Miracle-dz/UniFAR-FTUs huggingface dataset:
ANTIMICROBIAL AND ANTIOXIDANT ACIVITIES OF $OCIMUM~BASILICUM~VAR.~PURPUREUM$ ETHANOL EXTRACT

Many species of Ocimum genus have a long history in folk medicine and food industry. The aim of this study was to evaluate the antimicrobial and antioxidant activity of ethanol extract of leaves of Ocimum basilicum var. purpureum cultivated in Armenian (Ararat province, village Dvin, 1000–1200 m a.s.l.). Antimicrobial activity of O. basilicum leaves' extract was determined by agar disk-diffusion method. Different Gram-positive (Bacillus subtilis WT-A, Staphylococcus aureus MDC 5233, Enterococcus hirae ATCC 9790) and Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium MDC1754, ampicillin resistant E. coli dhpα-pUC18 and kanamycin resistant E. coli pARG25) bacteria and yeasts (Saccharomyces cerevisiae ATCC 9804 and S. cerevisiae ATCC 13007) were used as test-microorganisms. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was applied to measure the radical scavenging capacity of O. basilicum ethanol extract. The total flavonoid content in O. basilicum extract was determined employing AlCl3 colorimetric assay, and the value was 46.9±0.884 μg QE mg–1. Total phenolic content of studied extract was investigated by Folin–Ciocalteu assay. The content for O. basilicum extract was 317.75±4.105 μg of GAE mg–1. Antiradical activity of O. basilicum extract expressed as IC50 value of 19.37±0.38 µg·mL–1. Thus, O. bacilicum can be considered as potential source of biologically active substances.
COUPLING MATRIX OPTIMIZATION SYNTHESIS FOR FILTERS WITH CONSTANT AND FREQUENCY-VARIANT COUPLINGS

This paper presents a quickly converging optimization technique for synthesis of filters with constant and frequency-variant couplings (FVC). Unlike the works so far appeared in the literature, the proposed technique is not based on the direct optimization of scattering parameters with assigned topology, but it consists of two procedures. Firstly, an FVC coupling matrix with assigned topology is suitably transformed by means of scaling and rotations for obtaining the new coupling matrix with constant couplings. Then, the cost function is constructed as a least squares problem involving both the eigenvalues of the new coupling matrix with constant couplings and that of the transversal coupling matrix. The solution is found via the solvopt optimization method. Two numerical examples with different topologies and specifications are synthesized to show the validation of the method presented in this paper.
Synergistic Protection of Retinal Ganglion Cells (RGCs) by SARM1 Inactivation with CNTF in a Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy

We evaluated whether inhibiting sterile alpha and (Toll/interleukin receptor (TIR)) motif-containing 1 (SARM1) activity protects retinal ganglion cells (RGCs) following ischemic axonopathy (rodent nonarteritic anterior ischemic optic neuropathy: rNAION) by itself and combined with ciliary neurotrophic factor (CNTF). Genetically modified SARM1(−) rats were rNAION-induced in one eye and compared against equivalently induced wild-type animals of the same background. Optic nerve (ON) diameters were quantified using optical coherence tomography (SD-OCT). RGCs were quantified 30 d post-induction using retinal stereology for Brn3a(+) nuclei. ON sections were analyzed by TEM and immunohistochemistry. SARM1(−)(−) and WT animals were then bilaterally sequentially rNAION-induced. One eye received intravitreal vehicle injection following induction; the contralateral side received CNTF and was analyzed 30 d post-induction. Inhibiting SARM1 activity suppressed axonal collapse following ischemic axonopathy. SARM1(−) animals significantly reduced RGC loss, compared with WT animals (49.4 ± 6.8% RGC loss in SARM1(−) vs. 63.6 ± 3.2% sem RGC loss in WT; Mann–Whitney one-tailed U-test, (p = 0.049)). IVT-CNTF treatment vs. IVT-vehicle in SARM1(−) animals further reduced RGC loss by 24% at 30 d post-induction, but CNTF did not, by itself, improve long-term RGC survival in WT animals compared with vehicle (Mann–Whitney one-tailed t-test; p = 0.033). While inhibiting SARM1 activity is itself neuroprotective, combining SARM1 inhibition and CNTF treatment generated a long-term, synergistic neuroprotective effect in ischemic neuropathy. Combinatorial treatments for NAION utilizing independent neuroprotective mechanisms may thus provide a greater effect than individual treatment modalities.
Proteomic profiling of formalin-fixed paraffine-embedded tissue reveals key proteins related to lung dysfunction in idiopathic pulmonary fibrosis

Introduction Idiopathic pulmonary fibrosis (IPF) severely affects the lung leading to aberrant deposition of extracellular matrix and parenchymal stiffness with progressive functional derangement. The limited availability of fresh tissues represents one of the major limitations to study the molecular profiling of IPF lung tissue. The primary aim of this study was to explore the proteomic profiling yield of archived formalin-fixed paraffin-embedded (FFPE) specimens of IPF lung tissues. Methods We further determined the protein expression according to respiratory functional decline at the time of biopsy. The total proteins isolated from 11 FFPE samples of IPF patients compared to 3 FFPE samples from a non-fibrotic lung defined as controls, were subjected to label-free quantitative proteomic analysis by liquid chromatography-mass spectrometry (LC-MS/MS) and resulted in the detection of about 400 proteins. Results After the pairwise comparison between controls and IPF, functional enrichment analysis identified differentially expressed proteins that were involved in extracellular matrix signaling pathways, focal adhesion and transforming growth factor β (TGF-β) signaling pathways strongly associated with IPF onset and progression. Five proteins were significantly over- expressed in the lung of IPF patients with either advanced disease stage (Stage II) or impaired pulmonary function (FVC<75, DLCO<55) compared to controls; these were lymphocyte cytosolic protein 1 (LCP1), peroxiredoxin-2 (PRDX2), transgelin 2 (TAGLN2), lumican (LUM) and mimecan (OGN) that might play a key role in the fibrogenic processes. Discussion Our work showed that the analysis of FFPE samples was able to identify key proteins that might be crucial for the IPF pathogenesis. These proteins are correlated with lung carcinogenesis or involved in the immune landscape of lung cancer, thus making possible common mechanisms between lung carcinogenesis and fibrosis progression, two pathological conditions at risk for each other in the real life.
Towards Sustainable Tourism: An In-Depth Study Of Green Entrepreneurship Strategies In Indonesia

Sustainable tourism has emerged as a critical global concern in recent years, with increasing attention directed towards the environmental impact of the tourism industry. Indonesia, renowned for its natural beauty and cultural diversity, has witnessed a surge in tourism activities, accompanied by growing environmental challenges. This research paper presents a comprehensive qualitative analysis of green entrepreneurship initiatives within the Indonesian tourism sector. This study utilizes secondary data sources, primarily drawing from previous research journals, to investigate the strategies and practices employed by green entrepreneurs in the Indonesian tourism industry. The research explores the adoption of sustainable and environmentally friendly business models, innovations in eco-friendly technologies, and partnerships for conservation efforts. It also examines the challenges and opportunities faced by green entrepreneurs in their pursuit of sustainability. The findings of this research contribute to our understanding of the role of green entrepreneurship in mitigating the environmental impacts of tourism in Indonesia. By shedding light on successful strategies and identifying areas for improvement, this study aims to guide policymakers, entrepreneurs, and stakeholders toward more sustainable tourism practices. Ultimately, this research seeks to promote the preservation of Indonesia's rich natural and cultural heritage while fostering economic growth through responsible and environmentally conscious entrepreneurship.
The First Description of the Microbial Diversity in the Amarillo River (La Rioja, Argentina), a Natural Extreme Environment Where the Whole Microbial Community Paints the Landscape Yellow

The Amarillo River in Famatina, La Rioja, Argentina, is a natural acidic river with distinctive yellow-ochreous iron precipitates along its course. While mining activities have occurred in the area, the river’s natural acidity is influenced by environmental factors beyond mineralogy, where microbial species have a crucial role. Although iron-oxidising bacteria have been identified, a comprehensive analysis of the entire microbial community in this extreme environment has not yet been conducted. In this study, we employ high-throughput sequencing to explore the bacterial and fungal diversity in the Amarillo River and Cueva de Pérez terraces, considered prehistoric analogues of the current river basin. Fe(II)-enrichment cultures mimicking different environmental conditions of the river were also analysed to better understand the roles of prokaryotes and fungi in iron oxidation processes. Additionally, we investigate the ecological relationships between bacteria and fungi using co-occurrence and network analysis. Our findings reveal a diverse bacterial community in the river and terraces, including uncultured species affiliated with Acidimicrobiia, part of an uncharacterised universal microbial acidic diversity. Acidophiles such as Acidithiobacillus ferrivorans, the main iron oxidiser of the system, and Acidiphilium, which is unable to catalyse Fe(II) oxidation but has a great metabolic flexibility,, are part of the core of the microbial community, showing significant involvement in intraspecies interactions. Alicyclobacillus, which is the main Fe(II) oxidiser in the enrichment culture at 30 °C and is detected all over the system, highlights its flexibility towards the iron cycle. The prevalence of key microorganisms in both rivers and terraces implies their enduring contribution to the iron cycle as well as in shaping the iconic yellow landscape of the Amarillo River. In conclusion, this study enhances our understanding of microbial involvement in iron mineral precipitation, emphasising the collaborative efforts of bacteria and fungi as fundamental geological agents in the Amarillo River.
Utility of a Navigated High-Speed Drill in Robotic-Assisted Screw Placement for Spine Surgery

Purpose To elucidate the utility of a navigated high-speed drill used after the version upgrade in surgeries assisted by a spinal robotics system. Methods The subjects were 166 patients who underwent screw placement using a spinal robotics system between April 2021 to July 2023. A significant change during the study was the introduction of a navigated high-speed drill in 80 post-upgrade cases, aimed at improving drilling accuracy. Screw accuracy was analyzed using the Gertzbein and Robbins classification on postoperative CT scans. Screws placed before (pre-upgrade group: 718 screws in 86 cases) and after the system upgrade (post-upgrade group: 747 screws in 80 cases) were compared in terms of perfect accuracy and deviation rates. Results There were no significant differences in demographics or surgical details between the two groups. No significant differences were observed in the overall perfect accuracy rate and deviation rate (2.4% pre-upgrade vs. 2.0% post-upgrade) between the two groups. For the percutaneous pedicle screw (PPS), the perfect accuracy rate was significantly higher, and the deviation rate was significantly lower in the post-upgrade group (26.1% pre-upgrade vs. 4.4% post-upgrade). Notably, the post-upgrade group achieved 100% perfect accuracy and 0% deviation for the cortical bone trajectory screw (CBT) technique. Conclusions The introduction of the navigated high-speed drill did not significantly alter the overall perfect accuracy or deviation rates for robotic-assisted screw placement. However, its use did demonstrate improved outcomes in specific techniques such as PPS and CBT, indicating its potential value in addressing skiving in robotic-assisted minimally invasive surgeries.
An ecological characterization of the Stellwagen Bank National Marine Sanctuary region : oceanographic, biogeographic, and contaminants assessment

The mission of NOAA’s National Marine Sanctuary Program (NMSP) is to serve as the trustee for a system of marine protected areas, to conserve, protect, and enhance their biodiversity, ecological integrity, and cultural legacy while facilitating compatible uses. Since 1972, thirteen National Marine Sanctuaries, representing a wide variety of ocean environments, have been established, each with management goals tuned to their unique diversity. Extending from Cape Ann to Cape Cod across the mouth of Massachusetts Bay, Stellwagen Bank National Marine Sanctuary (NMS) encompasses 2,181 square kilometers of highly productive, diverse, and culturally unique Federal waters. As a result of its varied seafloor topography, oceanographic conditions, and high primary productivity, Stellwagen Bank NMS is utilized by diverse assemblages of seabirds, marine mammals, invertebrates, and fish species, as well as containing a number of maritime heritage resources. Furthermore, it is a region of cultural significance, highlighted by the recent discovery of several historic shipwrecks. Officially designated in 1992, Stellwagen Bank became the Nation’s twelfth National Marine Sanctuary in order to protect these and other unique biological, geological, oceanographic, and cultural features of the region. The Stellwagen Bank NMS is in the midst of its first management plan review since designation. The management plan review process, required by law, is designed to evaluate, enhance, and guide the development of future research efforts, education and outreach, and the management approaches used by Sanctuaries. Given the ecological and physical complexity of Stellwagen Bank NMS, burgeoning anthropogenic impacts to the region, and competing human and biological uses, the review process was challenged to assimilate and analyze the wealth of existing scientific knowledge in a framework which could enhance management decision-making. Unquestionably, the Gulf of Maine, Massachusetts Bay, and Stellwagen Bank-proper are extremely well studied systems, and in many regards, the scientific information available greatly exceeds that which is available for other Sanctuaries. However, the propensity of scientific information reinforces the need to utilize a comprehensive analytical approach to synthesize and explore linkages between disparate information on physical, biological, and chemical processes, while identifying topics needing further study. Given this requirement, a partnership was established between NOAA’s National Marine Sanctuary Program (NMSP) and the National Centers for Coastal Ocean Science (NCCOS) so as to leverage existing NOAA technical expertise to assist the Sanctuary in developing additional ecological assessment products which would benefit the management plan review process.
Impact of Coronavirus Disease 2019 (COVID-19) Pandemic on Surgical Site Infection in Patients with Inflammatory Bowel Disease—A Monocentric, Retrospective Cohort Study

(1) Background: Surgical site infections (SSIs) are a relevant problem with a 25% incidence rate after elective laparotomy due to inflammatory bowel disease (IBD). The aim of this study was to evaluate whether stricter hygienic measures during the COVID-19 pandemic influenced the rate of SSI. (2) Methods: This is a monocentric, retrospective cohort study comparing the rate of SSI in patients with bowel resection due to IBD during COVID-19 (1 March 2020–15 December 2021) to a cohort pre-COVID-19 (1 February 2015–25 May 2018). (3) Results: The rate of SSI in IBD patients with bowel resection was 25.8% during the COVID-19 pandemic compared to 31.8% pre-COVID-19 (OR 0.94; 95% CI 0.40–2.20; p = 0.881). There were seventeen (17.5%) superficial and four (4.1%) deep incisional and organ/space SSIs, respectively, during the COVID-19 pandemic (p = 0.216). There were more postoperative intra-abdominal abscesses during COVID-19 (7.2% vs. 0.9%; p = 0.021). The strictness of hygienic measures (mild, medium, strict) had no influence on the rate of SSI (p = 0.553). (4) Conclusions: Hygienic regulations in hospitals during COVID-19 did not significantly reduce the rate of SSI in patients with bowel resection due to IBD. A ban on surgery, whereby only emergency surgery was allowed, was likely to delay surgery and exacerbate the disease, which probably contributed to more SSIs and postoperative complications.
The Collection of Conidae Fleming, 1822 (Mollusca: Gastropoda) in Sarawak Natural History Museum

This study focuses on the taxonomic study of the gastropod’s family, Conidae, in the Sarawak Natural History Museum based on their collection of specimens. A total of 27 specimens were identified, encompassing 15 species, namely Conus virgo, Conus miles, Conus hyaena, Conus furvus, Conus eburneus, Conus crosnieri, Conus amadis, Conus marmoreus, Conus geographus, Conus guinaicus, Conus lividus, Conus ebraeus, Conus patricius, and two unidentified species. A dichotomous key is formed according to the specimens found in the museum. It is hoped that this study serves as a basis for further work on gastropods, especially the family Conidae in Sarawak.
Research on the Technological Progress of CZT Array Detectors

CdZnTe (CZT) is a new type of compound semiconductor that has emerged in recent years. Compared to other semiconductor materials, it possesses an ideal bandgap, high density, and high electron mobility, rendering it an excellent room-temperature composite semiconductor material for X-ray and γ-ray detectors. Due to the exceptional performance of CZT material, detectors manufactured using it exhibit high energy resolution, spatial resolution, and detection efficiency. They also have the advantage of operating at room temperature. CZT array detectors, furthermore, demonstrate outstanding spatial detection and three-dimensional imaging capabilities. Researchers worldwide have conducted extensive studies on this subject. This paper, building upon this foundation, provides a comprehensive analysis of CZT crystals and CZT array detectors and summarizes existing research to offer valuable insights for envisioning new detector methodologies.
Exploring the Untapped Potential of Neuromarketing in Online Learning: Implications and Challenges for the Higher Education Sector in Europe

This research investigates the impact of applying neuromarketing techniques to three practical examples of higher education (HE) branding: an official college website page, an official college Facebook page, and recorded online video lectures used for teaching at HE institutions. The study was conducted in three different HE institutions with a representative sample of 720 participants, with n = 529 used for testing the CARE college website, n = 59 for testing the HAZEF Facebook page, and n = 132 for testing the emotional response of students studying online. To assess the participants’ emotional responses, this study utilized automated facial coding through a webcam (15 Hz) and considered mood intensities. Additionally, a sentiment analysis was employed to verify the survey results and determine any discrepancies in the cognitive response. By analyzing gaze activity, movement patterns, and emotional responses, valuable insights were gained into students’ behaviors and preferences. This study recommends incorporating neuromarketing research into HE branding and online teaching to enhance students’ learning experiences. Overall, this study contributes to the understanding of human expectations and behaviors in response to online teaching and provides valuable insights for HE institutions in Europe.
The Development of Clinical Education Model Based on Community of Practice for the Patient Safety Competency Achievement in Nursing Students: a Pls-Sem Approach

Background: Patient safety goals competence has not been achieved optimally by nursing students. The efforts to solve this condition are through improving clinical education based on community of practice. The study aims to analyze the development of a clinical education model based on community of practice for the patient safety goals competency achievement in nursing students. Methods: This research method was a cross-sectional approach. The number of respondents were 125 nurses in the professional student program who were taken by a purposive sampling technique. The study assessed student factors, clinical educator factors, learning facilities factors, clinical education factors, and outcome factors (patient safety goals competency outcomes through using questionnaire. Datacollected from February to April 2023 in university hospital, Semarang City, Central Cava, Indonesia. Data analysis techniques in this study used the Structural Equation Modeling (SEM) of AMOS 5.0 softwares package. Results: Student factors, clinical educator factors, and learning facility factors have a significant effect on clinical education based on community of practice with p value <0.05, t statistic > 1.96, with path coefficient in the range of 0.251 -0.353. Student factors, clinical educator factors, learning facility factors, and community of practice-based clinical education have a significant effect on patient safety goals competencyy achievement with p value <0.05, t statistics > 1.96, and the path coefficient is in the range 0.135-0.372. Conclusions: The clinical education model based on the community of practice is formed by student factors, clinical educator factors, learning facility factors, and community of practice-based clinical education that directly influence the achievement of patient safety goals competency by nursing students. The clinical education model based on community of practice should be applied as a learning method in improving patient safety goals competency outcomes for nursing students in the clinical area.
Prevalence of diseases and its associated risk factors in crossbred Friesian calves

The aim of this study was to determine the prevalence of diseases and its associated risk factors in crossbred Friesian calves in a dairy farm in Bangladesh. Date of birth, birth weight, age and sex of 194 calves up to three months old, season of disease occurrence, date of illness and the percentage of IgG in colostrum were recorded. The prevalence of calf diseases was 33.5%, of which 30.1% were suffering from medical conditions, 19.6% having enteritis: only 5% had surgical disorders. Calves up to 21 days old had significantly higher disease prevalence (22.2%) than calves aged 22-90 days (11.3%). The prevalence of diseases was significantly higher (43.1%) in calves with birth weight up to 29 kg than calves with birth weight 30 kg or more (28.7%). The prevalence of diseases was higher in male calves (38.0%) than female (28.7%). The prevalence of diseases was significantly higher (72.4%) in calves consumed colostrum containing 22-24% IgG compared to calves given colostrum containing 28-30% IgG. The prevalence of diseases was significantly higher in rainy season (64.2%) than in summer (29.5%) and in winter (6.1%). Bang. vet. 2023. Vol. 40, No. 1 – 2, 37 – 45