Miracle-dz/UniFAR-FTUs huggingface dataset:
Detailed Morphological Changes of Foveoschisis in Patient with X-Linked Retinoschisis Detected by SD-OCT and Adaptive Optics Fundus Camera

Purpose. To report the morphological and functional changes associated with a regression of foveoschisis in a patient with X-linked retinoschisis (XLRS). Methods. A 42-year-old man with XLRS underwent genetic analysis and detailed ophthalmic examinations. Functional assessments included best-corrected visual acuity (BCVA), full-field electroretinograms (ERGs), and multifocal ERGs (mfERGs). Morphological assessments included fundus photography, spectral-domain optical coherence tomography (SD-OCT), and adaptive optics (AO) fundus imaging. After the baseline clinical data were obtained, topical dorzolamide was applied to the patient. The patient was followed for 24 months. Results. A reported RS1 gene mutation was found (P203L) in the patient. At the baseline, his decimal BCVA was 0.15 in the right and 0.3 in the left eye. Fundus photographs showed bilateral spoke wheel-appearing maculopathy. SD-OCT confirmed the foveoschisis in the left eye. The AO images of the left eye showed spoke wheel retinal folds, and the folds were thinner than those in fundus photographs. During the follow-up period, the foveal thickness in the SD-OCT images and the number of retinal folds in the AO images were reduced. Conclusions. We have presented the detailed morphological changes of foveoschisis in a patient with XLRS detected by SD-OCT and AO fundus camera. However, the findings do not indicate whether the changes were influenced by topical dorzolamide or the natural history.
A GIS-based fire spread simulator integrating a simplified physical wildland fire model and a wind field model

ABSTRACT This article discusses the integration of two models, namely, the Physical Forest Fire Spread (PhFFS) and the High Definition Wind Model (HDWM), into a Geographical Information System-based interface. The resulting tool automates data acquisition, preprocesses spatial data, launches the aforementioned models and displays the corresponding results in a unique environment. Our implementation uses the Python language and Esri’s ArcPy library to extend the functionality of ArcMap 10.4. The PhFFS is a simplified 2D physical wildland fire spread model based on conservation equations, with convection and radiation as heat transfer mechanisms. It also includes some 3D effects. The HDWM arises from an asymptotic approximation of the Navier–Stokes equations, and provides a 3D wind velocity field in an air layer above the terrain surface. Both models can be run in standalone or coupled mode. Finally, the simulation of a real fire in Galicia (Spain) confirms that the tool developed is efficient and fully operational.
Structure of the d(CGCGAATTCGCG)2 Complex of the Minor Groove Binding Alkylating Agent Alkamin Studied by Mass Spectrometry

Nitrogen mustard alkylating agents are important cancer drugs. Much interest has been focused on redirecting their covalent adducts from the N7 atoms of guanine in the major groove of DNA to the N3 atoms of adenine in the minor groove by attaching mustard groups to AT-selective minor groove binding ligands. Here we describe the use of electrospray ionization and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry to study the structure of the DNA complexes of two minor groove binding polybenzamide mustards, alkamin and alkamini; the former is a bis-half-mustard in which reactive groups are disposed at each end of the ligand, and the latter is its monofunctional analog. Alkamin is potently cytotoxic and active in experimental mouse tumor models, whereas alkamini is not. We have studied their interaction with the DNA dodecamer d(CGCGAATTCGCG)2, designated A2T2, and we provide a detailed analysis of the observed DNA-ligand adduct ions and their fragmentation products. We find that alkamini alkylates A2T2 at guanine G4 and adenines A5 and A6 in a manner consistent with covalent attack on purine N3 atoms from the minor groove of the AT tract. Alkamin also forms monofunctional adducts at G4 and both adenines in which the second mustard arm is hydrolyzed but, in addition, forms a variety of interstrand cross-links between adenines A5/A6 and A5′/A6′, an interstrand cross-link between G4 and A6′, and an intrastrand cross-link between G4 and A6. We conclude that the marked cytotoxicity of alkamin and its experimental antitumor activity could be the consequence of its ability to cross-link cellular DNA at AT tract sequences.
Technical Considerations for the Generation of Adoptively Transferred T Cells in Cancer Immunotherapy

A significant function of the immune system is the surveillance and elimination of aberrant cells that give rise to cancer. Even when tumors are well established and metastatic, immune-mediated spontaneous regressions have been documented. While there are have been various forms of immunotherapy, one of the most widely studied for almost 40 years is adoptive cellular immunotherapy, but its success has yet to be fully realized. Adoptive cell transfer (ACT) is a therapeutic modality that has intrigued physicians and researchers for its many theoretical benefits. Preclinical investigations and human trials have utilized natural killer (NK) cells, dendritic cells (DC), macrophages, T-cells or B-cells for ACT with the most intense research focused on T-cell ACT. T-cells are exquisitely specific to the target of its T-cell receptor (TCR), thus potentially reducing the amount of collateral damage and off-target effects from treatment. T-cells also possess a memory subset that may reduce the risk of recurrence of a cancer after the successful treatment of the primary disease. There are several options for the source of T-cells used in the generation of cells for ACT. Perhaps the most widely known source is T-cells generated from tumor-infiltrating lymphocytes (TILs). However, studies have also employed peripheral blood mononuclear cells (PBMCs), lymph nodes, and even induced pluripotent stem cells (IPSCs) as a source of T-cells. Several important technical considerations exist regarding benefits and limitations of each source of T-cells. Unique aspects of T-cells factor into their ability to be efficacious in ACT including the total number of cells available for ACT, the anti-tumor efficacy on a per cell basis, the repertoire of TCRs specific to tumor cells, and their ability to traffic to various organs that harbor tumor. Current research is attempting to unlock the full potential of these cells to effectively and safely treat cancer.
A three domain model of teacher and school executive career satisfaction

This paper presents a model of teacher and school executive satisfaction derived from a study involving 892 respondents in 71 government schools in Western Sydney, Australia. Factor analysis of survey items was utilised to develop an eight factor model of teacher satisfaction. The eight factors were named: school leadership, climate, decision making; merit promotion and local hiring; school infrastructure; school reputation; status and image of teachers; student achievement; workload and the impact of change; and professional self‐growth. Scores on the scales fell into three domains: “core business of teaching” factors (student achievement; professional self‐growth); school level factors (school leadership, climate, decision making; school infrastructure; school reputation); and system level/societal factors (workload and impact of change; status and image of teachers; merit promotion). Respondents were most satisfied with “core business” aspects and least satisfied with system level/societal factors, while school level factors showed the most variation, reflecting the influence of teachers’ specific and varying within‐school experiences. Leadership, communication and decision making styles were found to be important contributing factors to satisfaction with school based aspects of respondents’ roles. It is argued that within the important, school level domain, action to improve teacher satisfaction is most likely to be effective.
Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials.

The use of plants is as old as the mankind. Natural products are cheap and claimed to be safe. They are also suitable raw material for production of new synthetic agents. Rosemary (Rosmarinus officinalis Linn.) is a common household plant grown in many parts of the world. It is used for flavouring food, a beverage drink, as well as in cosmetics; in folk.medicine it is used as an antispasmodic in renal colic and dysmenorrhoea, in relieving respiratory disorders and to stimulate growth of hair. Extract of rosemary relaxes smooth muscles of trachea and intestine, and has choleretic, hepatoprotective and antitumerogenic activity. The most important constituents of rosemary are caffeic acid and its derivatives such as rosmarinic acid. These compounds have antioxidant effect. The phenolic compound, rosmarinic acid, obtains one of its phenolic rings from phenylalanine via caffeic acid and the other from tyrosine via dihydroxyphenyl-lactic acid. Relatively large-scale production of rosmarinic acid can be obtained from the cell culture of Coleus blumei Benth when supplied exogenously with phenylalanine and tyrosine. Rosmarinic acid is well absorbed from gastrointestinal tract and from the skin. It increases the production of prostaglandin E2 and reduces the production of leukotriene B4 in human polymorphonuclear leucocytes, and inhibits the complement system. It is concluded that rosemary and its constituents especially caffeic acid derivatives such as rosmarinic acid have a therapeutic potential in treatment or prevention of bronchial asthma, spasmogenic disorders, peptic ulcer, inflammatory diseases, hepatotoxicity, atherosclerosis, ischaemic heart disease, cataract, cancer and poor sperm motility.
Systematic identification and correction of annotation errors in the genetic interaction map of Saccharomyces cerevisiae

The yeast mutant collections are a fundamental tool in deciphering genomic organization and function. Over the last decade, they have been used for the systematic exploration of ∼6 000 000 double gene mutants, identifying and cataloging genetic interactions among them. Here we studied the extent to which these data are prone to neighboring gene effects (NGEs), a phenomenon by which the deletion of a gene affects the expression of adjacent genes along the genome. Analyzing ∼90,000 negative genetic interactions observed to date, we found that more than 10% of them are incorrectly annotated due to NGEs. We developed a novel algorithm, GINGER, to identify and correct erroneous interaction annotations. We validated the algorithm using a comparative analysis of interactions from Schizosaccharomyces pombe. We further showed that our predictions are significantly more concordant with diverse biological data compared to their mis-annotated counterparts. Our work uncovered about 9500 new genetic interactions in yeast.
Detection of Paroxysmal Atrial Fibrillation in Stroke/Tia Patients

One-third of stroke and transient ischemic attack (TIA) are cryptogenic, and paroxysmal atrial fibrillation (PAF) has been suggested as a possible cause for these cryptogenic strokes. Multiple studies have recently evaluated long-term cardiac rhythm monitoring with good yield for PAF. The duration of monitoring varies between studies as well as the qualifying event definition. Moreover, the clinical significance of very brief atrial fibrillation events is unclear in the literature. This paper provides an overview of current advances in the detection of paroxysmal atrial fibrillation, the clinical and genetic factors predictive of arrhythmia detection, and the therapeutic dilemma concerning this approach.
Nonsurgical healing of large periradicular lesions using a triple antibiotic paste: A case series

The infection of the root canal system is considered to be a polymicrobial infection, consisting of both aerobic and anaerobic bacteria. Because of the complexity of the root canal infection, it is unlikely that any single antibiotic could result in effective sterilization of the canal. A combination of antibiotic drugs (metronidazole, ciprofloxacin, and minocycline) is used to eliminate target bacteria, which are possible sources of endodontic lesions. Three case reports describe the nonsurgical endodontic treatment of teeth with large periradicular lesions. A triple antibiotic paste was used for 3 months. After 3 months, teeth were asymptomatic and were obturated. The follow-up radiograph of all the three cases showed progressive healing of periradicular lesions. The results of these cases show that when most commonly used medicaments fail in eliminating the symptoms then a triple antibiotic paste can be used clinically in the treatment of teeth with large periradicular lesions.
High short-term mortality in hospitalized patients with advanced dementia: lack of benefit of tube feeding.

BACKGROUND The influence of tube feeding on survival in hospitalized patients with advanced dementia is controversial. OBJECTIVE To assess long-term survival in an inception cohort, incident tube feeding placement during the index hospitalization, and the influence of tube feeding on survival in this group of patients. SUBJECTS AND METHODS Ninety-nine hospitalized patients with advanced dementia and an available surrogate decision maker were followed up through and after the index hospitalization for mortality and placement of a feeding tube. Other variables measured included advance directive status, presence of a long-term primary care physician, level of involvement of the surrogate decision maker, admitting diagnosis, prior hospitalizations, comorbidities, and diagnosis related group diagnostic category. RESULTS A new feeding tube was placed in 50% (51/99) of the study patients during the index hospitalization, 31% (31/99) left the hospital without a feeding tube, and 17% (17/99) were admitted with a feeding tube already in place. By stepwise logistic regression analysis, predictors of new feeding tube placement included African American ethnicity (odds ratio, 9.43; 95% confidence interval, 2.1-43.2) and residence in a nursing home (odds ratio, 4.9; 95% confidence interval, 1.02-2.5). Median survival of the 99 patients was 175 days. Eighty-five (85%) survived the index hospitalization, and 28 (28%) were still alive at last follow-up, a range of 1.3 to 4.2 years after enrollment in the study. Tube feeding was not associated with survival (P =.90). An admitting diagnosis of infection was associated with higher mortality (odds ratio, 1.9; 95% confidence interval, 1.01-3.6). CONCLUSIONS In this cohort of hospitalized patients with advanced dementia, risk of receiving a new feeding tube is high, associated with African American ethnicity, and prior residence in a nursing home, and has no measurable influence on survival. With or without a feeding tube, these patients have a 50% six-month median mortality.
Knowledge management, sustainable innovation, and pre‐service teacher education in Singapore

In 1997, Singapore's Ministry of Education (MOE) committed itself to an ambitious program of pedagogical reform in Singaporean schools in anticipation of the kind of institutional challenges – particularly those in increasingly globalized labor markets – that young Singaporeans were likely to face in the coming decades. Since then, the Ministry has designed and implemented a series of initiatives that, the authors suggest, will go a considerable distance to achieve its objectives. These initiatives focus on substantial changes in the system of ‘instructional governance’ in Singapore over the past decade, and efforts to change the pattern of classroom pedagogy. But while these represent a good start, the authors argue that these initiatives do not go quite far enough to close the gap between policy and practice. And while the improvement of classroom pedagogy in the long run will depend on the improvement of initial teacher education, it is also the case that, given what is known about the circumstances that optimize professional learning in both pre‐service and in‐service programs, the improvement of teacher education will depend substantially on the prior improvement of classroom pedagogy. How Singapore might escape this conundrum is the central focus of this paper.
Demodulation of Low SNR QPSK Signal Based on Chaotic Synchronization

Signal restoration utilizing chaotic synchronization can resist strong noise and it can make signal demodulation more easy in low SNR channel. In this paper, BPSK signal restoration is introduced and a new QPSK signal demodulation based on chaotic synchronization which is used to restore QPSK signal to increase SNR is put forward. The characteristic of QPSK signal in chaotic synchronization is analyzed and two path systems is brought in to deal with the complexity of QPSK signal. Then a global design of new QPSK signal demodulation is set up and finally it is proved through simulation and compared with the traditional coherent demodulation.
Endemic Foci of the Tick-Borne Relapsing Fever Spirochete Borrelia crocidurae in Mali, West Africa, and the Potential for Human Infection

Background Tick-borne relapsing fever spirochetes are maintained in endemic foci that involve a diversity of small mammals and argasid ticks in the genus Ornithodoros. Most epidemiological studies of tick-borne relapsing fever in West Africa caused by Borrelia crocidurae have been conducted in Senegal. The risk for humans to acquire relapsing fever in Mali is uncertain, as only a few human cases have been identified. Given the high incidence of malaria in Mali, and the potential to confuse the clinical diagnosis of these two diseases, we initiated studies to determine if there were endemic foci of relapsing fever spirochetes that could pose a risk for human infection. Methodology/Principal Findings We investigated 20 villages across southern Mali for the presence of relapsing fever spirochetes. Small mammals were captured, thin blood smears were examined microscopically for spirochetes, and serum samples were tested for antibodies to relapsing fever spirochetes. Ornithodoros sonrai ticks were collected and examined for spirochetal infection. In total, 11.0% of the 663 rodents and 14.3% of the 63 shrews tested were seropositive and 2.2% of the animals had active spirochete infections when captured. In the Bandiagara region, the prevalence of infection was higher with 35% of the animals seropositive and 10% infected. Here also Ornithodoros sonrai were abundant and 17.3% of 278 individual ticks tested were infected with Borrelia crocidurae. Fifteen isolates of B. crocidurae were established and characterized by multi-locus sequence typing. Conclusions/Significance The potential for human tick-borne relapsing fever exists in many areas of southern Mali.
Glucocorticoid Receptor Interaction with 14-3-3 and Raf-1, a Proposed Mechanism for Cross-talk of Two Signal Transduction Pathways*

The glucocorticoid receptor (GR) functions as a ligand-dependent transcription factor. In the present study we describe a specific immunoaffinity chromatography purification of GR from liver cytosol from adrenalectomized rats that may be used to identify hitherto unknown cytosolic GR interacting proteins. We have identified the ubiquitously expressed 14-3-3 as well as Raf-1, a downstream effector of Ras, as GR co-purifying proteins. In our semi-quantitative analysis liganded/activated GR showed the strongest interaction with 14-3-3 and Raf-1, but 14-3-3 was also found to co-purify with GR in a nonliganded/nonactivated state. By extensive salt washes we were also able to demonstrate that the glucocorticoid induced interaction between GR, 14-3-3, and Raf-1, respectively, is remarkably stable and withstood 2.4 m salt. The interaction between GR and 14-3-3 was also verified by 14-3-3 co-immunoprecipitation studies. Our observations that GR and Raf-1 are found within the same protein complex (“receptosome”) in the cytoplasm of rat liver cells could provide a mechanistic explanation for glucocorticoid effects on the Raf-1-Ras signaling pathway.
Exploring Differences and Similarities between Hierarchical Decentralized Search and Human Navigation in Information Networks

Decentralized search in networks is an activity that is often performed in online tasks. It refers to situations where a user has no global knowledge of a network’s topology, but only local knowledge. On Wikipedia for instance, humans typically have local knowledge of the links emanating from a given Wikipedia article, but no global knowledge of the entire Wikipedia graph. This makes the task of navigation to a target Wikipedia article from a given starting article an interesting problem for both humans and algorithms. As we know from previous studies, people can have very efficient decentralized search procedures that find shortest paths in many cases, using intuitions about a given network. These intuitions can be modeled as hierarchical background knowledge that people access to approximate a networks’ topology. In this paper, we explore the differences and similarities between decentralized search that utilizes hierarchical background knowledge and actual human navigation in information networks. For that purpose we perform a large scale study on the Wikipedia information network with over 500,000 users and 1,500,000 click trails. As our results reveal, simulations based on decentralized search with hierarchies created directly from the link structure of the information network are more similar to human navigation behavior than simulations based on hierarchies that are created from network-external knowledge.
Characteristics of “Masculinized” Industries

Masculinized industries encompass many work contexts described as masculine, male-dominated, and/or gendered. However, the use of any of those three latter designations hinges on the gender binary (men and women) and the inequitable distribution of power in favor of men. Describing an industry or group of industries as “masculinized” is more political, inferring the stance that male privilege applies only to certain men working in these industries. The purpose of this structured literature review was to systematically examine the literature on masculine, male-dominated, gendered, and masculinized work contexts. Findings indicated that masculinized industries create metaphorical barricades that serve to sustain long-standing traditions, customs, policies, and practices potentially detrimental to many, including gay men. Overall, this article moves the conversation about inequality in these industries beyond that of the gender binary.