Miracle-dz/UniFAR-FTUs huggingface dataset:
Understanding the Mechanisms of Change in a Lifestyle Intervention for Older Adults

Purpose of the Study This study investigated the mechanisms of change underlying an activity-based lifestyle intervention, an occupational therapy program aimed at promoting healthy habits, and routines in older adults. We examined two activity-relevant factors as potential mediators linking the intervention to reduced symptoms of depression: activity frequency and global perceptions of activity significance. Social connections and perceived control were assessed to understand how activity-related factors relate to reduced symptoms of depression. Design and Methods The sample consisted of 460 multiethnic community-dwelling older adults aged 60-95 years. Participants were randomly assigned to a 6-month lifestyle redesign intervention (n = 232) or a no-treatment control (n = 228) condition. After the 6-month period, 360 individuals completed post-testing. Latent change score models were used to represent changes from baseline over the experimental interval. Structural equation models were applied to examine the indirect effects of the intervention on reduced depressive symptoms. Results The results demonstrated significant indirect effects from intervention receipt to decreased depressive symptoms via increased activity frequency and activity significance. Higher activity frequency was linked to fewer depressive symptoms via heightened social connections, whereas increased activity significance was associated with fewer depressive symptoms via enhanced perceived control. Implications The results support basic principles of occupational therapy by highlighting important mediating roles of activity frequency and activity significance in reducing depressive symptoms. Understanding of these change mechanisms can help optimize activity-centered interventions to reduce depressive symptoms.
A SAS-6-Like Protein Suggests that the Toxoplasma Conoid Complex Evolved from Flagellar Components

ABSTRACT SAS-6 is required for centriole biogenesis in diverse eukaryotes. Here, we describe a novel family of SAS-6-like (SAS6L) proteins that share an N-terminal domain with SAS-6 but lack coiled-coil tails. SAS6L proteins are found in a subset of eukaryotes that contain SAS-6, including diverse protozoa and green algae. In the apicomplexan parasite Toxoplasma gondii, SAS-6 localizes to the centriole but SAS6L is found above the conoid, an enigmatic tubulin-containing structure found at the apex of a subset of alveolate organisms. Loss of SAS6L causes reduced fitness in Toxoplasma. The Trypanosoma brucei homolog of SAS6L localizes to the basal-plate region, the site in the axoneme where the central-pair microtubules are nucleated. When endogenous SAS6L is overexpressed in Toxoplasma tachyzoites or Trypanosoma trypomastigotes, it forms prominent filaments that extend through the cell cytoplasm, indicating that it retains a capacity to form higher-order structures despite lacking a coiled-coil domain. We conclude that although SAS6L proteins share a conserved domain with SAS-6, they are a functionally distinct family that predates the last common ancestor of eukaryotes. Moreover, the distinct localization of the SAS6L protein in Trypanosoma and Toxoplasma adds weight to the hypothesis that the conoid complex evolved from flagellar components.
Childhood obesity treatment; Effects on BMI SDS, body composition, and fasting plasma lipid concentrations

Objective The body mass index (BMI) standard deviation score (SDS) may not adequately reflect changes in fat mass during childhood obesity treatment. This study aimed to investigate associations between BMI SDS, body composition, and fasting plasma lipid concentrations at baseline and during childhood obesity treatment. Methods 876 children and adolescents (498 girls) with overweight/obesity, median age 11.2 years (range 1.6–21.7), and median BMI SDS 2.8 (range 1.3–5.7) were enrolled in a multidisciplinary outpatient treatment program and followed for a median of 1.8 years (range 0.4–7.4). Height and weight, body composition measured by dual-energy X-ray absorptiometry, and fasting plasma lipid concentrations were assessed at baseline and at follow-up. Lipid concentrations (total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), non-HDL, and triglycerides (TG)) were available in 469 individuals (264 girls). Linear regressions were performed to investigate the associations between BMI SDS, body composition indices, and lipid concentrations. Results At baseline, BMI SDS was negatively associated with concentrations of HDL (p = 6.7*10−4) and positively with TG (p = 9.7*10−6). Reductions in BMI SDS were associated with reductions in total body fat percentage (p<2*10−16) and percent truncal body fat (p<2*10−16). Furthermore, reductions in BMI SDS were associated with improvements in concentrations of TC, LDL, HDL, non-HDL, LDL/HDL-ratio, and TG (all p <0.0001). Changes in body fat percentage seemed to mediate the changes in plasma concentrations of TC, LDL, and non-HDL, but could not alone explain the changes in HDL, LDL/HDL-ratio or TG. Among 81 individuals with available lipid concentrations, who increased their BMI SDS, 61% improved their body composition, and 80% improved their lipid concentrations. Conclusion Reductions in the degree of obesity during multidisciplinary childhood obesity treatment are accompanied by improvements in body composition and fasting plasma lipid concentrations. Even in individuals increasing their BMI SDS, body composition and lipid concentrations may improve.
Efficient Data Mining for Path Traversal Patterns

The authors explore a new data mining capability that involves mining path traversal patterns in a distributed information-providing environment where documents or objects are linked together to facilitate interactive access. The solution procedure consists of two steps. First, they derive an algorithm to convert the original sequence of log data into a set of maximal forward references. By doing so, one can filter out the effect of some backward references, which are mainly made for ease of traveling and concentrate on mining meaningful user access sequences. Second, they derive algorithms to determine the frequent traversal patterns-i.e., large reference sequences-from the maximal forward references obtained. Two algorithms are devised for determining large reference sequences; one is based on some hashing and pruning techniques, and the other is further improved with the option of determining large reference sequences in batch so as to reduce the number of database scans required. Performance of these two methods is comparatively analyzed. It is shown that the option of selective scan is very advantageous and can lead to prominent performance improvement. Sensitivity analysis on various parameters is conducted.
Advances in receptor conformation research: the quest for functionally selective conformations focusing on the β2‐adrenoceptor

Seven‐transmembrane receptors, also called GPCRs, represent the largest class of drug targets. Upon ligand binding, a GPCR undergoes conformational rearrangement and thereby changes its interaction with effector proteins including the cognate G‐proteins and the multifunctional adaptor proteins, β‐arrestins. These proteins, by initiating distinct signal transduction mechanisms, mediate one or several functional responses. Recently, the concept of ligand‐directed GPCR signalling, also called functional selectivity or biased agonism, has been proposed to explain the phenomenon that chemically diverse ligands exhibit different efficacies towards the different signalling pathways of a single GPCR, and thereby act as functionally selective or ‘biased’ ligands. Current concepts support the notion that ligand‐specific GPCR conformations are the basis of ligand‐directed signalling. Multiple studies using fluorescence spectroscopy, X‐ray crystallography, mass spectroscopy, nuclear magnetic resonance spectroscopy, single‐molecule force spectroscopy and other techniques have provided the evidence to support this notion. It is anticipated that these techniques will ultimately help elucidate the structural basis of ligand‐directed GPCR signalling at a precision meaningful for structure‐based drug design and how a specific ligand molecular structure induces a unique receptor conformation leading to biased signalling. In this review, we will summarize recent advances in experimental techniques applied in the study of functionally selective GPCR conformations and breakthrough data obtained in these studies particularly those of the β2‐adrenoceptor.
Toward a Quality-Controlled and Accessible Pitzer Model for Seawater and Related Systems

We elaborate the need for a quality-controlled chemical speciation model for seawater and related natural waters, work which forms the major focus of SCOR Working Group 145. Model development is based on Pitzer equations for the seawater electrolyte and trace components. These equations can be used to calculate activities of dissolved ions and molecules and, in combination with thermodynamic equilibrium constants, chemical speciation. The major tasks to be addressed are ensuring internal consistency of the Pitzer model parameters (expressing the interactions between pairs and triplets of species, which ultimately determines the calculated activities), assessing uncertainties, and identifying important data gaps that should be addressed by new measurements. It is recognised that natural organic matter plays an important role in many aquatic ecosystems, and options for including this material in a Pitzer-based model are discussed. The process of model development begins with the core components which include the seawater electrolyte and the weak acids controlling pH. This core model can then be expanded by incorporating additional chemical components, changing the standard seawater composition and/or broadening the range of temperature and pressure, without compromising its validity. Seven important areas of application are identified: open ocean acidification; micro-nutrient biogeochemistry and geochemical tracers; micro-nutrient behaviour in laboratory studies; water quality in coastal and estuarine waters; cycling of nutrients and trace metals in pore waters; chemical equilibria in hydrothermal systems; brines and salt lakes.
Availability of Information About Lifestyle for Cancer Survivors in England: A Review of Statutory and Charitable Sector Organizations and Cancer Centers

Background Health behavior change following a cancer diagnosis has the potential to improve long-term outcomes. However, many patients do not receive professional advice about lifestyle and are therefore increasingly using the Internet to seek further information. The statutory and charitable sectors and cancer centers all play an important role in the provision of information and have been found to be favored by cancer survivors searching for information. However, to date there has been no systematic evaluation of the lifestyle information available online for cancer survivors. Objective The purpose of this review was to identify the lifestyle information provided for cancer survivors by statutory and charitable sector organizations and cancer centers in the United Kingdom. We aimed to identify information on tobacco, physical activity, diet, weight, and alcohol designed for people who have been diagnosed with breast, prostate, or colorectal cancer. Methods The National Health Service (NHS) website was the focus of the search for information provided by the statutory sector. Cancer centers were identified from the Organization of European Cancer Institutes and an Internet search, and charitable sector organizations were identified by searching the Charity Commission database. The three largest generic, breast, prostate, and colorectal cancer charitable organizations were included. A systematic search of the organizations was conducted to identify lifestyle information for cancer survivors. Results Ten organizations had some lifestyle information for cancer survivors on their websites. The Christie NHS Foundation Trust, Macmillan Cancer Support, and Prostate Cancer UK had the most comprehensive guides, covering physical activity, diet, weight management, smoking, and alcohol. The NHS website did not provide any information but had a link to Cancer Research UK’s information about diet. Eight organizations suggested talking to a health professional before making any changes. Conclusions The majority of organizations included in this review would benefit from updating their websites to include adequate information and advice about lifestyle for cancer survivors, or they risk cancer survivors turning to less reliable sources of information. Health professionals should be appropriately trained to deal with questions about lifestyle and to advise cancer survivors about lifestyle changes following their diagnosis.
Twenty-Four-Hour Ambulatory Blood Pressure versus Clinic Blood Pressure Measurements and Risk of Adverse Outcomes in Children with CKD.

BACKGROUND and objectives Our objective was to determine whether clinic BPs (taken at either a single visit or two sequential visits) are inferior to ambulatory BPs in their ability to discriminate risk of adverse outcomes in children with CKD. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS We included 513 participants of the CKD in Children Study who had clinic BPs and 24-hour ambulatory BP monitoring performed during similar timeframes. Predictors of interest were systolic BPs taken at a single visit or two repeated visits within a 1-year period compared with mean wake and sleep systolic ambulatory BPs. Outcomes were left ventricular hypertrophy and ESKD. We determined the ability for each BP parameter to provide risk discrimination using c statistics. RESULTS During mean follow-up of 3.5 years, 123 participants developed ESKD. In cross-sectional unadjusted analysis, every 0.1 increase in systolic BP index was associated with a 2.0 times higher odds of left ventricular hypertrophy (95% confidence interval, 1.5 to 2.8) by clinic BPs versus 1.8 times higher odds (95% confidence interval, 1.3 to 2.4) by ambulatory wake BP. The c statistic was highest for clinic BP (c=0.65; 95% confidence interval, 0.58 to 0.73) but similar to ambulatory wake BP (c=0.64; 95% confidence interval, 0.57 to 0.71) for the discrimination of left ventricular hypertrophy. In longitudinal unadjusted analysis, every 0.1 increase in systolic BP index was associated with a higher risk of ESKD using repeated clinic (hazard ratio, 1.5; 95% confidence interval, 1.3 to 1.8) versus ambulatory wake BP (hazard ratio, 1.6; 95% confidence interval, 1.3 to 2.0). Unadjusted c statistics were the same for wake (c=0.61; 95% confidence interval, 0.56 to 0.67) and clinic systolic BPs (c=0.61; 95% confidence interval, 0.55 to 0.66) for discriminating risk of ESKD. CONCLUSIONS Clinic BPs taken in a protocol-driven setting are not consistently inferior to ambulatory BP in the discrimination of BP-related adverse outcomes in children with CKD.
Computer investigation of a sine and cosine based phase-locked loop for single phase grid connected inverter

This paper presents a new approach for PLL for synchronization with the public grid phase and frequency of grid-connected single phase inverter. The approach uses trigonometric transformations of the inverter output voltage and the grid voltage. The proposed mathematical model is then studied by means of computer simulation for different voltage amplitudes of the grid voltage as well as the inverter output voltage.
What is the skill of ocean tracers in reducing uncertainties about ocean diapycnal mixing and projections of the Atlantic Meridional Overturning Circulation

[1] Current projections of the oceanic response to anthropogenic climate forcings are uncertain. Two key sources of these uncertainties are (1) structural errors in current Earth system models and (2) imperfect knowledge of model parameters. Ocean tracer observations have the potential to reduce these uncertainties. Previous studies typically consider each tracer separately, neglect potentially important statistical properties of the system, or use methods that impose rather daunting computational demands. Here we extend and improve upon a recently developed approach using horizontally averaged vertical profiles of chlorofluorocarbon (CFC-11), radiocarbon (Δ14C), and temperature (T) observations to reduce model parametric and structural uncertainties. Our method estimates a joint probability density function, which considers cross-tracer correlations and spatial autocorrelations of the errors. We illustrate this method by estimating two model parameters related to the vertical diffusivity, the background vertical diffusivity, and the upper Southern Ocean mixing. We show that enhancing the upper Southern Ocean mixing in the model improves the representations of ocean tracers and improves the hindcasts of the Atlantic Meridional Overturning Circulation (AMOC). The most probable value of the background vertical diffusivity in the pelagic pycnocline is between 0.1 and 0.2 cm2 s−1. According to the statistical method, observations of Δ14C reduce the uncertainty about the background vertical diffusivity mostly followed by CFC-11 and T. Using all three tracers jointly reduces the model uncertainty by 40%, more than each tracer individually. Given several important caveats, we illustrate how the reduced model parametric uncertainty improves probabilistic projections of the AMOC.
Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T Cells

T helper cell type 1 (Th1) and 2 (Th2) are central to immune regulation. However, no stable cell surface marker capable of distinguishing and separating these two subsets of CD4+ cells has yet been found. Using differential display PCR, we have identified a gene encoding a cell membrane bound molecule, originally designated ST2L, T1, DER4, or Fit, expressed constitutively and stably on the surface of murine Th2s, but not Th1s even after stimulation with a range of immunological stimuli. Antibody against a peptide derived from ST2L strongly and stably labeled the surface of cloned Th2s but not Th1s, and Th2s but not Th1s derived from naive T cells of ovalbumin T cell receptor–α/β transgenic mice. Three-color single cell flow cytometric analysis shows that cell surface ST2L coexpressed with intracellular interleukin (IL)-4, but not with interferon (IFN)-γ. The antibody selectively lysed Th2s in vitro in a complement-dependent manner. In vivo, it enhanced Th1 responses by increasing IFN-γ production and decreasing IL-4 and IL-5 synthesis. It induced resistance to Leishmania major infection in BALB/c mice and exacerbated collagen-induced arthritis in DBA/1 mice. Thus, ST2L is a stable marker distinguishing Th2s from Th1s and is also associated with Th2 functions. Hence, it may be a target for therapeutic intervention.
Separable Reversible Data Hiding in Encrypted Images Based on Two-Dimensional Histogram Modification

An efficient method of completely separable reversible data hiding in encrypted images is proposed. The cover image is first partitioned into nonoverlapping blocks and specific encryption is applied to obtain the encrypted image. Then, image difference in the encrypted domain can be calculated based on the homomorphic property of the cryptosystem. The data hider, who does not know the original image content, may reversibly embed secret data into image difference based on two-dimensional difference histogram modification. Data extraction is completely separable from image decryption; that is, data extraction can be done either in the encrypted domain or in the decrypted domain, so that it can be applied to different application scenarios. In addition, data extraction and image recovery are free of any error. Experimental results demonstrate the feasibility and efficiency of the proposed scheme.
Arf6 and the 5'phosphatase of synaptojanin 1 regulate autophagy in cone photoreceptors

Abnormalities in the ability of cells to properly degrade proteins have been identified in many neurodegenerative diseases. Recent work has implicated synaptojanin 1 (SynJ1) in Alzheimer's disease and Parkinson's disease, although the role of this polyphosphoinositide phosphatase in protein degradation has not been thoroughly described. Here, we dissected in vivo the role of SynJ1 in endolysosomal trafficking in zebrafish cone photoreceptors using a SynJ1‐deficient zebrafish mutant, nrca14. We found that loss of SynJ1 leads to specific accumulation of late endosomes and autophagosomes early in photoreceptor development. An analysis of autophagic flux revealed that autophagosomes accumulate because of a defect in maturation. In addition we found an increase in vesicles that are highly enriched for PI(3)P, but negative for an early endosome marker in nrca14 cones. A mutational analysis of SynJ1 enzymatic domains found that activity of the 5'phosphatase, but not the Sac1 domain, is required to rescue both aberrant late endosomes and autophagosomes. Finally, modulating activity of the PI(4,5)P2 regulator, Arf6, rescued the disrupted trafficking pathways in nrca14 cones. Our study describes a specific role for SynJ1 in autophagosomal and endosomal trafficking and provides evidence that PI(4,5)P2 participates in autophagy in a neuronal cell type.
A Conversational In-Car Dialog System

In this demonstration we present a conversational dialog system for automobile drivers. The system provides a voice-based interface to playing music, finding restaurants, and navigating while driving. The design of the system as well as the new technologies developed will be presented. Our evaluation showed that the system is promising, achieving high task completion rate and good user satisfation.
STRUCTURAL PROPERTIES OF RICE HUSK ASH CONCRETE.

This research was experimentally carried out to investigate the effects of introducing Rice Husk Ash (RHA) as a Partial Replacement of Ordinary Portland Cement (OPC) on the Structural Properties of Concrete. Rice Husk Ash which is an Agro-Waste and known to be a Super Pozzoland have been used for mass concrete and found to have compressive strength ranging from 33-38.4N/mm 2 at replacement percentages of 10-25% in a mix of 1:1.5:3. A further study was carried out on its flexural properties to determine their moduli of rupture as well as its tensile strength characteristics for the determination of cracking, the values obtained at 28days are 3, 2.5 and 2.4N/mm 2 while the tensile strength values are 1.94, 1.17 and 0.91N/mm 2 at replacement percentages of 10%, 20% and 25%. This research has proved that RHA Concrete can be used as a Structural Concrete at suitable replacement percentages. This research therefore is an investigation of the performance of the concrete made of partially replacing OPC with RHA on the structural integrity and properties of RHA concrete.