Miracle-dz/UniFAR-FTUs huggingface dataset:
Don't Vote for Them: The Effects of the Spanish Indignant Movement on Attitudes about Voting

Abstract This article investigates the recent so-called Spanish Revolution of 2011 with a view to understanding what it reveals about the current relationship between protest and electoral modes of participation. Theories of “disaffeccted radicalism” that grew up following the 1960s period of civil unrest strongly advocated the view that protest activity boosted electoral abstention. More recent work on protest, however, has pointed to its “normalization” and linkage to more conventional modes of participation. The Spanish case of 15M constitutes a useful new test of the two theories given that it mixed an explicit rejection of the choices voters faced with a criticism of political apathy. I examine the validity of each argument using a four-wave online panel survey and fixed-effects model to unravel how engagement in the 15M protest activity affected Spaniards' attitudes towards voting. The results provide fresh support for the normalization argument about a convergence of electoral and non-electoral types of activity. What is more, protest here seems to have a socialization effect that leads people to look at elections in a more positive manner.
The clinical characteristics and prognostic analysis of Chinese advanced NSCLC patients based on circulating tumor DNA sequencing

Purpose Circulating tumor DNA (ctDNA) is a noninvasive and real-time marker for tumor diagnosis, prognosis, and prediction. However, further investigations about ctDNA prognostic and predictive value are still needed, and conclusions from several studies were inconsistent. Experimental design We performed capture-based targeted ultradeep sequencing on liquid biopsies from a cohort of 34 advanced Chinese non-small-cell lung cancer (NSCLC) patients and analyzed the clinical use of ctDNA in this study. Results On the basis of clinical characteristics of the 34 NSCLC patients, we found that brain metastasis correlated with shorter progression-free survival (PFS) and is more prone to happen in younger patients. After ctDNA sequencing, we analyzed the prognostic value of baseline ctDNA. In osimertinib-treated group, high max allelic fraction (maxAF) correlated with shorter PFS. But for the cohort of 34 patients, no correlation can be observed between maxAF and PFS. We also presented two cases to demonstrate the value of disease progression prediction by ctDNA, which can be detected earlier than clinical response. Conclusion In this study, we demonstrated that ctDNA is a prognostic marker for evaluating treatment response and predicting recurrence in advanced NSCLC. Further investigations with larger cohort and uniformed patient background are still needed to validate our findings.
J O U R N a L O F Veterinary Science Detection of Streptococcus Dysgalactiae Subsp. Equisimilis in Equine Nasopharyngeal Swabs by Pcr

Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection in some animal species can also occur, although a few studies have looked into cross-species infectivity. In horses, S. equisimilis is generally considered infrequent or opportunistic, but has recently been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable epidemiological studies and effective investigation of outbreaks involving these bacteria. In this study, PCR protocols previously described in cattle and in humans to detect the species S. dysgalactiae and the subspecies equisimilis were evaluated to detect specific sequences in equine samples. For this purpose, 99 monolateral nasal swabs were collected from horses from stud farms with a history of S. equisimilis infection and were tested blindly by bacteriological isolation and by single and duplex PCR. DNA for PCR was extracted both from the colonies grown on agar media and from enrichment broth aliquots after incubation with nasal swab samples. S. equisimilis was identified by bacteriological isolation in 23 out of 99 swab samples, and PCR assays on these colonies were fully concordant with bacteriological identification (kappa statistic = 1.00). In addition, PCR of the enrichment broth aliquots confirmed the bacteriological results and detected S. equisimilis in 6 samples more than the bacteriological examination (kappa statistic = 0.84). The PCR protocols appeared to be reliable for the rapid identification of S. equisimilis in equine nasal swab samples, and could be useful for microbiological diagnosis.
Epigenetic Hierarchy within the MAGEA1 Cancer-Germline Gene: Promoter DNA Methylation Dictates Local Histone Modifications

Gene MAGEA1 belongs to a group of human germline-specific genes that rely on DNA methylation for repression in somatic tissues. Many of these genes, termed cancer-germline (CG) genes, become demethylated and activated in a wide variety of tumors, where they encode tumor-specific antigens. The process leading to DNA demethylation of CG genes in tumors remains unclear. Previous data suggested that histone acetylation might be involved. Here, we investigated the relative contribution of DNA methylation and histone acetylation in the epigenetic regulation of gene MAGEA1. We show that MAGEA1 DNA hypomethylation in expressing melanoma cells is indeed correlated with local increases in histone H3 acetylation (H3ac). However, when MAGEA1-negative cells were exposed to a histone deacetylase inhibitor (TSA), we observed only short-term activation of the gene and detected no demethylation of its promoter. As a more sensitive assay, we used a cell clone harboring a methylated MAGEA1/hph construct, which confers resistance to hygromycin upon stable re-activation. TSA induced only transient de-repression of the transgene, and did not lead to the emergence of hygromycin-resistant cells. In striking contrast, transient depletion of DNA-methyltransferase-1 in the reporter cell clone gave rise to a hygromycin-resistant population, in which the re-activated MAGEA1/hph transgene displayed not only marked DNA hypomethylation, but also significant reversal of histone marks, including gains in H3ac and H3K4me2, and losses of H3K9me2. Collectively, our results indicate that DNA methylation has a dominant role in the epigenetic hierarchy governing MAGEA1 expression.
Biallelic expression of the IL‐2 locus under optimal stimulation conditions

Recent experiments have suggested that the IL‐2 locus is monoallelically expressed. We tested this hypothesis using TCR‐transgenic mice carrying one inactivated IL‐2 allele. The frequency in single‐cell assays of IL‐2‐producing cells following optimal stimulation by antigen and antigen‐presenting cells was equivalent to that from wild‐type mice, but the amount of IL‐2 produced per cell was twofold less. Similar observations were made by intracellular staining for IL‐2, although stimulation in bulk culture was less optimal, showing only a 1.7‐fold difference. Importantly, the frequency of responding cells from the heterozygotes was less than from the wild‐type mice if the IL‐2 assay was performed after only 24 – 30 h of activation, suggesting that the targeted allele could compete with the normal allele early after stimulation and give the misimpression that the heterozygotes had fewer IL‐2‐producing cells. These data strongly argue that the IL‐2 locus can be expressed biallelically under optimum stimulation conditions.
Trial by polygraph: Scientific and juridical issues in lie detection

The present article examines two methods of polygraph-assisted lie detection: the Control Question Technique (CQT) and the Guilty Knowledge Technique (GKT). It presents the rationale for both, arguing that only the latter is well grounded in psychological theory. It then surveys the empirical support for claims of the polygraph's ability to detect deception, arguing that such support often comes from studies that are methodologically flawed by contamination of various sorts—especially studies of the CQT. The article then explores the legal implications of introducing polygraph test results, as presently gathered, into the criminal courtroom.
Characterization of the Early Response of Arabidopsis to Alternaria brassicicola Infection Using Expression Profiling[w]

All tested accessions of Arabidopsis are resistant to the fungal pathogen Alternaria brassicicola. Resistance is compromised by pad3 or coi1 mutations, suggesting that it requires the Arabidopsis phytoalexin camalexin and jasmonic acid (JA)-dependent signaling, respectively. This contrasts with most well-studied Arabidopsis pathogens, which are controlled by salicylic acid-dependent responses and do not benefit from absence of camalexin or JA. Here, mutants with defects in camalexin synthesis (pad1, pad2, pad3, and pad5) or in JA signaling (pad1, coi1) were found to be more susceptible than wild type. Mutants with defects in salicylic acid (pad4 and sid2) or ethylene (ein2) signaling remained resistant. Plant responses to A. brassicicola were characterized using expression profiling. Plants showed dramatic gene expression changes within 12 h, persisting at 24 and 36 h. Wild-type and pad3 plants responded similarly, suggesting that pad3 does not have a major effect on signaling. The response of coi1 plants was quite different. Of the 645 genes induced by A. brassicicola in wild-type and pad3 plants, 265 required COI1 for full expression. It is likely that some of the COI1-dependent genes are important for resistance to A. brassicicola. Responses to A. brassicicola were compared with responses to Pseudomonas syringae infection. Despite the fact that these pathogens are limited by different defense responses, approximately 50% of the induced genes were induced in response to both pathogens. Among these, requirements for COI1 were consistent after infection by either pathogen, suggesting that the regulatory effect of COI1 is similar regardless of the initial stimulus.
Filling an Emotional Developmental Void With the Aid of Attuned Selfobject Responsiveness: A Discussion of F. Göver Kazancioğlu's “An Adolescent Journey: Filling the Void With Sound”

This discussion offers a way in which a self psychologically informed developmental and therapeutic approach, emphasizing the selfobject needs and tranferences, may expand the understanding of Ayda's case and affect the ways of intervening. It is suggested that, rather than viewing the patient's use of songs as creating a gap between her and her therapist, it can be viewed as an attempt at bridging the gap which, in her experience, came between them. It is also suggested that the songs do not necessarily convey a hidden, symbolic message that needs deciphering and interpretation but, rather, that it may be useful to consider them as accurately and directly expressing and communicating the patient's developmental trajectory and her evolving state of self.
Missense mutations as a cause of metachromatic leukodystrophy

Metachromatic leukodystrophy is a lysosomal storage disorder caused by a deficiency of arylsulfatase A (ASA). Biosynthesis studies of ASA with various structure‐sensitive monoclonal antibodies reveal that some epitopes of the enzyme form within the first minutes of biosynthesis whereas other epitopes form later, between 10 and 25 min. When we investigated 12 various ASAs, with amino acid substitutions according to the missense mutations found in metachromatic leukodystrophy patients, immunoprecipitation with monoclonal antibodies revealed folding deficits in all 12 mutant ASA enzymes. Eleven of the 12 mutants show partial expression of the early epitopes, but only six of these show, in addition, incomplete expression of late epitopes. In none of the mutant enzymes were the late forming epitopes found in the absence of early epitopes. Thus, data from the wild‐type and mutant enzymes indicate that the enzyme folds in a sequential manner and that the folding of early forming epitopes is a prerequisite for maturation of the late epitopes. All mutant enzymes in which the amino acid substitution prevents the expression of the late forming epitopes are retained in the endoplasmic reticulum (ER). In contrast, all mutants in which a single late epitope is at least partially expressed can leave the ER. Thus, irrespective of the missense mutation, the expression of epitopes forming late in biosynthesis correlates with the ability of the enzyme to leave the ER. The degradation of ER‐retained enzymes can be reduced by inhibitors of the proteasome and ER α1,2‐mannosidase I, indicating that all enzymes are degraded via the proteasome. Inhibition of degradation did not lead to an enhanced delivery from the ER for any of the mutant enzymes.
Ocular mucin gene expression levels as biomarkers for the diagnosis of dry eye syndrome.

PURPOSE To evaluate mRNA levels of the ocular mucins MUC1, MUC2, MUC4, MUC5AC, and MUC7 in conjunctival impression cytology samples from patients with moderate to severe dry eye syndrome (DES) compared with a population of healthy subjects; and to investigate the use of the levels of these mucin genes as biomarkers of DES and subsequently as a potential diagnostic test for DES. METHODS This prospective study commenced in the year 2000 and ended in the year 2009. Thirty-eight patients with DES and 43 age- and sex-matched healthy subjects completed the initial part of the study. Investigations were repeated at a later stage in 16 healthy subjects and 30 patients with DES, which were used as external validation data. Conjunctival impression cytology was performed in all subjects to test gene expression of ocular mucin genes MUC1, MUC2, MUC4, MUC5AC, and MUC7. Statistical analysis was performed to determine whether there was a difference in the levels of mucin gene expression between the two groups of subjects. Sensitivity and specificity of mucin gene expression for the diagnosis of DES was calculated. RESULTS Expressions of MUC1, MUC2, MUC4, and MUC5AC (P < 0.0001) were significantly lower in conjunctival epithelium of patients with DES compared with that in normal subjects. These results were replicated in the external control subject and patient groups. MUC1 expression levels demonstrated the greatest sensitivity (83.3%) and specificity (87.5%) among all genes tested. CONCLUSIONS The data strongly suggest that the expression levels of MUC1 may be used as a diagnostic test in DES for investigational and selective clinical trials.
Acoustic analysis of /l/ in glossectomees

The production of /l/ is examined for pre- and post-operative patients who have undergone surgery in three distinct areas (anterior, posterior or lateral tongue) followed by radiotherapy and reconstruction. Results show F1 and F2 to be raised after surgery in all cases. Normalised measures of tongue height (F1-F0) and extension (F2-F1) revealed no significant change after surgery to the side of the tongue but in the other two categories, results indicated a change normally associated with both raising and fronting of the tongue. The paper compares these results with findings from other studies and considers possible mechanisms for the observed changes. 1. INTRODUCTION Previous work [1] by the authors has focused on the production of /s/ and /sh/ in pre- and postoperative glossectomee speech. Although, these phonemes can be objectively shown to be affected by surgery, change in dentition also plays a critical role and in a large number of cases speech therapy must wait until new dentu.
ZMP-Based Biped Running Control

In this article, a running humanoid robot, HRP-2LR, was presented along with its running pattern generation, its controller, and the experimental result. Applying the proposed controller, HRP-2LR could successfully run with average speed of 0.16 m/s, repeating flight phases of 0.06 s and support phases of 0.3 s. Future work consists of the realization of faster running and online running pattern generation.
Assembly of Latex Particles by Using Emulsion Droplets as Templates. 1. Microstructured Hollow Spheres

We describe an emulsion-based technique that allows the assembly of colloid particles into microstructured or multicomponent clusters (“supraparticles”). The particles are gathered, assembled, and fixed together in the restricted, colloid-size 2D or 3D space provided by emulsion droplets. The process is carried out by multiple modification of the colloid interactions within the particle/droplet system“interaction-tailored colloid assembly”. In the first paper of the series we provide a general description of the method. Then we present the data on the assembly of negatively charged (sulfate) or positively charged (amidine) latexes into ordered hollow spherical supraparticles. The following steps are included in the assembly schemes: (1) modification of latex surface charge and properties, so the microspheres are able to adsorb on the droplet surfaces but without homocoagulation; (2) adsorption and structure formation around the emulsion drops (at this stage the interfacial mobility of the particles is of.
Time Tagging: A Key to Musicians' Superior Memory

Years of formal music training and proficiency at a nonmusical skill—— verbal recall——are surprisingly associated (e.g., A. R. Kilgour, L. S. Jakobson, & L. L. Cuddy, 2000). The present study proposes an indirect mechanism to account for this association. It is proposed that music training strengthens auditory temporal-order processing, and that temporalorder processing then mediates the relationship between years of music training and prose recall.
Rosuvastatin safety: lessons from the FDA review and post-approval surveillance

Rosuvastatin is the first statin approved by the regulatory authorities since the withdrawal of cerivastatin. Although highly efficacious, this new statin has generated considerable controversy regarding its safety. Rosuvastatin was approved for clinical use based on the largest pre-approval database for all statins prior to commercial use. In this database, rosuvastatin had a similar safety profile to other approved statins up to the highest approval dose of 40 mg. As with all statins, there is a marked increase in adverse effects when the dose is titrated from 40 to 80 mg, and rosuvastatin demonstrates a similar dose/toxicity relationship. In the pre-approval data trials on 80 mg, there was a 1.0% (n = 16) incidence of myopathy and 7 patients developed rhabdomyolysis. However the ≤ 40 mg doses had a myopathy rate similar to other statins. In the post-marketing surveillance for rosuvastatin, there have been reports of rhabdomyolysis, but the incidence rate, when corrected for prescription utilisation, is similar to other statins following initial approval.
Improving Testability and Reuse by Transitioning to Functional Programming

Declarative styles such as functional programming (FP) are rapidly gaining ground on their imperative cousins, including procedural and object-oriented programming. The shift is subtle because it is happening within the context of multiparadigm programming languages such as JavaScript. FP is better suited to modern processes like test-driven development (TDD), and architectures like massively parallel, cloud-based computing. This article describes the technical details that characterize the shift from imperative to FP and implications for software quality management, particularly reuse and testability.
Improvements of switching energy of SiC transistor with respect of chip integrity and EMC standards

This paper deals with the reduction of the switching energy losses of a SiC transistor by boosting its gate current. Notoriously, wide band-gap semiconductor transistors exhibit very fast switching performances, which provoke undesirables oscillations at high frequencies. An auxiliary circuit, located at the semiconductor device terminals, is developed to limit the electrical stresses, deleterious for the transistor integrity, and to reduce the EMC conducted interferences without modifying the current and voltage slopes during the switching transition. Firstly, the SiC MosFET is characterized on SABER environment from MosFET template available on the library. The behavior of the obtained model is compared with Model Architect tool, available in the same environment. Experimental tests are carried out and confirm the behaviors predicted by simulation. The obtained results underlines the good accuracy and the merits of the simulation model developed in this work, despite the use of the power transistor in a complex configuration.
Multimodal Storytelling via Generative Adversarial Imitation Learning

Deriving event storylines is an effective summarization method to succinctly organize extensive information, which can significantly alleviate the pain of information overload. The critical challenge is the lack of widely recognized definition of storyline metric. Prior studies have developed various approaches based on different assumptions about users' interests. These works can extract interesting patterns, but their assumptions do not guarantee that the derived patterns will match users' preference. On the other hand, their exclusiveness of single modality source misses cross-modality information. This paper proposes a method, multimodal imitation learning via Generative Adversarial Networks(MIL-GAN), to directly model users' interests as reflected by various data. In particular, the proposed model addresses the critical challenge by imitating users' demonstrated storylines. Our proposed model is designed to learn the reward patterns given user-provided storylines and then applies the learned policy to unseen data. The proposed approach is demonstrated to be capable of acquiring the user's implicit intent and outperforming competing methods by a substantial margin with a user study.
Weight lifting can facilitate appreciative comprehension for museum exhibits

Appreciation of exhibits in a museum can be equated to a virtual experience of lives in the contexts originally surrounding the exhibits. Here we focus on the importance of weight information, and hence tested whether experiencing a weight during museum exhibit appreciation affects the beholders' satisfaction and recognition memory for the exhibits. An experiment was performed at a museum exhibiting skeletal preparations of animals. We used nine preparations and prepared four weight stimuli as weight cues in accordance with the actual weight of four of the preparations: Remaining five preparations was displayed without weight stimuli. In the cued condition, participants were asked to lift up the weight stimuli during their observation of the four exhibits. In the uncued condition, participants observed the exhibits without touching the weight stimuli. After observation of the exhibits, the participants responded to a questionnaire that measured their impressions of the exhibits and the museum, and performed a recognition test on the exhibits. Results showed that memory performance was better and viewing duration was longer with weight lifting instruction than without instruction. A factor analysis on the questionnaires revealed four factors (likeability, contentment, value, and quality). A path analysis showed indirect effects of viewing duration on memory performance and willingness-to-pay (WTP) for the museum appreciation through the impression factors. Our findings provide insight into a new interactive exhibition that enables long appreciation producing positive effects on visitors' impression, memory, and value estimation for exhibits.