Miracle-dz/UniFAR-FTUs huggingface dataset:
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina

We obtained a monoclonal antibody (RL13) that identifies three integral membrane proteins specific to the nuclear envelope of rat liver, a major 75-kD polypeptide and two more minor components of 68 and 55 kD. Immunogold labeling of isolated nuclear envelopes demonstrates that these antigens are localized specifically to the inner nuclear membrane, and that the RL13 epitope occurs on the inner membrane's nucleoplasmic surface where the nuclear lamina is found. When nuclear envelopes are extracted with solutions containing nonionic detergent and high salt to solubilize nuclear membranes and pore complexes, most of these integral proteins remain associated with the insoluble lamina. Since the polypeptides recognized by RL13 are relatively abundant, they may function as lamina attachment sites in the inner nuclear membrane. Major cross-reacting antigens are found by immunoblotting and immunofluorescence microscopy in all rat cells examined. Therefore, these integral proteins are biochemical markers for the inner nuclear membrane and will be useful models for studying nuclear membrane biogenesis.
Diversity in Butane Monooxygenases among Butane-Grown Bacteria

ABSTRACT Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physiological level. The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O2 was required for butane degradation. (ii) 1-Butanol was produced during butane degradation. (iii) Acetylene inhibited both butane oxidation and 1-butanol production. The responses to the known monooxygenase inactivator, ethylene, and inhibitor, allyl thiourea (ATU), discriminated butane degradation among the three bacteria. Ethylene irreversibly inactivated butane oxidation by P. butanovora but not by M. vaccae or CF8. In contrast, butane oxidation by only CF8 was strongly inhibited by ATU. In all three strains of butane-grown bacteria, specific polypeptides were labeled in the presence of [14C]acetylene. The [14C]acetylene labeling patterns were different among the three bacteria. Exposure of lactate-grown CF8 and P. butanovora and glucose-grownM. vaccae to butane induced butane oxidation activity as well as the specific acetylene-binding polypeptides. Ammonia was oxidized by all three bacteria. P. butanovora oxidized ammonia to hydroxylamine, while CF8 and M. vaccae produced nitrite. All three bacteria oxidized ethylene to ethylene oxide. Methane oxidation was not detected by any of the bacteria. The results indicate the presence of three distinct butane monooxygenases in butane-grown P. butanovora, M. vaccae, and CF8.
Twenty million years of continuous deformation along the Karakorum fault, western Tibet: A thermochronological analysis

The role of the Karakorum fault zone (KFZ) is debated. South of 33°N, ongoing dextral‐oblique slip along the SW edge of the Gar basin exhumes metamorphic and magmatic rocks of the Ayilari range. Minerals have recorded a continuum of deformation from temperatures >600–400°C down to <250°C. The 40Ar/39Ar ages, the oldest being 21.2 ± 1.0 Ma, yield minimum estimates for the initiation of the KFZ. These are in agreement with the U‐Th/Pb ages constraining the onset of deformation at ≥25–22 Ma. Thermochronologic results show slow cooling for the period ∼21–14 Ma, followed by rapid cooling between ∼14 and 4 Ma. These data demonstrate that right‐lateral motion was in progress in the early Miocene and that shear continued at least until 4 Ma, pointing to ≥20 Ma of deformation along the fault. Greenschist facies deformation superimposed upon the medium‐ to high‐grade deformation marks a kinematic change from pure dextral to dextral‐normal motion associated with the onset of rapid cooling. At the regional scale, the coexistence of transtension in the Gar basin with transpression documented along the Pangong range farther north suggests another example of the “zipper tectonics” model developed along the Red River fault. The kinematic shift induced the rise of the Ayilari range starting at 16–12 Ma and the incision of major river courses. The Indus River might have become captive of the relief at this time. The river's 120 km of apparent offset implies dextral motion at a long‐term rate of ≥8.5 ± 1.5 mm yr−1.
The EpiCanvas infectious disease weather map: an interactive visual exploration of temporal and spatial correlations

Advances in surveillance science have supported public health agencies in tracking and responding to disease outbreaks. Increasingly, epidemiologists have been tasked with interpreting multiple streams of heterogeneous data arising from varied surveillance systems. As a result public health personnel have experienced an overload of plots and charts as information visualization techniques have not kept pace with the rapid expansion in data availability. This study sought to advance the science of public health surveillance data visualization by conceptualizing a visual paradigm that provides an 'epidemiological canvas' for detection, monitoring, exploration and discovery of regional infectious disease activity and developing a software prototype of an 'infectious disease weather map'. Design objectives were elucidated and the conceptual model was developed using cognitive task analysis with public health epidemiologists. The software prototype was pilot tested using retrospective data from a large, regional pediatric hospital, and gastrointestinal and respiratory disease outbreaks were re-created as a proof of concept.
SDIG: Toward Software-Defined IPsec Gateway

The current IPsec gateway integrates many functions of IPsec operation, tunnel management and forwarding decision, which makes the IPsec gateway complicated in maintenance and deployment. The problem of maintaining such devices prevents IPsec VPN from applying widely. The emergence of SDN provides an innovative way to decouple the control plane and data plane. In this paper, a Software-Defined IPsec Gateway (SDIG) is proposed to achieve net2net IPsec VPN. Different from the traditional IPsec gateway, the SDIG device serves as a data plane equipment that just concentrates on exchanging IKE packets and encrypting/decrypting IP packets. A global view of SDIG devices can be constructed in the SDN controller by collecting the status of all devices. Therefore the controller can manage and configure SDIG devices centrally, and simplify deployment complexity. Outbound IP packets for the SDIG device can be viewed as a trigger to control the establishment of IPsec tunnels. The SDIG device and the controller exchange information through a customized southbound protocol. The prototype system of SDIG is implemented, and the preliminary experimental results show that the method is feasible and effective.
Preoperative Wound Infiltration with Bupivacaine Reduces Early and Late Opioid Requirement After Hysterectomy

We conducted a randomized, double-blind trial to evaluate the early and late analgesic effect of preoperative wound infiltration with bupivacaine 0.25% (40 mL) compared to placebo (NaCl 0.9%, 40 mL) in patients undergoing major surgery. Forty-one patients scheduled for elective hysterectomy during general anesthesia were included. The pain management focused on pain prevention, including preoperative administration of nonsteroidal antiinflammatory drugs (NSAIDs), and peroperative administration of opioids. Postoperatively patients received buprenorphine and/or acetaminophen on demand. A significant difference between treatments was evident in the 3-day postoperative trial period. With identical pain scores in the two groups, the requested total amount of buprenorphine was greater in the placebo group (2.0 [0-5.1] mg) (median and [range]) than in the bupivacaine group (0.8 [0-2.8] mg) (P < 0.05). The demand for analgesics occurred earlier in those who received placebo (225 min) than in those who received bupivacaine (345 min), but did not reach the level of significance. In conclusion, preoperative wound infiltration with bupivacaine improved immediate and late postoperative pain management after hysterectomy compared to placebo. (Anesth Analg 1996;83:376-81)
New insights into the role of soluble guanylate cyclase in blood pressure regulation

Purpose of reviewNitric oxide (NO)-soluble guanylate cyclase (sGC)-dependent signaling mechanisms have a profound effect on the regulation of blood pressure (BP). In this review, we will discuss recent findings in the field that support the importance of sGC in the development of hypertension. Recent findingsThe importance of sGC in BP regulation was highlighted by studies using genetically modified animal models, chemical stimulators/activators and inhibitors of the NO/sGC signaling pathway, and genetic association studies in humans. Many studies further support the role of NO/sGC in vasodilation and vascular dysfunction, which is underscored by the early clinical success of synthetic sGC stimulators for the treatment of pulmonary hypertension. Recent work has uncovered more details about the structural basis of sGC activation, enabling the development of more potent and efficient modulators of sGC activity. Finally, the mechanisms involved in the modulation of sGC by signaling gases other than NO, as well as the influence of redox signaling on sGC, have been the subject of several interesting studies. SummarysGC is fast becoming an interesting therapeutic target for the treatment of vascular dysfunction and hypertension, with novel sGC stimulating/activating compounds as promising clinical treatment options.
Genome‐wide transcriptome analysis of Aspergillus fumigatus exposed to osmotic stress reveals regulators of osmotic and cell wall stresses that are SakAHOG1 and MpkC dependent

Invasive aspergillosis is predominantly caused by Aspergillus fumigatus, and adaptations to stresses experienced within the human host are a prerequisite for the survival and virulence strategies of the pathogen. The central signal transduction pathway operating during hyperosmotic stress is the high osmolarity glycerol mitogen‐activated protein kinase cascade. A. fumigatus MpkC and SakA, orthologues of the Saccharomyces cerevisiae Hog1p, constitute the primary regulator of the hyperosmotic stress response. We compared A. fumigatus wild‐type transcriptional response to osmotic stress with the ΔmpkC, ΔsakA, and ΔmpkC ΔsakA strains. Our results strongly indicate that MpkC and SakA have independent and collaborative functions during the transcriptional response to transient osmotic stress. We have identified and characterized null mutants for four A. fumigatus basic leucine zipper proteins transcription factors. The atfA and atfB have comparable expression levels with the wild‐type in ΔmpkC but are repressed in ΔsakA and ΔmpkC ΔsakA post‐osmotic stress. The atfC and atfD have reduced expression levels in all mutants post‐osmotic stress. The atfA‐D null mutants displayed several phenotypes related to osmotic, oxidative, and cell wall stresses. The ΔatfA and ΔatfB were shown to be avirulent and to have attenuated virulence, respectively, in both Galleria mellonella and a neutropenic murine model of invasive pulmonary aspergillosis.
Composite electric S-brane solutions with maximal number of branes and several scalar fields

A (n+1)-dimensional cosmological model with a set of scalar fields and antisymmetric (p+2)-form is considered. Some of scalar fields may have negative kinetic terms, i.e. they may describe ``phantom'' fields. For certain odd dimensions (D = 4m+1 = 5, 9, 13, .) and (p+2)-forms (p = 2m-1 = 1, 3, 5, .) and non-exceptional dilatonic coupling vector $\vec{\lambda}$ we obtain cosmological-type solutions to the field equations. These solutions are characterized by self-dual or anti-self-dual charge density forms Q (of rank 2m) and may describe the maximal set of branes (i.e. when all the branes have non-zero charge densities). Some properties of these solutions are considered, e.g. Kasner-like behavior, the existence of non-singular (e.g. bouncing) solutions and those with acceleration. The solutions with bouncing and acceleration take place when at least there is one ``phantom'' field in the model.
Mechanomyographic responses are not influenced by the innervation zone for the vastus medialis

Introduction: The purposes of this study were to: (1) compare the power output–related patterns of absolute and normalized mechanomyographic (MMG) amplitude and mean power frequency (MPF) signals across the innervation zone (IZ) during incremental cycle ergometry; and (2) examine the influence of the IZ on the mean MMG amplitude and MPF values. Methods: Ten men performed incremental cycle ergometry tests to exhaustion. Surface MMG signals were recorded simultaneously from accelerometer arrangements placed on the vastus medialis over the IZ and proximal and distal to the IZ. Results: The results of polynomial regression indicate that the best‐fit model for each site was consistent for each subject for MMG amplitude, but not for MMG MPF. Two‐way analyses of variance (ANOVAs) revealed no site × power output interaction (P > 0.05) for absolute and normalized MMG amplitude and MPF values. Conclusion: Our findings demonstrate that the IZ does not influence the MMG signal during dynamic exercise. Muscle Nerve 44: 424–431, 2011
Purely stochastic binary decisions in cell signaling models without underlying deterministic bistabilities

Detection of different extracellular stimuli leading to functionally distinct outcomes is ubiquitous in cell biology, and is often mediated by differential regulation of positive and negative feedback loops that are a part of the signaling network. In some instances, these cellular responses are stimulated by small numbers of molecules, and so stochastic effects could be important. Therefore, we studied the influence of stochastic fluctuations on a simple signaling model with dueling positive and negative feedback loops. The class of models we have studied is characterized by single deterministic steady states for all parameter values, but the stochastic response is bimodal; a behavior that is distinctly different from models studied in the context of gene regulation. For example, when positive and negative regulation is roughly balanced, a unique deterministic steady state with an intermediate value for the amount of a downstream signaling product is found. However, for small numbers of signaling molecules, stochastic effects result in a bimodal distribution for this quantity, with neither mode corresponding to the deterministic solution; i.e., cells are in “on” or “off” states, not in some intermediate state. For a large number of molecules, the stochastic solution converges to the mean-field result. When fluctuations are important, we find that signal output scales with control parameters “anomalously” compared with mean-field predictions. The necessary and sufficient conditions for the phenomenon we report are quite common. So, our findings are expected to be of broad relevance, and suggest that stochastic effects can enable binary cellular decisions.
Laboratory constraints on models of earthquake recurrence

In this study, rock friction “stick‐slip” experiments are used to develop constraints on models of earthquake recurrence. Constant rate loading of bare rock surfaces in high‐quality experiments produces stick‐slip recurrence that is periodic at least to second order. When the loading rate is varied, recurrence is approximately inversely proportional to loading rate. These laboratory events initiate due to a slip‐rate‐dependent process that also determines the size of the stress drop and, as a consequence, stress drop varies weakly but systematically with loading rate. This is especially evident in experiments where the loading rate is changed by orders of magnitude, as is thought to be the loading condition of naturally occurring, small repeating earthquakes driven by afterslip, or low‐frequency earthquakes loaded by episodic slip. The experimentally observed stress drops are well described by a logarithmic dependence on recurrence interval that can be cast as a nonlinear slip predictable model. The fault's rate dependence of strength is the key physical parameter. Additionally, even at constant loading rate the most reproducible laboratory recurrence is not exactly periodic, unlike existing friction recurrence models. We present example laboratory catalogs that document the variance and show that in large catalogs, even at constant loading rate, stress drop and recurrence covary systematically. The origin of this covariance is largely consistent with variability of the dependence of fault strength on slip rate. Laboratory catalogs show aspects of both slip and time predictability, and successive stress drops are strongly correlated indicating a “memory” of prior slip history that extends over at least one recurrence cycle.
The glucagon-like peptides.

I. Introduction II. History of the Incretin Concept: Discovery of Gastric Inhibitory Polypeptide III. Discovery of GLP-1 IV. Structures of GLPs and Family of Glucagon-Related Peptides V. Tissue Distribution of the Expression of GLPs A. Pancreatic α-cells B. Intestinal L cells C. Central nervous system VI. Proglucagon Biosynthesis A. Organization/structure of the proglucagon gene B. Regulation of glucagon gene expression C. Posttranslational processing of proglucagon VII. Regulation of GLP Secretion A. Overview B. Intracellular signals C. Carbohydrates D. Fats E. Proteins F. Endocrine G. Neural H. GLP-2 VIII. Metabolism of GLPs A. GLP-1 B. GLP-2 IX. Physiological Actions of GLPs A. Overview B. Pancreatic islets C. Counterregulatory actions of GLP-1 and leptin on β-cells D. Stomach E. Lung F. Brain G. Liver, skeletal muscle, and fat H. Pituitary, hypothalamus, and thyroid I. Cardiovascular system J. GLP-2 X. GLP Receptors A. Structure B. Signaling C. Distribution D. Regulation E. GLP-2 XI. Pathophysiology o.
General Drift Analysis with Tail Bounds

Drift analysis is one of the state-of-the-art techniques for the runtime analysis of randomized search heuristics (RSHs) such as evolutionary algorithms (EAs), simulated annealing etc. The vast majority of existing drift theorems yield bounds on the expected value of the hitting time for a target state, e.g., the set of optimal solutions, without making additional statements on the distribution of this time. We address this lack by providing a general drift theorem that includes bounds on the upper and lower tail of the hitting time distribution. The new tail bounds are applied to prove very precise sharp-concentration results on the running time of a simple EA on standard benchmark problems, including the class of general linear functions. Surprisingly, the probability of deviating by an $r$-factor in lower order terms of the expected time decreases exponentially with $r$ on all these problems. The usefulness of the theorem outside the theory of RSHs is demonstrated by deriving tail bounds on the number of cycles in random permutations. All these results handle a position-dependent (variable) drift that was not covered by previous drift theorems with tail bounds. Moreover, our theorem can be specialized into virtually all existing drift theorems with drift towards the target from the literature. Finally, user-friendly specializations of the general drift theorem are given.
Initiation of the flexirubin biosynthesis in Chitinophaga pinensis

Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non‐isoprenoid aryl‐polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from Chitinophaga pinensis encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia‐lyase and a 4‐coumarate‐CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of C. pinensis was characterized by a combination of feeding experiments, high‐performance liquid chromatography tandem mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry.
Assessment of VoIP quality over Internet backbones

As the Internet evolves into a ubiquitous communication infrastructure and provides various services including telephony, it has to stand up to the toll quality standards set by traditional telephone companies. Our objective is to assess to what extent today's Internet meets this expectation. Our assessment is based on delay and loss measurements taken over wide-area backbone networks, considers realistic VoIP scenarios and uses quality measures appropriate for voice. Our findings indicate that although voice services can be adequately provided by some ISPs, a significant number of paths lead to poor performance even for excellent VoIP end-systems. This makes a strong case for special handling of voice traffic on those paths. Even on the good paths, rare loss events can occasionally cause perceptible degradation of voice quality. Finally, the appropriate choice of the playout buffer scheme for each path was found to be of critical importance for the perceived quality.
Development of augmented forward collision warning system for Head-Up Display

In this paper, we present an augmented forward collision warning system for Head-Up Display (HUD). The convergence of HUD and Augmented Reality (AR) needs challenge and make innovative application in automobile industry. We focus on an advance of Forward Collision Warning System (FCWS) by the fusion of HUD and AR. The proposed system detects the frontal vehicles and pedestrians, assesses the imminent danger caused by the detected results, and alerts the augmented warnings to support safe driving practices.
Resetting of renal blood autoregulation during acute blood pressure reduction in hypertensive rats.

Decrease in systemic blood pressure, duration of pressure decrease, and change in the activity of the renin or the sympathetic nervous system may represent mechanisms involved in resetting the renal blood flow (RBF) autoregulation found in hypertensive rats. Autoregulation of RBF, plasma renin concentration (PRC), and the time needed for resetting to take place were studied in the nonclipped kidney before and after removal of the clipped kidney of two- kidney, one-clip (2K1C) hypertensive rats and before and after mechanical reduction of the renal arterial pressure (RAP) for 10 min in the spontaneously hypertensive rat (SHR) and in the nonclipped kidney of 2K1C hypertensive rats with and without renal denervation. Mean arterial pressure (MAP) fell from 147 to 107 mmHg 30 min after removal of the clipped kidney, and the lower pressure limit of RBF autoregulation decreased from 113 to 90 mmHg ( P < 0.01); PRC fell. Mechanical reductions of RAP from 161 to 120 mmHg in the nonclipped kidney for 10 min did not change RBF, but at 120 mmHg, the lower pressure limit of RBF autoregulation was reduced from 115 mmHg before pressure reduction to 96 mmHg afterwards ( P < 0.02). In SHR, similar pressure reduction for 10 min decreased the lower pressure limit of RBF autoregulation from 106 to 86 mmHg ( P < 0.01). PRC was unchanged in both models, and denervation did not change RBF autoregulation. When RAP was reduced below the lower pressure limit of RBF autoregulation, RBF decreased ∼20%; the lower pressure limit of RBF autoregulation remained unchanged. In normotensive Wistar-Kyoto rats, pressure reduction did not change the range of RBF autoregulation. These results indicate that acute normalization of the pressure range of RBF autoregulation in hypertensive rats is dependent on the degree of pressure reduction of RAP, whereas renal innervation and PRC do not play a major role. We propose that the mechanism of resetting is due to afterstretch of noncontractile elements of the vessel wall or is caused by pure myogenic mechanisms. An effect of intrarenal angiotensin cannot be excluded.