Miracle-dz/UniFAR-FTUs huggingface dataset:
Neural correlates and predictors of speech and language development in infants at elevated likelihood for autism: a systematic review

Autism spectrum disorder (ASD) is an increasingly prevalent and heterogeneous neurodevelopmental condition, characterized by social communicative differences, and a combination of repetitive behaviors, focused interests, and sensory sensitivities. Early speech and language delays are characteristic of young autistic children and are one of the first concerns reported by parents; often before their child’s second birthday. Elucidating the neural mechanisms underlying these delays has the potential to improve early detection and intervention efforts. To fill this gap, this systematic review aimed to synthesize evidence on early neurobiological correlates and predictors of speech and language development across different neuroimaging modalities in infants with and without a family history of autism [at an elevated (EL infants) and low likelihood (LL infants) for developing autism, respectively]. A comprehensive, systematic review identified 24 peer-reviewed articles published between 2012 and 2023, utilizing structural magnetic resonance imaging (MRI; n = 2), functional MRI (fMRI; n = 4), functional near-infrared spectroscopy (fNIRS; n = 4), and electroencephalography (EEG; n = 14). Three main themes in results emerged: compared to LL infants, EL infants exhibited (1) atypical language-related neural lateralization; (2) alterations in structural and functional connectivity; and (3) mixed profiles of neural sensitivity to speech and non-speech stimuli, with some differences detected as early as 6 weeks of age. These findings suggest that neuroimaging techniques may be sensitive to early indicators of speech and language delays well before overt behavioral delays emerge. Future research should aim to harmonize experimental paradigms both within and across neuroimaging modalities and additionally address the feasibility, acceptability, and scalability of implementing such methodologies in non-academic, community-based settings.
Analysis of Airborne Fungal Communities on Pedestrian Bridges in Urban Environments

Airborne fungal spores constitute an important type of bioaerosol and are responsible for a number of negative effects on human health, including respiratory diseases and allergies. We investigated the diversity and concentration of culturable airborne fungi on pedestrian bridges in Tianjin, China, using an HAS-100B air sampler. We compared the airborne fungal communities at the top central area of the selected pedestrian bridges and along the corresponding sidewalk, at ground level. A total of 228 fungal strains belonging to 96 species and 58 genera of Ascomycota (68.86%), Basidiomycota (30.26%), and Mucoromycota (0.88%) were isolated and identified using morphological and molecular analysis. Alternaria was the dominant genus (20.61%), followed by Cladosporium (11.48%), Schizophyllum (6.14%), Sporobolomyces (5.70%), and Sporidiobolus (4.82%). Alternaria alternata was the most frequently occurring fungal species (6.58%), followed by Schizophyllum commune (5.26%), Alternaria sp. (4.82%), Sporobolomyces carnicolor (4.39%), and Cladosporium cladosporioides (3.95%). The recorded fungal concentration ranged from 10 to 180 CFU/m3. Although there was no significant difference in the distribution and abundance of the dominant airborne fungal taxa between the two investigated bridges’ sites, numerous species detected with a low percentage of abundance belonging to well-known pathogenic fungal genera, including Alternaria, Aspergillus, Aureobasidium, Cladosporium, Penicillium, and Trichoderma, were exclusively present in one of the two sites. The relative humidity showed a stronger influence compared to the temperature on the diversity and concentration of airborne fungi in the investigated sites. Our results may provide valuable information for air quality monitoring and for assessing human health risks associated with microbial pollution.
Best Practices on the Integration of Usability at the Organizational Level: A Review

—This article analyzes existing practices or guides in the literature for software organizations to improve the usability of their applications. The selected research method is the synthesis of qualitative data through a thematic synthesis. In this research, different recommendations were recovered to achieve the incorporation of usability at the organizational level, mainly derived from case studies. As a result, 13 topics grouped into 5 higher order topics have been obtained. These topics summarize the important and necessary aspects, to not only achieve usable software projects as a result, but to that usability is present as part of the essence of the organization. One of the main contributions of this work is the model of interrelation between the themes identified in this work. This model presents the existing knowledge in the literature on good practices to integrate usability at the corporate level.
Serpientes del sitio arqueológico Cueva Tixi (Pleistoceno tardío-Holoceno), provincia de Buenos Aires, Argentina

The snakes from the Cueva Tixi archeologic site (Late Pleistocene-Holocene) in the Sierras de Tandilia area, are described. The remains consist mainly in precaudal isolated vertebrae. The colubrids Clelia rustica (Cope) and Philodryas patagoniensis (Girard) and the viperid Bothrops aliernaius (Dumeril et al.) are identified; the latter species is the most abundant. The three species are common in the Sierras de Tandilia area at presento C/elia rustica and P. patagoniensis are recorded from the middle Holocene onwards, and B. alternatus since the late Pleistocene.
The demonstration of integrated coupled DFB lasers to generate tunable microwave signal

The high-frequency and tunable microwave signals are highly desirable in the files of military and civilian. The microwave photonic technology is an important solution to generate high-frequency and tunable microwave signals. Among them, the integrated microwave photonic solutions have a broader application prospect due to its small size, large bandwidth, and low power consumption, etc. We demonstrate a design of the monolithic integrated coupled DFB lasers (IC-DFB) to generate tunable microwave signal. A semiconductor optical amplifier (SOA) is integrated between DFB lasers to adjust the coupling strength. By tuning the injection current of the SOA section, microwave signals with a tuning range from 31 GHz to 35 GHz is achieve.
Development of an Advanced-Generation Multi-Objective Breeding Population for the 4th Cycle of Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook.)

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook. ) is an important timber species native to southern China. While the single, unstructured breeding strategy was employed in the past three cycles of breeding, it is no longer adequate for managing a more advanced breeding population. In this study, we utilized restriction-site-associated DNA-sequencing (RAD-seq) to estimate the genetic diversity of breeding populations and phenotypic values or breeding values to estimate the genetic gain of hundred-grain weight, diameter at breast height, and wood basic density. To achieve a balance between genetic gain and genetic diversity, we combined the multiple populations and core-main populations methods to construct the fourth cycle breeding population. Finally, the fourth cycle breeding population was made up of a core population of 50 individuals with an inbreeding coefficient of ~0, and an additional main population of 183 individuals, with an effective population size of 108. Crossings made within and/or between different trait-targeted subpopulations could facilitate bidirectional gene flow between the core and main populations, depending on the breeding objectives. This structured breeding population of Chinese fir could aim for both short- and long-term genetic gains and has the potential to support the preservation of germplasm resources for future climate change.
New Tracer for Monitoring Dynamics of Sediment Transport in Turbulent Flows

We have developed and tested a new instrumented tracer for advanced sediment transport studies, called SPY-Cobble, where the acronym SPY stands for “Single Particle dYnamics”. This new type of tracers with three internal acceleration sensors is an instrumented spherical cobble of 99 mm in diameter and a mass of 994.6 g. It was developed for detection and measurements of different elements of kinematics and dynamics (especially contact impact and friction forces) when moving at river bottom together with bedload or when resting on riverbed bottom surface.
Dark Tourism and Crime

Introduction: Encountering the remnants and reminders of crime through dark tourism 1. Auschwitz Dark Tourism 2. Oradour-sur-Glane Dark Tourism 3. Cambodia Dark Tourism 4. Argentina Dark Tourism 5. Chile Dark Tourism 6. Tasmania Dark Tourism 7. New York Dark Tourism Conclusion: towards a taxonomy of worth for crime-related dark tourism
A Model of iPSC-Derived Macrophages with TNFAIP3 Overexpression Reveals the Peculiarities of TNFAIP3 Protein Expression and Function in Human Macrophages

Macrophages play a crucial role in the development and control of inflammation. Understanding the mechanisms balancing macrophage inflammatory activity is important to develop new strategies for treating inflammation-related diseases. TNF-α-induced protein 3 (TNFAIP3, A20) is a negative regulator of intracellular inflammatory cascades; its deficiency induces hyper-inflammatory reactions. Whether A20 overexpression can dampen macrophage inflammatory response remains unclear. Here, we generated human-induced pluripotent stem cells with tetracycline-inducible A20 expression and differentiated them into macrophages (A20-iMacs). A20-iMacs displayed morphology, phenotype, and phagocytic activity typical of macrophages, and they displayed upregulated A20 expression in response to doxycycline. A20 overexpression dampened the A20-iMac response to TNF-α, as shown by a decreased expression of IL1B and IL6 mRNA. A dynamic analysis of A20 expression following the generation of A20-iMacs and control iMacs showed that the expression declined in iMacs and that iMacs expressed a lower molecular weight form of the A20 protein (~70 kDa) compared with less differentiated cells (~90 kDa). A low-level expression of A20 and the predominance of a low-molecular-weight A20 form were also characteristic of monocyte-derived macrophages. The study for the first time developed a model for generating macrophages with an inducible expression of a target gene and identified the peculiarities of A20 expression in macrophages that likely underlie macrophage preparedness for inflammatory reactivity. It also suggested the possibility of mitigating inflammatory macrophage responses via A20 overexpression.
Constituent Year Effects and Performance in Alpine Skiing Junior World Championships

This study examines constituent year effect (CYE) and race performance among junior alpine skiers in the World Championships. In various junior age cohorts competing together, variation in skiing performance can be expected not only due to practice load and experience but also due to inter-individual differences in physical and psychological maturation. Within a one-year cohort, this effect has been referred to as the birth month effect or the relative age effect (RAE). In cohorts with multiple age bands, the effect is termed the constituent year effect (CYE). The CYE works in principle as the RAE but can function as a magnifying lens of the development within a larger multi-year cohort. The results of the current study indicate that CYEs are present among junior alpine skier performance in the junior World Championships. The magnitude of the constituent year effect is greater in speed events (i.e., downhill and super-G) than in technical events (i.e., slalom and giant slalom), and greater among male skiers compared to female skiers. The findings are discussed in relation to previous research on relative age effects more generally and within the sport context specifically.
Effect of Lavage Solution Type on Bronchoalveolar Lavage Fluid Cytology in Clinically Healthy Horses

Simple Summary One of the most valuable methods to diagnose lower airway disorders such as equine asthma is broncho alveolar lavage (BAL), whereby a solution, usually saline, is inserted in a distal part of a lung lobe. The solution is then collected and contains cells present in the deeper airways. It is a safe procedure, but saline can cause local inflammation (neutrophilia). This study aimed to investigate if there are better solutions for the procedure, for example, less acidic ones, to avoid causing inflammation, and whether the BAL fluid retrieved from one lung lobe is representative for the whole lung. Since it is not necessary to use horses with respiratory disorders to answer these research questions, healthy horses were used. Four horses were used (using four lung lobe locations and four different solutions per horse twice with an interval of 48 h) and generated enough data to answer the questions. The findings were that the BAL composition is not affected by either the solution used or the lung lobe sampled. These findings are positive because they support keeping the procedure easy and practical. Abstract Equine bronchoalveolar lavage (BAL) is usually performed with 250–500 mL of isotonic saline at pH 5.5. The acidic pH of saline may cause an increase in airway neutrophil count 48 h after BAL. Other isotonic solutions such as Ringer’s solution, phosphate-buffered saline (PBS) or Plasma-Lyte 148® have a neutral pH of 7.4 and might be a better choice for BAL by not provoking inflammation and the influx of neutrophils into airways. BAL was performed in four healthy horses in four different lung lobes using four different solutions in a randomized crossover design. In each lobe, BAL was performed twice with a 48 h interval using 250 mL of solution. Automated total nucleated cell counts (TNCs) were recorded, and differential cell counts in lavage fluid were determined by two investigators blinded to treatments. The mean volume of BAL fluid retrieved was 51 ± 14%. The mean neutrophil percentage (%N) increased from 1.5 ± 0.9% to 14.7 ± 9.6% at 48 h (p < 0.001) but was not significantly affected by the solution used or the lung lobe sampled. In conclusion, in this study, the influx of neutrophils into airways after BAL was independent of the type of isotonic solution used and the lung lobe sampled. Saline remains an appropriate solution for BAL in horses.
The impact of automatic speech recognition technology on second language pronunciation and speaking skills of EFL learners: a mixed methods investigation

Introduction This study employed an explanatory sequential design to examine the impact of utilizing automatic speech recognition technology (ASR) with peer correction on the improvement of second language (L2) pronunciation and speaking skills among English as a Foreign Language (EFL) learners. The aim was to assess whether this approach could be an effective tool for enhancing L2 pronunciation and speaking abilities in comparison to traditional teacher-led feedback and instruction. Methods A total of 61 intermediate-level Chinese EFL learners were randomly assigned to either a control group (CG) or an experimental group (EG). The CG received conventional teacher-led feedback and instruction, while the EG used ASR technology with peer correction. Data collection involved read-aloud tasks, spontaneous conversations, and IELTS speaking tests to evaluate L2 pronunciation and speaking skills. Additionally, semi-structured interviews were conducted with a subset of the participants to explore their perceptions of the ASR technology and its impact on their language learning experience. Results The quantitative analysis of the collected data demonstrated that the EG outperformed the CG in all measures of L2 pronunciation, including accentedness and comprehensibility. Furthermore, the EG exhibited significant improvements in global speaking skill compared to the CG. The qualitative analysis of the interviews revealed that the majority of the participants in the EG found the ASR technology to be beneficial in enhancing their L2 pronunciation and speaking abilities. Discussion The results of this study suggest that the utilization of ASR technology with peer correction can be a potent approach in enhancing L2 pronunciation and speaking skills among EFL learners. The improved performance of the EG compared to the CG in pronunciation and speaking tasks demonstrates the potential of incorporating ASR technology into language learning environments. Additionally, the positive feedback from the participants in the EG underscores the value of using ASR technology as a supportive tool in language learning classrooms.
Trivinylphosphine Oxide: Synthesis, Characterization, and Polymerization Reactivity Investigated Using Single-Crystal Analysis and Density Functional Theory

Organophosphorus chemicals are versatile and important in industry. Trivinylphosphine oxide (TVPO), for example, exhibited a promising precursor as a flame-retardant additive for industrial applications. Density functional theory (DFT) simulations were used to explore the kinetic and thermodynamic chemical processes underlying the nucleophilic addition reactions of TVPO in order to better understand their polymerization mechanisms. An experimental X-ray single-crystal study of TVPO supported this work’s theory based on its computed findings. TVPO was prepared using POCl3 and VMB in a temperature-dependent reaction. TVPO, the thermodynamically favourable product, is preferentially produced at low temperatures. The endothermic anionic addition polymerization reaction between TVPO and VMB begins when the reaction temperature rises. An implicit solvation model simulated TVPO and piperazine reactions in water, whereas a hybrid model modelled VMB interactions in tetrahydrofuran. The simulations showed a pseudo-Michael addition reaction mechanism with a four-membered ring transition state. The Michael addition reaction is analogous to this process.
Uncertainty of material parameters for clay and its influence on simulation results

The paper presents how the uncertainty of material parameters of fine grained soils propagate to the results of numerical simulation. In particular, the posterior distribution describing the uncertainty of the five basic parameters of the hypoplastic model for clay is considered in two scenarios. In the first scenario, only the marginal distributions material parameters are considered independently and no correlation between them are assumed. In the second approach the complete multivariate posterior distribution is assumed in which the parameters are mutually correlated. This posterior distribution shows relatively low correlation between most of the parameter pairs with exception to the slope of primary consolidation line φ and its intercept N . The significantly higher variance of the simulation results obtained for the uncorrelated marginal distribution show that the correlations within the posterior distribution need to be considered in stochastic geotechnical simulations.
Anti-parasitic drug discovery against Babesia microti by natural compounds: an extensive computational drug design approach

Tick-borne Babesiosis is a parasitic infection caused by Babesia microti that can infect both animals and humans and may spread by tick, blood transfusions, and organ transplantation. The current therapeutic options for B. microti are limited, and drug resistance is a concern. This study proposes using computational drug design approaches to find and design an effective drug against B. microti. The study investigated the potentiality of nine natural compounds against the pathogenic human B. microti parasite and identified Vasicinone and Evodiamine as the most promising drugs. The ligand structures were optimized using density functional theory, molecular docking, molecular dynamics simulations, quantum mechanics such as HOMO–LUMO, drug-likeness and theoretical absorption, distribution, metabolism, excretion, and toxicity (ADMET), and pharmacokinetics characteristics performed. The results showed that Vasicinone (−8.6 kcal/mol and −7.8 kcal/mol) and Evodiamine (−8.7 kcal/mol and −8.5 kcal/mol) had the highest binding energy and anti-parasitic activity against B. microti lactate dehydrogenase and B. microti lactate dehydrogenase apo form. The strongest binding energy was reported by Vasicinone and Evodiamine; the compounds were evaluated through molecular dynamics simulation at 100 ns, and their stability when they form complexes with the targeted receptors was determined. Finally, the pkCSM web server is employed to predict the ADMET qualities of specific molecules, which can help prevent negative effects that arise from taking the treatment. The SwissADME web server is used to assess the Lipinski rule of five and drug-likeness properties including topological polar surface area and bioavailability. The Lipinski rule is used to estimate significant drug-likeness. The theoretical pharmacokinetics analysis and drug-likeness of the selected compounds are confirmed to be accepted by the Lipinski rule and have better ADMET features. Thus, to confirm their experimental value, these mentioned molecules should be suggested to carry out in wet lab, pre-clinical, and clinical levels.
Development of a High-Quality/Yield Long-Read Sequencing-Adaptable DNA Extraction Method for Crop Seeds

Genome sequencing is important for discovering critical genes in crops and improving crop breeding efficiency. Generally, fresh, young leaves are used for DNA extraction from plants. However, seeds, the storage form, are more efficient because they do not require cultivation and can be ground at room temperature. Yet, only a few DNA extraction kits or methods suitable for seeds have been developed to date. In this study, we introduced an improved (IMP) Boom method that is relatively low-cost, simple to operate, and yields high-quality DNA that can withstand long-read sequencing. The method successfully extracted approximately 8 µg of DNA per gram of seed weight from soybean seeds at an average concentration of 48.3 ng/µL, approximately 40-fold higher than that extracted from seeds using a common extraction method kit. The A260/280 and A260/230 values of the DNA were 1.90 and 2.43, respectively, which exceeded the respective quality thresholds of 1.8 and 2.0. The DNA also had a DNA integrity number value (indicating the degree of DNA degradation) of 8.1, higher than that obtained using the kit and cetyltrimethylammonium bromide methods. Furthermore, the DNA showed a read length N50 of 20.96 kbp and a maximum read length of 127.8 kbp upon long-read sequencing using the Oxford Nanopore sequencer, with both values being higher than those obtained using the other methods. DNA extracted from seeds using the IMP Boom method showed an increase in the percentage of the nuclear genome with a decrease in the relative ratio of chloroplast DNA. These results suggested that the proposed IMP Boom method can extract high-quality and high-concentration DNA that can be used for long-read sequencing, which cannot be achieved from plant seeds using other conventional DNA extraction methods. The IMP Boom method could also be adapted to crop seeds other than soybeans, such as pea, okra, maize, and sunflower. This improved method is expected to improve the efficiency of various crop-breeding operations, including seed variety determination, testing of genetically modified seeds, and marker-assisted selection.