Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of injection site on cutaneous basophil hypersensitivity response to phytohemagglutinin.

Cutaneous basophil hypersensitivity response to phytohemagglutinin (PHA) is frequently used as a measure of cell-mediated immune response in chickens. Several anatomical sites have been used to measure this response. No correlation between response level at different sites, however, has been reported. This study compares responses to PHA by using two different injection sites (wing web and interdigital skin of the foot) in White Leghorn chickens. Response at each individual site was comparable whether the bird received a PHA injection at that site alone or at both sites. Correlations between responses at the two sites were not different from zero [-. 1733 (P = .44)]. These data indicate that caution must be exercised in comparing cell-mediated immune responses when different PHA injection sites have been used to assess response.
Ric-8A, a G protein chaperone with nucleotide exchange activity induces long-range secondary structure changes in Gα

Cytosolic Ric-8A has guanine nucleotide exchange factor (GEF) activity and is a chaperone for several classes of heterotrimeric G protein α subunits in vertebrates. Using Hydrogen-Deuterium Exchange-Mass Spectrometry (HDX-MS) we show that Ric-8A disrupts the secondary structure of the Gα Ras-like domain that girds the guanine nucleotide-binding site, and destabilizes the interface between the Gαi1 Ras and helical domains, allowing domain separation and nucleotide release. These changes are largely reversed upon binding GTP and dissociation of Ric-8A. HDX-MS identifies a potential Gα interaction site in Ric-8A. Alanine scanning reveals residues crucial for GEF activity within that sequence. HDX confirms that, like G protein-coupled receptors (GPCRs), Ric-8A binds the C-terminus of Gα. In contrast to GPCRs, Ric-8A interacts with Switches I and II of Gα and possibly at the Gα domain interface. These extensive interactions provide both allosteric and direct catalysis of GDP unbinding and release and GTP binding. DOI: http://dx.doi. org/10.7554/eLife.19238. 001
Ffsck

Failures, errors, and bugs can corrupt file systems and cause data loss, despite the presence of journals and similar preventive techniques. While consistency checkers such as fsck can detect corruption and repair a damaged image, they are generally created as an afterthought, to be run only at rare intervals. Thus, checkers operate slowly, causing significant downtime for large scale storage systems. We address this dilemma by treating the checker as a key component of the overall file system, rather than a peripheral add-on. To this end, we present a modified ext3 file system, rext3, to directly support the fast file-system checker, ffsck. Rext3 colocates and self-identifies its metadata blocks, removing the need for costly seeks and tree traversals during checking. These modifications allow ffsck to scan and repair the file system at rates approaching the full sequential bandwidth of the underlying device. In addition, we demonstrate that rext3 generally performs competitively with ext3 and exceeds it in handling random reads and large writes. Finally, we apply our principles to FreeBSD’s FFS file system and its checker, doing so in a lightweight fashion that preserves the file-system layout while still providing some of the performance gains from ffsck.
Transcriptional Regulator ZEB2 Is Essential for Bergmann Glia Development

Bergmann glia facilitate granule neuron migration during development and maintain the cerebellar organization and functional integrity. At present, molecular control of Bergmann glia specification from cerebellar radial glia is not fully understood. In this report, we show that ZEB2 (aka, SIP1 or ZFHX1B), a Mowat–Wilson syndrome-associated transcriptional regulator, is highly expressed in Bergmann glia, but hardly detectable in astrocytes in the cerebellum. The mice lacking Zeb2 in cerebellar radial glia exhibit severe deficits in Bergmann glia specification, and develop cerebellar cortical lamination dysgenesis and locomotion defects. In developing Zeb2-mutant cerebella, inward migration of granule neuron progenitors is compromised, the proliferation of glial precursors is reduced, and radial glia fail to differentiate into Bergmann glia in the Purkinje cell layer. In contrast, Zeb2 ablation in granule neuron precursors or oligodendrocyte progenitors does not affect Bergmann glia formation, despite myelination deficits caused by Zeb2 mutation in the oligodendrocyte lineage. Transcriptome profiling identified that ZEB2 regulates a set of Bergmann glia-related genes and FGF, NOTCH, and TGFβ/BMP signaling pathway components. Our data reveal that ZEB2 acts as an integral regulator of Bergmann glia formation ensuring maintenance of cerebellar integrity, suggesting that ZEB2 dysfunction in Bergmann gliogenesis might contribute to motor deficits in Mowat–Wilson syndrome. SIGNIFICANCE STATEMENT Bergmann glia are essential for proper cerebellar organization and functional circuitry, however, the molecular mechanisms that control the specification of Bergmann glia remain elusive. Here, we show that transcriptional factor ZEB2 is highly expressed in mature Bergmann glia, but not in cerebellar astrocytes. The mice lacking Zeb2 in cerebellar radial glia, but not oligodendrocyte progenitors or granular neuron progenitors, exhibit severe defects in Bergmann glia formation. The orderly radial scaffolding formed by Bergmann glial fibers critical for cerebellar lamination was not established in Zeb2 mutants, displaying motor behavior deficits. This finding demonstrates a previously unrecognized critical role for ZEB2 in Bergmann glia specification, and points to an important contribution of ZEB2 dysfunction to cerebellar motor disorders in Mowat–Wilson syndrome.
Information Visualization for Interactive Information Retrieval

As search tasks move beyond targeted search and into the domain of complex search, a substantial cognitive burden is placed on the searcher to craft and refine their queries, evaluate and explore among the search results, and ultimately make use of what is found. In such cases, information visualization techniques may be leveraged to enable searchers to perceive, interpret, and make sense of the information available throughout the search process. This tutorial will establish the fundamental principles and theories of information visualization, explain how information visualization can support interactive information retrieval, and survey search interfaces from my own research that leverage information visualization techniques. The goal of this tutorial will be to encourage researchers to make informed design decisions for how to integrate information visualization into their own interactive information retrieval projects.
Cytomorphological Patterns of Tubercular Lymphadenitis Revisited

Background: Tubercular lymphadenitis is one of the most common causes of lymph node enlargement in developing countries. Fine needle aspiration cytology (FNAC) plays an important role in diagnosing Tubercular lymphadenitis and prevents unnecessary surgical intervention. Aim: To study the cytomorphological patterns of tubercular lymphadenitis in our study population and correlate the bacillary load by acid fast staining with cytomorphological patterns. Materials and Methods: A total of 150 cases of cytological proven granulomatous lymphadenitis and cases without granuloma, but positive acid fast bacilli (AFB) on Ziehl Neelson (ZN) stain were included in our study. Cytomorphological patterns were categorized into four patterns. Pattern A - Epithelioid granuloma without necrosis, Pattern B - Epithelioid granuloma with necrosis, Pattern C - Necrosis without epithelioid granuloma with neutrophilic infiltrate. Pattern D - with numerous macrophages. Chi-square test was done to correlate cytomorphological pattern and bacillary load. A 2 × 2 analysis was done to test the degree of one hypothesis. A P < 0.05 was considered as significant. Results: Patterns and the number of cases observed are as follows: Pattern A - 29 cases, Pattern B - 84 cases, Pattern C - 34 cases. A distinct D Pattern with abundant foamy macrophages was seen in three immunodeficient patients. Positive ZN staining was seen in 81/150 54% of cases. The cytological patterns of AFB positive cases Pattern A: 6/29 (21%), Pattern B: 46/84 (55%), Pattern C: 25/34 (73.5%), Pattern D: 4/4 (100%) . Conclusion: FNAC is useful in diagnosing tubercular lymphadenitis. Maximum bacillary load was seen in Pattern C. A distinct D Pattern was seen in immunodeficient patients.
Whey Powder: Process Technology and Physical Properties: A Review

The world's production of cheese whey is much in year yielding an important source of environmental pollution. The main application of spray drying in the cheese industry is the further processing of whey. So, this investigates studied characteristics of different whey produced by spray drying. Also discusses spray-drying process of whey and effect of spray-dryer operating parameters, feed flow rate, atomizer type and inlet/outlet air temperature on food powder physical properties such as bulk density, particle size, moisture content, insoluble solids, wett -ability and morphology of powder particls. The results indicated that spray-dried whey is easier to storage, handling and transport and the quality of spray-dried whey is quite dependent on the spray-dryer operating parameters, so the spray-drying condition was the best way to explain the change quality factors of product powders.
A Review of Future and Ethical Perspectives of Robotics and AI

In recent years there has been increased attention on the possible impact of future robotics and AI systems. Prominent thinkers have publicly warned about the risk of a dystopian future when the complexity of these systems progresses further. These warnings stand in contrast to the current state-of-the-art of the robotics and AI-technology. This article reviews work considering both the future potential of robotics and AI systems, and ethical considerations that need to be taken in order to avoid a dystopian future. References to recent initiatives to outline ethical guidelines for both the design of systems and how they should operate are included.
Enhancing Lifestyle Change in Cardiac Patients Through the Do CHANGE System (“Do Cardiac Health: Advanced New Generation Ecosystem”): Randomized Controlled Trial Protocol

Background Promoting a healthy lifestyle (eg, physical activity, healthy diet) is crucial for the primary and secondary prevention of cardiac disease in order to decrease disease burden and mortality. Objective The current trial aims to evaluate the effectiveness of the Do Cardiac Health: Advanced New Generation Ecosystem (Do CHANGE) service, which is developed to assist cardiac patients in adopting a healthy lifestyle and improving their quality of life. Methods Cardiac patients (ie, people who have been diagnosed with heart failure, coronary artery disease, and/or hypertension) will be recruited at three pilot sites (Badalona Serveis Assistencials, Badalona, Spain [N=75]; Buddhist Tzu Chi Dalin General Hospital, Dalin, Taiwan [N=100] and Elisabeth-TweeSteden Hospital, Tilburg, The Netherlands [N=75]). Patients will be assisted by the Do Something Different (DSD) program to change their unhealthy habits and/or lifestyle. DSD has been developed to increase behavioral flexibility and subsequently adopt new (healthier) habits. In addition, patients’ progress will be monitored with a number of (newly developed) devices (eg, Fitbit, Beddit, COOKiT, FLUiT), which will be integrated in one application. Results The Do CHANGE trial will provide us with new insights regarding the effectiveness of the proposed intervention in different cultural settings. In addition, it will give insight into what works for whom and why. Conclusions The Do CHANGE service integrates new technologies into a behavior change intervention in order to change the unhealthy lifestyles of cardiac patients. The program is expected to facilitate long-term, sustainable behavioral change. Trial Registration Clinicaltrials.gov NCT03178305; https://clinicaltrials.gov/ct2/show/NCT03178305 (Archived by WebCite at http://www.webcitation.org/6wfWHvuyU).
Intentional query suggestion: making user goals more explicit during search

The degree to which users' make their search intent explicit can be assumed to represent an upper bound on the level of service that search engines can provide. In a departure from traditional query expansion mechanisms, we introduce Intentional Query Suggestion as a novel idea that is attempting to make users' intent more explicit during search. In this paper, we present a prototypical algorithm for Intentional Query Suggestion and we discuss corresponding data from comparative experiments with traditional query suggestion mechanisms. Our preliminary results indicate that intentional query suggestions 1) diversify search result sets (i.e. it reduces result set overlap) and 2) have the potential to yield higher click-through rates than traditional query suggestions.
Stateful requirements monitoring for self-repairing socio-technical systems

Socio-technical systems consist of human, hardware and software components that work in tandem to fulfill stakeholder requirements. By their very nature, such systems operate under uncertainty as components fail, humans act in unpredictable ways, and the environment of the system changes. Self-repair refers to the ability of such systems to restore fulfillment of their requirements by relying on monitoring, reasoning, and diagnosing on the current state of individual requirements. Self-repair is complicated by the multi-agent nature of socio-technical systems, which demands that requirements monitoring and self-repair be done in a decentralized fashion. In this paper, we propose a stateful requirements monitoring approach by maintaining an instance of a state machine for each requirement, represented as a goal, with runtime monitoring and compensation capabilities. By managing the interactions between the state machines, our approach supports hierarchical goal reasoning in both upward and downward directions. We have implemented a customizable Java framework that supports experimentation by simulating a socio-technical system. Results from our experiments suggest effective and precise support for a wide range of self-repairing decisions in a socio-technical setting.
An overview of sleep and circadian dysfunction in Parkinson's disease

Sleep and circadian alterations are amongst the very first symptoms experienced in Parkinson's disease, and sleep alterations are present in the majority of patients with overt clinical manifestation of Parkinson's disease. However, the magnitude of sleep and circadian dysfunction in Parkinson's disease, and its influence on the pathophysiology of Parkinson's disease remains often unclear and a matter of debate. In particular, the confounding influences of dopaminergic therapy on sleep and circadian dysfunction are a major challenge, and need to be more carefully addressed in clinical studies. The scope of this narrative review is to summarise the current knowledge around both sleep and circadian alterations in Parkinson's disease. We provide an overview on the frequency of excessive daytime sleepiness, insomnia, restless legs, obstructive apnea and nocturia in Parkinson's disease, as well as addressing sleep structure, rapid eye movement sleep behaviour disorder and circadian features in Parkinson's disease. Sleep and circadian disorders have been linked to pathological conditions that are often co‐morbid in Parkinson's disease, including cognitive decline, memory impairment and neurodegeneration. Therefore, targeting sleep and circadian alterations could be one of the earliest and most promising opportunities to slow disease progression. We hope that this review will contribute to advance the discussion and inform new research efforts to progress our knowledge in this field.
Salmonella in the laying hen. 1. Salmonella recovery from viscera, feces and eggs following oral inoculation.

Abstract A study was made to determine the fate of Samonella organisms after ingestion by the laying hen. Three species of Salmonella were used, i.e., senftenberg, thompson and typhimurium. Approximately one million cells of each species were introduced into the crop of 12 White Leghorn type laying hens for 10 days. The trials involving each species were carried out consecutively with thorough disinfection of equipment between trials. From each hen, feces and eggs were analyzed daily for Salmonella for the 10 day period. At the end of the 10 day trial a sample of blood was drawn from each hen for Salmonella analysis. In addition, the hens were slaughtered and a sample of the ovaries, kidneys, heart, liver and lungs was aseptically removed for analysis. Results showed that approximately 25% of the fecal samples were found to contain the species of Salmonella under study. The percentage of positive recoveries from egg shells was less than 10% for all three species studied. Among the egg contents examined for all three species, only one egg yielded a positive recovery. All tissue samples were found to be negative. It was concluded that there was no contamination of body tissues even after continuous ingestion of large doses of Salmonella organisms. Neither was there a problem of egg meat contamination among intact eggs.
A quantitative comparison between SOX10 and MART‐1 immunostaining to detect melanocytic hyperplasia in chronically sun‐damaged skin

Histologic differentiation of melanoma in situ (MIS) from solar keratosis on chronically sun‐damaged skin is challenging. The first‐line immunostain is usually MART‐1/Melan‐A, which can exaggerate the epidermal melanocytes, causing a diagnostic pitfall for MIS. By comparing MART‐1 and SOX10 immunostaining, we scored the percentage of epidermal melanocytes per 2‐mm diameter fields in pigmented actinic keratosis (n = 16), lichenoid keratosis (n = 7), junctional melanocytic nevus (n = 6), keratosis with atypical melanocytic proliferation (n = 17) and MIS (n = 10). These cases represented an older population (68 years median age) and the head and neck (50%) was the most common anatomic site. MART‐1 score was significantly higher than SOX10 (P value <.05) in solar keratoses, but showed no difference in detecting melanocytic proliferations, demonstrating their equal detection rate of melanocytes. The sensitivity of both MART‐1 and SOX10 was 100%, while their specificities were 17% and 96%, respectively. These results show that SOX10 is more specific than MART‐1 in distinguishing epidermal melanocytes on sun‐damaged skin by avoiding overdiagnosis of melanoma.
Critères de qualité d'un classifieur généraliste

This paper considers the problem of choosing a good classifier. For each problem there exist an optimal classifier, but none are optimal, regarding the error rate, in all cases. Because there exists a large number of classifiers, a user would rather prefer an all-purpose classifier that is easy to adjust, in the hope that it will do almost as good as the optimal. In this paper we establish a list of criteria that a good generalist classifier should satisfy . We first discuss data analytic, these criteria are presented. Six among the most popular classifiers are selected and scored according to these criteria. Tables allow to easily appreciate the relative values of each. In the end, random forests turn out to be the best classifiers.
Analysis of serum microRNA expression in male workers with occupational noise-induced hearing loss

Occupational noise-induced hearing loss (ONIHL) is a prevalent occupational disorder that impairs auditory function in workers exposed to prolonged noise. However, serum microRNA expression in ONIHL subjects has not yet been studied. We aimed to compare the serum microRNA expression profiles in male workers of ONIHL subjects and controls. MicroRNA microarray analysis revealed that four serum microRNAs were differentially expressed between controls (n=3) and ONIHL subjects (n=3). Among these microRNAs, three were upregulated (hsa-miR-3162-5p, hsa-miR-4484, hsa-miR-1229-5p) and one was downregulated (hsa-miR-4652-3p) in the ONIHL group (fold change >1.5 and Pbon value <0.05). Real time quantitative PCR was conducted for validation of the microRNA expression. Significantly increased serum levels of miR-1229-5p were found in ONIHL subjects compared to controls (n=10 for each group; P<0.05). A total of 659 (27.0%) genes were predicted as the target genes of miR-1229-5p. These genes were involved in various pathways, such as mitogen-activated protein kinase (MAPK) signaling pathway. Overexpression of miR-1229-5p dramatically inhibited the luciferase activity of 3′ UTR segment of MAPK1 (P<0.01). Compared to the negative control, HEK293T cells expressing miR-1229-5p mimics showed a significant decline in mRNA levels of MAPK1 (P<0.05). This preliminary study indicated that serum miR-1229-5p was significantly elevated in ONIHL subjects. Increased miR-1229-5p may participate in the pathogenesis of ONIHL through repressing MAPK1 signaling.
ADMultiImg: a novel missing modality transfer learning based CAD system for diagnosis of MCI due to AD using incomplete multi-modality imaging data

Alzheimer’s Disease (AD) is the most common cause of dementia and currently has no cure. Treatments targeting early stages of AD such as Mild Cognitive Impairment (MCI) may be most effective to deaccelerate AD, thus attracting increasing attention. However, MCI has substantial heterogeneity in that it can be caused by various underlying conditions, not only AD. To detect MCI due to AD, NIA-AA published updated consensus criteria in 2011, in which the use of multi-modality images was highlighted as one of the most promising methods. It is of great interest to develop a CAD system based on automatic, quantitative analysis of multi-modality images and machine learning algorithms to help physicians more adequately diagnose MCI due to AD. The challenge, however, is that multi-modality images are not universally available for many patients due to cost, access, safety, and lack of consent. We developed a novel Missing Modality Transfer Learning (MMTL) algorithm capable of utilizing whatever imaging modalities are available for an MCI patient to diagnose the patient’s likelihood of MCI due to AD. Furthermore, we integrated MMTL with radiomics steps including image processing, feature extraction, and feature screening, and a post-processing for uncertainty quantification (UQ), and developed a CAD system called “ADMultiImg” to assist clinical diagnosis of MCI due to AD using multi-modality images together with patient demographic and genetic information. Tested on ADNI date, our system can generate a diagnosis with high accuracy even for patients with only partially available image modalities (AUC=0.94), and therefore may have broad clinical utility.