Miracle-dz/UniFAR-FTUs huggingface dataset:
Microanatomical and Biochemical Origins of Normal and Precancerous Cervical Autofluorescence Using Laser-scanning Fluorescence Confocal Microscopy¶

Abstract Clinical studies have shown that in vivo fluorescence spectroscopy can improve the diagnosis of cervical precancer. Recent work suggests that epithelial fluorescence increases, whereas stromal fluorescence decreases, with precancer. However, the microanatomic and biochemical sources of fluorescence in living cervical tissue have not yet been established. This study aims to characterize the origins of living normal and precancerous cervical fluorescence at microscopic levels using laser-scanning fluorescence confocal microscopy. Ten pairs of colposcopically normal and abnormal biopsies were obtained; transverse, 200 μm thick, short-term tissue cultures were prepared and imaged when viable with UV (351–364 nm) and 488 nm excitation before and after addition of the vital dye, Mitotracker Orange. In normal epithelium basal epithelial cells showed cytoplasmic fluorescence; parabasal, intermediate and superficial cells showed fluorescence only at the periphery of the cell. In low-grade precancers cytoplasmic fluorescence was visible in the bottom one-third of the epithelium; in high-grade precancers cytoplasmic fluorescence was visible throughout the lower two-thirds of the epithelium. Cytoplasmic fluorescence was colocalized with the MitoTracker probe and is attributed to mitochondrial reduced form of nicotinamide adenine dinucleotide at UV excitation and mitochondrial flavin adenine dinucleotide at 488 nm excitation. Stromal fluorescence originated from matrix fibers; with the development of precancer the density and fluorescence intensity of matrix fibers decrease. Autofluorescence properties of precancerous cervix reflect an increased number of metabolically active mitochondria in epithelial cells and a reduced stromal fluorescence, which can be an indicator for altered communication between precancerous epithelium and stroma. These changes can explain differences in in vivo fluorescence spectra of normal and precancerous cervical tissue.
Deep-Sequencing Analysis of the Mouse Transcriptome Response to Infection with Brucella melitensis Strains of Differing Virulence

Brucella melitensis is an important zoonotic pathogen that causes brucellosis, a disease that affects sheep, cattle and occasionally humans. B. melitensis strain M5-90, a live attenuated vaccine cultured from B. melitensis strain M28, has been used as an effective tool in the control of brucellosis in goats and sheep in China. However, the molecular changes leading to attenuated virulence and pathogenicity in B. melitensis remain poorly understood. In this study we employed the Illumina Genome Analyzer platform to perform genome-wide digital gene expression (DGE) analysis of mouse peritoneal macrophage responses to B. melitensis infection. Many parallel changes in gene expression profiles were observed in M28- and M5-90-infected macrophages, suggesting that they employ similar survival strategies, notably the induction of anti-inflammatory and antiapoptotic factors. Moreover, 1019 differentially expressed macrophage transcripts were identified 4 h after infection with the different B. melitensis strains, and these differential transcripts notably identified genes involved in the lysosome and mitogen-activated protein kinase (MAPK) pathways. Further analysis employed gene ontology (GO) analysis: high-enrichment GOs identified endocytosis, inflammatory, apoptosis, and transport pathways. Path-Net and Signal-Net analysis highlighted the MAPK pathway as the key regulatory pathway. Moreover, the key differentially expressed genes of the significant pathways were apoptosis-related. These findings demonstrate previously unrecognized changes in gene transcription that are associated with B. melitensis infection of macrophages, and the central signaling pathways identified here merit further investigation. Our data provide new insights into the molecular attenuation mechanism of strain M5-90 and will facilitate the generation of new attenuated vaccine strains with enhanced efficacy.
Semantic Role Labeling Using Support Vector Machines

A static-dissipating laminate is made, containing at least a bottom core layer and a top decorative layer, both layers being impregnated with a resin, where at least the decorative layer has contacting, long carbon fibers uniformly distributed through it in an amount effective to provide an antistatic effect to the laminate, so that static charges accumulating on the top of the decorative layer are dissipated.
Parental support exceeds parenting style for promoting active play in preschool children

Emerging evidence suggests that parenting style may directly or indirectly influence school-aged children's activity behaviour. Given that relatively fewer studies have been conducted among preschool-aged children, this study's primary purpose was to examine the direct relationships between parental support and parenting style on preschool children's active play behaviour, and determine whether parenting style acts as an effect modifier. One hundred and ninety-five parents completed a questionnaire assessing their parental support, parenting style, and their child's active play behaviour. While controlling for parenting style, parental support was positively associated with active play behaviour (β = 0.30, p < 0.001). However, parenting style was not directly associated with active play nor did it moderate the relationship between parental support and child active play (β = 0.04, p = 0.90). Thus, to establish healthy active play behaviours in early child development, parents are encouraged to provide sufficient levels of active play-related support.
Clinical and subclinical intestinal inflammation assessed by the mucosal patch technique: studies of mucosal neutrophil and eosinophil activation in inflammatory bowel diseases and irritable bowel syndrome

Background and aims: There is a clear need for a rapid, simple, safe, and sensitive method of determining the type and intensity of inflammation in the gut mucosa in clinical practice. In this study, we have evaluated the potential of a new method, the mucosal patch technique, in patients with and without apparent gut inflammation, as assessed by conventional diagnostic procedures. Subjects and methods: The technique tested is based on the idea that inflammatory mediators released from the rectal mucosa can be absorbed by and then extracted from cellulose patches brought into contact with the mucosa by use of an instrument with an inflatable balloon. Measurements were performed in healthy controls (n = 16) and in patients with active (n = 19) and inactive ulcerative colitis (UC, n = 8), collagen colitis (CC, n = 12), coeliac disease (n = 13), and irritable bowel syndrome (IBS, n = 13). Results: Inflammatory mediators from neutrophils (myeloperoxidase (MPO)) and eosinophils (eosinophil cationic protein (ECP)) were increased on average 300- and 10-fold, respectively, in patients with active UC compared with healthy controls and were correlated with the endoscopic score. Patients with inactive UC, CC, coeliac disease, and IBS exhibited no endoscopic signs of inflammation. These patient groups had significantly lower levels of MPO and ECP than the active UC group but showed on average a four- to sevenfold increase in MPO compared with healthy controls. Conclusion: The mucosal patch technique was well tolerated by patients and easily applied by the investigator. Pronounced neutrophil and eosinophil involvement in UC was demonstrated. With the high sensitivity of the technique, low degree mucosal neutrophil activation could also be quantified in patients with CC and UC in clinical remission. The finding of increased neutrophil involvement in patients with IBS contributes to the pathophysiological ideas of this disease.
A simplified state-space model of biventricular assist device-cardiovascular system interaction

A simplified state-space model of biventricular assist device (BiVAD)-cardiovascular system (CVS) interaction is presented. The state-space equations includes a six-compartments CVS model incorporating the ventricles, the pulmonary and systemic circulations as well as the non-linear behavior of the valve flow, together with a left ventricular assist device (LVAD) and a right ventricular assist device (RVAD) component. The left and right pump speeds serve as the input variables for the state-space model. The model is simulated with three operational modes, i.e. (i) RVAD speed <; LVAD speed, (ii) same LVAD and RVAD speeds and (iii) inclusion of a 6 mm restriction at the right outflow cannula. The effect of RVAD speed variation on the steady state hemodynamics is also studied with and without an outflow banding restriction. Our simulated results are validated with experimental data obtained from clinical, in vivo and in vitro studies provided in the literatures. We observed that despite its simplicity, the model is able to reproduce the observed trends in the reported studies, thus making it feasible for the development of robust yet practical control algorithms.
A Proposal for a Generic Real-Time Ethernet System

After the introduction of fieldbus technology, real-time Ethernet (RTE) systems now form the second generation of industrial communication systems (2G ICS). A lot of different approaches for RTEs appeared during the last years, most of them optimized for specific use cases or application domains. From a user's perspective, the transition from a proved fieldbus system to a RTE only makes sense, if multiple system properties are improved significantly in comparison to the state-of-the-art. In order to address such main requirements of machine builders, we introduced three building blocks as key components for a generic RTE system, these includes topology-based addressing, optimized datagram transfer, and synchronous scheduling.
Changes in the intraocular pressure of fellow untreated eyes following uncomplicated trabeculectomy.

BACKGROUND AND OBJECTIVE Previous studies have reported changes in the intraocular pressure (IOP) of fellow eyes following unilateral trabeculectomy or selective laser trabeculoplasty. This study evaluates changes in the IOP of fellow eyes following unilateral trabeculectomy and examines correlations of findings with clinical information. PATIENTS AND METHODS Sixty-one patients who underwent unilateral uncomplicated trabeculectomy were included. The IOP of both eyes was recorded on postoperative days 2, 3, 4, 15, 30, 60, and 90. Differences between preoperative and postoperative IOP in both eyes and correlations with clinical parameters were examined. RESULTS The IOP of the fellow eyes was significantly reduced on the fourth postoperative day. This reduction was significantly correlated (inversely) with central corneal thickness of fellow eyes and with the level of IOP reduction to the operated eyes. CONCLUSION The IOP of the fellow eyes remains largely unchanged during the first 3 months following trabeculectomy. However, a significant short-term reduction is noted on the fourth day, implying that unknown (possibly neurocrine) mechanisms may participate in the regulation of the IOP of fellow eyes.
Outcome Analysis of Intra-Articular Scapula Fracture Fixation with Distal Radius Plate: A Multicenter Prospective Study

Background Scapula fractures occur in approximately 1% of all fractures and constitute about 3% - 5% of all injuries of the shoulder joint. Objectives This study aimed to evaluate the clinical outcomes of 20 surgically treated patients with displaced glenoid fractures after stabilization with distal radius plate. Methods Between 2012 and 2015, at 2 centers (HMCH & SHCE) of Bhubaneswar Odisha, we stabilized 20 scapular intra-articular fractures surgically with distal radius locking plate and studied the outcome of the surgeries. The outcome of the 20 fractures was determined using the Constant and Murley score. Both shoulders were assessed and the score on the injured side was given as a percentage of that on the uninjured side. Results The median score was 88% (mean 65%, range 30 to 100). The median score for strength was 21/25 (mean 19, range 0 to 25) and that for pain 11/15 (mean 11, range 5 to 15). The median functional score was 16/20 (mean 15, range 0 to 20). The mean range of active abduction of the shoulder was 135° (20 to 180), the mean range of flexion 138° (20 to 180) and the mean range of external rotation 38° (0 to 100). Five patients showed excellent result; 11 patients showed good result; three patients showed fair result and one patient had poor outcome according to the Constant-Murley score. A superficial infection settled with antibiotics after operation in one patient whose score at final follow-up was 96%. In one patient, delayed healing was reported because of infection. One patient with stiffness of the shoulder at six weeks underwent manipulation under anesthesia with a follow-up score of 81%. Conclusions Various fixation modalities have been described in the literature, however fixation of intra-articular fracture of glenoid with distal radius locking plate for articular reconstruction in the presented series provides good functional outcome with early restoration of the range of motion of the shoulder.
Gender Social and Personal Identity, Sex Dissimilarity, Relationship Conflict, and Asymmetrical Effects

Research on the linkage between sex diversity and relationship conflict has yielded inconsistent findings. In efforts to address this inconsistency and to better understand what contributes to group member perceptions of relationship conflict, interrelationships among sex dissimilarity, gender identity, and relationship conflict were examined utilizing theoretical frameworks from the literatures on identity, status, sex diversity, and asymmetrical effects. Results show that gender social identity moderated the effects of sex dissimilarity on relationship conflict such that in the presence of a strong gender social identity, sex dissimilarity increased perceptions of relationship conflict. This effect was stronger for men than for women, such that men with strong gender social identities in groups in which they were sex dissimilar had greater perceptions of relationship conflict. In addition, a significant three-way interaction was found, in which sex-dissimilar individuals with strong gender social and personal identities perceived a particularly high level of relationship conflict.
A simple method of controlling real and reactive power for a grid-tied inverter

The integration of renewable energy sources into an existing grid requires grid-tied inverters. The reliable and accurate dispatch of real and reactive power to the grid is achieved by controlling the inverters. Recent trend is to operate such grid-tied inverters as virtual synchronous generators. This requires time-intensive solution of the complex machine equations to control the inverter outputs. This paper demonstrates the development of a simple method for real and reactive power control for the inverters. The method is based on simple circuit theory. For a set values of real and reactive power the magnitude and angle of the inverter output voltage is determined. The PWM technique can be applied to the inverters in order to maintain the calculated operating point. The results obtained from the proposed method have been compared with those obtained from a detailed method. The agreement proves the accuracy of the new technique.
Densification of wood veneers combined with oil-heat treatment. Part II: Hygroscopicity and mechanical properties

In an effort to achieve high mechanical performance and improved dimensional stability, densification combined with . was determined. OHT at 180, 200, and 220ºC for 1, 2, and 3 hours was applied to densified Aspen (Populus tremuloides) veneers. OHT was found to be an efficient treatment to reduce the hygroscopicity of densified aspen veneers, although OHT had a negative impact on Brinell hardness. However, due to the contribution of densification, the hardness of oil-heat treated veneers was still two to three times higher than that of non-densified veneers. Similar results were found for tensile strength. Bending strength increased slightly at low OHT temperature, and then decreased at high temperature. Bending strength of oil-heat treated densified veneer samples was higher than that of non-densified ones. No significant effect of OHT was found on tensile MOE, but bending MOE increased after OHT. Compared to OHT duration, OHT temperature had a larger impact on densified wood hygroscopicity and mechanical properties.
The capacity for understanding mental states - the reflective self in parent and child and its significance for security of attachment

Epidemiologists and psychoanalysts have been equally concerned about the intergenera-tional concordance of disturbed patterns of attachment. Mary Main's introduction of the Adult Attachment Interview (AAI) has provided the field with an empirical tool for examining the concordance of parental and infant attachment patterns. In the context of a prospective study of the influence of parental patterns of attachment assessed before the birth of the first child upon the child's pattern of attachment to that parent at 1 year and at 18 months, the Anna Freud Centre—University College London Parent-Child Project reported a significant level of concordance between parental security and the infant's security with that parent. In the context of this study, a new measure, aiming to assess the parent's capacity for understanding mental states, was developed and is reported on in this paper. The rating of Reflective-Self Function, based upon AAI transcripts, correlated significantly with infant security classification based on Strange Situation assessments. The philosophical background and clinical importance of the measure are discussed.
A Cooperative Intrusion Detection System Framework for Cloud Computing Networks

Cloud computing provides a framework for supporting end users easily attaching powerful services and applications through Internet. To provide secure and reliable services in cloud computing environment is an important issue. One of the security issues is how to reduce the impact of denial-of-service (DoS) attack or distributed denial-of-service (DDoS) in this environment. To counter these kinds of attacks, a framework of cooperative intrusion detection system (IDS) is proposed. The proposed system could reduce the impact of these kinds of attacks. To provide such ability, IDSs in the cloud computing regions exchange their alerts with each other. In the system, each of IDSs has a cooperative agent used to compute and determine whether to accept the alerts sent from other IDSs or not. By this way, IDSs could avoid the same type of attack happening. The implementation results indicate that the proposed system could resist DoS attack. Moreover, by comparison, the proposed cooperative IDS system only increases little computation effort compared with pure Snort based IDS but prevents the system from single point of failure attack.
CXCR4 Controls Ventral Migration of Sympathetic Precursor Cells

The molecular mechanisms that sort migrating neural crest cells (NCCs) along a shared pathway into two functionally discrete structures, the dorsal root ganglia and sympathetic ganglia (SGs), are unknown. We report here that this patterning is attributable in part to differential expression of the chemokine receptor, CXCR4. We show that (1) a distinct subset of ventrally migrating NCCs express CXCR4 and this subset is destined to form the neural core of the sympathetic ganglia, and (2) the CXCR4 ligand, SDF-1, is a chemoattractant for NCCs in vivo and is expressed adjacent to the future SGs. Reduction of CXCR4 expression in NCCs disrupts their migration toward the future SGs, whereas overexpression of CXCR4 in non-SG-destined NCCs induces them to migrate aberrantly toward the SGs. These data are the first to demonstrate a major role for chemotaxis in the patterning of NCC migration and demonstrate the neural crest is composed of molecularly heterogeneous cell populations.
Spectral tilt as an acoustic correlate to pharyngealisation in Jordanian and Moroccan Arabic

The aim of this study is to evaluate the role of spectral tilt, alongside the traditionally looked at frequencies of the first two formants, in describing the acoustic characteristics of pharyngealisation in Jordanian and Moroccan Arabic. Twenty male speakers (10 per dialect) produced vowels in each dialect preceded by /d dʕ/. Normalised spectral tilt results show an overall lowered values for voice quality correlates, e.g., *H1-*H2, *H1-*A1, *H1-*A2, *H1*A3, and [−ATR] correlate *A1-*A2, and an overall raised values for *A1-*A3 and *A2-*A3 in the pharyngealised context. Results for the former group suggest a tense voice caused by the larynx being raised [24, 26, 28, 35], whereas in the latter suggest a higher energy component close to the F2-F3 region [16]. High classification rates were obtained and showed that spectral tilt was able to distinguish pharyngealised vs non-pharyngealised well, advocating its importance as an acoustic cue for pharyngealisation in Arabic.
Psychopathology of Sex Offenders

Although male sex offenders have been the focus of numerous empirical investigations, there exists little data examining the characteristics of female sex offenders. This study examined personality profiles of incarcerated male and female sex offenders by utilizing Latent Profile Analysis (LPA) to categorize offenders based on their responses on the Personality Assessment Inventory (PAI). Building on previous research (see Turner, Miller, Henderson, 2008), the current sample of male and female sex offenders is best described using a 4-class model: Moderate Defensiveness, Elevated Alcohol and Drug Use, Moderate Psychopathology, and Elevated Psychopathology. Analyzing covariates and group membership, results indicated that male sex offenders were more likely to be included in the Elevated Drug and Alcohol group, whereas the female sex offenders were more likely to be included in the Moderate or Elevated Psychopathology group. Additional results of psychopathology and gender difference, as well as treatment implications are discussed.
Measurement Methodologies for Assessing the Glycolysis Effect in the Discrimination and Therapy of Brain Gliomas

Primary brain tumors refer to those developing from the various types of cells that compose the brain. Gliomas represent about 50% of all primary brain tumors and include a variety of different histological tumor types and malignancy grades. The World Health Organization WHO classifies gliomas into four histological types and four grades. The goal of molecular classification using advanced pattern recognition tools is to identify subgroups of tumors with distinct biological and clinical features and initiate the challenge of classifying complex gliomas of similar histology and malignancy status into distinct categories. The aim of this paper is to i present the measurement procedures and analysis methodologies, ii summarize the currently available knowledge related to the utilization of 'omics' measurements in the discrimination of brain gliomas, and iii provide a scientific basis for future medical practice in the discrimination and treatment of brain gliomas based specifically on the metabolic process of glycolysis. In particular, the paper explores the idea of the glycolysis pathway as a critical concept for the development of therapeutic strategies for brain gliomas.