Miracle-dz/UniFAR-FTUs huggingface dataset:
The Tetraspanin KAI1/CD82 Is Expressed by Late-Lineage Oligodendrocyte Precursors and May Function to Restrict Precursor Migration and Promote Oligodendrocyte Differentiation and Myelination

In the adult mammalian brain, oligodendrocyte progenitors can differentiate into mature oligodendrocytes during remyelination. Mechanisms that regulate migration and differentiation of progenitors are of great importance in understanding normal development and demyelinating/remyelinating conditions. In a microarray analysis comparing adult and neonatal O4-positive (+) cells, we found that the tetraspanin KAI1/CD82 is far more highly expressed in adult O4+ cells than in neonatal O4+ cells (Lin et al., 2009). CD82 is a metastasis suppressor, and its expression is often downregulated or lost in the advanced stages of metastatic cancer. We hypothesized that CD82 could be a factor that restricts migration and promotes differentiation of maturing oligodendrocytes. Western blot analysis of isolated adult O4+ cells confirms the elevated levels of CD82, which continues to be expressed as these become O1+ in vitro. In the adult rat white matter, CD82 is coexpressed with CC1 and olig2 but not with NG2 or GFAP. Immature cells of the neonatal forebrain subventricular zone (SVZ) infected in vivo with a retrovirus that constitutively expresses CD82 do not remain immature but differentiate into either CC1+ and MBP+ myelinating oligodendrocytes in the white matter or zebrinII+ astrocytes in the cortex. Their migration from the SVZ is severely restricted. In contrast, downregulation of CD82 in SVZ cells in vivo, using retroviral-expressed short hairpin RNAs (shRNAs), prevents their differentiation into myelinating oligodendrocytes. shRNA-expressing cells remained PDGF receptor α positive, olig2+, or NG2+ or became CC1+ nonmyelinating oligodendrocytes or GFAP+ astrocytes. CD82 thus appears to be a critical molecule in the regulation of oligodendrocyte progenitor migration and myelination.
Assessment of intensity effort of middle-aged adults practicing regular walking

Background: Walking is one of the most commonly recommended activities for sedentary individuals. When performed at the correct intensity, it can provide cardiovascular, respiratory, metabolic, and other benefits by providing a training effect in addition to reducing the risk of death from cardiovascular diseases and other chronic health conditions. Objectives: The primary aim of this study was to assess whether individuals who practiced regular unsupervised walking carry out the activity safely and with sufficient effort intensity parameters to have a positive physiological (training) effect. The secondary objective was to compare the training heart rate (HR) and the stability of the HR within the ideal range of training between the sexes. Method: Individuals were selected from walking tracks within the city of Belo Horizonte, Brazil. The study included subjects from 40 to 60 years of age who had practiced walking for at least two months prior to the study, walking at least three times a week. Individuals who agreed to participate in the survey were asked to walk 15 minutes at their usual pace with their HR measured every 5 minutes using a heart rate monitor. Their average walking HR was compared to the average training HR based on the formula: (220 - age) × 70 to 80% that would result in a positive physiological training effect. Results: Of the 142 individuals evaluated, 25.4% achieved the average training HR. This result was significantly lower than those who did not achieve the average training HR while walking (p=0.002). There were significant differences between men and women who had reached the training HR (p=0.0001). Conclusion: The authors found that individuals who walk regularly performed outside the range of the ideal HR intensity that would cause a positive physiological effect and therefore would probably not achieve a beneficial training effect while walking.
Baraitser–Winter Syndrome Hotspot Mutation R196H in Cytoskeletal β–actin Reduces F–actin Stability and Perturbs Interaction with the Arp2/3 Complex

Baraitser–Winter cerebrofrontofacial syndrome (BWCFF) is the most common and best–defined clinical entity associated with heterozygous single–point missense mutations in cytoskeletal β–actin. Patients present with distinct craniofacial anomalies and neurodevelopmental disabilities of variable severity. To date, the most frequently observed variants affect residue R196 of cytoskeletal β–actin, with the variant p.R196H being the most common. Patients carrying the p.R196H variant are likely to suffer from pachygyria, probably due to neuronal migration defects contributing to the development of abnormally thick convolutions of the cerebral cortex. Here, we describe the recombinant production, purification and biochemical characterization of the BWCFF hotspot variant p.R196H. The stability and nucleotide interaction of monomeric p.R196H are unaffected, indicating a disease mechanism involving incorporation of p.R196H protomers into actin filaments. Incorporation of the variant strongly affects F–actin stability and polymerization dynamics, consistent with the position of residue R196 close to the helical axis of the actin filament and an important interstrand contact. The changes observed include an increased critical concentration of polymerization, a reduced elongation rate and an increase in the rate of filament depolymerization. In the Arp2/3–generated branch junction complex, which is essential for cell migration and endocytosis, R196 is located at the interface between the first protomer of the nucleated daughter filament and the Arp2 subunit of the Arp2/3 complex. Assays probing the interaction of p.R196H filaments with the Arp2/3 complex show a reduced efficiency of branch generation. Branch stability is impaired, as evidenced by a reduction in the number of branches and spontaneous debranching events. Furthermore, in their interaction with different types of cytoskeletal myosin motors, p.R196H filaments show isoform–specific differences. While p.R196H filaments move WT–like on lawns of surface–immobilized non–muscle myosin–2A, motility on myosin–5A is 30 % faster.
Pediatric multiple sclerosis

Purpose of review This review discusses the epidemiologic factors involved in the pathogenesis of pediatric multiple sclerosis (MS), which have been the focus of numerous studies in the last several years. We also review the clinical features (including diagnostic evaluation and differential diagnosis) of, treatment approach to, and prognosis of pediatric MS. Recent findings Up to 10% of patients with MS have their initial demyelinating before the age of 18 years. Over the past 15 years, international and collaborative studies have identified an increasing number of genetic and environmental risk factors for pediatric MS. Identification of these risks and their interplay allow for better understanding of the pathophysiology of pediatric MS, which may inform subsequent treatment and disease management. Careful attention to the management of relapses and chronic symptoms, including implementation of lifestyle modifications and pharmacologic interventions, enables improved school performance and quality of life. Summary Ongoing research in the field of pediatric MS aims to better understand the epidemiologic factors involved in the pathobiology, safety and efficacy of disease-modifying treatments, and long-term prognosis, particularly of cognitive development and academic potential. Collaborative, multinational studies will enable the advancements needed to truly optimize clinical care for this population.
COMMUNICATION REALLY IS VITAL

The interests of the patient are jeopardized whenever there is a breakdown in communication between the doctor working in the area of primary care and doctors in hospitals and in specialist practice. Deficiences are common, and occur when doctors see patients who are already under treatment from some other source, and when they refer patients for specialist advice. With patients already under treatment there may be no information available as to previous diagnosis, investigations performed and their results, or the treatment the patient has been instructed to take. This information is especially important should a crisis arise at the patient's home. Where it is lacking it is impossible for the patient to receive the optimal care.
Deblending random seismic sources via independent component analysis

We consider the question of deblending for seismic shot records generated from simultaneous random sources at different locations, i.e., how to decompose them into isolated records involving one source at a time. As an example, seismic-while-drilling experiments use active drill-string sources and receivers to look around and ahead of the borehole, but these receivers also record noise from the operation of the drill bit. A conventional method for deblending is independent component analysis (ICA), which assumes a “cocktail-party” mixing model where each receiver records a linear combination of source signals assumed to be statistically independent, and where only one source can have a Gaussian distribution. In this note, we extend the applicability of ICA to seismic shot records with markedly more complex mixing models with unknown wave kinematics, provided the following assumptions are met.
PCR confirmation of infection with Cyclospora cayetanensis.

persons and the public health relevance of this emerging pathogen as a potential cause of diarrheal outbreaks (3,4) make prompt disclosure of the epidemiologic features and behavior of the parasite necessary. As we propose the possible participation of poultry in the epidemiologic cycle of the coccidia, we invite other Cyclospora working groups worldwide to confirm the so far putative reservoir described in this communication and to further study other possible hosts or reservoirs.
Effect of vision angle on the phase transition in flocking behavior of animal groups.

The nature of the phase transition in a system of self-propelling particles has been extensively studied during the past few decades. A theoretical model was proposed by [T. Vicsek et al. Phys. Rev. Lett. 75, 1226 (1995)PRLTAO0031-900710.1103/PhysRevLett.75. 1226] with a simple rule for updating the direction of motion of each particle. Based on the model of Vicsek et al., in this paper, we consider a group of animals as particles moving freely in a two-dimensional space. Due to the fact that the viewable area of animals depends on the species, we consider the motion of each individual within an angle φ=ϕ/2 (ϕ is called the angle of view) of a circle centered at its position of radius R. We obtained a phase diagram in the space (φ,η_{c}) with η_{c} being the critical noise. We show that the phase transition exists only in the case of a wide view's angle φ≥0. 5π. The flocking of animals is a universal behavior of the species of prey but not the one of the predator. Our simulation results are in good agreement with experimental observation [C. Beccoa et al., Physica A 367, 487 (2006)PHYADX0378-437110.1016/j.physa. 2005.11. 041].
Cognitive consequences of forced compliance.

WHAT happens to a person's private opinion if he is forced to do or say something contrary to that opinion? Only recently has there been, any experimental work related to this question. Two studies reported by Janis and King (1954; 1956) clearly showed that, at least under some conditions, the private opinion changes so as to bring it into closer correspondence with the overt behavior the person was forced to perform. Specifically, they showed that if a person is forced to improvise a speech supporting a point of view with which he disagrees, his private opinion moves toward the position advocated in the speech. The observed opinion change is greater than for persons who only hear the speech or for persons who read a prepared speech with emphasis solely on elocution and manner of delivery. The authors of these two studies explain their results mainly in terms of mental rehearsal and thinking up new arguments. Inthisway, they propose, theperson who is forced to improvise a speech convinces himself. They present some evidence, which is not altogether conclusive, in support of this explanation. We will have more to say concerning this explanation in discussing the results of our experiment. Kelrnan (1953) tried to pursue the matter further. He reasoned that if the person is induced to make an overt statement contrary to his private opinion by the offer of some reward, then the greater the reward offered, the greater should be the subsequent opinion change. His data, however, did not support this idea. He found, rather, that a large reward produced less subsequent opinion change than did a smaller reward. Actually, this finding by Kelman is consistent with the theory we will outline below but, for a number of reasons, is
Can human decisions be predicted through heart rate changes?

Researchers in the area of neuroscience, psychophysiology and communication technology believe that emotion plays an important role in decision making, learning and memory. Recent years, due to the development of instrument and experimental paradigm, emotion and its' influence on decision making has become clear. This paper utilized Iowa Gambling Task (IGT) and Heart Rate Variability (HRV) analysis, found out subjects having different types of decisions related to their heart rate changes right before they made decisions (p &#60; 0.001). As a pattern classification problem, promising results were obtained in this study using Partial Regularized Least Squares (PRLS) extended feature extraction techniques, showing that human decisions are predictable through their concurrent physiological state.
Structure/function assessment of synapses at motor nerve terminals

The release of transmitter at neuromuscular junctions (NMJ) of the opener muscle in crayfish is quantal in nature. This NMJ offers the advantage of being able to record quantal events at specific visually identified release sites, thus allowing measurement of the physiological parameters of vesicle release and its response to be directly correlated with synaptic structure. These experiments take advantage of areas between the varicosities on the nerve terminal that we define as “stems. ” Stems were chosen as the region to study because of their low synaptic output due to fewer synaptic sites. Through 3D reconstruction from hundreds of serial sections, obtained by transmission electron microscopy (TEM), at a site in which focal macropatch recordings were obtained, the number of synapses and AZs are revealed. Thus, physiological profiles with various stimulation conditions can be assessed in regards to direct synaptic structure. Here, we used the properties of the quantal shape to determine if distinct subsets of quantal signatures existed and if differences in the distributions are present depending on the frequency of stimulation. Such a quantal signature could come about by parameters of area, rise time, peak amplitude, latency, and tau decay. In this study, it is shown that even at defined sites on the stem, with few active zones, synaptic transmission is still complex and the quantal responses appear to be variable even for a given synapse over time. In this study, we could not identify a quantal signature for the conditions utilized. Synapse, 2011. © 2010 Wiley‐Liss, Inc.
Rural Education Remote Special Educational Consultation in Swedish Rural Schools: Experiences of Special Educators and Class Teachers

With advances in communication technologies, remote special educational consultation has become a promising strategy for indirect special education provision aiming to support students with special educational needs as well as their teachers in remote and rural areas. This study explores how special educators in a rural municipality in Sweden offer remote special educational consultation to teachers and how the two teacher categories experience remote special educational consultation. The empirical data of the s tudy consist of special educators’ and teachers’ responses to an online questionnaire (N=11). The analysis revealed themes reflecting experienced challenges as well as opportunities. The challenges relate to lack of professional commitment and consensus, lack of school leaders’ trust, knowledge and support, and barriers associated with distance and technology, while opportunities relate to increased access to special educational competence, increased structure in consultation and increased professional development and collaboration. The results of this study highlight the potential value of remote special educational consultation as an acceptable, cost-effective, and efficient way of increasing access to special educational expertise and providing special educational support to teachers working in rural and underserved schools.
Metaphor in Discourse

Many have thought that the principles of metaphorical interpretation cannot be formally specified. We’ll attack this position with two claims. Our first claim is that some aspects of metaphor are productive, and this productivity can be captured by perspicuous links between generalisations that are specified in the lexicon, and general purpose circumscriptive reasoning in the pragmatic component. Indeed from a methodological perspective, we would claim the productive aspects of metaphor can give the lexicographer clues about how to represent semantic information in lexical entries.
Vascular endothelial growth factor/vascular permeability factor enhances vascular permeability via nitric oxide and prostacyclin.

BACKGROUND Vascular endothelial growth factor (VEGF), an endothelial cell mitogen that promotes angiogenesis, was initially identified as a vascular permeability factor (VPF). Abundant evidence suggests that angiogenesis is preceded and/or accompanied by enhanced microvascular permeability. The mechanism by which VEGF/VPF increases vascular permeability (VP), however, has remained enigmatic. Accordingly, we used an in vivo assay of VP (Miles assay) to study the putative mediators of VEGF/VPF-induced permeability. METHODS AND RESULTS VEGF/VPF and positive controls (platelet-activating factor [PAF], histamine, and bradykinin) all increased vascular permeability. Prior administration of the tyrosine kinase inhibitors genistein or herbimycin A prevented VEGF/VPF-induced permeability. Placenta growth factor, which binds to Flt-1/VEGF-R1 but not Flk-1/KDR/VEGF-R2 receptor tyrosine kinase, failed to increase permeability. Other growth factors such as basic fibroblast growth factor (FGF), acidic FGF, platelet-derived growth factor-BB, transforming growth factor-beta, scatter factor, and granulocyte macrophage-colony stimulating factor (8 to 128 ng) failed to increase permeability. VEGF/VPF-induced permeability was significantly attenuated by the nitric oxide (NO) synthase inhibitors N(omega)-nitro-L-arginine (10 mg/kg) or N(omega)-nitro-L-arginine methyl ester (20 mg/kg) and the cyclooxygenase inhibitor indomethacin (5 mg/kg). The inactive enantiomer N(omega)-nitro-D-arginine methyl ester (20 mg/kg) did not inhibit VEGF/VPF-induced permeability. In vitro studies confirmed that VEGF/VPF stimulates synthesis of NO and prostaglandin metabolites in microvascular endothelial cells. Finally, NO donors and the prostacyclin analogue taprostene administered together but not alone reproduced the increase in permeability observed with VEGF/VPF. CONCLUSIONS These results implicate NO and prostacyclin produced by the interaction of VEGF/VPF with its Flk-1/KDR/VEGF-R2 receptor as mediators of VEGF/VPF-induced vascular permeability. Moreover, this property appears unique to VEGF/VPF among angiogenic cytokines.
JASBO: Jaya Average Subtraction Based Optimization with Deep Learning Model for Multi-Classification of Infectious Disease from Unstructured Data

Infectious diseases have become an unavoidable big trouble in today's environment with a similar symptomatology that makes difficult of early detection and clear separation of infection. Hence, it is required to generate a new technique that best utilizes the various symptomatologies present in the illnesses for its multi-classification. Medical documents are considered an essential source for modern, invented, and robust analysis methods for accurate infection diagnoses. Accordingly, enriching medical text processing is beneficial in health informatics. In this research, proposed Jaya Average Subtraction Based Optimization (JASBO), which is enabled by Deep Learning (DL) is used to classify infectious diseases into many categories from unstructured data. Moreover, the DL model used is Infectious Disease Network (ID-Net) which combines Convolutional Neural Network (CNN) and Bidirectional-Long Short-Term Memory (Bi-LSTM). To specify the strange or discriminative words with BI-LSTM . JASBO algorithm used in the model to determine the size of the filter in the final classification network to detect the meaningful part of the text. The input text is given to the Tokenization layer in this case, where the tokens get formed and is forwarded to CNN. Additionally, character-based network features are extracted using Bi-LSTM model. Then, vector representation is concatenated with two separate character-level extractions from Bi-LSTM and CNN. Character level features are passed to the attention layer, which uses the Kumar-Hassebrook similarity measure to calculate the score function. Label of each word token is then predicted by the ID layer, at which layer size is found by JASBO. Here, JASBO combines Jaya algorithm with an Average and Subtraction-Based Optimizer (ASBO). The best performance of JASBO_ID-Net is analyzed with three performance metrics: accuracy with superior value of 91%, recall with high value of 88.7%, and F-measure with a superior value of 90%.
A randomised dose finding study of oral tacrolimus (FK506) therapy in refractory ulcerative colitis

Background and aims: Immunosuppressive therapy with intravenous ciclosporin is an alternative treatment option to total colectomy for patients with ulcerative colitis (UC), while the benefits of oral administration of tacrolimus are not well defined and are based on reports of several uncontrolled studies. Methods: Patients with refractory active UC were randomly assigned to a high trough concentration (10–15 ng/ml) group (HT group) (n = 21), low trough concentration (5–10 ng/ml) group (LT group) (n = 22), or placebo group (n = 20). Patients received an initial oral dose of 0.05 mg/kg tacrolimus or placebo twice daily. Efficacy was evaluated in 60 patients based on a disease activity index (DAI) score. Fifty eight patients had additional treatment with tacrolimus and were evaluated for efficacy in a 10 week open label extension. Results: An improvement in DAI score (⩾4 points, all categories improved) was observed for 68.4% of cases in the HT group compared with 10.0% in the placebo group (p<0.001). In the HT group, 20.0% of patients had clinical remission and 78.9% had mucosal healing. In the open label extension, 55.2% of all patients had an improved DAI score at week 10. Mean dose of prednisolone was reduced from 19.7 mg/day at study entry to 7.8 mg/day at week 10. The incidence of side effects in the HT group was significantly higher than that of the placebo group (p = 0.043). The most common event was mild finger tremor. Conclusions: Our findings demonstrate dose dependent efficacy and safety of oral tacrolimus for remission-induction therapy of refractory UC. The optimal target range appears to be 10–15 ng/ml in terms of efficacy with two week therapy.