Miracle-dz/UniFAR-FTUs huggingface dataset:
Peroxin Pex21p interacts with the C‐terminal noncatalytic domain of yeast seryl‐tRNA synthetase and forms a specific ternary complex with tRNASer

The seryl‐tRNA synthetase from Saccharomyces cerevisiae interacts with the peroxisome biogenesis‐related factor Pex21p. Several deletion mutants of seryl‐tRNA synthetase were constructed and inspected for their ability to interact with Pex21p in a yeast two‐hybrid assay, allowing mapping of the synthetase domain required for complex assembly. Deletion of the 13 C‐terminal amino acids abolished Pex21p binding to seryl‐tRNA synthetase. The catalytic parameters of purified truncated seryl‐tRNA synthetase, determined in the serylation reaction, were found to be almost identical to those of the native enzyme. In vivo loss of interaction with Pex21p was confirmed in vitro by coaffinity purification. These data indicate that the C‐terminally appended domain of yeast seryl‐tRNA synthetase does not participate in substrate binding, but instead is required for association with Pex21p. We further determined that Pex21p does not directly bind tRNA, and nor does it possess a tRNA‐binding motif, but it instead participates in the formation of a specific ternary complex with seryl‐tRNA synthetase and tRNASer, strengthening the interaction of seryl‐tRNA synthetase with its cognate tRNASer.
Vortex-induced vibrations of a flexible cylinder at large inclination angle

The free vibrations of a flexible circular cylinder inclined at 80° within a uniform current are investigated by means of direct numerical simulation, at Reynolds number 500 based on the body diameter and inflow velocity. In spite of the large inclination angle, the cylinder exhibits regular in-line and cross-flow vibrations excited by the flow through the lock-in mechanism, i.e. synchronization of body motion and vortex formation. A profound reconfiguration of the wake is observed compared with the stationary body case. The vortex-induced vibrations are found to occur under parallel, but also oblique vortex shedding where the spanwise wavenumbers of the wake and structural response coincide. The shedding angle and frequency increase with the spanwise wavenumber. The cylinder vibrations and fluid forces present a persistent spanwise asymmetry which relates to the asymmetry of the local current relative to the body axis, owing to its in-line bending. In particular, the asymmetrical trend of flow–body energy transfer results in a monotonic orientation of the structural waves. Clockwise and counter-clockwise figure eight orbits of the body alternate along the span, but the latter are found to be more favourable to structure excitation. Additional simulations at normal incidence highlight a dramatic deviation from the independence principle, which states that the system behaviour is essentially driven by the normal component of the inflow velocity.
OPC UA integration for field devices

In industrial automation systems, deploying the well-established automation pyramid model is best practice. However, the trend to massively distributed systems, which are foreseen to co-operate using standardized protocols and common semantics, shows the limits of these traditional approaches. In order to enable for Industry 4.0 compliant solutions, appropriate means for scalable internetworking have to be developed and utilized. New modelling technologies have been developed to represent such distributed automation systems, incorporating a multidimensional layered approach. At higher hierarchy levels of these automation models, standardization approaches are quite common. However, at the field layer there are still many different field busses, which in most cases do not allow common semantics, but come along with their own object models. In contrast, the use of OPC UA at the field level, with its standardized protocol stack and semantic annotations, would allow for enabling field devives to fully participate in large scaled systems as Industry 4.0 components. This paper evaluates the potentials and limits of integrating OPC UA into legacy field devices with limited communication and calculation resources and provides quantitative measurement results of selected test scenarios.
The Taxation of Personal Wealth in International Perspective

The revenue importance of taxes on net wealth and capital transfers is diminishing almost everywhere. In general, however, those developed countries with such taxes show no sign of abandoning them. The main exceptions are Australia and Canada, which have never had net wealth taxes and which have, like a number of developing countries, abolished death taxes. Although Canada has further emulated some developing countries by introducing a new wealth tax on corporations, only a major political change (like the election of socialist governments in France and Spain) seems likely to result in the reintroduction of direct taxes on personal wealth in this country.
Design methodology of LLC converters based on mode analysis for battery charging applications

In this paper, an LLC resonant converter design methodology for battery charger based on mode analysis is presented. The accuracy of DC gain curve obtained by mode analysis is much higher than that by FHA. Conduction loss and switching loss can be minimized by reasonably configuring charging trajectory on the mode distribution map. ZVS and ZCS can be realized during whole charging process. Finally, a 700W charger prototype with a peak efficiency of 96.1% was built to verify the effectiveness of design method.
Bike-sharing: History, Impacts, Models of Provision, and Future

This paper discusses the history, impacts, models of provision, and future of bike-sharing, or public bicycle programs. The author covers the history of bike-sharing from the early 1st generation program to present day 3rd generation programs, presenting a detailed examination of models of provision, with benefits and detriments of each, and a description of capital and operating costs. In the government model, the locality operates the bike-sharing program must as it would any other transit service. The university model has the educational institution providing the service, most likely in a campus setting. The non-profit model has an organization that was either expressly created for the operation of the bike-sharing service or one that folds the bike-sharing service into its existing interests. With the advertising company model, companies offer a bike-sharing program to a jurisdiction, usually in exchange for the right to use public space to display revenue-generating advertisements. And in the for-profit model, a private company provides the service with limited or no government involvements. The author maintains that there is no one ideal model that is appropriate for all situations. The third-generation of bike-sharing brought the use of smartcards, mobile phones, and kiosks with screens; the fourth generation will be distinguished by improved efficiency, sustainability, and usability. This will be achieved by improving distribution of bikes, installation, powering of stations, tracking, offering pedal assistance bikes, and new business models. The author discusses each of these improvements and concludes that the era of bike-sharing has just begun.
Retos para la educación y profesión contable derivados de una armonización mundial liderada por el IASC: la nueva estrategia europea de armonización contable como modelo para otras áreas económicas

The objective of this paper is to analyse the important decisions made by the European Commission in 2000, in order to undertake a final strategy with regard to accounting harmonisation. We think that this topic is especially relevant in the framework of this International Conference, because many regional accounting harmonising bodies may follow the EU steps since Europe is a very important economic area to be borne in mind in the world accounting harmonisation debate where the United States are playing the leading role on the grounds of their capital markets hegemony. Given the timeliness and relevance of these regulatory changes, we think it is of the outmost importance to look into their origins and highlight their impact on the everyday life of the different components of the accounting discipline, i.e., regulators, academics and the accounting profession.The "widely unknown" IAS are just "round the corner". It is about time that we all start preparing the change of the basis of financial reporting to IAS and get ready for the challenges that it poses to the accounting profession and education.
Image-Guided Surgery Influences Perioperative Morbidity from Endoscopic Sinus Surgery

Objective Although image-guided surgery (IGS) is considered a valuable tool, its impact on perioperative morbidity for endoscopic sinus surgery (ESS) remains unclear. The evidence from reported literature is systematically reviewed with meta-analysis. Data Sources MEDLINE (1946 to September 14, 2012, week 2) and EMBASE (1974 to September 14, 2012, week 37). Review Methods MEDLINE and EMBASE were searched using a search strategy for publications on IGS during ESS that reported original data on perioperative morbidity. PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) guidelines were followed. Both comparative cohort studies with non-IGS cases and case series were included. Primary outcome was major and total complications. Secondary outcomes were specific orbital and intracranial injury, major hemorrhage, ability to complete the operation, and revision surgery. The incidence of these events was defined as dichotomous variables and expressed as a risk ratio (RR) in a fixed-effects model. Results In total, 2586 articles fulfilled the search, producing 55 included studies. Fourteen were comparative cohorts of IGS and non-IGS sinus surgical patient populations used for meta-analysis. Among the cohorts, major complications were more common in the non-IGS group (RR = 0.48; 95% confidence interval [CI], 0.28-0.82; P = .007). Total complications were greater in the non-IGS group (RR = 0.66; 95% CI, 0.47-0.94; P = .02). All other outcomes did not reach significance on meta-analysis. Conclusion Contrary to current review articles on the topic of IGS use during ESS, there is evidence from published studies that the use of IGS for sinus surgery, within selected populations, is associated with a lower risk of major and total complications compared with non-IGS sinus surgery.
American Gastroenterological Association (AGA) Institute medical position statement on obscure gastrointestinal bleeding.

This document presents the official recommendations of the American Gastroenterological Association (AGA) Institute on "Evaluation and Management of Occult and Obscure Gastrointestinal Bleeding. " It was approved by the Clinical Practice and Economics Committee on March 12, 2007, and by the AGA Institute Governing Board on May 19, 2007. This medical position statement is based upon the interpretation and assimilation of scientifically valid research, derived from a comprehensive review of published literature.
Fiscal Consolidation: Part 1. How Much is Needed and How to Reduce Debt to a Prudent Level?

The economic and financial crisis was the catalyst for a fiscal crisis that engulfs many OECD countries. In most countries, budget deficits soared as a result of the economic slump, weaker revenues and the policy response to the crisis. Consolidating the public finances is an important challenge for many countries. Estimates of fiscal gaps suggest that substantial and sustained fiscal tightening will be needed in nearly all countries to bring debt down to prudent levels. However, given a weak global economy, implementing a large fiscal tightening could be particularly costly. Structuring consolidation packages to use instruments with low multipliers initially and enhancing the institutional framework for fiscal policy to lend greater credibility to the commitment to consolidate over time may help minimise the trade-offs with growth in the short run. In most countries there is scope to target spending programmes more effectively and eliminate distortions in taxation and re-orientate taxation to minimise distortions. Such measures, buttressed by structural reforms, such as to unsustainable pension systems, can underpin fiscal sustainability, while minimising the costs to long-run growth. Consolidation budgetaire: Partie 1. Quel est le degre d'assainissement necessaire et comment ramener la dette a un niveau prudent ? La crise economique et financiere a servi de catalyseur pour une crise budgetaire qui engloutit de nombreux pays de l’OCDE. Dans la plupart des pays, les deficits budgetaires ont fait un bond en raison du marasme economique, de la diminution des recettes et des mesures prises en reponse a la crise. Assainir les finances publiques represente un enjeu majeur pour bon nombre de pays. Sur la base d’estimations des ecarts budgetaires, il semble qu’il faudra un resserrement budgetaire important et durable dans presque tous les pays pour ramener la dette a des niveaux prudents. Cependant, compte tenu de la faiblesse de l’economie mondiale, la mise en oeuvre d’un vaste programme de restriction budgetaire pourrait etre particulierement couteuse. Structurer les programmes de consolidation de facon a utiliser au depart des instruments a multiplicateurs faibles et, a terme, a ameliorer le cadre institutionnel de la politique budgetaire afin de rendre plus credible l’engagement a assainir les finances publiques pourrait aider a reduire au minimum les arbitrages avec la croissance dans le court terme. Dans la plupart des pays, il est possible de cibler plus efficacement les programmes de depenses et d’eliminer les distorsions dans la fiscalite et de reorienter cette derniere afin de reduire au minimum les distorsions. Ces mesures, etayees par des reformes structurelles telles que la reforme des systemes de retraite, qui ne sont plus tenables, peuvent soutenir la viabilite budgetaire tout en reduisant au minium les couts pour la croissance a long terme.
Inequality, Development, and the Stability of Democracy -Lipset and Three Critical Junctures in German History

This paper studies the endogenous emergence of political regimes in societies in which productive resources are distributed unequally and institutions do not ensure political commitments. The results imply that for any level of development there exists a distribution of resources such that democracy emerges in equilibrium, but there are distributions of resources for which democracy is infeasible in equilibrium irrespective of the level of development. The model also delivers results on the stability of democracy with regard to changes in the economic and demographic environment. The results are consistent with the different political regimes that emerged in Germany after 1871.
Prognosis after ipsilateral breast tumor recurrence and locoregional recurrences in five National Surgical Adjuvant Breast and Bowel Project node-positive adjuvant breast cancer trials.

PURPOSE Locoregional failure after breast-conserving surgery is associated with increased risk of distant disease and death. The magnitude of this risk in patients receiving chemotherapy has not been adequately characterized. PATIENTS AND METHODS Our study population included 2,669 women randomly assigned onto five National Surgical Adjuvant Breast and Bowel Project node-positive protocols (B-15, B-16, B-18, B-22, and B-25), who were treated with lumpectomy, whole-breast irradiation, and adjuvant systemic therapy. Cumulative incidences of ipsilateral breast tumor recurrence (IBTR) and other locoregional recurrence (oLRR) were calculated. Kaplan-Meier curves were used to estimate distant-disease-free survival (DDFS) and overall survival (OS) after IBTR or oLRR. Cox models were used to model survival using clinical and pathologic factors jointly with IBTR or oLRR as time-varying predictors. RESULTS Four hundred twenty-four patients (15.9%) experienced locoregional failure; 259 (9.7%) experienced IBTR, and 165 (6.2%) experienced oLRR. The 10-year cumulative incidence of IBTR and oLRR was 8.7% and 6.0%, respectively. Most locoregional failures occurred within 5 years (62.2% for IBTR and 80.6% for oLRR). Age, tumor size, and estrogen receptor status were significantly associated with IBTR. Nodal status and estrogen and progesterone receptor status were significantly associated with oLRR. The 5-year DDFS rates after IBTR and oLRR were 51.4% and 18.8%, respectively. The 5-year OS rates after IBTR and oLRR were 59.9% and 24.1%, respectively. Hazard ratios for mortality associated with IBTR and oLRR were 2.58 (95% CI, 2.11 to 3.15) and 5.85 (95% CI, 4.80 to 7.13), respectively. CONCLUSION Node-positive breast cancer patients who developed IBTR or oLRR had significantly poorer prognoses than patients who did not experience these events.
The role of alcohol policies in preventing intimate partner violence: a review of the literature.

OBJECTIVE This article summarizes existing research on the relationship between alcohol policies and intimate partner violence (IPV). Because alcohol use represents an important risk factor for IPV, interventions and policies aimed at decreasing problem drinking may also lead to reductions in IPV. METHOD Electronic databases were searched to identify relevant peer-reviewed journal articles on alcohol policies and IPV, as well as reference sections of appropriate articles. Only policies that have been studied specifically for their impact on IPV were included. RESULTS Three alcohol policy areas (outlet density, hours and days of sale, and pricing/taxation) have been studied in relation to IPV outcomes. Research on outlet density has the most consistent findings, with most studies indicating that higher densities of alcohol outlets are associated with higher rates of IPV. Fewer studies have been conducted on pricing policies and policies restricting hours/days of sale, with most studies suggesting no impact on IPV rates. CONCLUSIONS A higher density of alcohol outlets appears to be associated with greater rates of IPV. However, there is limited evidence suggesting that alcohol pricing policies and restrictions on hours and days of sale are associated with IPV outcomes. Knowledge about the impact of alcohol-related policies on IPV and violence in general is limited by several significant research gaps. Additional research is needed to assess the impact of alcohol policies on IPV and other forms of violence.
Negative pressure suction during blood sampling may reduce the difference in self-monitoring of blood glucose results between fingertip pricking and forearm pricking.

Self-monitoring of blood glucose (SMBG) at alternate puncture sites, such as the forearm, gives users an alternative to fingertip puncture, which can be painful. However, it has been reported that blood glucose values obtained at alternate sites are different from those obtained at the fingertip. We aimed to examine whether SMBG results obtained at the forearm are clinically identical to those at the fingertip and whether blood sampling technique by negative pressure suction could reduce the fingertip-to-forearm differences. Five male volunteers were recruited. A 75-g oral glucose tolerance test (OGTT) identified four individuals with impaired glucose tolerance and one with diabetes. Subject age, HbA1c, fasting venous plasma glucose, and BMI were 50.4 ± 2.1 years, 5.2 ± 0.3%, 95 ± 10 mg/dl, and 23.1 ± 0.6 kg/m2, respectively (means ± SE). Each participant was fully informed about the experiment and signed the consent form before the study began. OGTT and insulin tolerance test (ITT) were continuously performed in the fasting state. OGTT was performed using the usual methods; blood glucose levels were monitored at 0, 30, 60, 90, and 120 min after the ingestion of a 75-g glucose solution. After the blood collection at 120 min of the OGTT, ITT was started by intravenous administration of insulin at 0.1 unit/1 kg body wt. The …
Familial Correlations, Segregation Analysis, and Nongenetic Correlates of Soy Isoflavone–Metabolizing Phenotypes

Particular intestinal bacteria metabolize the soy isoflavone daidzein to equol and O-desmethylangolensin (O-DMA), metabolites that can be identified in urine. Individuals that harbor bacteria capable of producing equol or O-DMA are known as equol producers (approximately 30%-50% of the population) and O-DMA producers (approximately 80%-90% of the population), respectively. The equol-producer phenotype has been associated with sex hormone-related outcomes in several studies. However, the bacteria responsible for these phenotypes have not yet been identified and factors that influence the manifestation of these phenotypes are not well understood. To evaluate familial clustering of and nongenetic factors associated with these phenotypes, 410 individuals from 112 families participated in phenotyping (3-day soy challenge and Day 4 spot urine collection). In segregation analyses of the equol-producer phenotype, the Mendelian dominant model provided the most parsimonious fit to the data, suggesting that the pattern of inheritance of the equol-producer phenotype is consistent with an autosomal dominant trait. This phenotype was positively associated with education (p trend = 0.01), but not with sex, smoking, or several dietary factors. Results of the segregation analyses of the O-DMA-producer phenotype were inconclusive; no other models provided a more parsimonious fit to the data than the general model. This phenotype was inversely associated with age in a nonlinear model (p = 0.01), positively associated with age- and sex-adjusted height (odds ratio [OR] 10-cm increase = 0.38, 95% confidence interval [CI] = 0.15, 0.95) and body mass index (kg/m2) (OR = 0.91, 95% CI = 0.85, 0.96), but not with sex, education, smoking, or several dietary factors. These results suggest the equol-producer phenotype may be under some degree of genetic control and that there are likely other environmental factors not evaluated in the present analysis that contribute to both of these phenotypes. These results provide a foundation for further work to refine our understanding of heritable and environmental determinants of daidzein-metabolizing phenotypes.
Molecular Mechanism of the Bifunctional Role of Lipopolysaccharide in Osteoclastogenesis*

Lipopolysaccharide (LPS), a common bacteria-derived product, has long been recognized as a key factor implicated in periodontal bone loss. However, the precise cellular and molecular mechanisms by which LPS induces bone loss still remains controversial. Here, we show that LPS inhibited osteoclastogenesis from freshly isolated osteoclast precursors but stimulated osteoclast formation from those pretreated with RANKL in vitro in tissue culture dishes, bone slices, and a co-culture system containing osteoblasts, indicating that RANKL-mediated lineage commitment is a prerequisite for LPS-induced osteoclastogenesis. Moreover, the RANKL-mediated lineage commitment is long term, irreversible, and TLR4-dependent. LPS exerts the dual function primarily by modulating the expression of NFATc1, a master regulator of osteoclastogenesis, in that it abolished RANKL-induced NFATc1 expression in freshly isolated osteoclast precursors but stimulated its expression in RANKL-pretreated cells. In addition, LPS prolonged osteoclast survival by activating the Akt, NF-κB, and ERK pathways. Our current work has not only unambiguously defined the role of LPS in osteoclastogenesis but also has elucidated the molecular mechanism underlying its complex functions in osteoclast formation and survival, thus laying a foundation for future delineation of the precise mechanism of periodontal bone loss.