Miracle-dz/UniFAR-FTUs huggingface dataset:
This information is current as Responses to Influenza A Virus Enhances Innate and Adaptive Immune p 53 Serves as a Host Antiviral Factor That

Several direct target genes of the p53 tumor suppressor have been identified within pathways involved in viral sensing, cytokine production, and inflammation, suggesting a potential role of p53 in antiviral immunity. The increasing need to identify immune factors to devise host-targeted therapies against pandemic influenza A virus (IAV) led us to investigate the role of endogenous wild-type p53 on the immune response to IAV. We observed that the absence of p53 resulted in delayed cytokine and antiviral gene responses in lung and bone marrow, decreased dendritic cell activation, and reduced IAV-specific CD8 + T cell immunity. Consequently , p53 2/2 mice showed a more severe IAV-induced disease compared with their wild-type counterparts. These findings establish that p53 influences the antiviral response to IAV, affecting both innate and adaptive immunity. Thus, in addition to its established functions as a tumor suppressor gene, p53 serves as an IAV host antiviral factor that might be modulated to improve anti-IAV therapy and vaccines. I nfluenza A virus (IAV) poses a global health and economic threat due to the emergence of IAV epidemics and pandemics at random intervals (1). The high rate of viral mutation (anti-genic drift) and the putative emergence of reassortant strains (an-tigenic shift) have raised the need to find host-targeted therapeutic strategies to devise antiviral treatments (2, 3). Current efforts are focused on the identification of host factors with a role in the early inflammatory responses to IAV infection, including TLRs (4), C-type lectins (5), inflammasomes (6), and chemokine receptors (7). However, a possible limitation of such strategies is the fact that these protein families are mainly expressed in the hematopoietic compartment and not in the epithelial cells of the respiratory tract, which as primary targets of the virus play a key role in the induction of early cytokine and antiviral gene responses (8). Recently, we identified a positive feedback loop involving p53-dependent enhancement of IFN signaling through transcriptional upregulation of IFN regulatory factor (IRF)9 (9). By doing so, p53 not only promotes the transactivation of IFN-stimulated genes (ISGs), but also enhances IFN production from virus-infected cells. Other reports indicate that in addition to IRF9, other genes involved in innate immunity are also p53 direct transcriptional targets, including pattern recognition receptors such as TLR3 (10), additional IRFs such as IRF5 (11, 12), antiviral genes such as ISG15 (13) and dsRNA-activated protein kinase R (14), and proinflam-matory chemokines such as MCP-1 (15). These …
Inducible Promoter-Repressor System from the Lactobacillus casei

With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage FSW-TI in Escherichia coli. The FSW-TI promoter fragment was abutted to the plasmid-borne promoterless -glucuronidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of the promoter-repressor system was verified in the L. casei FSW-TI lysogen by showing that the gusA reporter gene, controlled by the isolated FSW-TI promoter, was repressed at 28°C and expressed at 42°C. Moreover, a homology search revealed that the C terminus of the isolated FSW repressor shows a high similarity to the small mutS-related domain of the MutS2 protein family that is unprecedented for phageencoded repressor proteins. Given the economic importance of lactic acid bacteria, and more recently their prospective use as safe vaccines, a number of physiological traits as well as food-grade expression tools of lactobacilli and lactococci have been studied (9). Various inducible gene expression systems have been developed for Lactococcus lactis (reviewed in reference 3). In general, these transcriptional induction systems require the exogenous supply of inducers (18, 19, 21). In addition, a heat-inducible homologous expression system has been developed for L. lactis based on a genetically engineered thermolabile repressor of the lactococcal phage r1t (17). In lactobacilli, studies on the regulation of gene expression have focused mainly on carbon catabolism pathways, and an inducible-repressible expression system derived from the lactose operon of Lactobacillus casei has been described (5 and references therein). The temperate phage FSW-TI was isolated from L. casei strain Shirota (S1) after chemical mutagenesis of a lysogen followed by heat induction (22, 24). Since the mutation responsible for the thermoinducibility was shown to reside in the phage DNA, it seemed likely that it could affect the stability of the phage repressor at the elevated temperature. In this study, we have characterized a promoter and a cognate repressor of FSW-TI with the potential to be exploited in a thermoinducible food-grade expression system for L. casei. Isolation of a promoter and a cognate heat-labile repressor of FSW-TI in Escherichia coli. The following strategy was used to isolate the thermoinducible promoter-repressor cassette of phage FSW-TI. The FSW-TI DNA was first shotgun cloned into the E. coli promoter test vector pKK232-8 (Amersham Pharmacia) carrying a promoterless cat gene. Chloramphenicol (Cm)-resistant clones were cotransformed with a FSW-TI library generated in the compatible plasmid pK194 (7). The DNA sequence encoding the protein with apparent repressor activity and its cognate promoter sequence were then obtained from clones that were sensitive and resistant to the antibiotic at 28 and 42°C, respectively.
Sustainable Strategies for Housing the Urban Poor: A Case Study of Lagos, Nigeria

The environment in Africa is a product of the Triple heritage: the indigenous culture, western influence and Islamic legacies (Elleh, 1997). These factors combine to form cities different from any other part of the world. This uniqueness does not exclude African cities from problems facing developing countries. Squatting is a major problem in most African urban areas, where the rate of migration far exceeds states resources for public housing construction. This paper focuses on slums and squatter settlements in Lagos, Nigeria, and proposes strategies for developing affordable prototype housing using sustainable strategies, indigenous materials and skills, community based planning processes and culture-based design aesthetics. Field research conducted in two settlements in Lagos: Makoko and Agege addressed characteristics of slums and squatter settlements such as availability of drainage systems, infrastructure, electricity, water, and basic amenities. The author proposes use of locally available materials, exploration of alternative energy systems such as photovoltaic systems or wind to generate electricity, efficient lighting systems with daylighting integrated, wood and adobe construction for regional abundance and thermal properties, interior finishes with low volatile organic compounds (vocs) and water efficiency through water reduction and water efficient landscaping as sustainable strategies for housing the urban poor. Data obtained through field research helped students understand the ramifications of slums and squatter settlements in Lagos and students developed design solutions for the slums and squatter settlements in Lagos.
Fact or fiction ? : testing effects of suggested illuminance changes

• A submitted manuscript is the author’s version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher’s website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers.
Building Community: Collaborative Individualism and the Challenge for Building Social Capital

Mixed-tenure developments, such as master-planned communities and urban villages, often presuppose that the design will build community and enhance social capital, thus mitigating the negative social impacts of suburban sprawl. However, research highlights the difficulties associated with creating community within such developments, citing demographic mix and service provision as both positive and negative elements. This paper explores the extent to which social capital exists in a planned, mixed-tenure development, from the perspective of residents living in low to medium-density dwellings in the estate. The results from a quantitative study examining sustainable community development on Queensland's Gold Coast, Australia, suggest that planned mixed-tenure developments have an equal chance of providing options for demographically diverse stakeholders as any other kind of development including the traditional suburban format. Outcomes such as increased levels of social capital appear to depend upon the combination of residents and their inclinations towards individual or altruistic benefits for their families, neighbourhoods, or communities. Inevitably, it seems that no amount of planning can provide a formula that will be effective in all instances.
Effectiveness of Mindfulness-Based Cognitive Therapy (MBCT) in PreventingDepression Symptoms

Mindfulness-Based Cognitive Therapy (MBCT) is quickly gaining more popularity in the treatment of various disorders including depression. Mindfulness-Based Cognitive Therapy (MBCT) combines mindfulness meditation and cognitive techniques to alleviate depression, enhance well-being, and prevent relapse in individuals with recurrent episodes. This integrative approach fosters emotional regulation, positive mood, and potential spiritual experiences. This systematic review focused on investigating existing research on MBCT and depression. To explore the effectiveness of MBCT and its mechanisms of action that help in breaking the negative thought patterns developed in people when dealing with major depression. This study identified the large number of studies that recommend MBCT as an effective psychological intervention to reduce depression and anxiety symptoms in clinical and non-clinical populations and have also demonstrated preventing the recurrence of depression.
Subaerial deposition of mine tailings by dimensionless particle size

An existing theory of subaerial sorting and deposition on mine tailings and co-disposal beaches relies on the assumption that deposition is governed by the dimensionless particle size. This is well established for uniform particles in laboratory flumes and for natural flows with low solids concentrations, but has not been validated for mine waste beaches, where particle size and specific gravity vary significantly and solids concentrations are high. The data presented confirm that the dimensionless particle size governs deposition on a coal mine tailings beach with a moderately high solids concentration, supporting the theory. However, it remains uncertain whether the same applies for very high solids concentrations.
UvA-DARE (Digital Polyamine metabolism and activation of lipid signalling pathways in Arabidopsis thaliana

Polyamines, such as putrescine (Put), spermidine (Spd) and spermine (Spm), are low-molecular-weight polycationic molecules found in all living organisms. Despite having been implicated in various important cellular processes, their mode of action is still largely unclear. Accumulating evidence suggest that polyamines can interact with macromolecules and cellular structures, such as membranes, and affect signal transduction processes. Here, we provide evidence for an interaction between polyamines and the polyphosphoinositide-(PPI) signalling pathway, using Arabidopsis thaliana seedlings as a model system. Exogenous application of Put, Spd and Spm all led to a rapid and significant increase of the signalling lipid, phosphatidylinositol 4,5-bisphosphate (PIP 2 ). Using time-course and dose-response experiments, Spm was found to be the most effective at promoting PIP 2 responses at physiological (µM) concentrations. The increase in PIP 2 mainly occurred in the root and, at least partly, involved the plasma-membrane localised uptake transporter, RMV1. Using a differential 32 P i -labelling strategy, we found that the increase in PIP 2 was caused by the enzyme, phosphatidylinositol 4-phosphate 5-kinase (PIP5K), of which Arabidopsis is predicted to encode 11 genes. Using T-DNA insertion mutants, we identified AtPIP5K7 and AtPIP5K9 as the main candidates involved. Expressing a genetically-encoded PIP 2 -biosensor in both wild type and pip5k7/pip5k9 double-mutant backgrounds, their involvement was confirmed and revealed that the Spm-induced PIP 2 response predominantly accumulated at the plasma membrane of root cells within 30 min of treatment. The increase of PIP 2 was found to correlate with a gradual and transient K + efflux through the PM, as measured with the non-invasive microelectrode ion flux estimation (MIFE) technique in wt and pip5k7/pip5k9 -mutant roots. Our results provide biochemical and genetic evidence for an early interaction of polyamines with the PM, and identified a potential signalling route downstream, involving PIP 2 and the efflux of K + . The potential significance of these results will be discussed. mL of standard growth medium consisting of ½ Murashige and Skoog (MS) medium with Gamborg B5 vitamins (pH 5.7; KOH), 1% (w/v) sucrose, and 1% (w/v) agar. Plates were vernalized at 4 ºC for 48 h and then placed vertically, under the angle of 70º, in a growth chamber (16/8 light/dark cycle, 110-130 µ mol m -2 s -1 ) at 22 °C. Five days-old seedlings were transferred to either 2 mL tubes for 32 P i labelling experiments, or to small round petri dishes for incubations with polyamines and chemicals. Glass incubations were avoided since polyamines tend to stick to glass surfaces. For gene expression analyses, seeds were germinated on a nylon mesh (Ø 43 µm) placed on top of the medium. Mature plants were grown on soil following the same light and temperature regime as described above.
Vulnerability forecasting model based on coupling technique of GIS and ANN in floor groundwater bursting

Based on analyzing physical geography,regional geologic structure and mine-hydrogeologic conditions,and groundwater bursting characteristics in the tenth coal mine,Hebi,thematic layers of the each dominating factor for floor groundwater bursting was constructed with the spacial data statistics and analyses fuctions of the GIS,according to the spacial change situations of the groundwater pressure in the aquifer,fault density,thickness and lithology of aquifuge,karst development etc.The weight coefficients of the each dominating factor for floor groundwater bursting were determined with ANN.On the basis of application of coupling technique of GIS and ANN,established a vulnerability forecasting model of the floor groundwater bursting,and determined the divided zone threshold values with a cumulative frequency histogram of the floor groundwater bursting vulnerability index,and analyzed the sensitivity features for the each dominating factor.
COMPARATIVE STUDY OF ANTIOXIDANT ACTIVITIES OF CULTIVATED AND WILD GANODERMA LUCIDUM (W. CURT. FR.) P. KARST APHYLLOPHOROMYCETIDEAE FROM NORTH WESTERN INDIAN HIMALAYAS

Ganoderma lucidum is a high value medicinal mushroom. Many active substances with immuno-modulating effects have been isolated from G. lucidum. The fruiting bodies collected from tree trunk of Dalbergia sissoo was identified and isolated using 2.0% Malt-Extract Agar (MEA) medium and mushroom was cultivated over the substrate wheat straw. Fruiting bodies were analyzed for different phytochemicals and antioxidant activities. The findings indicated that cultivated G. lucidum contained higher phenolics (28.27 mg GAE/g dry wt.) in comparison to wild (24.21 mg GAE/g dry wt.) mushroom fruiting body. Results also showed higher radical scavenging activity on DPPH (10-81.21%) and ABTS (29.34-99.45%), FRAP (98.17-382.01), total antioxidant activity (167.24375.74), reducing power (0.10-0.40) and metal chelating activity (39.37-75.0%) in cultivated isolate. All the activities were found to be increased with increase in concentration both in cultivated and wild G. lucidum. In general cultivated isolate had higher antioxidant activities in comparison to wild one.
Dynamic Effectiveness of Advertising and Word of Mouth in Sequential Distribution of New Products

Firms in many industries release new products in sequential stages. They also launch separate advertising campaigns at each distribution stage. Thus, communication mix elements—advertising and word of mouth (WOM)—can play important, distinct, and yet interdependent roles in stimulating new product demand. Their effectiveness may fluctuate within and across stages and spill over from earlier to later stages. Thus, the authors construct a dynamic linear model to study the dynamic effects of advertising and WOM on demand for heterogeneous products across stages. They further apply the model to examine a canonical example, the theater-then-video sequential distribution of motion pictures, and estimate the parameters using Kalman filtering/smoothing and Markov chain Monte Carlo methods. The results show that advertising and WOM exert dynamic, yet diverse, influences on demand for new products. For example, while increased ad spending is more effective at an earlier stage due to repetition wear-in and synergy with WOM, increased WOM activities at a later stage could become more powerful in driving demand. Subsequent optimization exercises suggest that films of varied characteristics can potentially re-allocate their advertising budgets and reap additional revenues.
Microsoft Word-40992643-file00.docx

School of Life Science and Technology, Tongji University, Shanghai 200092, China (SR, JF, LC); Non-Clinical Safety, Roche Pharma Research and Early Development China, Shanghai 201203, China (SR); Medicinal Chemistry, Roche Pharma Research and Early Development China, Shanghai 201203, China (JZ, SFY); State Key Laboratory of Precision Spectroscopy, Department of Physics, Institute of Theoretical and Computational Science, East China Normal University, Shanghai 200062, China (JZ, YM, JZHZ); Department of Chemistry, New York University, New York, New York 10003, USA (JZHZ) DMD Fast Forward. Published on October 2, 2012 as doi:10.1124/dmd.112. 048264
CENTRAL ASIAN JOURNAL OF LITERATURE, PHILOSOPHY AND CULTURE

In this article, opinions are expressed about modern methods of teaching music lessons in secondary schools. Also, detailed information about methods and methods of organizing music lessons by pedagogues and modern interactive teaching is provided. In the scientific research, the reforms implemented in the field and its results, proposals related to the relevance of the topic are highlighted
Hydrocarbon-Expulsion Characters and the Potential Resource Evaluation of Hydrocarbon Source Rocks from the Upper Section of 4th Member of Shahejie Formation in Dongying Depression

The lithologic pool plays a more and more important role in Dongying Depression,so it is necessary to enhance study its main hydrocarbon source rocks in terms of hydrocarbon-expulsion characters and potential resource evaluation.The upper section of 4th member of Shahejie Formation includes two sets of hydrocarbon source rocks: dark mudstone and oil shale.In this paper,based on the theory of hydrocarbon-expulsion threshold,the hydrocarbon-expulsion characters and the potential resource evaluation of the upper section of 4th member of Shahejie Formation is discussed by hydrocarbon-generation potential method.The upper section of 4th member of Shahejie Formation discharged hydrocarbon mainly in the depositing stage of Minghuazheng Formation,with its hydrocarbon-expulsion threshold depth at 2 600 m.The total hydrocarbon-expulsion and the total sources are 59.67×108 t and 25.66×108t,in which the dark mudstone holds more than 72.18%,and the oil shale holds 27.82%. The comprehensice analysis leads to the conclusion that the the upper section of 4th member of Shahejie Formation is the main hydrocarbon source rocks to the lithologic pools in Dongying Depression.
Enterprise Risk Management: A process for enhanced management and improved performance

Enterprise risk management (ERM) is a new, board-supervised process that aims to identify, evaluate, and manage all major corporate risks in an integrated framework. Advocated by the Committee on Sponsoring Organizations of the Treadway Commission (COSO) in 2004, ERM adoption has been encouraged by global regulatory agencies, various stock exchanges, legal court cases, and rating agencies. Applied in a strategic setting, ERM takes a company-wide perspective and incorporates strategic and operational risks as well as the more traditional insurance, financial and legal risks. In spite of the growing importance of ERM, there has been very little research on the subject. Existing research has included field studies of companies adopting ERM, has investigated the determinants of ERM adoption, and has explained the appointment of a chief risk officer. We extend this early work by examining how ERM leads to enhanced management and improved performance. Based on a survey of 150 risk management executives who are implementing ERM and tested with the partial least squares (PLS) analytical technique, our model shows that an ERM-based framework can help a company improve performance. Our model also confirms both the linear aspect of building an ERM process and the interactive aspects, where later ERM components help companies improve on earlier processes. Interestingly, our model also shows the key reason why ERM improves performance: it first enhances management. In other words, the value side of ERM is that it makes management better (as seen in greater management consensus, better informed decision making, and increased accountability).