Miracle-dz/UniFAR-FTUs huggingface dataset:
G-Protein Dependent Signal Transduction and Ubiquitination in Dictyostelium

Signal transduction through G-protein-coupled receptors (GPCRs) is central for the regulation of virtually all cellular functions, and it has been widely implicated in human diseases. These receptors activate a common molecular switch that is represented by the heterotrimeric G-protein generating a number of second messengers (cAMP, cGMP, DAG, IP3, Ca2+ etc.), leading to a plethora of diverse cellular responses. Spatiotemporal regulation of signals generated by a given GPCR is crucial for proper signalling and is accomplished by a series of biochemical modifications. Over the past few years, it has become evident that many signalling proteins also undergo ubiquitination, a posttranslational modification that typically leads to protein degradation, but also mediates processes such as protein-protein interaction and protein subcellular localization. The social amoeba Dictyostelium discoideum has proven to be an excellent model to investigate signal transduction triggered by GPCR activation, as cAMP signalling via GPCR is a major regulator of chemotaxis, cell differentiation, and multicellular morphogenesis. Ubiquitin ligases have been recently involved in these processes. In the present review, we will summarize the most significant pathways activated upon GPCRs stimulation and discuss the role played by ubiquitination in Dictyostelium cells.
Symposium on colonic function. Electrophysiology of the colon.

One approach to an analysis of the electrophysiology of smooth muscle and nerves of the large bowel is to examine small bits of them as examples of smooth muscle tissues, without any consideration of the functions of the whole organ. Another approach, more relevant to clinical problems, involves analysis of how the components of the organ are controlled and integrated to form a functional unit. The large bowel transfers the semiliquid contents it receives from the ileum to the rectum in such a way as to allow for extraction of electrolytes and water and for other changes in composition en route. Since evacuation of contents from the rectum is usually a periodic phenomenon, and input by evacuation from the ileum is probably also periodic, there must be coupling in some way between input and output; and there must also be a system of controls to provide for orderly delays and movements of the colonic contents during transit. To understand the control system for transit in the large bowel, a systematic approach seems warranted. I believe one should begin with an analysis of the range of responses which its muscle components can execute (Daniel, 1973). This requires consideration of the origin and integration of spontaneous electrical and mechanical activity of the musculature of the large bowel during normal and abnormal motor activity. A question soon arises: Which responses are initiated and integrated by the smooth muscle alone? Answering this question leads to characterization of the properties of the myogenic control system. If complete, this characterization would provide information about the cellular electrical activities, cell-to-cell coupling in muscle layers and coupling between layers, and the translation of these activities into mechanical responses. A number of gastrointestinal myogenic control systems, particularly of stomach and small bowel (see Daniel, 1973; Sarna, Daniel, and Kingma, 1971, 1972a, b, and c), have been characterized as myogenic systems of mutually coupled relaxation
HUBUNGAN ANTARA PERSEPSI GAYA KEPEMIMPINAN RESONAN DENGAN BURNOUT PADA ANGGOTA KEPOLISIAN BRIGADIR MOBIL (BRIMOB) DETASEMEN A PELOPOR SUBDEN 2 SEMARANG

Instansi Kepolisian selalu melakukan pergantian pemimpin yang berlangsung setiap periode kepemimpinan. Setiap calon pemimpin memiliki gaya dalam memimpin masing-masing. Salah satu tipe gaya kepemimpinan yang diterapkan adalah gaya kepemimpinan resonan dimana pemimpin mampu menggerakan orang-orang dikelompoknya dengan penuh gairah, kekuatan, ketegasan, dan empati. Persepsi negatif anggota akan gaya kepemimpinan resonan menjadikan stresor di lingkungan kerja yang menjurus terjadinya burnout. Penelitian ini bertujuan untuk mengetahui hubungan antara persepsi gaya kepemimpinan resonan dengan burnout pada anggota Kepolisian Brigadir Mobil (Brimob) Detasemen A Pelopor Subden 2 Semarang. Subyek penelitian ini berjumlah 136 anggota. Teknik pengambilan sampel menggunakan convenience sampling. Alat ukur yang digunakan pada penelitian ini adalah Skala Persepsi Gaya Kepemimpinan Resonan (32 aitem, α = 0,943) dan Skala Burnout (28 aitem, α = 0,919) yang diujikan kepada 100 anggota. Analisis Spearman Rho menunjukan nilai rxy= -0,715 dengan p = 0,000 (p < 0,05). Hasil ini menunjukan bahwa adanya hubungan negatif yang signifikan antara persepsi gaya kepemimpinan resonan dengan burnout. Artinya semakin positif persepsi gaya kepemimpinan resonan di instansi tersebut maka akan semakin rendah burnout yang dialami anggota kepolisian.
A cooperative approach for combining client-based and library-based API usage pattern mining

Software developers need to cope with the complexity of Application Programming Interfaces (APIs) of external libraries or frameworks. Typical APIs provide thousands of methods to their client programs, and these methods are not used independently of each other. Much existing work has provided different techniques to mine API usage patterns based on client programs in order to help developers understanding and using existing libraries. Other techniques propose to overcome the strong constraint of clients' dependency and infer API usage patterns only using the library source code. In this paper, we propose a cooperative usage pattern mining technique (COUPminer) that combines client-based and library-based usage pattern mining. We evaluated our technique through four APIs and the obtained results show that the cooperative approach allows taking advantage at the same time from the precision of client-based technique and from the generalizability of library-based techniques.
Financial Valuation and Risk Management Working Paper No . 808 Predictability Hidden by Anomalous Observations

Predictive relations motivated by financial theory should hold at least for the majority of the data, but they can be violated by a small fraction of the data. Conventional testing approaches can fail to detect predictive relations that hold for the vast majority of the data, because rare anomalous observations can have an excessive influence on the results. We propose a novel robust method for testing the null of no predictability, using predictive regression models that might be violated by a minority of anomalous observations. The Monte Carlo evidence demonstrates large improvements of our approach, while the empirical analysis produces a sharp evidence of market return predictability – using predictive variables like the dividend yield, the volatility risk premium, or labor income – which is valid for the vast majority of the data.
even challenges in modeling vaccine preventable diseases

Vaccination has been one of the most successful public health measures since the introduction of basic sanitation. Substantial mortality and morbidity reductions have been achieved via vaccination against many infections, and the list of diseases that are potentially controllable by vaccines is growing steadily. We introduce key challenges for modeling in shaping our understanding and guiding policy decisions related to vaccine preventable diseases.
Monte Carlo Hypothesis Testing with The Sharpe Ratio

Lappeenranta University of technology Department of Technomathematics Senghor Nkuliza Monte Carlo Hypothesis Testing with The Sharpe Ratio Master’s Thesis 2012 71 pages, 42 figures, 7 tables, 2 appendices. Examiners: Prof. PhD. Heikki Haario and Prof. PhD. Eero Patari. Supervisor: Prof. PhD. Heikki Haario.
Finance-Led Capitalism: Shadow Banking, Re-Regulation, and the Future of Global Markets

1. From Sub-Primes to Global Meltdown 2. Long Waves, Structural Crises, and Credit-Money 3. The Foundations of Finance-Led Capitalism 4. Financialization Revisited - A Meso-Economic Approach 5. Shadow Banking as Network Finance 6. The International Monetary System in Flux 7. Re-Regulation Challenges 8. The New Face of Finance
Fungal Growth, Production, and Sporulation during Leaf Decomposition in Two Streams

ABSTRACT I examined the activity of fungi associated with yellow poplar (Liriodendron tulipifera) and white oak (Quercus alba) leaves in two streams that differed in pH and alkalinity (a hardwater stream [pH 8.0] and a softwater stream [pH 6.7]) and contained low concentrations of dissolved nitrogen (<35 μg liter−1) and phosphorus (<3 μg liter−1). The leaves of each species decomposed faster in the hardwater stream (decomposition rates, 0.010 and 0.007 day−1 for yellow poplar and oak, respectively) than in the softwater stream (decomposition rates, 0.005 and 0.004 day−1 for yellow poplar and oak, respectively). However, within each stream, the rates of decomposition of the leaves of the two species were not significantly different. During the decomposition of leaves, the fungal biomasses determined from ergosterol concentrations, the production rates determined from rates of incorporation of [14C]acetate into ergosterol, and the sporulation rates associated with leaves were dynamic, typically increasing to maxima and then declining. The maximum rates of fungal production and sporulation associated with yellow poplar leaves were greater than the corresponding rates associated with white oak leaves in the hardwater stream but not in the softwater stream. The maximum rates of fungal production associated with the leaves of the two species were higher in the hardwater stream (5.8 mg g−1 day−1 on yellow poplar leaves and 3.1 mg g−1 day−1 on oak leaves) than in the softwater stream (1.6 mg g−1day−1 on yellow poplar leaves and 0.9 mg g−1day−1 on oak leaves), suggesting that effects of water chemistry other than the N and P concentrations, such as pH or alkalinity, may be important in regulating fungal activity in streams. In contrast, the amount of fungal biomass (as determined from ergosterol concentrations) on yellow poplar leaves was greater in the softwater stream (12.8% of detrital mass) than in the hardwater stream (9.6% of detrital mass). This appeared to be due to the decreased amount of fungal biomass that was converted to conidia and released from the leaf detritus in the softwater stream.
Surfactant protein expression is increased in the ipsilateral but not contralateral lungs of fetal sheep with left‐sided diaphragmatic hernia

Congenital diaphragmatic hernia (CDH) impairs fetal lung growth and increases the density of alveolar epithelial type 2 (AE2) cells. There is controversy whether surfactant protein (SP) expression is altered in CDH. The primary aim of this study was to assess SP expression (mRNA and protein) in the left and right lungs of fetal sheep with and without a diaphragmatic hernia (DH). Left‐sided DH was created in four fetal sheep at 65 days of gestational age (g.a.). Sham‐operated animals were used as controls. At 138 days g.a. , lungs were harvested and the following parameters were measured: SP‐A, ‐B, and ‐C mRNA expression (Northern blot), SP‐A and ‐B expression (Western blot), and AE2 cell density (immunohistochemistry). The lung weight‐to‐body weight ratio was reduced by 42% in DH animals. The left‐to‐right lung weight ratio was lower in DH animals (0.47 ± 0.03 vs. 0.69 ± 0.03), indicative of asymmetric lung growth. SP‐A, ‐B, and ‐C mRNA expression were increased by 61.7%, 32.9%, and 75.5%, respectively, in the left lungs of DH animals. SP‐A and SP‐B were also increased in DH. In the right lung, SP expression (mRNA and protein) was not different between groups. AE2 cell density was higher (by 67%) in the left but not right lungs of DH animals. Although DH in fetal sheep results in significant lung hypoplasia, SP expression is not reduced. On the contrary, SP expression was increased in the ipsilateral lung of fetuses with left‐sided DH. Furthermore, AE2 cell density is increased in DH, suggesting that the increase in SP mRNA and protein levels is due to increases AE2 cell number. Our data further support the premise that fetal lung hypoplasia favors an AE2 phenotype. Pediatr Pulmonol. 2005; 39:359–367. © 2005 Wiley‐Liss, Inc.
How Do Children Who Can't Hear Learn to Read an Alphabetic Script? A Review of the Literature on Reading and Deafness.

I review the literature on reading and deafness, focusing on the role of three broad factors in acquisition and skilled reading: the method of encoding print; language-specific knowledge (i.e., English); and general language knowledge. I explore the contribution of three communication systems to reading: spoken language, English-based sign, and American Sign Language. Their potential contribution to literacy is mediated by four parameters on which they differ: codability, structural isomorphism, accessibility, and processibility. Finally, I discuss the implications for additional research as well as for education.
Analysis of Supervised and Unsupervised Learning Classifiers for Online Sentiment Analysis

Sentiment analysis is also known as opinion mining which it extracts opinions to learn about public point of view. In general, people prefer to take advice from others not only to get sensible products but also to invest in a wise way. Nowadays, the popular sources of personal opinion are blogs. The consumer review sites are helpful to know about the features and services available to a specific product. This sentiment analysis is also helpful to the manufactures to improve their business by knowing the public opinion. The online sources gather public opinion content in the form of blogs, forums, social media, review websites, etc. The sentiment analysis automatically mines the huge content that is available online. This paper gives a complete study of various supervised and unsupervised classifiers.
Type 2 diabetes and the risk of non-Hodgkin’s lymphoma: a report from two population-based cohort studies in China

Coinciding with the increased incidence of non-Hodgkin’s lymphoma (NHL) during the past decades, there has been a significant increase in the prevalence of diabetes mellitus in mainland China. We therefore evaluated whether type 2 diabetes (T2D) is associated with the risk of NHL using data from the Shanghai Men’s Health Study (SMHS) and the Shanghai Women’s Health Study (SWHS). The SMHS and SWHS are two on-going, prospective, population-based cohorts of more than 130 000 Chinese adults in urban Shanghai. Self-reported diabetes was recorded on the baseline questionnaire and updated in follow-up surveys. Cox regression models with T2D as a time-varying exposure were used to estimate hazard ratios and 95% confidence intervals, adjusting for covariates. After a median follow-up of 12.9 years for SWHS and 7.4 years for SMHS, 172 NHL cases were identified. Patients with T2D have a higher risk of incident NHL with a hazard ratio of 2.00 (95% confidence interval: 1.32–3.03) compared with those without diabetes. This positive association remained when the analysis was restricted to untreated diabetes or after excluding NHL cases that occurred within 3 years after the onset of diabetes. No interaction effect was found in the development of NHL between T2D and other potential risk factors. A linear inverse association was found between T2D duration and the risk of NHL in both men and women (Pfor linearity<0.01), with a highest risk of incident NHL in the first 5 years after the diagnosis of diabetes. Our study suggested that T2D might be associated with an increased risk of NHL.
The Political Economy of Program Design

When programs are grants to states, federal funds will be used to meet both the national objectives and the local priorities of the state or local government recipients. This article examines the decision to design new federal programs as either a grant to states or as administered by federal agencies. We predict that Congress will choose either the states or the federal bureaucracy based on which agent is more likely to manage the program consistent with the preferences of the Congressional majority. We examine the political and economic conditions present in the year before Congress created a program. We find that Congress’s perception of a government agency’s partisan orientation matters: A perceived divergence in partisan orientation between the Congress and federal agency increases the likelihood of a grant design. In addition, we see evidence that a grant design is preferred when the president is not a co-partisan of Congress.
AN EMPIRICAL STUDY OF THE RELATIONSHIPS BETWEEN STRATEGIC MANUFACTURING CAPABILITIES

Dalam kacamata manajemen operasi, kapabilitas strategik mendukung dan membentuk strategi korporat, dan, pada gilirannya, akan membantu kesuksesan perusahaan dalam persaingan. Tujuan dari artikel berikut adalah untuk menguji hubungan antara kapabilitas pemanufakturan strategik pada perusahaan-perusahaan manufaktur di Indonesia. Survei dilakukan menggunakan kuesioner berbasis internet dan uji statistik, dalam hal ini Structural Equation Modeling (SEM), digunakan untuk memahami konsep ini. Analisis terhadap data yang diperoleh menunjukkan bahwa kapabilitas quality menjadi basis bagi kapabilitas delivery, yang juga menjadi basis bagi kapabilitas flexibility dan cost. Apakah kapabilitas tersebut dicapai secara eksklusif ataupun secara simultan, terlihat adanya keterkaitan dengan implementasi sejumlah program peningkatan tertentu. Pola umum dari akumulasi kapabilitas tersebut dapat digunakan untuk mengestimasi perilaku potensial maupun cara kerja yang lebih inovatif. Keywords: manufacturing capabilities, strategic manufacturing, resource-based view
De-industrialization in West Germany

This paper provides a theoretical framework and some empirical information on the shift from industrialization to de-industrialization which typically occurs in the course of economic growth. It is suggested that such a non-monotonic structural change is caused by a diminishing productivity bias and a constant demand bias between the industrial sector and other sectors of the economy. This pattern could be covered by short-term reversals of relative price trends resulting from external shocks. Hence, the recent gains of the industrial sector in West Germany are interpreted as a temporary deviation from the long-term trend of de-industrialization.
Extraction, purification and characterization of peroxidase from Vitis vinifera wastes

The proper consumption of plant wastes could not only provide a possible source of natural products, but it also is an environmental friendly research. The aim of this study was to use grape wastes as a source of peroxidase. In practice, one isoenzyme of peroxidase (POD1) was partially purified from Vitis vinifera wastes, the plant which is widely harvested in Iran. The activity of this novel peroxidase was determined using guaiacol as its substrate. The new peroxidase was partially purified and its kinetic parameters determined. The values of Km and Vmax of peroxidase for guaiacol were 83.2 mM and 0.35 M/min respectively. Optimum pH and temperature were determined for guaiacol to be 6.2 and 60°C respectively. According to SDS-PAGE results, the molecular weight of isozymes was 38-40 KD. The results indicate that agricultural leftovers from Vitis vinifera are a considerable source for a peroxidase with reliable kinetic behaviors.
Metabolic surgery in patients over 60 years old: short- and long-term results

Abstract Purpose: Laparoscopic Roux-en-Y gastric bypass can treat obesity related comorbidities and can prolong life expectancy. It remains unclear whether this type of surgery is also indicated in obese patients with advanced age. Materials and methods: In this retrospective monocentric study, we investigated the morbidity and outcomes of weight and metabolic control of bariatric surgery in patients older than 60 years and compared these findings with those of younger patients. Results: At 18 months after RY gastric bypass, weight losses of respectively 30 ± 11% and 34 ± 9% of total initial body weight were measured in the older and younger patients (p < 0.05). After 12 months, HbA1c dropped below 6.5% in 89% of patients younger and in 81% of patients older than 60 (p = 0.11). There was no mortality in either group, but there were significantly more complications and there was a longer hospital stay in the older patients. Conclusion: RY gastric bypass comes with a significantly higher morbidity and hospital stay in older patients, but weight loss and improvement of DM are similar as in the younger patients.
Neutron cryo-crystallography captures the protonation state of ferryl heme in a peroxidase

Peroxidase proton placement Heme enzymes catalyze a variety of biochemical oxidations through the activation of oxygen by iron. Casadei et al. used neutron crystallography to elucidate the mechanism of cytochrome c peroxidase (see the perspective by Groves and Boaz). In the highly reactive intermediate state termed compound I, the iron(IV) oxo, or ferryl, fragment was not protonated, whereas a nearby histidine residue was protonated. The sensitivity of neutron scattering to proton locations revealed these protonation states, where more common techniques, such as x-ray diffraction, have yielded more ambiguous results. Science, this issue p. 193; see also p. 142 The sensitivity of neutron scattering to proton locations clarifies the acid/base chemistry of a biochemical oxidation. [Also see Perspective by Groves and Boaz] Heme enzymes activate oxygen through formation of transient iron-oxo (ferryl) intermediates of the heme iron. A long-standing question has been the nature of the iron-oxygen bond and, in particular, the protonation state. We present neutron structures of the ferric derivative of cytochrome c peroxidase and its ferryl intermediate; these allow direct visualization of protonation states. We demonstrate that the ferryl heme is an Fe(IV)=O species and is not protonated. Comparison of the structures shows that the distal histidine becomes protonated on formation of the ferryl intermediate, which has implications for the understanding of O–O bond cleavage in heme enzymes. The structures highlight the advantages of neutron cryo-crystallography in probing reaction mechanisms and visualizing protonation states in enzyme intermediates.