of the republic came from a harvest in which the law had not been completely severed from theology. Although the processes of secularization which thereafter followed tended to separate law from morality and its religious roots, the events of the current century have caused men to question positivistic bases as critiques for the law and to reflect anew upon the law beyond the law.2
Plasma lipids and blood fluidity in patients with polygenic hypercholesterolaemia treated with fluvastatin.

The clinical benefit brought about by HMG-CoA reductase inhibitors (statins) may not entirely be due to their lipid-lowering effect. Further investigation is necessary in order to determine the significance of ancillary effects to the clinical benefit of statin treatment. We studied 27 polygenic hypercholesterolaemia (PHC) patients before and 3 and 6 months after fluvastatin treatment. A control group of 38 normal, sex and age matched, subjects were also studied. The following parameters were measured: haematimetry, serum lipids and general biochemistry, apo-A/B and lipoproteins, fibrinogen, blood filterability, red blood cell aggregation, blood and plasma viscosity. PHC patients showed lower blood filterability (16.00+/-0.99 vs 19.90+/-2.90 microl/s), higher plasma fibrinogen (274.8+/-41.5 vs 241.6+/-43.2 mg/dl), increased erythrocyte aggregation at low shear stress (8.10+/-1.15 vs 7.19+/-1.29) and increased plasma viscosity (1.26+/-0.06 vs 1.23+/-0.05 mPa.s). Notable lipid changes after 6 months fluvastatin treatment were not accompanied by measurable changes in the haemorheological alterations of the PHC patients.
Unique neuronal functions of cathepsin L and cathepsin B in secretory vesicles: biosynthesis of peptides in neurotransmission and neurodegenerative disease

Abstract Proteases are required for the production of peptide neurotransmitters and toxic peptides in neurodegenerative diseases. Unique roles of the cysteine proteases cathepsin L and cathepsin B in secretory vesicles for the production of biologically active peptides have been demonstrated in recent studies. Secretory vesicle cathepsin L participates in the proteolytic conversion of proenkephalin into the active enkephalin, an opioid peptide neurotransmitter that mediates pain relief. Moreover, recent findings provide evidence that cathepsin B in regulated secretory vesicles participates in the production of toxic β-amyloid peptides that are known to accumulate extracellularly in Alzheimer's disease brains. The neurobiological functions of cathepsins L and B demonstrate that these secretory vesicle cysteine proteases produce biologically active peptides. These results demonstrate newly identified roles for cathepsins L and B in neurosecretory vesicles in the production of biologically active peptides.
Second-generation Proximally Coated Titanium Femoral Component: Minimum 7-year Results

It is unclear whether resurfacing or fully porous-coated or proximally coated stems are the best femoral components for young patients. First-generation proximally coated titanium fiber-metal femoral components had a high rate of failure. We prospectively followed 56 patients (70 hips) whom we believed were at high risk for loosening of a cemented femoral component; all patients were implanted with one second-generation proximally coated titanium femoral component. Ten patients (11 hips) died and three patients (four hips) were lost to minimum followup. Three models of titanium fiber-metal acetabular component were implanted with screws. Patients were clinically evaluated using the Harris hip score. We used standardized radiographs to assess fixation status and osteolysis. Forty-four patients (55 hips) with a mean age of 45 years were followed for a minimum of 7 years (mean, 10.5 years; range, 7-15 years). No femoral component had been revised for any reason. Bone ingrowth (spot welding) was seen in 41 hips (75%) and proximal femoral osteolysis in only four hips. Reoperations for polyethylene wear were performed in seven patients (12.7%) and two patients had liner exchange for recurrent dislocation. Even with an articulating surface with considerable polyethylene wear debris, these second-generation proximally coated titanium fiber-metal femoral components had a survival rate of 100% at a mean 10.5-year followup.Level of Evidence: Level IV, therapeutic study. See the Guidelines for Authors for a complete description of levels of evidence.
CHLOROPHYLL STUDIES ON BARLEY MUTANTS.

Those species of plants in which there is considerable information on the genetics of chlorophyll deficiency furnish favorable material for a study of physiological control by the gene over the production of chlorophyll and related pigments. This study is a preliminary survey of the pigments of a number of genetically different chlorophyll mutant stocks in barley. Rabinowitch (8) has pointed out that apart from the isolated observation of Inman and Blakeslee (d), who reported a chlorophyll with a different absorption spectrum from ordinary chlorophyll in an X-rayed mutant of Datura, the chlorophyll of all higher land plants investigated has been found to consist of the two components? the blue-green chlorophyll a and the yellow-green chlorophyll b. However, W. H. Eyster (3) in 1924 reported the "common" occurrence of four types of pigment-deficient plants in maize. These plants were those which: (1) contained carotin, xanthophyll and chlorophyll a but lacked chlorophyll b; (2) contained carotin, xanthophyll and chlorophyll b but lacked chlorophyll a; (3) contained carotin, xanthophyll and chlorophyll a and b in varying amounts; and (4) contained carotin and xanthophyll but lacked both chlorophyll a and chlorophyll b. Eyster reports that in his studies plants which lack either chlorophyll a or chlorophyll b are unable to synthesize sufficient carbohydrates to grow to maturity. Except for this one report by Eyster, the only organisms in which a chlorophyll component has been found lacking are some of the algae, (2), (? ), (12), (13), (15), (16), (18). The descriptive term "chlorina" has been applied to certain pale green chlorophyll-deficient characters in barley. The color may vary from pale green to yellow green in different chlorinas. Several of those chlorophylldeficient chlorinas have been described by Robertson (10), Nilsson-Ehle (7) and others. Most of them, when studied in crosses with normal green plants, differ from the normal by one recessive gene. Robertson (9) described one chlorina, however, which is apparently maternally inherited. When this chlorina, Coast V, is used as the female parent and crossed with green, the Fi, F2 and F3 are all chlorinas. The reciprocal cross, using it as the male parent, results in normal F,, F2 and F3.
Effects of cadmium on the cuttlefish Sepia pharaonis’ arginine kinase: unfolding kinetics integrated with computational simulations

Arginine kinase is closely associated with adaptation to environmental stresses such as high salinity and heavy metal ion levels in marine invertebrates. In this study, the effects of Cd2+ on the cuttlefish Sepia pharaonis’ arginine kinase (SPAK) were investigated. SPAK was isolated from the muscles of S. pharaonis and upon further purification, showed a single band on SDS-PAGE. Cd2+ effectively inactivated SPAK, and the double-reciprocal kinetics indicated that Cd2+ induced non-competitive inhibition of arginine and ATP. Spectrofluorometry results showed that Cd2+ induced tertiary structure changes in SPAK with the exposure of hydrophobic surfaces that directly induced SPAK aggregation. The addition of osmolytes, glycine, and proline successfully blocked SPAK aggregation and restored the conformation and activity of SPAK. Molecular dynamics simulations involving SPAK and Cd2+ showed that Cd2+ partly blocks the entrance of ATP to the active site, and this result is consistent with the experimental results showing Cd2+-induced inactivation of SPAK. These results demonstrate the effect of Cd2+ on SPAK enzymatic function and unfolding, including aggregation and the protective effects of osmolytes on SPAK folding. This study provides concrete evidence of the toxicity of Cd2+ in the context of the metabolic enzyme SPAK, and it illustrates the toxic effects of heavy metals and detoxification mechanisms in cuttlefish.
Optimized Parameter Estimation for the LPM Local Positioning System

The LPM local positioning system is based on a bi-static frequency-modulated continuous-wave radar where the frequency ramps are generated by two different units of circuitry: one of higher quality in the base station (BS) and one optimized for low power consumption in the transponder (TP). This difference in circuitry causes a small chirp rate difference (CRD) between the ramp incline of the BS and that of the TP, which has previously been considered negligible. We show that it is imperative to take account of this CRD in the intermediate frequency signal. We derived a method based on a nonlinear least squares (NLS) estimation principle to estimate its value. The NLS method can further be used for fault detection and to mitigate the effect of multipath signals, which we validate with both simulated and measured data.
Multi-constrained joint transportation tasks by teams of autonomous mobile robots using a dynamical systems approach

We present a distributed leader-helper architecture for teams of two autonomous mobile robots that jointly transport large payloads while avoiding collisions with obstacles (either static or dynamic). The leader navigates to the goal destination and the helper is responsible for maintaining an appropriate distance (which is a function of the object's length) to the leader. Both robots share the responsibility of ensuring that the transported object does not collide with obstructions. No path needs to be given a priori to the robots nor to the payload. The team is able to perform its transportation task in unknown environments that can have corridors, corners and may change the layout online. The payload can be of different dimensions. The team is able to cope with abrupt/strong perturbations that challenge the team behavior during the execution of the task. These characteristics make this approach suitable to be deployed in warehouses or office-like environments. The motion of each robot is controlled by a time series asymptotically stable states, which is formalized using the attractor dynamics approach to behavior based robotics. The advantages are: (i) the overt behavior is smooth and stable; (ii) because the behavior is generated as a time sequence of attractor states, for the control variables, it contributes to the overall asymptotically stability of the system that makes it robust against perturbations. We present results of experiments in simulated environments and with real robots in real environments.
EasyText: an Operational NLG System

This paper introduces EasyText, a fully operational NLG system. This application processes numerical data (in tables) in order to generate specific analytical commentaries of these tables. We start by describing the context of this particular NLG application (communicative goal, user profiles, etc.). We then shortly present the theoretical background which underlies EasyText, before describing its implementation, realization and evaluation.
Optic Disc Measurements with the 78 Diopter Lens, Zeiss 4-Mirror Contact Lens and Computerized Image Analysing System

We evaluated the 78 diopter (D) lens and Zeiss 4-mirror lens for the measurement of vertical and horizontal optic disc diameters during slit-lamp biomicroscopy and compared the results with the measurements made with the computerized image analysing system (IMAGEnet 640, Topcon, Japan) in 30 eyes of 30 patients. The 78D lens and Zeiss 4-mirror lens measurements both correlated well with the computerized image analysis measurements (r=0.881 for vertical and r=0.895 for horizontal disc diameter measurements with the 78D lens and r=0.883 for vertical and r=0.891 for horizontal disc diameter measurements with the Zeiss 4-mirror lens). The 78D lens measurements overestimated vertical disc diameter by 5.3% and horizontal disc diameter by 4.4%. The Zeiss 4-mirror lens underestimated the vertical disc diameter by 2.4% and horizontal disc diameter by 2.2%. Thus either lens can be used for a quick estimation of the optic disc size, since the results correlate well with the computerized image analysis measurements.
Improving Recommendation Effectiveness: Adapting a Dialogue Strategy in Online Travel Planning

Conversational recommender systems support a structured human-computer interaction in order to assist online tourists in important online activities such as travel planning and dynamic packaging. In this paper we describe the effects and advantages of a novel recommendation methodology based on Machine Learning techniques. It allows conversational systems to autonomously improve an initial strategy in order to learn a new one that is more effective and efficient. We applied and tested our approach within a prototype of an online travel recommender system in collaboration with the Austrian Tourism portal (Austria.info). In this paper, we present the features of this technology and the results of the online evaluation. We show that the learned strategy adapts its actions to the served users, and deviates from a rigid initial strategy. More importantly, we show that the optimal strategy is able to assist online tourists in acquiring their goals more efficiently than the initial strategy. It can be used by the system designer to understand the limitations of an existing interaction design and guide him in the adoption of a new one that is capable to improve customer relationship, the usage of their web site, and the conversion rate of their online users.
Free Form Surface Skinning of 3D Curve Clouds for Conceptual Shape Design

In product design, designers often create a multitude of concept sketches as part of the ideation and exploration process. Transforming such sketches to 3D digital models requires special expertise due to a lack of intuitive Computer Aided Design (CAD) tools suitable for rapid modeling. Recent advances in sketch-based user interfaces and immersive environments have introduced novel curve design methods that facilitate the transformation of such sketches into 3D digital models. However, rapid surfacing of such data remains an open challenge. Based on the observation that a sparse network of curves is reasonably sufficient to convey the intended geometric shape, we propose a new method for creating approximate surfaces on curve clouds automatically. A notable property of our method is that it relieves many topological and geometric restrictions of 3D conventional networks such as the curves do not need to be connected to one another or gently drawn. Our method calculates a 3D guidance vector field in the space that the curve cloud appears. This guidance vector field helps drive a deformable closed surface onto the curves. During this deformation, surface smoothness is controlled through a set of surface smoothing and subdivision operations. The resulting surface can be further beautified by the user manually using selective surface modification and fairing operations. We demonstrate the effectiveness of our approach on several case examples. Our studies have shown that the proposed technique can be particularly useful for rapid visualization.
Intrinsic connections of macaque striate cortex: afferent and efferent connections of lamina 4C

We have studied the intrinsic organization of macaque striate cortex by tracing the pattern of horseradish peroxidase (HRP)-labeled axons and cell bodies produced by microinjections of HRP into single cortical laminae. Both anterograde and retrograde transport results were used to examine: (1) the pattern of projections from lamina 4C to the superficial layers; (2) the projection from lamina 4C to deeper cortical layers; and (3) the projections to lamina 4C from other cortical laminae. Laminae 4C alpha and 4C beta differ in their pattern of projections to the superficial layers of striate cortex. Axons from neurons in lamina 4C beta ascend through lamina 4B without giving off collaterals and terminate in lamina 4A and in the base of lamina 3. By contrast, axons from neurons in lamina 4C alpha terminate in lamina 4B and less densely in the 4A/3B region. The projection from lamina 4C beta to lamina 4A is particularly dense and is distributed in a patchy fashion immediately above each injection site. The projection from lamina 4C beta to lamina 3B appears less dense and more widespread; we estimate that individual 4C beta axons may spread laterally for more than 400 micron. Furthermore, the pattern of HRP-labeled cell bodies in lamina 4C beta following injections into laminae 4A and 3B provides evidence for a subdivision within 4C beta. These injections always produce a large number of labeled neurons in the upper part of lamina 4C beta, whereas the lower portion contains few labeled neurons that are located immediately under the center of the injection site. Both lamina 4C alpha and lamina 4C beta also contribute less dense projections to the deeper layers of cortex. Lamina 4C beta projects mainly to lamina 6, whereas lamina 4C alpha contributes axon terminals to both lamina 5A and lamina 6. Neurons in lamina 6 provide the bulk of the intracortical projections to lamina 4C. The axons of these neurons are fine in caliber and have a delicate side-spine morphology that is quite distinct from lateral geniculate axon arbors. Neurons in lamina 5A also project onto lamina 4C, but the projections of these neurons appear concentrated in lamina 4C alpha. These results confirm or refine many conclusions about intrinsic connections of striate cortex drawn from Golgi material and suggest new patterns of connections not suspected from previous work.
‘We’re Finns here, and Russians there’: A longitudinal study on ethnic identity construction in the context of ethnic migration

In this article, we examine how ethnic Finnish migrants construct their ethnic identities before and after migrating from Russia to Finland. We use a discursive psychological (DP) approach to analyse our longitudinal focus group data. In the analysis, we focus on the ways in which the participants use category labels, rhetorical devices, interpretative repertoires and other discursive resources in accounting for their identities. We also consider the social functions these identity constructions have over the course of migration. In the pre-migration data, participants mostly presented themselves as Finns. In the post-migration data, a larger variety of self-labels was used and the Finnish identity was explicitly problematized. The three main interpretative repertoires used when constructing identities both in Russia and in Finland were the biological repertoire, the repertoire of socialization and the repertoire of intergroup relations.