Miracle-dz/UniFAR-FTUs huggingface dataset:
The Effect of Two Bulk Fill Resin Composites on Microleakage in Endodontically Treated Teeth

Purpose: To evaluate the effect of two bulk fill resin composites on microleakage in endodontically treated Class II restorations. Materials and Methods: Twenty-four non-carious molars were selected and randomly divided into three groups (n = 8). The Class II cavity preparations were made with the cervical margin 1 mm below the cementum-enamel junction, and endodontic treatment was performed using a resin-based sealer and gutta-percha points. In Group 1 (Control), resin composite (G-aenial Posterior; GC Corp., Tokyo, Japan) was applied to the interproximal wall, and then restorative material was applied incrementally. In Group 2, resin composite was applied to the interproximal wall, followed by a 4- mm-layer of a bulk fill flowable composite (x-tra base; Voco, GmbH, Cuxhaven, Germany) and the remaining occlusal part of the cavity was filled with resin composite. In Group 3, resin composite was applied to the interproximal wall, followed by a 4mm layer of a bulk fill fiber-reinforced composite (everX Posterior; GC Corp., Tokyo, Japan), and the remaining occlusal part of the cavity was filled with a resin composite. The restorations were then subjected to 500 thermal cycles, each with a dwell time of 20 seconds at 5 and 55 o C. The adaptation at the cervical margin was evaluated by dye penetration, and one tooth was used to evaluate the restorative material interface using SEM. The data were statistically analyzed using the Kruskal Wallis test (p < 0.05). Results: No significant difference in dye penetration was found between the control and the experimental groups. Microleakage was significantly higher on enamel margins compared with the dentin margins for all of the groups. Conclusion: The use of bulk fill restorative materials under resin composites does not affect the sealing ability of restorations.
Perovskite Crystals for Tunable White Light Emission

A significant fraction of global electricity demand is for lighting. Enabled by the realization and development of efficient GaN blue light-emitting diodes (LEDs), phosphor-based solid-state white LEDs provide a much higher efficiency alternative to incandescent and fluorescent lighting, which are being broadly implemented. However, a key challenge for this industry is to achieve the right photometric ranges and application-specific emission spectra via cost-effective means. Here, we synthesize organic–inorganic lead halide-based perovskite crystals with broad spectral tuneability. By tailoring the composition of methyl and octlyammonium cations in the colloidal synthesis, meso- to nanoscale 3D crystals (5–50 nm) can be formed with enhanced photoluminescence efficiency. By increasing the octlyammonium cations content, we observe platelet formation of 2D layered perovskite sheets; however, these platelets appear to be less emissive than the 3D crystals. We further manipulate the halide composition of the p.
Enhancing Academic Achievement And Retention In Senior Secondary School Chemistry Through Discussion And Lecture Methods: A Case Study Of Some Selected Secondary Schools In Gboko, Benue State, Nigeria

The present study was initiated to determine how academic achievement and retention in chemistry is enhanced using the two instructional methods among SSII students and ascertained the differential performance of male and female students in chemistry with a view of improving student performance in chemistry. The study adopted a non-equivalent pretest, posttest control quasi-experimental design. A total of one hundred and eighteen senior secondary school II students in intact classes were chosen from three schools in Gboko Local Government Area of Benue State using purposive random sampling. The ages of the students ranged between 16-17 years. Data were collected using a 30- item multiple choice Chemistry Achievement Test (CAT), in organic chemistry. Students were assigned to a treatment and a control group. Three hypotheses were generated and tested which were tested at 0.05 level of significance. The data obtained were analyzed using descriptive statistics, t-test, spearman’s correlation coefficient and analysis of variance (ANOVA). There was significant difference in students performance when discussion and lecture strategies were used to teach chemistry (F cal = 4.65 > F crit = 3.85 at P 0.2353 at P t crit = 2.000 at P < 0.05). The overall results showed that discussion instructional strategy significantly improved students’ performance in chemistry better than the lecture instructional strategy. The study concluded that discussion enhanced better achievement and productivity than the lecture method. It was recommended that discussion method should be use to teach organic chemistry in Nigerian Senior Secondary Schools.
Spinal Cord Stimulation for the Treatment of Failed Neck Surgery Syndrome: Outcome of a Prospective Case Series

Spinal cord stimulation (SCS) is an accepted, cost‐effective treatment option for a variety of chronic pain syndromes, including failed back surgery syndrome (FBSS). The application of SCS in the cervical spine, particularly for pain after cervical spine surgery, has been drawn into question in recent years by payers due to a purported lack of clinical evidence. To challenge this claim, we analyzed data from a prospective registry to support the use of SCS in the cervical spine for pain after spine surgery.
Tempol-mediated activation of latent iron regulatory protein activity prevents symptoms of neurodegenerative disease in IRP2 knockout mice

In mammals, two homologous cytosolic regulatory proteins, iron regulatory protein 1 (also known as IRP1 and Aco1) and iron regulatory protein 2 (also known as IRP2 and Ireb2), sense cytosolic iron levels and posttranscriptionally regulate iron metabolism genes, including transferrin receptor 1 (TfR1) and ferritin H and L subunits, by binding to iron-responsive elements (IREs) within target transcripts. Mice that lack IRP2 develop microcytic anemia and neurodegeneration associated with functional cellular iron depletion caused by low TfR1 and high ferritin expression. IRP1 knockout (IRP1−/−) animals do not significantly misregulate iron metabolism, partly because IRP1 is an iron-sulfur protein that functions mainly as a cytosolic aconitase in mammalian tissues and IRP2 activity increases to compensate for loss of the IRE binding form of IRP1. The neurodegenerative disease of IRP2−/− animals progresses slowly as the animals age. In this study, we fed IRP2−/− mice a diet supplemented with a stable nitroxide, Tempol, and showed that the progression of neuromuscular impairment was markedly attenuated. In cell lines derived from IRP2−/− animals, and in the cerebellum, brainstem, and forebrain of animals maintained on the Tempol diet, IRP1 was converted from a cytosolic aconitase to an IRE binding protein that stabilized the TfR1 transcript and repressed ferritin synthesis. We suggest that Tempol protected IRP2−/− mice by disassembling the cytosolic iron-sulfur cluster of IRP1 and activating IRE binding activity, which stabilized the TfR1 transcript, repressed ferritin synthesis, and partially restored normal cellular iron homeostasis in the brain.
p62/SQSTM1 Accumulation in Squamous Cell Carcinoma of Head and Neck Predicts Sensitivity to Phosphatidylinositol 3-Kinase Pathway Inhibitors

The phosphoinositol-3 kinase (PI3K) pathway is highly dysregulated in squamous cell carcinoma of the head and neck (SCCHN). While inhibitors of the PI3K/AKT pathway are being developed in cancer, their efficacy does not appear to be related to the presence of mutations or amplification in pathway genes. The PI3K pathway is a major regulator of macro-autophagy, an evolutionarily conserved catabolic process that degrades cellular materials to promote cellular homeostasis and survival under stress. Employing a panel of SCCHN cell lines, we observed a significant correlation between the activity of PI3K/AKT inhibitors and their ability to induce autophagy. More specifically, resistance to these inhibitors was associated with accumulation of p62/SQSTM1, a pleotropic protein that is consumed during autophagy, while loss of autophagy was, for the first time, found to be due to silencing of an essential autophagy gene, ATG7. Moreover, modulating ATG7 and p62/SQSTM1 could regulate sensitivity to PI3K/AKT inhibitors, underscoring a mechanistic link between autophagy and drug sensitivity. Analysis of human tissues revealed progressive accumulation of p62/SQSTM1 in a significant proportion of cancer samples compared to normal tissue, suggesting that defective autophagy has relevance to SCCHN. These findings are further validated by analysis of TCGA data confirming homozygous deletion and mRNA down-regulation of ATG7 in 10.0% of SCCHN samples. Taken together, these data indicate that p62/SQSTM1 levels modulate sensitivity to PI3K/AKT inhibitors; cancers vary in their capacity to undergo autophagy through epigenetic modification and, when deficient, accumulate p62/SQSTM1; and expression of autophagy-related proteins may serve as markers for resistance to PI3K/AKT inhibitors in SCCHN.
Achieving Sustained Virological Response in Liver Transplant Recipients With Hepatitis C Decreases Risk of Decline in Renal Function

The effect of antiviral therapy (AVT) on kidney function in liver transplantation (LT) recipients has not been well described despite known association of hepatitis C virus (HCV) infection with chronic kidney disease (CKD). We compared the incidence of CKD and end‐stage renal disease (ESRD) in 204 LT recipients with HCV based on treatment response to AVT. The mean estimated glomerular filtration rate (eGFR) at baseline (3 months after LT) was similar in the sustained virological response (SVR; n = 145) and non‐SVR group (n = 59; 69 ± 21 versus 65 ± 33 mL/minute/1.73 m2; P = 0.27). In the unadjusted Cox proportional regression analysis, the presence of SVR was associated with an 88% lower risk of CKD (hazard ratio, 0.12; 95% confidence interval [CI], 0.05‐0.31) and 86% lower risk of ESRD (odds ratio, 0.14; 95% CI, 0.05‐0.35). Similar results were found after adjusting for propensity score and time‐dependent Cox regression analyses. The estimated slopes of eGFR based on a 2‐stage mixed model of eGFR were calculated. Patients with SVR had a less steep slope in eGFR (–0.60 mL/minute/1.73 m2/year; 95% CI, –1.50 to 0.30; P = 0.190) than recipients without SVR (–2.53 mL/minute/1.73 m2/year; 95% CI, –3.99 to –1.07; P = 0.001), and the differences in the slopes were statistically significant (P = 0.026). In conclusion, in LT recipients with chronic HCV infection, achieving SVR significantly lowers the risk of decline in renal function and progression to ESRD independent of the AVT therapy used.
Comparison of a Virtual Game-Day Experience on Varying Devices

Collegiate athletics, particularly football, provide tremendous value to schools through branding, revenue, and publicity. As a result, extensive effort is put into recruiting talented students. When recruiting, home games are exceptional tools used to show a school's unique game-day atmosphere. However, this is not a viable option during the offseason or for off-site visits. This paper explores a solution to these challenges by using virtual reality (VR) to recreate the game-day experience. The Virtual Reality Application Center in conjunction with Iowa State University (ISU) athletics, created a VR application mimicking the game-day experience at ISU. This application was displayed using the world's highest resolution six-sided CAVETM, an Oculus Rift DK2 computer-driven head mounted display (HMD) and a Merge VR smart phone-driven HMD. A between-subjects user study compared presence between the different systems and a video control. In total, 82 students participated, indicating their presence using the Witmer and Singer questionnaire. Results revealed that while the CAVETM scored the highest in presence, the Oculus and Merge only experienced a slight drop compared to the CAVETM. This result suggests that the mobile ultra-low-cost Merge is a viable alternative to the CAVE TM and Oculus for delivering the game-day experience to ISU recruits.
Does the risk of hospitalisation for ischaemic heart disease rise already before widowhood?

Background The death of a spouse has been shown to increase mortality from various causes, including ischaemic heart disease. It is unclear, however, whether cardiac problems are already on the rise before widowhood. Methods Using longitudinal register data of Finnish widows-to-be aged 65 and over at baseline (N=19 185), we assessed the risk of hospitalisation for ischaemic heart disease 18 months before and after widowhood. Hospital admissions were derived from national hospital discharge registers between 1996 and 2002. Analyses used population-averaged and fixed-effects logistic models, the latter of which controlled for unobserved time-invariant characteristics, such as genetic susceptibility, personality and behavioural and medical history. Results For men, fixed-effects model revealed that hospitalisation for ischaemic heart disease increased twofold already 0–3 months prior to the death of a spouse (OR=2.09, 95% CI 1.22 to 3.60), relative to the period of 15–18 months before widowhood. It stayed at a heightened level up to 6 months following bereavement (OR=2.15, 95% CI 1.07 to 4.30). Among women, the fixed-effects analysis detected no statistically significant increase in hospitalisation for ischaemic heart disease before or after widowhood. Conclusions These findings indicate that men are already vulnerable to cardiac problems before the death of a wife. Medical interventions and health counselling could be targeted to the husbands of terminally ill patients, in order to improve their cardiovascular health over the transition to widowhood.
Social Value Orientations and Impressions of Partner's Honesty and Intelligence: A Test of the Might Versus Morality Effect

This research evaluates the might vs. morality effect (Liebrand, Jansen, Rijken, & Suhre, 1986) by examining whether the manipulation of the perception of partner's honesty and intelligence interacts with the observer's own social value orientation to influence the latter's expectations regarding partner cooperation and own cooperation in a social dilemma. Results reveal that greater cooperation was expected from an honest partner than from a dishonest partner and that this effect was stronger for prosocial Ss than for individualists and competitors. Conversely, individualists and competitors expected greater cooperation from an unintelligent partner than from an intelligent partner, whereas prosocial Ss did not expect differences between these partners. Similar findings were obtained for own cooperation, although social value orientations did not interact with partner intelligence. Considerable research has been focused on how individuals form, maintain, and organize personality impressions, revealing that people readily form impressions of others, that such impressions are fairly stable, and that such impressions frequently are organized along dimensions of social meaning, such as honesty and intelligence (e.g., for theoretical analyses, see Reeder & Brewer, 1979; Rosenberg SSkowronski
1,6-Hexanedithiol Monolayers on Au(111): a multitechnique structural study

Monolayers of 1,6-hexanedithiol [HS(CH2)6SH] deposited on Au(111) from the gas phase were characterized by scanning tunneling microscopy (STM), grazing incidence X-ray diffraction (GIXD), and low-energy atom diffraction (LEAD). Molecular resolution STM images suggest that the molecules lie prone in a striped arrangement with an inter-row spacing of 5 A. For the films prepared at an elevated temperature, two uniaxial incommensurate phases were found by GIXD. With respect to the surface substrate net, the diffraction patterns of both phases can be described by rectangular (p × √3) nets, where p is 4.24 ± 0.01 and 4.30 ± 0.01. These values of p correspond to spacings of 12.23 ± 0.04 and 12.40 ± 0.02 A along the nearest-neighbor (NN) direction of the substrate, whereas the spacing along the next-nearest-neighbor direction is 5 A in both cases. The LEAD patterns can be described by a 3 × 1 superlattice with respect to the mesh observed by GIXD. Lattice nonuniformity and angular broadening along the NN directio.
Establishment and Characterization of a Novel Caco-2 Subclone with a Similar Low Expression Level of Human Carboxylesterase 1 to Human Small Intestine

Caco-2 cells predominantly express human carboxylesterase 1 (hCE1), unlike the human intestine that predominantly expresses human carboxylesterase 2 (hCE2). Transport experiments using Caco-2 cell monolayers often lead to misestimation of the intestinal absorption of prodrugs because of this difference, as prodrugs designed to increase the bioavailability of parent drugs are made to be resistant to hCE2 in the intestine, so that they can be hydrolyzed by hCE1 in the liver. In the present study, we tried to establish a new Caco-2 subclone, with a similar pattern of carboxylase expression to human intestine, to enable a more accurate estimation of the intestinal absorption of prodrugs. Although no subclone could be identified with high expression levels of only hCE2, two subclones, #45 and #78, with extremely low expression levels of hCE1 were subcloned from parental Caco-2 cells by the limiting dilution technique. Unfortunately, subclone #45 did not form enterocyte-like cell monolayers due to low expression of claudins and β-actin. However, subclone #78 formed polarized cell monolayers over 4 weeks and showed similar paracellular and transcellular transport properties to parental Caco-2 cell monolayers. In addition, the intestinal transport of oseltamivir, a hCE1 substrate, could be evaluated in subclone #78 cell monolayers, including P-glycoprotein–mediated efflux under nonhydrolysis conditions, unlike parental Caco-2 cells. Consequently, it is proposed that subclone #78 may provide a more effective system in which to evaluate the intestinal absorption of prodrugs that are intended to be hydrolyzed by hCE1.
High‐functioning pervasive developmental disorders in adults

High‐functioning pervasive developmental disorders (PDDs) have only recently been widely recognised; they are diagnosed mainly in children. Key features are impaired social cognition and communication; obsessive interests, routines or activities; and social or occupational dysfunction. There are scant data about the prevalence of high‐functioning PDDs in adults, and it is possible that many Australian adults with these conditions are undiagnosed. A specialist multidisciplinary approach is used for both children with PDDs and adults with other neuropsychiatric disabilities, and has the potential to help adults with high‐functioning PDDs. Increased awareness and diagnosis of these conditions should not limit career or personal goals of individuals with PDDs but should aid them in finding happy and productive careers and lives.
Teratogenic Activity of Nitrous Oxide

Exposure of pregnant rats to an atmosphere containing nitrous oxide resulted in foetuses with abnormalities of vertebrae and ribs, and in death and resorption of foetuses. The lethal effects seemed to be selective, for surviving foetuses showed a smaller male/female sex ratio than did the controls.
Antibacterial activity of propolis collected in different regions of Brazil

The aim of this study was to investigate the antibacterial activity of propolis samples from Goias, Parana and Sao Paulo States, Brazil, and their flavonoids content. Ethanolic extracts of propolis (EEP) were prepared (30g of propolis in 70% ethanol), and the microorganisms Staphylococcus aureus and Escherichia coli were tested. The methodology employed was agar diffusion using filter paper discs. Ampicillin and tetracycline were used as controls. Antibacterial activity was determined by the reading of inhibition zone diameters (mm) after 24 hours incubation at 37°C. Results demonstrated that EEP inhibited the growth of Staphylococcus aureus but not that of Escherichia coli. Tetracycline and ampicillin showed an efficient action against both bacteria. Flavonoids content was variable, depending on the propolis sample. According to the results, it may be concluded that EEP showed effective action against Gram-positive bacteria, independently on their geographic origin, and a positive correlation between antibacterial activity and flavonoids content.
Atenolol Induced HDL-C Change in the Pharmacogenomic Evaluation of Antihypertensive Responses (PEAR) Study

We sought to identify novel pharmacogenomic markers for HDL-C response to atenolol in participants with mild to moderate hypertension. We genotyped 768 hypertensive participants from the Pharmacogenomic Evaluation of Antihypertensive Responses (PEAR) study on the Illumina HumanCVD Beadchip. During PEAR, participants were randomized to receive atenolol or hydrochlorothiazide. Blood pressure and cholesterol levels were evaluated at baseline and after treatment. This study focused on participants treated with atenolol monotherapy. Association with atenolol induced HDL-C change was evaluated in 232 whites and 152 African Americans using linear regression. No SNPs achieved a Bonferroni corrected P-value. However, we identified 13 regions with consistent association across whites and African Americans. The most interesting of these regions were seven with prior associations with HDL-C, other metabolic traits, or functional implications in the lipid pathway: GALNT2, FTO, ABCB1, LRP5, STARD3NL, ESR1, and LIPC. Examples are rs2144300 in GALNT2 in whites (P=2.29x10-4, β=-1.85 mg/dL) and rs12595985 in FTO in African Americans (P=2.90x10-4, β=4.52 mg/dL), both with consistent regional association (P<0.05) in the other race group. Additionally, baseline GALNT2 expression differed by rs2144300 genotype in whites (P=0.0279). In conclusion, we identified multiple gene regions associated with atenolol induced HDL-C change that were consistent across race groups, several with functional implications or prior associations with HDL-C.
Molecular and Cellular Features of Murine Craniofacial and Trunk Neural Crest Cells as Stem Cell-Like Cells

The outstanding differentiation capacities and easier access from adult tissues, cells derived from neural crest cells (NCCs) have fascinated scientists in developmental biology and regenerative medicine. Differentiation potentials of NCCs are known to depend on their originating regions. Here, we report differential molecular features between craniofacial (cNCCs) and trunk (tNCCs) NCCs by analyzing transcription profiles and sphere forming assays of NCCs from P0-Cre/floxed-EGFP mouse embryos. We identified up-regulation of genes linked to carcinogenesis in cNCCs that were not previously reported to be related to NCCs, which was considered to be, an interesting feature in regard with carcinogenic potentials of NCCs such as melanoma and neuroblastoma. Wnt signal related genes were statistically up-regulated in cNCCs, also suggesting potential involvement of cNCCs in carcinogenesis. We also noticed intense expression of mesenchymal and neuronal markers in cNCCs and tNCCs, respectively. Consistent results were obtained from in vitro sphere-forming and differentiation assays. These results were in accordance with previous notion about differential potentials of cNCCs and tNCCs. We thus propose that sorting NCCs from P0-Cre/floxed-EGFP mice might be useful for the basic and translational research of NCCs. Furthermore, these newly-identified genes up-regulated in cNCC would provide helpful information on NC-originating tumors, developmental disorders in NCC derivatives, and potential applications of NCCs in regenerative medicine.