Miracle-dz/UniFAR-FTUs huggingface dataset:
Particle Debris Generated from Passenger Tires Induces Morphological and Gene Expression Alterations in the Macrophages Cell Line RAW 264.7

Air pollution in the urban environment is a topical subject. Aero-suspended particles can cause respiratory diseases in humans, ranging from inflammation to asthma and cancer. One of the components that is most prevalent in particulate matter (PM) in urban areas is the set of tire microparticles (1–20 μm) and nanoparticles (<1 μm) that are formed due to the friction of wheels with asphalt and are increased in slow-moving areas that involve a lot of braking actions. In this work, we studied the effect that microparticles generated from passenger tires (PTWP, passenger tire wear particles) have in vitro on murine macrophages cells RAW 264.7 at two concentrations of 25 and 100 μg/mL, for 24 and 48 h. In addition to the chemical characterization of the material and morphological characterization of the treated cells by transmission electron microscopy, gene expression analysis with RT-PCR and active protein analysis with Western blotting were performed. Growth curves were obtained, and the genotoxic effect was evaluated with a comet assay. The results indicate that initially, an induction of the apoptotic process is observable, but this is subsequently reversed by Bcl2. No genotoxic damage is present, but mild cellular abnormalities were observed in the treated cells.
Relationship between the Consciousness State and the Default Mode Network in Traumatic Brain Injury.

INTRODUCTION This study investigated the relationship between Coma Recovery Scale-Revised (CRS-R) and the neural networks between the medial prefrontal cortex (mPFC) and precuneus (PCun)/posterior cingulate cortex (PCC) in disorders of consciousness (DOC) patients with a traumatic brain injury (TBI) using diffusion tensor tractography (DTT). MEASURES Twenty-five consecutive patients with TBI admitted to the rehabilitation department of a university hospital were enrolled in this study. The Coma Recovery Scale-Revised (CRS-R) was used to evaluate the consciousness state. The pathway of the neural networks between the mPFC and the PCun (mPFC-PCun DMN)/PCC (mPFC-PCC DMN) were reconstructed using DTT. Fractional anisotropy (FA) and the tract volume (TV) were obtained to assess the diffusion tensor imaging parameters. RESULTS The CRS-R score had strong positive correlations with the FA value and TV of the mPFC-PCun DMN (p < 0.05), while it showed a moderate positive correlation with the TV of the mPFC-PCC DMN (p < 0.05). In addition, the FA value of the mPFC-Pcun DMN showed that it could explain the variability in the CRS-R score. CONCLUSIONS The close correlation was noted between the consciousness state and the mPFC-PCun DMN and mPFC-PCC DMN in DOC patients with TBI. On the other hand, the mPFC-PCun DMN appeared to be more closely correlated with the consciousness state than the mPFC-PCC DMN.
APPL1 Is a Prognostic Biomarker and Correlated with Treg Cell Infiltration via Oxygen-Consuming Metabolism in Renal Clear Cell Carcinoma

Kidney renal clear cell carcinoma (KIRC) is one of the most hazardous tumors in the urinary system. The regulation of oxygen consumption in renal clear cell carcinoma is a consequence of adaptive reprogramming of oxidative metabolism in tumor cells. APPL1 is a signaling adaptor involved in cell survival, oxidative stress, inflammation, and energy metabolism. However, the correlation of APPL1 with regulatory T cell (Treg) infiltration and prognostic value in KIRC remain unclear. In this study, we comprehensively predicted the potential function and prognostic value of APPL1 in KIRC. For KIRC patients, relatively low expression of APPL1 was associated with high degree of metastasis, pathological stage, and shorter overall time or poor prognosis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses suggested that low expression of APPL1 may be adapted to the malignant progression of tumors via affecting oxygen-consuming metabolism. In addition, the expression level of APPL1 was negatively correlated with Treg cell infiltration and chemotherapy sensitivity, which indicated that APPL1 may regulate the tumor immune infiltration and chemotherapy resistance by decrease oxygen-consuming metabolic process in KIRC. Therefore, APPL1 may become one of the important prognostic factors, and it may serve as a candidate prognostic biomarker in KIRC.
Atmospheric multiple equilibria and non‐Gaussian behaviour in model simulations

Multiple equilibria from general circulation models (GCMs) are investigated via Gaussian mixture models. The cross‐validation technique based on bootstrap, used to find the possible number of clusters, is shown to have a tendency to display a bias in the number of clusters when the sample is not statistically independent. The method is shown, however, to give satisfactory results when applied with a strategy based on statistical independence. The mixture model is successfully applied to a few dynamical systems and data from the UK Universities Global Atmospheric Modelling Programme (UGAMP) GCM. The same analysis is then carried out and applied to the wintertime 500 mb geopotential height field taken from a 50‐year run of the Hadley Centre HadAM3 GCM. Three regions, the North Pacific‐American, the North Atlantic, and the northern hemisphere are investigated for nonlinear circulation regimes. It is shown that the model displays robust regime behaviour over the North Pacific‐American sector, while only marginal regime behaviour is observed over the North Atlantic, and no trace of hemispheric regimes.
List Improper Colourings of Planar Graphs

A graph G is m-choosable with impropriety d, or simply (m, d)*-choosable, if for every list assignment L, where [mid ]L(v)[mid ][ges ]m for every v∈V(G), there exists an L-colouring of G such that each vertex of G has at most d neighbours coloured with the same colour as itself. We show that every planar graph is (3, 2)*-choosable and every outerplanar graph is (2, 2)*-choosable. We also propose some interesting problems about this colouring.
Nanolaser spectroscopy for studying novel biomaterials

Recent semiconductor technology has reduced the size of a laser to the size of a biological cell or even a virus particle. By integrating these ultra small lasers with biological systems, it is possible to create micro-electrical mechanical systems (MEMS) devices that are rapidly finding new applications for chemical analysis, molecular detection, and health care.1-5 One is a nanolaser device that confines intense light into an extremely small interaction volume.6-10 The nanolaser has been integrated with a microfluidic chip and applied to assess novel biomaterials, cells, and organelles. Importantly, these biomaterials can be analyzed without time delays or difficulties associated with chemical fixing or fluorescent markers. With these advantages, nanolaser spectroscopy represents a powerful tool for the rapid analysis of bioparticles such as cells, organelles, vesicles, virions, and other bioparticles.
High Resolution Multiplex Confocal Imaging of the Neurovascular Unit in Health and Experimental Ischemic Stroke

The neurovascular unit (NVU) is an anatomical group of cells that establishes the blood–brain barrier (BBB) and coordinates cerebral blood flow in association with neuronal function. In cerebral gray matter, cellular constituents of the NVU include endothelial cells and associated pericytes, astrocytes, neurons, and microglia. Dysfunction of the NVU is a common feature of diseases that affect the CNS, such as ischemic stroke. High-level evaluation of these NVU changes requires the use of imaging modalities that can enable the visualization of various cell types under disease conditions. In this study, we applied our confocal microscopy strategy using commercially available labeling reagents to, for the first time, simultaneously investigate associations between endothelial cells, the vascular basal lamina, pericytes, microglia, astrocytes and/or astrocyte end-feet, and neurites in both healthy and ischemic brain tissue. This allowed us to demonstrate ischemia-induced astrocyte activation, neurite loss, and microglial migration toward blood vessels in a single confocal image. Furthermore, our labeling cocktail enabled a precise quantification of changes in neurites and astrocyte reactivity, thereby showing the relationship between different NVU cellular constituents in healthy and diseased brain tissue. The application of our imaging approach for the simultaneous visualization of multiple NVU cell types provides an enhanced understanding of NVU function and pathology, a state-of-the-art advancement that will facilitate the development of more effective treatment strategies for diseases of the CNS that exhibit neurovascular dysfunction, such as ischemic stroke.
Kirchhoof's formula, its vector analogue, and other field equivalence theorems

1. lntroduction One of the purposes of this symposium is to dissuss some of the theoretical difficulties involved in the solution of electromagnetic problems. For this reason I believe that the treatment of diffraction problems is an appropriate topic. In particular, the problem of radiation from a horn (acoustic or electromagnetic) has been treated approximately with the aid of one of several formulas suggested by Huygens' physical idea about wave propagation. These formulas are not equally powerful, do not always give equally good approximations, and do not inspire the same a pm'ori confidence in the results. Of course, if no approximations were made in the formulas, it would not matter which formula was used since then the result would always be exact. However, approximations are unavoidable except when the answer is already known and when there is no need for any formula. To understand how Huygens' physical assumption that the conditions at the front of a wave determine the subsequent wave motion suggests various formulas, let us consider a source first in an infinite homogeneous medium and then inside a perfectly rigid horn. In the first case the wavefronts are closed surfaces surrounding the source. In the second case, the wavefronts are open surfaces sliding along the walls of the horn until they reach the aperture. Eventually , the wavefronts will become closed surfaces surrounding the horn as well as the source. Kirchhoff derived an explicit formula for the field outside a closed surface (8) in terms of the wave function and its normal derivative on (8) on the assumption that the source is inside (8) and that the medium outside (S) is homogeneous. In the case of the horn Kirchhoff's surface of integration must enclose the horn as well as the source since the horn introduces a discon-tinuity in the medium. This closed surface may be chosen to consist of an " aperture surface " (8.) together with the exterior surface of the horn. In the case of electromagnetic waves the present writer proved another theorem [l, 21 (the " Induction Theorem ") in which the surface of integration is solely the aperture surface (8J. An obvious analogue of this theorem for scalar waves
An Accelerated Forward–Backward Iterative Physical Optics for Electromagnetic Scattering from Rough Sea Surface

Abstract This article proposes to solve the extra-large-scale (e.g., larger than 1,000 square lambda) electric electromagnetic scattering problem on a wind-driven sea surface at a low grazing angle directed against a shore-based radar with an iterative physical optics model. In order to accelerate the convergence of the iterative physical optics model and improve its robustness, the forward–backward methodology and its modification with under-relaxation iteration are developed first to simulate the rough sea surface scattering. Meanwhile, according to the scattering characteristics of rough sea surface, the local iteration methodology and the fast far-field approximation in the matrix-vector product are proposed to reduce greatly the computational complexity. Through these techniques, this model can solve effectively the extra-large-scale electric scattering problem from the randomly rough sea surfaces.
COVID-19 Vaccine Hesitancy among New Jersey Teachers and Impacts of Vaccination Information Dissemination

Vaccine hesitancy continues to be prevalent in the United States, especially in relation to the COVID-19 vaccines and its boosters, which have been made increasingly available for public use as the pandemic has progressed. There continues to be concern surrounding the safety and health of secondary or high school education professionals as they transition back to in-person learning and working opportunities. The present study highlights how information dissemination regarding the COVID-19 vaccine has varied among New Jersey secondary or high school teachers throughout the pandemic. The survey was completed online through the PsychData platform by 269 participants between March and July 2022. Participants received the opportunity to complete the survey via email. Afterwards, data were exported and analyzed using Microsoft Excel and SAS 9.4 Analytics Software and stratified by various clinical and demographic-based variables. While trusted agencies and media outlets identified by participants varied, most participants identified the Centers for Disease Control and Prevention (65.4%), primary care providers (37.5%), and state health departments (28.6%) as their top trusted sources for information related to COVID-19 vaccines. Overall, COVID-19 vaccination advocacy and educational efforts should continue across the state of New Jersey and elsewhere, especially as more variants emerge and boosters become available.
Identification and Comparative Expression Profiles of Candidate Olfactory Receptors in the Transcriptomes of the Important Egg Parasitoid Wasp Anastatus japonicus Ashmead (Hymenoptera: Eupelmidae)

Anastatus japonicus Ashmead is an egg parasitoid wasp important for the biological control of fruit crop pests. The olfaction of parasitoids is crucial to searching for host pests in fruit crops. In this study, we sequenced and analyzed the antennal and abdominal transcriptomes of A. japonicus to better understand the olfactory mechanisms in this species. A total of 201 putative olfactory receptor genes were identified, including 184 odorant receptors (ORs) and 17 ionotropic receptors (IRs). Then, we assayed the tissue-specific and sex-biased expression profiles of those genes based on the transcriptional levels. In total, 165 ORs and 15 IRs had upregulated expression in the antennae. The expression levels of 133 ORs, including odorant receptor co-receptor (AjapORco), and 10 IRs, including AjapIR8a, were significantly different between the female and male antennae. Our results provide valuable information for further studies on the molecular mechanisms of the olfactory system in A. japonicus.
A Global Interdisciplinary Service-Learning Project to Develop Cultural Humility in Educators of Children with Autism and Developmental Disabilities

A four-week global interdisciplinary service-learning project to Botswana, Africa was developed to increase cultural humility and cross cultural communication skills in 12 current and future educators of children with autism and developmental disabilities. Participants worked alongside peer educators in eight different special education units across different regions of Botswana to create curricular activities that can be used in classrooms in both countries. Instruction in Botswana education, culture, language, history, and traditions was provided along with immersion in daily Botswana life. The present study examines the impact of these experiences on participant growth of cultural humility and cross-cultural communication skills. Results indicated increases in cultural humility for all participants, with the greatest increases for the stages of integration and intercultural communication. Individual growth varied based on factors such as previous experiences, chronological age, and flexibility. In this paper, we discuss details of the project, results, limitations, and implications for practice. Abstract in Setswana Lenaneo la beke tse nne go ya Botswana, le le akaretsang mafatshefatshe, ebile ele la dikitso tse di farologanyeng, le ne la bopiwa go oketsa kitso ka ngwao ya Setswana le ka ha go buisanwang ka teng mo ngwaong. Kitso tse tsa ngwao, di ne di itebagantse le barutabana bale lesome le bobedi. Barutabana ba, ene ele ba jaanong le ba isago, ba ruta bana ba ba nang le autism le bogole jo bo farologaneng. Batsaya karolo ba ne ba bereka le barutabana mo makalaneng a a farologanyeng a le boroba bobedi ko Botswana, a a lebaganeng le dithuto tsa autism le bana ba ba nang le bogole. Ba dira jaana go bopa ditsamaiso tsa thuto tse di ka dirisiwang mo dikolong tsa mafatshe oo mabedi. Batsaya karolo ba ne ba tlhatlhelelwa ka tsamaiso ya thuto, ngwao, puo, ditso le tumelo mo Botswana, le gore di amana jang le matshelo a Batswana a tsatsi le letsatsi. Dipatlisiso tse di leka go kala maduo a phetogo maikutlo a batsaakarolo mo go godiseng kitso ka ngwao le dipuisano ka yone mo Botswana. Maduo a dipatlisiso a supile fa barutabana ba nnile le kgolo mo go tlhaloganyeng ngwao, go gola mo go tona e nnile go tlhaloganya dingwao tsa mafatshe ka bobedi. Se se raya gore lenaneo le, le nnile botlhokwa thata mo go bone. Go gola ga barutabana ka bongwe ka bongwe go ne go ikaegile thata ka maitemogelo a bone pele ga ba ya Botswana, dingwaga tsa bone le ka ha ba amogelang diemo tse di farologanyeng ka teng. Mo mokwalong o, re ala ka botlalo ka ha lenaneo le tsamaileng ka teng, maduo le ditlhaelo/dikgwetlho le ka fa lenaneo le ka dirisiwang ka teng go ya pele.
The Impact of Particle Size and Surface Treatment of Zirconia Suspension for Photocuring Additive Manufacturing

To prepare a photocurable ceramic suspension for use in commercialized additive manufacturing equipment, the effects of the rheological properties of zirconia particles added to a binder, and the presence or absence of a silane coupling agent on the particles was evaluated. To this end, three experimental groups (ZSs, ZMs, ZLs) and three control groups (ZS, ZM, ZL) were designed depending on the size of the underlying zirconia particles. The test-group zirconia suspensions were prepared through silanization, which was not applied to the control-group suspensions. Depending on the particle size, viscosity differences between the test and control groups were 16,842, 18,623, and 12,303 mPa·s, respectively. Compared to the other groups, the viscosity of the ZLs group suspension decreased by 70.98–88.04%. This confirmed that the viscosity of the suspensions was affected by the particle size and the presence of silane coating. The dispersion stability of the zirconia suspensions was evaluated over 20 days. A sedimentation test confirmed that the sedimentation rate of the ZLs group was slower than those of the other groups. This study aimed to optimize the suspension manufacturing method to effectively be utilized in further commercializing zirconia three-dimensional (3D) printing and could also help to develop various medical applications.
IgG N-Glycosylation Is Altered in Coronary Artery Disease

Coronary artery disease (CAD) is the most common cardiovascular disease (CVD), and previous studies have shown a significant association between N-glycosylation, a highly regulated posttranslational modification, and the development of atherosclerotic plaques. Our aim was to determine whether the N-glycome of immunoglobulin G (IgG) is associated with CAD, as N-glycans are known to alter the effector functions of IgG, which may enhance the inflammatory response in CAD. Therefore, in this study, we isolated IgG from subjects with coronary atherosclerosis (CAD+) and from subjects with clean coronaries (CAD−). The purified IgGs were denatured and enzymatically deglycosylated, and the released and fluorescently labelled N-glycans were analysed by ultra-high performance liquid chromatography based on hydrophilic interactions with fluorescence detection (HILIC-UHPLC-FLR). Sex-stratified analysis of 316 CAD− and 156 CAD+ cases revealed differences in IgG N-glycome composition. The most notable differences were observed in women, where the presence of sialylated N-glycan structures was negatively associated with CAD. The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD.
Surrogate Modelling for Oxygen Uptake Prediction Using LSTM Neural Network

Oxygen uptake (V˙O2) is an important metric in any exercise test including walking and running. It can be measured using portable spirometers or metabolic analyzers. Those devices are, however, not suitable for constant use by consumers due to their costs, difficulty of operation and their intervening in the physical integrity of their users. Therefore, it is important to develop approaches for the indirect estimation of V˙O2-based measurements of motion parameters, heart rate data and application-specific measurements from consumer-grade sensors. Typically, these approaches are based on linear regression models or neural networks. This study investigates how motion data contribute to V˙O2 estimation accuracy during unconstrained running and walking. The results suggest that a long short term memory (LSTM) neural network can predict oxygen consumption with an accuracy of 2.49 mL/min/kg (95% limits of agreement) based only on speed, speed change, cadence and vertical oscillation measurements from an inertial navigation system combined with a Global Positioning System (INS/GPS) device developed by our group, worn on the torso. Combining motion data and heart rate data can significantly improve the V˙O2 estimation resulting in approximately 1.7–1.9 times smaller prediction errors than using only motion or heart rate data.
(LF) ‐ Spaces and Tensor Products

For a countable inductive limit of metrizable locally convex spaces, a condition (M) (in the sense of Retakh) by operators is introduced and the incidence of this property for the inter‐chageability of (LF)‐spaces and tensor products with a Banach space is discussed. Examples are given in the setting of weighted (LF)‐spaces of continuoua functions, Köthe (LF)‐sequence spaces, weighted (LB)‐spaces of holomorphic functiom on balanced domains.
Improvement of Left Ventricular Global Longitudinal Strain after 6-Month Therapy with GLP-1RAs Semaglutide and Dulaglutide in Type 2 Diabetes Mellitus: A Pilot Study

(1) Background: Glucagone-Like Peptide-1 Receptor Agonists (GLP-1 RAs) (GLP-1 RAs) are incretine-based medications recommended in the treatment of type 2 Diabetes Mellitus (DM2) with atherosclerotic cardiovascular disease (ASCVD) or high or very high cardiovascular (CV) risk. However, knowledge of the direct mechanism of GLP-1 RAs on cardiac function is modest and not yet fully elucidated. Left ventricular (LV) Global Longitudinal Strain (GLS) with Speckle Tracking Echocardiography (STE) represents an innovative technique for the evaluation of myocardial contractility. (2) Methods: an observational, perspective, monocentric study was conducted in a cohort of 22 consecutive patients with DM2 and ASCVD or high/very high CV risk, enrolled between December 2019 and March 2020 and treated with GLP-1 RAs dulaglutide or semaglutide. The echocardiographic parameters of diastolic and systolic function were recorded at baseline and after six months of treatment. (3) Results: the mean age of the sample was 65 ± 10 years with a prevalence of the male sex (64%). A significant improvement in the LV GLS (mean difference: −1.4 ± 1.1%; p value < 0.001) was observed after six months of treatment with GLP-1 RAs dulaglutide or semaglutide. No relevant changes were seen in the other echocardiographic parameters. (4) Conclusions: six months of treatment with GLP-1 RAs dulaglutide or semaglutide leads to an improvement in the LV GLS in subjects with DM2 with and high/very high risk for ASCVD or with ASCVD. Further studies on larger populations and with a longer follow-up are warranted to confirm these preliminary results.