Miracle-dz/UniFAR-FTUs huggingface dataset:
Short-Term Repeated-Dose Toxicity Profile of Archaeosomes Administered to Mice via Intravenous and Oral Routes

Archaeosomes, liposomes made from polar ether lipids of archaea, show promise for vaccine and drug delivery applications. The potential toxicity of intravenously (14,70, or 140 mg/kg/day for 5 consecutive days) and orally (gavaged at 55,275, or 550 mg/kg/day for 10 consecutive days) administered unilamellar archaeosomes, prepared from the total polar lipids (TPLs) extracted from several species of archaea, was assessed in female BALB/c mice. Liposomes prepared from an ester phospholipid composition were included for comparative purposes. Control groups of mice were administered 0.1 ml phosphate-buffered saline (PBS) by either route. Animals were monitored at least once daily for temperature, body weight, and clinical signs of adverse reactions. One day after the last dose, the mice were sacrificed. Blood was collected for selected biochemical/enzyme analyses, and the major organs (heart, lungs, liver, spleen, kidneys) were weighed and examined macroscopically. In addition, the spleens were examined histologically. At the two lower dosages of intravenously administered vesicles, there were no significant indications of toxicity, as compared with the PBS-administered control group. At the highest intravenous dose of 140 mg/kg/day, archaeosomes prepared from the TPL of the extreme halophiles, Halobacterium salinarum and Natronobacterium magadii, indicated potential toxicity, as evidenced by clinical signs (hyperactivity and/or piloerection), drop in body temperature, and loss in body weight. Spleens from mice administered some archaeosomes types, primarily at the highest intravenous dose tested, were enlarged, had increased organ weight, and microscopic examination revealed mild to moderate expansion of the red pulp with increased numbers of hematopoietic cells, but no changes in the white pulp. There were similar clinical signs at one or more of the higher oral doses of the ester liposomes and some of the archaeosome types; however, no other apparent toxicity was observed. Based on this limited mouse study. archaeosomes were generally well tolerated after intravenous or oral delivery at the dosages so indicated in this study.
Predictive role of capillaroscopic skin ulcer risk index in systemic sclerosis: a multicentre validation study

Introduction The early detection of systemic sclerosis (SSc) patients at high risk of developing digital ulcers could allow preventive treatment, with a reduction of morbidity and social costs. In 2009, a quantitative score, the capillaroscopic skin ulcer risk index (CSURI), calculated according to the formula ‘D×M/N2’, was proposed, which was highly predictive of the appearance of scleroderma digital ulcers within 3 months of capillaroscopic evaluation. Objectives This multicentre study aims to validate the predictive value and reproducibility of CSURI in a large population of SSc patients. Methods CSURI was analysed in 229 unselected SSc patients by nailfold videocapillaroscopy (NVC). All patients were re-evaluated 3 months later with regard to the persistence and/or appearance of new digital ulcers. Results 57 of 229 patients presented with digital ulcers after 3 months. The receiver operating characteristic curve analysis showed an area under the curve of 0.884 (95% CI 0.835 to 0.922), with specificity and sensitivity of 81.4% (95% CI 74.8 to 86.89) and 92.98% (95% CI 83.0 to 98.0), respectively, at the cut-off value of 2.96. The reproducibility of CSURI was validated on a random sample of 81 patients, with a κ-statistic measure of interrater agreement of 0.8514. Conclusions The role of CSURI was confirmed in detecting scleroderma patients with a significantly high risk of developing digital ulcers within the first 3 months from NVC evaluation. CSURI is the only method validated to predict the appearance of digital ulcers and its introduction into routine clinical practice might help optimise the therapeutic strategy of these harmful SSc complications.
Objective Quantification of Convective Clustering Observed During the AMIE/DYNAMO Two‐Day Rain Episodes

One critical bottleneck in developing and evaluating ways to represent the mesoscale organization of convection in cumulus parameterization schemes is that there is no single accepted method of objectively quantifying the degree of convective organization or clustering from observations. This study addresses this need using high‐quality S‐PolKa radar data from the Atmospheric Radiation Measurement Madden‐Julian Oscillation Investigation Experiment/Dynamics of the Madden‐Julian Oscillation (AMIE/DYNAMO) field campaign. We first identify convective elements (contiguous convective echoes [CCEs]) from radar reflectivity observations using the rain type classification algorithm of Powell et al. (2016, https://doi.org/10.1175/JTECH‐D‐15‐0135.1). Then we apply scalar clustering metrics, including the organization index (Iorg) of Tompkins and Semie, to the radar CCEs to test their ability of quantifying convective clustering during the observed two‐day rain episodes. Our results show two distinct phases of convective clustering during the two‐day rain episodes, with each phase covering about 10 hr before (Phase 1) and after (Phase 2) the time of peak rain rate. In Phase 1 clustering, the number of CCEs increases and convective cells cluster as new cells form preferentially near existing convective entities. The number of CCEs decreases as the environment stabilizes in Phase 2 clustering, during which already clustered cells with associated stratiform clouds are preferred over the isolated ones. Iorg is capable of capturing convective clustering in both phases. The possible mechanisms for convective clustering are discussed, including cold pool‐updraft feedback, moisture‐convection interaction, and mesoscale circulations. Our results suggest that parameterizations of convective organization should represent the feedback processes that are responsible for the convective clustering during both phases.
Memcapacitor based charge pump

This paper proposes a charge pump based on a charge controlled memcapacitor. The operation of the charge pump is investigated along with the mathematical analysis of the memcapacitor. Different implementations of charge pump are summarized. The proposed charge pump has the capability of driving low input voltage in range of 200mv and the capability of operating at the low frequencies which makes it suitable for biomedical applications.
Macrophage-Derived LIF and IL1B Regulate Alpha(1,2)Fucosyltransferase 2 (Fut2) Expression in Mouse Uterine Epithelial Cells During Early Pregnancy1

Macrophages accumulate within stromal tissue subjacent to the luminal epithelium in the mouse uterus during early pregnancy after seminal fluid exposure at coitus. To investigate their role in regulating epithelial cell expression of fucosylated structures required for embryo attachment and implantation, fucosyltransferase enzymes Fut1, Fut2 (Enzyme Commission number [EC] 2.4.1.69), and Fut4 (EC 2.4.1.214) and Muc1 and Muc4 mRNAs were quantified by quantitative real-time PCR in uterine epithelial cells after laser capture microdissection in situ or after epithelial cell coculture with macrophages or macrophage-secreted factors. When uterine macrophage recruitment was impaired by mating with seminal plasma-deficient males, epithelial cell Fut2 expression on Day 3.5 postcoitus (pc) was reduced compared to intact-mated controls. Epithelial cell Fut2 was upregulated in vitro by coculture with macrophages or macrophage-conditioned medium (MCM). Macrophage-derived cytokines LIF, IL1B, and IL12 replicated the effect of MCM on Fut2 mRNA expression, and MCM-stimulated expression was inhibited by anti-LIF and anti-IL1B neutralizing antibodies. The effects of acute macrophage depletion on fucosylated structures detected with lectins Ulex europaeus 1 (UEA-1) and Lotus tetragonolobus purpureas (LTP), or LewisX immunoreactivity, were quantified in vivo in Cd11b-dtr transgenic mice. Depletion of macrophages caused a 30% reduction in luminal epithelial UEA-1 staining and a 67% reduction in LewisX staining in uterine tissues of mice hormonally treated to mimic early pregnancy. Together, these data demonstrate that uterine epithelial Fut2 mRNA expression and terminal fucosylation of embryo attachment ligands is regulated in preparation for implantation by factors including LIF and IL1B secreted from macrophages recruited during the inflammatory response to insemination.
Auditory-Motor Learning during Speech Production in 9-11-Year-Old Children

Background Hearing ability is essential for normal speech development, however the precise mechanisms linking auditory input and the improvement of speaking ability remain poorly understood. Auditory feedback during speech production is believed to play a critical role by providing the nervous system with information about speech outcomes that is used to learn and subsequently fine-tune speech motor output. Surprisingly, few studies have directly investigated such auditory-motor learning in the speech production of typically developing children. Methodology/Principal Findings In the present study, we manipulated auditory feedback during speech production in a group of 9–11-year old children, as well as in adults. Following a period of speech practice under conditions of altered auditory feedback, compensatory changes in speech production and perception were examined. Consistent with prior studies, the adults exhibited compensatory changes in both their speech motor output and their perceptual representations of speech sound categories. The children exhibited compensatory changes in the motor domain, with a change in speech output that was similar in magnitude to that of the adults, however the children showed no reliable compensatory effect on their perceptual representations. Conclusions The results indicate that 9–11-year-old children, whose speech motor and perceptual abilities are still not fully developed, are nonetheless capable of auditory-feedback-based sensorimotor adaptation, supporting a role for such learning processes in speech motor development. Auditory feedback may play a more limited role, however, in the fine-tuning of children's perceptual representations of speech sound categories.
IL‐17A increases TNF‐α‐induced COX‐2 protein stability and augments PGE2 secretion from airway smooth muscle cells: impact on β2‐adrenergic receptor desensitization

IL‐17A plays an important role in respiratory disease and is a known regulator of pulmonary inflammation and immunity. Recent studies have linked IL‐17A with exacerbation in asthma and COPD. We have shown that the enzyme cyclooxygenase‐2 (COX‐2) and its prostanoid products, prostaglandin E2 (PGE2) in particular, are key contributors in in vitro models of infectious exacerbation; however, the impact of IL‐17A was not known.
Fault Detection for Nonlinear Systems With Multitone Sinusoidal Input

As sinusoidal inputs are common in engineering systems, it is therefore important to develop fault detection methods for these systems in order to improve their reliability. In this paper, a fault detection method for nonlinear systems with multitone sinusoidal input is developed. By assuming the output of the system has the almost periodicity property, the proposed method is derived based on the periodogram for fault detection using the asymptotic local approach, which is effective to detect both incipient and abrupt faults. It is shown that the fault detection problem using the proposed method is reduced to a chi2-test for a given confidence level. Simulation example involving a nonlinear system is used to illustrate the implementation and the performance of the proposed method.
Efficient Parallel CKY Parsing on GPUs

Low-latency solutions for syntactic parsing are needed if parsing is to become an integral part of user-facing natural language applications. Unfortunately, most state-of-the-art constituency parsers employ large probabilistic context-free grammars for disambiguation, which renders them impractical for real-time use. Meanwhile, Graphics Processor Units (GPUs) have become widely available, offering the opportunity to alleviate this bottleneck by exploiting the fine-grained data parallelism found in the CKY algorithm. In this paper, we explore the design space of parallelizing the dynamic programming computations carried out by the CKY algorithm. We use the Compute Unified Device Architecture (CUDA) programming model to reimplement a state-of-the-art parser, and compare its performance on two recent GPUs with different architectural features. Our best results show a 26-fold speedup compared to a sequential C implementation.
Asymmetric Dimethyarginine as Marker and Mediator in Ischemic Stroke

Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS) inhibitor, is known as mediator of endothelial cell dysfunction and atherosclerosis. Circulating ADMA levels are correlated with cardiovascular risk factors such as hypercholesterolemia, arterial hypertension, diabetes mellitus, hyperhomocysteinemia, age and smoking. Accordingly, clinical studies found evidence that increased ADMA levels are associated with a higher risk of cerebrovascular events. After the acute event of ischemic stroke, levels of ADMA and its analog symmetric dimethylarginine (SDMA) are elevated through augmentation of protein methylation and oxidative stress. Furthermore, cleavage of ADMA through dimethylarginine dimethylaminohydrolases (DDAHs) is reduced. This increase of dimethylarginines might be predictive for adverse clinical outcome. However, the definite role of ADMA after acute ischemic stroke still needs to be clarified. On the one hand, ADMA might contribute to brain injury by reduction of cerebral blood flow. On the other hand, ADMA might be involved in NOS-induced oxidative stress and excitotoxic neuronal death. In the present review, we highlight the current knowledge from clinical and experimental studies on ADMA and its role for stroke risk and ischemic brain injury in the hyperacute stage after stroke. Finally, further studies are warranted to unravel the relevance of the close association of dimethylarginines with stroke.
Investing in Family Planning: Key to Achieving the Sustainable Development Goals

Voluntary family planning brings transformational benefits to women, families, communities, and countries. Investing in family planning is a development “best buy” that can accelerate achievement across the 5 Sustainable Development Goal themes of People, Planet, Prosperity, Peace, and Partnership. Voluntary family planning brings transformational benefits to women, families, communities, and countries. Investing in family planning is a development “best buy” that can accelerate achievement across the 5 Sustainable Development Goal themes of People, Planet, Prosperity, Peace, and Partnership.
Diacylated Sulfoglycolipids Are Novel Mycobacterial Antigens Stimulating CD1-restricted T Cells during Infection with Mycobacterium tuberculosis

Mycobacterial lipids comprise a heterogeneous group of molecules capable of inducing T cell responses in humans. To identify novel antigenic lipids and increase our understanding of lipid-mediated immune responses, we established a panel of T cell clones with different lipid specificities. Using this approach we characterized a novel lipid antigen belonging to the group of diacylated sulfoglycolipids purified from Mycobacterium tuberculosis. The structure of this sulfoglycolipid was identified as 2-palmitoyl or 2-stearoyl-3-hydroxyphthioceranoyl-2′-sulfate-α-α′-d-trehalose (Ac2SGL). Its immunogenicity is dependent on the presence of the sulfate group and of the two fatty acids. Ac2SGL is mainly presented by CD1b molecules after internalization in a cellular compartment with low pH. Ac2SGL-specific T cells release interferon γ, efficiently recognize M. tuberculosis–infected cells, and kill intracellular bacteria. The presence of Ac2SGL-responsive T cells in vivo is strictly dependent on previous contact with M. tuberculosis, but independent from the development of clinically overt disease. These properties identify Ac2SGL as a promising candidate to be tested in novel vaccines against tuberculosis.
SKIP Is a Component of the Spliceosome Linking Alternative Splicing and the Circadian Clock in Arabidopsis[W]

This work shows that SKIP, a spliceosomal component, is critical for clock function in Arabidopsis thaliana. Loss of SKIP function impairs splicing on a genomic scale, and skip shows multiple defects in circadian clock function, including altered period, temperature compensation, and light sensitivity. Circadian clocks generate endogenous rhythms in most organisms from cyanobacteria to humans and facilitate entrainment to environmental diurnal cycles, thus conferring a fitness advantage. Both transcriptional and posttranslational mechanisms are prominent in the basic network architecture of circadian systems. Posttranscriptional regulation, including mRNA processing, is emerging as a critical step for clock function. However, little is known about the molecular mechanisms linking RNA metabolism to the circadian clock network. Here, we report that a conserved SNW/Ski-interacting protein (SKIP) domain protein, SKIP, a splicing factor and component of the spliceosome, is involved in posttranscriptional regulation of circadian clock genes in Arabidopsis thaliana. Mutation in SKIP lengthens the circadian period in a temperature-sensitive manner and affects light input and the sensitivity of the clock to light resetting. SKIP physically interacts with the spliceosomal splicing factor Ser/Arg-rich protein45 and associates with the pre-mRNA of clock genes, such as PSEUDORESPONSE REGULATOR7 (PRR7) and PRR9, and is necessary for the regulation of their alternative splicing and mRNA maturation. Genome-wide investigations reveal that SKIP functions in regulating alternative splicing of many genes, presumably through modulating recognition or cleavage of 5′ and 3′ splice donor and acceptor sites. Our study addresses a fundamental question on how the mRNA splicing machinery contributes to circadian clock function at a posttranscriptional level.
Energy Consumption, Employment and the Kyoto Protocol in New Zealand: Searching for Causality

This paper considers the causal relationship between employment, energy consumption and economic growth using a range of different approaches. The study finds long-run neutrality in energy consumption, except for electricity and oil consumption where there is evidence of uni-directional linkages from electricity consumption to employment and from oil consumption to employment. We also found that there is uni-directional link from real GDP growth to employment. These conclusions are robust across the different methodologies and have implications of environmental and energy issues including the Kyoto Protocol.
Linear regression motion analysis for unsupervised temporal segmentation of human actions

One of the biggest dificulties in human action analysis is the temporal complexity and structure of actions. By breaking actions down into smaller temporal pieces, it may be possible to enhance action recognition, or allow unsupervised temporal action clustering. We propose a temporal segmentation system for human action recognition based on person tracking and a novel segmentation algorithm. We apply optical flow, PCA, and linear regression error estimation to human action videos to get a metric, L', that can be used to split an action into several more easily recognised subactions. The L' metric can be effectively calculated and is robust. To validate the semantic coherence of the sub-actions, we represent the sub-actions as features using a variant of the Motion History Image and perform action recognition experiments on two popular datasets, the KTH and the MSR2. Our results demonstrate that the algorithm performs well, showing promise for future application in action clustering and action retrieval tasks.
Design and Fabrication of Heat Storage Thermoelectric Harvesting Devices

Thermoelectric energy harvesting requires a substantial temperature difference ΔT to be available within the device structure. This has restricted its use to particular applications such as heat engine structural monitoring, where a hot metal surface is available. An alternative approach is possible in cases where ambient temperature undergoes regular variation. This involves using a heat storage unit, which is filled with a phase-change material (PCM), to create an internal spatial temperature difference from temperature variation in time. In this paper, key design parameters and a characterization methodology for such devices are defined. The maximum electrical energy density expected for a given temperature range is calculated. The fabrication, characterization, and analysis of a heat storage harvesting prototype device are presented for temperature variations of a few tens of degrees around 0 °C, corresponding to aircraft flight conditions. Output energy of 105 J into a 10- Ω matched resistive load, from a temperature sweep from +20 °C to -21 °C, then to +25 °C is demonstrated, using 23 g of water as the PCM. The proposed device offers a unique powering solution for wireless sensor applications involving locations with temperature variation, such as structural monitoring in aircraft, industrial, and vehicle facilities.
Stochastic H∞ control problem with state-dependent noise for multimodeling systems

In this paper, stochastic H∞ state feedback control with state-dependent noise for multimodeling systems is addressed. After establishing the asymptotic structure of the stochastic multi-modeling algebraic Riccati equation (SMARE), an iterative algorithm that is based on Newton's method is established. A high-order state feedback controller by means of the obtained iterative solution is given and the degradation of the H∞ performance is investigated for the stochastic case for the first time. Finally, in order to demonstrate the efficiency of the proposed algorithm, numerical example is given for practical megawatt-frequency control problem.
A fully parallel mortar finite element projection method for the solution of the unsteady Navier-Stokes equations

This paper describes the parallel implementation of a mortar finite-element projection method to compute in- compressible viscous flows. The basic idea in the derivation of this method is that the appropriate functional setting for pro- jection methods must accommodate two different spaces for representing the two velocity fields calculated in the viscous and incompressible half steps of the method. The velocity cal- culated in the viscous step is chosen in the space constrained by the mortar elements; that is, a weak continuity through the subdomain interfaces is enforced, whereas in the projection step, the weak continuity is relaxed. As a result, the projec- tion step is fully parallel. The numerical solutions of a series of test problems in two dimensions calculated by the pro- posed method compare quite satisfactorily with the reference solutions.
Episiotomy characteristics and risks for obstetric anal sphincter injuries: a case-control study

Please cite this paper as: Stedenfeldt M, Pirhonen J, Blix E, Wilsgaard T, Vonen B, Øian P. Episiotomy characteristics and risks for obstetric anal sphincter injuries: a case‐control study. BJOG 2012;119:724–730.