Miracle-dz/UniFAR-FTUs huggingface dataset:
Biochemical and Biophysical Analyses of Recombinant Forms of Human Topoisomerase I (*)

Amino acid sequence comparisons of human topoisomerase I (Topo I) with seven other cellular Topo I enzymes reveal that the enzyme can be divided into four major domains: the unconserved NH-terminal domain (24 kDa), the conserved core domain (54 kDa), a poorly conserved linker region (5 kDa), and the highly conserved COOH-terminal domain (8 kDa), which contains the active site tyrosine. To investigate this predicted domain organization, recombinant baculoviruses were engineered to express the 91-kDa full-length enzyme, a 70-kDa NH-terminally truncated enzyme that is missing the first 174 residues, and a 58-kDa NH- and COOH-terminally truncated core fragment encompassing residues 175-659. The specific activity of the full-length and Topo70 enzymes are indistinguishable from the native human Topo I purified from HeLa cells. Each protein is inhibited by camptothecin, topotecan, and 9-amino-camptothecin, but not by ATP. Activity is stimulated by Mg, Ba, Ca, Mn, spermine, and spermidine. The magnitude of the stimulatory effect of Mg is inversely proportional to the salt concentration. Furthermore, at KCl concentrations of 300 mM or greater, the addition of Mg is inhibitory. The effects of Mg and the polycations spermine and spermidine are partially additive, an indication that the stimulatory mechanisms of the two substances are different. Activity was strongly inhibited or abolished by Ni, Zn, Cu, Cd, and Co. An examination of the hydrodynamic properties of full-length Topo I, Topo70, and Topo58 demonstrates that the core, linker, and COOH-terminal domains fold into a globular structure, while the NH-terminal domain is highly extended. A comparison of the circular dichroism spectra of full-length Topo I and Topo70 demonstrates that residues 1-174 (21 kDa) of Topo I are largely if not completely unfolded. This observation is consistent with the fact that the NH-terminal domain is dispensable for activity.
The killer cell lectin‐like receptor B1 (KLRB1) 503T>C polymorphism (rs1135816) and acute rejection after liver transplantation

The killer cell lectin‐like receptor B1 (KLRB1) gene encodes for CD161 expressed by different subsets of leukocytes involved in the development of acute liver transplant rejection. The single nucleotide polymorphism (SNP) 503T>C (rs1135816) in the KLRB1 gene represents a missense mutation modifying functional properties of CD161. The aim of our study is to determine whether the SNP 503T>C is associated with acute liver transplant rejection. We genotyped the SNP for 163 liver recipients without acute rejection, 125 recipients with a single acute rejection, and 53 recipients with multiple acute rejections. The genotype frequencies within the groups did not show any significant difference. Our data suggest that the SNP 503T>C has no impact on the susceptibility of acute liver transplant rejection.
Circularly Polarized Antenna With Steerable Dipole-Like Radiation Pattern

An omnidirectional circularly polarized antenna with a rotatable dipole-shaped radiation pattern is proposed. The antenna is realized using a back-to-back coupled microstrip patch arrangement. The pattern is rotated by means of a phase shift, enabling reception (or transmission) of signals from all angles around a sphere. The method enables continuous pattern steering without the need for semiconductor or MEMS components integrated into the antenna. It also allows the use of more than one radiation pattern simultaneously, potentially providing coverage for any spherical angle. The theory is supported by simulation and measurement of four typical antenna configurations. The maximum gains are between 1.9 and 4 dBic and average axial-ratio varies between 2.5 and 3.65 dB at 2.47 GHz. The impedance bandwidth is from 2.4 to 2.51 GHz and the reconfigurable circular polarization is achieved from 2.464 to 2.484 GHz.
Epigenetic Temporal Control of Mouse Hox Genes in Vivo

www.sciencemag. org (this information is current as of July 6, 2009 ): The following resources related to this article are available online at http://www.sciencemag. org/cgi/content/full/324/5932/1320 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag. org/cgi/content/full/324/5932/1320/DC1 can be found at: Supporting Online Material http://www.sciencemag. org/cgi/content/full/324/5932/1320#otherarticles , 11 of which can be accessed for free: cites 28 articles This article http://www.sciencemag. org/cgi/collection/molec_biol Molecular Biology : subject collections This article appears in the following http://www.sciencemag. org/about/permissions.dtl in whole or in part can be found at: this article permission to reproduce of this article or about obtaining reprints Information about obtaining
The Study of Pricing Mechanism For Short Life Cycle Product Under Strategic Consumer Behavior

In this paper,we use Stachelberg game theory model to consider how a monopolistic firm of short life cycle product manages the revenue in the perspective of the strategic consumer behavior.The conclusion shows that the firm will leave a lot of money on the table if it ignores the consumer's strategic behavior.The firm should make the optimal price policy based on the inventory and consumers' expectation.When the inventory is small,the firm can ignore the strategic consumer behavior and make a high price policy;When the inventory is relative large,the firm's optimal profit will be realized under the only rational equilibrium;when the inventory is enough,the firm's optimal price policy is related with the consumer's rational expectation and value discount.The firm can use suitable inventory controlling to enhance the risk of stock out,and avoid the adverse impact of the consumer's strategic behavior.The conclusion is useful for the revenue management of the short life cycle product.
Mutational analysis of the TCOF1 gene in 11 Japanese patients with Treacher Collins Syndrome and mechanism of mutagenesis

Treacher Collins Syndrome (TCS) (OMIM 154500) is a congenital, craniofacial disorder inherited as an autosomal dominant trait. The responsible gene for TCS, TCOF1, was mapped to 5q32‐33.1 and identified in 1996. Since then, TCOF1 mutations in patients with TCS have been reported from Europe, North and South America, however, no TCS cases from an Asian country have been molecularly characterized. Here we report mutational analysis for 11 Japanese patients with TCS for the first time, and have identified TCOF1 mutations in 9 of them. The mutations detected were various, but most likely all the mutations are predicted to result in a truncated gene product, known as treacle. One mutation frequently reported was included in our cases, but no missense mutations were detected. These findings are similar to those for the previous studies for TCS in other races. We have speculated about the molecular mechanisms of the mutations in most cases. Collectively, we have defined some of the characteristic molecular features commonly observed in TCS patients, irrespective of racial difference. © 2005 Wiley‐Liss, Inc.
Nonergodicity parameters of colloidal glasses.

Nonergodicity parameters, the nondecaying components of the coherent intermediate scattering functions, of «hard-sphere» colloidal glasses were measured by dynamic light scattering. These agree reasonably well with the predictions of mode-coupling theory for hard-sphere atoms, with no adjustable parameters in the comparison. In addition, we provide further verification of the recently developed theory of dynamic light scattering by nonergodic media
Screening and rescreening for colorectal cancer. A controlled trial of fecal occult blood testing in 27,700 subjects

All inhabitants of the city of Göteborg who in 1982 were between 60 and 64 years of age (27,700) were randomly divided into a test and a control group. The 13,759 subjects in the test group were invited to perform Hemoccult II (Smith Kline Diagnostic, Sunnyvale, CA) fecal occult blood testing over 3 days and to repeat the testing after 16 to 22 months. At the first screening 9,040 (66%) completed the test, and 7,770 (58%) completed the test at the second screening. In the first screening the test group was divided into two subgroups in which the tests were rehydrated and unhydrated before development. All tests were rehydrated in the second screening; 1.9% and 5.8% of the tests were positive in the unhydrated and rehydrated subgroups, respectively. The number of diagnosed neoplasms in the first screening was significantly larger (P < 0.01) in the rehydrated group compared to the unhydrated group, 50 and 24 neoplasms, respectively. Sixteen of 61 carcinomas in the test group were found in the interval between the two screenings, 19 of the carcinomas at the second screening, and ten among the nonresponders. Rehydration of the Hemoccult II test is a necessity. Significantly more carcinomas (61) were found in the test group compared to the control group (20). There was a trend toward favorable tumor staging in the test group compared to the control group.
Rank–size distribution and spatio-temporal dynamics of tourist flows to China’s cities

This article investigates the rank–size distribution and spatio-temporal dynamics of domestic and inbound tourist flows in Chinese cities using Zipf’s law and the rank clock method. The results show that Zipf’s law captures key characteristics of the rank–size distribution of tourist flows to China’s cities. There are large differences between the inbound and domestic tourist flows not only for the top 100 but also for all cities. Three types of Zipf’s law are found to apply to China’s subregions. At the individual city level, most rank changes of the top 100 inbound and domestic tourist arrival cities appear in the lower part of the rank. The average rank shift distances of the top 100 inbound and domestic tourist arrival cities are 6.122 and 6.006, respectively. The dynamics of the rank–size distribution of domestic tourist flows to the top 100 cities are more stable than those of inbound tourist flows.
Normal p50 gating in unmedicated schizophrenia outpatients.

OBJECTIVE The hypothesis of a sensory gating defect in schizophrenia has been supported by studies demonstrating deficient auditory P50 gating in patients. P50 gating is the relative attenuation of P50 amplitude in the auditory evoked potential following the second auditory stimulus of a stimulus pair. METHOD Auditory evoked potentials of 12 unmedicated male patients with schizophrenia and 24 healthy men were recorded during three runs of 40 click pairs. Three alternative waveform-processing strategies were used to analyze the data. RESULTS Regardless of strategy used, the differences between subject groups regarding P50 amplitude and gating were nonsignificant. CONCLUSIONS The P50 gating in the patient group was normal. The results do not support the concept of the P50 gating defect as a general trait marker of schizophrenia.
Selective subtemporal amygdalohippocampectomy for refractory temporal lobe epilepsy: operative and neuropsychological outcomes.

OBJECT The authors evaluated operative, neuropathological, and neuropsychological results after selective subtemporal amygdalohippocampectomy for refractory temporal lobe epilepsy in patients who were observed for at least 2 years after surgery. METHODS Twenty-six consecutive patients underwent selective subtemporal amygdalohippocampectomy for nonlesional, medically refractory temporal lobe epilepsy. Neuropsychological evaluation using the Wechsler Adult Intelligence Scale was done before surgery in all patients, 2 months after surgery in 24 patients, and at 2-year follow up in 19 patients. A verbal paired associates learning test was administered before surgery and 2 months after surgery in 19 patients. The data were compared between the 13 patients in whom the language-dominant hemisphere was surgically treated and the six patients in whom the language-nondominant hemisphere was treated. After surgery, 84% of the patients attained either Engel Class I or II seizure outcome. There were no permanent subjective complications except postoperative memory impairment in one patient. Neuropathological examination confirmed hippocampal sclerosis in 19 patients. No significant differences in IQ and verbal memory test scores were observed between the patients in whom the language-dominant hemisphere was treated and those in whom the language-nondominant hemisphere was treated. Significant postoperative increases in verbal IQ, performance IQ, and full-scale IQ were observed over time. No significant differences were found between pre- and postoperative verbal memory test scores, and no subjective visual field loss was marked in any patient. CONCLUSIONS Subtemporal selective amygdalohippocampectomy provides favorable surgical and neuropsychological outcomes and does not cause significant postoperative decline of verbal memory if performed on the language-dominant side.
Enhancing product quality of a process

Purpose – Data mining (DM) is used to improve the performance of manufacturing quality control activity, and reduces productivity loss. The purpose of this paper is to discover useful hidden patterns from fabric data to reduce the amount of defective goods and increase overall quality.Design/methodology/approach – This research examines the improvement of manufacturing process via DM techniques. The paper explores the use of different preprocessing and DM techniques (rough sets theory, attribute relevance analysis, anomaly detection analysis, decision trees and rule induction) in carpet manufacturing as the real world application problem. SPSS Clementine Programme, Rosetta Toolkit, ASP (Active Server Pages) and VBScript programming language are used.Findings – The most important variables of attributes that are effective in product quality are determined. A decision tree (DT) and decision rules are generated. Therefore, the faults in the process are detected. An on‐line programme is generated and the mode.
Extreme rituals in a BDSM context: the physiological and psychological effects of the ‘Dance of Souls’

Abstract Participation in extreme rituals (e.g., fire-walking, body-piercing) has been documented throughout history. Motivations for such physically intense activities include religious devotion, sensation-seeking and social bonding. The present study aims to explore an extreme ritual within the context of bondage/discipline, dominance/submission and sadism/masochism (BDSM): the ‘Dance of Souls’, a 160-person ritual involving temporary piercings with weights or hooks attached and dancing to music provided by drummers. Through hormonal assays, behavioural observations and questionnaires administered before, during and after the Dance, we examine the physiological and psychological effects of the Dance, and the themes of spirituality, connectedness, transformation, release and community reported by dancers. From before to during the Dance, participants showed increases in physiological stress (measured by the hormone cortisol), self-reported sexual arousal, self-other overlap and decreases in psychological stress and negative affect. Results suggest that this group of BDSM practitioners engage in the Dance for a variety of reasons, including experiencing spirituality, deepening interpersonal connections, reducing stress and achieving altered states of consciousness.
Identification of mycobacteria by PCR-based sequence determination of the 32-kilodalton protein gene

In this study, a part of the nucleotide sequence of the mycobacterial 32-kDa protein gene was determined by PCR-based sequencing. A total of 24 mycobacterial strains, representing 10 species, were studied. Sequences of all tested members of the Mycobacterium tuberculosis complex were identical to each other and to the previously published sequence of M. tuberculosis H37Rv. The sequences of M. avium and M. intracellulare were different from each other. MAIX strains, identified with the Gen-Probe MAIX test, had sequences identical to each other but clearly different from those of M. avium and M. intracellulare. Each of the other mycobacterial species investigated, i.e., M. kansasii, M. gastri, M. gordonae, and M. malmoense, had a unique species-specific sequence. These results demonstrate that there is variation in the nucleotide sequence of the 32-kDa protein gene among different mycobacterial species. Thus, we propose that this gene can be used for PCR-based identification of mycobacteria.
Risk-Return Analysis of Dynamic Investment Strategies

The investment management industry has developed such a wide range of trading strategies, that many investors feel lost when they have to choose the investment style that meets their requirements. Comparing these on a like-for-like basis is a difficult task about which much has been written. The scope of this paper is restricted to strategies investing in a single asset, and which are driven by the price of this asset. We show how those strategies can be fully characterised by two components: an option profile and some trading impact. The option profile depends solely on the final asset value, whereas trading impact is driven by the realised volatility. From this analysis, most of these investment strategies can be categorised in one of three families: directional, contrarian and trend-following. While directional strategies exhibit the same kind of behaviour as the underlying, contrarian and trend-following strategies exhibit asymmetric return distributions. Those asymmetric behaviours can be misleading at first sight, as a seemingly stable strategy may hide large potential losses.
Suppression of interleukin‐3‐induced gene expression by a C‐terminal truncated Stat5: role of Stat5 in proliferation.

Interleukin‐3 (IL3) was shown recently to utilize the transcription factor Stat5, but the genes regulated by this pathway and the biological consequence of Stat5 activation remained to be determined. In order to study the role of Stat5 in IL3 signalling, we constructed a dominant‐negative Stat5 protein by C‐terminal truncation, and inducibly expressed it in an IL3‐dependent cell line. The effect of dominant‐negative Stat5 induction on expression of IL3 early response genes was examined, and expression of several genes, including cis, osm and pim‐1 was inhibited profoundly. The expression of c‐fos was also reduced, but to a lesser extent. While activated Ras alone (though not Stat5 alone) could induce c‐fos, maximal expression required the action of both Ras and Stat5. Interestingly, although the membrane‐proximal region of the IL3 receptor beta‐chain is responsible for both Jak2‐Stat5 activation and c‐myc induction, c‐myc levels were not affected by the dominant‐negative Stat5. Thus, the signals directed by this membrane‐proximal domain, which is essential for transducing a DNA synthesis signal, can be separated further into Stat5 or c‐myc pathways. The net effect of dominant‐negative Stat5 expression was partial inhibition of IL3‐dependent growth. This provides the first direct evidence that Stat5 is involved in regulation of cell proliferation.
An Exact Method for Vehicle Routing and Truck Driver Scheduling Problems

In most developed countries working hours of truck drivers are constrained by hours of service regulations. When optimizing vehicle routes, trucking companies must consider these constraints to assure that drivers can comply with the regulations. This paper studies the combined vehicle routing and truck driver scheduling problem (VRTDSP), which generalizes the well-known vehicle routing problem with time windows by considering working hour constraints. A branch-and-price algorithm for solving the VRTDSP is presented. This is the first algorithm that solves the VRTDSP to proven optimality.
Rapid PKA‐catalysed phosphorylation of boar sperm proteins induced by the capacitating agent bicarbonate

In boar spermatozoa, the capacitating agent bicarbonate has been shown to induce rapid changes both in plasma membrane lipid architecture and in motility; in each case, a PKA‐dependent pathway is involved. Early bicarbonate‐induced changes in protein phosphorylation were probed using a commercial antibody against the phosphorylated form of the consensus substrate site for cyclic AMP‐dependent protein kinase. The antibody detected relatively few bands in sperm extracts, of which only a small number showed incubation‐dependent changes. While the quantitative response varied between boar ejaculates, in general terms bicarbonate induced phosphorylation increases in bands of 96, 64, and 59 kDa within 80 sec. The changes reached a maximum after about 160 sec, declined somewhat thereafter, and then increased again slowly as incubation progressed further (up to 21 min). The bicarbonate‐induced increases were strongly dependent on the presence of BSA in the incubation medium. They were inhibited by H89 (PKA inhibitor) but not by GF (PKC inhibitor), and were enhanced by papaverine (phosphodiesterase inhibitor) and by calyculin (protein phosphatase inhibitor). The cyclic AMP analogue cBIMPS was able to mimic bicarbonate action though its effect was less dramatic. Stearated Ht31, a permeable inhibitor of PKA's binding to A‐kinase anchoring protein, did not affect either the intensity or the specificity of the bicarbonate‐induced phosphorylation changes, though it blocked motility entirely. Immunocytochemical studies revealed marked bicarbonate‐dependent phosphorylation changes in the post‐acrosomal region of the head and in the neck, midpiece, and anterior regions of the tail. Fractionation of stimulated spermatozoa showed that all bands detectable with the antibody were bound to heads and to midpieces and associated large tail fragments; no bands were detected in either small tail or membrane fragments or in the cytoplasmic fraction. Differential extraction of the midpiece/large tail fraction revealed two protein bands with closely similar electrophoretic mobilities to the 96‐ and 59‐kDa phosphorylated bands; MALDI‐TOF analyses of these bands revealed both to be members of the Odf2 family. Mol. Reprod. Dev. 67: 337–352, 2004. © 2004 Wiley‐Liss, Inc.
An active clamp ZVT converter with input-parallel and output-series configuration

A novel converter is proposed in this paper to satisfy the high power, high step-up and isolated conversion requirements. In the proposed converter, the input-parallel configuration is adopted to share the large input current and reduce the conduction losses, while the output-series configuration is adopted to get a high voltage gain. Consequently, a transformer with a low turns ratio can be applied, which makes the transformer optimized easier. Moreover, the active clamp circuits are employed here to reduce the voltage stress of the switches and recycle the energy stored in the leakage inductance, and ZVT is achieved during the whole switching transition for all the active switches, so the switching losses can be reduced greatly. Furthermore, the diode reverse-recovery problem is partly solved due to the leakage inductance. Finally, a 40V-input 400V-output 1kW prototype is built to demonstrate the theoretical analysis, and the maximum efficiency of 94.9% and 93.3% at full load are achieved.