Miracle-dz/UniFAR-FTUs huggingface dataset:
Association between ACE and ACTN3 genes polymorphisms and athletic performance in elite and sub-elite Chinese youth male football players

Background Previous studies have shown controversial relationships between ACE I/D and ACTN3 R577x polymorphisms and athletic performance. Therefore, the aim of this study was to assess athletic performance indicators of Chinese youth male football players with different ACE and ACTN3 gene profiles. Methods and Materials This study recruited 73 elite (26 13-year-olds, 28 14-year-olds, and 19 15-year-olds) and 69 sub-elite (37 13-year-olds, 19 14-year-olds, and 13 15-year-olds) and 107 controls (63 13-year-olds, and 44 14-year olds aged 13–15 years, all participants were of Chinese Han origin. We measured height, body mass, thigh circumference, speed, explosive power, repeat sprints ability, and aerobic endurance in elite and sub-elite players. We used single nucleotide polymorphism technology to detect controls elite and sub-elite players’ ACE and ACTN3 genotypes, Chi-squared (χ2) tests were employed to test for Hardy-Weinberg equilibrium. χ2 tests were also used to observe the association between the genotype distribution and allele frequencies between controls and elite and sub-elite players. The differences in parameters between the groups were analyzed using one-way analysis of variance and a Bonferroni’s post-hoc test, with statistical significance set at p ≤ 0.05. Results (1) The genotype distribution of the ACE I/D and ACTN3 R577x polymorphisms in controls, elite and sub-elite football players were consistent with Hardy-Weinberg equilibrium, except for the ACE genotype distribution of sub-elite players. (2) The RR and DD genotypes were significantly different between elite and sub-elite players (p = 0.024 and p = 0.02, respectively). (3) Elite players were more likely to have the RR genotype and less likely to have the DD genotype compared with sub-elite players. (4) Both elite and sub-elite RR players’ Yo-yo intermittent recovery level 1 (YYIR1) running distance was significantly longer than that of RX players (p = 0.05 and p = 0.025, respectively). However, there was no significantly different in YYIR1 running distance between elite and sub-elite RR players. (5) Elite XX players’ VO2 max was significantly higher than that of RX and sub-elite players. Conclusion These results indicate that ACE I/D and ACTN3 R577x polymorphisms are not associated with muscle power in Chinese elite and sub-elite players. The XX genotype of ACTN3 is associated with the aerobic endurance of elite players.
Screening of a Thraustochytrid Strain Collection for Carotenoid and Squalene Production Characterized by Cluster Analysis, Comparison of 18S rRNA Gene Sequences, Growth Behavior, and Morphology

Carotenoids and squalene are important terpenes that are applied in a wide range of products in foods and cosmetics. Thraustochytrids might be used as alternative production organisms to improve production processes, but the taxon is rarely studied. A screening of 62 strains of thraustochytrids sensu lato for their potential to produce carotenoids and squalene was performed. A phylogenetic tree was built based on 18S rRNA gene sequences for taxonomic classification, revealing eight different clades of thraustochytrids. Design of experiments (DoE) and growth models identified high amounts of glucose (up to 60 g/L) and yeast extract (up to 15 g/L) as important factors for most of the strains. Squalene and carotenoid production was studied by UHPLC-PDA-MS measurements. Cluster analysis of the carotenoid composition partially mirrored the phylogenetic results, indicating a possible use for chemotaxonomy. Strains in five clades produced carotenoids. Squalene was found in all analyzed strains. Carotenoid and squalene synthesis was dependent on the strain, medium composition and solidity. Strains related to Thraustochytrium aureum and Thraustochytriidae sp. are promising candidates for carotenoid synthesis. Strains closely related to Schizochytrium aggregatum might be suitable for squalene production. Thraustochytrium striatum might be a good compromise for the production of both molecule groups.
AN ANALYSIS OF MULTIPLICATIVE REGULARIZATION

Inverse problems arise in many branches of science and engineering. In order to get a good approximation of the solution of this kind of problems, the use of regularization methods is required. Tikhonov regularization is one of the most popular methods for estimating the solutions of inverse problems. This method needs a regularization parameter and the quality of the approximate solution depends on how good the regularization parameter is. The L-curve method is a convenient parameter choice strategy for selecting the Tikhonov regularization parameter and it works well most of the time. There are some problems in which the L-curve criterion does not perform properly. Multiplicative regularization is a method for solving inverse problems and does not require any parameter selection strategies. However, it turns out that there is a close connection between multiplicative regularization and Tikhonov regularization; in fact, multiplicative regularization can be regarded as defining a parameter choice rule for Tikhonov regularization. In this work, we have analyzed multiplicative regularization for finite-dimensional
Diagnostic Utility of Serum Leucine-Rich α-2-Glycoprotein 1 for Acute Appendicitis in Children

Purpose: The aim of this study is to assess the diagnostic utility of serum leucine-rich α-2-glycoprotein 1 (LRG1) in pediatric patients with acute abdominal pain, admitted to the emergency surgical unit, in order to make a prompt and accurate diagnosis of acute appendicitis. Patients and methods: Pediatric patients older than 5 years of age who presented to the emergency department from 15 October 2021 to 30 June 2022 with acute abdominal pain and suspected acute appendicitis were prospectively recruited in the study. Demographic and clinical data, as well as operative and postoperative data, were recorded. A total of 92 patients were equally distributed into two groups: children with acute appendicitis who underwent laparoscopic appendectomy and non-appendicitis patients, presenting with non-specific abdominal pain. LRG1 levels were determined using a commercially available LRG1 enzyme-linked immunosorbent assay (ELISA) kit. Serum LRG1 levels, as well as other inflammatory markers (white blood cell count (WBC), C-reactive protein (CRP) and absolute neutrophil count) were compared between groups. Results: The median level of LRG1 in serum was significantly higher in the group of children with pathohistologically confirmed acute appendicitis than in the control group, at 350.3 µg/mL (interquartile range (IQR) 165.2–560.3) and 25.7 µg/mL (IQR 14.7–36.8) (p < 0.001), respectively. Receiver operating characteristic area under the curve for LRG1 from serum was 1.0 (95% CI 0.96–1.00; p < 0.001) and the value of >69.1 µg/mL was found to perfectly separate acute appendicitis cases from controls. Additionally, as expected, each of the examined laboratory inflammatory markers provided a significantly higher values in the acute appendicitis group compared to the control group: WBC 14.6 × 109/L (IQR 12.7, 18.7) vs. 7.0 × 109/L (IQR 5.4, 9.0) (p < 0.001), CRP 16.3 mg/dL (IQR 6.9, 50.4) vs. 2.2 mg/dL (IQR 2, 2) (p < 0.001) and absolute neutrophil count 84.6% (IQR 79.5, 89.0) vs. 59.5% (IQR 51.5, 68.6) (p < 0.001). Conclusions: LRG1 in the serum was found to be a promising novel biomarker, with excellent differentiation of acute appendicitis from non-appendicitis cases in children presenting with non-specific abdominal pain.
Recent Advances in Nanoparticle-Based Optical Sensors for Detection of Pesticide Residues in Soil

The excessive and unreasonable use of pesticides has adversely affected the environment and human health. The soil, one of the most critical natural resources supporting human survival and development, accumulates large amounts of pesticide residues. Compared to traditional spectrophotometry analytical methods, nanoparticle-based sensors stand out for their simplicity of operation as well as their high sensitivity and low detection limits. In this review, we focus primarily on the functions that various nanoparticles have and how they can be used to detect various pesticide residues in soil. A detailed discussion was conducted on the properties of nanoparticles, including their color changeability, Raman enhancement, fluorescence enhancement and quenching, and catalysis. We have also systematically reviewed the methodology for detecting insecticides, herbicides, and fungicides in soil by using nanoparticles.
The new V12 ultra-small-angle neutron scattering and tomography instrument at the Hahn–Meitner Institut

The new V12 instrument at the Hahn–Meitner Institute in Berlin is a multiple setup combining several techniques to investigate the internal structure of bulk samples. It consists of two double-crystal diffractometers (DCDs) and an attenuation tomography device operating with monochromatic neutrons. The three instrument parts can be used independently at the same time. The DCDs are mainly used in the ultra-small-angle neutron scattering (USANS) regime, where they overlap the accessible range of small-angle neutron scattering instruments, while tomographic methods collect real-space information on a macroscopic scale. Together they enable structural investigations over six orders of magnitude (50 nm to 5 cm). Scattering and tomographic methods can even be combined by means of diffraction and scattering-enhanced imaging. The sample environment can be varied over a large range of temperatures and pressures for USANS measurements and a polarized USANS option is available.
Association between behavioral and psychological symptoms and cerebral small vessel disease MRI findings in memory clinic patients

Objectives Cerebral small vessel disease (SVD) is commonly observed among elderly individuals with cognitive impairment and has been recognized as a vascular contributor to dementia and behavioral and psychological symptoms (BPS), however, the relationship between BPS and SVD burden remains unclear. Methods We prospectively recruited 42 patients with mild cognitive impairment (MCI) or mild dementia from the memory clinic in our hospital, who were assigned to either a clinical dementia rating (CDR) of 0.5 or 1.0, respectively. The presence of BPS was determined through interviews with caregivers. The patients underwent brain MRI and three types of SVD scores, total, cerebral amyloid angiopathy (CAA), and modified CAA, were assigned. Patients were also evaluated through various neuropsychological assessments. Results The CDR was significantly higher in patients with BPS (p = 0.001). The use of antihypertensive agents was significantly higher in patients without BPS (p = 0.038). The time taken to complete trail making test set-A was also significantly longer in patients with BPS (p = 0.037). There was no significant difference in total SVD and CAA-SVD score (p = 0.745, and 0.096) and the modified CAA-SVD score was significantly higher in patients with BPS (p = 0.046). In addition, the number of total CMBs and lobar CMBs was significantly higher in patients with BPS (p = 0.001 and 0.001). Receiver operating characteristic curves for BPS showed that for modified CAA-SVD, a cutoff score of 3.5 showed 46.7% sensitivity and 81.5% specificity. Meanwhile, for the total number of cerebral microbleeds (CMBs), a cut-off score of 2.5 showed 80.0% sensitivity and 77.8% specificity and for the number of lobar CMBs, a cut-off score of 2.5 showed 73.3% sensitivity and 77.8% specificity. Conclusion Overall, patients with BPS showed worse CDRs, reduced psychomotor speed, higher modified CAA-SVD scores, larger numbers of total and lobar CMBs. We propose that severe modified CAA scores and higher numbers of total and lobar CMBs are potential risk factors for BPS in patients with mild dementia or MCI. Therefore, by preventing these MRI lesions, the risk of BPS may be mitigated.
Generation of a Perfusable 3D Lung Cancer Model by Digital Light Processing

Lung cancer still has one of the highest morbidity and mortality rates among all types of cancer. Its incidence continues to increase, especially in developing countries. Although the medical field has witnessed the development of targeted therapies, new treatment options need to be developed urgently. For the discovery of new drugs, human cancer models are required to study drug efficiency in a relevant setting. Here, we report the generation of a non-small cell lung cancer model with a perfusion system. The bioprinted model was produced by digital light processing (DLP). This technique has the advantage of including simulated human blood vessels, and its simple assembly and maintenance allow for easy testing of drug candidates. In a proof-of-concept study, we applied gemcitabine and determined the IC50 values in the 3D models and 2D monolayer cultures and compared the response of the model under static and dynamic cultivation by perfusion. As the drug must penetrate the hydrogel to reach the cells, the IC50 value was three orders of magnitude higher for bioprinted constructs than for 2D cell cultures. Compared to static cultivation, the viability of cells in the bioprinted 3D model was significantly increased by approximately 60% in the perfusion system. Dynamic cultivation also enhanced the cytotoxicity of the tested drug, and the drug-mediated apoptosis was increased with a fourfold higher fraction of cells with a signal for the apoptosis marker caspase-3 and a sixfold higher fraction of cells positive for PARP-1. Altogether, this easily reproducible cancer model can be used for initial testing of the cytotoxicity of new anticancer substances. For subsequent in-depth characterization of candidate drugs, further improvements will be necessary, such as the generation of a multi-cell type lung cancer model and the lining of vascular structures with endothelial cells.
Preserving Soybean Oil for the Frying of Breaded Butterfly Shrimp Using Natural Rosemary Antioxidant

Fried foods and frying oil are subjects that warrant the attention of researchers because of their high consumption. Indeed, frying conditions make these oils very sensitive to lipid oxidation which deteriorates the quality and nutritional properties of the food. In this study, we examined the effect of rosemary extract (ROE), known for its high antioxidant activity, in soybean oil used to fry breaded butterfly shrimp, by measuring the induction period with OXIPRES, total polar material (TPM), peroxide index (PI), and free fatty acids (FFA). This evaluation was performed in comparison with control oils without antioxidants. The results showed a significant difference between the oils according to the analyzed parameters, especially in the final hours of frying. The treatment of the oil with rosemary extract effectively delayed its oxidation, having lower levels in all the oxidation markers that were analyzed. It was also found that rosemary extract is able to reduce oil consumption by fried foods. Therefore, ROE ensures soybean oil a high stability against oxidation and a longer shelf life, making it a good natural alternative to synthetic antioxidants.
Thermal resistance of heterostructure lasers

A GaAs−GaAlAs heterostructure laser is modeled as a stripe heat source embedded in a layered structure, and an analytic expression is given for the steady−state thermal resistance 〈R〉 of the model. Over the range of typical layer thicknesses and conductivities, and for heat generated uniformly in the active region, 〈R〉 varies between 14 and 31 K/W for a 12×375−μ active region. Four types of heat sinks are shown to contribute an additional 3 to 10 K/W. Design implications are drawn for various properties including layer thicknesses, heat−sink and bond parameters, and radiative heat transfer by spontaneous emission. In disagreement with the common tacit assumption of a unique active−region temperature, it is found that about 40% of the temperature drop within the laser occurs in the active region (center to edge).
Doppler Tomographic Observations of Kepler-13b

Abstract We present Doppler tomographic observations of the transiting planet Kepler-13b (aka KOI-13b), a highly inflated hot Jupiter orbiting the Teff=8500 K primary of a hierarchical triple stellar system. As the planet transits the rapidly rotating host star, it successively blocks regions of the stellar disk with different radial velocities, causing a “bump” in the stellar spectral line shape, which we resolve spectroscopically. The manner in which this perturbation moves across the stellar line during the transit gives information on the relative alignment between the stellar spin and planetary orbital angular momentum vectors. This is a powerful statistical probe of planetary migration processes, as the expected spin-orbit misalignment distributions from dynamically cold migration (disk interactions) and dynamically hot migration (planet-planet scattering, Kozai cycles) are significantly different: the former will lead to primarily aligned orbits; the latter to a wide distribution. Doppler tomography also promises to be a powerful technique for confirming transiting planet candidates around rapidly rotating stars like Kepler-13A, which are not amenable to radial velocity follow-up and thus are currently a poorly sampled region of parameter space.
An equivalence between two algorithms for a class of quadratic programming problems with mmatrices

In this paper the behaviour of the gradient projection method for the Quadratic Programming problems with M-Matrices and lower bounds on variables is analysed. It is shown that the Chandbasekaran method for solving such problems is simply a realization of the Newton projection method if for the latter one the starting point has components equal to lower bounds on variables.
A literature review of bench top and pilot lead corrosion assessment studies

Bench top and pilot lead corrosion studies are gaining more interest, considering revisions and upcoming improvements to the Lead and Copper Rule. This literature review identified studies ranging from simpler month(s)‐long bench top dump‐and‐fill stagnant water tests (coupon tests/standing pipe tests) to more complicated year(s)‐long intermittent flow pilot studies (recirculating pipe loops/once through pipe rigs). With increasing complexity in design and operation, studies more closely approximated real plumbing conditions (e.g., by incorporating harvested lead pipes and intermittent flow regimes) at increased cost, footprint, and duration. Comparison of bench top and pilot designs (in terms of lead test piece age/dimensions/configuration/replicates, study duration, sample collection, and other factors) can assist drinking water utilities, consultants, academics, and others to select a design that matches their needs and constraints. No matter the choice, surrogate systems cannot replace actual system water testing and are best complemented by other corrosion assessment tools.
Rapamycin Perfluorocarbon Nanoparticle Mitigates Cisplatin-Induced Acute Kidney Injury

For nearly five decades, cisplatin has played an important role as a standard chemotherapeutic agent and been prescribed to 10–20% of all cancer patients. Although nephrotoxicity associated with platinum-based agents is well recognized, treatment of cisplatin-induced acute kidney injury is mainly supportive and no specific mechanism-based prophylactic approach is available to date. Here, we postulated that systemically delivered rapamycin perfluorocarbon nanoparticles (PFC NP) could reach the injured kidneys at sufficient and sustained concentrations to mitigate cisplatin-induced acute kidney injury and preserve renal function. Using fluorescence microscopic imaging and fluorine magnetic resonance imaging/spectroscopy, we illustrated that rapamycin-loaded PFC NP permeated and were retained in injured kidneys. Histologic evaluation and blood urea nitrogen (BUN) confirmed that renal structure and function were preserved 48 h after cisplatin injury. Similarly, weight loss was slowed down. Using western blotting and immunofluorescence staining, mechanistic studies revealed that rapamycin PFC NP significantly enhanced autophagy in the kidney, reduced the expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1), as well as decreased the expression of the apoptotic protein Bax, all of which contributed to the suppression of apoptosis that was confirmed with TUNEL staining. In summary, the delivery of an approved agent such as rapamycin in a PFC NP format enhances local delivery and offers a novel mechanism-based prophylactic therapy for cisplatin-induced acute kidney injury.
Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

There are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichment. Effective enrichment procedures are required to meet the requirements for food detection. Here, boronate-functionalized cellulose filter paper and specific fluorescent probes were combined. An integrated approach for the enrichment of detection of Staphylococcus aureus was proposed. The modification of polyethyleneimine demonstrated a significant effect in enhancing the bacterial enrichment, and the boronate affinity efficiency of the paper was increased by about 51~132%. With optimized conditions, the adsorption efficiency for S. aureus was evaluated as 1.87 × 108 CFU/cm2, the linear range of the fluorescent analysis was 104 CFU/mL~108 CFU/mL (R2 = 0.9835), and the lowest limit of detection (LOD) was calculated as 2.24 × 102 CFU/mL. Such efficiency was validated with milk and yogurt samples. These results indicated that the material had a high enrichment capacity, simple operation, and high substrate tolerance, which had the promising potential to be the established method for the fast detection of food pathogens.
Communication style drives emergent leadership attribution in virtual teams

Leader selection plays a key role in how human social groups are formed and maintained. Leadership is either assigned through formal processes within an organization, or emerges informally through interactions with other group members–particularly in novel contexts. COVID-19 has accelerated the adoption of virtual meetings and more flexible team structures. However our understanding of how assigned leadership influences subsequent leadership emergence in virtual settings is limited. Here we examine the relationship between assigned leadership within an existing organization and subsequent emergent leadership attributions as members engage in virtual interactions. To do so, we created and implemented a novel virtual group decision-making task designed to support quantification of a more comprehensive set of communication style elements, such as speech dynamics and facial expressions, as well as task behaviors. Sixteen members of a real world organization engaged four repeated rounds of a group decision making task with new team members each time. We found participants made novel attributions of emergent leadership rather than relying solely on existing assigned leadership. While assigned leadership did influence leadership attributions, communication style, including amount of speech but also variability in facial expressions, played a larger role. The behavior of these novel emergent leaders was also more consistent with expectations of leadership behavior: they spoke earlier, more often, and focused more on the correct decision than did assigned leaders. These findings suggest that, even within existing social networks, virtual contexts promote flexible group structures that depend more on communication style and task performance than assigned leadership.