Miracle-dz/UniFAR-FTUs huggingface dataset:
Coaching in an Acute Pediatric Setting: A Qualitative Approach to Understanding the Perspectives of Occupational Therapists

Abstract Aims To identify barriers and enablers to implementing coaching in acute pediatric settings from the perspective of occupational therapists and develop an implementation plan to address the identified barriers at a large metropolitan hospital. Methods Participatory Action Research was used, and two stages of focus groups were conducted with 17 occupational therapists working in an acute pediatric hospital. Reflexive thematic analysis was employed for data analysis. Results Stage one themes; (1) Lack of clarity around coaching definition, (2) Acute setting barriers to coaching, (3) Family acceptance and appropriateness, and (4) Enablers for coaching. Stage two themes; (1) Addressing skepticism about coaching, (2) Logistics and approvals, and (3) Implementation strategies for coaching. In Stage Two, participants and researchers developed an implementation plan. Conclusion Occupational therapists perceived coaching as hard to implement in acute pediatric settings due to acuity of caseloads and traditional medical models. The six-step implementation plan aims to enhance therapist knowledge and motivation as well as reduce environmental barriers, with the aim of embedding coaching into acute pediatric settings.
Effect of citrulline malate supplementation on muscle function and bioenergetics during short-term repeated bouts of fatiguing exercise

ABSTRACT Citrulline malate (CM) has been shown to improve muscle performance in healthy participants during a single exercise session. Yet, within the framework of exercises repeated at close time interval, the consequences of CM ingestion on mechanical performance are controversial and the bioenergetics side remains undocumented. The aim of this double-blind placebo-controlled study was to evaluate in vivo the effect of short-term (7 doses in 48 h) oral administration of CM upon gastrocnemius muscle function and bioenergetics using non-invasive multimodal NMR techniques in healthy rats. The experimental protocol consisted of two 6-min bouts of fatiguing exercise spaced by an 8-min recovery period. CM treatment did not affect the basal bioenergetics status and increased the half-fatigue time during the first exercise bout. With exercise repetition, it prevented PCr cost alteration and decreased both the glycolytic ATP production and the contractile ATP cost in working muscle, but these changes were not associated to any improvement in mechanical performance. In addition, CM did not influence the replenishment of high-energy phosphorylated compounds during the post-exercise recovery periods. Therefore, short-term CM administration enhances muscle bioenergetics throughout fatiguing bouts of exercise repeated at close time interval but this enhancement does not benefit to mechanical performance.
Expression pattern of resynthesized allotetraploid Capsella is determined by hybridization, not whole‐genome duplication

Summary Polyploidization, the process leading to the increase in chromosome sets, is a major evolutionary transition in plants. Whole‐genome duplication (WGD) within the same species gives rise to autopolyploids, whereas allopolyploids result from a compound process with two distinct components: WGD and interspecific hybridization. To dissect the instant effects of WGD and hybridization on gene expression and phenotype, we created a series of synthetic hybrid and polyploid Capsella plants, including diploid hybrids, autotetraploids of both parental species, and two kinds of resynthesized allotetraploids with different orders of WGD and hybridization. Hybridization played a major role in shaping the relative expression pattern of the neo‐allopolyploids, whereas WGD had almost no immediate effect on relative gene expression pattern but, nonetheless, still affected phenotypes. No transposable element‐mediated genomic shock scenario was observed in either neo‐hybrids or neo‐polyploids. Finally, WGD and hybridization interacted and the distorting effects of WGD were less strong in hybrids. Whole‐genome duplication may even improve hybrid fertility. In summary, while the initial relative gene expression pattern in neo‐allotetraploids was almost entirely determined by hybridization, WGD only had trivial effects on relative expression patterns, both processes interacted and had a strong impact on physical attributes and meiotic behaviors.
Colchicine pre-treatment and post-treatment does not worsen bleeding or functional outcome after collagenase-induced intracerebral hemorrhage

Patients with intracerebral hemorrhage (ICH) are at increased risk for major ischemic cardiovascular and cerebrovascular events. However, the use of preventative antithrombotic therapy can increase the risk of ICH recurrence and worsen ICH-related outcomes. Colchicine, an anti-inflammatory agent, has the potential to mitigate inflammation-related atherothrombosis and reduce the risk of ischemic vascular events. Here we investigated the safety and efficacy of colchicine when used both before and acutely after ICH. We predicted that daily colchicine administration would not impact our safety measures but would reduce brain injury and improve functional outcomes associated with inflammation reduction. To test this, 0.05 mg/kg colchicine was given orally once daily to rats either before or after they were given a collagenase-induced striatal ICH. We assessed neurological impairments, intra-parenchymal bleeding, Perls positive cells, and brain injury to gauge the therapeutic impact of colchicine on brain injury. Colchicine did not significantly affect bleeding (average = 40.7 μL) at 48 hrs, lesion volume (average = 24.5 mm3) at 14 days, or functional outcome (median neurological deficit scale score at 2 days post-ICH = 4, i.e., modest deficits) from 1–14 days after ICH. Colchicine reduced the volume of Perls positive cells in the perihematomal zone, indicating a reduction in inflammation. Safety measures (body weight, food consumption, water consumption, hydration, body temperature, activity, and pain) were not affected by colchicine. Although colchicine did not confer neuroprotection or functional benefit, it was able to reduce perihematomal inflammation after ICH without increasing bleeding. Thus, our findings suggest that colchicine treatment is safe, unlikely to worsen bleeding, and is unlikely but may reduce secondary injury after an ICH if initiated early post ICH to reduce the risk of ischemic vascular events. These results are informative for the ongoing CoVasc-ICH phase II randomized trial (NCT05159219).
Crystal structure determination, molecular docking and dynamics of arylidene cyanoacetates as potential JNK-3 inhibitors for Ischemia reperfusion injury

Abstract Ischemia reperfusion injury is a cardiovascular condition which causes hypoxia by means of obstruction of arterial blood flow eventually leads to reduced synthesis of adenosine tri-phosphate in the mitochondria. c-Jun N-terminal kinase-3 are related to several cascade of events like apoptosis, oxidative stress and mitochondrial dysfunction which can be further related to Ischemia-reperfusion injury. The present study was aimed at determining crystal structure of the ligand by x-ray methods and to perform molecular docking and molecular dynamics studies of the arylidene cyano-acetates with c-Jun N-terminal kinase-3. The binding energy of Ethyl (2E)-2-cyano-3-(4-methoxyphenyl)prop-2-enoate is −4.462 kcal/mol and ethyl (2E)-2-cyano-3-phenylprop-2-enoate is −6.135 kcal/mol. This has created a new rational approach to drug design, where the structure of drug is designed, based on its fit to structures of receptor site, rather than basing it on analogies to other active structures. The above compounds are binding strongly with c-Jun N-terminal kinase-3 protein. Communicated by Ramaswamy H. Sarma
Active Optical Zoom for Tracking

Abstract : In order to optically vary the magnification of an imaging system, continuous mechanical zoom lenses require multiple optical elements and use fine mechanical motion to precisely adjust the separations between groups of lenses. By incorporating active elements into the optical design, imaging systems that are capable of variable optical magnification with no macroscopic moving parts are possible. Changing the effective focal length and magnification of an imaging system can be accomplished by positioning two or more active optics in an optical design. In this application, the active optics (deformable mirrors) serve as variable focal-length lenses and steering mirrors making an active optical zoom system that can zoom in on off-axis points on the image and therefore track objects in the field of view of the system. We will present results from a bench top system.
CORRELATIONS BETWEEN DISEASE SEVERITY AND REHABILITATION OUTCOMES IN PATIENTS RECOVERING FROM COVID-19 INFECTION

Objective Based on long-term follow-up of patients with COVID-19, to evaluate whether the severity of acute COVID-19 infection affects rehabilitation outcomes. Design Observational cohort study. Subjects A total of 61 post-acute COVID-19 patients underwent inpatient and outpatient customized rehabilitation treatment. Methods The severity of acute COVID-19 infection was measured with the World Health Organization Clinical Progression Scale (WHO-CPS). Motor, cognitive, and functional variables were measured using standard and specified scales 6 months or more after acute illness. Results Of the 61 subjects, 65.6% had severe disease according to WHO-CPS. Significant improvement was found in activities of daily living functions (Functional Independence Measure (FIM) at admission 103.7 ± 18.9 vs FIM at discharge 118.7 ± 6.8) (p < 0.00). Of participants, 88% were able to wean off oxygen completely. A significant correlation was found between higher WHO-CPS, prolonged acute hospitalization, and days of ventilation were correlated with lower total and motor FIM at admission, but not with cognitive FIM or Montreal Cognitive Assessment (MoCA). No correlation was found between WHO-CPS, prolonged acute hospitalization and day of ventilation and funnctional level at discharge. Conclusion The severity of acute COVID-19 infection affects the functional status of survivors at admission to rehabilitation, but, contrary to expectations, not the functional outcomes at discharge. These findings show that even patients with severe acute COVID-19 infection may improve their daily functioning significantly during rehabilitation program. LAY ABSTRACT Many patients worldwide develop severe complications of recent infection with the coronavirus SARS-CoV-2 (COVID-19). These complications include respiratory, motor, cognitive, and functional symptoms. Rehabilitation plays an important role in the recovery of these patients. This study examined whether there is a correlation between the severity of acute COVID-19 infection and the functional level of survivors after rehabilitation. Study participants were 61 post-acute COVID-19 patients who received inpatient and outpatient rehabilitation. Most of the patients improved significantly in daily functions following rehabilitation, and most did not need oxygen support at discharge. Patients with severe COVID-19 infection started the rehabilitation period at a low functional level, but improved significantly during rehabilitation, and at discharge there was no difference between patients with more severe or less severe COVID-19 infections. These findings show that even patients with severe acute COVID-19 infection may improve significantly during rehabilitation program.
Advanced nano biosensors for rapid detection of zoonotic bacteria

An infectious disease that is transmitted from animals to humans and vice‐versa is called zoonosis. Bacterial zoonotic diseases can re‐emerge after they have been eradicated or controlled and are among the world's major health problems which inflict tremendous burden on healthcare systems. The first step to encounter such illnesses can be early and precise detection of bacterial pathogens to further prevent the following losses due to their infections. Although conventional methods for diagnosing pathogens, including culture‐based, polymerase chain reaction‐based, and immunological‐based techniques, benefit from their advantages, they also have their own drawbacks, for example, taking long time to provide results, and requiring laborious work, expensive materials, and special equipment in certain conditions. Consequently, there is a greater tendency to introduce simple, innovative, quicker, accurate, and low‐cost detection methods to effectively characterize the causative agents of infectious diseases. Biosensors, therefore, seem to practically be one of those novel promising diagnostic tools on this aim. These are effective and reliable elements with high sensitivity and specificity, that their usability can even be improved in medical diagnostic systems when empowered by nanoparticles. In the present review, recent advances in the development of several bio and nano biosensors, for rapid detection of zoonotic bacteria, have been discussed in details.
Rethinking Aversive Personality: Decomposing the Dark Triad Traits into their Common Core and Unique Flavors.

OBJECTIVE To explain and predict unethical behavior, much attention has been devoted to the "Dark Triad of Personality", a set of three socially aversive personality traits: Machiavellianism, narcissism, and psychopathy. Despite its popularity, research on the Dark Triad has been beset by recurring concerns surrounding the distinctiveness of its components. Herein, we propose an alternative theoretical view that conceptualizes the Dark Triad traits as specific manifestations of the common core of aversive traits (conceptualized as the Dark Factor of Personality, D) flavored by unique, essentially non-aversive characteristics. METHOD In two studies (total N > 1,000), we test this idea by examining the conceptual and empirical overlap and specificity of the Dark Triad traits vis-à-vis D and each other. RESULTS Findings support the conceptualization of the Dark Triad traits as flavored manifestations of D and also reveal a marked discrepancy between the current conceptualization of the Dark Triad traits and the empirical structure of its subdimensions. In fact, evidence clearly failed to support the existence of a triad of traits. CONCLUSION Conceptualizing single aversive traits as a conjunction of core D aspects and essentially non-aversive characteristics (admiration; disinhibition vs. planfulness; vulnerability vs. boldness) holds promise to move the field forward.
Structural Design and Non-linear Modeling of a Highly Stable Multi-Rotor Hovercraft

This paper presents a new design for a Multi-rotor Unmanned Air Vehicle (UAV). The design is based on the requirement of highly stable hover capability other than typical requirements of vertical takeoff and landing (VTOL), forward and sidewise motions etc. Initially a typical Tri-rotor hovercraft is selected for modeling and analysis. Then design modifications are done to improve the hover stability, with special emphasis on compensating air drag moments that exist at steady state hover. The modified structure is modeled and dynamic equations are derived for it. These equations are analyzed to verify that our structural modifications have the intended stability improvement effect during steady state hover. Keywords: Multi-rotor Crafts, T-Copter, Rotational Matrix, Pseudo Inertial Matrix, Coriolis Acceleration, Air drag moment, Swash Plate
Caught in the act—Visualizing ribonucleases during eukaryotic ribosome assembly

Ribosomes are essential macromolecular machines responsible for translating the genetic information encoded in mRNAs into proteins. Ribosomes are composed of ribosomal RNAs and proteins (rRNAs and RPs) and the rRNAs fulfill both catalytic and architectural functions. Excision of the mature eukaryotic rRNAs from their precursor transcript is achieved through a complex series of endoribonucleolytic cleavages and exoribonucleolytic processing steps that are precisely coordinated with other aspects of ribosome assembly. Many ribonucleases involved in pre‐rRNA processing have been identified and pre‐rRNA processing pathways are relatively well defined. However, momentous advances in cryo‐electron microscopy have recently enabled structural snapshots of various pre‐ribosomal particles from budding yeast (Saccharomyces cerevisiae) and human cells to be captured and, excitingly, these structures not only allow pre‐rRNAs to be observed before and after cleavage events, but also enable ribonucleases to be visualized on their target RNAs. These structural views of pre‐rRNA processing in action allow a new layer of understanding of rRNA maturation and how it is coordinated with other aspects of ribosome assembly. They illuminate mechanisms of target recognition by the diverse ribonucleases involved and reveal how the cleavage/processing activities of these enzymes are regulated. In this review, we discuss the new insights into pre‐rRNA processing gained by structural analyses and the growing understanding of the mechanisms of ribonuclease regulation.
Selection of Reference Genes for Normalization of qRT–PCR Analysis in the Soybean Aphid Aphis glycines Matsumura (Hemiptera: Aphididae)

Abstract The soybean aphid Aphis glycines Matsumura is a predominant insect pest in Asia and North America and causes great losses to soybean. The release of genome data for A. glycines will facilitate gene function research in the future. However, suitable reference genes for A. glycines under various experimental conditions are scarce. To search for appropriate reference genes for A. glycines, nine candidate reference genes, including Act, α-Tub, β-Tub, RPS12, RPS18, RPL5, RPL27, EF1α, and Fer, were tested under six experimental conditions to evaluate their suitability for use in the normalization of qRT–PCR data. Results showed that EF1α and RPS12 were optimal for the developmental stages of A. glycines, RPS18 and RPS12 were appropriate for wing dimorphism, β-Tub and RPS18 were suitable for different tissues and RPL5, and α-Tub could be used for normalization at different temperatures. β-Tub and EF1α could be proposed as reference genes for insecticide treatment, and RPL5 and RPS12 were found to be the most stable reference genes in different photoperiods. The results provide appropriate reference genes for analyzing gene expression in A. glycines and contribute to future research on the molecular physiology and biochemistry of A. glycines.
Morphotropic phase boundary and electrical properties of K1−xNaxNbO3 lead-free ceramics

Lead-free K1−xNaxNbO3 ceramics with x=0.48–0.54 were prepared by a conventional solid-state reaction method to investigate the influence of the K/Na ratio on phase structures and electrical properties. The results suggest that a typical morphotropic phase boundary exists at x=0.52–0.525, separating the monoclinic and orthorhombic phases. The sample with the composition near x=0.52 shows the maximum values of the piezoelectric constant (d33=160 pC/N) and the electromechanical coupling coefficient (kt=47%). The results provide a helpful guidance to consider the optimal ratio of K to Na for designing and developing new (K,Na)NbO3-based ceramics.
A comprehensive survey of the approaches for pathway analysis using multi-omics data integration

Abstract Pathway analysis has been widely used to detect pathways and functions associated with complex disease phenotypes. The proliferation of this approach is due to better interpretability of its results and its higher statistical power compared with the gene-level statistics. A plethora of pathway analysis methods that utilize multi-omics setup, rather than just transcriptomics or proteomics, have recently been developed to discover novel pathways and biomarkers. Since multi-omics gives multiple views into the same problem, different approaches are employed in aggregating these views into a comprehensive biological context. As a result, a variety of novel hypotheses regarding disease ideation and treatment targets can be formulated. In this article, we review 32 such pathway analysis methods developed for multi-omics and multi-cohort data. We discuss their availability and implementation, assumptions, supported omics types and databases, pathway analysis techniques and integration strategies. A comprehensive assessment of each method’s practicality, and a thorough discussion of the strengths and drawbacks of each technique will be provided. The main objective of this survey is to provide a thorough examination of existing methods to assist potential users and researchers in selecting suitable tools for their data and analysis purposes, while highlighting outstanding challenges in the field that remain to be addressed for future development.
Trend-following with better adaptation to large downside risks

Avoiding losses from long-term trend reversals is challenging, and trend-following is one of the few trading approaches to explore it. While trend-following is popular among investors and has gained increased attention in academia, the recent diminished profitability in equity markets casts doubt on its effectiveness. To clarify its cause and suggest remedies, we thoroughly examine the effect of market conditions and averaging window on recent profitability using four major stock indices in an out-of-sample experiment comparing trend-following rules (moving average and momentum) and a machine-classification-based non-trend-following rule. In addition to the significant advantage of trend-following rules in avoiding downside risks, we find a discrepancy in the optimum averaging window size between trend direction phases, which is exacerbated by a higher positive trend direction ratio. A higher positive trend direction ratio leads to poor performance relative to buy-and-hold returns. This discrepancy creates the dilemma of choosing which trend direction phase to emphasize. Incorporating machine-learning into trend-following is effective for alleviating this dilemma. We find that the profit-maximizing averaging window realizes the level that best balances the dilemma and suggest a simple guideline for selecting the optimum averaging window. We attribute the sluggishness of trend-following in recent equity markets to the insufficient chances of trend reversals rather than their loss of profitability. Our results contribute to improving the performance of trend following by mitigating the dilemma.
Reward-motivated memories influence new learning across development

Previously rewarding experiences can influence choices in new situations. Past work has demonstrated that existing reward associations can either help or hinder future behaviors and that there is substantial individual variability in the transfer of value across contexts. Developmental changes in reward sensitivity may also modulate the impact of prior reward associations on later goal-directed behavior. The current study aimed to characterize how reward associations formed in the past affected learning in the present from childhood to adulthood. Participants completed a reinforcement learning paradigm using high- and low-reward stimuli from a task completed 24 h earlier, as well as novel stimuli, as choice options. We found that prior high-reward associations impeded learning across all ages. We then assessed how individual differences in the prioritization of high- versus low-reward associations in memory impacted new learning. Greater high-reward memory prioritization was associated with worse learning performance for previously high-reward relative to low-reward stimuli across age. Adolescents also showed impeded early learning regardless of individual differences in high-reward memory prioritization. Detrimental effects of previous reward on choice behavior did not persist beyond learning. These findings indicate that prior reward associations proactively interfere with future learning from childhood to adulthood and that individual differences in reward-related memory prioritization influence new learning across age.