Miracle-dz/UniFAR-FTUs huggingface dataset:
In-Silico Screening of Mitragynine Derivates from the Genus Mitragyna Korth Targeting the Main Protease of the SARS-COV-2

Coronavirus Diseases 2019, caused by SARS-CoV-2, has been a significant threat to global public health. Unfortunately, effective COVID-19 vaccines and clinically-proven anti-SARS-CoV-2 drugs remain unavailable. This study was carried out aiming to predict the potential effect of mitragynine derivates from the Genus Mitragyna Korth as an inhibitor of Mpro, the main protease of the SARS-CoV-2, by in silico molecular docking study. The crystal structure of the main protease of SARS-CoV-2 as an active site target was obtained from the PDB database (rcsb.org) with PDB ID: 5R84 and 6LU7 with the native ligand of Z31792168 and N3, respectively. The analysis of in silico molecular docking was conducted using Autodock 4.2. 6 (100 docking runs). The central grid was placed on HIS41 and CYS145 with a grid box comprised of 40x30x34 (for protein 5R84) and 36x62x40 (for protein 6LU7) points spaced by 0.375 Å was centered on the active site of X=9,812; Y=-0,257; Z=20,849 and X=-9.732; Y=11.403; X=68,483 (XYZ-coordinates), respectively. Our research indicated that mitrjavine and ajmalicine exhibit greater potential inhibition of the active site on the Mpro of SARS-CoV-2, even stronger than native ligands. We believed that these compounds are promising candidates to be examined in further COVID-19 drug discovery studies.
Effects of Higher Sloshing Modes on the Response of Rectangular Concrete Water Storage Tanks with Different Aspect Ratios to Near-Field Earthquakes

Near-field earthquakes have been shown to have different effects on structures than far-field events. This study examines the dynamic response of a rectangular concrete liquid storage tank with tapered walls to near-field ground motions, with particular emphasis on the effect of higher sloshing modes. The tank’s numerical modeling, calibrated using experimental results, was performed considering the tank’s wall flexibility. Seven selected near-field records were applied in each case, and the effects of the first five sloshing modes on the tank response at three different locations, including the corner, middle of the long wall, and middle of the short wall, were investigated. The effect of the earthquake incident angle on the tank’s response was also studied by applying major and minor horizontal earthquake components once along the longer and shorter tank walls, respectively, and vice versa. Results show that the tank corner may have a sloshing height up to 50% greater than the middle of the walls and that the maximum sloshing response is substantially influenced by the spectral acceleration value at the first sloshing period. Higher sloshing modes are found to affect the sloshing response, with a maximum R2 score of 0.95, depending on the excitation’s incidence angle.
Multi-Objective Bayesian Optimization Design of Elliptical Double Serpentine Nozzle

The use of traditional optimization methods in engineering design problems, specifically in aerodynamic and infrared stealth optimization for engine nozzles, requires a large number of objective function evaluations, therefore introducing a considerable challenge in terms of time constraints. In this paper, this limitation is addressed by using a sample-efficient multi-objective Bayesian optimization that takes Kriging as a surrogate model and Expected Hypervolume Improvement as the infill criterion. Using this approach, the probabilistic model is continuously established and updated, and the approximate Pareto front is obtained at a relatively small computational budget. The objective of this work is to evaluate the applicability of employing a multi-objective Bayesian optimization framework for the aerodynamic-infrared shape optimization of an elliptical double serpentine nozzle at 6 km flight condition, where the objective functions are evaluated by means of high-fidelity computational fluid dynamics and reversed Monte Carlo ray tracing simulations. We achieve good results in both infrared radiation signature reduction and aerodynamic performance improvement with a reasonable number of evaluations, indicating that the proposed method is effective and efficient for tackling the computationally intensive optimization challenges in the aircraft design.
Application of economic instruments, tradable licences and good governance for sustainable irrigation water conservation in South Africa.

According to the National Water Policy in South Africa, the objectives are to achieve equitable access to water, and to promote effi cient and sustainable use for optimum social and economic benefi t. The National Water Act provides the legal framework, and the National Water Resource Strategy explains the ways in which water resources will be managed. Several interrelated actions are taken to implement water conservation and demand management. These include levying of charges for water resource management, water resource development, water use and waste discharge; regulations to encourage and enforce water measurement; adoption of administrative and market processes for the allocation of water use entitlements and decentralization of authority for water management to catchment management agencies (CMAs). The results of completed and ongoing research projects in South Africa demonstrate the application of technologies for direct and indirect water measurement in rivers, canals and pipelines. Decision support systems are available to determine the cost of irrigation and assess the risks of agricultural water management on farms. Empirical analyses have been done for temporary or permanent transfers and lease or trade of water use entitlements on irrigation schemes with stable and variable water supply. The establishment and functioning of CMAs for local water management by different water use sectors including irrigation have been evaluated. On the basis of these studies, it is concluded that volumetric charging and cost recovery of water supply services through a two-part charging system are practically feasible. Complete description of water use entitlements and provision of information must improve to increase security and reduce the risks associated with market trades. Training and capacity building are essential to support participation by all water users and maintain standards of accountability and effective governance for irrigation management.
Piezo1 Regulation Involves Lipid Domains and the Cytoskeleton and Is Favored by the Stomatocyte–Discocyte–Echinocyte Transformation

Piezo1 is a mechanosensitive ion channel required for various biological processes, but its regulation remains poorly understood. Here, we used erythrocytes to address this question since they display Piezo1 clusters, a strong and dynamic cytoskeleton and three types of submicrometric lipid domains, respectively enriched in cholesterol, GM1 ganglioside/cholesterol and sphingomyelin/cholesterol. We revealed that Piezo1 clusters were present in both the rim and the dimple erythrocyte regions. Upon Piezo1 chemical activation by Yoda1, the Piezo1 cluster proportion mainly increased in the dimple area. This increase was accompanied by Ca2+ influx and a rise in echinocytes, in GM1/cholesterol-enriched domains in the dimple and in cholesterol-enriched domains in the rim. Conversely, the effects of Piezo1 activation were abrogated upon membrane cholesterol depletion. Furthermore, upon Piezo1-independent Ca2+ influx, the above changes were not observed. In healthy donors with a high echinocyte proportion, Ca2+ influx, lipid domains and Piezo1 fluorescence were high even at resting state, whereas the cytoskeleton membrane occupancy was lower. Accordingly, upon decreases in cytoskeleton membrane occupancy and stiffness in erythrocytes from patients with hereditary spherocytosis, Piezo1 fluorescence was increased. Altogether, we showed that Piezo1 was differentially controlled by lipid domains and the cytoskeleton and was favored by the stomatocyte–discocyte–echinocyte transformation.
Video Project to Enhance Comprehension of TOEIC Material

This study aims to investigate the application of video project to comprehend TOEIC materials in the "TOEIC preparation" course. The research method used is mix method, which is descriptive qualitative and quantitative research. During one semester, students are given the task of creating video content that re-explains each material that has been explained by the lecturer in the class. Data was collected through observation, interviews, questionnaires to gain an in-depth comprehension of students' experiences and perceptions regarding the assignment of video projects, and pre-test and post-test to know the enhancing of comprehension. The score’s TOEIC of pre-test and post-test were analyzed using the T-test. The research findings show that the assignment of video projects has a positive impact on students' comprehension of the TOEIC preparation material. Through the process of making videos, students are actively involved in re-interpreting and conveying information in a structured manner. Students' English speaking ability also experienced a significant increase. In producing video content, they practice speaking English by expressing ideas and explaining material verbally. In addition, the assignment of video projects also increases student motivation and involvement in learning. This creative activity provides an interesting and interactive way to study TOEIC preparation material, thereby increasing their interest and participation in the learning process. The videos produced by students can also be an additional learning resource for other students in comprehension the TOEIC preparation material. This study concludes that the use of video project is effective in increasing comprehension of the material in the TOEIC preparation course. The result questionnaire revealed that 60% strongly agree and 30% agree. The implication of this research is the importance of adopting active, creative and collaborative learning strategies in an effort to improve the quality of learning English. Based on the quantitative result using T test analysis, it could be said that video project could enhance TOEIC comprehension.
Relationship between Health Literacy and Proper Antibiotic Use Awareness in Tehran 2019-2020

Background Improper use of antibiotics is one of health care problems that can lead to side effects or antibiotic resistance without benefit. This study aimed to evaluate the association between health literacy and knowledge on appropriate use of antibiotics in a population sample from Tehran. Methods This was a cross-sectional, descriptive-analytic study on adults aged between 18 and 65 years. Health literacy was measured by the Health literacy for Iranian Adults (HELIA: Health Literacy for Iranian Adults) questionnaire, and awareness about proper antibiotic use was evaluated by a checklist designed based on a literature review and expert’s opinion in domains of knowledge and attitude. Both an online Google Forms questionnaire and a paper questionnaire completed by outpatients from particular clinics in Tehran's north, west, or center were used to collect the data. SPSS Version 22 was used to analyze the data. Results Out of 359 participants, 59.6% were women, and 66.8% had a university education level. Internet and health care workers were the main sources of health information. The mean score of health literacy was 71.4 out of 100, and 67.4% of the respondents had excellent or sufficient health literacy. The mean antibiotic awareness score was 10.5 out of 13, and the score in the domain of attitude was higher than knowledge. There was a significant relationship between health literacy and awareness about proper antibiotic use (P < 0.001). Health literacy was significantly higher in women (P = 0.001), people with higher education levels (P = 0.001), and financial sufficiency (P = 0.0038). Also, there was a significant relationship between awareness about proper antibiotic usage and age (P = 0.007) and financial sufficiency (P < 0.001) of the respondents. The online questionnaire users were not different in terms of their health literacy, but they were more educated and aware of antibiotics. Conclusion The level of health literacy and awareness about the proper use of antibiotics in this study was good. level of health literacy was closely related to knowledge about the proper use of antibiotics, thus, it seems that promoting health literacy may increase awareness about proper antibiotic use.
The Tragic Revelation of the Transgender in “The Man Who Thought Himself a Woman” (Anonymous)

The aim of this article is to analyze the tragic revelation of the transgender in an anonymous short story entitled “The Man Who Thought Himself a Woman. ” The analysis is supported by the concepts of transgender and cross-dressing since the male main character who has a wife and two daughters gradually becomes a transgender preceded by becoming a secret cross-dresser. The library research method is used to collect the relevant data while the qualitative research method is used in the general analysis. The literary analysis is supported by textual research method focusing on character, conflict, and setting, and contextual research method focusing on the concepts of transgender and cross-dressing. The result shows that becoming transgender is a difficult process and needs much bravery. Since the main male character feels unable to face the consequence as a transgender, he starts by becoming a cross-dresser in secret at night for a couple of hours at the house basement. When he finally wants to declare in public about his coming out in a full female clothes, he prefers to present it as his last performance in his well-arranged death.
Molecular Modulators and Receptors of Selective Autophagy: Disease Implication and Identification Strategies

Autophagy is a highly conserved physiological process that maintains cellular homeostasis by recycling cellular contents. Selective autophagy is based on the specificity of cargo recognition and has been implicated in various human diseases, including neurodegenerative diseases and cancer. Selective autophagy receptors and modulators play key roles in this process. Identifying these receptors and modulators and their roles is critical for understanding the machinery and physiological function of selective autophagy and providing therapeutic value for diseases. Using modern researching tools and novel screening technologies, an increasing number of selective autophagy receptors and modulators have been identified. A variety of Strategies and approaches, including protein-protein interactions (PPIs)-based identification and genome-wide screening, have been used to identify selective autophagy receptors and modulators. Understanding the strengths and challenges of these approaches not only promotes the discovery of even more such receptors and modulators but also provides a useful reference for the identification of regulatory proteins or genes involved in other cellular mechanisms. In this review, we summarize the functions, disease association, and identification strategies of selective autophagy receptors and modulators.
Patients with Parkinson's disease demonstrate deficits in visual‐spatial memory in the Chinese Visual Retention Test

Abstract Objective We aimed to explore the existence of visual‐spatial memory deficit in patients with Parkinson's disease (PD) without dementia in the Chinese Visual Retention Test, as well as to assess whether their performance is related to age, duration, severity, stage, and dopamine (DA) dose. Methods Forty‐two patients with PD and 30 healthy controls were included in our study. The Chinese Visual Retention Test was used to evaluate the visual‐spatial memory of the subjects. Parameters of the Chinese Visual Retention Test were compared between the two groups. Correlation analysis and multiple linear regression analysis were used to explore the associations of the Chinese Visual Retention Test with age, duration, severity, stage of PD, and DA dose. Results Three correct scores in the Chinese Visual Retention Test were all significantly lower in the PD group than in the control group. The total error scores, error scores of omissions, deformation, and persistence in the PD group were significantly higher than those in the control group. Correlation analysis showed the total error scores in the Chinese Visual Retention Test was positively correlated with UPDRS III score and H‐Y classification. Multiple linear regression analysis showed that the total error scores in the Chinese Visual Retention Test were associated with the UPDRS III score and H‐Y classification. Conclusion Patients with PD without dementia had visual‐spatial memory deficits in the Chinese Visual Retention Test which may be affected by the severity and clinical stage of PD.
Biology-driven therapy advances in high-grade serous ovarian cancer

Following a period of slow progress, the completion of genome sequencing and the paradigm shift relative to the cell of origin for high grade serous ovarian cancer (HGSOC) led to a new perspective on the biology and therapeutic solutions for this deadly cancer. Experimental models were revisited to address old questions, and improved tools were generated. Additional pathways emerging as drivers of ovarian tumorigenesis and key dependencies for therapeutic targeting, in particular, VEGF-driven angiogenesis and homologous recombination deficiency, were discovered. Molecular profiling of histological subtypes of ovarian cancer defined distinct genetic events for each entity, enabling the first attempts toward personalized treatment. Armed with this knowledge, HGSOC treatment was revised to include new agents. Among them, PARP inhibitors (PARPis) were shown to induce unprecedented improvement in clinical benefit for selected subsets of patients. Research on mechanisms of resistance to PARPis is beginning to discover vulnerabilities and point to new treatment possibilities. This Review highlights these advances, the remaining challenges, and unsolved problems in the field.
Exosome‐delivered miR‐410‐3p reverses epithelial‐mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion

Abstract Current studies have indicated that insufficient trophoblast epithelial‐mesenchymal transition (EMT), migration and invasion are crucial for spontaneous abortion (SA) occurrence and development. Exosomal miRNAs play significant roles in embryonic development and cellular communication. Hereon, we explored the roles of serum exosomes derived from SA patients on trophoblast EMT, migration and invasion. Exosomes were isolated from normal control (NC) patients with abortion for unplanned pregnancy and SA patients, then characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting. Exosomal miRNA profiles were identified by miRNA sequencing. The effects of serum exosomes on trophoblast migration and invasion were detected by scratch wound healing and transwell assays, and other potential mechanisms were revealed by quantitative real‐time PCR (RT‐PCR), western blotting and dual‐luciferase reporter assay. Finally, animal experiments were used to explore the effects of exosomal miR‐410‐3p on embryo absorption in mice. The serum exosomes from SA patients inhibited trophoblast EMT and reduced their migration and invasion ability in vitro. The miRNA sequencing showed that miR‐410‐3p was upregulated in SA serum exosomes. The functional experiments showed that SA serum exosomes restrained trophoblast EMT, migration and invasion by releasing miR‐410‐3p. Mechanistically, SA serum exosomal miR‐410‐3p inhibited trophoblast cell EMT, migration and invasion by targeting TNF receptor‐associated factor 6 (TRAF6) at the post‐transcriptional level. Besides, SA serum exosomal miR‐410‐3p inhibited the p38 MAPK signalling pathway by targeting TRAF6 in trophoblasts. Moreover, milk exosomes loaded with miR‐410‐3p mimic reached the maternal‐fetal interface and aggravated embryo absorption in female mice. Clinically, miR‐410‐3p and TRAF6 expression were abnormal and negatively correlated in the placental villi of SA patients. Our findings indicated that exosome‐derived miR‐410‐3p plays an important role between SA serum and trophoblasts in intercellular communication, suggesting a novel mechanism by which serum exosomal miRNA regulates trophoblasts in SA patients.
DNA damage response-related immune activation signature predicts the response to immune checkpoint inhibitors: from gastrointestinal cancer analysis to pan-cancer validation

Objective: DNA damage response (DDR) deficiency has emerged as a prominent determinant of tumor immunogenicity. This study aimed to construct a DDR-related immune activation (DRIA) signature and evaluate the predictive accuracy of the DRIA signature for response to immune checkpoint inhibitor (ICI) therapy in gastrointestinal (GI) cancer. Methods: A DRIA signature was established based on two previously reported DNA damage immune response assays. Clinical and gene expression data from two published GI cancer cohorts were used to assess and validate the association between the DRIA score and response to ICI therapy. The predictive accuracy of the DRIA score was validated based on one ICI-treated melanoma and three pan-cancer published cohorts. Results: The DRIA signature includes three genes (CXCL10, IDO1, and IFI44L). In the discovery cancer cohort, DRIA-high patients with gastric cancer achieved a higher response rate to ICI therapy than DRIA-low patients (81.8% vs. 8.8%; P < 0.001), and the predictive accuracy of the DRIA score [area under the receiver operating characteristic curve (AUC) = 0.845] was superior to the predictive accuracy of PD-L1 expression, tumor mutational burden, microsatellite instability, and Epstein–Barr virus status. The validation cohort demonstrated that the DRIA score identified responders with microsatellite-stable colorectal and pancreatic adenocarcinoma who received dual PD-1 and CTLA-4 blockade with radiation therapy. Furthermore, the predictive performance of the DRIA score was shown to be robust through an extended validation in melanoma, urothelial cancer, and pan-cancer. Conclusions: The DRIA signature has superior and robust predictive accuracy for the efficacy of ICI therapy in GI cancer and pan-cancer, indicating that the DRIA signature may serve as a powerful biomarker for guiding ICI therapy decisions.
Degree-days and off-host longevity of cattle fever ticks, Boophilus spp. (Acari: Ixodidae) in south Texas pastures

Abstract Cattle fever ticks, Boophilus microplus (Canestrini) and Boophilus annulatus (Say), are native to Eurasia and have invaded the New World as vectors of bovine Babesiosis. Due to severe losses in livestock production, an eradication program by the USDA was established. Premises infested with Boophilus ticks are subject to regulatory oversight that includes acaricide treatment and quarantine. The quarantine duration varies between summer through winter and is based on the available information on the persistence of the off-host stages in pastures far from south Texas. The objective of this study was to assess the specific effect of thermal stress measured in degree-days on the longevity of B. annulatus and B. microplus off-host stages in south Texas pastures. Our results demonstrate that the longest off-host persistence for B. annulatus was 142 days under canopied habitat in the winter and 130 days in the summer. On the other hand, B. microplus off-host persistence was 113 days under canopy and 103 days in exposed habitat, both in winter. The results indicate that temperature was a key variable that prolonged the survival of B. annulatus but not B. microplus. In areas such as south Texas at the northernmost extent of B. microplus range, where mild winters prevail, we found a maximum persistence of less than 4 months (113 days), very close to the previous published record of 116 days. A reduction in the Texas pasture quarantine period from 9 months to 6 months would be justified for this species.