Miracle-dz/UniFAR-FTUs huggingface dataset:
Antisense mRNA-Mediated Bacteriophage Resistance in Lactococcus lactis subsp. lactis

Resistance to a broad class of isometric bacteriophages that infect strains of Lactococcus lactis has been engineered into a dairy starter by expression of antisense mRNA targeted against a conserved bacteriophage gene. Maximum protection is obtained only when the entire 1,654-bp coding sequence for a 51-kDa protein is positioned in the antisense orientation with respect to a promoter sequence that functions in L. lactis subsp. lactis. Expression of the antisense mRNA results in more than 99% reduction of the total number of PFU. Plaques that do form are characterized by their relatively small size and irregular shape. A variety of truncated genes, including the open reading frame expressed in the sense orientation, fail to provide any significant measure of resistance as compared with that of the intact open reading frame. Southern hybridization with probes specific for the conserved region reveal that the [ill] plasmid constructs are maintained despite the presence of a large complement of other indigenous plasmids. Strains harboring the antisense mRNA plasmid construct grow and produce acid at a rate equivalent to that of the host strain alone, suggesting that antisense expression is not deleterious to normal cellular metabolism.
A new family of soft corals (Anthozoa, Octocorallia, Alcyonacea) from the aphotic tropical eastern Pacific waters revealed by integrative taxonomy

The new family Aquaumbridae is described based on distinct morphological characters and supported by a molecular phylogenetic analysis. Aquaumbridae is similar to Nidaliidae, Nepththeidae and Alcyoniidae in having arborescent colonies and lacking axis structure or stolons, but differs from them in having very different sclerite composition and having conspicuous transparent jelly-like lobes. Phylogenetic analysis of two mitochondrial genes, ND2 and mtMutS, strongly supports its placement in a separated clade. Herein we describe Aquaumbra klapferi sp. nov., gen. nov. in the new family. The organisms were obtained from the seamounts, ridges and canyons out of the insular shelf of Isla del Coco, Costa Rica, down to 400 m depth. The new species represents the first discovery of a soft coral in an eastern Pacific oceanic island, and provides hints of the biodiversity of the largely unexplored deep waters of the tropical eastern Pacific.
Sex on the internet: Observations and implications for internet sex addiction

Some academics claim that social pathologies referred to as technological addictions are beginning to surface in cyberspace. One related area that deserves further examination is the concept of sex addiction and its relationship with excessive Internet usage. This article therefore overviews (a) sex‐related uses of the Internet, (b) the concept of “Internet addiction” in relation to excessive sexual behavior, (c) cybersex and cyber‐relationships, (d) cyber‐relationship typologies, (e) the claims made for Internet sex addiction, and (f) the empirical data relating to Internet sex addiction. It is concluded that Internet sex is a new medium of expression where factors such as perceived anonymity and disinhibition may increase participation. It is also argued that although the amount of empirical data is small, Internet sex addiction exists.
The impact of an educational program based on BASNEF model on the selection of a contraceptive method in women

Background: Quality of services, making communications with target groups, and educating them are among the most important success factors in implementation of family planning programs. Provision of public access to contraception and related methods, counseling services, and paying attention to social specification and cultures are important in promotion of service quality. With regard to applicability of health education theories and models, the present study aimed to find the impact of an educational program based on BASNEF model to choose contraceptive methods in women referring to health care centers in Minoodasht in 2012. Materials and Methods: This is a quasi-experimental study. Data were collected using BASNEF questionnaire by the researcher from women referring to health care centers in two groups of study and control (n = 100 in each group). Educational intervention (in the form of four educational 1-h sessions once a week during 1 month and two additional review sessions) was conducted in the form of group and face-to-face discussions and educational booklets were distributed. Data were analyzed by Chi-square, analysis of covariance (ANCOVA), paired t-test, and t-test through SPSS version 14. Results: After intervention, mean score of knowledge was significantly higher in the study group compared to control (P < 0.003). Intervention led to a better attitude in the study group (P < 0.0001). Mean score of subjective norms and enabling factors after intervention was significantly higher in the study group compared to control (P < 0.0001). The change in practice had a significant increase in the study group (54%) compared to control (9%). Conclusions: BASNEF-based educational intervention was effective in increasing women's knowledge, attitude, and practice. As family and educational facilities are among the influencing factors on contraceptive method selection, interventional planning is hoped to be based on educational model.
Developing the Moral Integrity of College Sport through Commercialism

Developing the Moral Integrity of College Sport through Commercialism Despite criticisms that commercialism corrupts college sports (Duderstadt, 2003, Roberts 2008, Zimbalist 2006), commercialism, if executed appropriately can strengthen and develop the moral integrity of sport. A utilitarian approach to the commercialization of sport can be used to strengthen its moral integrity. From a utilitarian standpoint, John Stuart Mill's greatest happiness principle and Jeremy Bentham's hedonic calculus can be used to help determine specific approaches to the commercialization of sport. The interests of the sporting community including sport participants, coaches, administrators, fans, and community members, must be considered when commercializing sport to a moral end. Thoroughly understanding a morally grounded mission in a sport organization is a prerequisite to the effective negotiation of terms of commercial agreements that mutually support the mission of the commercial entity and sport organization. The commercialization of sport includes but is not limited to television contracts, venue signage, licensing of merchandise, and corporate sponsorships. Identifying a professional "fit" between the sport organization and commercial entity is imperative. The use of common sense, and experience as outlined by Mill and Bentham (as cited in Beauchamp 1982) and understanding human nature as outlined by Hume (1739/1964) are useful when attempting to determine how particular commercialism efforts may consciously or subconsciously develop or reduce the moral integrity of sport. Beyond sport, the influences and risks of commercialism can be understood by observing its effect on non-sport organizations. Threats to the moral integrity of sport arise when entering into revenue generating commercial agreements. Sport's overreliance on revenue from a commercial entity is a factor that can potentially cause deviation from a sport organization's morally based mission. Excessive expansion and lavish funding of sport organizations can contribute to overreliance on revenue from commercial entities. Personal greed can also play a role in detracting from the moral integrity of the mission.
Decoherence and fidelity in ion traps with fluctuating trap parameters

We consider two different kinds of fluctuations in an ion trap potential: external fluctuating electrical fields, which cause statistical movement (wobbling) of the ion relative to the center of the trap, and fluctuations of the spring constant, which an due to fluctuations of the ac component of the potential applied in the Paul trap for ions. We write down master equations for both cases and, averaging out the noise, obtain expressions for the heating of the ion. We compare our results to previous results for far-off resonance optical traps and heating in ion traps. The effect of fluctuating external electrical fields for a quantum gate operation (controlled-NOT) is determined and the fidelity for that operation derived. [S1050-2947(99)06005-9].
A Self-Representation Induced Classifier

Almost all the existing representation based classifiers represent a query sample as a linear combination of training samples, and their time and memory cost will increase rapidly with the number of training samples. We investigate the representation based classification problem from a rather different perspective in this paper, that is, we learn how each feature (i.e., each element) of a sample can be represented by the features of itself. Such a self-representation property of sample features can be readily employed for pattern classification and a novel self-representation induced classifier (SRIC) is proposed. SRIC learns a self-representation matrix for each class. Given a query sample, its self-representation residual can be computed by each of the learned self-representation matrices, and classification can then be performed by comparing these residuals. In light of the principle of SRIC, a discriminative SRIC (DSRIC) method is developed. For each class, a discriminative self-representation matrix is trained to minimize the self-representation residual of this class while representing little the features of other classes. Experimental results on different pattern recognition tasks show that DSRIC achieves comparable or superior recognition rate to state-of-the-art representation based classifiers, however, it is much more efficient and needs much less storage space.
Cytotoxin production by Helicobacter pylori from patients with upper gastrointestinal tract diseases

A cytotoxin produced by some Helicobacter pylori strains has recently been identified. The cytotoxin induces intracellular vacuolization of cultured cells. The aim of the present study was to examine the frequency of occurrence of cytotoxin-producing strains of H. pylori from subjects with upper gastrointestinal disease including nonulcer dyspepsia, gastric and duodenal ulcer disease, gastroesophageal reflux disease, and gastric cancer. Broth culture filtrates of clinical isolates of H. pylori recovered from 175 patients were used to inoculate Vero and HeLa cell monolayers for the detection of vacuolating cytotoxin activity. The results obtained demonstrated that the highest percentage of strains producing cytotoxin were found in subjects with peptic ulcer disease (gastric ulcer, 65%; duodenal ulcer, 66%; P < 0.01 compared with nonulcer dyspepsia, 38%). Of the 11 patients with gastroesophageal reflux disease, 4 of 5 patients in this group who had esophageal ulcers, were found to be infected with strains that produced cytotoxin. Three of the four patients with carcinoma of the stomach were also found to be infected with cytotoxic strains of H. pylori. With increasing severity of mucosal damage in subjects with a normal upper gastrointestinal tract, macroscopic gastritis, duodenitis, and peptic ulceration, there were corresponding increase in the proportion of strains producing cytotoxin; these increases were 32, 46, 50, and 66%, respectively. H. pylori strains from subjects with ulcer disease commonly produced vacuolating cytotoxin, suggesting that it may be a virulence factor in the pathogenesis of peptic ulcer disease.
Potential of microneedle-assisted micro-particle delivery by gene guns: a review

Abstact Context: Gene guns have been used to deliver deoxyribonucleic acid (DNA) loaded micro-particle and breach the muscle tissue to target cells of interest to achieve gene transfection. Objective: This article aims to discuss the potential of microneedle (MN) assisted micro-particle delivery from gene guns, with a view to reducing tissue damage. Methods: Using a range of sources, the main gene guns for micro-particle delivery are reviewed along with the primary features of their technology, e.g. their design configurations, the material selection of the micro-particle, the driving gas type and pressure. Depending on the gene gun system, the achieved penetration depths in the skin are discussed as a function of the gas pressure, the type of the gene gun system and particle size, velocity and density. The concept of MN-assisted micro-particles delivery which consists of three stages (namely, acceleration, separation and decoration stage) is discussed. In this method, solid MNs are inserted into the skin to penetrate the epidermis/dermis layer and create holes for particle injection. Several designs of MN array are discussed and the insertion mechanism is explored, as it determines the feasibility of the MN-based system for particle transfer. Results: This review suggests that one of the problems of gene guns is that they need high operating pressures, which may result in direct or indirect tissue/cells damage. MNs seem to be a promising method which if combined with the gene guns may reduce the operating pressures for these devices and reduce tissue/cell damages. Conclusions: There is sufficient potential for MN-assisted particle develivery systems.
On the theory and practice of critical pragmatism: Deliberative practice and creative negotiations

Critical pragmatism provides a line of analysis and imagination that might contribute both to academic planning theory and to engaged planning practices as well. To do so, it must build upon, and develop more politically, Donald Schön’s seminal work on reflective practice. It must help students of planning think critically about outcomes as well as processes, about institutional and process designs, about power and performance. It must resonate experientially with perceptions of change-oriented practitioners facing complex multi-party “problems” characterized by distrust, anger, strategic behavior, poor information, and inequalities of power. Not least of all, a critical pragmatism must—and can—help students of planning reconstruct possibilities where others might initially perceive or presume impossibilities.
Antagonism between Cry1Ac1 and Cyt1A1 Toxins ofBacillus thuringiensis

ABSTRACT Most strains of the insecticidal bacterium Bacillus thuringiensis have a combination of different protoxins in their parasporal crystals. Some of the combinations clearly interact synergistically, like the toxins present in B. thuringiensis subsp. israelensis. In this paper we describe a novel joint activity of toxins from different strains ofB. thuringiensis. In vitro bioassays in which we used pure, trypsin-activated Cry1Ac1 proteins from B. thuringiensissubsp. kurstaki, Cyt1A1 from B. thuringiensissubsp. israelensis, and Trichoplusia niBTI-Tn5B1-4 cells revealed contrasting susceptibility characteristics. The 50% lethal concentrations (LC50s) were estimated to be 4,967 of Cry1Ac1 per ml of medium and 11.69 ng of Cyt1A1 per ml of medium. When mixtures of these toxins in different proportions were assayed, eight different LC50s were obtained. All of these LC50s were significantly higher than the expected LC50s of the mixtures. In addition, a series of bioassays were performed with late first-instar larvae of the cabbage looper and pure Cry1Ac1 and Cyt1A1 crystals, as well as two different combinations of the two toxins. The estimated mean LC50 of Cry1Ac1 was 2.46 ng/cm2 of diet, while Cyt1A1 crystals exhibited no toxicity, even at very high concentrations. The estimated mean LC50s of Cry1Ac1 crystals were 15.69 and 19.05 ng per cm2 of diet when these crystals were mixed with 100 and 1,000 ng of Cyt1A1 crystals per cm2 of diet, respectively. These results indicate that there is clear antagonism between the two toxins both in vitro and in vivo. Other joint-action analyses corroborated these results. Although this is the second report of antagonism between B. thuringiensis toxins, our evidence is the first evidence of antagonism between toxins from different subspecies of B. thuringiensis (B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. israelensis) detected both in vivo and in vitro. Some possible explanations for this relationship are discussed.
Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)

Histone acetylation and deacetylation at the N-terminus of histone tails play crucial roles in the regulation of eukaryotic gene activity. Histone acetylation and deacetylation are catalyzed by histone acetyltransferases and histone deacetylases (HDACs), respectively. A growing number of studies have demonstrated the importance of histone deacetylation/acetylation on genome stability, transcriptional regulation, development and response to stress in Arabidopsis. However, the biological functions of HDACs in tomato have not been investigated previously. Fifteen HDACs identified from tomato (Solanum lycopersicum) can be grouped into RPD3/HDA1, SIR2 and HD2 families based on phylogenetic analysis. Meanwhile, 10 members of the RPD3/HDA1 family can be further subdivided into four groups, namely Class I, Class II, Class III, and Class IV. High similarities of protein sequences and conserved domains were identified among SlHDACs and their homologs in Arabidopsis. Most SlHDACs were expressed in all tissues examined with different transcript abundance. Transient expression in Arabidopsis protoplasts showed that SlHDA8, SlHDA1, SlHDA5, SlSRT1 and members of the HD2 family were localized to the nucleus, whereas SlHDA3 and SlHDA4 were localized in both the cytoplasm and nucleus. The difference in the expression patterns and subcellular localization of SlHDACs suggest that they may play distinct functions in tomato. Furthermore, we found that three members of the RPD3/HDA1 family, SlHDA1, SIHDA3 and SlHDA4, interacted with TAG1 (TOMATO AGAMOUS1) and TM29 (TOMATO MADS BOX29), two MADS-box proteins associated with tomato reproductive development, indicating that these HDACs may be involved in gene regulation in reproductive development.
Effects of heparin, aspirin and a synthetic platelet glycoprotein IIb-IIIa receptor antagonist (Ro 43-5054) on coronary artery reperfusion and reocclusion after thrombolysis with tissue-type plasminogen activator in the dog.

Adjuncts to thrombolytic agents have improved coronary patency and prevented early reocclusion after thrombolysis in acute myocardial infarction. The aim of this study was to compare in a canine thrombolysis model the effects of Ro 43-5054 = N-(N-[N-(p-amidinobenzoyl)-beta-alanyl]-L-alpha-aspartyl)-3- phenyl-L-alanine-trifluor-acetate, a new glycoprotein IIb-IIIa receptor antagonist with aspirin or heparin. Six groups of 10 dogs each were studied. A platelet-rich coronary thrombus was induced in open-chest dogs by electrical stimulation. In addition to recombinant tissue-type plasminogen activator (30 micrograms/kg.min during 60 min), the dogs received 1) saline, 2) heparin 200 U/kg + 50 U/kg.hr i.v. , 3) aspirin 10 mg/kg i.v. , 4) heparin+aspirin, 5) Ro 43-5054 (3 micrograms/kg.min) and 6) heparin+Ro 43-5054. The overall reperfusion rate was 70% (range, 60-90%) and comparable in all the six groups. During the 120-min observation period, episodes of reocclusion were observed in the absence of antiplatelet therapy (group 1 and 2) irrespective of heparin treatment. Aspirin prevented coronary reocclusion in half of the reperfused dogs (group 3 and 4). However, after reinforcement of the thrombogenic stimulus, 80% of the reperfused dogs treated with aspirin showed reocclusion, whereas none of them reoccluded when treated with Ro 43-5054. Thus, inhibition of platelet activation by the selective, nonpeptidic glycoprotein IIb-IIIa receptor antagonist Ro 43-5054, although without effect on the time to reperfusion, better protected than aspirin against early reocclusion after thrombolytic therapy.
Machine Learning for Intelligent Systems

Recent research in machine learning has focused on supervised induction for simple classification and reinforcement learning for simple reactive behaviors. In the process, the field has become disconnected from AI's original goal of creating complete intelligent agents. In this paper, I review recent work on machine learning for planning, language, vision, and other topics that runs counter to this trend and thus holds interest for the broader AI research community. I also suggest some steps to encourage further research along these lines.
Model-Based Adaptive Iterative Hard Thresholding Compressive Sensing in Sensor Network for Volcanic Earthquake Detection

Recent years have witnessed pilot deployments of inexpensive wireless sensor networks (WSNs) for volcanic eruption detection, where the volcano-seismic signals were collected and processed by sensor nodes. However, it is faced with the limitation of energy resources and the transmission bottleneck of sensors in WSN. In this paper, a Model-Based Adaptive Iterative Hard Thresholding (MAIHT) compressive sensing scheme is developed, where a large number of inexpensive sensors are used to collect fine-grained, real-time volcano-seismic signals while a small number of powerful coordinator nodes process and pick arrival times of primary waves (i.e., P-phases). The paper contribution is two-fold. Firstly, a sparse measurement matrix with theoretical analysis of its restricted isometry property (RIP) is designed to simplify the acquisition process, thereby reducing required storage space and computational demands in sensors. Secondly, a compressive sensing reconstruction algorithm with theoretical analysis of its error bound is presented. Experimental results based on real volcano-seismic data collected from a volcano show that our method can recover the original seismic signal and achieve accurate P-phase picking based on the reconstructed seismic signal.
Cutaneous metastases from hepatocellular carcinoma

A 52‐year‐old South‐east Asian came in with complaints of an ulcerated, profusely bleeding, painful, 7‐cm cutaneous mass over his left scapula. This actively bleeding mass had a dusky vascular appearance and a boggy central core ( Fig. 1 ). The patient first noticed the mass 6 months before seeking medical attention. At that time it was a centimeter in diameter.