Miracle-dz/UniFAR-FTUs huggingface dataset:
International Interventions to Build Social Capital: Evidence from a Field Experiment in Sudan

Increasingly the international community attempts to improve local public infrastructure in developing countries by creating more participatory local governance and social capital. We report on a randomized field experiment conducted in 24 communities (16 treated and 8 control) in rural Sudan. We offer a clearer theoretical statement of how these programs might alter the political landscape of the recipient villages. We measure norms using lab-in-the-field techniques and we measure network density with a survey of our 475 lab subjects. We appraise the participatory character of local governance and civic participation with a survey of 576 households. The program did not affect either networks or norms, but civic participation and the participatory nature of local governance increased. Thus we attribute the increase in citizen participation not to social capital growth but to more open local governing institutions.
Title: 1 Atp and the Purine Type 2 X7 Receptor Affect Sleep 2

Title: 1 ATP and the purine type 2 X7 receptor affect sleep 2 Authors and author addresses: 3 James M. Krueger, Ping Taishi, Alok De, Christopher J. Davis, Bradley D. 4 Winters, James Clinton, Éva Szentirmai , Mark R. Zielinski 5 Sleep and Performance Research Center, Programs in Neuroscience, and 6 College of Veterinary Medicine, Washington State University, Pullman, 7 WA, USA 99164-6520 8 2 Current address: Department of Obstetrics and Gynecology, School of 9 Medicine, University of Missouri-Kansas City, Kansas City, MO 64108 10 3 WWAMI Medical Education Program, Washington State University, 11 Spokane, WA 99210-1495 12 Running head: 13 ATP via P2 receptors regulates sleep 14 Corresponding author with complete address, including an email address: 15 James M. Krueger 16 Department of Veterinary and Comparative Anatomy, Pharmacology and 17 Physiology, College of Veterinary Medicine, Washington State University, 18 Pullman, WA, 99164-6520, U.S.A. 19 krueger@vetmed.wsu. edu 20 21 22 23 Articles in PresS. J Appl Physiol (September 9, 2010). doi:10.1152/japplphysiol.00586. 2010
Muscle damage responses to resistance exercise performed with high-load versus low-load associated with partial blood flow restriction in young women

Abstract The aim of this study was to compare if an acute exercise session of high-load resistance training (HL-RT, e.g. 70% of 1 repetition-maximum, 1 RM) induces a higher magnitude of muscle damage compared with a RT protocol with low-loads (e.g. 20% 1 RM) associated with partial blood flow restriction (LL-BFR), and investigate the recovery in the days after the protocols. We used an unilateral crossover research design in which 10 young women (22(2) y; 162(5) cm; 66(11) kg) performed HL-RT and LL-BFR in a randomized, counterbalanced manner with a minimum interval of 2 weeks between protocols. Indirect muscle damage markers were evaluated before and once a day for 4 days into recovery. Main results showed decreases of 8–12% at 24–48 h in maximal voluntary isometric and concentric contraction torques (P < 0.03), and changes in muscle architecture markers (P < 0.03) for HL-RT and LL-BFR, with no differences between protocols (P > 0.05). Moreover, delayed onset muscle soreness increased only after LL-BFR (P < 0.001). We conclude that an acute bout of low volume HL-RT or LL-BFR to failure resulted in edema-induced muscle swelling, but do not induce major or long-lasting decrements in muscle function and the level of soreness promoted from LL-BFR was mild.
S100A12 Suppresses Pro-inflammatory, but Not Pro-Thrombotic Functions of Serum Amyloid A

S100A12 is elevated in the circulation in patients with chronic inflammatory diseases and recent studies indicate pleiotropic functions. Serum amyloid A induces monocyte cytokines and tissue factor. S100A12 did not stimulate IL-6, IL-8, IL-1β or TNF-α production by human peripheral blood mononuclear cells but low amounts consistently reduced cytokine mRNA and protein levels induced by serum amyloid A, by ∼49% and ∼46%, respectively. However, S100A12 did not affect serum amyloid A-induced monocyte tissue factor. In marked contrast, LPS-induced cytokines or tissue factor were not suppressed by S100A12. S100A12 did not alter cytokine mRNA stability or the cytokine secretory pathway. S100A12 and serum amyloid A did not appear to form complexes and although they may have common receptors, suppression was unlikely via receptor competition. Serum amyloid A induces cytokines via activation of NF-κB and the MAPK pathways. S100A12 reduced serum amyloid A-, but not LPS-induced ERK1/2 phosphorylation to baseline. It did not affect JNK or p38 phosphorylation or the NF-κB pathway. Reduction in ERK1/2 phosphorylation by S100A12 was unlikely due to changes in intracellular reactive oxygen species, Ca2+ flux or to recruitment of phosphatases. We suggest that S100A12 may modulate sterile inflammation by blunting pro-inflammatory properties of lipid-poor serum amyloid A deposited in chronic lesions where both proteins are elevated as a consequence of macrophage activation.
Expansion of Stem Cell-Like CD4+ Memory T Cells during Acute HIV-1 Infection Is Linked to Rapid Disease Progression

Understanding the immunopathology of acute HIV-1 infection will help to develop eradication strategies. We demonstrate here that a CD4+ T cell memory subset expands during acute HIV-1 infection, which is associated with disease progression. ABSTRACT Acute HIV-1 infection is characterized by high viremia and massive depletion of CD4+ T cells throughout all tissue compartments. During this time the latent viral reservoir is established but the dynamics of memory CD4+ T cell subset development, their infectability and influence on disease progression during acute HIV-1 infection has not been carefully described. We therefore investigated the dynamics of CD4+ T cell memory populations in the RV217 (ECHO) cohort during the acute phase of infection. Interestingly, while we found only small changes in central or effector memory compartments, we observed a profound expansion of stem cell-like memory CD4+ T cells (SCM) (2.7-fold; P < 0.0001). Furthermore, we demonstrated that the HIV-1 integration and replication preferentially take place in highly differentiated CD4+ T cells such as transitional memory (TM) and effector memory (EM) CD4+ T cells, while naive and less mature memory cells prove to be more resistant. Despite the relatively low frequency of productively infected SCM, we suggest that their quiescent phenotype, increased susceptibility to HIV-1 integration compared to naive cells and extensive expansion make them one of the key players in establishment and persistence of the HIV-1 reservoir. Moreover, the expansion of SCM in acute HIV-1 infection was a result of Fas upregulation on the surface of naive CD4+ T cells. Interestingly, the upregulation of Fas receptor and expansion of SCM in acute HIV-1 infection was associated with the early viral set point and disease progression (rho = 0.47, P = 0.02, and rho = 0.42, P = 0.041, respectively). Taken together, our data demonstrate an expansion of SCM during early acute HIV-1 infection which is associated with disease outcome. IMPORTANCE Understanding the immunopathology of acute HIV-1 infection will help to develop eradication strategies. We demonstrate here that a CD4+ T cell memory subset expands during acute HIV-1 infection, which is associated with disease progression.
Sleep and Stroke.

Stroke is the leading cause of serious long-term disability in the United States, leaving less than half of survivors able to return directly home. Stroke recurrence, estimated as high as 17% over 5 years, also remains unacceptably high. The use of lytic drugs and endovascular devices has revolutionized the care of select stroke patients in the acute setting. However, enormous challenges remain in changing the trajectory of stroke recovery for the vast majority of patients who do not qualify or remain disabled after these treatments, and also in preventing the accumulating disability associated with stroke recurrence. The role of sleep disorders in stroke outcome and recurrence has become a pressing question. Despite estimates of greater than 50% prevalence of sleep disorders after stroke, only about 6% of stroke survivors are offered formal sleep testing and an estimated 2% complete such testing in the 3-month poststroke period. The reasons for the low rate of screening are at least partly related to the lack of awareness about sleep disorders among stroke providers (Figure). This review evaluates the role of sleep disorders, including sleepdisordered breathing (SDB) and sleep-wake cycle disorders, in stroke risk and examines the impact of their treatment on stroke outcome.
MicroRNA-155 Expression Is Enhanced by T-cell Receptor Stimulation Strength and Correlates with Improved Tumor Control in Melanoma

T-cell receptor stimulation strength dictates microRNA-155 expression in CD8+ T cells. High miR-155 levels and downregulation of its targets correlate with tumor control in melanoma, suggesting their utility as a CD8+ T-cell responsiveness biomarker. microRNAs are short noncoding RNAs that regulate protein expression posttranscriptionally. We previously showed that miR-155 promotes effector CD8+ T-cell responses. However, little is known about the regulation of miR-155 expression. Here, we report that antigen affinity and dose determine miR-155 expression in CD8+ T cells. In B16 tumors expressing a low-affinity antigen ligand, tumor-specific infiltrating CD8+ T cells showed variable miR-155 expression, whereby high miR-155 expression was associated with more cytokine-producing cells and tumor control. Moreover, anti–PD-1 treatment led to both increased miR-155 expression and tumor control by specific CD8+ T cells. In addition, miR-155 overexpression enhanced exhausted CD8+ T-cell persistence in the LCMV cl13 chronic viral infection model. In agreement with these observations in mouse models, miR-155 expression in human effector memory CD8+ T cells positively correlated with their frequencies in tumor-infiltrated lymph nodes of melanoma patients. Low miR-155 target gene signature in tumors was associated with prolonged overall survival in melanoma patients. Altogether, these results raise the possibility that high miR-155 expression in CD8+ tumor-infiltrating T cells may be a surrogate marker of the relative potency of in situ antigen-specific CD8+ T-cell responses.
Chapter 2 Calm Technology for Biofeedback : Why and How ?

We discuss several possibilities and fundamental difficulties when designing biofeedback systems based on calm technology. As a carrier for the discussion, we develop a novel biofeedback installation based on heart rate variability (HRV). The system is built-in to an elegant table and gives visual feedforward or feedback for relaxation based on breathing. When in feedforward mode, the system will show a sine wave of about 7 cycles per second, close to the well-known resonant breathing frequency. Alternatively, the amplitude of the movement can give feedback on the heart rate variability level, which is known to be directly associated with a reduced level of mental stress. The demonstrator has a pulse-plethysmography sensor which measures the beat-to-beat intervals of successive heart beats. The mechanical design of the actuator is designed to operate completely noiseless. Both the adaptive algorithm and the actuator are new to the best of our knowledge. Still new fundamental questions arise.
Parallelizing Plan Recognition

Modern multicore computers provide an opportunity to parallelize plan recognition algorithms to decrease runtime. Viewing plan recognition as parsing based on a complete breadth first search, makes ELEXIR (engine for lexicalized intent recognition) (Geib 2009; Geib and Goldman 2011) particularly suited for parallelization. This article documents the extension of ELEXIR to utilize such modern computing platforms. We will discuss multiple possible algorithms for distributing work between parallel threads and the associated performance wins. We will show, that the best of these algorithms provides close to linear speedup (up to a maximum number of processors), and that features of the problem domain have an impact on the achieved speedup.
Transposon mutagenesis, characterization, and cloning of transformation genes of Haemophilus influenzae Rd

A plasmid library of PstI fragments of Haemophilus influenzae Rd genomic DNA was mutagenized in Escherichia coli with mini-Tn10kan. The mutagenized PstI fragments were introduced by transformation into the H. influenzae chromosome, and kanamycin-resistant transformants were screened for the transformation-deficient phenotype by a cyclic AMP-DNA plate method. Fifty-four mutant strains containing 24 unique insertions that mapped to 10 different PstI fragments were isolated. Strains carrying unique insertions were tested individually for DNA uptake, transformation efficiency, UV sensitivity, and growth rate. The transformation frequencies of these mutants were decreased by factors of 10(-2) to 10(-6). Five of the mutants had normal competence-induced DNA uptake, and the rest were variably deficient in competence development. Three strains were moderately UV sensitive. All strains but one had doubling times within 50% of that of the wild type. Mutated genes were cloned into an H. influenzae-E. coli shuttle vector, and wild-type loci were recovered by in vivo recombinational exchange. Hybridization of these clones to SmaI genomic fragments separated in pulsed-field gels showed that these insertions were not clustered in a particular region of the chromosome.
Adaptive Relaying in Noncoherent Wireless Relay Networks

This paper considers a cooperative network in which binary frequency-shift-keying (BFSK) modulation is employed to facilitate noncoherent communications between a source and a destination with the help of K relays. Proposed is an adaptive cooperative scheme that employs two thresholds as follows. One threshold is used to select retransmitting relays: a relay retransmits to the destination if its decision variable is larger than the threshold, otherwise it remains silent. The other threshold is used at the destination for detection: the destination marks a relay as a retransmitting relay if the decision variable corresponding to the relay is larger than the threshold, otherwise, the destination marks it as a silent relay. Then the destination combines the signals from the retransmitting relays and from the source to make the final decision. The average end-to-end (e2e) bit-error-rate (BER) is derived in a closed-form expression for single-relay and two-relay networks. The problems of selecting optimal thresholds or jointly optimal thresholds and power allocation to minimize the average BER are investigated. Simulation results are provided to validate our analysis. Compared to the previously proposed piecewise-linear (PL) detection scheme, our proposed scheme yields a superior performance under a wide range of channel conditions when optimal thresholds or jointly optimal thresholds and power allocation are employed.
Open science challenges, benefits and tips in early career and beyond

The movement towards open science is a consequence of seemingly pervasive failures to replicate previous research. This transition comes with great benefits but also significant challenges that are likely to affect those who carry out the research, usually early career researchers (ECRs). Here, we describe key benefits, including reputational gains, increased chances of publication, and a broader increase in the reliability of research. The increased chances of publication are supported by exploratory analyses indicating null findings are substantially more likely to be published via open registered reports in comparison to more conventional methods. These benefits are balanced by challenges that we have encountered and that involve increased costs in terms of flexibility, time, and issues with the current incentive structure, all of which seem to affect ECRs acutely. Although there are major obstacles to the early adoption of open science, overall open science practices should benefit both the ECR and improve the quality of research. We review 3 benefits and 3 challenges and provide suggestions from the perspective of ECRs for moving towards open science practices, which we believe scientists and institutions at all levels would do well to consider.
Species coexistence: macroevolutionary relationships and the contingency of historical interactions

Evolutionary biologists since Darwin have hypothesized that closely related species compete more intensely and are therefore less likely to coexist. However, recent theory posits that species diverge in two ways: either through the evolution of ‘stabilizing differences’ that promote coexistence by causing individuals to compete more strongly with conspecifics than individuals of other species, or through the evolution of ‘fitness differences’ that cause species to differ in competitive ability and lead to exclusion of the weaker competitor. We tested macroevolutionary patterns of divergence by competing pairs of annual plant species that differ in their phylogenetic relationships, and in whether they have historically occurred in the same region or different regions (sympatric versus allopatric occurrence). For sympatrically occurring species pairs, stabilizing differences rapidly increased with phylogenetic distance. However, fitness differences also increased with phylogenetic distance, resulting in coexistence outcomes that were unpredictable based on phylogenetic relationships. For allopatric species, stabilizing differences showed no trend with phylogenetic distance, whereas fitness differences increased, causing coexistence to become less likely among distant relatives. Our results illustrate the role of species' historical interactions in shaping how phylogenetic relationships structure competitive dynamics, and offer an explanation for the evolution of invasion potential of non-native species.
Numerical study of hemodynamics in brain aneurysms treated with flow diverter stents using porous medium theory

Abstract Conventional approaches of implementing computational fluid dynamics to study aneurysmal hemodynamics after treatment with a flow diverter stent are computationally expensive. Cumbersome meshing and lengthy simulation runtimes are common. To address these issues, we present a novel volume penalization method that considers flow diverters as heterogeneous porous media. The proposed model requires a considerably smaller number of mesh elements, leading to faster simulation runtimes. Three patient-specific aneurysms were virtually treated with flow diverters and aneurysmal hemodynamics were simulated. The results of the virtual deployments including aneurysmal hemodynamics were compared to corresponding results from conventional approaches. The comparisons showed that the proposed approach led to 9.12 times increase in the speed of simulations on average. Further, aneurysmal kinetic energy and inflow rate metrics for the proposed approach were consistent with those from conventional approaches, differing on average by 3.52% and 3.78%, respectively.
Sequence analysis of the mouse IRBP gene and cDNA

Purpose. To understand the structure of the mouse interphotoreceptor retinoid-binding protein (IRBP) gene and to compare the predicted primary structure within each repeat of IRBP with its relatives. To compare the levels of expression of IRBP RNA in normal and knockout mice. Methods. The DNA sequence was determined by subcloning restriction fragments of the IRBP gene from 129/Sv P1 clones. Primers were designed to utilize the walking approach. Additional sequences were obtained by PCR amplification from genomic DNA and direct sequencing of products. Mouse retina RNA was subjected to reverse transcription coupled to PCR and the accumulation of double stranded DNA product was monitored with SYBR Green. The PCR primers flanked Intron C, to avoid the analysis of contaminating genomic DNA. Results. Altogether a contig was assembled with a final length of about 14.4kb. The mouse gene structure is similar to the pattern of exons and introns in the bovine and human genes, with a long first exon encoding most of the protein. The splice site boundaries closely match consensus sequences and the exons appear to be identically placed among the three species (bovine, human, and mouse). A region containing a repeated sequence of low complexity is located about 1.75 to 1.4kb upstream of the transcription start site. A second region containing another low complexity repeat is found in Intron C close to the end of Exon 3. A limited number of weak consensus polyadenylation signals in the 3' region suggest at least three different transcription terminators that apparently give rise to the previously known mouse IRBP mRNAs. The mRNA for IRBP was detected in normal and part of the mRNA was detected in the IRBP knockout mouse, consistent with previous observations. The level of the IRBP mRNA remnant was reduced about 10 fold in the knockout mouse, also consistent with the previously reported absence of Repeat 4 immunoreactivity. Conclusions. The strong conservation in intron-exon positions, gene structure, and protein sequence among mammals supports an important biological role for these signals and for the IRBP protein in vision. Low levels of aberrant IRBP mRNA in the knockout mouse are consistent with no immunologically detectable Repeat 4 protein in this mouse.
Good hemostatic effect of platelets stored at 4°C in an in vitro model of massive blood loss and thrombocytopenia

Abstract This study compared the corrective effects of storage of platelets at 4°C and at 22°C in an in vitro model of massive blood loss and thrombocytopenia to provide an experimental basis for the storage of platelets for clinical applications. In vitro model of massive blood loss and thrombocytopenia were constructed by the in vitro hemodilution method and cell washing method. Using storage of platelets at 4°C (1, 3, 5, 7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the coagulation condition of the different models, by thromboelastography and by routine blood indices. &U2460;Platelets stored at 4°C (1, 3, 5,7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the in vitro model of massive blood loss. Platelet count results improved from 17 to 27 × 109/L to greater than 120 × 109/L for 4°C storage, and 20 to 27 × 109/L to greater than 120 × 109/L for 22°C storage. Thromboelastography maximum amplitude (TEG-MA) results improved from 8.8 to 15.4 mm to greater than 43 mm for 4°C storage, and 12.2 to 14.4 mm to greater than 44.8 mm for 22°C storage. Thromboelastography reaction time values decreased from 9.9–24.9 minutes to 3.8–5.5 minutes for 4°C storage, and 9.9–22.7 minutes to 4.3–4.5 minutes for 22°C storage. &U2461;Platelets stored at 4°C (1, 3, 5,7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the in vitro model of thrombocytopenia. Platelet count results improved from 12 to 34 × 109/L to greater than 99 × 109/L for 4°C storage, and 12 to 34 × 109/L to greater than 120 × 109/L for 22°C storage. TEG-MA results improved from 21.4 to 32.1 mm to greater than 49.1 mm for 4°C storage, and 21.4 to 31.6 mm to greater than 50.5 mm for 22°C storage. Platelets stored at 4°C and 22°C have the same correcting effect for 1, 3, and 5 days. Platelets stored at 4°C for 7 to 14 days have similarly hemostatic effect on the in vitro model of massive blood loss and thrombocytopenia.
Abstract—this Paper Presents a Real-time Defect Detection

algorithm for high-speed steel bar in coil. Because the target speed is very high, proposed algorithm should process quickly the large volumes of image for real-time processing. Therefore, defect detection algorithm should satisfy two conflicting requirements of reducing the processing time and improving the efficiency of defect detection. To enhance performance of detection, edge preserving method is suggested for noise reduction of target image. Finally, experiment results show that the proposed algorithm guarantees the condition of the real-time processing and accuracy of detection.