Miracle-dz/UniFAR-FTUs huggingface dataset:
Electrical Stimulation of the Prefrontal Cortex Increases Cholecystokinin, Glutamate, and Dopamine Release in the Nucleus Accumbens: an In Vivo Microdialysis Study in Freely Moving Rats

In vivo microdialysis, radioimmunoassay, and HPLC with electrochemical or fluorometric detection were used to investigate the release of cholecystokinin (CCK), glutamate (Glu), and dopamine (DA) in nucleus accumbens septi (NAS) as a function of ipsilateral electrical stimulation of medial prefrontal cortex (mPFC). CCK was progressively elevated by mPFC stimulation at 50–200 Hz. Stimulation-induced CCK release was intensity-dependent at 250–700 μA. NAS Glu and DA levels were each elevated by stimulation at 25–400 Hz; the dopamine metabolites DOPAC and homovanillic acid were increased by stimulation at 100–400 Hz. When rats were trained to lever press for mPFC stimulation, the stimulation induced similar elevations of each of the three transmitters to those seen with experimenter-administered stimulation. Perfusion of 1 mm kynurenic acid (Kyn) into either the ventral tegmental area (VTA) or NAS blocked lever pressing for mPFC stimulation. VTA, but not NAS, perfusion of Kyn significantly attenuated the increases in NAS DA levels induced by mPFC stimulation. Kyn did not affect NAS CCK or Glu levels when perfused into either the VTA or NAS. The present results are consistent with histochemical evidence and provide the first in vivoevidence for the existence of a releasable pool of CCK in the NAS originating from the mPFC. Although dopamine is the transmitter most closely linked to reward function, it was CCK that showed frequency-dependent differences in release corresponding most closely to rewarding efficacy of the stimulation. Although not essential for the reward signal itself, coreleased CCK may modulate the impact of the glutamatergic action in this behavior.
Antimalarial Activities of Aminooxy Compounds

ABSTRACT Twenty-three aminooxy compounds have been examined for their ability to inhibit the growth of the malaria parasite Plasmodium falciparum in vitro. Eight of these compounds were found to have 50% inhibitory concentrations less than 10 μM, with the best drugs being canaline (the aminooxy analogue of ornithine) and CGP51905A at 297 ± 23.6 nM and 242 ± 18.8 nM, respectively. Canaline was also assayed in combination with the ornithine decarboxylase inhibitor difluoromethylornithine, and the two drugs were found to be synergistic in antimalarial activity.
A Novel Channel Estimation in T-DMB Receivers

This paper proposes noise reduction methods to improve the channel estimation performance in terrestrial-digital multimedia broadcasting (T-DMB) receivers. In the T-DMB, the channel estimation is performed by the pilot signal extraction and the channel coefficient estimation. Since the performance of the channel estimation highly depends on the estimated channel coefficients whose accuracy is associated with the received signal-to-noise ratio (SNR), it is important to reduce noise existing at the estimated channel coefficients. To reduce such noise components, various noise reduction methods of the estimated channel coefficients based on the Ad-point weighted moving average are proposed. Computer simulations show that the proposed methods improve the estimation performance by more than 2 to 3 dB in terms of the symbol error rate after equalization.
Investigation of determinant factors of minimum operating voltage of logic gates in 65-nm CMOS

Determinant factors of the minimum operating voltage (V<inf>DDmin</inf>) of CMOS logic gates are investigated by measurements of logic-gate chains in 65nm CMOS. V<inf>DDmin</inf> consists of a systematic component (V<inf>DDmin(SYS)</inf>) and a random variation component (V<inf>DDmin(RAND)</inf>). V<inf>DDmin(SYS)</inf> is minimized, when the logic threshold voltage of logic gates equals to half supply voltage (V<inf>DD</inf>). The tuning of the logic threshold voltage of each logic gate is achieved by the sizing of the gate width of nMOS/pMOS. V<inf>DDmin(RAND)</inf> is minimized by reducing the random threshold variation achieved by increasing the gate width or the forward body biasing. In addition, the temperature dependence of V<inf>DDmin</inf> is measured for the first time. The temperature for the worst corner analysis for V<inf>DDmin</inf> should be changed depending on the number of gate counts of logic circuits.
THE IMPORTANCE OF FREQUENTLY REPEATED ARTERIAL BLOOD GAS STUDIES IN SEVERE CHRONIC BRONCHITIS: REPORT OF A CASE

Oxygen therapy in patients with chronic respiratory disease and respiratory failure is known to be difficult to handle, and may be compared to insulin therapy in patients with brittle diabetes. In both diseases the clinical evidence and general rules of treatment may be unsatisfactory for the proper management of individual cases. Thus certain rapidly performed and frequently repeated laboratory tests may be of life-saving importance. Recent technical improvements make it possible to determine arterial pH, Pco, and Po, (partial pressures of carbon dioxide and oxygen) in ten to fifteen minutes of work in the laboratory. In the following case the laboratory data directed the treatment towards a favorable outcome. The relative importance of the bronchitis versus the emphysema will be discussed.
Multi-sensory modalities for music learning

This paper describes a study that investigates the human senses important for music learning. The objective is to identify the appropriate modalities for musical applications. A survey was conducted to seek users’ feedback mapping suitable human senses to various types of musical instruments, and ranking the senses according to their importance. Results suggested that touch sensation is considered the most important modality when playing musical instruments. The paper concludes with a discussion on the need to use suitable multi-sensory modalities when designing systems for music learning.
The Contraceptive Revolution

Here is the full report of the 1970 National Fertility Study, a national sample survey for which thousands of women were interviewed who had been married at some time and were of reproductive age when they were interviewed. The book assesses the growth in the use of the pill and the IUD, the increasing reliance on contraceptive sterilization, and both the intended and the unwanted fertility of American women. The volume opens with an introduction to the survey and its methods. Contraceptive practice in 1970 is then compared with data for 1965, and an analysis is supplied of trends since 1955 in the attitudes of Roman Catholics.Originally published in 1977.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These paperback editions preserve the original texts of these important books while presenting them in durable paperback editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Kinetics and energetics of subunit dissociation/unfolding of TIM: the importance of oligomerization for conformational persistence and chemical stability of proteins.

Kinetics of unfolding and refolding of rabbit muscle triosephosphate isomerase (TIM) were measured as a function of guanidine hydrochloride (GdnHCl) concentration. From the rate constants of these processes, the activation free-energy barriers (delta G++) were calculated using the Arrhenius equation. Assuming a linear dependence of delta G++ on the concentration of GdnHCl, activation energies in the absence of GdnHCl were estimated. The Gibbs free-energy change of dissociation/unfolding (delta G) was determined from GdnHCl unfolding curves in equilibrium. Using these data and the literature value for the bimolecular association rate constant of folded TIM monomers [Zabori, S. , Rudolph, R. , and Jaenicke, R. (1980) Z. Naturforsch. 35C, 999-1004], a model was developed that fully describes both kinetics and energetics of subunit dissociation/unfolding of TIM. Unfolded TIM monomers are susceptible to proteolytic digestion and thiol oxidation, while native TIM is resistant to both. The present model explains how the dimeric nature of TIM decreases the frequency of subunit unfolding by several orders of magnitude, thus increasing the chemical stability of the protein. Furthermore, the model also explains the recently demonstrated persistence (on a time scale of hours to days) of conformational heterogeneity of native TIM dimers [Rietveld, A. W. M. , and Ferreira, S. T. (1996) Biochemistry 35, 7743-7751]. Again, it appears that the dimeric nature of TIM is essential for this behavior.
Does a public sphere exist in the European Union? An analysis of the content of the debate on the Haider case

This article sets out how the public sphere can be studied through an analysis of the content of a specific debate. A public discourse can be said to pertain to a European Union-wide public sphere where the discourse within the EU is significantly different from that developed in non-EU countries, where such differences are not nationally defined, and where the debates in individual newspapers (which provide the fora for a public sphere) should be connected on the basis of some underlying factors. These conditions are tested with a quantitative analysis of the newspaper debate in 1999 and 2000 on the sanctions of the EU-14 against Austria. To the extent that the conditions are found, it can be concluded that there exists a European public opinion. The objective of studying this specific case is to demonstrate that, as far as an EU issue is concerned, there are already signs of an EU transnational political community.
Athletic Expenditures and the Academic Mission of American Schools: A Group-Level Analysis

Most studies find positive correlations at the individual level of analysis between athletic participation and academic success. One opportunity for scholarship left largely unexplored concerns the effect of athletics on group-level processes. The author used a resource-based perspective to explore the influence of athletic investment on academic achievement at the organizational level. Data were collected from 227 school districts. Multiple regression analyses revealed negative but insignificant relationships between athletic expenditures and indicators of basic skills and college preparation. Future research might determine whether the nonassociation observed in this study between athletic spending and academic performance generalizes to different school settings.
The feeling of knowing a word's meaning

Subjects were presented with rare words, and required to define them. Feeling-of-knowing judgements were made with respect to meaning of words that could not be defined accurately. It was found that subjects’ feeling-of-knowing judgements accurately predicted their performance on semantic-differential and related-word tasks designed to measure connotative and denotative aspects of word meaning, respectively. In addition, number of semantically related words recognized was consistently related to both feeling-of-knowing judgements and to semantic-differential performance. It was concluded that feeling-of-knowing judgements and their predictive accuracy were result of knowledge of semantic attributes or features.
From the Subjects' Point of View

In personnel selection, situational judgment tests are based on the principle of simulation: The applicant is confronted with a typical work situation and has to decide what kind of behavior is appropriate. In two studies, we investigate the subjects' reactions to different kinds of situational judgment items. The item formats examined differ with regard to two variables: Interactivity (noninteractive: In each item, the subject is confronted with a new situation vs. interactive: The situation develops according to the answer given and the subject is asked once more about the new situation) as well as modality of presentation (stimulus and response components of the items are given in the form of a video vs. in text form). We expected the degree of interaction and the presentation via video to have a positive effect on the subjects' evaluation (in terms of usefulness, emotional reaction, transparency, job-relatedness, acceptance, fairness). In accordance with our expectations, interactive situational judgm.
Energy in Electromagnetism: The Poynting Vector [Historical Corner]

John Henry Poynting (1852-1914) [Figure 1(a)] was born in Lancashire, England, and he was the youngest son of Thomas Elford Poynting, a Unitarian minister who was also the teacher in the local school that young John Henry attended. Poynting later attended Owens College in Manchester, England (1867-1872), where he studied physics under the guidance of Osborne Reynolds (1842-1912) and Balfour Stewart (1828-1887).
Risk Factors of Ischemic Cardiac Disease in Patients on Continuous Ambulatory Peritoneal Dialysis

Elevated plasma levels of fibrinogen, factor VII coagulant activity (F VIIc), and plasminogen activator inhibitor (PAI-1) have been reported to be strictly associated with thrombotic events and are considered to be important risk markers of atherothrombotic caridovascular disease. Therefore, we evaluated in 15 patients on continuous ambulatory peritoneal dialysis (CAPD) the plasma levels of these coagulation factors, basal insulin values, and the lipid pattern in comparison with 33 hemodialysis (HD) patients and 59 healthy subjects. In CAPD the total cholesterol and triglyceride results were significantly increased, but no difference was found in HDL cholesterol. Fibrinogen and F VIIc results were significantly higher In CAPD and HD than In the control group, probably due to an increased hepatic synthesis as a nonspecific response to the peritoneal protein loss. Elevated F VIIc activity may be caused by the presence of large negatively charged lipoproteins, in viva thrombin formation, or reduced hepatic clearance. Both PAl 1 and t-PA results were higher in CAPD, probably due to an increased synthesis by endothelial cells activated by glucose peritoneal absorption and hypertonic dialysis solutions. The contemporary elevation of fibrinogen, F VIIc, PAI-1, and t-PA suggests that CAPD patients present a hypercoagulability and hypofibrinolysis condition, which may promote the development of atherothrombotic events.
Frontiers in therapeutic development of allopregnanolone for Alzheimer’s disease and other neurological disorders

Allopregnanolone (Allo), a neurosteroid, has emerged as a promising promoter of endogenous regeneration in brain. In a mouse model of Alzheimer’s disease, Allo induced neurogenesis, oligodendrogenesis, white matter generation and cholesterol homeostasis while simultaneously reducing β-amyloid and neuroinflammatory burden. Allo activates signaling pathways and gene expression required for regeneration of neural stem cells and their differentiation into neurons. In parallel, Allo activates systems to sustain cholesterol homeostasis and reduce β-amyloid generation. To advance Allo into studies for chronic human neurological conditions, we examined translational and clinical parameters: dose, regimen, route, formulation, outcome measures, and safety regulations. A treatment regimen of once per week at sub-sedative doses of Allo was optimal for regeneration and reduction in Alzheimer’s pathology. This regimen had a high safety profile following chronic exposure in aged normal and Alzheimer’s mice. Formulation of Allo for multiple routes of administration has been developed for both preclinical and clinical testing. Preclinical evidence for therapeutic efficacy of Allo spans multiple neurological diseases including Alzheimer’s, Parkinson’s, multiple sclerosis, Niemann-Pick, diabetic neuropathy, status epilepticus, and traumatic brain injury. To successfully translate Allo as a therapeutic for multiple neurological disorders, it will be necessary to tailor dose and regimen to the targeted therapeutic mechanisms and disease etiology. Treatment paradigms conducted in accelerated disease models in young animals have a low probability of successful translation to chronic diseases in adult and aged humans. Gender, genetic risks, stage and burden of disease are critical determinants of efficacy. This review focuses on recent advances in development of Allo for Alzheimer’s disease (AD) that have the potential to accelerate therapeutic translation for multiple unmet neurological needs.
Innate immune mechanisms dominate elimination of adenoviral vectors following in vivo administration.

To evaluate the contribution of the innate immune component of host defense in clearing the genome of adenovirus (Ad) vectors following in vivo administration, the Ad vectors AdCMV.beta gal (expressing beta-galactosidase) or AdCMV.Null (expressing no gene) were administered intravenously to immunocompetent or immunodeficient mice, and the amount of vector genome was quantified in the liver. Strikingly, 90% of vector DNA was eliminated within 24 hr. There was no increase in vector DNA in other tissues over this period, suggesting that rapid clearance of vector genome resulted from local degradation. After 24 hr, vector elimination was slow, with only 9% of the initial amount of vector genome cleared over the subsequent 3 weeks. Importantly, early phase (0-24 hr) elimination of vector DNA was independent of the transgene and similar in immunocompetent and nude animals. These observations suggest two phases of Ad vector elimination: a previously recognized late, immune-related elimination, and the early, innate immune elimination described in the present study. The early phase of vector loss is, by far, the dominant mechanism, an observation that has implications in developing strategies to maintain persistent expression of the newly transferred gene following in vivo gene therapy.
Can the everyday technology use questionnaire predict overall functional level among older adults with mild cognitive impairment or mild‐stage alzheimer's disease? – a pilot study

INTRODUCTION The number of older adults living with mild cognitive impairment (MCI) or mild-stage Alzheimer's disease (AD) is increasing and they are often expected to live in their own homes without support, despite limited ability to perform daily life activities. The Everyday Technology Use Questionnaire (ETUQ) has proven to be able to separate these groups and might also have potential to predict overall functional level (need of assistance in daily life activities) among them. AIM To investigate whether the ETUQ can predict overall functional level among older adults with MCI or mild-stage AD. METHOD Participants were older adults with a mean age of 76 years with MCI (n = 28) or mild-stage AD (n = 39). A three-step scale indicating (i) independence, (ii) need for minimal assistance or (iii) need for moderate to maximal assistance in daily life was dichotomised in two ways and used as outcome variables in two logistic regression models. Predictors in both models were perceived ability to use everyday technology (ET) and amount of relevant everyday technologies measured by the ETUQ. Ethical approval was obtained from the regional Ethical Committee. RESULT Perceived ability to use ET discriminated individuals who were independent or in need of minimal support from those in need of moderate to maximal assistance (OR = 1.82, p < 0.01, confidence interval = 95%; 1.76-2.82). The amount of relevant everyday technologies discriminated individuals who were independent from those in need of assistance at any level (OR = 1.39; p < 0.01; confidence interval = 95%; 1.11-1.75). CONCLUSION Both perceived ability to use ET and amount of relevant everyday technologies had potential to predict overall function but at different levels. The findings support the predictive validity of the ETUQ and suggest further research for the development of clinical cut-off criteria.
Metabolic activity induces membrane phase separation in endoplasmic reticulum

Significance Membranes can adopt distinct phases. The endoplasmic reticulum (ER) is the largest membrane system inside cells and also harbors the richest metabolic activity including lipid synthesis. Unlike plasma membrane where separated “lipid raft” domains have been predicted and observed, ER membrane is thought to be uniformly fluidic. However, such understanding is based on biophysical studies of model membrane under thermodynamic equilibrium. It remains unclear whether and how lipid synthesis activity perturbs the equilibrium and promotes phase segregation in ER membrane. Herein, we utilized coherent Raman imaging technique to track lipid synthesis and surprisingly revealed solid-like domains emerging from liquid ER membrane. Interestingly, this phenomenon can be tuned by the incoming nutrient source, demonstrating the susceptibility of ER membrane to nonequilibrium modulation. Membrane phase behavior has been well characterized in model membranes in vitro under thermodynamic equilibrium state. However, the widely observed differences between biological membranes and their in vitro counterparts are placing more emphasis on nonequilibrium factors, including influx and efflux of lipid molecules. The endoplasmic reticulum (ER) is the largest cellular membrane system and also the most metabolically active organelle responsible for lipid synthesis. However, how the nonequilibrium metabolic activity modulates ER membrane phase has not been investigated. Here, we studied the phase behavior of functional ER in the context of lipid metabolism. Utilizing advanced vibrational imaging technique, that is, stimulated Raman scattering microscopy, we discovered that metabolism of palmitate, a prevalent saturated fatty acid (SFA), could drive solid-like domain separation from the presumably uniformly fluidic ER membrane, a previously unknown phenomenon. The potential of various fatty acids to induce solid phase can be predicted by the transition temperatures of their major metabolites. Interplay between saturated and unsaturated fatty acids is also observed. Hence, our study sheds light on cellular membrane biophysics by underscoring the nonequilibrium metabolic status of living cell.
The fluid software metadata framework (FSM)

The Fluid Software Metadata (FSM) framework is a dynamic and flexible framework for software repository metadata generation and analysis. FSM aims to improve the setup time for the mining, analysis, and interactive visualisation of repository artefacts. FSM supports the integration of usability findings through XML-based usability reports. Viewing usability issues side-by-side with source code provides several advantages in holistic software development.