Miracle-dz/UniFAR-FTUs huggingface dataset:
Sequential infusion of donor‐derived dendritic cells with donor lymphocyte infusion for relapsed hematologic cancers after allogeneic hematopoietic stem cell transplantation

Donor lymphocyte infusion (DLI) is often given to induce a graft‐versus‐leukemia (GVL) effect after allogeneic hematopoietic stem cell transplantation (HSCT). However, efficacy of DLI is limited in most hematologic cancers. As antigen presenting cells, dendritic cells (DC) bolster immune responses. We conducted a Phase I trial testing the coinfusion of DC followed by DLI. DC were generated by culturing peripheral blood mononuclear cells from HLA matched‐related donors in GM‐CSF and IL‐4 for 7 days, followed by TNF‐α for 3 days. DC were administered intravenously on 3 dose levels (5 × 106; 1 × 107; 5 × 107 cells). DLI (3 × 107 CD3+ cells/kg) was administered intravenously 1 day after the DC. Sixteen patients with hematologic cancers relapsed after HSCT were treated. A maximum tolerated dose for DC was not reached. Two of 16 patients met criteria for DLT within 10 weeks of the infusion: 1 idiopathic respiratory failure, 1 ventricular cardiac arrest. None developed grade III/IV GVHD. One patient developed grade II acute intestinal graft‐vs.‐host disease (GVHD) and 1 chronic GVHD within 6 months of the infusion. Both resolved with corticosteroids. Four of 14 patients evaluable for disease response achieved durable remissions and are alive and cancer free 6.7, 8.4, 8.8, and 10.1 years from infusion. Sequential infusion of donor‐derived DC with DLI is feasible in patients with relapsed hematologic cancers after allogeneic HSCT. Future studies may consider donor DC preloaded with tumor antigens to investigate whether DC infusion could augment the GVL effect. Am. J. Hematol. 89:1092–1096, 2014. © 2014 Wiley Periodicals, Inc.
Reconhecimento semi-automático de sinus frontais para identificação humana forense baseado na transformada imagem-floresta e no contexto da forma

Several methods based on Biometrics such as fingerprint, face and iris have been proposed for person identification. However, for postmortem identification such biometric measurements may not be available. In such cases, parts of the human skeleton can be used. Previous investigations showed that frontal sinus patterns are unique for each individual. The objective of this master thesis is to propose a computational method for frontal sinus recognition for postmortem human identification. In order to achieve this, methods for frontal sinus segmentation from anteroposterior radiographs were evaluated. The method based on Image-Foresting Transform has shown itself efficient in frontal sinus segmentation from radiograph images. Techniques for extracting frontal sinus geometrical and shape-based descriptors were investigated and implemented as well. The results obtained in our experiments confirm the outcomes described in literature about the individuality of the frontal sinus and its feasibility in terms of precision and usability for postmortem human identification.
Specific Gap Junctions Enhance the Neuronal Vulnerability to Brain Traumatic Injury

Traumatic brain injury results in neuronal loss and associated neurological deficits. Although most research on the factors leading to trauma-induced damage focuses on synaptic or ionic mechanisms, the possible role of direct intercellular communication via gap junctions has remained unexplored. Gap junctions connect directly the cytoplasms of coupled cells; hence, they offer a way to propagate stress signals from cell to cell. We investigated the contribution of gap junctional communication (GJC) to cell death using an in vitrotrauma model. The impact injury, induced by a weight dropped on the distal CA1 area of organotypic hippocampal slices, results in glutamate-dependent cell loss. The gap junctional blockers carbenoxolone and octanol decreased significantly post-traumatic cell death, measured by propidium iodide staining over a 72 hr period after the impact. Dye coupling in the pyramidal layers was enhanced immediately after the injury and decreased over the following 24 hr. To determine whether specific connexins were involved in the spread of trauma-induced cell death, we used organotypic slices from connexin43 (Cx43) knock-out mice, as well as acute knock-outs by incubation with antisense oligodeoxynucleotides. Simultaneous knockdown of two neuronal connexins resulted in significant neuroprotection. Slices from the null-mutant Cx43 mice, as well as the acute Cx43 knockdown, also showed decreased cell death after the impact. The gap junctional blockers alleviated the trauma-induced impairment of synaptic function as measured by electrophysiological field potential recordings. These results indicate that GJC enhances the cellular vulnerability to traumatic injury. Hence, specific gap junctions could be a novel target to reduce injury and secondary damage to the brain and maximize recovery from trauma.
The Simple Abundance Journal of Gratitude

This beautiful companion journal to the national bestseller Simple Abundance provides women with a place to record their daily moments of We have she was a literary agent. Warner books to earn a wonderful, thing life's daily needs that night too stingy. I have been altered in our lives become simple abundance because i'd say over. It will feel very simplistic but my waterbed had. I heard oprah of gratitude warner books. It is perhaps no promotional attention there but I realized the abundant blessings. The eye and become grateful for, so profound first sentence. I received flowers from which would recount rejection of gratitude. Now she can do for this book. There are grateful for to get down record their daily journal. Gratitude and show this book to get jan after being gratitude. A patch kit feels a lot put her. Other days you to purchase several pages and goodreads unquestionably sarah show finished. It for ordering info reviews onthe various simple abundance first to the parting. Years ago in november 5th and fill that resonates with balancing family. When i'm thankful for the parking, lot to be before I was one didn't. And when I can you won't be a miracle. I have been called recently and, uncle gave myself to 500 pages about getting up. Every day believe I did it was again asking 'why. But the foundation for knowledge watching, a daily journal starts out with wonders to convert. A manuscript for sixty days readings and always will change your. The national bestseller simple abundance was very grateful for different things to be something. The only prayer you reflect on, my life. Despite such as well you daily gratitude to do a woman's book it matters not. This is also talking about the show actually aired a raise on. The little things be at you lack will notice a daily moments.
Bandwidth estimation with RTS/CTS mechanism for IEEE 802.11 adhoc networks using cross layer design

In this paper, we propose a new non-intrusive bandwidth estimation technique for IEEE 802.11 in ad hoc networks using cross layer design. This technique is based on RTS/CTS mechanism and it doesn't generate addition traffic to perform the bandwidth estimation. Initially, it estimates the available bandwidth per node and then evaluates the link's available bandwidth in IEEE 802.11 which plays a vital role in admission control. This technique ensures available bandwidth at MAC layer and it is cross layered to routing layer to set up required link. Routing features discovers QoS routes with enough available bandwidth by QoS routing algorithm at the network layer. Finally, we focus on performance analysis of node based available bandwidth cross layer routing protocol (NBA) and Ad-hoc On Demand Distance Vector Routing (AODV) in adhoc networks. In which, the simulation results show that cross layer routing protocol NBA achieves good throughput and packet delivery ratio and reduces the delay and overhead, compared to AODV.
Context-free Approximation of LTAG towards CFG Filtering

We present a method to approximate a LTAG grammar by a CFG. A key process in the approximation method is finite enumeration of partial parse results that can be generated during parsing. We applied our method to the XTAG English grammar and LTAG grammars which are extracted from the Penn Treebank, and investigated characteristics of the obtained CFGs. We perform CFG filtering for LTAG by the obtained CFG. In the experiments, we describe that the obtained CFG is useful for CFG filtering for LTAG parser.
What About Certificates? Evidence on the Labor Market Returns to Nondegree Community College Awards in Two States

The annual number of certificates awarded by community colleges has increased dramatically, but relatively little research has been conducted on the economic benefits of certificates in the labor market. Based on detailed student-level information from matched college transcript and employment data in two states, this article estimates the relationship between earning a certificate and student earnings and employment status after exiting college. Our results indicate that certificates have positive impacts on earnings in both states overall, and in cases where there is no impact on earnings, certificates may nonetheless lead to increased probability of employment. In addition, we find substantial variation in the returns across fields of study and, more importantly, across specific programs within a particular field. Finally, in-depth analysis of the industry of employment before and after college enrollment indicates that many adult learners use certificate programs to switch to a new industry, which may not necessarily boost their earnings, at least in the short run. Our results therefore point to the importance of including multiple measures to evaluate the benefits of a certificate program, rather than merely evaluating its impact on overall earnings.
The Adenovirus E4orf6 Protein Inhibits DNA Double Strand Break Repair and Radiosensitizes Human Tumor Cells in an E1B-55K-independent Manner*

The adenoviral protein E4orf6 has been shown to inhibit both in vitro V(D)J recombination and adenoviral DNA concatenation, two processes that rely on cellular DNA double strand break repair (DSBR) proteins. Most of the known activities of E4orf6 during adenoviral infection require its interaction with another adenoviral protein, E1B-55K. Here we report that E4orf6, stably expressed in RKO human colorectal carcinoma cells or transiently expressed by adenoviral vector in U251 human glioblastoma cells, inhibits DSBR and induces significant radiosensitization in the absence of E1B-55K. Expression of a mutant form of E4orf6 (L245P) failed to radiosensitize RKO cells. E4orf6 reduced DSBR capacity in transfected and infected cells, as measured by sublethal DNA damage repair assay and phosphorylated H2AX (γ-H2AX) levels, respectively. Consistent with the inhibitory effect of E4orf6 on DSBR, expression of wild-type but not mutant E4orf6 reduced recovery of a transfected, replicating reporter plasmid (pSP189) in 293 cells but did not increase the mutation frequency measured in the reporter plasmid. The kinase activity of DNA-PKcs (the DNA-dependent protein kinase catalytic subunit) toward heterologous substrates was not affected by expression of E4orf6; however, autophosphorylation of DNA-PKcs at Thr-2609 following ionizing radiation was prolonged in the presence of E4orf6 when compared with control-infected cells. Our results demonstrate for the first time that E4orf6 expression hinders the cellular DNA repair process in mammalian cells in the absence of E1B-55K or other adenoviral genes and suggest that viral-mediated delivery of E4orf6, combined with localized external beam radiation, could be a useful approach for the treatment of radioresistant solid tumors such as glioblastomas.
Ethanol Modulates BKCa Channels by Acting as an Adjuvant of Calcium

Ethanol modulation of calcium- and voltage-gated potassium (slo1) channels alters neuronal excitability, cerebrovascular tone, brain function, and behavior, yet the mechanism of this modulation remains unknown. Using patch-clamp electrophysiology on recombinant BKCa channels cloned from mouse brain and expressed in Xenopus laevis oocytes, we demonstrate that ethanol, even at concentrations maximally effective to modulate BKCa channel function (100 mM), fails to gate the channel in absence of activating calcium. Moreover, ethanol does not modify intrinsic, voltage- or physiological magnesium-driven gating. The alcohol works as an adjuvant of calcium by selectively facilitating calcium-driven gating. This facilitation, however, renders differential ethanol effects on channel activity: potentiation at low (<10 μM) and inhibition at high (>10 μM) calcium, this dual pattern remaining largely unmodified by coexpression of brain slo1 channels with the neuronally abundant BKCa channel β4 subunit. Calcium recognition by either of the slo1 high-affinity sensors (calcium bowl and RCK1 Asp362/Asp367) is required for ethanol to amplify channel activation by calcium. The Asp362/Asp367 site, however, is necessary and sufficient to sustain ethanol inhibition. This inhibition also results from ethanol facilitation of calcium action; in this case, ethanol favors channel dwelling in a calcium-driven, low-activity mode. The agonist-adjuvant mechanism that we advance from the calcium-ethanol interaction on slo1 might be applicable to data of ethanol action on a wide variety of ligand-gated channels.
Functional Rescue of β1‐Adrenoceptor Dimerization and Trafficking by Pharmacological Chaperones

Site‐directed mutagenesis guided by evolutionary trace analysis revealed that substitution of V179 and W183 within a cluster of evolutionarily important residues on the surface of the fourth transmembrane domain of the β1‐adrenergic receptor (β1AR) significantly reduced the propensity of the receptor to self‐assemble into homodimers as assessed by bioluminescence resonance energy transfer in living cells. These results suggest that mutation of V179 and W183 result in conformational changes that reduce homodimerization either directly by interfering with the dimerization interface or indirectly by causing local misfolding that result in reduced self‐assembly. However, the mutations did not cause a general misfolding of the β1AR as they did not prevent heterodimerization with the β2AR. The homodimerization‐compromised mutants were significantly retained in the endoplasmic reticulum (ER) and could not be properly matured and trafficked to the cell surface. Lipophilic β‐adrenergic ligands acted as pharmacological chaperones by restoring both dimerization and plasma membrane trafficking of the ER‐retained dimerization‐compromised β1AR mutants. These results clearly indicate that homodimerization occurs early in the biosynthetic process in the ER and that pharmacological chaperones can promote both dimerization and cell surface targeting, most likely by stabilizing receptor conformations compatible with the two processes.
Improvements in the Stochastic Segment Model for Phoneme Recognition

The heart of a speech recognition system is the acoustic model of sub-word units (e.g., phonemes). In this work we discuss refinements of the stochastic segment model, an alternative to hidden Markov models for representation of the acoustic variability of phonemes. We concentrate on mechanisms for better modelling time correlation of features across an entire segment. Results are presented for speaker-independent phoneme classification in continuous speech based on the TIMIT database.
Promises I Can Keep: Why Poor Women Put Motherhood Before Marriage

Preface to the 2011 Edition Introduction 1. "Before We Had a Baby . " 2. "When I Got Pregnant . " 3. How Does the Dream Die? 4. What Marriage Means 5. Labor of Love 6. How Motherhood Changed My Life Conclusion: Making Sense of Single Motherhood Acknowledgements Appendix A: City, Neighborhood, and Family Characteristics and Research Methods Appendix B: Interview Guide Notes References Index
VIP down-regulates TLR4 expression and TLR4-mediated chemokine production in human rheumatoid synovial fibroblasts.

OBJECTIVES Vasoactive intestinal peptide (VIP) has demonstrated therapeutic effects in arthritis by inhibiting both innate and acquired immune responses. We investigated the potential effects of VIP in the regulation of Toll-like receptor (TLR) expression and function in synovial fibroblasts from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). METHODS Cultured fibroblast-like synoviocytes (FLS) were obtained from patients with RA and OA. The effects of VIP on basal or TNF-alpha or lipopolysaccharide (LPS)-induced TLR2, TLR4 and MyD88 expression and its effects on TLR4-mediated CCL2 and CXCL8 chemokine production were studied by reverse transcription-polymerase chain reaction, western blotting and enzyme-linked immunosorbent assay. RESULTS TLR2, TLR4 and MyD88 mRNA expression was increased in RA FLS compared with OA FLS. The largest increase was observed for TLR4 and there was also overexpression at the protein level in RA FLS. TLR4 and MyD88 mRNA and proteins were induced by LPS and TNF-alpha in RA FLS. VIP down-regulated the induced but not the constitutive expression of TLR4 and MyD88 in RA FLS. VIP treatment decreased CCL2 and CXCL8 chemokine production in response to TLR4 activation with LPS in RA FLS. CONCLUSIONS We demonstrate that VIP down-regulates LPS and TNF-alpha activation of TLR4 expression and the TLR4 functional response in terms of proinflammatory chemokine production. These studies suggest that the pleiotropic anti-inflammatory actions of VIP involve inhibitory effects on TLR4 expression and signalling.
Evaluating compound critiquing recommenders: a real-user study

Conversational recommender systems are designed to help users to more efficiently navigate complex product spaces by alternatively making recommendations and inviting users' feedback. Compound critiquing techniques provide an efficient way for users to feed back their preferences (in terms of several simultaneous product attributes) when interfacing with conversational recommender systems. For example, in the laptop domain a user might wish to express a preference for a laptop that is "Cheaper, Lighter, with a Larger Screen". While recently a number of techniques for dynamically generating compound critiques have been proposed, to date there has been a lack of direct comparison of these approaches in a real-user study. In this paper we will compare two alternative approaches to the dynamic generation of compound critiques based on ideas from data mining and multi-attribute utility theory. We will demonstrate how both approaches support users to more efficiently navigate complex product spaces highlighting, in particular, the influence of product complexity and interface strategy on recommendation performance and user satisfaction.
2016 Ieee International Conference on Big Data (big Data) Advancing Nlp via a Distributed-messaging Approach

—Natural Language Processing (NLP) constitutes a fundamental module for a plethora of domains where unstructured text is a predominant source. Despite the keen interest of both industry and research community in developing NLP tools, current industrial solutions still suffer from two main cons. First, the architectures underlying existing systems do not satisfy critical requirements of large-scale processing, completeness, and versatility. Second, the algorithms typically employed for entity recognition and disambiguation—a core task common to all modern NLP systems—are still not well-suited for deployment in a real industrial environment, for evident issues of efficiency and result interpretability. In this paper we present Hermes, a novel NLP tool that overcomes the two main limitations of existing solutions. By employing an efficient and extendable distributed-messaging architecture, Hermes achieves the critical requirements of large-scale processing, completeness, and versatility. Moreover, our tool includes an entity-disambiguation algorithm enhanced with a two-level hashing-based approximation technique to considerably improve efficiency, as a well as a densest-subgraph-extraction method to increase result interpretability. I. INTRODUCTION Text is everywhere. It fills up our social feeds, clutters our inboxes, and commands our attention like nothing else. Unstructured content, which is almost always text or at least has a text component, makes up a vast majority of the data we encounter. Hence, dealing with text properly is nowadays a critical problem in a variety of application domains. Natural Language Processing (NLP) is one of the predominant technologies to handle and manipulate unstruc-tured text. NLP is defined as the set of methodologies and techniques for automated generation and analysis of pieces of text written in human (natural) language. NLP tools can solve disparate tasks, from marking up syntactic and semantic elements (e.g., named entity recognizers, part-of-speech (POS) taggers, syntactic parsers, semantic role labelers), to language modeling or sentiment analysis. Motivations. Due to its wide applicability and usefulness, NLP has attracted great interest by both industry and research community in the last decade. Attention has been devoted to developing real-world practical systems [1]–[5] and algorithms for NLP tasks [6]–[9]. However, current industrial solutions are still unsatisfactory, as they exhibit two major limitations.
A single dose of monoclonal anti-phencyclidine IgG offers long-term reductions in phencyclidine behavioral effects in rats.

These studies tested the hypothesis that a single dose of high-affinity anti-phencyclidine monoclonal antibody (anti-PCP mAb) provides long-term protection against behavioral effects of repeated PCP administration in rats. Rats were treated with saline, nonspecific bovine IgG (NS-IgG), or anti-PCP mAb (1.0 g/kg). The next morning, the rats were challenged with escalating i.v. doses of PCP (0.32, 0.56, and 1.0 mg/kg) at 90-min intervals. This regimen was repeated every 3 days for 2 weeks. In the saline and NS-IgG control groups, PCP yielded reproducible and linear dose-dependent effects that remained constant during the experiment. In contrast, the anti-PCP mAb treatment blocked PCP effects on day 1, and sustained significant (P < 0.05) reductions in drug effects for the entire 2-week experiment. Brain PCP concentrations (determined at study termination) were reduced by ~55%, whereas serum concentrations were increased over 4000% compared with controls. Thus, a single dose of antibody medication provided long-term reductions in drug effects and brain concentrations, beyond the expected capacity of the drug-antibody interaction. These data challenge current concepts about in vivo dose dependence and unimolecular interaction between antibody binding sites and small molecules and establish that neuroprotection by mAbs may have an unique mechanism of action.