Miracle-dz/UniFAR-FTUs huggingface dataset:
Phosphorylation of Protein Tyrosine Residues in Fresh and Cryopreserved Stallion Spermatozoa under Capacitating Conditions1

Abstract Phosphorylation of tyrosine residues on sperm proteins is one important intracellular mechanism regulating sperm function that may be a meaningful indicator of capacitation. There is substantial evidence that cryopreservation promotes the capacitation of sperm and this cryocapacitation is frequently cited as one factor associated with the reduced longevity of cryopreserved sperm in the female reproductive tract. This study was designed to determine whether stallion sperm express different levels of tyrosine phosphorylation after in vitro capacitation and whether thawed sperm display similar phosphorylation characteristics in comparison with freshly ejaculated sperm. Experiments were performed to facilitate comparisons of tyrosine phosphorylation, motility, and viability of sperm prior to and following in vitro capacitation in fresh and frozen-thawed sperm. We hypothesized that equine spermatozoa undergo tyrosine phosphorylation during capacitation and that this phosphorylation is modified when sperm have been cryopreserved. We also hypothesized that tyrosine phosphorylation could be enhanced by the use of the activators dibutyryl cAMP (db cAMP) and caffeine, as well as methyl β-cyclodextrin—which causes cholesterol efflux from the spermatozoa—and inhibited by the protein kinase A (PK-A) inhibitor H-89. Our results indicate that equine sperm capacitation is mediated by a signaling pathway that involves cAMP-dependent PK-A and tyrosine kinases and that cryopreserved sperm may be more sensitive to inducers of capacitation, which could explain their limited life span when compared with fresh sperm.
Scientific integrity and the market for lemons

Scientific integrity cannot be adequately ensured by appeals to the ethical principles of individual researchers. Research fraud has become a public scandal, exacerbated by our inability accurately to judge its extent. Current reliance on peer review of articles ready for publication as the sole means to control the quality and integrity of the majority of research has been shown to be inadequate, partly because faults in the research process may be concealed and partly because anonymous peer review is itself imperfect. Consequently, the scientific literature is mixed, with the reader unable always to distinguish the good articles from the bad. Scientific research is subject to market forces that will always provide a motivation for a range of misdemeanours. This has led to a ‘market for lemons’. Regulations, and sanctions against miscreants, need to be modelled on those historically found necessary to limit financial fraud. Practical and effective systems of process control and audit have already been devised to ensure the integrity of clinical and pre-clinical research. These should be adapted for use in a much wider range of research activities.
Precursor lesions of esophageal cancer in high‐risk populations in henan province, china

This report discusses precancerous changes in the esophageal mucosa from three points of view: the histopathologic features of the esophageal mucosa in persons known to be at high risk for esophageal carcinoma (EC); the histopathologic features of the esophageal mucosa of asymptomatic persons randomly selected from areas at high and low risk for EC; and a prospective follow‐up of a randomly examined group to determine the impact of esophagitis and dysplasia upon subsequent development of EC. Esophagitis was commonly found at endoscopic examination, but there was no difference in frequency of esophagitis in the randomly selected subjects from high‐risk and low‐risk areas. Although one third of patients with dysplasia developed cancer over a follow‐up period of 30 to 78 months, only 4% of those with esophagitis alone developed EC. The authors conclude that dysplasia, diagnosed by cytologic or histologic examination, is a precancerous state, and that esophagitis is a nonspecific pathologic condition of the esophagus, but moderate and severe types of chronic esophagitis might create an environment favorable for the development of esophageal carcinoma.
Correlative Förster Resonance Electron Transfer‐Proximity Ligation Assay (FRET‐PLA) Technique for Studying Interactions Involving Membrane Proteins

This unit provides a guide and detailed protocol for studying membrane protein‐protein interactions (PPI) using the acceptor‐sensitized Förster resonance electron transfer (FRET) method in combination with the proximity ligation assay (PLA). The protocol in this unit is focused on the preparation of FRET‐PLA samples and the detection of correlative FRET/PLA signals as well as on the analysis of FRET‐PLA data and interpretation of correlative results when using cyan fluorescent protein (CFP) as a FRET donor and yellow fluorescent protein (YFP) as a FRET acceptor. The correlative application of FRET and PLA combines two powerful tools for monitoring PPI, yielding results that are more reliable than with either technique alone. © 2016 by John Wiley & Sons, Inc.
Adaptive Algorithm for Blind Separation from Noisy Time-Varying Mixtures

This article addresses the problem of blind source separation from time-varying noisy mixtures using a state variable model and recursive estimation. An estimate of each source signal is produced real time at the arrival of new observed mixture vector. The goal is to perform the separation and attenuate noise simultaneously, as well as to adapt to changes that occur in the mixing system. The observed data are projected along the eigenvectors in signal subspace. The subspace is tracked real time. Source signals are modeled using low-order AR (autoregressive) models, and noise is attenuated by trading off between the model and the information provided by measurements. The type of zero-memory nonlinearity needed in separation is determined on-line. Predictor-corrector filter structures are proposed, and their performance is investigated in simulation using biomedical and communications signals at different noise levels and a time-varying mixing system. In quantitative comparison to other widely used methods, significant improvement in output signal-to-noise ratio is achieved.
Short-term growth hormone treatment does not increase muscle protein synthesis in experienced weight lifters.

The purpose of this study was to determine whether recombinant human growth hormone (GH) administration enhances muscle protein anabolism in experienced weight lifters. The fractional rate of skeletal muscle protein synthesis and the whole body rate of protein breakdown were determined during a constant intravenous infusion of [13C]leucine in 7 young (23 +/- 2 yr; 86.2 +/- 4.6 kg) healthy experienced male weight lifters before and at the end of 14 days of subcutaneous GH administration (40 microgram.kg-1 x day-1). GH administration increased fasting serum insulin-like growth factor-I (from 224 +/- 20 to 589 +/- 80 ng/ml, P = 0.002) but did not increase the fractional rate of muscle protein synthesis (from 0.034 +/- 0.004 to 0.034 +/- 0.002%/h) or reduce the rate of whole body protein breakdown (from 103 +/- 4 to 108 +/- 5 mumol.kg-1 x h-1). These findings suggest that short-term GH treatment does not increase the rate of muscle protein synthesis or reduce the rate of whole body protein breakdown, metabolic alterations that would promote muscle protein anabolism in experienced weight lifters attempting to further increase muscle mass.
TSMC: A Novel Approach for Live Virtual Machine Migration

Cloud computing attracted more and more attention in recent years, and virtualization technology is the key point for deploying infrastructure services in cloud environment. It allows application isolation and facilitates server consolidation, load balancing, fault management, and power saving. Live virtual machine migration can effectively relocate virtual resources and it has become an important management method in clusters and data centers. Existing precopy live migration approach has to iteratively copy redundant memory pages; another postcopy live migration approach would lead to a lot of page faults and application degradation. In this paper, we present a novel approach called TSMC (three-stage memory copy) for live virtual machine migration. In TSMC, memory pages only need to be transmitted twice at most and page fault just occurred in small part of dirty pages. We implement it in Xen and compare it with Xen’s original precopy approach. The experimental results under various memory workloads show that TSMC approach can significantly reduce the cumulative migration time and total pages transferred and achieve better network IO performance in the same time.
Homeward Bound?: Micro-Level Legislative Responsiveness to Ballot Initiatives

Recent macro-level studies examining the indirect effects of direct legislation on public policy in the American states are decidedly mixed. This study tests whether the macro-level logic of legislative behavior in response to ballot initiatives holds true at the micro-level for individual legislators. I examined the determinants of legislative votes on “counter-majoritarian” legislation—bills that directly challenge the outcomes of earlier statewide ballot initiatives. In 1999, the Colorado state legislature tried to overturn the outcomes of three previous ballot contests. I find that in two of the three cases, a legislator's vote on these bills was related to the vote in his or her district on the respective ballot initiative. This helps explain why many legislators will vote contrary to the outcome of a statewide initiative vote.
Relationship Between Body Mass Index and Fracture Risk Is Mediated by Bone Mineral Density

The relationship between body mass index (BMI) and fracture risk is controversial. We sought to investigate the effect of collinearity between BMI and bone mineral density (BMD) on fracture risk, and to estimate the direct and indirect effect of BMI on fracture with BMD being the mediator. The study involved 2199 women and 1351 men aged 60 years or older. BMI was derived from baseline weight and height. Femoral neck BMD was measured by dual‐energy X‐ray absorptiometry (DXA; GE‐LUNAR, Madison, WI, USA). The incidence of fragility fracture was ascertained by X‐ray reports from 1991 through 2012. Causal mediation analysis was used to assess the mediated effect of BMD on the BMI‐fracture relationship. Overall, 774 women (35% of total women) and 258 men (19%) had sustained a fracture. Approximately 21% of women and 20% of men were considered obese (BMI ≥ 30). In univariate analysis, greater BMI was associated with reduced fracture risk in women (hazard ratio [HR] 0.92; 95% confidence interval [CI], 0.85 to 0.99) and in men (HR 0.77; 95% CI, 0.67 to 0.88). After adjusting for femoral neck BMD, higher BMI was associated with greater risk of fracture in women (HR 1.21; 95% CI, 1.11 to 1.31) but not in men (HR 0.96; 95% CI, 0.83 to 1.11). Collinearity had minimal impact on the BMD‐adjusted results (variance inflation factor [VIF] = 1.2 for men and women). However, in mediation analysis, it was found that the majority of BMI effect on fracture risk was mediated by femoral neck BMD. The overall mediated effect estimates were −0.048 (95% CI, −0.059 to −0.036; p < 0.001) in women and −0.030 (95% CI, −0.042 to −0.018; p < 0.001) in men. These analyses suggest that there is no significant direct effect of BMI on fracture, and that the observed association between BMI and fracture risk is mediated by femoral neck BMD in both men and women. © 2014 American Society for Bone and Mineral Research.
Use of personal digital assistants to deliver education about adherence to antiretroviral medications.

National surveys indicate that slightly less than one half of adults in the United States have low or limited literacy skills,1 a hidden characteristic that often hinders effective communication among patients and their health care providers. Literacy is increasingly recognized as an important factor that may influence the course of treatment and several health outcomes.2 Critical to the success of drug therapy is health literacy or the “the degree to which individuals have the capacity to obtain, process, and understand basic health information and services needed to make appropriate health decisions. ”3 The importance of health literacy for the safety and effectiveness of health care is explained in a recent Institute of Medicine report, which includes all health care providers as part of a comprehensive plan to improve health literacy in America.4 Low literacy skills may be particularly problematic for people living with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) because treatment typically involves frequent health care visits and complex medication regimens. Kalichman and Rompa5 found that low health literacy was associated with higher HIV viral loads, lower CD4+ counts, poorer perceived health, and greater numbers of hospitalizations. Furthermore, Kalichman et al.6 observed that people with low health literacy were at increased risk for missing doses of antiretroviral medications. Hence, the relationship between literacy and poor health outcomes in HIV/AIDS patients may be partially explained by how well individuals understand and adhere to prescribed treatment regimens. Although previous studies suggest that medication adherence can be improved through patient education and training,7–9 few literacy-independent tools are available to assist clinicians with medication counseling. Nonetheless, advances in computer technology offer new opportunities for developing innovative strategies that improve communication between health care providers and their patients. Handheld devices such as personal digital assistants (PDAs) may be especially useful in busy ambulatory care settings because they are portable, inexpensive, and enable the use of audio and graphical images to reinforce other forms of communication.
One hit, two hits, three hits, more? Genomic changes in the development of retinoblastoma

The childhood eye cancer retinoblastoma is initiated by the loss of both alleles of the prototypic tumor suppressor gene, RB1. However, a large number of cytogenetic and comparative genomic hybridization (CGH) studies have shown that these M1 and M2 mutational events—although necessary for initiation—are not the only genomic changes in retinoblastoma. Some of these subsequent changes, which we have termed M3 to Mn, are likely crucial for tumor progression not only in retinoblastoma but also in other cancers. Moreover, genes showing genomic change in cancer are more stable markers and, therefore, possible therapeutic targets than genes simply differentially expressed. In this review, we provide the first comprehensive summary of the genomic evidence implicating gain of 1q, 2p, 6p, and 13q, and loss of 16q in retinoblastoma oncogenesis, including karyotype, CGH, and microarray CGH data. We discuss the search for candidate oncogenes and tumor suppressor genes within these regions, including the candidates (KIF14, MDM4, MYCN, E2F3, DEK, CDH11, and others), plus associations between genomic changes and clinical parameters. We also review studies of other regions of the retinoblastoma genome, the epigenetic changes of aberrant methylation of MGMT, RASSF1A, CASP8, and MLH1, and the roles microRNAs might play in this cancer. Although many candidate genes have yet to be functionally validated in retinoblastoma, work in this field lays out a molecular cytogenetic pathway of retinoblastoma development. Candidate cancer genes carry diagnostic, prognostic, and therapeutic implications beyond retinoblastoma. © 2007 Wiley‐Liss, Inc.
School‐based asthma surveillance: A comparison of student and parental report

This study addressed the comparability of data obtained from a student‐based and parent‐based asthma and respiratory health survey. Our goal was to ascertain whether there were meaningful and systematic differences in asthma classification based on symptom and diagnosis reports obtained separately from students and their parents. A brief, written survey, based on the International Study of Asthma and Allergy in Children questionnaire, was administered to 6th through 10th grade students in two schools in Oakland, CA, USA. Students who reported asthma‐like indicators for the previous 12‐month period were defined as positive and a more extensive questionnaire was mailed home to those parents. A more refined classification of asthma based on parent report of indicators was compared with student report. Forty‐four percent of 1298 students were classified as positive for current asthma‐like symptoms and 50% of parent surveys were returned. For the positive students with parent surveys, 59% were classified as ‘probable’ for asthma based on the parent survey. Overall, the agreement between parent and students’ classification was 70%, and 83% for students with a parent report of physician diagnosis of asthma. Students who were discordant with parents for physician diagnosis of asthma were more likely to be male, and more likely to have a parent report of unscheduled Emergency Department visit for wheezing or trouble breathing. Findings indicated that with the exception of medication, students reported asthma indicators more frequently that parents, independent of classification. Student report of physician diagnosis with a 12‐month report of an asthma symptom was determined to be a good indicator of probable current asthma. Inclusion of or reliance on a parental questionnaire is not likely to improve the reliability of a school‐based asthma surveillance program in our population.
100-Gb/s Direct-Detection OFDM Transmission on Independent Polarization Tributaries

We propose a low-overhead method of polarization-multiplexed direct detection optical orthogonal frequency-division-multiplexing transmission that requires no digital multiple-input and multiple-output processing at the receiver. Optical filtering of carrier components allows a simple polarization separation. Using 16-quadrature amplitude modulation and only 31-GHz bandwidth, 100-Gb/s transmission over 80 km of standard single-mode fiber is successfully demonstrated. The crosstalk penalty was only 1.2 dB. We also investigated the laser frequency offset tolerance for the proposed 100-Gb/s transmission method.
The Impact of Type 2 Diabetes on the Development of Hepatocellular Carcinoma in Different Viral Hepatitis Statuses

Background: The risk of type 2 diabetes on the development of hepatocellular carcinoma remains inconclusive in different hepatitis statuses. Methods: We prospectively followed a community-based cohort with 5,929 persons in southern Taiwan from January 1997 through December 2004, made up of 4,117 seronegative, 982 anti–hepatitis C virus–positive [HCV(+)], 696 hepatitis B surface antigen–positive [HBsAg(+)], and 134 coinfected persons. Before the study, 546 participants had developed diabetes. Hepatocellular carcinoma diagnoses were from the National Cancer Registry. Results: After 50,899 person-years of follow-up, 111 individuals had developed hepatocellular carcinoma. The highest risk of hepatocellular carcinoma, compared with seronegative individuals without diabetes, was in anti-HCV(+) individuals with diabetes [incidence rate ratio (IRR), 76.0], then coinfected (IRR, 46.0), anti-HCV(+) without diabetes (IRR, 26.1), HBsAg(+) with diabetes (IRR, 21.4), and seronegative with diabetes (IRR, 7.2; P < 0.001). Anti-HCV(+) (n = 132) and seronegative individuals (n = 352) with diabetes had a higher cumulative incidence rate of hepatocellular carcinoma than those without diabetes (log-rank test, P < 0.001). Multivariate Cox proportional hazards analysis showed that gender, age, body mass index ≥30, HBsAg(+) [hazards ratio (HR), 12.6], anti-HCV(+) (HR, 18.8), coinfection (HR, 25.9), and diabetes [HR, 2.7; 95% confidence interval (95% CI), 1.7-4.3] were independent predictors of hepatocellular carcinoma (P < 0.05). After stratifying hepatitis status in multivariate Cox analysis, diabetes was significant for seronegative (HR, 5.4; 95% CI, 1.7-17.1) and anti-HCV(+) individuals (HR, 3.1; 95% CI, 1.7-5.4). Body mass index ≥30 was significant for HBsAg(+) individuals (HR, 3.3; 95% CI, 1.3-8.1). Conclusion: Type 2 diabetes is a strong independent predictor of hepatocellular carcinoma in anti-HCV(+) and seronegative individuals but not in HBsAg(+) individuals. (Cancer Epidemiol Biomarkers Prev 2009;18(7):2054–60)
On the Multiple-view Triangulation Problem with Perspective and Non-perspective Cameras - A Virtual Reprojection-based Approach

Abstract: This paper considers the multiple-view triangulation problem in a vision system with perspective and nonperspective cameras. In particular, cameras that can be modeled through a spherical projection followed by a perspective one, such as perspective cameras and fisheye cameras, are considered. For this problem, an approach based on reprojecting the available image points onto virtual image planes is proposed, which has the advantage of transforming the original problem into a new one for which the existing methods for multipleview triangulation with perspective cameras can be used. In particular, algebraic and geometric errors of such methods are now evaluated on the virtual image planes, and the solution of the new problem exactly approaches the sought scene point as image noise and calibration errors tend to zero. The proposed approach is illustrated by several numerical investigations with synthetic and real data.
An Improved Foreground Object Detection Method Based on Gaussian Mixture Models

Statistical background subtraction has proved to be a robust and effective approach for segmenting and extracting objects without any prior information of the foreground objects. This paper presents two contributions on this topic. The first contribution of this paper proposes a novel approach which introduces the motion mask into the Gaussian Mixture Models to reduce the errors of classical GMMs, which always classifies the moving objects as background incorrectly, and affects the accuracy of the steps followed by, when the objects are still in long periods. The second contribution regards the connected component labeling based on the contour tracking algorithm. Experimental results validate the effectiveness of the proposed approach.
The Nottingham Fatigue after Stroke (NotFAST) study: factors associated with severity of fatigue in stroke patients without depression

Objective: To identify factors associated with post-stroke fatigue in a sample of stroke survivors without depression. Design: Cross-sectional cohort study. Setting: Recruitment was from four stroke units in the UK. Subjects: Participants were assessed within four to six weeks of first stroke; those with high levels of depressive symptoms (score ⩾7 Brief Assessment Schedule Depression Cards) were excluded. Main measures: Participants were assessed after stroke on the Fatigue Severity Scale of the Fatigue Assessment Inventory, the Rivermead Mobility Index, Nottingham Extended Activities of Daily Living scale, Beck Anxiety Index, Sleep Hygiene Index, 6m walk test, and measures of cognitive ability. Results: Of the 371 participants recruited, 103 were excluded and 268 were assessed. Of the latter, the mean age was 67.7 years (SD 13.5) and 168 (63%) were men. The National Institutes of Health Stroke Scale mean score was 4.96 (SD 4.12). Post-stroke fatigue was reported by 115 (43%) of participants, with 71 (62%) reporting this to be a new symptom since their stroke. Multivariate analysis using the Fatigue Severity Scale as the outcome variable found pre-stroke fatigue, having a spouse/partner, lower Rivermead Mobility Index score, and higher scores on both the Brief Assessment Schedule Depression Cards and Beck Anxiety Index were independently associated with post-stroke fatigue, accounting for approximately 47% of the variance in Fatigue Severity Scale scores. Conclusions: Pre-stroke fatigue, lower mood, and poorer mobility were associated with post-stroke fatigue.