Miracle-dz/UniFAR-FTUs huggingface dataset:
Initial steps in the anaerobic degradation of 3,4,5-trihydroxybenzoate by Eubacterium oxidoreducens: characterization of mutants and role of 1,2,3,5-tetrahydroxybenzene

Chemical mutagenesis and antibiotic enrichment techniques were used to isolate five mutant strains of the obligate anaerobe Eubacterium oxidoreducens that were unable to grow on 3,4,5-trihydroxybenzoate (gallate). Two strains could not transform gallate and showed no detectable gallate decarboxylase activity. Two other strains transformed gallate to pyrogallol and dihydrophloroglucinol but lacked the hydrolase activity responsible for ring cleavage. A fifth strain accumulated pyrogallol, although it contained adequate levels of the enzymes proposed for the complete transformation of gallate to the ring cleavage product. The conversion of pyrogallol to phloroglucinol by cell extract of the wild-type strain was dependent on the addition of 1,2,3,5-tetrahydroxybenzene or dimethyl sulfoxide. This activity was induced by growth on gallate, while the other enzymes involved in the initial reactions of gallate catabolism were constitutively expressed during growth on crotonate. The results confirm the initial steps in the pathway previously proposed for the metabolism of gallate by E. oxidoreducens, except for the conversion of pyrogallol to phloroglucinol.
Gender Policing in the Clinical Setting: Discussion of Sandra Silverman’s “The Colonized Mind: Gender, Trauma, and Mentalization”

This is a discussion of Sandra Silverman’s clinical paper “The Colonized Mind: Gender, Trauma, and Mentalization. ” Her patient, David née Ava, started early in life as a gender variant child. In adolescence, she became more stereotypically gender conforming. She entered treatment with Silverman as a young adult who was feeling great shame about both her past and present gender dysphoria. This discussion focuses on the clinical problem of gender policing, its external and internal manifestations as well as its appearance in the countertransference.
Gender and Entrepreneurship as a Career Choice

The gender gap in entrepreneurship has typically been understood through women’s structural disadvantages in acquiring the resources relevant for successful business ownership. This study builds on resource-based approaches to investigate how cultural beliefs about gender influence the process by which individuals initially come to identify entrepreneurship as a viable labor-market option. Drawing on status characteristics theory, this study evaluates (1) how cultural beliefs about gender and entrepreneurship influence self-assessments of entrepreneurial ability, and (2) the extent to which such assessments account for the gender gap in business start-ups. Results suggest that women are significantly less likely to perceive themselves as able to be an entrepreneur and they hold themselves to a stricter standard of competence when compared to similarly situated men. This gender difference in self-assessments accounts for a significant portion of the gender gap in entrepreneurship after controlling for relevant resources. Additional analyses reveal that significant gender differences in self-assessed ability persist among established business owners.
Fuzzy relevance feedback in content-based 3D model retrieval

Relevance feedback is an iterative search technique to bridge the semantic gap between the high level user intention and low level data representation. This technique interactively determines a user's desired output or query concept by asking the user whether certain proposed 3D models are relevant or not. For a relevance feedback algorithm to be effective, it must grasp a user's query concept accurately and quickly. In this paper, we propose a framework called fuzzy relevance feedback in content-based 3D model retrieval systems. Fuzzy relevance feedback is to integrate the users' fuzzy interpretation of semantic content into the notion of relevance feedback. Experimental results show that this algorithm achieves higher search accuracy than traditional query refinement schemes.
Why do People Disclose their HIV Status? Qualitative Evidence from a Group of Activist Women in Khayelitsha

Abstract This study explores the bio-psychosocial context of HIV/AIDS in which eleven HIV-positive activist women from Khayelitsha negotiated the process of disclosing their HIV-positive status to significant others, specifically biological household members. The study is based on the narratives of a group of HIV-positive peer-educators in Cape Town, South Africa. The results suggest that community perceptions of HIV/AIDS are shaped by a wide variety of factors particular to the socio-cultural and political context of HIV/AIDS in South Africa. The process and outcomes of disclosure to others occurs within this shifting web of meanings, and in turn, reshapes them as people respond to the person who has just disclosed. Eight out of the eleven women disclosed to a close biological household member as these significant-others provided, or had the potential to provide, the appropriate support. Six of the eleven participants disclosed during the symptomatic phases of HIV/AIDS, while five were asymptomatic at the time. Four of the five who disclosed when asymptomatic were diagnosed while pregnant and had never experienced severe opportunistic infections. Additionally, these same four disclosed some time after diagnosis (nine months > t < two years). Of the six who disclosed in a state of ill-health, five disclosed immediately after diagnosis in order to access health-related social support. At this time, concerns regarding health and mortality superseded fears of rejection and discrimination due to AIDS-related stigma. The perceived potential benefits (social and health-related support) outweighed the perceived risks (stigmatisation and discrimination). When participants had never experienced serious illness, they disclosed in order to educate loved-ones, gain emotional support or challenge false popular perceptions of HIV/AIDS.
Race, Gender, and Research

When students enter college classrooms for the first time they inevitably have preconceived images of professors. According to research on student evaluations of teaching, these preconceptions have important implications in college classrooms. This study explores one avenue through which these preconceptions are perpetuated—popular film. Using content analysis, the authors examine popular films released between 1985 and 2005 that contain professors in either primary or secondary roles. The findings show stereotypical depictions beyond glasses, bow ties, and tweed jackets. Specifically, the authors find stereotypical images of race and gender as well as an emphasis on the importance of research, sometimes at the expense of teaching or ethical behavior. This research provides instructors with knowledge of the stereotypes that students may have upon entering the college classroom, which may impact classroom interactions, and provides insight into how race and gender affect student evaluations of professors.
Public affairs as reality construction: an established paradigm with new implications

This article was initially a reaction to the special issues of the Journal of Public Affairs on the relative lack of theoretical foundations for the research and practice of public affairs (Vol. 1 no. 4, Vol. 2 no. 1, 2002). The social-scientific approach that is known under the name constructionism has interesting implications for the study and the practice of this field. The authors explore some of its central concepts and implications such as framing, priming, typification, claims making, symbolic power, background, sense making and narrative construction. They test some of these concepts for their applicability and usefulness for research, theory building and practice in the field of public affairs. Copyright © 2006 John Wiley & Sons, Ltd.
Contrasting patterns of mitochondrial DNA and microsatellite introgressive hybridization between lineages of lake whitefish (Coregonus clupeaformis); relevance for speciation

We performed a combined analysis of mitochondrial DNA (mtDNA) and microsatellite loci among lake whitefish (Coregonus clupeaformis) populations in order to assess the levels of congruence between both types of markers in defining patterns of genetic structuring, introgressive hybridization and inferring population origins in the hybrid zone of the St. John River basin. A second objective was to test the hypothesis that secondary contact between glacial lineages always resulted in the occurrence of sympatric dwarf and normal whitefish ecotypes. Fish were sampled from 35 populations and polymorphism was screened at mtDNA and six microsatellite loci for a total of 688 and 763 whitefish, respectively. Four lakes harbouring a single whitefish population of normal ecotype admixed with mtDNA haplotypes of different lineages were found. This confirmed that secondary contact between whitefish evolutionary lineages did not always result in the persistence of reproductively isolated ecotypes. Microsatellites further supported the definition of distinct glacial lineages by identifying lineage‐specific allelic size groups. They also further supported the hypothesis that ecotypes originated from either a single founding lineage (sympatric divergence) or following secondary contacts between lineages (allopatric divergence), depending on the lake. In general, however, the pattern of population differentiation and introgressive hybridization observed at microsatellites was in sharp contrast with that depicted by mtDNA variation. Both factorial correspondence analysis and analysis of admixture proportion revealed a much more pronounced pattern of introgressive hybridization than depicted by mtDNA analyses. Variable levels of introgression indicated that environmental differences may be as important as the historical contingency of secondary contact in explaining the persistence of sympatric ecotypes and the differential pattern of introgressive hybridization among lakes. Whitefish populations from the St. John River basin hybrid zone represent a rare illustration of a continuum of both morphological and genetic differentiation within a given taxon, spanning from complete introgression to possibly complete reproductive isolation, depending on lakes. Thus, each lake may be viewed as a different temporal snapshot taken throughout the gradual process of speciation.
A Relational Model Based Semantic Network Knowledge Representation Technology and Its Application

According to relational database theory, solid foundation, and mature technology, various products have made it the first consideration for studying and storing massive data. But how to build an effective knowledge base which is based on the powerful storage and processing capability of relational database is not only one of the key issue for the research and application relating to knowledge engineering and intelligent system, but also is the technological bottleneck of how to turn knowledge management into application. In developing the query text data and content management application, there are some issues discovered with using the traditional method to retrieve content based on the contents of the title, author, and time to search. For instance, concerning a large amount of construction work on resources preparing, low query efficiency, and being unable to search out interrelated content and so on. In this paper, a relational model of semantic networks is given and a two-dimensional storage structure of relational data table based on the semantic network knowledge with practical examples is discussed. At the same time, this paper designs the stored procedure based on SQL language description; moreover its key searching skill lies in keywords and the connections among the keywords. The semantic network relational models, storage structure and query algorithm can solve the foregoing problems related to the knowledge or content management. That is to say it is used to inquire the contents of query system of laws and regulations, which gained excellent result. This technique is easy to require without specialized knowledge processing tool. Only a simple training can help people get hold on it.
Frustrated expected reward induces differential transcriptional changes in the mouse brain

Frustration represents a particular aspect of the addictive process that is related to loss of control when the expected reward is not obtained. We aim to study the consequences of frustrated expected reward on gene expression in the mouse brain. For this purpose, we used an operant model of frustration using palatable food as reward combined with microarrays. Transcriptomic profiles of frontal cortex, ventral striatum and hippocampus were analysed in five groups of mice: (1) positive control receiving palatable food and the cue light as conditioned stimulus; (2) frustrated group only receiving the cue light; (3) extinction learning group that did not receive palatable food nor the light; (4) negative control that never received the reinforcer nor the light during the whole experiment; and (5) yoked that received palatable food passively. Gene expression changes produced by frustration were revealed in the frontal cortex and ventral striatum, but not in the hippocampus. Most of the changes, such as the modification of the dopamine‐DARPP‐32 signalling pathway, were common in both areas and estimated to have neuronal origin. Extinction learning induced transcriptional changes only in the ventral striatum, with most genes showing down‐regulation and without alteration in the dopamine‐DARPP‐32 signalling pathway. Active palatable food‐seeking behaviour induced changes in gene expression in ventral striatum mainly affecting cell communication. In conclusion, frustration behaviour‐induced changes in frontal cortex and ventral striatum mainly related to dopamine‐DARPP‐32 signalling that could play an important role in the loss of behavioural control during the addictive processes.
Measurement of Cardiac Troponins

The cardiac troponins form part of the regulatory mechanism for muscle contraction. Specific cardiac isoforms of cardiac troponin T and cardiac troponin I exist and commercially available immunoassay systems have been developed for their measurement. A large number of clinical and analytical studies have been performed and the measurement of cardiac troponins is now considered the ‘gold standard’ biochemical test for diagnosis of myocardial damage. There have been advances in understanding the development and structure of troponins and their degradation following myocardial cell necrosis. This has contributed to the understanding of the problems with current assays. Greater clinical use has also highlighted areas of analytical and clinical confusion. The assays are reviewed based on manufacturers' information, current published material as well as the authors' in-house experience.
Detailed Analyses of Ganzfeld Phenomena as Perceptual Events in Stimulus-Reductive Situations

Experiment 1 reexamined in detail conscious experiences in Ganzfeld situations with homogeneous light (red, green, or blue). Subjects were instructed to report everything, including phenomena attributed to the external world (colour, brightness, space) and those of the self (somatosensory experience, body movement, and emotion). The reports were richer in the red-light condition. Naive subjects reported a larger variety of events than sophisticated subjects. In Experiment 2 pupillary responses were recorded while the subjects were exposed to a Ganzfeld at two different levels of illumination. Pupillary size for high illumination decreased with time and then recovered to the initial level, while for low illumination it remained constant. Subjective brightness correlated with pupillary size. Experiment 3 revealed a temporal pattern of spatial events. The findings obtained are discussed in relation to the ontogenetic process of visual space perception and emotion.
Service-dominant logic: reactions, reflections and refinements

As one of its own foundational premises implies, the value of service-dominant (S-D) logic is necessarily in its open, collaborative effort. Thus, the authors invite and welcome both elaborative and critical viewpoints. Five recurring, contentious issues among collaborating scholars, as they attempt to understand the full nature and scope of S-D logic, are identified. These issues are clarified and refined, as is appropriate to this co-creation of a service-centric philosophy by the worldwide marketing community.
Systems biology and mechanics of growth

In contrast to inert systems, living biological systems have the advantage to adapt to their environment through growth and evolution. This transfiguration is evident during embryonic development, when the predisposed need to grow allows form to follow function. Alterations in the equilibrium state of biological systems breed disease and mutation in response to environmental triggers. The need to characterize the growth of biological systems to better understand these phenomena has motivated the continuum theory of growth and stimulated the development of computational tools in systems biology. Biological growth in development and disease is increasingly studied using the framework of morphoelasticity. Here, we demonstrate the potential for morphoelastic simulations through examples of volume, area, and length growth, inspired by tumor expansion, chronic bronchitis, brain development, intestine formation, plant shape, and myopia. We review the systems biology of living systems in light of biochemical and optical stimuli and classify different types of growth to facilitate the design of growth models for various biological systems within this generic framework. Exploring the systems biology of growth introduces a new venue to control and manipulate embryonic development, disease progression, and clinical intervention. WIREs Syst Biol Med 2015, 7:401–412. doi: 10.1002/wsbm.1312
Ultra high-speed data signals with alternating and pairwise alternating optical phases

We demonstrate two 160-Gb/s modulation formats that posses the same intensity pulse profile but differ regarding their phase coding. We analyze the nonlinear transmission properties of both coherent modulation formats by experimental and analytical investigations. The results show that the pairwise alternating phase coded carrier suppressed return-to-zero (PAP-CSRZ) signal outperforms the conventional CSRZ format. Our modulation technique is even applicable at higher speed as demonstrated with the generation of the first CSRZ signal at a record rate of 320 Gb/s.
Beyond Self-Concept and Self-Esteem for African American Students: Improving Racial Identity Improves Achievement

While it is recognized that self-concept and self-esteem affect the academic achievement of students, few publications have focused on the affective and psychological needs of students who are gifted and ethnically or culturally diverse. In this article, we extend the discussion of self-concept and achievement by focusing on how racial identity development affects the achievement of gifted African American students. We argue that few efforts, designed to improve gifted Black students' achievement and social-emotional well-being, will be successful until educators focus specifically on their racial identity. While we acknowledge that a focus on racial identity is necessary for all African American students, we are most concerned in this article with gifted Black students because so few have been identified as gifted. Along with describing racial identity development and issues facing gifted African American students, we offer solutions for change.
Amongst Women: Exploring the Reality of Rural Childcare

In this paper we examine the provision and use of rural childcare, drawing on original data collected as part of a study of formal and informal childcare services in the County of Devon in England. The paper goes beyond existing discussions of childcare in rural areas in attempting to place patterns of use in the broader context of household gender relations and the cultural construction of rurality. We argue that decisions around child care by rural families need to be considered in relation not only to the availability of different sorts of service but also to the employment aspirations and choices of men and women and the assumptions of the primacy of women’s mothering role. Such issues are underpinned, we contest, by idyllic constructions of rural family life and social relations.
The Major Cell Populations of the Mouse Retina

We report a quantitative analysis of the major populations of cells present in the retina of the C57 mouse. Rod and cone photoreceptors were counted using differential interference contrast microscopy in retinal whole mounts. Horizontal, bipolar, amacrine, and Müller cells were identified in serial section electron micrographs assembled into serial montages. Ganglion cells and displaced amacrine cells were counted by subtracting the number of axons in the optic nerve, learned from electron microscopy, from the total neurons of the ganglion cell layer. The results provide a base of reference for future work on genetically altered animals and put into perspective certain recent studies. Comparable data are now available for the retinas of the rabbit and the monkey. With the exception of the monkey fovea, the inner nuclear layers of the three species contain populations of cells that are, overall, quite similar. This contradicts the previous belief that the retinas of lower mammals are “amacrine-dominated”, and therefore more complex, than those of higher mammals.
S2I+: Armazenamento Eficiente em Índice Espaco-Textual

Com a popularização dos Smartphones com GPS, há uma grande quantidade de dados com informações espaciais e textuais sendo produzidos e compartilhados. Para realizar consultas de forma eficiente sobre esses dados, faz-se necessário utilizar índices híbridos que combinam índices espaciais e textuais. Um dos índices com melhor desempenho é o S2I (Spatial Inverted Index). Infelizmente, o S2I ocupa muito espaço para armazenamento. Este trabalho propõe novas estratégias para reduzir o tamanho do S2I, mantendo os bons aspectos em termos de desempenho.
Lethal and mutagenic interactions between γ-rays, cisplatin and etoposide at the cellular and molecular levels

Purpose: We analysed the lethal and mutagenic interactions between γ-rays, cisplatin (Pt) and etoposide (E), three agents used in tumour chemoradiotherapy. Corresponding results at cellular and molecular levels could provide additional elements on involved mechanisms and, on antitumour activity and toxicity in combined cancer treatments. Materials and methods: The yeast Saccharomyces cerevisiae SC7K(lys2–3) (auxotrophic for lysine) was used as eukaryotic model. Exponential growing cells were exposed to the mentioned agents, as single and combined treatments. Lethal and mutation interaction equations were determined as a function of doses according to quantitative models. DNA double-strand breaks were evaluated immediately after treatments, through pulsed-field electrophoresis and laser densitometry. Results: All three agents induced significant mutant frequency. The γ +Pt + E combination determined maximal lethal and mutagenic synergism, followed by γ + Pt and γ + E combinations. Meanwhile, Pt + E combination showed lethal additivity and very low mutagenic synergism. Pt + E double combination determined moderate DNA degradation. DNA degradation after γ-exposure, was similar to that of γ + Pt, γ + E and γ + Pt + E combinations. Conclusions: Synergistic lethal and mutagenic interactions indicate crosstalk between non-homologous end joining, homologous recombination and postreplicative repair pathways. Pt + E additivity indicate independence of involved repair pathways. Furthermore, the quantification of interactive events may be an additional suitable tool in tumour therapy planning.