Miracle-dz/UniFAR-FTUs huggingface dataset:
Invasive Breast Cancer with HER2 ≥4.0 and <6.0: Risk Classification and Molecular Typing by a 21-Gene Expression Assay and MammaPrint Plus BluePrint Testing

Purpose To investigate the HER2 status and clinicopathological features in invasive breast cancer with HER2 ≥4.0 and <6.0, which has always been controversial. Methods Forty breast cancer cases with HER2 ≥4.0 and <6.0 by fluorescence in situ hybridization (FISH) were collected and classified into two groups based on the HRE2/CEP17 ratio (Group A: ≥2.0, n=22; Group B: <2.0, n=18). Clinicopathological characteristics, HER2 status, risk classification, and molecular typing were further analyzed and compared by 21-Gene expression assay and MammaPrint plus BluePrint test. Results The majority of cases in both groups were invasive carcinoma (NOS), with histological grade II, HR+, Ki-67 ≥20%, HER2 2+, and a high risk of recurrence, although younger patients and lymph node metastases were more common in Group A. Surprisingly, all HR+ breast cancers in both groups were classified as luminal-type, HR− cases were all basal-type or unknown, and the index of HER2 in all cases was <0.000 using the BluePrint test, which indicated that HER2 status should be negative. Furthermore, the level of HER2 mRNA expression in all cases of both groups was <10.7, which was defined as HER2 negative by the 21-Gene expression assay. In addition, 10 patients of Group A received anti-HER2 neoadjuvant therapy; only one patient with HR- achieved Grade 5 based on the Miller-Payne system, whereas none of the patients achieved pathological complete response (pCR) based on the Residual Cancer Burden system. Conclusion Group A breast cancer, which has always been unquestionably diagnosed as HER2 amplification, was more likely to be HER2 negative and derived less benefit from anti-HER2 neoadjuvant chemotherapy. Group A breast cancer should be distinguished from classical HER2-positive breast cancers when assessing HER2 FISH, and a larger cohort of Group A patients should be included in further studies.
Effects of Increasing Levels of Total Tannins on Intake, Digestibility, and Balance of Nitrogen, Water, and Energy in Hair Lambs

Simple Summary Sheep farming plays a prominent role in the agriculture and economy of tropical regions, including the Brazilian semi-arid region. Rural producers have adopted Mimosa tenuiflora hay as a feed option for sheep and goats due to its prevalence in these areas. However, it is important to point out that Mimosa tenuiflora hay contains tannins, compounds with antinutritional and astringent properties. Therefore, it is crucial to understand the interaction of natural tannins derived from legumes with a ruminal metabolism in terms of energy and water balance. The inclusion of Mimosa tenuiflora hay as a source of tannins in the diet of hair lambs can potentially have positive effects on animal consumption. Abstract This study aims to evaluate the effects of increasing tannin levels from Mimosa tenuiflora hay on the intake, digestibility, and balance of nitrogen (N), water, and energy in hair lambs. Thirty-two Santa Ines lambs, at an average age of 150 days and body weight of 26.75 ± 2.29 kg, were randomly assigned to four treatments in a completely randomized design. The treatments consisted of four diets: a control diet, tannin-free, and three diets with increasing levels of total tannin, 26.2, 52.4, and 78.6 g tannin/kg dry matter (DM). Including the total tannins in the lambs’ diet led to a quadratic increase in the intake of nutrients, N-retention (g/day), water intake, water absorption and retention, energy intake, and energy excretion in feces and gases. However, the digestibility of crude protein, neutral and acid detergent fibers, and total carbohydrates decreased. It was observed that there is a correlation between the variable nutrient digestibility and N-ingested and the N-absorbed, N-urinary, and N-retained. However, the N-excreted in feces did not correlate with any of the variables studied. It is recommended to include 33 g/kg DM of total natural tannins from Mimosa tenuiflora hay in the diet of hair lambs, as it improves intake, energy balance, dietary N, and body water composition while reducing the excretion of N-urinary and gas emissions to the environment.
AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAN AIR RANTING BUAH PARIJOTO (Medinilla speciosa Blume) DENGAN PEREDAMAN RADIKAL BEBAS DPPH

Radikal bebas adalah molekul yang tidak mempunyai pasangan elektron di kulit bagian luar menyebabkan bersifat sangat reaktif. Oksidasi akibat radikal bebas dapat dicegah dan dihambat oleh substansi yaitu antioksidan. Antioksidan dapat menghambat kerusakan sel akibat radikal bebas. Antioksidan alami didapatkan dari tanaman atau buah-buahan. Indonesia memiliki daerah hutan tropis yang sangat besar, tetapi sebagian besar dari spesies hayati hutan tropis belum dimanfaatkan sepenuhnya. Salah satu kearifan lokal yang digunakan masyarakat Desa Colo kecamatan Dawe Kabupaten Kudus yaitu menggunakan tanaman parijoto (Medinilla speciosa Blume). Tanaman parijoto mengandung saponin, flavonoid dan tanin pada ranting buahnya. Senyawa flavonoid memiliki kemampuan mencegah kerusakan pada sel ? pancreas, sehingga merupakan salah satu fungsi dari antioksida. Tujuan dilakukan penelitian ini adalah untuk mengetahui aktivitas antioksidan fraksi etil asetat dan air ranting buah parijoto (Medinilla speciosa Blume) dengan peredaman radikal bebas DPPH. Hasil dari penelitian didapatkan nilai IC50 pada fraksi etil asetat sebesar 257,25 ppm sedangkan fraksi air ranting parijoto sebesar 338,17 ppm yang termasuk golongan antioksidan yang lemah. Sedangkan IC50 pembanding kuersetin sebesar 6,09 ppm yang mempunyai aktivitas antioksidan sangat kuat.
The Comparison of the 3D graph for the energy-equal of LEACH-Mobile

In this paper, propose an algorithm to improve network lifetime by equally consuming energy of LEACH - Mobile sensor nodes. LEACH is one of energy efficient protocols. However, we did not consider the mobility of nodes. Therefore, the transmission reception success rate of the moving data is reduced. LEACH-Mobile is a protocol that has improved the drawbacks of these LEACH. However, since LEACH-Mobile has a larger number of data packets and consumes more energy than LEACH, it has a disadvantage that the lifetime of the network is short. In order to improvement these disadvantage, Based on the average of the remaining energy of the node, cluster heads are elected with a number of nodes whose energies are larger than the average of the remaining energy from the member nodes. After that, by trying to increase the lifetime of the network by equalizing the remaining energy. In to confirm whether improve the lifetime of the network, In this paper, the number of nodes and the position of all nodes are varied for each specific round, the rest energy is equalized, and the algorithm which uniformly selected the cluster head is compared with LEACH.
Hybrid machine-learning framework for volumetric segmentation and quantification of vacuoles in individual unlabeled yeast cells using holotomography

The precise, quantitative evaluation of intracellular organelles in three-dimensional (3D) imaging data poses a significant challenge due to the inherent constraints of traditional microscopy techniques, the requirements of the use of exogenous labeling agents, and existing computational methods. To counter these challenges, we present a hybrid machine-learning framework exploiting correlative imaging of 3D quantitative phase imaging with 3D fluorescence imaging of labeled cells. The algorithm, which synergistically integrates a random-forest classifier with a deep neural network, is trained using the correlative imaging data set, and the trained network is then applied to 3D quantitative phase imaging of unlabeled cell data. We applied this method to unlabeled live budding yeast cells. The results revealed precise segmentation of vacuoles inside individual yeast cells, and also provided quantitative evaluations of biophysical parameters, including volumes, concentration, and dry masses of automatically segmented vacuoles.
“Green” Pericyclic Reactions Assisted by Ionic Liquids

Ionic liquids (ILs) are compounds that consist exclusively of ions. These compounds, which can be considered as salts, feature low melting temperatures (generally below 100°C), at which other salts are solids. The cations in these compounds are of organic type and some of the most common structures are heterocyclic such as imidazolium (1), pyridinium (2), pyrazolium (3) and pyperidinium (4), or may also be formed by non-cyclic, heteroatom-containing cations such as ammonium (5) and phosphonium (6) (Figure 1) [1].
Cardiorespiratory fitness is associated with hippocampal resting state connectivity in women newly diagnosed with breast cancer

Background: Breast cancer and its treatment are associated with aberrant patterns of resting state functional connectivity (rsFC) between the hippocampus and several areas of the brain, which may account for poorer cognitive outcomes in patients. Higher cardiorespiratory fitness (CRF) has been associated with enhanced rsFC and cognitive performance; however, these associations have not been well studied in breast cancer. We examined the relationship between CRF, rsFC of the hippocampus, and cognitive performance among women newly diagnosed with breast cancer. Methods: Thirty-four postmenopausal women newly diagnosed with Stage 0-IIIa breast cancer (Mage = 63.59 ± 5.73) were enrolled in a 6-month randomized controlled trial of aerobic exercise vs. usual care. During baseline assessments, participants completed functional brain imaging, a submaximal CRF test, and cognitive testing. Whole-brain, seed-based analyses were used to examine the relationship between CRF and hippocampal rsFC, with age, years of education, and framewise displacement included as covariates. Cognition was measured with a battery of validated neurocognitive measures, reduced to seven composite factors. Results: Higher CRF was positively associated with greater rsFC of the hippocampus to a cluster within the dorsomedial and dorsolateral frontal cortex (z-max = 4.37, p = 0.003, cluster extent = 1,020 voxels). Connectivity within cluster peaks was not significantly related to cognitive factors (all ps > 0.05). Discussion: CRF was positively associated with hippocampal rsFC to frontal cortex structures, comprising a network of regions commonly suppressed in breast cancer. Future longitudinal research is needed to explore whether baseline rsFC predicts long-term cognitive resilience in breast cancer.
A Scoping Review of Research Exploring Working Life Practices of People with Disabilities During the COVID-19 Pandemic

There have been numerous research studies stating the fact that the pandemic affected people with disabilities’ working lives. Less research has been conducted on how people with disabilities coped with and learned from these challenges. This scoping review maps research conducted in the field of disability research and multidisciplinary research, published from the outbreak of the pandemic until October 31, 2022. The focus is on how people with disabilities adapted their working lives to the conditions caused by the COVID-19 pandemic and what working practices and strategies they applied to manage the situation. From an extensive search in bibliographic databases, eight research articles were identified. The review results reveal both challenges and new openings for the working life of people with disabilities post-pandemic. Implications for future research are identified, addressing intersectionality, hybrid work environments, digital gaps and increased participation of people with disabilities in research.
Formulation and Evaluation of a Self-Microemulsifying Drug Delivery System of Raloxifene with Improved Solubility and Oral Bioavailability

Poor aqueous solubility and dissolution limit the oral bioavailability of Biopharmaceutics Classification System (BCS) class II drugs. In this study, we aimed to improve the aqueous solubility and oral bioavailability of raloxifene hydrochloride (RLX), a BCS class II drug, using a self-microemulsifying drug delivery system (SMEDDS). Based on the solubilities of RLX, Capryol 90, Tween 80/Labrasol ALF, and polyethylene glycol 400 (PEG-400) were selected as the oil, surfactant mixture, and cosurfactant, respectively. Pseudo-ternary phase diagrams were constructed to determine the optimal composition (Capryol 90/Tween 80/Labrasol ALF/PEG-400 in 150/478.1/159.4/212.5 volume ratio) for RLX-SMEDDS with a small droplet size (147.1 nm) and stable microemulsification (PDI: 0.227). Differential scanning calorimetry and powder X-ray diffraction of lyophilized RLX-SMEDDS revealed the loss of crystallinity, suggesting a molecularly dissolved or amorphous state of RLX in the SMEDDS formulation. Moreover, RLX-SMEDDS exhibited significantly higher saturation solubility and dissolution rate in water, simulated gastric fluid (pH 1.2), and simulated intestinal fluid (pH 6.8) than RLX powder. Additionally, oral administration of RLX-SMEDDS to female rats resulted in 1.94- and 1.80-fold higher area under the curve and maximum plasma concentration, respectively, than the RLX dispersion. Collectively, our findings suggest SMEDDS is a promising oral formulation to enhance the therapeutic efficacy of RLX.
MicroRNAs miR-16 and miR-519 control meningioma cell proliferation via overlapping transcriptomic programs shared with the RNA-binding protein HuR

Introduction Meningiomas are the most common type of primary central nervous system tumors. In about 80% cases, these tumors are benign and grow very slowly, but the remainder 20% can unlock higher proliferation rates and become malignant. In this study we examined two miRs, miR-16 and miR-519, and evaluated their role in tumorigenesis and cell growth in human meningioma. Methods A cohort of 60 intracranial grade 1 and grade 2 human meningioma plus 20 healthy meningeal tissues was used to quantify miR-16 and miR-519 expressions. Cell growth and dose-response assays were performed in two human meningioma cell lines, Ben-Men-1 (benign) and IOMM-Lee (aggressive). Transcriptomes of IOMM-lee cells were measured after both miR-mimics transfection, followed by integrative bioinformatics to expand on available data. Results In tumoral tissues, we detected decreased levels of miR-16 and miR-519 when compared with arachnoid cells of healthy patients (miR-16: P=8.7e-04; miR-519: P=3.5e-07). When individually overexpressing these miRs in Ben-Men-1 and IOMM-Lee, we observed that each showed reduced growth (P<0.001). In IOMM-Lee cell transcriptomes, downregulated genes, among which ELAVL1/HuR (miR-16: P=6.1e-06; miR-519:P=9.38e-03), were linked to biological processes such as mitotic cell cycle regulation, pre-replicative complex, and brain development (FDR<1e-05). Additionally, we uncovered a specific transcriptomic signature of miR-16/miR-519-dysregulated genes which was highly enriched in HuR targets (>6-fold; 79.6% of target genes). Discussion These results were confirmed on several public transcriptomic and microRNA datasets of human meningiomas, hinting that the putative tumor suppressor effect of these miRs is mediated, at least in part, via HuR direct or indirect inhibition.
Risk factors related with avascular necrosis after internal fixation of femoral neck fractures in children: a systematic review and meta-analysis

Background Less than 1% of children develop femoral neck fractures (FNF), making them uncommon. However, they may have dangerous side effects, like avascular necrosis. Even though several risk factors for postoperative avascular necrosis have been identified, there is still debate regarding them. In this investigation, a meta-analysis was performed to examine the potential causes of postoperative avascular necrosis in children with FNF. Methods We conducted a thorough literature search to find risk factors for avascular necrosis (AVN) after internal fixation of pediatric FNF. Until December 2022, we searched several databases, including PubMed, Embase, Cochrane Library, Web of Science, CNKI, Orthosearch, and Sinomed. Software Zotero 6.0 and Stata 17.0 were used to organise and synthesise the data. Finally, a sensitivity and publication bias test was carried out. Results Our study includes a total of 15 case-control studies involving 814 patients. The risk of postoperative AVN increased with age at fracture encounter (95% CI: 0.64–1.88, P = 0.0003), initial fracture displacement (95% CI: 1.87–9.54, P = 0.0005), and poor fracture reduction (95% CI:1.95–22.34, P = 0.0024) were risk factors for postoperative AVN. There was no significant relationship between gender and postoperative AVN (95% CI: 0.52–1.31, P = 0.41). Conversely, Postoperative AVN and reduction methods have no connection with each other (95% CI: 0.77–2.66, P = 0.25), procedure time (95% CI: 0.43–2.99, P = 0.16), or injury mechanism (95% CI: 0.32–2.26, P = 0.75). The incidence of post-operative AVN varies between Delbet fracture types (95% CI: 0.15–0.31, P < 0.0001), with the overall trend being that the incidence of post-operative AVN is highest for type II, lowest for type IV, and close for types I and III, but it is not clear which type of fracture is the independent risk factor. Funnel plots indicate no significant publication bias. Conclusions In line with this study, About 26% of children who underwent surgery for a femoral neck fracture suffered postoperative AVN. The main risk factors for AVN were the child's age, the initial displacement of the fractures, and poorly reduced fractures. The risk of AVN did not significantly correlate with gender, the time of the procedure, reduction methods or the mechanism of injury. The overall trend in the incidence of postoperative AVN for the different Delbet types of fracture is that the incidence of postoperative AVN is highest for type II, lowest for type IV, and close for types I and III, but it is not clear which type of fracture is the independent risk factor.
A virtual boundary method for compressible flow with application to aeroacoustics of compliant trailing-edges

FACULTY OF ENGINEERING AND ENVIRONMENT Aerodynamic and Flight Mechanics Group Doctor of Philosophy by Stefan Christian Schlanderer This work introduces a novel virtual boundary method for compressible flows with application to aeroacoustics. The method is the compressible extension of the boundary data immersion method (BDIM, Maertens & Weymouth (2015)). The BDIM equations for the compressible Navier-Stokes equations are derived and the accuracy of the method for the hydrodynamic representation of solid bodies is demonstrated with challenging test cases relevant to aeroacoustic applications, including a fully turbulent boundary layer flow and a supersonic instability wave. In addition, it was shown that the compressible BDIM is able to accurately represent noise radiation from moving bodies and flow induced noise generation without any penalty in allowable time step. The newly introduced framework was employed to investigate the noise radiation from elastic trailing-edges (TE). A study employing elastic TE extensions for airfoils at angle of attack showed a noise amplification when the structural motion is close to resonance. Furthermore significant effects of the elastic TE extension on the characteristics of the laminar separation bubble on the suction side were found, resulting in a global change of the flow around the airfoil. For that reason, in an attempt to avoid changes in circulation complicating the comparison between rigid and elastic TE, a generic setup featuring a flat plate and a vortex generator was developed to investigate different material parameters for the elastic TE. Excess noise was found to be radiated in frequency bands related to the response of the motion of the elastic structure. However, a noise reduction was observed for certain frequency ranges and structural parameters. The noise reduction was associated with an attenuation of the incident pressure fluctuations. When structural damping was taken into account significant noise attenuation compared to the undamped and the rigid cases was found. The noise reduction was attributed to reduced fluctuations of the structural deflections. Finally, the simulation of a fully turbulent flow convecting over an elastic TE showed qualitatively similar behaviour to the two dimensional studies. However, the excess noise from the structural motion was relatively more important than in the two dimensional case and the overall noise reduction was reduced. The motion of the elastic TE is shown to increase the energy in the low wave number spanwise modes.
Analytical modeling and experimental verification for vibration of piezoelectric U-shaped AFM incorporating thermal loading and surface effect

ABSTRACT In this work, an experimental model and a simplified analytical model of U-shaped atomic force microscopy (AFM) probe are described. The effects of surface elasticity, surface piezoelectricity and thermal loading on U-shaped probe are considered for the first time. The obtained experimental results are for commercial U-shaped probe AN2-300 when it is mounted on the afm+ system. The results indicate that modeled beams on the basis of the couple stress theory have higher stiffness than the modeled ones based on the classical continuum theory. It is shown that both the surface elasticity and surface residual stress influence the natural frequencies. Also, it can be observed that the piezoelectric field shows both decreasing and increasing effects depending on the sign of the voltage. In order to check the accuracy of the presented theory for the U-shaped AFM probe, the analytical results achieved by the modified couple stress theory are compared with experimental results, and it is found that they are in a very good agreement. This work will improve understanding the vibrational behavior of U-shaped probes and may be of special interest in design and application of nanoscale structures.