Miracle-dz/UniFAR-FTUs huggingface dataset:
Genesis and death of vocal control neurons during sexual differentiation in the zebra finch

Several song-related regions in the adult zebra finch brain have substantially more neurons in males than in females. Such differences appear to arise from sex differences in circulating steroids during early posthatch life. In the present study, developmental mechanisms involved in the production of sex differences are explored by examinations of the normal time course of posthatch neurogenesis and cell death in vocal control circuits. As a first step toward determining whether rates of neuron production may be different in males and females, tritiated thymidine, a marker of cell division, was administered to zebra finches at various times during the first month after hatching. Birds were sacrificed at 60 d. The number of cells formed after hatching and present at 60 d was then evaluated in 3 vocal control regions--HVc (hyperstriatum ventralis pars caudalis) and its 2 principal targets, RA (robust nucleus of the archistriatum) and Area X. Cell death was quantified by counts of normal and pyknotic, degenerating cells made in these nuclei in additional, untreated birds of both sexes at 5 d intervals from 5 to 45 d of age. The combined results of these experiments suggest that differential cell death is a major factor in the development of sex differences in the song control system and provide the first direct evidence for sex differences in cell death in the developing telencephalon. Although developmental time tables differ among the 3 brain areas examined, at specific ages significantly higher numbers of pyknotic cells were observed in HVc, RA, and presumptive Area X in females compared to males. Peak levels of cell death in RA occur 4–6 weeks after hatching. This is about 3 weeks after the onset of sex differences in steroid levels that, in turn, lead to differential organization of song system nuclei. This pattern of results suggests that designation for death and actual cell loss are temporally dissociated in this system. Neuron proliferation for HVc and Area X, but not RA, continues throughout the first 30 d after hatching, and a significant sex difference was found in the number of cells present in HVc at 60 d that were formed after hatching. Comparisons of the timing of cell death and cell incorporation suggest that this difference may be best accounted for by differential survival of neurons formed after hatching rather than differential rates of neuron production. Neither differential neurogenesis nor differential neuron death can fully account for the apparent extreme sexual dimorphism in the number of neurons in Area X. (ABSTRACT TRUNCATED AT 400 WORDS)
Interatrial septal thickness as a marker of structural and functional remodeling of the left atrium in patients with atrial fibrillation

Background/Aims: There have been reports that interatrial septal (IAS) thickness is increased in patients with atrial fibrillation (AF). This study was performed to investigate whether IAS thickness determined by transthoracic echocardiography (TTE) represents the amount of left atrium (LA) structural and functional remodeling. Methods: The study population consisted of 104 consecutive patients who underwent catheter ablation (CA) for AF (paroxysmal atrial fibrillation [PAF], 82; persistent atrial fibrillation [PeAF], 22). IAS thickness and left atrium volume (LAV) using TTE, and LA voltage (LAVOL) using 3-dimensional electroanatomical mapping system were assessed during sinus rhythm. Results: IAS thickness was significantly correlated with maximal LAV (LAVmax) (r = 0.288, p = 0.003), mean LAVOL (r = –0.537, p < 0.001), total left atrium emptying fraction (LAEFtotal; r = –0.213, p = 0.030), and active LAEF (LAEFactive; r = –0.249, p = 0.014). IAS thickness was greater in the high-risk group (≥ 2) compared to other groups according to CHA2DS2-VASc score (p = 0.019). During a follow-up of 19.6 months, 23 subjects (22.1%; PAF, 17; PeAF, 6) had recurrence of arrhythmia. Univariate analysis showed that LAVmax, minimal LAV, mean LAVOL, LVEFtotal, LVEFactive, and IAS thickness were associated with recurrence of arrhythmia. However, on multivariate analysis, only mean LAVOL and LAEFactive were independent risk factors for recurrence. Conclusions: Although IAS thickness showed significant correlations with parameters for LA structural and functional remodeling, this parameter alone could not independently predict recurrence of arrhythmia after CA for AF.
Accuracy of GFR estimation in obese patients.

BACKGROUND AND OBJECTIVES Adequate estimation of renal function in obese patients is essential for the classification of patients in CKD category as well as the dose adjustment of drugs. However, the body size descriptor for GFR indexation is still debatable, and formulas are not validated in patients with extreme variations of weight. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS This study included 209 stages 1-5 CKD obese patients referred to the Department of Renal Function Study at the University Hospital in Lyon between 2010 and 2013 because of suspected renal dysfunction. GFR was estimated with the Chronic Kidney Disease and Epidemiology equation (CKD-EPI) and measured with a gold standard method (inulin or iohexol) not indexed (mGFR) or indexed to body surface area determined by the Dubois and Dubois formula with either real (mGFRr) or ideal (mGFRi) body weight. Mean bias (eGFR-mGFR), precision, and accuracy of mGFR were compared with the results obtained for nonobese participants (body mass index between 18.5 and 24.9) who had a GFR measurement during the same period of time. RESULTS Mean mGFRr (51.6 ± 24.2 ml/min per 1.73 m(2)) was significantly lower than mGFR, mGFRi, and eGFRCKD-EPI. eGFRCKD-EPI had less bias with mGFR (0.29; -1.7 to 2.3) and mGFRi (-1.62; -3.1 to 0.45) compared with mGFRr (8.7; 7 to 10). This result was confirmed with better accuracy for the whole cohort (78% for mGFR, 84% for mGFRi, and 72% for mGFRr) and participants with CKD stages 3-5. Moreover, the Bland Altman plot showed better agreement between mGFR and eGFRCKD-EPI. The bias between eGFRCKD-EPI and mGFRr was greater in obese than nonobese participants (8.7 versus 0.58, P<0.001). CONCLUSIONS This study shows that, in obese CKD patients, the performance of eGFRCKD-EPI is good for GFR ≤ 60 ml/min per 1.73 m(2). Indexation of mGFR with body surface area using ideal body weight gives less bias than mGFR scaled with body surface area using real body weight.
Activation of the N-Ras–PI3K–Akt-mTOR Pathway by Hepatitis C Virus: Control of Cell Survival and Viral Replication

ABSTRACT The hepatitis C virus (HCV) replication complex is localized within detergent-resistant membranes or lipid rafts. We analyzed the protein contents of detergent-resistant fractions isolated from Huh7 cells expressing a self-replicating full-length HCV-1b genome. Using two-dimensional gel electrophoresis followed by mass spectrometry, we identified N-Ras as one of the proteins in which expression was increased in the detergent-resistant fractions from HCV genomic replicon clones compared to control cells. N-Ras is an activator of the phosphatidylinositol-3-kinase (PI3K)-Akt pathway. We found that the activities of PI3K and Akt, as well as the activity of their downstream target, mTOR, in the HCV-replicating cells were increased. Both PI3K-Akt- and mTOR-dependent pathways have been shown to promote cell survival. In agreement with this, HCV replicon cells were resistant to serum starvation-induced apoptosis. We also characterized the role of this pathway in HCV replication. Reduction of N-Ras expression by transfection of N-Ras small interfering RNA (siRNA) resulted in increased replication of HCV. We observed a similar increase in HCV replication in cells treated with the PI3K inhibitor LY294002 and in cells transfected with mTOR siRNA. Taken together, these data suggest that increased N-Ras levels in subcellular sites of HCV replication and stimulation of the prosurvival PI3K-Akt pathway and mTOR by HCV not only protect cells against apoptosis but also contribute to the maintenance of steady-state levels of HCV replication. These effects may contribute to the establishment of persistent infection by HCV.
Visualizing Reality in an Augmented Virtual Environment

An Augmented Virtual Environment (AVE) fuses dynamic imagery with 3D models. An AVE provides a unique approach to visualizing spatial relationships and temporal events that occur in real-world environments. A geometric scene model provides a 3D substrate for the visualization of multiple image sequences gathered by fixed or moving image sensors. The resulting visualization is that of a world-in-miniature that depicts the corresponding real-world scene and dynamic activities. This paper describes the core elements of an AVE system, including static and dynamic model construction, sensor tracking, and image projection for 3D visualization.
Multi-objective test problems, linkages, and evolutionary methodologies

Existing test problems for multi-objective optimization are criticized for not having adequate linkages among variables. In most problems, the Pareto-optimal solutions correspond to a fixed value of certain variables and diversity of solutions comes mainly from a random variation of certain other variables. In this paper, we introduce explicit linkages among variables so as to develop difficult two and multi-objective test problems along the lines of ZDT and DTLZ problems. On a number of such test problems, this paper compares the performance of a number of EMO methodologies having (i) variable-wise versus vector-wise recombination operators and (ii) spatial versus unidirectional recombination operators. Interesting and useful conclusions on the use of above operators are made from the study.
Gender and mode of assessment at university: should we assume female students are better suited to coursework and males to unseen examinations?1

This paper reports on research conducted at the University of Sussex and examines whether female students have a particular preference for coursework, and whether such a preference is a key factor in their current undergraduate success. The performances of 638 students on courses whose assessment modes comprised both coursework and examinations were analysed to determine what, if any, gender differences were evident in relation to performances on each elements. In order to supplement the quantitative findings, qualitative data elicited via two online surveys, and focusing on student perceptions of coursework and examinations and attitudes to undergraduate study, are also discussed. Our findings contribute to the debate about gender differences across modes of assessment, and in particular take issue with the claim that female students, by contrast with males, both favour and are favoured by the use of coursework as opposed to unseen examinations in mode of assessment arrays.
Travel motivation as a determinant of shopping venue

Purpose – Shopping is a popular tourist activity. While a person might not travel for the purpose of shopping, many tourists shop while traveling. This study aims to examine travel motivation as a predictor of the importance assigned to desired shopping center attributes for three different shopping centers, and their effect on satisfaction, and re‐patronage intention.Design/methodology/approach – Data were collected via mall‐intercept surveys from 624 tourist shoppers at seven shopping centers. A tourist was defined as a person who traveled a distance of at least 50 miles from their home.Findings – Desired shopping center attributes are influenced by travel motivation. For the superregional center, a linear relationship was computed for the exploration travel motive, mall environment, overall satisfaction and re‐patronage intention. Interestingly, overall satisfaction with the shopping center was not a significant predictor of re‐patronage intention in the theme/festival or super off‐price centers.Origin.
Response to phenelzine and imipramine in placebo nonresponders with atypical depression. A new application of the crossover design.

• We employed a study design that permitted a double-blind 12-week contrast of imipramine hydrochloride and phenelzine sulfate therapies in patients who met Columbia University criteria for atypical depression and were unresponsive to 7 weeks of treatment with placebo. These patients were found to benefit selectively from therapy with monoamine oxidase inhibitors compared with tricyclic drug therapy. This supports our observation about treatment response in depressed patients with reversed vegetative features. The design we utilized in this study has not previously been reported, to our knowledge. It was hypothesized that it would offer the advantage of the removal of a portion of placebo responders and serve to replicate our original findings. Treatment response to therapy with both imipramine and phenelzine in placebo nonresponders was uniformly lower (roughly 20% less than corresponding rates for patients who did not participate in the initial 6-week placebo trial). This is consistent with the view that the lower response rates were a result of the removal of some "placebo" responders in the drug groups. We think this is a useful design that should be considered in all studies of placebo and two active treatment regimens.
Proteolytic maturation of α2δ represents a checkpoint for activation and neuronal trafficking of latent calcium channels

The auxiliary α2δ subunits of voltage-gated calcium channels are extracellular membrane-associated proteins, which are post-translationally cleaved into disulfide-linked polypeptides α2 and δ. We now show, using α2δ constructs containing artificial cleavage sites, that this processing is an essential step permitting voltage-dependent activation of plasma membrane N-type (CaV2.2) calcium channels. Indeed, uncleaved α2δ inhibits native calcium currents in mammalian neurons. By inducing acute cell-surface proteolytic cleavage of α2δ, voltage-dependent activation of channels is promoted, independent from the trafficking role of α2δ. Uncleaved α2δ does not support trafficking of CaV2.2 channel complexes into neuronal processes, and inhibits Ca2+ entry into synaptic boutons, and we can reverse this by controlled intracellular proteolytic cleavage. We propose a model whereby uncleaved α2δ subunits maintain immature calcium channels in an inhibited state. Proteolytic processing of α2δ then permits voltage-dependent activation of the channels, acting as a checkpoint allowing trafficking only of mature calcium channel complexes into neuronal processes. DOI: http://dx.doi. org/10.7554/eLife.21143. 001
Estimation of the Domain of Attraction for Non-Polynomial Systems: A Novel Method

Abstract Until now, several methods have been developed to compute the domains of attraction for stationary points of polynomial non-linear systems. For the case of non-polynomial systems, though, this question is still open. In this paper a new method, based on Lyapunov's stability theory and the theorem of Ehlich and Zeller, is presented for the computation of domains of attraction of non-polynomial systems with quadratic Lyapunov functions. Unlike other methods, which use a polynomial approximation for the non-polynomial terms, we compute upper bounds on the interpolation error for each of the non-polynomial terms. Then, the theorem of Ehlich and Zeller is adapted to non-polynomial systems. Our method yields, for a given Lyapunov function, upper and lower bounds for the level curve enclosing the domain of attraction.
Generalized Probabilistic Decision-Based Neural Networks for texture classification and retrieval

For various applications, formulating texture features in distributional forms can sometimes provide meaningful representation than in numerical forms. In this paper, we first proposed a novel methodology for measuring the difference between two mixture Gaussian distributions. Based on the derived formula, a Generalized Probabilistic Decision-Based Neural Network (GPDNN) is then proposed and implemented to realize the difference measurement method. By constructing a two layered pyramid-type network structure, the proposed GPDNN receives data in distributional form via 2-D grid input nodes, and outputs the classification and/or retrieval results from the top layer node. Forty texture images are selected from the MIT Vision Texture (VisTex) database to evaluate the proposed GPDNN for the texture classification and retrieval. Experimental results show that (1) by using the proposed difference measurement methods, the texture pattern retrieval rates can be improved from 77% to 82%, compared with some published leading methods, and (2) the proposed GPDNN shows significant texture classification and retrieval performance, which are about 90:1% and 88:6% of the accuracy, and much better than the traditional methods, i.e., 82.2% and 79.9%, respectively.
Retinoblastoma and Its Binding Partner MSI1 Control Imprinting in Arabidopsis

Parental genomic imprinting causes preferential expression of one of the two parental alleles. In mammals, differential sex-dependent deposition of silencing DNA methylation marks during gametogenesis initiates a new cycle of imprinting. Parental genomic imprinting has been detected in plants and relies on DNA methylation by the methyltransferase MET1. However, in contrast to mammals, plant imprints are created by differential removal of silencing marks during gametogenesis. In Arabidopsis, DNA demethylation is mediated by the DNA glycosylase DEMETER (DME) causing activation of imprinted genes at the end of female gametogenesis. On the basis of genetic interactions, we show that in addition to DME, the plant homologs of the human Retinoblastoma (Rb) and its binding partner RbAp48 are required for the activation of the imprinted genes FIS2 and FWA. This Rb-dependent activation is mediated by direct transcriptional repression of MET1 during female gametogenesis. We have thus identified a new mechanism required for imprinting establishment, outlining a new role for the Retinoblastoma pathway, which may be conserved in mammals.
Face and Favor: The Chinese Power Game

Western research on interpersonal behavior patterns and rules of exchange needs to move beyond the assumption of isolated individuals socialized to make rational decisions on the basis of self-interest. A review of recent research shows that Chinese society and other similar societies follow rules that deviate from those of the West. In such societies, norms of reciprocity (bao) are intense, but these norms are heavily shaped by the hierarchically structured network of social relations (guanxi) in which people are embedded, by the public nature of obligations, and by the long time period over which obligations are incurred through a self-conscious manipulation of face and related symbols. These special cultural symbols, as well as the historical monopoly of valuable resources by powerful leaders, help explain he origin of these patterns. There are several forces leading to change but also some sources of continuity, which help maintain these patterns regardless of political and economic context.
Consuming work/productive leisure: the consumption patterns of second home environments

This paper, based on a research project on British second home owners in rural France, uses empirical evidence derived from ethnographic interviews to investigate and analyse the escapism opportunities afforded by the purchase and consumption of a second home in a foreign environment. Escape is the main theme, from pressure of work, everyday routine, from commodification, to a space which is a bolt-hole, a retreat or a genuine break from paramount reality. The actions and activities through which the escape is attempted or effected are examined, leading to some insights into the home-holiday as well as the production-consumption relationship. It seems that the French second home experience provides an example of an alternative to the routines and constraints of the world of work on the one hand and from commodified tourism products on the other. In committing to the project, which mingles production and consumption, owners believe they are achieving a balance which is missing from their lives in Britain, .
Psychological Distress and Mortality in Systolic Heart Failure

Background—Depression, anxiety, and type D (“distressed”) personality (tendency to experience negative emotions paired with social inhibition) have been associated with poor prognosis in coronary heart disease, but little is known about their role in chronic heart failure. Therefore, we investigated whether these indicators of psychological distress are associated with mortality in chronic heart failure. Method and Results—Consecutive outpatients with chronic heart failure (n=641; 74.3% men; mean age, 66.6±10.0 years) filled out a 4-item questionnaire to assess mixed symptoms of anxiety and depression and the 14-item type D scale. End points were defined as all-cause and cardiac mortality. After a mean follow-up of 37.6±15.6 months, 123 deaths (76 due to cardiac cause) were recorded. Cumulative hazard functions for elevated anxiety/depression symptoms differed marginally for all-cause (P=0.06), but not cardiac, mortality (P=0.43); type D personality was associated with neither all-cause mortality (P=0.63) nor cardiac mortality (P=0.87). In multivariable analyses, neither elevated anxiety/depression symptoms nor type D personality was associated with all-cause mortality (hazard ratio [HR]=1.18; 95% CI, 0.76 to 1.84; P=0.45 and HR=1.09; 95% CI, 0.67 to 1.77; P=0.73, respectively) or cardiac mortality (HR=1.13; 95% CI, 0.63 to 2.04; P=0.65 and HR=1.16; 95% CI, 0.62 to 2.18; P=0.67). In secondary analyses, a 1-point increase in anxiety/depression (range, 0 to 16) was associated with an 8% increase in risk for all-cause mortality (HR=1.08; 95% CI, 1.01 to 1.15; P=0.02). Conclusions—Neither elevated anxiety/depression symptoms nor type D personality was associated with an increased risk for all-cause or cardiac mortality. Future studies with adequate power and a longer follow-up duration are needed to further elucidate the role of psychological distress in chronic heart failure.
Cholesterol and Sphingomyelin Drive Ligand-independent T-cell Antigen Receptor Nanoclustering*

Background: The TCR forms nanoclusters in the plasma membrane independent of ligand binding. Results: Membrane cholesterol and sphingomyelin facilitate TCR nanoclustering, thereby enhancing the avidity toward the ligand. Conclusion: The membrane lipid composition regulates the degree of TCR nanoclustering and thus T-cell sensitivity. Significance: This work contributes to the understanding of the consequences of specific lipid-membrane protein interactions. The T-cell antigen receptor (TCR) exists in monomeric and nanoclustered forms independently of antigen binding. Although the clustering is involved in the regulation of T-cell sensitivity, it is unknown how the TCR nanoclusters form. We show that cholesterol is required for TCR nanoclustering in T cells and that this clustering enhances the avidity but not the affinity of the TCR-antigen interaction. Investigating the mechanism of the nanoclustering, we found that radioactive photocholesterol specifically binds to the TCRβ chain in vivo. In order to reduce the complexity of cellular membranes, we used a synthetic biology approach and reconstituted the TCR in liposomes of defined lipid composition. Both cholesterol and sphingomyelin were required for the formation of TCR dimers in phosphatidylcholine-containing large unilamellar vesicles. Further, the TCR was localized in the liquid disordered phase in giant unilamellar vesicles. We propose a model in which cholesterol and sphingomyelin binding to the TCRβ chain causes TCR dimerization. The lipid-induced TCR nanoclustering enhances the avidity to antigen and thus might be involved in enhanced sensitivity of memory compared with naive T cells. Our work contributes to the understanding of the function of specific nonannular lipid-membrane protein interactions.