Miracle-dz/UniFAR-FTUs huggingface dataset:
The Evaluation of the Grade of Leaf Disease in Apple Trees Based on PCA-Logistic Regression Analysis

Extensive research suggested that the core of how to use pesticides scientifically is the careful and accurate determination of the severity of crop diseases. The existing grading standards of plant leaf diseases have been excessively singular. Thus, the diseases roughly fall into general and severe grades. To address the above problems, this study considered the effect of the distribution of disease spots, and two evaluation indicators (termed the imbalance degree and main vein distance) were newly added to optimize the grading criteria of apple leaf diseases. Combined with other factors, the grade evaluation indicator was determined through PCA principal component analysis. A gradual multivariate logistic regression algorithm was proposed to evaluate apple leaf disease grade and an optimized apple leaf disease grade evaluation model was built through PCA-logistic regression analysis. In addition, three common apple leaf diseases with a total of 4500 pictures (i.e., black rot, scab, and rust) were selected from several open-source datasets as the subjects of this paper. The object detection algorithm was then used to verify the effectiveness of the new model. As indicated by the results, it can be seen from the loss curve that the loss rate reaches a stable range of around 70 at the epoch. Compared with Faster R-CNN, the average accuracy of Mask R-CNN for the type and grade recognition of apple leaf disease was optimized by 4.91%, and the average recall rate was increased by 5.19%. The average accuracy of the optimized apple leaf disease grade evaluation model was 90.12%, marking an overall increase of 20.48%. Thus, the effectiveness of the new model was confirmed.
Extended Sentinel Monitoring of Helicoverpa zea Resistance to Cry and Vip3Aa Toxins in Bt Sweet Corn: Assessing Changes in Phenotypic and Allele Frequencies of Resistance

Simple Summary Corn and cotton that produce insecticidal toxins derived from Bacillus thuringiensis (Bt) are widely adopted in the United States to control corn earworm/cotton bollworm, Helicoverpa zea (Boddie), which has resulted in major benefits to growers and the general public. However, resistance evolution in H. zea populations has become a major threat to the sustainability of these crops. Bt sweet corn producing the same toxins as Bt field corn is more attractive to H. zea than field corn and, thus, can function as a sentinel plant to detect early stages of resistance. As part of an existing sentinel monitoring network, this study evaluated changes in H. zea resistance during 2020–2022 by estimating the phenotypic and resistance allele frequencies for toxins in sentinel Bt corn. Abstract Transgenic corn and cotton that produce Cry and Vip3Aa toxins derived from Bacillus thuringiensis (Bt) are widely planted in the United States to control lepidopteran pests. The sustainability of these Bt crops is threatened because the corn earworm/bollworm, Helicoverpa zea (Boddie), is evolving a resistance to these toxins. Using Bt sweet corn as a sentinel plant to monitor the evolution of resistance, collaborators established 146 trials in twenty-five states and five Canadian provinces during 2020–2022. The study evaluated overall changes in the phenotypic frequency of resistance (the ratio of larval densities in Bt ears relative to densities in non-Bt ears) in H. zea populations and the range of resistance allele frequencies for Cry1Ab and Vip3Aa. The results revealed a widespread resistance to Cry1Ab, Cry2Ab2, and Cry1A.105 Cry toxins, with higher numbers of larvae surviving in Bt ears than in non-Bt ears at many trial locations. Depending on assumptions about the inheritance of resistance, allele frequencies for Cry1Ab ranged from 0.465 (dominant resistance) to 0.995 (recessive resistance). Although Vip3Aa provided high control efficacy against H. zea, the results show a notable increase in ear damage and a number of surviving older larvae, particularly at southern locations. Assuming recessive resistance, the estimated resistance allele frequencies for Vip3Aa ranged from 0.115 in the Gulf states to 0.032 at more northern locations. These findings indicate that better resistance management practices are urgently needed to sustain efficacy the of corn and cotton that produce Vip3Aa.
Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice

Systemic lupus erythematosus (SLE) is a chronic immune-inflammatory disease characterized by multiorgan affectation and lowered self-tolerance. Additionally, epigenetic changes have been described as playing a pivotal role in SLE. This work aims to assess the effects of oleacein (OLA), one of the main extra virgin olive oil secoiridoids, when used to supplement the diet of a murine pristane-induced SLE model. In the study, 12-week-old female BALB/c mice were injected with pristane and fed with an OLA-enriched diet (0.01 % (w/w)) for 24 weeks. The presence of immune complexes was evaluated by immunohistochemistry and immunofluorescence. Endothelial dysfunction was studied in thoracic aortas. Signaling pathways and oxidative-inflammatory-related mediators were evaluated by Western blotting. Moreover, we studied epigenetic changes such as DNA methyltransferase (DNMT-1) and micro(mi)RNAs expression in renal tissue. Nutritional treatment with OLA reduced the deposition of immune complexes, ameliorating kidney damage. These protective effects could be related to the modulation of mitogen-activated protein kinases, the Janus kinase/signal transducer and transcription activator of transcription, nuclear factor kappa, nuclear-factor-erythroid-2-related factor 2, inflammasome signaling pathways, and the regulation of miRNAs (miRNA-126, miRNA-146a, miRNA-24-3p, and miRNA-123) and DNMT-1 expression. Moreover, the OLA-enriched diet normalized endothelial nitric oxide synthase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-1 overexpression. These preliminary results suggest that an OLA-supplemented diet could constitute a new alternative nutraceutical therapy in the management of SLE, supporting this compound as a novel epigenetic modulator of the immunoinflammatory response.
Effects of High Intensity Ultrasound Stimulation on the Germination Performance of Caper Seeds

The caper bush has developed different mechanisms to survive in Mediterranean conditions, such as drought tolerance and seed dormancy. Many studies have been carried out to improve the germination of caper seeds, but ultrasound is one of the least studied methodologies in this species. This study aimed to analyze the effects of treatments with an ultrasonic probe processor on the imbibition and germination of caper seeds. After applying the ultrasound treatment using three output powers and three holding times, the seed coat’s disruption level was determined, and the imbibition, viability and germination tests were carried out. Ultrasonication fastens the initial imbibition, but after 48 h of soaking, seed moisture does not present differences compared to non-sonicated seeds. It produces the scarification of the testa but does not affect the tegmen, so moistening occurs through the hilar region, as in control seeds. There is a significant linear and negative correlation between the germination of the seeds and the temperature reached during the sonication treatment, so that temperatures above 40 °C practically annulled the germination. The combination of 20 W and 60 s provided the greatest germination percentage, being the only treatment that statistically improves germination in relation to the control seeds. When the output power and/or holding time were higher, the temperature increased, and the germination percentage statistically decreased.
Evaluation of Longitudinal Equivalent Bending Stiffness of Shield Tunnel with Residual Jacking Force

Existing tunnels are often subject to longitudinal bending when undercrossing tunnelling. It is of significance to more accurately evaluate the longitudinal equivalent bending stiffness (LEBS) of the existing tunnel within the influential zone. A new analytical method is proposed for the LEBS of tunnel segmental lining joints with consideration of incorporating combined action of residual jacking force and bending moment. The solution can degenerate into a special case with no residual jacking force, which agrees well with other classical solutions and validates the model and solutions. Sensitivity analyses are carried out for the bending moment, tunnel geometry, tensile stiffness of bolts and concrete grade on the LEBS, and effective ratio of the LEBS considering residual jacking force. The LEBS and the effective ratio of LEBS increase nonlinearly as an S-curve with the residual jacking force and decrease with an increasing bending moment. The results show that the LEBS of the shield tunnel is variable stiffness, which exhibits a significant nonlinearity. The maximum increment of the LEBS reaches 80.3% as the ring width increases from 1 m to 2 m, and the LEBS of the shield tunnel increases by approximately 1.3 × 107 kN·m2 for every 4-bolt added. The influential order on the LEBS of shield tunnels is the tunnel diameter > lining thickness > bolts diameter > ring width > the number of bolts > elastic modulus of bolts. When the effective ratio of LEBS is more than 0.85, it does not change with the ring width, lining thickness, tensile stiffness of bolts, and concrete grade. The response characteristics of the tunnel parameters on the LEBS, considering the residual jacking force, could provide a theoretical basis for the design and deformation control of shield tunnels when undercrossing tunnelling.
Single-Bilayer Graphene Test Structures for Kelvin Probe Microscopy

A new technique for determining the point spread function, which is required for measuring the surface potential using Kelvin probe microscopy (KPM), is presented. The method involves using a silicon carbide substrate coated with single-layer and bilayer graphene as a test structure and obtaining KPM potential profiles in different directions on the surface. This makes it possible to determine the KPM point spread function, which can be used to perform deconvolution and accurately recover the surface potential.
A generalization of the positive-definite and skew-Hermitiansplitting iteration

In this paper, a generalization of the positive-definite and skew-Hermitian splitting (GPSS) iteration is considered for solving non-Hermitian and positive definite systems of linear equations. Theoretical analysis shows that the GPSS method converges unconditionally to the exact solution of the linear system, with the upper bound of its convergence factor dependent only on the spectrum of the positive-definite splitting matrices. In some situations, this new scheme can outperform the Hermitian and skew-Hermitian splitting (HSS) method, the positive-definite and skew-Hermitian splitting (PSS) method, and the generalized HSS method (GHSS) and can be used as an efficient preconditioner. Numerical experiments using discretization of convection-diffusion-reaction equations demonstrate the effectiveness of the new method.
Adaptive Constant-Current/Constant-Voltage Charging of a Battery Cell Based on Cell Open-Circuit Voltage Estimation

This paper presents the novel design of a constant-current/constant-voltage charging control strategy for a battery cell. The proposed control system represents an extension of the conventional constant-current/constant-voltage charging based on the so-called cascade control system arrangement with the adaptation of the battery charging current based on the open-circuit voltage parameter estimation. The proposed control strategy features two feedback controllers of the proportional-integral type responsible for: (i) controlling the battery open-circuit voltage towards its fully charged state, and (ii) simultaneously limiting the battery terminal voltage to avoid the battery terminal voltage constraint violation. The open-circuit voltage on-line estimation is implemented by using the system reference adaptive model approach to estimate the linear time-invariant battery equivalent circuit model parameters, whose asymptotic convergence is guaranteed according to Lyapunov stability theory. The proposed concept of the battery charging control is verified by means of simulations using the experimentally obtained model of a lithium iron phosphate battery cell, and it is also compared to other charging methods with respect to charging speed-up potential compared to conventional charging. The proposed method, which can be easily extended to conventional chargers, results in 23.9% faster charging compared to conventional charging, thus representing an inexpensive and straightforward upgrade to conventional battery charging systems.
High PD-L1 Expression Correlates with an Immunosuppressive Tumour Immune Microenvironment and Worse Prognosis in ALK-Rearranged Non-Small Cell Lung Cancer

High tumour programmed cell death-ligand 1 (PD-L1) expression is associated with poor progression-free survival (PFS) after tyrosine kinase inhibitor (TKI) therapy in ALK-rearranged non-small cell lung cancer (NSCLC). However, the characteristics of the tumour microenvironment (TME) and their prognostic values in ALK-rearranged NSCLC are unknown. Here, we collected tumour tissues from pretreated ALK-rearranged NSCLC patients, immunohistochemical staining was used to assess PD-L1 expression, and tumour-infiltrating immune cells were determined via multiplex immunofluorescence staining (mIF). Our data showed that the median values of PFS for the high PD-L1 group and low PD-L1 group who received ALK-TKI treatment were 4.4 and 16.4 months, respectively (p = 0.008). The median overall survival (OS) of the two groups was 24.0 months and not reached, respectively (p = 0.021). Via univariate and multivariate analyses, a high PD-L1 expression and a worse ECOG PS were determined to be independent prognostic factors of OS (HR = 3.35, 95% CI: 1.23–9.11, p = 0.018; HR = 6.42, 95% CI: 1.45–28.44, p = 0.014, respectively). In addition, the high PD-L1 group had increased Tregs and exhausted CD8+ T cells in both the tumour and stroma (all p < 0.05). High PD-L1 expression was an adverse predictive and prognostic biomarker for ALK-rearranged NSCLC. The characteristics of the TME in patients with high PD-L1 expression were shown to have an immunosuppressive status.
Design and Performance Analysis of a Novel Integrated Solar Combined Cycle (ISCC) with a Supercritical CO2 Bottom Cycle

The integrated solar combined cycle (ISCC) system is a proven solution for grid-connected power generation from solar energy. How to further improve the ISCC system efficiency and propose a more efficient system solution has become a research focus. A novel gas turbine combined cycle (GTCC) benchmark system is proposed by replacing the conventional steam Rankine bottom cycle with a supercritical CO2 Brayton cycle, whose output power and efficiency are increased by 9.07 MW and 1.3%, respectively, compared to those of the conventional GTCC system. Furthermore, the novel ISCC systems are established with the parabolic trough solar collector (PTC) and the solar tower (ST) collector coupled to the novel GTCC system. Thermal performance analysis, exergy performance analysis, and the sensitivity analysis of the ISCC systems have been performed, and the results show that the system efficiencies of both ISCC systems are lower than that of the GTCC system, at 57.1% and 57.5%, respectively, but the power generation of the ISCC system with PTC is greater than that of the benchmark system, while that of the ISCC system with ST is less than that of the benchmark system. The photoelectric efficiency of the ISCC system with PTC is 27.6%, which is 2.1% greater than that of ISCC system with ST. In the ISCC system with PTC, the components with the highest exergy destruction and the lowest exergy efficiency are the combustion chamber, and PTC, respectively. ST is the component with the highest exergy destruction and the lowest exergy efficiency in the ISCC system with ST. With the increase in direct normal irradiance (DNI), the total output power, solar energy output power, and photoelectric efficiency of the ISCC system with PTC increase, while the system efficiency decreases; the solar energy output power and photoelectric efficiency of the ISCC system with ST increase, while the total output power and system efficiency decrease. The photoelectric efficiency of the ISCC system with PTC is greater when the DNI is greater than 600 W/m2; conversely, the photoelectric efficiency of the ISCC system with ST is greater. After sensitivity analysis, the optimal intercooler pressure for the ISCC system is 11.3 MPa.
Youth Entrepreneurship in Germany: Empirical Evidence on the How, the Why, the How Many, the Who and the When

Youth entrepreneurship is an increasingly prominent aspect of entrepreneurship support policies, but there is surprisingly little relevant research-based empirical evidence. This research gap is particularly noticeable when it comes to the personal and contextual factors that steer young people’s decision to start a business. Using statistically representative survey data from the Global Entrepreneurship Monitor for Germany, we apply logit regressions to determine the influence of 10 independent variables on the likelihood of starting a business. We distinguish between 18–24-year-olds and 25–64-year-olds as well as between founders and non-founders. Self-efficacy in entrepreneurial skills, fear of failure and gender are the strongest influencing variables for the person-related factors and knowledge of other founders for the contextual factors. For younger people, the formal level of education and the perception of local entrepreneurial opportunities do not play a role in the decision to start a business, whereas they are very important for older people. Our results suggest that start-up promotion policies should explicitly address the empirically proven factors of youth entrepreneurship instead of a ‘one size fits all’ policy for new businesses, regardless of the age of the founders.
Accuracy Assessment of Eleven Medium Resolution Global and Regional Land Cover Land Use Products: A Case Study over the Conterminous United States

Land cover land use (LCLU) products provide essential information for numerous environmental and human studies. Here, we assess the accuracy of eleven global and regional products over the conterminous U.S. using 25,000 high-confidence randomly distributed samples. Results show that in general, the National Land Cover Database (NLCD) and the Land Change Monitoring, Assessment and Projection (LCMAP) outperform other multi-class products, both in terms of higher individual class accuracy and with accuracy variability across classes. More specifically, F1 accuracy comparisons between the best performing USGS and non-USGS products indicate: (i) similar performance for the water class, (ii) USGS product outperformance in the developed (+1.3%), grass/shrub (+3.2%) and tree cover (+4.2%) classes, and (iii) non-USGS product (WorldCover) gains in the cropland (+5.1%) class. The NLCD and LCMAP also outperformed specialized single-class products, such as the Hansen Global Forest Change, the Cropland Data Layer and the Global Artificial Impervious Areas, while offering comparable results to the Global Surface Water Dynamics product. Spatial visualizations also allowed accuracy comparisons across different geographic areas. In general, the NLCD and LCMAP have disagreements mainly in the middle and southeastern part of conterminous U.S. while Esri, WorldCover and Dynamic World have most errors in the western U.S. Comparisons were also undertaken on a subset of the reference data, called spatial edge samples, that identifies samples surrounded by neighboring samples of different class labels, thus excluding easy-to-classify homogenous areas. There, the WorldCover product offers higher accuracies for the highly dynamic grass/shrub (+4.4%) and cropland (+8.1%) classes when compared to the NLCD and LCMAP products. An important conclusion while looking at these challenging samples is that except for the tree class (78%), the best performing products per class range in accuracy between 55% and 70%, which suggests that there is substantial room for improvement.
Association of Coffee and Tea Intake with Bone Mineral Density and Hip Fracture: A Meta-Analysis

Background and Objectives: Osteoporosis is characterized by low bone mass and high bone fragility. Findings regarding the association of coffee and tea intake with osteoporosis have been inconsistent. We conducted this meta-analysis to investigate whether coffee and tea intake is associated with low bone mineral density (BMD) and high hip fracture risk. Materials and Methods: PubMed, MEDLINE, and Embase were searched for relevant studies published before 2022. Studies on the effects of coffee/tea intake on hip fracture/BMD were included in our meta-analysis, whereas those focusing on specific disease groups and those with no relevant coffee/tea intake data were excluded. We assessed mean difference (MD; for BMD) and pooled hazard ratio (HR; for hip fracture) values with 95% confidence interval (CI) values. The cohort was divided into high- and low-intake groups considering the thresholds of 1 and 2 cups/day for tea and coffee, respectively. Results: Our meta-analysis included 20 studies comprising 508,312 individuals. The pooled MD was 0.020 for coffee (95% CI, −0.003 to 0.044) and 0.039 for tea (95% CI, −0.012 to 0.09), whereas the pooled HR was 1.008 for coffee (95% CI, 0.760 to 1.337) and 0.93 for tea (95% CI, 0.84 to 1.03). Conclusions: Our meta-analysis results suggest that daily coffee or tea consumption is not associated with BMD or hip fracture risk.
Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

The prevalence of Listeria monocytogenes in bovine bulk-tank milk (BTM) in Greece has not been previously investigated. The aim of the study was to estimate the prevalence of L. monocytogenes in bovine BTM in Greece and to characterize the isolates in terms of carriage of genes encoding for pathogenic determinants, assess the isolates’ biofilm-forming ability and determine their susceptibility against 12 antimicrobials. Samples (n = 138) of bovine BTM were obtained from farms located throughout Northern Greece and were analyzed qualitatively and quantitatively for L. monocytogenes. Five samples (3.6%) tested positive for L. monocytogenes. The pathogen’s populations in these positive samples were below 5 CFU/mL. Most isolates belonged to the molecular serogroup “1/2a, 3a”. All isolates carried the virulence genes inlA, inlC, inlJ, iap, plcA and hlyA, but actA was detected in only three isolates. The isolates displayed weak to moderate biofilm-forming ability and distinct antimicrobial resistance profiles. All isolates were characterized as multidrug resistant, with resistance to penicillin and clindamycin being a common feature. Considering that L. monocytogenes constitutes a serious public health threat, the key findings of the study, related to the carriage of virulence genes and multidrug resistance, highlight the importance of continued monitoring of the pathogen in farm animals.