Miracle-dz/UniFAR-FTUs huggingface dataset:
Laurate Biosensors Image Brain Neurotransmitters In Vivo: Can an Antihypertensive Medication Alter Psychostimulant Behavior?

Neuromolecular Imaging (NMI) with novel biosensors enables the selective detection of neurotransmitters in vivo within seconds, on line and in real time. Biosensors remain in place for continuing studies over a period of months. This biotechnological advance is based on conventional electrochemistry; the biosensors detect neurotransmitters by electron transfer. Simply stated, biosensors adsorb electrons from each neurotransmitter at specific oxidation potentials; the current derived from electron transfer is proportional to neurotransmitter concentration. Selective electron transfer properties of these biosensors permit the imaging of neurotransmitters, metabolites and precursors. The novel BRODERICK PROBE® biosensors we have developed, differ in formulation and detection capabilities from biosensors/electrodes used in conventional electrochemistry/voltammetry. In these studies, NMI, specifically, the BRODERICK PROBE® laurate biosensor images neurotransmitter signals within mesolimbic neuronal terminals, nucleus accumbens (NAc); dopamine (DA), serotonin (5-HT), homovanillic acid (HVA) and L-tryptophan (L-TP) are selectively imaged. Simultaneously, we use infrared photobeams to monitor open-field movement behaviors on line with NMI in the same animal subjects. The goals are to investigate integrated neurochemical and behavioral effects of cocaine and caffeine alone and co-administered and further, to use ketanserin to decipher receptor profiles for these psychostimulants, alone and co-administered. The rationale for selecting this medication is: ketanserin (a) is an antihypertensive and cocaine and caffeine produce hypertension and (b) acts at 5-HT2A/2C receptors, prevalent in NAc and implicated in hypertension and cocaine addiction. Key findings are: (a) the moderate dose of caffeine simultaneously potentiates cocaine's neurochemical and behavioral responses. (b) ketanserin simultaneously inhibits cocaine-increased DA and 5-HT release in NAc and open-field behaviors and (c) ketanserin inhibits 5-HT release in NAc and open-field behaviors produced by caffeine, but, surprisingly, acts to increase DA release in NAc. Importantly, the latter effect may be a possible adverse effect of the moderate dose of caffeine in hypertensive patients. Thus, an antihypertensive medication is shown here to play a role in inhibiting brain reward possibly via antihypertensive mechanisms at DA and 5-HT receptor subtypes within DA motor neurons. An explanatory note for the results obtained, is the role likely played by the G Protein Receptor Complex (GPRC) family of proteins. Empirical evidence shows that GPRC dimers, heteromers and heterotrimers may cause cross-talk between distinct signalling cascade pathways in the actions of cocaine and caffeine. Ligand-directed functional selectivity, particularly for ketanserin, in addition to GPRCs, may also cause differential responses. The results promise new therapeutic strategies for drug addiction, brain reward and cardiovascular medicine.
Concentrations of serum constituents in cold-stressed calves from heifers fed inadequate protein and(or) energy.

A study with neonatal calves was conducted to determine the effects of maternal crude protein (CP) and(or) metabolizable energy (ME) malnutrition, cold stress (0 or 21 degrees C), and age on concentrations of selected serum constituents. For each of 2 yr, 60 artificially bred Angus heifers were assigned randomly to a 2 x 2 factorial nutritional plan 150 d before predicted parturition. The diets provided each heifer with either . 32 or . 96 kg/d of CP and 8.7 or 12.6 Mcal/d of ME. Blood samples were obtained from heifers at parturition and from their calves at birth and at 12, 24, 36, 48, and 72 h of age. Sera were analyzed for concentrations of blood urea nitrogen (BUN), creatinine (Creat), iron, total protein (TProt), alkaline phosphatase (AlkPhos), total bilirubin (TBil), and cholesterol (Chol). Mean correlations of these constituents in calf sera between 12-h adjacency intervals were high, but those between longer times (48 or 60 h) were low. Simple correlations of serum constituents between cows and calves at birth were low except for BUN (r = .578 and .295 for yr 1 and 2, respectively). There were significant main treatment effects for maternal CP consumption on BUN levels, for environmental temperature on BUN, Creat, and TBil levels, and for years on BUN, Creat, iron, and AlkPhos levels in calves. Significant polynomial relationships were found over hours of age for all variables. Blood urea N decreased in normal calves but remained relatively constant at a low level in deficient calves. Year x hour of age interactions occurred for iron, TProt, AlkPhos, TBil, and Chol. Protein x year x hour of age interactions were found for iron and Chol. These results suggest that random sampling times are not useful for decision making during the first 72 h after birth. Consideration must be given to multiple samples taken at specific calf ages, to environmental temperatures, and to maternal protein nutritional levels when interpreting calf blood sera data.
Telehealth Interventions for Management of Chronic Obstructive Lung Disease (COPD) and Asthma: A Critical Review

The authors systematically collated, classified, and evaluated the evidence of intervention studies from recent systematic reviews about the effects of telehealth interventions on COPD and asthma care. Eight electronic databases were searched. Eligible articles were those published between 2001 and 2011 in English. Eleven review articles are included. Asthma and COPD are better controlled when patients use interactive technological tools to monitor their chronic disease. The effects of telehealth interventions on emergency department attendance, specific quality of life, and mortality remained less certain. Only some reviews mentioned if the cost-effectiveness was systematically analyzed. Telehealth promises to be a highly effective intervention in managing chronic lung diseases while also potentially reducing some of the economic burdens of asthma and COPD. New directions in telehealth developments, implementations, and evaluations should be made, in which the exchange of health information should not be over simplified, but rather reflect the different socio-cultural practices of population groups and individuals.
WoSIT: A Word Sense Induction Toolkit for Search Result Clustering and Diversification

In this demonstration we present WoSIT, an API for Word Sense Induction (WSI) algorithms. The toolkit provides implementations of existing graph-based WSI algorithms, but can also be extended with new algorithms. The main mission of WoSIT is to provide a framework for the extrinsic evaluation of WSI algorithms, also within end-user applications such as Web search result clustering and diversification.
An historical cohort study of cardiac catheterization during childhood and the risk of cancer.

This study aimed to determine whether cancer risk was elevated among patients exposed to radiation from diagnostic cardiac catheterization during childhood. The study cohort included 3915 children who underwent at least one cardiac catheterization at a major children's hospital in Toronto, Canada, between 1950 and 1965, were < or = 18 years at the time of the procedure, and were residents of Ontario. Follow-up of the cohort was done by linkage to the Ontario Cancer Registry up to 1986. A total of seven cancer deaths were observed (O), compared with 5.7 expected (E) based on provincial cancer rates (mortality ratio [O/E] = 1.2; 90% confidence interval [CI] : 0.6-2.3). In the analysis of cancer incidence, 13 cancers were detected, compared with 17.3 expected (O/E = 0.75; 90% CI : 0.4-1.2). These mortality and incidence ratios were not statistically significantly elevated. Detailed analyses were conducted according to age at exposure, age at diagnosis, sex, number of procedures, year of first catheterization, and latent period. Detected deviations in risk were confined to an early period of exposure, indicating that later practices of cardiac catheterization were not associated with increased risk. In general, there was no statistically significant excess risk of cancer among the cohort.
A Price- and-Time-Slot-Negotiation Mechanism for Cloud Service Reservations

When making reservations for Cloud services, consumers and providers need to establish service-level agreements through negotiation. Whereas it is essential for both a consumer and a provider to reach an agreement on the price of a service and when to use the service, to date, there is little or no negotiation support for both price and time-slot negotiations (PTNs) for Cloud service reservations. This paper presents a multi-issue negotiation mechanism to facilitate the following: 1) PTNs between Cloud agents and 2) tradeoff between price and time-slot utilities. Unlike many existing negotiation mechanisms in which a negotiation agent can only make one proposal at a time, agents in this work are designed to concurrently make multiple proposals in a negotiation round that generate the same aggregated utility, differing only in terms of individual price and time-slot utilities. Another novelty of this work is formulating a novel time-slot utility function that characterizes preferences for different time slots. These ideas are implemented in an agent-based Cloud testbed. Using the testbed, experiments were carried out to compare this work with related approaches. Empirical results show that PTN agents reach faster agreements and achieve higher utilities than other related approaches. A case study was carried out to demonstrate the application of the PTN mechanism for pricing Cloud resources.
A Fast and Bumpy Ride

Abstract Whether one looks at the overall college career, the course of a single semester, or the vicissitudes of day-to-day college life, college students experience an extraordinary amount of change. While change promotes growth, it can also trigger psychological problems. Some common psychological consequences are overreactions to new stressors, disruption of eating, sleeping and studying routines, and identity-related concerns. Change also both helps and hinders the process of therapy. As experts in working with fluid situations, college therapists can help students understand and cope with the unrelenting changes in their lives.
Characterizing Land–Atmosphere Coupling and the Implications for Subsurface Thermodynamics

Abstract The objective of this work is to develop a Simple Land-Interface Model (SLIM) that captures the seasonal and interannual behavior of land–atmosphere coupling, as well as the subsequent subsurface temperature evolution. The model employs the one-dimensional thermal diffusion equation driven by a surface flux boundary condition. While the underlying physics is straightforward, the SLIM framework allows a qualitative understanding of the first-order controls that govern the seasonal coupling between the land and atmosphere by implicitly representing the dominant processes at the land surface. The model is used to perform a suite of experiments that demonstrate how changes in surface air temperature and coupling conditions control subsurface temperature evolution. The work presented here suggests that a collective approach employing both complex and simple models, when joined with analyses of observational data, has the potential to increase understanding of land–atmosphere coupling and the subsequen.
Role of Cytochrome P450 2B6 Pharmacogenomics in Determining Efavirenz‐Mediated Central Nervous System Toxicity, Treatment Outcomes, and Dosage Adjustments in Patients with Human Immunodeficiency Virus Infection

For treatment‐naive patients with human immunodeficiency virus infection, efavirenz (EFV), together with tenofovir and emtricitabine, was once widely prescribed given its efficacy and ease of administration in a combination pill. However, the high rate of central nervous system (CNS) toxicities from EFV prompted the U.S. Department of Health and Human Services to move the EFV‐based regimen from the recommended to the alternative category. For patients who do meet the criteria for newer recommended antiretroviral treatments, EFV is a viable option and often the mainstay of treatment outside the United States because newer antiretroviral treatments are more expensive. CNS toxicity occurring with the recommended standard dose of EFV remains a challenge and may in part be attributable to polymorphisms in cytochrome P450 (CYP) 2B6, the enzyme involved in the major metabolic pathway for converting EFV to inactive metabolites. Functionally deficient alleles of CYP2B6 such as CYP2B6*6, *18, and *22 may be responsible for significantly higher therapeutic concentrations of EFV at a standard dose of 600 mg/day. We conducted a thorough review of the reported studies to elucidate the relationship between polymorphisms in CYP2B6 with adverse events and treatment response, including virologic suppression, immunologic response, resistance, and discontinuation of treatment. Compelling evidence exists to support the case for CYP2B6 genotype–guided EFV therapy while acknowledging the need for prospective controlled clinical trials to evaluate its clinical utility.
Fetal bovine bone cells synthesize bone-specific matrix proteins

We isolated cells from both calvaria and the outer cortices of long bones from 3- to 5-mo bovine fetuses. The cells were identified as functional osteoblasts by indirect immunofluorescence using antibodies against three bone-specific, noncollagenous matrix proteins (osteonectin, the bone proteoglycan, and the bone sialoprotein) and against type 1 collagen. In separate experiments, confluent cultures of the cells were radiolabeled and shown to synthesize and secrete osteonectin, the bone proteoglycan and the bone sialoprotein by immunoprecipitation and fluorography of SDS polyacrylamide gels. Analysis of the radiolabeled collagens synthesized by the cultures showed that they produced predominantly (approximately 94%) type I collagen, with small amounts of types III and V collagens. In agreement with previous investigators who have employed the rodent bone cell system, we confirmed in bovine bone cells that (a) there was a typical cyclic AMP response to parathyroid hormone, (b) freshly isolated cells possessed high levels of alkaline phosphatase, which diminished during culture but returned to normal levels in mineralizing cultures, and (c) cells grown in the presence of ascorbic acid and beta-glycerophosphate rapidly produced and mineralized an extracellular matrix containing largely type I collagen. These results show that antibodies directed against bone-specific, noncollagenous proteins can be used to clearly identify bone cells in vitro.
Cytogenetic analysis in Chinese hamster cells chronically exposed to low doses of X‐rays

Purpose: It is important to develop simple experimental models to assess the induction of DNA damage and study the different factors involved under controlled conditions. This paper describes the cytogenetic analysis carried out in Chinese hamster cells (CHO) sequentially exposed to very low doses of X‐rays. Materials and methods: CHO cells were cultured for 14 passages. Irradiation treatment was performed once per passage, and three irradiation doses were employed: 2.5, 5.0 and 10.0 mSv. Results: Sequential irradiation of CHO cells did not increase the yield of chomatid‐ or chromosome‐type aberrations. However, a significant increase of achromatic lesions (gaps) was found after the first or second X‐ray dose, with all three irradiation doses employed. Conclusions: The variation in the frequency of gaps as well as that in the mitotic index during the 14 cycles of radiation could be an indication of the induction of genomic instability. According to this, continuous rises and falls in the frequency of gaps as well as in the mitotic index reflects the simultaneous induction of endogenous DNA damage, cell death and cell survival.
Biosynthetic Machinery Involved in Aberrant Glycosylation: Promising Targets for Developing of Drugs Against Cancer

Cancer cells depend on altered metabolism and nutrient uptake to generate and keep the malignant phenotype. The hexosamine biosynthetic pathway is a branch of glucose metabolism that produces UDP-GlcNAc and its derivatives, UDP-GalNAc and CMP-Neu5Ac and donor substrates used in the production of glycoproteins and glycolipids. Growing evidence demonstrates that alteration of the pool of activated substrates might lead to different glycosylation and cell signaling. It is already well established that aberrant glycosylation can modulate tumor growth and malignant transformation in different cancer types. Therefore, biosynthetic machinery involved in the assembly of aberrant glycans are becoming prominent targets for anti-tumor drugs. This review describes three classes of glycosylation, O-GlcNAcylation, N-linked, and mucin type O-linked glycosylation, involved in tumor progression, their biosynthesis and highlights the available inhibitors as potential anti-tumor drugs.
The role of agricultural use of insecticides in resistance to pyrethroids in Anopheles gambiae s.l. in Burkina Faso.

Agricultural use of insecticides is involved in the selection of resistance to these compounds in field populations of mosquitoes in Burkina Faso. Anopheles gambiae s.l. was resistant to permethrin and DDT in cotton-growing and urban areas, but susceptible in areas with limited insecticide selection pressure (rice fields and control areas). Nevertheless, resistance to these insecticides was observed in a village on the outskirts of the rice fields at the end of the rainy season, suggesting that the latter population of mosquitoes had migrated from the surrounding cotton villages into the rice fields. A seasonal variation of resistance observed in the cotton-growing area is related to the distribution of the molecular M and S forms of An. gambiae, since resistance to pyrethroids has so far only been reported in the S form. Pyrethroid resistance in west African An. gambiae was conferred by target site insensitivity through a knockdown resistance (kdr)-like mutation, which was present at high frequencies in mosquitoes in the cotton-growing and urban areas.
A generalized foundry CMOS platform for capacitively-transduced resonators monolithically integrated with amplifiers

A generalized foundry CMOS-MEMS platform suited for integrated micromechanical resonator circuits have been developed for commercial multi-user purpose and demonstrated with a fast turnaround time and a variety of design flexibilities for resonator applications. With this platform, different configurations of capacitively-transduced resonators monolithically integrated with their associated amplifier circuits, spanning frequencies from 500kHz to 14.5MHz, have been realized with resonator Q's around 2,000. This platform specifically featured with various configurations of structural materials, different arrangements of mechanical boundary conditions, large transduction area, well-defined anchors, and performance enhancement scaling with IC fabrication technology, offers a variety of flexible design options suited for sensor and RF applications.
Security scheduling for real-world networks

Network based security games, where a defender strategically places security measures on the edges of a graph to protect against an adversary, who chooses a path through a graph is an important research problem with potential for real-world impact. For example, police forces face the problem of placing checkpoints on roads to inspect vehicular traffic in their day-to-day operations, a security measure the Mumbai police have performed since the terrorist attacks in 2008. Algorithms for solving such network-based security problems have been proposed in the literature, but none of them scale up to solving problems of the size of real-world networks. In this paper, we present Snares, a novel algorithm that computes optimal solutions for both the defender and the attacker in such network security problems. Based on a double-oracle framework, Snares makes novel use of two approaches: warm starts and greedy responses. It makes the following contributions: (1) It defines and uses mincut-fanout, a novel method for efficient warm-starting of the computation; (2) It exploits the sub-modularity property of the defender optimization in a greedy heuristic, which is used to generate "better-responses"; Snares} also uses a better-response computation for the attacker. Furthermore, we evaluate the performance of Snares in real-world networks illustrating a significant advance: whereas state-of-the-art algorithms could handle just the southern tip of Mumbai, {\sc Snares} can compute optimal strategy for the entire urban road network of Mumbai.
Change-Point Detection in a Sequence of Bags-of-Data

In this paper, the limitation that is prominent in most existing works of change-point detection methods is addressed by proposing a nonparametric, computationally efficient method. The limitation is that most works assume that each data point observed at each time step is a single multi-dimensional vector. However, there are many situations where this does not hold. Therefore, a setting where each observation is a collection of random variables, which we call a bag of data, is considered. After estimating the underlying distribution behind each bag of data and embedding those distributions in a metric space, the change-point score is derived by evaluating how the sequence of distributions is fluctuating in the metric space using a distance-based information estimator. Also, a procedure that adaptively determines when to raise alerts is incorporated by calculating the confidence interval of the change-point score at each time step. This avoids raising false alarms in highly noisy situations and enables detecting changes of various magnitudes. A number of experimental studies and numerical examples are provided to demonstrate the generality and the effectiveness of our approach with both synthetic and real datasets.
EFECTO DE LA MAGNITUD DEL ESTÍMULO SOBRE LA CONFIANZA EN LOS JUICIOS PSICOFÍSICOS

Effect of the stimulus magnitude on the confidence of psychophysical judgments. Eventhough quantitative judgments are clearly affected by non-sensory factors, the principal component of the response variance has always being sensory magnitudes (Garriga-Trillo, 1992; Baird, in press). Olsson & Winman (1996) have found that confidence judgments on detection responses are affected by stimulus characteristics. Confidence judgments of quantitative responses have not been studied on this issue. This work studies if the stimulus magnitude influences confidence judgments of quantitative psychophysical responses considering a line length estimation and a time duration task. Independent of the sensory modality involved, mainly, there are no significant differences between the confidence distributions for each stimulus. Metajudgments of confidence in estimation tasks are not affected by stimulus magnitude. This could favor, within the two-process theory, the cognitive nature of confidence judgments.