Miracle-dz/UniFAR-FTUs huggingface dataset:
A new model of focal cerebral ischemia in the miniature pig.

OBJECT The purpose of this set of studies is to design a minimally invasive, reproducible stroke model in the gyrencephalic brain. This paper provides information on both surgical technique and methods of quantification of ischemic damage to both gray and white matter in the miniature pig. METHODS Sixteen male miniature pigs were randomly divided into three groups and underwent transcranial surgery involving a frontotemporal approach with orbital rim osteotomy for permanent middle cerebral artery occlusion (MCAO; five animals), permanent internal carotid artery occlusion (ICAO; six animals), and a sham operation (five animals). Histological mapping and magnetic resonance (MR) imaging were used to delineate the areas of ischemic damage. The volumes of infarction measured directly from MR images were 16.2 +/- 1.1, 1.5 +/- 0.5, and 0.0 +/- 0.0 cm3 (mean +/- standard deviation [SD], p < 0.001) in the MCAO, ICAO, and sham-operated groups, respectively. The areas of ischemia identified through histological analysis and MR imaging showed a good correlation (r2 = 0.86, p < 0.0001). Immunohistochemical staining with an amyloid precursor protein (APP) antibody was used to evaluate axonal damage and calculate a total APP score for axonal damage of 44.8 +/- 2.9 in the MCAO, 13.2 +/- 6.6 in the ICAO, and 0.0 +/- 0.0 (mean +/- SD, p < 0.002) in the sham-operated animals. CONCLUSIONS This new model of focal cerebral ischemia induces a reproducible amount of ischemic damage in both gray and white matter, and has significant utility for studies of the pathophysiology of ischemia in the gyrencephalic brain and for assessment of the therapeutic efficacy of drugs prior to the initiation of human clinical trials.
Children who Stutter Exchange Linguistic Accuracy for Processing Speed in Sentence Comprehension

Comprehension of predicates and reflexives was examined in children who stutter (CWS) and children who do not stutter (CWNS) who were between 9 years, 7 months and 10 years, 2 months. Demands on working memory and manual reaction time were also assessed in two experiments that employed a four-choice picture-selection sentence comprehension task. CWS were less accurate than CWNS on the attachment of predicates. For reflexives, there was no between-group difference in accuracy, but there was a difference in speed. The two constructions induced processing at different points on a speed–accuracy continuum with CWS sacrificing accuracy to respond fast with predicates, while they maintained accuracy of reflexives by responding slower relative to CWNS. Predicates made more demands on language than nonspeech motor reaction time, whereas the reverse was the case with reflexives for CWS compared to CWNS.
Aromatic Amino Acids in the Juxtamembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Are Important for Receptor-Dependent Virus Entry and Cell-Cell Fusion

ABSTRACT The severe acute respiratory syndrome coronavirus (SARS-CoV) spike glycoprotein (S) is a class I viral fusion protein that binds to its receptor glycoprotein, human angiotensin converting enzyme 2 (hACE2), and mediates virus entry and cell-cell fusion. The juxtamembrane domain (JMD) of S is an aromatic amino acid-rich region proximal to the transmembrane domain that is highly conserved in all coronaviruses. Alanine substitutions for one or two of the six aromatic residues in the JMD did not alter the surface expression of the SARS-CoV S proteins with a deletion of the C-terminal 19 amino acids (S Δ19) or reduce binding to soluble human ACE2 (hACE2). However, hACE2-dependent entry of trypsin-treated retrovirus pseudotyped viruses expressing JMD mutant S Δ19 proteins was greatly reduced. Single alanine substitutions for aromatic residues reduced entry to 10 to 60% of the wild-type level. The greatest reduction was caused by residues nearest the transmembrane domain. Four double alanine substitutions reduced entry to 5 to 10% of the wild-type level. Rapid hACE2-dependent S-mediated cell-cell fusion was reduced to 60 to 70% of the wild-type level for all single alanine substitutions and the Y1188A/Y1191A protein. S Δ19 proteins with other double alanine substitutions reduced cell-cell fusion further, from 40% to less than 20% of wild-type levels. The aromatic amino acids in the JMD of the SARS-CoV S glycoprotein play critical roles in receptor-dependent virus-cell and cell-cell fusion. Because the JMD is so highly conserved in all coronavirus S proteins, it is a potential target for development of drugs that may inhibit virus entry and/or cell-cell fusion mediated by S proteins of all coronaviruses.
Are Indian rice exporters able to price discriminate? Empirical evidence for basmati and non-basmati rice

ABSTRACT The role of exchange rate fluctuations on the pricing behaviour of Indian rice exporters in their major destination markets is examined using the pricing-to-market (PTM) model. The analysis was undertaken in a context where India has emerged as a leading exporter of rice in the world market. The study distinguishes between basmati and non-basmati rice in the analysis as the destination markets differ across these two varieties. One of the key contributions of this study is that it undertakes an analysis under 3 exchange rate models, they are: nominal, real and commodity-specific exchange rates. The results from our analysis indicated the presence of non-competitive pricing behaviour of India’s rice exporters in majority of destination markets due to both the market-specific characteristics as well as exchange rate-induced effects. The amplification of exchange rate effects was more prominent in commodity-specific exchange rate model whereas local currency stabilization was more prominent under nominal and real exchange rate models. Furthermore, the analysis showed that the commodity-specific exchange rate better predicts the PTM behaviour of rice exporters.
Toxicological assessment of drugs that affect the endocrine system in puberty-related disorders

Introduction: Toxicologists must ensure that clinical risk-to-benefit analysis should be made both for genders and age groups, with any treatment. Puberty concerns physiological changes leading to organism's maturation. Pubertal growth disorders are increasing in last decades: besides causing physical and psychological distress, they may signal underlying endocrine-metabolic abnormalities with serious health consequences later on. Therapeutic approaches for some health conditions in childhood and adolescence are considered. Areas covered: The authors discuss how some diseases and treatments can impact pubertal growth. The authors look at particular immunological disorders such as asthma and how both the disease and treatment affects pubertal growth. They also discuss how the provision of available data can help to assess the dose–response of the drug, in these cases, and minimize the chance of side effects. The authors also discuss pediatric inflammatory bowel disease and how both the disease and treatment can mitigate the growth delay. Last, but not least, the authors discuss how the effects of the drugs used in the treatment of psychiatric disorders may accentuate endocrine issues in juvenile patients. Hyperprolactinemia induction by some antipsychotics is highlighted as an example. Expert opinion: Appropriate risk–benefit analysis of drugs prescribed during childhood and adolescence and intended to be used in the long term is required. Furthermore, future treatment strategies and safer compounds development should be supported by the knowledge of mechanisms underlying adverse side effects in pubertal growth and development.
Optimization of Meander Line Antenna Considering Coupling Between Nonlinear Circuit and Electromagnetic Waves for UHF-Band RFID

This paper presents optimization of meander line antennas (MLA) for passive IC tags which contain nonlinear circuits. In the present analysis, the tag antenna loaded by the Cockcroft-Walton circuit is analyzed by solving coupling equations for electromagnetic waves and nonlinear circuit with FDTD method. The antenna shape is optimized so that the output voltage of the Cockcroft-Walton circuit is maximized using the micro genetic algorithm (μ GA) . The computational burden in the optimization processes is effectively suppressed by truncating the transient computations. A criterion is introduced to evaluate the errors caused by the truncation. The present method yields the optimal MLA which has the greater output voltage in comparison with the dipole antenna.
How well does journal 'impact' work in the assessment of papers on clinical physiology and nuclear medicine?

This study reports a citation analysis of 217 scientific papers on clinical physiology and nuclear medicine published in 69 different journals during the years 1985-92. The actual citation frequency was compared with the journal 'impact factor' (i.e. the average number of times a paper is cited in a particular journal in the year of publication and the subsequent year). The average impact factor per paper was 1.96 and per journal 1.92 (range 0.0-22.8). A direct relation was found between the journal impact factor and the citation factor (i.e. the actual citations in the year of publication and the subsequent year) (journals with five or more papers R = 0.69, P < 0.01; all journals R = 0.40, P < 0.001). However, the citation factor was significantly below the journal impact factor (P < 0.0001). An almost linear relation was found between cumulated citations and time (R = 0.99, P < 0.02.-0.0001), but an initial phase of no citation was identified, and the number of citations per year reached a maximum or plateau 3-7 years after publication, which was later than the general maximum of 2-3 years for all medical specialities. In a sample of 200 known autocitations, 75% of the papers had full recovery, 3% mixed recovery and 22% no recovery, giving an overall recovery of 70%. In conclusion, although a phase of no citation was identified, which gave a lower citation factor than the journal impact factor, the citation frequency increased over time, and altogether there seems to be a good agreement between journal impact factor and overall citation frequency of papers on clinical physiology and nuclear medicine.
Multilineage glycosylphosphatidylinositol anchor‐deficient haematopoiesis in untreated aplastic anaemia

Aplastic anaemia and paroxysmal nocturnal haemoglobinuria (PNH) are closely related disorders. In PNH, haematopoietic stem cells that harbour PIGA mutations give rise to blood elements that are unable to synthesize glycosylphosphatidylinositol (GPI) anchors. Because the GPI anchor is the receptor for the channel‐forming protein aerolysin, PNH cells do not bind the toxin and are unaffected by concentrations that lyse normal cells. Exploiting these biological differences, we have developed two novel aerolysin‐based assays to detect small populations of PNH cells. CD59 populations as small as 0·004% of total red cells could be detected when cells were pretreated with aerolysin to enrich the PNH population. All PNH patients displayed CD59‐deficient erythrocytes, but no myelodysplastic syndrome (MDS) patient or control had detectable PNH cells before or after enrichment in aerolysin. Only one aplastic anaemia patient had detectable PNH red cells before exposure to aerolysin. However, 14 (61%) had detectable PNH cells after enrichment in aerolysin. The inactive fluorescent proaerolysin variant (FLAER) that binds the GPI anchors of a number of proteins on normal cells was used to detect a global GPI anchor deficit on granulocytes. Flow cytometry with FLAER showed that 12 out of 18 (67%) aplastic anaemia patients had FLAER‐negative granulocytes, but none of the MDS patients or normal control subjects had GPI anchor‐deficient cells. These studies demonstrate that aerolysin‐based assays can reveal previously undetectable multilineage PNH cells in patients with untreated aplastic anaemia. Thus, clonality appears to be an early feature of aplastic anaemia.
Flutamide has no effect on adrenal androgen response to acute ACTH stimulation in patients with prostatic cancer

Basal levels and adrenocorticotropic hormone (ACTH)‐induced increments (Δ‐values) of serum cortisol, dehydroepiandrosterone (DHA), dehydroepiandrosterone sulfate (DHAS), 4‐androstene‐3,17‐dione (A4), and 17‐hydroxyprogesterone (170HP); basal testosterone (T), luteinizing hormone (LH), serum ASAT, gamma‐GT, and albumin were measured in prostatic cancer patients before and after 6 months of treatment with LH‐RH‐agonist, with flutamide, and with LH‐RH‐agonist + flutamide, respectively.
THE ORGANIZATION STRUCTURE AND THEIR REGIONAL EFFECTS OF NETWORKS IN THE CLUSTERS IN LESS DEVELOPED RURAL AREAS

The relationship between industial clusters and regional development and the network structure of the industial clusters have been pay more attention to in recent years,but,little work has been done on what relationships more important to the network behavior of enterprise and what kind of network structure in the clusters in undeveloped rural areas benefit to regional development. By contrasting the network structure and characteristics of the spin-off networks,the emotion networks,advice networks and cooperation networks in three clusters in Henan Province,this paper uses the social network analysis(SNA) to distinguish the core relationship in the process of enterprise networking in the rural clusters. It also presents that the different network structures in the three clusters have different influences on network learning,trust fostering,collaboration among enterprises and the regional development.
The effects of traumatic brain injury on the predicted and actual performance of a test of prospective remembering

Primary objective: The aim of the present study was to assess prospective memory (PM) with a video-based task using naturalistic stimuli, in a group with long-term disabilities resulting from traumatic brain injury (TBI). The specific focus of the research was on comparing the accuracy of the TBI and control groups’ estimates of successful completion of the prospective memory tasks. Research design: A group of 25 persons with TBI and 20 matched controls were compared on the video-based test of prospective remembering, on a test of retrospective memory (the Logical Memory sub-test of the Wechsler Memory Scale) and tests of executive dysfunction. Correlations between the neuropsychological and PM test scores were calculated and an item analysis of the PM test was undertaken. Procedures: For the PM test, participants were given a scenario involving a robbery, and a list of tasks to be completed in a nearby city centre. The instructions were given twice and on the second occasion they were asked to rate the likelihood of remembering each item. They then watched a videotaped segment showing the perspective of a person first driving and then walking through an unfamiliar city. Their task was to recall each instruction when a relevant cue appeared. Outcomes and results: The TBI group ratings of likelihood of recalling items were entirely equivalent to those made by the controls. On the PM test, however, their performance was significantly reduced. The performance of the TBI group on the PM test was correlated with scores on the Logical Memory and verbal fluency measures. Conclusions: The results showed that, although the TBI group performed more poorly on the PM task, their expectations about how much they would remember were comparable to those of the controls. The findings suggest that persons with TBI may have unrealistic expectations about how much they will remember in a novel situation. Awareness and acceptance of cognitive changes after TBI can be a significant issue for rehabilitation.
Osteopontin: an emerging therapeutic target for anticancer therapy

Distant migration of malignant cells or metastasis is considered one of the hallmarks of tumour progression and makes cancer a most deadly disease. The elevated expression of osteopontin (OPN), a metastasis-associated small integrin-binding ligand N-linked glycoprotein family member has been observed in several cancers and, thus, this protein is considered as a potent prognostic marker during tumour progression. OPN regulates a series of signalling cascades and augments the expression of several oncogenic molecules. Therefore, understanding the molecular mechanism and the signalling pathways by which OPN promotes tumorigenesis may be helpful in designing a novel anticancer therapy. At present, the role of OPN in regulating cancer progression is the subject of intense investigation and targeting OPN might be an appropriate therapeutic strategy for the treatment of cancer. This review is focused on OPN-based anticancer therapy, which may provide a new dimension for the successful treatment of cancer.
Pulmonary edema in patients after liver transplantation

The aim of this study is to determine the incidence of radiological pulmonary edema in elective liver transplant recipients and its relationship to perioperative factors and postoperative course. We reviewed 102 chest radiographs from 34 patients who had undergone orthotopic liver transplantation (OLT). Films were assessed by 2 trained radiologists for evidence of pulmonary edema using a standardized system. Clinical and outcome data from the 34 patients were also recorded. There was a high incidence (47%) of postoperative radiological pulmonary edema that was associated with deterioration in gaseous exchange, elevated pulmonary artery pressure, and increased duration of ventilator dependence and intensive care stay. Eighteen percent of the patients developed edema immediately after surgery, which was associated with greater pulmonary artery pressure and transfusion requirements during surgery. An additional 29% developed edema during the next 16 to 20 hours, but there was no association with fluid replacement. We conclude that pulmonary edema is common after OLT and will influence postoperative recovery in a substantial proportion of transplant recipients. Excess perioperative fluid replacement is unlikely to be the sole mechanism of edema in these patients.
Maximizing Network Lifetime with Energy Efficient Routing Protocol for Wireless Sensor Networks

In the research field of Wireless Sensor Networks, how to reduce the energy consumption of WSN so that the lifetime of WSN can be prolonged is one of the hottest spots. Wireless sensor networks (WSN) lifetime is either superficial or impractical, which prevents us from thoroughly understanding the efficiency of these proposed routing protocols. Routing protocols have significant impact on the overall energy consumption of sensor networks. This project present variety of maximizing system lifetime wireless sensor networks with different energy consumption. Energy consumption for three routing protocols had been analyzed which are direct communication, minimum transmission energy and low adaptive energy clustering hierarchy. The energy consumption for Low Energy Adaptive Clustering Hierarchy (LEACH) routing protocol give the maximum lifetime compared to the others. Clustering is an energy efficient and scalable way to organize the WSN. The main objective of this research is to maximize the lifetime of the wireless sensor (WSN). Simulation via Matlab shows that by applying energy consumption for LEACH routing protocol had increase the network lifetime by as much as 65.2% compared to DC and MTE.
Dynamics analysis and decoupling control for a differential cable drive inertially stabilized platform

In this article, a decoupling control method is proposed to solve the coupling problem of the differential cable drive inertially stabilized platform. First, the transmission chain and motion of the inertially stabilized platform are briefly illustrated, and the dynamics is analysed based on the Lagrange equation. According to the established dynamic equation, the coupling of inertia, torque and angular position between two motors are analysed. Then, the decoupling controller is used to address the coupling problems, and the numerical simulation is realized in the MATLAB/Simulink. The experimental results show that the dynamic equation can accurately describe the dynamic characteristic of the differential cable drive inertially stabilized platform, and the control performance is improved by suppressing the coupling factors.
Autophagy: cellular and molecular mechanisms

Autophagy is a self‐degradative process that is important for balancing sources of energy at critical times in development and in response to nutrient stress. Autophagy also plays a housekeeping role in removing misfolded or aggregated proteins, clearing damaged organelles, such as mitochondria, endoplasmic reticulum and peroxisomes, as well as eliminating intracellular pathogens. Thus, autophagy is generally thought of as a survival mechanism, although its deregulation has been linked to non‐apoptotic cell death. Autophagy can be either non‐selective or selective in the removal of specific organelles, ribosomes and protein aggregates, although the mechanisms regulating aspects of selective autophagy are not fully worked out. In addition to elimination of intracellular aggregates and damaged organelles, autophagy promotes cellular senescence and cell surface antigen presentation, protects against genome instability and prevents necrosis, giving it a key role in preventing diseases such as cancer, neurodegeneration, cardiomyopathy, diabetes, liver disease, autoimmune diseases and infections. This review summarizes the most up‐to‐date findings on how autophagy is executed and regulated at the molecular level and how its disruption can lead to disease. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Primary proteolysis of white brined goat cheese monitored by high molarity Tris buffer SDS- PAGE system

Summary The aim of this work was to investigate primary proteolysis of white brined goat cheese prepared from raw milk and to correlate with the results obtained with high-molarity Tris buffer electrophoretic system. Proteolytic changes of white brined cheese were monitored by three parameters, the total protein, the water soluble proteins and the degree of proteolysis, whereas the change of major casein fractions was followed by electrophoresis in reducing conditions. Ripening caused a decrease of the total protein content, whereas in the first forty days the water soluble protein and the degree of proteolysis increased. Both casein fractions (α s - and β-casein) of goat cheese were susceptible to primary proteolysis but to the different extent. α s1 - casein disappeared during processing, whereas during 60-days of ripening the content of α s2 and β-casein was reduced by 38.90 %, and 30.72 %, respectively. Such trends of major casein fractions were strongly correlated with the degree of proteolysis and the moisture content, whereas no correlation was found between them and the water soluble protein content. The results of our investigation clearly suggested that SDS-PAGE method based on high-molarity Tris-buffer system could be a very useful for purposes of monitoring the white goat cheese proteolysis.