Miracle-dz/UniFAR-FTUs huggingface dataset:
The Polish adaptation of the measurements of rule-governed behaviors: Generalized Pliance Questionnaire, Generalized Tracking Questionnaire and Generalized Self-Pliance Questionnaire

In some circumstances rule-governed behavior, a behavior that is governed by verbal rules instead of environmental consequences, may be beneficial for human beings. At the same time, rigid rule following is associated with psychopathology. Thus measurement of rule-governed behavior may be of special use in a clinical setting. The aim of this paper is to assess the psychometric properties of Polish adaptations of three questionnaires measuring generalized tendency to engage in various types of rule-governed behaviors: Generalized Pliance Questionnaire (GPQ), Generalized Self-Pliance Questionnaire (GSPQ), Generalized Tracking Questionnaire (GTQ). A forward-backward method was used for translation. Data was collected from two samples: general population (N = 669) and university students (N = 451). To measure the validity of the adapted scales the participants filled in a set of self-assessed questionnaires: Satisfaction with Life Scale (SWLS), Depression, Anxiety, and Stress Scale– 21 (DASS-21), General Self-Efficacy Scale (GSES), Acceptance and Action Questionnaire–II (AAQ-II), Cognitive Fusion Questionnaire (CFQ), Valuing Questionnaire (VQ) and Rumination—Reflection Questionnaire (RRQ). The exploratory and confirmatory analyses confirmed the unidimensional structure of each of the adapted scales. All of those scales presented good reliability (internal consistency measured with Cronbach Alpha) and item-total correlations. The Polish versions of questionnaires presented significant correlations in the expected directions with relevant psychological variables in line with the original studies. The measurement occurred invariant across both samples as well as gender. The results provide evidence that Polish versions of GPQ, GSPQ and GTQ present sufficient validity and reliability to be used in the Polish-speaking population.
Predicting coarse-grained semantic features in language comprehension: evidence from ERP representational similarity analysis and Chinese classifier

Abstract Existing studies demonstrate that comprehenders can predict semantic information during language comprehension. Most evidence comes from a highly constraining context, in which a specific word is likely to be predicted. One question that has been investigated less is whether prediction can occur when prior context is less constraining for predicting specific words. Here, we aim to address this issue by examining the prediction of animacy features in low-constraining context, using electroencephalography (EEG), in combination with representational similarity analysis (RSA). In Chinese, a classifier follows a numeral and precedes a noun, and classifiers constrain animacy features of upcoming nouns. In the task, native Chinese Mandarin speakers were presented with either animate-constraining or inanimate-constraining classifiers followed by congruent or incongruent nouns. EEG amplitude analysis revealed an N400 effect for incongruent conditions, reflecting the difficulty of semantic integration when an incompatible noun is encountered. Critically, we quantified the similarity between patterns of neural activity following the classifiers. RSA results revealed that the similarity between patterns of neural activity following animate-constraining classifiers was greater than following inanimate-constraining classifiers, before the presentation of the nouns, reflecting pre-activation of animacy features of nouns. These findings provide evidence for the prediction of coarse-grained semantic feature of upcoming words.
Velocity-Based Method in Free-Weight and Machine-Based Training Modalities: The Degree of Freedom Matters

Supplemental Digital Content is Available in the Text. Abstract Hernández-Belmonte, A, Buendía-Romero, Á, Pallares, JG, and Martínez-Cava, A. Velocity-based method in free-weight and machine-based training modalities: the degree of freedom matters. J Strength Cond Res 37(9): e500–e509, 2023—This study aimed to analyze and compare the load-velocity relationships of free-weight and machine-based modalities of 4 resistance exercises. Moreover, we examined the influence of the subject's strength level on these load-velocity relationships. Fifty men completed a loading test in the free-weight and machine-based modalities of the bench press, full squat, shoulder press, and prone bench pull exercises. General and individual relationships between relative intensity (%1RM) and velocity variables were studied through the coefficient of determination (R2) and standard error of the estimate (SEE). Moreover, the velocity attained to each %1RM was compared between both modalities. Subjects were divided into stronger and weaker to study whether the subject's strength level influences the mean test (mean propulsive velocity [MPVTest]) and 1RM (MPV1RM) velocities. For both modalities, very close relationships (R2 ≥ 0.95) and reduced estimation errors were found when velocity was analyzed as a dependent (SEE ≤ 0.086 m·s−1) and independent (SEE ≤ 5.7% 1RM) variable concerning the %1RM. Fits were found to be higher (R2 ≥ 0.995) for individual load-velocity relationships. Concerning the between-modality comparison, the velocity attained at each intensity (from 30 to 100% 1RM) was significantly faster for the free-weight variant. Finally, nonsignificant differences were found when comparing MPVTest (differences ≤ 0.02 m·s−1) and MPV1RM (differences ≤ 0.01 m·s−1) between stronger and weaker subjects. These findings prove the accuracy and stability of the velocity-based method in the free-weight and machine-based variants but highlight the need to use the load-velocity relationship (preferably the individual one) specific to each training modality.
Feller operators and mixture priors in Bayesian nonparametrics

Priors for Bayesian nonparametric inference on a continuous curve are often defined through approximation techniques, e.g. basis-functions expansions with random coefficients. Using constructive approximations is particularly attrac- tive, since it may facilitate the prior elicitation. With this motivation we study a class of operators, introduced by Feller, for the constructive approximation of a bounded real function. Feller operators have a simple, probabilistic structure. We prove that, when the random elements used in their construction are chosen in the natural exponential family, they have several properties of interest in statistical applications, and can be represented as mixtures of simple probability distribution functions. As a by-product, we give some new results on the natural exponential family. Our construction offers more insights on the role of mixtures in Bayesian nonparametrics. A fairly general class of mixture priors arises, which includes con- tinuous, countable, or finite mixtures, with kernels suggested by the approximation scheme. This allows the study of theoretical properties in a unified setting; in par- ticular, we give results on the Kullback-Leibler property for the proposed class of mixture priors, and on the consistency of the corresponding posterior, extending results known only for specific kernels.
Anticoagulant Management and Outcomes in Nontraumatic Intracranial Hemorrhage Complicated by Venous Thromboembolism: A Retrospective Chart Review

BACKGROUND There are limited data on anticoagulant management of acute venous thromboembolism (VTE) after spontaneous intracranial hemorrhage (ICH). METHODS We reviewed retrospectively all cases diagnosed with VTE during hospitalization for spontaneous ICH at our center during 15 years. Anticoagulation management outcomes were a) timing after ICH of anticoagulant initiation for VTE treatment, b) use of immediate therapeutic dosing or stepwise dose escalation, and c) proportion achieving therapeutic dose. Primary clinical efficacy outcome was recurrent VTE. Primary safety outcome was expanding ICH. RESULTS We analyzed 103 cases with VTE after 11 days (median; interquartile range [IQR] 7-22) from diagnosis of ICH. Forty patients (39%) achieved therapeutic anticoagulation 21.5 days (median; IQR 14-34 days) from the ICH. Of those, 14 (35%; 14% of total) received immediately therapeutic dose and 26 (65%; 25% of total) had stepwise escalation. Anticoagulation was more aggressive in patients with VTE >14 days after admission versus those with earlier VTE diagnosis. Twenty-two patients (21%) experienced recurrent/progressive VTE - less frequently among patients with treatment escalation within 7 days or with no escalation, than with escalation >7 days from the VTE. There were 19 deaths 6 days (median; IQR, 3.5-15) after the index VTE, with significantly higher in-hospital mortality rate among patients without escalation in anticoagulation. CONCLUSIONS Prompt therapeutic anticoagulation for acute VTE seems safe when occurring more than 14 days after spontaneous ICH. For VTE occurring earlier it might also be safe with therapeutic anticoagulation, but stepwise dose escalation to therapeutic within a 7-day period might.
Salmonella Invasion Is Controlled by Competition among Intestinal Chemical Signals

Enteric pathogens acutely sense their environment for signals to regulate their virulence functions. We demonstrate here that the enteric pathogen Salmonella utilizes the competition among certain regional intestinal constituents to modulate its virulence determinants in that region. ABSTRACT The intestine is a complex, ever-changing environment replete with an array of signaling molecules. To colonize such a complex organ, pathogens have adapted to utilize specific cues from the local environment to intricately regulate the expression of their virulence determinants. Salmonella preferentially colonizes the distal ileum, a niche enriched in the metabolite formic acid. Here, we show that the relatively higher concentration of this metabolite in the distal ileum prevents other signals from repressing Salmonella invasion in that region. We show that imported and unmetabolized formic acid functions as a cytoplasmic signal that competitively binds to HilD, the master transcriptional regulator of Salmonella invasion, thus preventing repressive fatty acids from binding to the protein. This results in an increased lifetime of HilD and subsequent derepression of invasion genes. This study demonstrates an important mechanism by which Salmonella utilizes competition among signals in the gut to its advantage as a pathogen. IMPORTANCE Enteric pathogens acutely sense their environment for signals to regulate their virulence functions. We demonstrate here that the enteric pathogen Salmonella utilizes the competition among certain regional intestinal constituents to modulate its virulence determinants in that region. We show that the high concentration of formic acid in the ileum outcompetes other signals and triggers the activation of virulence genes in the ileum. This study shows a delicate spatial and temporal mechanism by which enteric pathogens may utilize the competition among environmental cues to optimize their pathogenicity.
A systematic review of medical science students' knowledge and related factors towards burns first aids

This systematic review aims to examine the knowledge of medical science students about burn first aid. A comprehensive, systematic search was performed in different international electronic databases, such as Scopus, PubMed, Web of Science, and Persian electronic databases such as Iranmedex, and Scientific Information Database (SID) using keywords extracted from Medical Subject Headings such as “Knowledge”, “First aid”, “Medical science student” and “Burn” from the earliest to February 1, 2023. The quality of the studies included in this systematic review was evaluated by using the appraisal tool for cross‐sectional studies (AXIS tool). Thirteen studies had a total of 5046 students studying medical sciences. Among them, 62.24% were female and 77.67% were medical students. The studies included in this systematic review were conducted in Saudi Arabia, Pakistan, Syria, Egypt, and Malaysia. Medical science students' average knowledge of first aid for burns is 56.92 out of 100, which is considered to be a moderate level of knowledge. Factors such as the experience of first aid training, academic year, clinical experience, and economic status had a significant positive relationship with the knowledge of medical science students about first aid for burns. Also, factors such as age, academic specialisation, academic year, information sources, and fathers' occupation had a significant relationship with the knowledge of medical students about first aid for burns. Therefore, it is suggested that healthcare managers and policymakers make the necessary plans to ensure that first aid training courses are implemented during medical science students' studies as a part of their curriculum.
Effects of body positions on arterial stiffness as assessed by pulse wave velocity

Background: Assessing arterial stiffness through pulse wave velocity (PWV) usually requires participants to be in a supine position. If this position is not feasible, adjustments such as tilting the bed or bending the knees may be made. The Vicorder device also recommends tilting the upper body to prevent jugular vein interference in the recorded carotid pulse. Objective: To examine the impact of varying body positions on PWV. Methods: Seventy adults were studied in the fully supine (0°) to 40° upper body tilted-up positions with and without knee bend. Carotid-femoral PWV (cfPWV) was measured using two different testing devices (Omron VP-1000plus and Vicorder) and brachial-ankle PWV (baPWV) was measured using Omron. Results: cfPWV measured at 10° tilt-up was not different from 0° position while baPWV increased significantly from 10°. Elevations in cfPWV were 7% at 20° and 15% at 40° compared with 0° position. Knee bend did not affect cfPWV but decreased baPWV at each angle (P < 0.05). Jugular vein interference on the Vicorder was observed in 78% of participants in supine position, decreasing as body angle increased (7% at 30°). However, cfPWV values measured by Vicorder were consistent with those obtained by Omron even with jugular vein interference. Conclusion: Arterial stiffness assessed by PWV increased gradually and significantly in semi-Fowler's position ≥20°. Knee bend decreased baPWV but did not seem to affect cfPWV. PWV should be measured in supine position if possible. If the supine posture is not tolerated, knee bend followed by a slight incline position may be recommended.
Investigation of Peptidoglycan-Associated Lipoprotein of Acinetobacter baumannii and Its Interaction with Fibronectin To Find Its Therapeutic Potential

Acinetobacter baumannii causes hospital-acquired infections and is responsible for high mortality and morbidity. The interaction of this bacterium with the host is critical in bacterial pathogenesis and infection. ABSTRACT Acinetobacter baumannii causes hospital-acquired infections and is responsible for high mortality and morbidity. The interaction of this bacterium with the host is critical in bacterial pathogenesis and infection. Here, we report the interaction of peptidoglycan-associated lipoprotein (PAL) of A. baumannii with host fibronectin (FN) to find its therapeutic potential. The proteome of A. baumannii was explored in the host-pathogen interaction database to filter out the PAL of the bacterial outer membrane that interacts with the host’s FN protein. This interaction was confirmed experimentally using purified recombinant PAL and pure FN protein. To investigate the pleiotropic role of PAL protein, different biochemical assays using wild-type PAL and PAL mutants were performed. The result showed that PAL mediates bacterial pathogenesis, adherence, and invasion in host pulmonary epithelial cells and has a role in the biofilm formation, bacterial motility, and membrane integrity of bacteria. All of the results suggest that PAL’s interaction with FN plays a vital role in host-cell interaction. In addition, the PAL protein also interacts with Toll-like receptor 2 and MARCO receptor, which suggests the role of PAL protein in innate immune responses. We have also investigated the therapeutic potential of this protein for vaccine and therapeutic design. Using reverse vaccinology, PAL’s potential epitopes were filtered out that exhibit binding potential with host major histocompatibility complex class I (MHC-I), MHC-II, and B cells, suggesting that PAL protein is a potential vaccine target. The immune simulation showed that PAL protein could elevate innate and adaptive immune response with the generation of memory cells and would have subsequent potential to eliminate bacterial infection. Therefore, the present study highlights the interaction ability of a novel host-pathogen interacting partner (PAL-FN) and uncovers its therapeutic potential to combat infection caused by A. baumannii.
φC31 ‐Mediated cassette exchange in Sf9 insect cells for stable expression

Insect cells, especially Sf9 cells, are commonly used in biomanufacturing due to their advantages in high expression levels and post‐translational modification. However, the development of stable expression cell lines via random integration tended to be unstable. Site‐specific integration (SSI) is an alternative strategy. In this study, a φC31 ‐mediated cassette exchange system in Sf9 cells was established for SSI. The tagging cassette with the reporter gene egfp was randomly inserted into the cell genome. Potential platform cell lines were obtained by fluorescence‐activated cell sorting (FACS) and single‐cell cloning. Platform cell lines were selected by assessing the fluorescence expression, stability, and growth kinetics of cell lines. The selected platform cell lines were co‐transfected with the φC31‐containing plasmid and the targeting cassette. Green‐fluorescence‐negative clones were screened by hygromycin resistance and FACS. The resulting cell clones exhibited the expression properties of the platform cell lines. The rapid development of cell lines for the production of influenza subunit vaccines by the cassette exchange system demonstrated that the system constituted a versatile and reusable platform for the production of various recombinant proteins. Overall, the φC31‐mediated cassette exchange system in Sf9 cells has the potential to facilitate and accelerate biologics development.
Toward Right Ventricle Segmentation in Cardiac MRIs via Feature Multiplexing and Multiscale Weighted Convolution

Cardiovascular diseases are the leading cause of mortality, and accurate segmentation of ventricular regions incardiac magnetic resonance images (MRIs) is crucial for diagnosing and treating these diseases. However, fully automated and accurate right ventricle (RV) segmentation remains challenging due to the irregular cavities with ambiguous boundaries and mutably crescentic structures with relatively small targets of the RV regions in MRIs. In this article, a triple-path segmentation model, called FMMsWC, is proposed by introducing two novel image feature encoding modules, i.e., the feature multiplexing (FM) and multiscale weighted convolution (MsWC) modules, for the RV segmentation in MRIs. Considerable validation and comparative experiments were conducted on two benchmark datasets, i.e., the MICCAI2017 Automated Cardiac Diagnosis Challenge (ACDC), and the Multi-Centre, Multi-Vendor & Multi-Disease Cardiac Image Segmentation Challenge (M&MS) datasets. The FMMsWC outperforms state-of-the-art approaches, and its performance can approach that of the manual segmentation results by clinical experts, facilitating accurate cardiac index measurement for the rapid assessment of cardiac function and aiding diagnosis and treatment of cardiovascular diseases, which has great potential for clinical applications.
’More of the same, but worse than before’: A qualitative study of the challenges encountered by people who use drugs in Nova Scotia, Canada during COVID-19

Background To learn about the experiences of people who use drugs, specifically opioids, in the Halifax Regional Municipality (HRM), in Nova Scotia, Canada during the COVID-19 pandemic through qualitative interviews with people who use drugs and healthcare providers (HCP). This study took place within the HRM, a municipality of 448,500 people [1]. During the pandemic many critical services were interrupted while overdose events increased. We wanted to understand the experiences of people who use drugs as well as their HCPs during the first year of the pandemic. Methodology We conducted a qualitative study using semi-structured interviews with 13 people who use drugs and 6 HCPs, including physicians who work in addiction medicine (3), a pharmacist, a nurse, and a community-based opioid agonist therapy (OAT) program staff member. Participants were recruited within HRM. Interviews were held via phone or videoconference due to social distancing directives. Interviews focused on the challenges people who use drugs and HCPs faced during the pandemic as well as elicited perspectives on a safe supply of drugs and the associated barriers and facilitators to the provision of a safe supply. Results Of the 13 people who use drugs who participated in this study, ages ranged from 21–55 years (mean 40). Individuals had spent on average 17 years in HRM. Most people who use drugs (85%, n = 11) utilized income assistance, the Canadian Emergency Response Benefit, or disability support. Many had experienced homelessness (85%, n = 11) and almost half (46%, n = 6) were currently precariously housed in the shelter system. The main themes among interviews (people who use drugs and HCPs) were housing, accessing healthcare and community services, shifts in the drug supply, and perspectives on safe supply. Conclusions We identified several challenges that people who use drugs face in general, but especially during the COVID-19 pandemic. Access to services, housing support, and interventions to use safely at home were limited. As many challenges faced by people who use drugs exist outside of COVID-19, we concluded that the formal and informal interventions and changes in practice that were made to support people who use drugs should be sustained well past the end of the pandemic. The need for enhanced community supports and a safe supply of drugs, despite its complicated nature, is essential for the health and safety of people who use drugs in HRM, especially during COVID-19.
Sex modifies the relationship between age and neurovascular coupling in healthy adults

Neurovascular coupling (NVC) is the matching between local neuronal activity and regional cerebral blood flow (CBF), but little is known about the effects of age and sex on NVC. This study aimed to investigate the relationships and interaction between age and sex on NVC. Sixty-four healthy adults (18–85 years, N = 34 female) completed a visual stimulus evoked NVC assessment to a flashing checkerboard. NVC responses were measured in the posterior cerebral artery (PCAv) using transcranial Doppler ultrasound. A hierarchical multiple regression was used to determine the relationships between age, sex, and the age by sex interaction on NVC. There was a significant age by sex interaction for baseline (P = 0.001) and peak PCAv (P = 0.01), with a negative relationship with age in females (P < 0.005), and no relationship in males (P ≥ 0.17). NVC responses as a percent increase from baseline showed a significant age by sex interaction (P = 0.014), with a positive relationship with age in females (P = 0.04) and no relationship in males (P = 0.17), even after adjusting for baseline PCAv. These data highlight important sex differences, with an association between age and NVC only apparent in females but not males, and thus a need to account for sex dependent effects of ageing when investigating cerebrovascular regulation.