Miracle-dz/UniFAR-FTUs huggingface dataset:
Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space

The capacity to respond to temperature fluctuations is critical for microorganisms to survive within mammalian hosts, and temperature modulates virulence traits of diverse pathogens. One key temperature-dependent virulence trait of the fungal pathogen Candida albicans is its ability to transition from yeast to filamentous growth, which is induced by environmental cues at host physiological temperature. A key regulator of temperature-dependent morphogenesis is the molecular chaperone Hsp90, which has complex functional relationships with the transcription factor Hsf1. Although Hsf1 controls global transcriptional remodeling in response to heat shock, its impact on morphogenesis remains unknown. Here, we establish an intriguing paradigm whereby overexpression or depletion of C. albicans HSF1 induces morphogenesis in the absence of external cues. HSF1 depletion compromises Hsp90 function, thereby driving filamentation. HSF1 overexpression does not impact Hsp90 function, but rather induces a dose-dependent expansion of Hsf1 direct targets that drives overexpression of positive regulators of filamentation, including Brg1 and Ume6, thereby bypassing the requirement for elevated temperature during morphogenesis. This work provides new insight into Hsf1-mediated environmentally contingent transcriptional control, implicates Hsf1 in regulation of a key virulence trait, and highlights fascinating biology whereby either overexpression or depletion of a single cellular regulator induces a profound developmental transition.
An Investigation into the Challenges and Opportunities Associated with the Application of Internet of Things (IoT) in the Agricultural Sector-A Review

Internet of things has acquired attention all over the globe. It has transformed the agricultural field and allowed farmers to compete with massive issues they face. The aim of this paper is to review the various challenges and opportunities associated with the applications of internet of things in agricultural sector. This research makes use of secondary sources that have been gathered from existing academic literature such as journals, books, articles, magazines, internet, newsletter, company publications and whitepapers. Applications reviewed in this research are about crop sensing, mapping and monitoring the croplands pattern, managing and controlling with the help of radio frequency identification and real-time monitoring of environment. Some of the challenges that were taken into consideration for reviewing the applications of internet of things are software complexity, security, lack of supporting infrastructure and technical skill requirement. Complexity in the software has to be rectified in order to support the IoT network. Therefore software must be developed as user-friendly for improving the farming, production and quality of the crop. Security is the major threat in the IoT applications. Security has to be enhanced through proper access control, data confidentiality and user authentication. Technical skill is required for farming to enhance the organizational abilities and to perform the farming functions, solving problems and more. Proper supporting infrastructure can be developed with proper internet availability and connectivity. Some of the opportunities were taken for reviewing the applications of internet of things are low power wireless sensor, better connectivity, operational efficiency and remote management.
Biodegradation of 1,2,3-Trichloropropane through Directed Evolution and Heterologous Expression of a Haloalkane Dehalogenase Gene

ABSTRACT Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering of a chloropropanol-utilizing bacterium, we constructed an organism that is capable of growth on 1,2,3-trichloropropane (TCP). This highly toxic and recalcitrant compound is a waste product generated from the manufacture of the industrial chemical epichlorohydrin. Attempts to select and enrich bacterial cultures that can degrade TCP from environmental samples have repeatedly been unsuccessful, prohibiting the development of a biological process for groundwater treatment. The critical step in the aerobic degradation of TCP is the initial dehalogenation to 2,3-dichloro-1-propanol. We used random mutagenesis and screening on eosin-methylene blue agar plates to improve the activity on TCP of the haloalkane dehalogenase from Rhodococcus sp. m15-3 (DhaA). A second-generation mutant containing two amino acid substitutions, Cys176Tyr and Tyr273Phe, was nearly eight times more efficient in dehalogenating TCP than wild-type dehalogenase. Molecular modeling of the mutant dehalogenase indicated that the Cys176Tyr mutation has a global effect on the active-site structure, allowing a more productive binding of TCP within the active site, which was further fine tuned by Tyr273Phe. The evolved haloalkane dehalogenase was expressed under control of a constitutive promoter in the 2,3-dichloro-1-propanol-utilizing bacterium Agrobacterium radiobacter AD1, and the resulting strain was able to utilize TCP as the sole carbon and energy source. These results demonstrated that directed evolution of a key catabolic enzyme and its subsequent recruitment by a suitable host organism can be used for the construction of bacteria for the degradation of a toxic and environmentally recalcitrant chemical.
Design and characterization of PDO biodegradable intravascular stents

Two novel biodegradable intravascular stents (BIS) with different structures are introduced. Braiding-structural BIS and Z-structural BIS were fabricated from polydioxanone (PDO) monofilament by a hand-braiding method with a perforated mold, imitating commercial stents that have been used clinically. The fabrication process of these two BIS is described and stent parameters, mechanical properties, and degradation properties are reported. The findings reveal that Z-structural BIS have higher porosity, smaller longitudinal shortening rate, and higher radial force and recovery rate compared with the braiding-structural stent. During the degradation process, braiding-structural BIS maintained their mechanical properties higher than international standards for 12 weeks, while Z-structural stents maintained them for 16 weeks.
The Impact of Competition on "Make-or-Buy" Decisions: Evidence from the Spanish Local TV Industry

This paper empirically investigates whether changes in product market competition affect firm boundaries. Exploiting regulation-induced shocks to entry barriers and differences in regulation enforcement across cities to obtain exogenous variation in competition, we establish a negative causal effect of competition (through reduced entry barriers and a larger number of rival firms) on vertical integration in the setting of the Spanish local television industry between 1995 and 2002. Data and the online appendix are available at https://doi.org/10.1287/mnsc.2016. 2633. This paper was accepted by Bruno Cassiman, business strategy.
Environmental effects in fibre fabrication using electrospinning-based rapid prototyping

ABSTRACT The ability to construct rapidly artefacts with controllable architectures has made rapid prototyping (RP) an attractive alternative for scaffold fabrication in tissue engineering. Recently, an electrospinning-based RP, another in-house development RP technique, has been introduced. The technique applies near-field electrospinning for fine fibre creation and faces a problem on fibre solidification influenced by several parameters. Presented in this paper is the investigation on the effect of ambient parameters on the fibre fabrication process. Experiments were conducted to investigate the fabrication in open and closed environments as well as the influence of humidity. According to the statistical analyses of the results, the environment had slight effects on the fibre creation, and the fabrication in the closed environment is recommended.
Cell-cell signaling interactions coordinate multiple cell behaviors that drive morphogenesis of the lateral line

The zebrafish sensory lateral line system has emerged as a powerful model for the mechanistic study of collective cell migration and morphogenesis. Recent work has uncovered the details of a signaling network involving the Wnt/β-catenin, Fgf and Delta-Notch pathways that patterns the migrating lateral line primordium into distinct regions. Cells within these regions exhibit different fundamental behaviors that together orchestrate normal lateral line morphogenesis. In this review, we summarize the signaling network that patterns the migrating lateral line primordium and describe how this patterning coordinates crucial morphogenic cell behaviors.
Interactions With Robots: The Truths We Reveal About Ourselves

&NA; In movies, robots are often extremely humanlike. Although these robots are not yet reality, robots are currently being used in healthcare, education, and business. Robots provide benefits such as relieving loneliness and enabling communication. Engineers are trying to build robots that look and behave like humans and thus need comprehensive knowledge not only of technology but also of human cognition, emotion, and behavior. This need is driving engineers to study human behavior toward other humans and toward robots, leading to greater understanding of how humans think, feel, and behave in these contexts, including our tendencies for mindless social behaviors, anthropomorphism, uncanny feelings toward robots, and the formation of emotional attachments. However, in considering the increased use of robots, many people have concerns about deception, privacy, job loss, safety, and the loss of human relationships. Human–robot interaction is a fascinating field and one in which psychologists have much to contribute, both to the development of robots and to the study of human behavior.
Alcohol and cardiovascular disease in the geriatric population

There has been little focus on the effects of alcohol on the elderly. Although the cardiovascular benefits of moderate alcohol consumption could be of the greatest benefit in this group, so might be the detrimental effects of abuse. In this article, we review available data on the effects of alcohol consumption on cardiovascular disease, cardiomyopathy, arrhythmias, hypertension, and vascular function in older adults. Alcohol consumption has increased in the US population age 65 years and older in the last decade, as has monthly heavy episodic drinking in older alcohol consumers. Studies of alcohol consumption in older subjects suggest that consumption in moderation does not increase the risk of heart failure, hypertension, or atrial arrhythmias, and may in fact improve vascular function and reduce cardiovascular disease events. As in younger subjects, heavy consumption, or abuse of alcohol, negates any potential protective cardiovascular effects, increasing the incidence of heart failure and hypertension. Although alcohol consumed in moderation does not appear harmful in the elderly population, heavier consumption exacerbates hypertension and increases the incidence of heart failure.
TRPC3 overexpression and intervention in airway smooth muscle of ovalbumin‐induced hyperresponsiveness and remodeling

Transient receptor potential canonical channel 3 (TRPC3) proteins function as non‐voltage‐gated Ca2+‐permeable channels and play divergent roles in many processes of pathophysiology. The purpose of this study was to determine the relationship between TRPC3 expression and airway hyperresponsiveness and remodeling in ovalbumin‐induced asthmatic Kunming mice. Mice were sensitized and challenged by ovalbumin to establish asthmatic model. Hematoxylin‐eosin staining, hydroxyproline assay, and isometric tracheal ring force measurement were used to evaluate airway remodeling and hyperresponsiveness in asthmatic mice. Western blot was performed to detect the expression of TRPC3 proteins. MTT assay was used to measure the proliferation of airway smooth muscle cells. TRPC3 protein expression increased in airway smooth muscle of asthmatic mice. GdCl3, a nonspecific TRPC blocker, attenuated the contractile force of airway smooth muscle. Fetal bovine serum stimulated airway smooth muscle cells proliferation and augmented TRPC3 protein expression. Both TRPC3 blockade by GdCl3 or specific TRPC3 antibodies and gene silencing by siRNA inhibited the proliferation of airway smooth muscle cells. In contrast, the current drugs treatment for asthma such as Dexamethasone and Aminophylline had no effects on TRPC3 protein overexpression. Therefore, TRPC3 protein overexpression may be involved in airway smooth muscle hyperresponsiveness and remodeling in asthmatic mice, providing evidence for a new direction of asthma pathogenesis research and a new target for drug intervention.
CHOOSING WISELY ® : NEXT STEPS IN HEALTHCARE VALUE Hospitalist Value in an ACO World

The accountable care organization (ACO) concept, elucidated in 2006 as the development of partnerships between hospitals and physicians to coordinate and deliver efficient care,1 seeks to remove existing barriers to improving value.2 Some advocate this concept as a promising payment model that could successfully realign the current payment system to financially reward improvements in quality and efficiency that bend the cost curve.3,4 Hospitalists fit well with this philosophy. As the fastest growing medical specialty in the history of American medicine, from a couple of thousand hospitalists in the mid-1990s to more than 50,000, the remarkable progression of hospitalists has ostensibly been driven partially by hospitals’ efforts to improve the value equation through enhanced efficiency in inpatient care. Importantly, hospitalists probably provide care for more than half of all hospitalized Medicare beneficiaries and increasingly patients in skilled nursing facilities (ie, SNFists). 5 Along with primary care physicians, hospitalists thus represent an essential group of physicians needed to transform care delivery.
Understanding the Evolution of Code Smells by Observing Code Smell Clusters

Code smells are more likely to stay inter-connected in software rather than remaining as a single instance. These code smell clusters create maintainability issues in evolving software. This paper aims to understand the evolution of the code smells in software, by analyzing the behavior of these clusters such as size, number and connectivity. For this, the clusters are first identified and then these characteristics are observed. The identification of code smell clusters is performed in three steps - detection of code smells (God Class, Long Method, Feature Envy, Type Checking) using smell detection tools, extraction of their relationships by analyzing the source code architecture, and generation of graphs from the identified smells and their relationships, that finally reveals the smelly clusters. This analysis was executed on JUnit as a case study, and four important cluster behaviors were reported.
Prediction of bleeding events in patients with venous thromboembolism on stable anticoagulation treatment

Attempts at identifying patients with an elevated risk of bleeding while on anticoagulation following acute venous thromboembolism (VTE) have largely been unsuccessful thus far. We sought to develop a clinical prediction score for bleeding during stable anticoagulation treatment after acute VTE. We performed a post hoc analysis of the pooled RE-COVER studies, two double-blind randomised “sister” trials evaluating dabigatran versus standard treatment in 5107 VTE patients. A score was derived from patients randomised to dabigatran using logistic regression analysis covering the complete follow-up period. The final model, named VTE-BLEED, included six variables and yielded a c-statistic of 0.72 (95% CI 0.67–0.76). Patients from the derivation cohort in the low-risk group (<2 points; 74% of the derivation population) had a bleeding incidence of 2.8% compared to 12.6% in the elevated-risk group (OR 5.0; 95% CI 3.5–7.1). The score proved accurate for our primary end-point, i.e. prediction of major bleeding after day 30 (“stable” anticoagulation), both in patients on dabigatran (c-statistic 0.75, 95% CI 0.61–0.89) and those on warfarin (0.78, 95% CI 0.68–0.86; p=0.77 for difference). The new VTE-BLEED score accurately predicted major bleeding events in VTE patients on stable anticoagulation with both dabigatran and warfarin. The new VTE-BLEED score predicted major bleeding events in VTE patients on stable anticoagulation treatment http://ow.ly/TxED300SpwI
Current progress in DNA barcoding and future implications for entomology

DNA barcoding is a technique for identifying organisms based on a short, standardized fragment of genomic DNA. The standardized sequence region is called a DNA barcode because it is like a barcode tag for each taxon. Since the proposition of this concept and the launch of a large project named the Barcode of Life, this simple technique has attracted attention from taxonomists, ecologists, conservation biologists, agriculturists, plant‐quarantine officers and others, and the number of studies using the DNA barcode has rapidly increased. The extreme diversity of insects and their economical, epidemiological and agricultural importance have made this group a major target of DNA barcoding. However, there is some controversy about the utility of DNA barcoding. In this review, we present an overview of DNA barcoding and its application to entomology. We also introduce current advances and future implications of this promising technique.
A Pattern Language Based Learning Design Studio for an Analytics Informed Inter-Professional Design Community

Over the past ten years, a lot of advances have been made in both the learning design and learning analytics research communities in terms of research outputs. On the other hand, these advances have not made visible impact on the everyday practices of the key stakeholders they aim to serve: teachers and learners. This paper argues that to advance both fields and to connect them into an iterative cycle of continuous improvement, we need a learning design pattern language in the Alexandrian tradition to guide learning design practice, and to connect designers’ intuitive formulation of questions about learning processes and outcomes with evidence-based questions and operationalizable questions. It further describes the pattern language and the online tool, Learning Design Studio that the authors have developed to serve as an organically extensible language and a technology platform to support learning design, and inter-professional collaboration among learning designer and learning analytics communities.
Investigating Genres and Perspectives in HCI Research on the Home

The home and domestic experiences have been studied from multiple points of view and disciplines, with an array of methodologies in the past twenty-five years in HCI. Given the attention to the home and the volume of research, what further areas of research might there be? Based on a critical analysis of 121 works on the topic, we present seven genres of domestic technology research in HCI: social routines in the home, ongoing domestic practices, the home as a testing ground, smart homes, contested values of a home, the home as a site for interpretation, and speculative visions of the home. We articulate dominant research perspectives in HCI, and we offer two complementary perspectives about how to investigate the domestic experience in future research: the material perspective and the first person perspective.
Acoustic Emission Analysis of Cement Mortar Specimens During Three Point Bending Tests

THIS WORK DISCUSSES THE EXPERIMENTAL RESULTS OF ACOUSTIC EMISSION (AE) RECORDINGS DURING REPETITIVE LOADING/UNLOADING LOOPS PRO-CESSES OF CEMENT MORTAR BEAMS SUBJECTED TO THREE-POINT BENDING. SIX REPETITIVE LOADING CYCLES WERE CONDUCTED AT A GRADUALLY HIGHER LOAD LEVEL UNTIL THE FAILURE OF THE SPECIMENS. THE EXPERIMENTAL RESULTS CLEARLY SHOW THE EXISTENCE AND DOMINANCE OF THE KAISER EFFECT DURING EACH LOADING LOOP. REGARDING THE AE DATA, ALTERNA-TIVE ANALYSIS WAS CONDUCTED USING THE IMPROVED B-VALUE, AND THE CUMULATIVE ENERGY BEHAVIOUR. BOTH QUANTITIES CONSIDERED, SHOW QUALITATIVE AND QUANTITATIVE CHARACTERISTICS THAT COULD BE USED AS PRE-FAILURE INDICATORS. IN ADDITION, A NOVEL STATISTICAL PHYSICS ANAL-YSIS INVOLVING THE AE INTEREVENT TIMES WAS CONDUCTED BY CALCULAT-ING THE CUMULATIVE PROBABILITY FUNCTION P(>I´I) THAT FOLLOWS A Q-EXPONENTIAL EQUATION. THE ENTROPIC INDEX Q AND THE RELAXATION PARAMETER I²Q OF THIS EQUATION SHOW SYSTEMATIC CHANGES DURING THE DIFFERENT STAGES OF THE FAILURE PROCESS. THE LAST CYCLE LED TO A Q VALUE EQUAL TO 1.42, IMPLYING THE UPCOMING FRACTURE WHICH IS IN GOOD AGREEMENT WITH PREVIOUS RESULTS OBTAINED FROM A WIDE RANGE OF FRACTURED MATERIALS.
Characterization of Fungal Biofilms within a Municipal Water Distribution System

ABSTRACT Biofilms of a municipal water distribution system were characterized to assess the occurrence of fungi within surface matrixes. Densities of filamentous fungi ranged from 4.0 to 25.2 CFU cm−2, whereas yeast densities ranged from 0 to 8.9 CFU cm−2. Observations by scanning electron microscopy further suggested that spores, not hyphae or vegetative cells, comprised the primary source of viable propagules.
Direct semi-blind design of serial linear equalizers for doubly-selective channels

Recently, serial linear equalizers (SLEs) and serial decision feedback equalizers (SDFEs) have been proposed to mitigate the doubly-selective channel effects. To design the SLE/SDFE and to model the doubly-selective channel, a so-called finite impulse response basis expansion model (FIR-BEM) is used. Initially, the FIR-BEM coefficients of the SLE/SDFE were designed based on the exact knowledge of the FIR-BEM coefficients of the doubly-selective channel. In practice, we can use a direct SLE/SDFE design procedure, which avoids an intermediate channel estimation step. In this paper, we describe this idea for the SLE and focus on direct semi-blind design of the FIR- BEM coefficients of the SLE. Simulation results demonstrate the validity of the proposed approach.
Comparative analysis of MCU memory for IoT application

Low power consumption is very important for Internet of Things (IoT) for achieving long lifetime. On the other hand, non-volatile memory such as Flash memory or Ferroelectronic Random Access Memory (FRAM) has been commercially produced for power reduction in micro control unit (MCU). In this paper, the MCU based on the Flash and FRAM are compared and analyzed in terms of power through IoT implementation. For practical analysis, heating, ventilation, and air conditioning (HVAC) is implemented and measured based on MCU. From measurement results, the time and power consumption of shutdown and wakeup are important as that of the active and standby.
Role of cyclooxygenase in the vascular responses to extremity cooling in Caucasian and African males

What is the central question of this study? Compared with Caucasians, African individuals are more susceptible to non‐freezing cold injury and experience greater cutaneous vasoconstriction and cooler finger skin temperatures upon hand cooling. We investigated whether the enzyme cyclooxygenase is, in part, responsible for the exaggerated response to local cooling. What is the main finding and its importance? During local hand cooling, individuals of African descent experienced significantly lower finger skin blood flow and skin temperature compared with Caucasians irrespective of cyclooxygenase inhibition. These data suggest that in young African males the cyclooxygenase pathway appears not to be the primary reason for the increased susceptibility to non‐freezing cold injury.