Miracle-dz/UniFAR-FTUs huggingface dataset:
Wholegrain fermentation affects gut microbiota composition, phenolic acid metabolism and pancreatic beta cell function in a rodent model of type 2 diabetes

The intestinal microbiota plays an important role in host metabolism via production of dietary metabolites. Microbiota imbalances are linked to type 2 diabetes (T2D), but dietary modification of the microbiota may promote glycemic control. Using a rodent model of T2D and an in vitro gut model system, this study investigated whether differences in gut microbiota between control mice and mice fed a high-fat, high-fructose (HFHFr) diet influenced the production of phenolic acid metabolites following fermentation of wholegrain (WW) and control wheat (CW). In addition, the study assessed whether changes in metabolite profiles affected pancreatic beta cell function. Fecal samples from control or HFHFr-fed mice were fermented in vitro with 0.1% (w/v) WW or CW for 0, 6, and 24 h. Microbiota composition was determined by bacterial 16S rRNA sequencing and phenolic acid (PA) profiles by UPLC-MS/MS. Cell viability, apoptosis and insulin release from pancreatic MIN6 beta cells and primary mouse islets were assessed in response to fermentation supernatants and selected PAs. HFHFr mice exhibited an overall dysbiotic microbiota with an increase in abundance of proteobacterial taxa (particularly Oxalobacteraceae) and Lachnospiraceae, and a decrease in Lactobacillus. A trend toward restoration of diversity and compositional reorganization was observed following WW fermentation at 6 h, although after 24 h, the HFHFr microbiota was monodominated by Cupriavidus. In parallel, the PA profile was significantly altered in the HFHFr group compared to controls with decreased levels of 3-OH-benzoic acid, 4-OH-benzoic acid, isoferulic acid and ferulic acid at 6 h of WW fermentation. In pancreatic beta cells, exposure to pre-fermentation supernatants led to inhibition of insulin release, which was reversed over fermentation time. We conclude that HFHFr mice as a model of T2D are characterized by a dysbiotic microbiota, which is modulated by the in vitro fermentation of WW. The differences in microbiota composition have implications for PA profile dynamics and for the secretory capacity of pancreatic beta cells.
Translating controlled release systems from biomedicine to agriculture

Sustainable food production is a grand challenge facing the global economy. Traditional agricultural practice requires numerous interventions, such as application of nutrients and pesticides, of which only a fraction are utilized by the target crop plants. Controlled release systems (CRSs) designed for agriculture could improve targeting of agrochemicals, reducing costs and improving environmental sustainability. CRSs have been extensively used in biomedical applications to generate spatiotemporal release patterns of targeted compounds. Such systems protect encapsulant molecules from the external environment and off-target uptake, increasing their biodistribution and pharmacokinetic profiles. Advanced ‘smart’ release designs enable on-demand release in response to environmental cues, and theranostic systems combine sensing and release for real-time monitoring of therapeutic interventions. This review examines the history of biomedical CRSs, highlighting opportunities to translate biomedical designs to agricultural applications. Common encapsulants and targets of agricultural CRSs are discussed, as well as additional demands of these systems, such as need for high volume, low cost, environmentally friendly materials and manufacturing processes. Existing agricultural CRSs are reviewed, and opportunities in emerging systems, such as nanoparticle, ‘smart’ release, and theranostic formulations are highlighted. This review is designed to provide a guide to researchers in the biomedical controlled release field for translating their knowledge to agricultural applications, and to provide a brief introduction of biomedical CRSs to experts in soil ecology, microbiology, horticulture, and crop sciences.
The effects of rhein on D-GalN/LPS-induced acute liver injury in mice: Results from gut microbiome-metabolomics and host transcriptome analysis

Background Rhubarb is an important traditional Chinese medicine, and rhein is one of its most important active ingredients. Studies have found that rhein can improve ulcerative colitis by regulating gut microbes, but there are few reports on its effects on liver diseases. Therefore, this study aims to investigate these effects and underlying mechanisms. Methods Mice were given rhein (100 mg/kg), with both a normal control group and a model group receiving the same amount of normal saline for one week. Acute liver injury was induced in mice by intraperitoneal injection of D-GalN (800 mg/kg)/LPS (10 ug/kg). Samples (blood, liver, and stool) were then collected and assessed for histological lesions and used for 16S rRNA gene sequencing, high-performance liquid chromatography-mass spectrometry (LC-MS) and RNA-seq analysis. Results The levels of ALT and AST in the Model group were abnormal higher compared to the normal control group, and the levels of ALT and AST were significantly relieved in the rhein group. Hepatic HE staining showed that the degree of liver injury in the rhein group was lighter than that in the model group, and microbiological results showed that norank_o:Clostridia_UCG-014, Lachnoclostridium, and Roseburia were more abundant in the model group compared to the normal control group. Notably, the rhein treatment group showed reshaped disturbance of intestinal microbial community by D-GalN/LPS and these mice also had higher levels of Verrucomicrobia, Akkermansiaceae and Bacteroidetes. Additionally, There were multiple metabolites that were significantly different between the normal control group and the model group, such as L-α-amino acid, ofloxacin-N-oxide, 1-hydroxy-1,3-diphenylpropan-2-one,and L-4-hydroxyglutamate semialdehyde, but that returned to normal levels after rhein treatment. The gene expression level in the model group also changed significantly, various genes such as Cxcl2, S100a9, Tnf, Ereg, and IL-10 were up-regulated, while Mfsd2a and Bhlhe41 were down-regulated, which were recovered after rhein treatment. Conclusion Overall, our results show that rhein alleviated D-GalN/LPS-induced acute liver injury in mice. It may help modulate gut microbiota in mice, thereby changing metabolism in the intestine. Meanwhile, rhein also may help regulate genes expression level to alleviate D-GalN/LPS-induced acute liver injury.
Clinical assessment of resting full-cycle ratio and fractional flow reserve for coronary artery disease in a real-world cohort

Background There are few reports published on the comparison of the resting full-cycle ratio (RFR) and fractional flow reserve (FFR) on the assessment of the severity of coronary stenosis. We aimed to investigate the diagnostic accuracy of RFR for detection of functionally significant coronary lesions. Methods This was an observational, retrospective, single-center study. We evaluated both RFR and FFR for 277 coronary lesions of 235 patients who underwent coronary angiography. Patients presenting with chronic coronary syndrome, unstable angina, or non-ST-elevation myocardial infarction were included. Results The mean FFR and RFR values were 0.84 ± 0.08 and 0.90 ± 0.08, respectively. RFR significantly correlated with FFR (r = 0.727, P < 0.001). The agreement rate between the FFR and RFR was 79.8% (221/277). The diagnostic performance of RFR vs. FFR was accuracy 79.8%, sensitivity 70.4%, specificity 83.7%, positive predictive value 64.0%, and negative predictive value 87.2%. The discriminative power of RFR to identify lesions with FFR ≤ 0.80 was acceptable when the RFR value was within the gray zone [0.86 ≤ RFR ≤ 0.93; AUC: 0.72 (95% CI:0.63–0.81)], while it was excellent when the RFR value was out of the gray zone [RFR > 0.93 or < 0.86; AUC: 0.94 (95% CI:0.88–0.99)]. Conclusion RFR was significantly correlated with FFR in the assessment of intermediate coronary stenosis. An RFR-FFR hybrid approach increases the diagnostic accuracy of RFR in the detection of functionally significant lesions.
Leveraging the potential of synthetic text for AI in mental healthcare

In today’s world it seems fair to say that extensive digital data sharing is the price we pay for the technological advances we have seen achieved as a result of AI systems analysing large quantities of data in a relatively short time. Where such AI is used in the realm of mental health, this data sharing poses additional challenges not just due to the sensitive nature of the data itself but also the potential vulnerability of the data donors themselves should there be a cybersecurity data breach. To address the problem, the AI community proposes to use synthetic text preserving only the salient properties of the original. Such text has potential to fill gaps in the textual data availability (e.g., rare conditions or under-represented groups) while reducing exposure. Our perspective piece is aimed to demystify the process of generating synthetic text, explain its algorithmic and ethical challenges, especially for the mental health domain, as well as most promising ways of overcoming them. We aim to promote better understanding and as a result acceptability of synthetic text outside the research community.
Sobolev, Besov and Triebel-Lizorkin spaces on quantum tori

This paper gives a systematic study of Sobolev, Besov and Triebel-Lizorkin spaces on a noncommutative $d$-torus $\mathbb{T}^d_\theta$ (with $\theta$ a skew symmetric real $d\times d$-matrix). These spaces share many properties with their classical counterparts. We prove, among other basic properties, the lifting theorem for all these spaces and a Poincar\'e type inequality for Sobolev spaces. We also show that the Sobolev space $W^k_\infty(\mathbb{T}^d_\theta)$ coincides with the Lipschitz space of order $k$, already studied by Weaver in the case $k=1$. We establish the embedding inequalities of all these spaces, including the Besov and Sobolev embedding theorems. We obtain Littlewood-Paley type characterizations for Besov and Triebel-Lizorkin spaces in a general way, as well as the concrete ones in terms of the Poisson, heat semigroups and differences. Some of them are new even in the commutative case, for instance, our Poisson semigroup characterizations improve the classical ones. As a consequence of the characterization of the Besov spaces by differences, we extend to the quantum setting the recent results of Bourgain-Br\'ezis -Mironescu and Maz'ya-Shaposhnikova on the limits of Besov norms. The same characterization implies that the Besov space $B^\alpha_{\infty,\infty}(\mathbb{T}^d_\theta)$ for $\alpha>0$ is the quantum analogue of the usual Zygmund class of order $\alpha$. We investigate the interpolation of all these spaces, in particular, determine explicitly the K-functional of the couple $(L_p(\mathbb{T}^d_\theta), \, W^k_p(\mathbb{T}^d_\theta))$, which is the quantum analogue of a classical result due to Johnen and Scherer. Finally, we show that the completely bounded Fourier multipliers on all these spaces do not depend on the matrix $\theta$, so coincide with those on the corresponding spaces on the usual $d$-torus.
Coordinate systems for the space shuttle program

A minimal set of well defined coordinate systems necessary for the interchange of data within the space shuttle program is presented. The document format consists of four parts: (1) a list of the subscripts identifying the coordinate systems, (2) a glossary explaning the terms used within the coordinate system definitions, (3) figures defining, both graphically and verbally, each coordinate system, and (4) an appendix (published separately) showing the relationships (transformations) between similar systems.
The critical BMI hypothesis for puberty initiation and the gender prevalence difference: Evidence from an epidemiological survey in Beijing, China

Background Although previous studies suggested that there is a certain level of body fat mass before puberty can be initiated, most studies have focused on girls. Objective To investigate the relationship between precocious puberty and physical growth in school-aged children in Beijing, China. Methods 7590 Chinese children (3591 girls and 3999 boys) aged 6–11 years were recruited in Beijing, China. Body mass index (BMI) categories were defined by WHO Child Growth Standards and central obesity were defined by sex-specific waist-to-height ratio cut-offs (≥0.46 for girls, ≥0.48 for boys). Sexual development was assessed using Tanner criteria. Results The prevalence of general obesity and central obesity among boys was higher than that in girls. Girls had a significantly higher precocious puberty rate than boys (5.93% vs. 0.87%), particularly in those aged 7 years old (9.20%). Children in the general obesity and central obesity groups have a higher prevalence of precocious puberty and earlier median ages for the attainment of Tanner B2/T2. For girls with Tanner stages≥II at 6-year-old and 7-year-old, the mean BMI was equivalent to the 50th centile of a normal 9.9-year-old and 11.9-year-old girl, respectively. The mean BMI of boys with Tanner stages≥II at 7-year-old and 8-year-old was correspondent to the 50th centile of a normal 14-year-old and 15.3-year-old boy, respectively. For girls, general obesity appears to contribute to the risk of the development of precocious puberty to a greater extent than central obesity does. For boys, central obesity, but not general obesity, was an independent risk factor for precocious puberty. Conclusions The prevalence of childhood obesity and precocious puberty was high in China. Precocious puberty was correlated with a large BMI. Boys had a higher threshold of BMI for puberty development than girls. Children with precocious puberty, particularly those with central obesity, should be aware of adverse cardiovascular events.
Exploring the impact of perceived risk and trust on tourist acceptance intentions in the post-COVID-19 era: A case study of Hainan residents

Hainan, is the only free trade port that also exudes quintessence of the culture of China. Tourism is one of Hainan's most lucrative industries. On the one hand, the regional economy is flourishing and on the other hand, the economy is facing unprecedented impacts from the COVID-19 pandemic. In response to the affected global market environment, this study investigates Hainan residents' acceptance intentions, or tolerance, of tourists. Here, based on the theory of reasoned action, which includes “subjective norm” combined with “trust,” “perceived risk,” and “perceived value. ” Using “resident attitude” as a mediator, theoretical research frameworks were developed. A total of 447 valid responses were collected using online and paper-copy questionnaires distributed to Hainan residents from 15 July 2021 to 10 November 2021. The data from the questionnaires were used for three analyses namely, descriptive statistical analysis, measurement model verification, and structural equation modeling analysis. Findings show a positive effect of trust on residents' attitudes in Hainan; perceived value and subjective norm showed a positive effect on resident acceptance intentions for tourism; the perceived risk of residents showed a more negative effect on attitudes toward tourists, but the influence was small. Finally, through the results of the study, theoretical and practical implications in a post-pandemic era are discussed.
Potential value of serum brain-derived neurotrophic factor, vascular endothelial growth factor, and S100B for identifying major depressive disorder in knee osteoarthritis patients

Background The chronic pain and functional limitations in osteoarthritis (OA) patients can increase risk of psychiatric disorders, e.g., major depression disorder (MDD), which may further aggravate the clinical symptoms of OA. Early detection of MDD is essential in the clinical practice of OA. Materials and methods Two hundred and fifteen participants with knee OA were recruited, including 134 MDD patients (i.e., MDD group) and 81 ones without MDD (i.e., control group). Among them, 81 OA participants in the control group received a 3-year follow-up and were divided into trans-MDD group (who transforming into MDD; N = 39) and non-MDD group (who keeping non-MDD; N = 42) at the end of the follow-up. The 17-item Hamilton Depression Scale (HAMD-17), Self-Rating Depression Scale (SDS), and Visual Analogue Scale (VAS) were performed. Furthermore, serum levels of brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), S100B, and IGF-1 were detected. Results (1) Compared with OA participants without MDD, there were significant decrease in serum BDNF and significant increase in serum VEGF and S100B and VAS scores in OA participants with MDD. (2) A mediation of the association was found between the VAS scores and the HAMD-17 scores through the BDNF as mediator in OA participants with MDD. (3) Significantly lower baseline BDNF levels and higher baseline S100B levels were detected in OA participants who transforming to MDD after a 3-year follow-up when compared with those who keeping non-MDD. (4) In the trans-MDD group, significant associations of the change of serum BDNF levels with rate of change of HAMD-17 scores were found, and baseline serum S100B levels positively correlated with the HAMD-17 scores at the end of the follow-up. (5) In OA participants, the composite indicator of BDNF, VEGF, and S100B differentiated MDD patients from controls with the area under the curve (AUC) value of 0.806, and the combined indicator of baseline BDNF and S100B distinguished trans-MDD participants from non-MDD ones with an AUC value of 0.806. Conclusion Serum BDNF, VEGF, and S100B may be potential biomarkers to identify MDD in OA patients. Meanwhile, serum BDNF and S100B shows great potential to predict the risk of MDD for OA.
Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc.

Flavonoids from Actinidia arguta Sieb. Zucc. can reduce uric acid in mice. However, the molecular basis of its biosynthesis is still unclear. In this paper, we used a combination of extensively targeted metabolomics and transcriptomics analysis to determine the types and differences of flavonoids in the fruit ripening period (August to September) of two main cultivated varieties in northern China. The ethanol extract was prepared, and the potential flavonoids of Chrysin (Flavone1), Rutin (Flavone2), and Daidzein (Flavone3) in Actinidia arguta Sieb. Zucc. were separated and purified by HPD600 macroporous adsorption resin and preparative liquid chromatography. The structure was identified by MS-HPLC, and the serum uric acid index of male Kunming mice was determined by an animal model test.125 flavonoids and 50 differentially regulated genes were identified. The contents of UA (uric acid), BUN (urea nitrogen), Cr (creatinine), and GAPDH in mouse serum and mouse liver glycogen decreased or increased in varying degrees. This paper reveals the biosynthetic pathway of uric acid-reducing flavonoids in the fruit of Actinidia arguta Sieb. Zucc. , a major cultivar in northern China, provides valuable information for the development of food and drug homologous functional foods.
Progress and challenges in adaptive robotics

Adaptive robotics achieved tremendous progress during the last few years (see Nolfi (2021) for an introduction and review). The term adaptive robotics refers to methods which permit the design of robots capable of developing their skills autonomously through an evolutionary and/or learning process. It focuses on approaches requiring minimal human intervention in which the behavior displayed by the robots and the control rules producing such behavior are discovered by an adaptive process automatically on the basis of a reward or fitness function which rates how well the robot is doing. It focuses on end-to-end learning, i.e. on systems which receive as input directly the state of robot’s sensors and determine directly the state of the robot’s actuators, without involving any type of hand-designed pre-processing. Finally, it focuses on model-free methods, i.e. on systems which do not have an internal model of the environment, or in which the internal model is acquired automatically during the adaptation process. In this article I will review the major advances and the research challenges.
Molecular characterization, targeting and expression analysis of chloroplast and mitochondrion protein import components in Nicotiana benthamiana

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex.