Miracle-dz/UniFAR-FTUs huggingface dataset:
Retinal ischemic preconditioning in the rat: requirement for adenosine and repetitive induction.

PURPOSE A brief period of ischemia can induce a remarkably complete state of ischemic tolerance in the retina, a phenomenon known as ischemic preconditioning (IPC). The mechanisms of IPC were studied in the rat retina by examining the role of adenosine as a possible mediator and determining whether IPC protection could be induced more than once in the same rat. METHODS Retinal ischemia was produced for 60 minutes in ketamine-xylazine-anesthetized Sprague-Dawley rats, and recovery was measured using electroretinography. Twenty-four hours earlier, the IPC stimulus of 5 minutes of ischemia was applied. To test the role of adenosine as a mediator of IPC, the selective adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX; 0.45 mg/kg, 2.25 mg/kg, or 4.5 mg/kg), the A2a antagonist 8-(3-chlorostyryl)caffeine (CSC; 0.1 mg/kg or 1.0 mg/kg), or their cyclodextrin vehicle were administered 15 minutes before IPC. To examine whether exogenous adenosine administration could mimic IPC, animals received intravitreal injections of the adenosine A1 receptor stimulant adenosine amine congener (ADAC) or the A2a stimulant CGS21680, followed by ischemia 24 hours later. To test the hypothesis that IPC could be induced repeatedly without loss of protection, rats were divided to receive IPC or sham IPC, followed 10 days later by IPC or a sham procedure, and 24 hours later by 60 minutes of ischemia. RESULTS Adenosine A1 receptor blockade with 4.5 mg/kg DPCPX administered intraperitoneally (IP) before or immediately after 5 minutes of ischemia completely blocked IPC protection, whereas lower doses resulted in partial blockade. CSC at the lowest dose (0.1 mg/kg) had no significant effect on IPC's protective effect, whereas partial blockade was found with 1.0 mg/kg CSC. A1 or A2a receptor stimulation produced partial but significant mimicking of IPC protection, effects that were antagonized by DPCPX or CSC. Ischemic preconditioning applied twice, separated by 10 days, and followed by 60 minutes of ischemia 24 hours after the second IPC stimulus, resulted in nearly identical recovery of function after ischemia compared with IPC performed one time. CONCLUSIONS Adenosine, acting through the A1 and A2a receptors, is a critical component in the induction of ischemic tolerance after preconditioning in the retina. The neuroprotective effects of IPC in the retina are lost over time but may be reinduced by subsequent application of the IPC stimulus.
Managing Multiple Influences: Self-Monitoring and Social Comparison at the Same Time and Context

Persuasive systems that combine numerous behavior change tech- niques such as self-monitoring and social comparison, expose users to multiple influences. These influences can in turn generate motivation and/or stress. In a previous study with users of three commercial applications from the area of sports, we found that users generated a series of strategies to manage these mul- tiple influences in order to control their negative effects. Moreover, the man- agement took place in different times and contexts, as the techniques were de- signed to be used separately: self-monitoring during exercising, and social comparison before or after the activity. This paper presents a study in progress where we explore how the management of multiple influences takes place under a new condition: when users are exposed at the same time and context to the multiple influences from self-monitoring and social comparison. We will design a prototype that delivers the influences from these two techniques under a sin- gle platform and evaluate it in a laboratory test.
Cost-effectiveness of World Health Organization 2010 Guidelines for Prevention of Mother-to-Child HIV Transmission in Zimbabwe

We projected outcomes for mothers and infants following World Health Organization–recommended regimens to prevent mother-to-child human immunodeficiency virus (HIV) transmission. Compared with Option A, Option B improves life expectancy and saves money; compared with Option B, lifelong maternal therapy is of comparable value to common HIV-related interventions.
Normovolemic Hemodilution and Lumbar Epidural Anesthesia

This randomized study was designed to determine the cardiovascular effects of normovolemic hemodilution and lumbar epidural anesthesia in patients scheduled for vascular surgery. The patients were randomly assigned to three different groups: group 1 (N = 10) included patients undergoing lumbar epidural anesthesia without hemodilution: group 2 (N = 10) consisted of patients with normo-volemic hemodilution without epidural anesthesia: and in group 3 (N = 10) normovolemic hemodilution was produced during lumbar epidural anesthesia. The three groups included several patients with a history of either myocardial infarction or stable mild angina or treated and controlled hypertension. In group 1, the level of epidural anesthesia reached T-9 ± 1. After lumbar epidural anesthesia and 7 mL/kg colloid infusion, pulmonary capillary wedge pressure increased slightly but significantly above baseline, without significant changes either in mean arterial pressure or in cardiac index. In group 2, the same colloid infusion as in group 1 when infused before normovolemic hemodilution increased pulmonary capillary wedge pressure and cardiac index without significant effects on arterial blood pressure. Normovolemic hemodilution using a colloid solution decreased hemoglobin concentration (18%) and increased cardiac index significantly (9%). No significant change in systemic oxygen transport or in total body oxygen consumption was observed, In group 3, with anesthesia to T-9 ± 1, hemodynamic changes were as observed in group 1. After normovolemic hemodilution, hemoglobin concentration decreased significantly (15%), whereas cardiac index increased significantly (15%) without significant changes either in mean arterial pressure or in heart rate. Systemic oxygen transport and total body oxygen consumption did not change significantly. No patient experienced chest pain or electro-cardiographic evidence of myocardial ischemia. These data demonstrate that the effects of normovolemic hemodilution during lumbar epidural anesthesia on hemodynamic function and oxygenation were minimal, well tolerated, and comparable to those seen during hemodilution alone.
Anti-apoptotic proteins and catalase-dependent apoptosis resistance in nickel chloride-transformed human lung epithelial cells

Chronic exposure to nickel compounds is associated with increased incidence of certain types of human cancer, including lung and nasal cancers. Despite intensive investigation, the oncogenic processes remain poorly understood. Apoptosis resistance is a key feature for tumor cells to escape physiological surveillance and acquire growth advantage over normal cells. Although NiCl2 exposure induces transformation of human lung epithelial cells, little information is available with regard to its molecular mechanisms, it is also not clear if the transformed cells are apoptosis resistant and tumorigenic. We explored the apoptosis resistance properties of nickel chloride-transformed human lung epithelial cells and the underlying mechanisms. The results showed that transformed BEAS-2B human lung epithelial cells are resistant to NiCl2-induced apoptosis. They have increased Bcl-2, Bcl-xL and catalase protein levels over the passage matched non-transformed counterparts. The mechanisms of apoptosis resistance are mitochondria-mediated and caspase-dependent. Forced overexpression of Bcl-2, Bcl-xL and catalase proteins reduced NiCl2-induced cell death; siRNA-mediated knockdown of their expression sensitized the cells to nickel-induced apoptosis, suggesting that Bcl-2, Bcl-xl and catalase protein expression plays a critical role in apoptosis resistance. Akt also participates in this process, as its overexpression increases Bcl-xL protein expression levels and attenuates NiCl2-induced apoptosis. Furthermore, transformed cells are tumorigenic in a xenograft model. Together, these results demonstrate that nickel-transformed cells are apoptosis-resistant and tumorigenic. Increased expression of Bcl-2, Bcl-xL and catalase proteins are important mechanisms contributing to transformed cell oncogenic properties.
Nonparametric Item Analyses of the Beck Depression Inventory: Evaluating Gender Item Bias and Response Option Weights

Since the introduction of the Beck Depression Inventory (BDI; Beck, Ward, Mendelson, Mock, & Erbaugh, 1961), studies have documented its effectiveness as a self-report measure of depression (for a review, see Beck, Steer, & Garbin, 1988). These studies report on the effectiveness of the BDI within and between groups but do not examine the psychometric properties of the BDI as a function of the severity of depression within groups. Previous research reporting gender differences on the BDI has neither distinguished group mean differences from differences due to gender item bias (Thissen, Steinberg, & Gerrard, 1986) nor examined how such differences between men and women may vary as a function of depression. Furthermore, despite the large number of studies investigating the BDI, few studies have directly assessed the appropriateness of the weights assigned to response options. In this article, we use techniques based on item response theory to evaluate item performance as a function of the severity of depression as well as to examine gender item bias and the appropriateness of the weights assigned to response options in both depressed outpatient and nonpatient college samples. Items on the BDI consist of groups of graded statements, or options, reflecting different degrees of severity for the symptom domain assessed by that item. For each item there are four op
Synergistic Effect of Caffeine and Glucocorticoids on Expression of Surfactant Protein B (SP-B) mRNA

Administration of glucocorticoids and caffeine is a common therapeutic intervention in the neonatal period, but possible interactions between these substances are still unclear. The present study investigated the effect of caffeine and different glucocorticoids on expression of surfactant protein (SP)-B, crucial for the physiological function of pulmonary surfactant. We measured expression levels of SP-B, various SP-B transcription factors including erythroblastic leukemia viral oncogene homolog 4 (ErbB4) and thyroid transcription factor-1 (TTF-1), as well as the glucocorticoid receptor (GR) after administering different doses of glucocorticoids, caffeine, cAMP, or the phosphodiesterase-4 inhibitor rolipram in the human airway epithelial cell line NCI-H441. Administration of dexamethasone (1 µM) or caffeine (5 mM) stimulated SP-B mRNA expression with a maximal of 38.8±11.1-fold and 5.2±1.4-fold increase, respectively. Synergistic induction was achieved after co-administration of dexamethasone (1 mM) in combination with caffeine (10 mM) (206±59.7-fold increase, p<0.0001) or cAMP (1 mM) (213±111-fold increase, p = 0.0108). SP-B mRNA was synergistically induced also by administration of caffeine with hydrocortisone (87.9±39.0), prednisolone (154±66.8), and betamethasone (123±6.4). Rolipram also induced SP-B mRNA (64.9±21.0-fold increase). We detected a higher expression of ErbB4 and GR mRNA (7.0- and 1.7-fold increase, respectively), whereas TTF-1, Jun B, c-Jun, SP1, SP3, and HNF-3α mRNA expression was predominantly unchanged. In accordance with mRNA data, mature SP-B was induced significantly by dexamethasone with caffeine (13.8±9.0-fold increase, p = 0.0134). We found a synergistic upregulation of SP-B mRNA expression induced by co-administration of various glucocorticoids and caffeine, achieved by accumulation of intracellular cAMP. This effect was mediated by a caffeine-dependent phosphodiesterase inhibition and by upregulation of both ErbB4 and the GR. These results suggested that caffeine is able to induce the expression of SP-transcription factors and affects the signaling pathways of glucocorticoids, amplifying their effects. Co-administration of caffeine and corticosteroids may therefore be of benefit in surfactant homeostasis.
Person Detection in the Restaurant of the Future

Many computer vision techniques dealing with humans require that the location within an image of a person is known. In this research, we investigated into the subject of person detection in a real-world environment: the Restaurant of the Future. We evaluated several methods and selected one: person detection using face detection. In order to apply this we evaluated several known methods for face detection. Then we determined which aspects of the recordings from the Restaurant impacted the performance of the face detectors. All methods suffered strongly from the complex background in the Restaurant and the low quality of the recordings. The angled viewpoint had an impact on several of the methods while the video encoding used affected others. On the worst conditions, the Viola-Jones detector performed best. The analysis of the weaknesses of the face detectors will give future researchers a starting point in improving known or finding new methods.
Negation Without Negation in Probabilistic Logic Programming

Probabilistic logic programs without negation can have cycles (with a preference for false), but cannot represent all conditional distributions. Probabilistic logic programs with negation can represent arbitrary conditional probabilities, but with cycles they create logical inconsistencies. We show how allowing negative noise probabilities allows us to represent arbitrary conditional probabilities without negations. Noise probabilities for non-exclusive rules are difficult to interpret and unintuitive to manipulate; to alleviate this we define "probability-strengths" which provide an intuitive additive algebra for combining rules. For acyclic programs we prove what constraints on the strengths allow for proper distributions on the non-noise variables and allow for all non-extreme distributions to be represented. We show how arbitrary CPDs can be converted into this form in a canonical way. Furthermore, if a joint distribution can be compactly represented by a cyclic program with negations, we show how it can also be compactly represented with negative noise probabilities and no negations. This allows algorithms for exact inference that do not support negations to be applicable to probabilistic logic programs with negations.
Human Siglec-5 Inhibitory Receptor and Immunoglobulin A (IgA) Have Separate Binding Sites in Streptococcal β Protein*

Sialic acid-binding immunoglobulin-like lectins (Siglecs) are receptors believed to be important for regulation of cellular activation and inflammation. Several pathogenic microbes bind specific Siglecs via sialic acid-containing structures at the microbial surface, interactions that may result in modulation of host responses. Recently, it was shown that the group B Streptococcus (GBS) binds to human Siglec-5 (hSiglec-5), an inhibitory receptor expressed on macrophages and neutrophils, via the IgA-binding surface β protein, providing the first example of a protein/protein interaction between a pathogenic microbe and a Siglec. Here we show that the hSiglec-5-binding part of β resides in the N-terminal half of the protein, which also harbors the previously determined IgA-binding region. We constructed bacterial mutants expressing variants of the β protein with non-overlapping deletions in the N-terminal half of the protein. Using these mutants and recombinant β fragments, we showed that the hSiglec-5-binding site is located in the most N-terminal part of β (B6N region; amino acids 1–152) and that the hSiglec-5- and IgA-binding domains in β are completely separate. We showed with BIAcoreTM analysis that tandem variants of the hSiglec-5- and IgA-binding domains bind to their respective ligands with high affinity. Finally, we showed that the B6N region, but not the IgA-binding region of β, triggers recruitment of the tyrosine phosphatase SHP-2 to hSiglec-5 in U937 monocytes. Taken together, we have identified and isolated the first microbial non-sialic acid Siglec-binding region that can be used as a tool in studies of the β/hSiglec-5 interaction.
Examining the Relevance of the Transtheoretical Model of Change Within an Alcohol Dependency Treatment Program

Abstract The transtheoretical model of change describes a continuum of behavior change as a transition through the following stages: precontemplation, contemplation, preparation, action, and maintenance. The transtheoretical model's applicability to an alcohol‐dependent population has been briefly examined among individuals entering an alcohol dependency treatment program and people attending Alcoholics Anonymous meetings. However, there continues to be a limited number of studies applying this model to alcohol treatment. The purpose of the study was to discover if the stages of the transtheoretical model of change are compatible with the phases of a Navy/Marine Corps rehabilitation program for the treatment of alcohol dependence. The results found a distinct association between the transtheoretical model of change and phases of alcohol dependency treatment at one rehabilitation center. The transtheoretical model's practical applications to nursing are discussed.
PFIT and PFRIT: Bioinformatic algorithms for detecting glycosidase function from structure and sequence

The identification of the enzymes involved in the metabolism of simple and complex carbohydrates presents one bioinformatic challenge in the post‐genomic era. Here, we present the PFIT and PFRIT algorithms for identifying those proteins adopting the α/β barrel fold that function as glycosidases. These algorithms are based on the observation that proteins adopting the α/β barrel fold share positions in their tertiary structures having equivalent sets of atomic interactions. These are conserved tertiary interaction positions, which have been implicated in both structure and function. Glycosidases adopting the α/β barrel fold share more conserved tertiary interactions than α/β barrel proteins having other functions. The enrichment pattern of conserved tertiary interactions in the glycosidases is the information that PFIT and PFRIT use to predict whether any given α/β barrel will function as a glycosidase or not. Using as a test set a database of 19 glycosidase and 45 nonglycosidase α/β barrel proteins with low sequence similarity, PFIT and PFRIT can correctly predict glycosidase function for 84% of the proteins known to function as glycosidases. PFIT and PFRIT incorrectly predict glycosidase function for 25% of the nonglycosidases. The program PSI‐BLAST can also correctly identify 84% of the 19 glycosidases, however, it incorrectly predicts glycosidase function for 50% of the nonglycosidases (twofold greater than PFIT and PFRIT). Overall, we demonstrate that the structure‐based PFIT and PFRIT algorithms are both more selective and sensitive for predicting glycosidase function than the sequence‐based PSI‐BLAST algorithm.
Reasoning for Semantic Error Detection in Text

Identifying incorrect content (i.e., semantic error) in text is a difficult task because of the ambiguous nature of written natural language and themany factors that canmake a statement semantically erroneous. Current methods identify semantic errors in a sentence by determining whether it contradicts the domain to which the sentence belongs. However, because thesemethods are constructedon expected logic contradictions, they cannot handle new or unexpected semantic errors. In this paper, we propose a new method for detecting semantic errors that is based on logic reasoning. Our proposed method converts text into logic clauses, which are later analyzed against a domain ontology by an automatic reasoner to determine its consistency. This approach can provide a complete analysis of the text, since it can analyze a single sentence or sets of multiple sentences. When there are multiple sentences to analyze, in order to avoid the high complexity of reasoning over a large set of logic clauses, we propose rules that reduce the set of sentences to analyze, based on the logic relationships between sentences. In our evaluation, we have found that our proposed method can identify a significant percentage of semantic errors and, in the case of multiple sentences, it does so without significant computational cost. We have also found that both the quality of the information extraction output and modeling elements B Dejing Dou dou@cs.uoregon. edu Fernando Gutierrez fernando@cs.uoregon. edu Nisansa de Silva nisansa@cs.uoregon. edu Stephen Fickas fickas@cs.uoregon. edu 1 Department of Computer and Information Science, 1202 University of Oregon, Eugene, OR 97403, USA of the ontology (i.e., property domain and range) affect the capability of detecting errors.
Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

Amorphous silica nanoparticles (SiNPs) are being used in biomedical, pharmaceutical, and many other industrial applications entailing human exposure. However, their potential vascular and systemic pathophysiologic effects are not fully understood. Here, we investigated the acute (24 hours) systemic toxicity of intraperitoneally administered 50 nm and 500 nm SiNPs in mice (0.5 mg/kg). Both sizes of SiNPs induced a platelet proaggregatory effect in pial venules and increased plasma concentration of plasminogen activator inhibitor-1. Elevated plasma levels of von Willebrand factor and fibrinogen and a decrease in the number of circulating platelets were only seen following the administration of 50 nm SiNPs. The direct addition of SiNPs to untreated mouse blood significantly induced in vitro platelet aggregation in a dose-dependent fashion, and these effects were more pronounced with 50 nm SiNPs. Both sizes of SiNPs increased lactate dehydrogenase activity and interleukin 1β concentration. However, tumor necrosis factor α concentration was only increased after the administration of 50 nm SiNPs. Nevertheless, plasma markers of oxidative stress, including 8-isoprostane, thiobarbituric acid reactive substances, catalase, and glutathione S-transferase, were not affected by SiNPs. The in vitro exposure of human umbilical vein endothelial cells to SiNPs showed a reduced cellular viability, and more potency was seen with 50 nm SiNPs. Both sizes of SiNPs caused a decrease in endothelium-dependent relaxation of isolated small mesenteric arteries. We conclude that amorphous SiNPs cause systemic inflammation and coagulation events, and alter vascular reactivity. Overall, the effects observed with 50 nm SiNPs were more pronounced than those with 500 nm SiNPs. These findings provide new insight into the deleterious effect of amorphous SiNPs on vascular homeostasis.