Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis

After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a previously reported observation that additional copies of the binding region, introduced into the chromosome using an integrative plasmid, were also predominantly particle bound. Subsections of the original plasmid insertion were cloned into the integrative plasmid and introduced into B. subtilis, in which they became tandemly reiterated under appropriate selective conditions. HaeIII sites in the vector, flanking each insertion, were used to excise the latter for subsequent tests of particle association. Examination of 10 strains containing subsections of the original 5.2-kilobase-pair region showed that the binding region was confined to 283 base pairs. This was confirmed by dissection in vitro of a larger, isolated, particle-bound sequence. The nucleotide sequence of a 1,300-base-pair region that contained this site was determined. The entire region had a notably high A + T content and was deficient in open reading frames for transcription.
Perifosine – a new option in treatment of acute myeloid leukemia?

Introduction: Perifosine is a novel targeted oral Akt inhibitor. In preclinical leukemia models, perifosine has an independent cytotoxic potential but also synergizes well with other rationally selected targeted agents. The evidence from clinical trials supporting the use of perifosine in the therapy of leukemias is limited. The optimal dose and schedule have yet to be defined. However, given its favorable toxicity profile and mechanism of action, the therapeutic potential of perifosine should be evaluated in well-designed clinical trials. Areas covered: The role of the phosphatidylinositol-3 kinase (PI3K)/Akt zpathway in normal cells, cancer and leukemias is discussed. The mechanism of action of perifosine and the basic information on the development and chemical properties are summarized. The evidence from in vivo and in vitro studies is presented. The efficacy and side effect profile are summarized. Expert opinion: The safety and tolerability profile of perifosine are satisfactory. The evidence from clinical trials in patients with leukemias is very limited. The preclinical data are encouraging. Perifosine has the potential to play a role in the treatment of leukemias in the future. Its role needs to be confirmed in clinical trials.
Efficient Tariff Structures for Distribution Network Services

This paper reviews different methods for ensuring economic efficiency when devising tariffs for distribution network services while meeting the revenue constraint.11For further discussions about different regulatory frameworks in regulating network tariffs, the reader can refer to Nepal et al. (2014). There is a long tradition in welfare economics which suggests that prices should be based on long run marginal costs to achieve economic efficiency. However, network revenue requirements are often based on embedded costs, which are typically higher than long run marginal costs. This creates the problem of recovering residual costs. There are several methods available for doing so. Of course, economic efficiency is not the only criterion for designing tariffs. Fairness and gradualism are two other criteria that play heavily in the design of electricity tariffs. By drawing upon representative data from Australia, we illustrate the various methods and evaluate their likely performance on these criteria.
Intracellular trafficking of influenza virus: clinical implications for molecular medicine

The trafficking and processing steps that occur in cells that are infected with influenza virus play a crucial role in the outcome of infection. These steps are targets for new and future anti-viral drugs, and can affect the relative virulence of the virus and its ability to cause disease. The virus first binds to its host cell via specific sialic acid residues, which can control the species tropism of the virus. The internalisation of the virus, into the nucleus of the cell, is dependent on a low pH, and this process is therapeutically targeted by the drug amantadine. Following replication, the newly formed viral genomes leave the nucleus and assemble into infectious particles at the plasma membrane. The targeting and processing of the various viral components at this late stage of the infectious cycle can have a major effect on the ability of the virus to spread and cause disease in its host. Finally, the release of viruses is dependent on the enzyme neuraminidase (NA), and this function has recently been targeted by the NA inhibitors, a new generation of drugs against influenza virus.
Omnidirectional guided wave inspection of large metallic plate structures using an EMAT array

The design of an electromagnetic acoustic transducer (EMAT) array device for the inspection of large areas of metallic plate-like structures using the S/sub 0/ guided wave mode is described. The reasons for using the S/sub 0/ mode are discussed and it is shown how the choice of mode determines the nature of the EMAT array elements. A novel array construction technique is shown to be necessary whereby the EMAT coils for adjacent elements are overlapped in order to achieve the required element density. Results are presented that illustrate the operation of the device on steel and aluminum plate specimens in the thickness range from 5 to 10 mm. An area of at least 10 m/sup 2/ can be inspected from a single location. Spurious signals in the results are caused both by the unwanted A/sub 0/ mode and by S/sub 0/ sidelobes, the latter occurring at the same radial distance from the array as the genuine S/sub 0/ signal from a reflector, but in the wrong direction. The signal-to-coherent noise performance of the complete system is determined by the amplitude ratio of the largest genuine S/sub 0/ signal to the largest spurious signal. This is typically around 30 dB. The sensitivity of the device to artificial defects and genuine corrosion patches is demonstrated and the limitations of its operation are discussed. The feasibility of using the device with the S/sub 1/ guided wave mode to inspect a 20 mm thick plate is also demonstrated.
Subjective memory complaints in preclinical autosomal dominant Alzheimer disease

Objective: To cross-sectionally study subjective memory complaints (SMC) in autosomal dominant Alzheimer disease (ADAD). Methods: We examined self-reported and study partner–based SMC in 52 young, cognitively unimpaired individuals from a Colombian kindred with early-onset ADAD. Twenty-six carried the PSEN-1 E280A mutation, averaging 7 years of age younger than the kindred's expected clinical onset. Twenty-six were age-matched noncarriers. Participants also underwent structural MRI and cognitive testing. Results: Self-reported SMC were greater in carriers than noncarriers (p = 0.02). Study partner–based SMC did not differ between groups (p = 0.21), but in carriers increased with age (r = 0.66, p < 0.001) and decreased with hippocampal volume (r = −0.35, p = 0.08). Conclusions: Cognitively unimpaired PSEN-1 carriers have elevated SMC. Self-reported SMC may be a relatively early indicator of preclinical AD, while partner- reported SMC increases later in preclinical AD, closer to clinical onset.
Social Capital and the Willingness to Become Self-Employed: Is There a Difference between Women and Men?

This article studies the role of social capital in the occupational choice process involving whether to become self-employed or not. Although the decision to become self-employed has itself been analyzed frequently, social capital as an important explanatory variable has often been neglected. We show that the willingness to become self-employed depends quite strongly on a person's social capital. Furthermore, we are able to show for the first time that since women invest differently in social capital than men, this factor helps to explain gender differences in the willingness to become self-employed. We test our hypotheses with two ordered probit estimates using a data set with 5,000 students. Firstly, we find that the more social capital individuals hold, the more willing they are to start their own business. Secondly, we observe that women invest differently and on a smaller scale in social capital than men and are therefore less willing to become self-employed. This helps to explain why the entrepreneurship rate of women is still substantially lower than that of men and underlines the importance of fostering the inclusion of women in networking activities and other events generating social capital.
Extreme genetic risk for type 1A diabetes

Type 1A diabetes (T1D) is an autoimmune disorder the risk of which is increased by specific HLA DR/DQ alleles [e.g., DRB1*03-DQB1*0201 (DR3) or DRB1*04-DQB1*0302 (DR4)]. The genotype associated with the highest risk for T1D is the DR3/4-DQ8 (DQ8 is DQA1*0301, DQB1*0302) heterozygous genotype. We determined HLA-DR and -DQ genotypes at birth and analyzed DR3/4-DQ8 siblings of patients with T1D for identical-by-descent HLA haplotype sharing (the number of haplotypes inherited in common between siblings). The children were clinically followed with prospective measurement of anti-islet autoimmunity and for progression to T1D. Risk for islet autoimmunity dramatically increased in DR3/4-DQ8 siblings who shared both HLA haplotypes with their diabetic proband sibling (63% by age 7, and 85% by age 15) compared with siblings who did not share both HLA haplotypes with their diabetic proband sibling (20% by age 15, P < 0.01). 55% sharing both HLA haplotypes developed diabetes by age 12 versus 5% sharing zero or one haplotype (P = 0.03). Despite sharing both HLA haplotypes with their proband, siblings without the HLA DR3/4-DQ8 genotype had only a 25% risk for T1D by age 12. The risk for T1D in the DR3/4-DQ8 siblings sharing both HLA haplotypes with their proband is remarkable for a complex genetic disorder and provides evidence that T1D is inherited with HLA-DR/DQ alleles and additional MHC-linked genes both determining major risk. A subset of siblings at extremely high risk for T1D can now be identified at birth for trials to prevent islet autoimmunity.
Regulation of WRN Protein Cellular Localization and Enzymatic Activities by SIRT1-mediated Deacetylation*

Werner syndrome is an autosomal recessive disorder associated with premature aging and cancer predisposition caused by mutations of the WRN gene. WRN is a member of the RecQ DNA helicase family with functions in maintaining genome stability. Sir2, an NAD-dependent histone deacetylase, has been proven to extend life span in yeast and Caenorhabditis elegans. Mammalian Sir2 (SIRT1) has also been found to regulate premature cellular senescence induced by the tumor suppressors PML and p53. SIRT1 plays an important role in cell survival promoted by calorie restriction. Here we show that SIRT1 interacts with WRN both in vitro and in vivo; this interaction is enhanced after DNA damage. WRN can be acetylated by acetyltransferase CBP/p300, and SIRT1 can deacetylate WRN both in vitro and in vivo. WRN acetylation decreases its helicase and exonuclease activities, and SIRT1 can reverse this effect. WRN acetylation alters its nuclear distribution. Down-regulation of SIRT1 reduces WRN translocation from nucleoplasm to nucleoli after DNA damage. These results suggest that SIRT1 regulates WRN-mediated cellular responses to DNA damage through deacetylation of WRN.
Are Family Businesses Really Different? European Experiences from STRATOS

Using observations of 1,132 small- and medium-sized enterprises in eight European countries, a comparison is made of family and nonfamily businesses. The variables compared concern values and attitudes, objectives, and strategic behavior. The data reveal that family businesses are inwardly directed or closed family-related systems. Among their managers are fewer pioneers than “all-rounders” and organizers; as a consequence, their strategic behavior is rather conservative. Therefore, family businesses should be viewed as stable rather than progressive or dynamic factors of the economy.
Observations on the Macroscopic Digestive Anatomy of the Himalayan Tahr (Hemitragus jemlahicus)

We measured macroscopic anatomical parameters of the digestive tract of 12 female Himalayan tahrs. According to morphological criteria used sensu Hofmann for the classification of ruminants into feeding types – in particular the reticuloruminal capacity measured as wet content weights, the ruminal pillar thickness, the reticular honeycomb structure, the size of the omasum and the size of the parotid glands – tahrs can be classified as predominantly grazing ruminants. As reported in other ruminant species, the length of the large intestine was a more constant parameter over a range of body weights than that of the small intestine. The weight of the parotid glands scaled to metabolic body weight.
Maternal seafood diet, methylmercury exposure, and neonatal neurologic function.

OBJECTIVE To determine whether neonatal neurologic function is adversely affected by seafood contaminants from maternal diet during pregnancy. STUDY DESIGN One hundred eighty-two singleton term births were evaluated in the Faeroe Islands, where marine food includes pilot whale. Maternal serum, hair, and milk and umbilical cord blood were analyzed for contaminants. Levels of essential fatty acids, selenium, and thyroid hormones were determined in cord blood. Each infant's neurologic optimality score was determined at 2 weeks of age adjusted for gestational age, and predictors were assessed by regression analysis. RESULTS Exposures to methylmercury and polychlorinated biphenyls were increased in relation to maternal seafood intake, as were omega3 fatty acid concentrations in cord serum. Thyroid function was normal. After adjustment for confounders, a 10-fold increase of the cord-blood mercury concentration was associated with a decreased neurologic optimality score of 2.0 (P =. 03). This effect corresponds to a decrease in gestational age of about 3 weeks. Other indicators of the seafood diet had no effect on this outcome. CONCLUSIONS Prenatal exposure to methylmercury from contaminated seafood was associated with an increased risk of neurodevelopmental deficit. Thus in this North Atlantic population, methylmercury constituted an important neurologic risk factor, although effects of other seafood components were not detectable.
Globalizing European Union environmental policy

This contribution explores the European Union's (EU) efforts to ‘globalize’ EU environmental regulation. EU leadership on global environmental governance emerged as a result of the combined effects of domestic politics and international regulatory competition. The growing power of environmental interests in Europe from the late 1980s, coupled with dynamics of EU policy-making, led the EU to commit to ambitious environmental policies. Given this commitment, it was in the EU's international competitive interests to support international agreements that would pressure other jurisdictions to adopt similar environmental regulations. Promoting treaties that spread EU environmental norms internationally also served to legitimize EU rules and to shield them from legal challenges before world trade bodies.
Dynamics of Innovation in an Open Source Collaboration Environment: Lurking, Laboring, and Launching Floss Projects on Sourceforge

A systems analysis perspective is adopted to examine the critical properties of the Free/Libre/Open Source Software (FLOSS) mode of innovation, as reflected on the SourceForge platform (SF.net). This approach re-scales March’s (1991) framework and applies it to characterize the “innovation system” of a “distributed organization” of interacting agents in a virtual collaboration environment. The innovation system of the virtual collaboration environment is an emergent property of two “coupled” processes: one involves interactions among agents searching for information to use in designing novel software products, and the other involves the mobilization of individual capabilities for application in the software development projects. Micro-dynamics of this system are studied empirically by constructing transition probability matrices representing movements of 222,835 SF.net users among 7 different activity states. Estimated probabilities are found to form first-order Markov chains describing ergodic processes. This makes it possible to computate the equilibrium distribution of agents among the states, thereby suppressing transient effects and revealing persisting patterns of project-joining and project-launching.
Income Sensitivity of Price Elasticities: Comment

Noam (1981) claims to derive a measure for the &dquo;income elasticity of price elasticity&dquo; and to estimate this for eight categories of public goods. The first section of this comment shows that Noam’s derivation is incorrect and that for the functional form Noam used for demand, price elasticity is insensitive to income. Nevertheless, other functional forms would allow price elasticity to vary with income. One important implication of this possibility is discussed in the second section of the article.
Misoprostol attenuates acetic acid-induced increases in mucosal permeability and inflammation: role of blood flow.

The objectives of this study were 1) to quantify the effects of misoprostol (Miso; prostaglandin E1 analogue) on acetic acid-induced increases in mucosal permeability and inflammation; 2) to determine what effect acetic acid, Miso, or the combination of Miso plus acetic acid has on colonic blood flow; and 3) to assess whether the protective effect of Miso may be attributable to its vasodilatory properties. We found that intrarectal administration of acetic acid produced a 6.4-fold increase in colonic myeloperoxidase activity (an index of granulocyte infiltration), an 8.2-fold increase in mucosal permeability, a 1.6-fold increase in colonic weight, and a 6.8% decrease in body weight 48 h after enema. Miso pretreatment significantly attenuated the increases in colonic myeloperoxidase activity, mucosal permeability, and colon weight as well as prevented the loss of body weight. In a different series of experiments, we found that blood flow in the descending, transverse, and ascending colon increased 2.5- to 3.5-fold immediately after the acetic acid enema; however, it returned to control values at 1 and 4 h after enema. Miso pretreatment, followed by acetic acid, resulted in a further increase (2.5-fold) in blood flow in the descending colon 1 h after enema compared with acetic acid alone. This Miso-induced increase in blood flow at 1 h could not account for its protective effect inasmuch as colonic mucosal permeability (i.e., injury) in Miso-pretreated animals was not significantly different from values obtained in animals pretreated with vehicle and then given the enema.
Contingent Premium Options

This article describes the valuation, hedging and applications of contingent premium options (CPOs). We derive closed-form formulas for path independent and path dependent CPOs that address most cases of practical importance. The resulting hedge ratios show that when it is not possible to hedge these options statically, hedging CPOs may become problematic, especially when the options approach maturity. We also discuss how to structure more specific CPOs, such as money-back and pay-later options. For an investor with a strong view on the price of the underlying asset, these types of options may be an attractive alternative to standard options.
Unexpected Importance of Potential Parasites in the Composition of the Freshwater Small-Eukaryote Community

ABSTRACT The diversity of small eukaryotes (0.2 to 5 μm) in a mesotrophic lake (Lake Bourget) was investigated using 18S rRNA gene library construction and fluorescent in situ hybridization coupled with tyramide signal amplification (TSA-FISH). Samples collected from the epilimnion on two dates were used to extend a data set previously obtained using similar approaches for lakes with a range of trophic types. A high level of diversity was recorded for this system with intermediate trophic status, and the main sequences from Lake Bourget were affiliated with ciliates (maximum, 19% of the operational taxonomic units [OTUs]), cryptophytes (33%), stramenopiles (13.2%), and cercozoa (9%). Although the comparison of TSA-FISH results and clone libraries suggested that the level of Chlorophyceae may have been underestimated using PCR with 18S rRNA primers, heterotrophic organisms dominated the small-eukaryote assemblage. We found that a large fraction of the sequences belonged to potential parasites of freshwater phytoplankton, including sequences affiliated with fungi and Perkinsozoa. On average, these sequences represented 30% of the OTUs (40% of the clones) obtained for each of two dates for Lake Bourget. Our results provide information on lacustrine small-eukaryote diversity and structure, adding to the phylogenetic data available for lakes with various trophic types.
Corporate Governance Reforms in Malaysia: The Key Leading Players' Perspectives

This paper focuses on the corporate governance reforms in Malaysia since the 1997/1998 Asian financial crisis. Drawing upon ten in-depth semi-structured interviews conducted with leading players who were highly involved in Malaysia's corporate governance development, together with a review of the literature in this area, the paper offers evidence on the meaning of corporate governance in the Malaysian context, the factors behind the recent reforms and views on the appropriate corporate governance system for Malaysia. While it is shown that Malaysia's corporate governance reforms has modelled on the Anglo-American systems to a large extent, the majority of the interviewees placed greater emphasis on the social aspect of corporate governance in contrast to the traditional notion of shareholder accountability. The main concern raised in the paper is that without changes in the previous problematic corporate culture, the intended purpose of the recent corporate governance reforms will unlikely to be achieved.
Late onset asthma clinical and immunological data: importance of allergy.

In people over 65 years of age (mean age 72) late onset asthma (LOA) is often mistaken for chronic obstructive pulmonary disease (COPD). Furthermore, it is generally believed that true allergic asthma starts very seldom in old age and that, in this case, it is actually intrinsic asthma i.e., without any sensitization to aeroallergens. The aim of this study was to show that LOA is not an uncommon entity and is often allergic. Among a population of 1,888 subjects (over 65 years of age) in a general medicine ward we found 21 patients (1.1%) corresponding to this entity. The diagnosis of asthma was assessed according to clinical data, pulmonary function and nasal provocation tests, and blood eosinophilia. The diagnosis of atopy was assessed on total IgE, prick tests and RAST for the commonest allergens of our area. This group of 21 patients was compared to three groups of age-matched subjects including: 68 patients afflicted with chronic bronchitis, a group of 14 patients who had asthma before the age of 65 (presenile asthma), and 20 normal controls. A group of 15 asthmatics under age 65 was also included (young asthma mean age 37). We found a rather high incidence of allergy (72%), mostly to Dermatophagoides pteronyssinus, but also to the pollens Parietaria judaica, grasses and Olea europea. Although the average age (except in the young asthma group) and the severity of symptoms were the same in the four groups, unexpectedly mean RAST and skin test values were higher in LOA than in the two groups of young asthmatics and presenile asthmatics. It was concluded that LOA is not uncommon among elderly patients over 65, which is in contrast with most previous studies; 72% of our cases were allergic, the main allergens being mite, and Parietaria judaica pollen.