Miracle-dz/UniFAR-FTUs huggingface dataset:
Retention of African American women in cancer genetics research

Although retention is a critical component of longitudinal cancer genetics research, limited empirical data are available on predictors of study retention among populations that are difficult to enroll. We evaluated predictors of retention in cancer genetics research among African American women at increased risk for having a BRCA1 and BRCA2 (BRCA1/2) mutation. Participants were African American women (n = 192) at increased risk for hereditary breast‐ovarian cancer who were enrolled in a longitudinal genetic counseling research study. Retention was evaluated separately for the 1‐ and 6‐month follow‐ups and in terms of overall retention (e.g., completion of both telephone interviews). Seventy‐three percent of women and 65% of women were retained at the 1‐ and 6‐month follow‐ups respectively; in terms of overall retention, 60% of women were retained in both follow‐up telephone interviews. Predictors of retention at 1‐month included being employed (OR = 2.47, 95% CI = 1.24, 4.93, P = 0.01) whereas predictors of overall retention included having a personal history of breast and/or ovarian cancer (OR = 2.06, 95% CI = 1.07, 3.95, P = 0.03) and having completed genetic counseling (OR = 2.63, 95% CI = 1.39, 4.98, P = 0.003). These data suggest that once enrolled in genetic counseling research, the majority of African American women will continue to participate, especially if concrete clinical services are provided. © 2007 Wiley‐Liss, Inc.
The Drosophila Imd Signaling Pathway

The fruit fly, Drosophila melanogaster, has helped us to understand how innate immunity is activated. In addition to the Toll receptor and the Toll signaling pathway, the Drosophila immune response is regulated by another evolutionarily conserved signaling cascade, the immune deficiency (Imd) pathway, which activates NF-κB. In fact, the Imd pathway controls the expression of most of the antimicrobial peptides in Drosophila; thus, it is indispensable for normal immunity in flies. In this article, we review the current literature on the Drosophila Imd pathway, with special emphasis on its role in the (patho)physiology of different organs. We discuss the systemic response, as well as local responses, in the epithelial and mucosal surfaces and the nervous system.
Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid

Small non-coding RNAs of 18–25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3′ end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1.
IFN Regulatory Factor-4 and -8 Govern Dendritic Cell Subset Development and Their Functional Diversity

Dendritic cells (DCs) are bone marrow (BM)-derived APCs central to both innate and adaptive immunity. DCs are a heterogeneous cell population composed of multiple subsets with diverse functions. The mechanism governing the generation of multiple DC subsets is, however, poorly understood. In this study we investigated the roles of closely related transcription factors, IFN regulatory factor (IRF)-4 and IRF-8, in DC development by analyzing IRF-4−/−, IRF-8−/−, and IRF-4−/−IRF-8−/− (double-knockout) mice. We found that IRF-4 is required for the generation of CD4+ DCs, whereas IRF-8 is, as reported previously, essential for CD8α+ DCs. Both IRFs support the development of CD4−CD8α− DCs. IRF-8 and, to a lesser degree, IRF-4 contribute to plasmacytoid DC (PDC) development. Thus, the two IRFs together regulate the development of all conventional DCs as well as PDCs. Consistent with these findings, IRF-4, but not IRF-8, was expressed in CD4+ DCs, whereas only IRF-8 was expressed in CD8α+ DCs. CD4−CD8α− DCs and PDCs expressed both IRFs. We also demonstrate in vitro that GM-CSF-mediated DC differentiation depends on IRF-4, whereas Fms-like tyrosine kinase 3 ligand-mediated differentiation depends mainly on IRF-8. Gene transfer experiments with double-knockout BM cells showed that both IRFs have an overlapping activity and stimulate a common process of DC development. Nonetheless, each IRF also possesses a distinct activity to stimulate subset-specific gene expression, leading to the generation of functionally divergent DCs. Together, IRF-4 and IRF-8 serve as a backbone of the molecular program regulating DC subset development and their functional diversity.
Patterns of Insecticide Resistance in Onion Thrips (Thysanoptera: Thripidae) in Onion Fields in New York

Abstract To develop an insecticide resistance management program for onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), on onions (Allium spp.), we surveyed populations in commercial onion fields in New York and evaluated their susceptibility to the two most widely used classes of insecticides plus two new insecticides during 2003–2005. All insecticide evaluations were conducted using the Thrips Insecticide Bioassay System (TIBS). As in our surveys conducted during 2002–2003, there were large temporal and spatial variations in susceptibility to the pyrethroid &lgr;-cyhalothrin (Warrior) across onion-growing regions in 2003. New data indicate that the field rate of methomyl (Lannate LV) still provides control but that the genes for resistance to methomyl are present in some populations. Tests with the two new insecticides, acetamiprid (Assail 70 WP) and spinosad (SpinTor 2CS), indicated they provided >85% mortality at the field rate. To determine the spatial variation in insecticide susceptibility within a region, a series of systematic assays were conducted with &lgr;-cyhalothrin and methomyl. In 2004 and 2005, our data indicated that the within-region spatial variation in susceptibility to &lgr;-cyhalothrin was not large at the field rate or for the 100 ppm rate of methomyl. In 2005, a year in which T. tabaci densities in most fields were much higher than in 2004, growers were unable to control T. tabaci in particular fields and attributed this lack of control to resistance. Yet, we found similar levels of high susceptibility in all fields when using TIBS. This finding suggests that resistance had not developed and that variation in control may have been due to other factors, such as localized higher populations, poor spray coverage, too much time between spray applications, or different onion varieties.
Genotoxicity evaluation of locally produced nano-hydroxyapatite-silica: An in vitro study using the bacterial reverse mutation test

Background: Nanohydroxyapatite-silica (nanoHA-Silica) has been produced by one-pot sol-gel technique. The material when incorporated into commercial Glass Ionomer Cement (GIC) was found to exhibit higher Vickers hardness, compressive strength, and flexural strength compared to conventional GIC. However, before starting to be used and exposed to the human cell, every material product should undergo for genotoxic evaluation. Thus, the objective of this in vitro study was to evaluate the genotoxicity of locally produced nanoHA-Silica under bacterial reverse mutation assay (Ames test). Materials and Methods: Four Salmonella typhimurium strains TA98, TA102, TA1535, and TA1537 were incubated with nanoHA-Silica in the presence and absence of exogenous metabolic activation system (S9) at five different concentrations (0.3125, 0.625, 1.25, 2.5, and 5 mg/plate) along with appropriate positive and negative controls. The assessment of the results was based on the number of revertant colonies in each plate, and the results were regarded as mutagenic when the number of revertant colonies was more than two-fold of the negative control. Results: There was no significant increase in the number of revertant colonies corresponding to the increase in the concentrations of the test substance for all the five bacterial strains treated with or without S9. Conclusion: NanoHA-Silica-GIC was non-genotoxic and had no mutagenic potential under present test conditions.
Identification of a familial cluster of pulmonary Mycobacterium bovis disease.

SETTING Local public health department. DESIGN Retrospective review of a cluster of three pulmonary Mycobacterium bovis cases occurring in a family, with genotyping of M. bovis strains isolated from the family members. RESULTS The genotypes of the M. bovis isolates were identical, as determined by three different methods: IS6110 restriction fragment length polymorphism, spoligoytping and mycobacterial interspersed repetitive units-variable number tandem repeat analyses. CONCLUSION The identification of three acid-fast bacilli (AFB) smear-positive pulmonary M. bovis cases, presenting in a single family and caused by an identical strain, suggests that person-to-person transmission of this organism may have occurred, although infection of one or more family members through ingestion of a contaminated dairy product could not be excluded.
In Vitro Metabolism of Pyrethroid Pesticides by Rat and Human Hepatic Microsomes and Cytochrome P450 Isoforms

Species differences in the intrinsic clearance (CLint) and the enzymes involved in the metabolism of pyrethroid pesticides were examined in rat and human hepatic microsomes. The pyrethroids bifenthrin, S-bioallethrin, bioresmethrin, β-cyfluthrin, cypermethrin, cis-permethrin, and trans-permethrin were incubated in rat and human hepatic microsomes in the presence or absence of NADPH. Metabolism was measured using a parent depletion approach. The CLint of the pyrethroids was 5- to 15-fold greater in rat relative to human microsomes except for trans-permethrin, which was approximately 45% greater in human microsomes. The metabolism of bifenthrin, S-bioallethrin, and cis-permethrin in rat and human hepatic microsomes was solely the result of oxidative processes. The metabolism of bioresmethrin and cypermethrin in human hepatic microsomes was solely the result of hydrolytic processes. Bioresmethrin and cypermethrin in rat hepatic microsomes and β-cyfluthrin and trans-permethrin in microsomes from both species were metabolized by both oxidative and hydrolytic pathways. The metabolism of trans-permethrin was reduced when incubated with its diastereomer, cis-permethrin, in both rat and human hepatic microsomes. Rat cytochrome P450 (P450) isoforms that showed activity toward several pyrethroids included CYP1A1, CYP1A2, CYP2C6, CYP2C11, CYP3A1, and CYP3A2. Human P450 isoforms that showed activity toward multiple pyrethroids were CYP2C8, CYP2C9, CYP2C19, and CYP3A4. Species-specific differences in metabolism may result in variable detoxification of pyrethroids, which may in turn result in divergent neurotoxic outcomes. These species differences and isomer interactions in metabolism of pyrethroids should be considered when assessing the potential adverse health effects of pyrethroid pesticides.
Cost-Effectiveness Analysis of Risk-Factor Guided and Birth-Cohort Screening for Chronic Hepatitis C Infection in the United States

Background No consensus exists on screening to detect the estimated 2 million Americans unaware of their chronic hepatitis C infections. Advisory groups differ, recommending birth-cohort screening for baby boomers, screening only high-risk individuals, or no screening. We assessed one-time risk assessment and screening to identify previously undiagnosed 40–74 year-olds given newly available hepatitis C treatments. Methods and Findings A Markov model evaluated alternative risk-factor guided and birth-cohort screening and treatment strategies. Risk factors included drug use history, blood transfusion before 1992, and multiple sexual partners. Analyses of the National Health and Nutrition Examination Survey provided sex-, race-, age-, and risk-factor-specific hepatitis C prevalence and mortality rates. Nine strategies combined screening (no screening, risk-factor guided screening, or birth-cohort screening) and treatment (standard therapy–peginterferon alfa and ribavirin, Interleukin-28B-guided (IL28B) triple-therapy–standard therapy plus a protease inhibitor, or universal triple therapy). Response-guided treatment depended on HCV genotype. Outcomes include discounted lifetime costs (2010 dollars) and quality adjusted life-years (QALYs). Compared to no screening, risk-factor guided and birth-cohort screening for 50 year-olds gained 0.7 to 3.5 quality adjusted life-days and cost $168 to $568 per person. Birth-cohort screening provided more benefit per dollar than risk-factor guided screening and cost $65,749 per QALY if followed by universal triple therapy compared to screening followed by IL28B-guided triple therapy. If only 10% of screen-detected, eligible patients initiate treatment at each opportunity, birth-cohort screening with universal triple therapy costs $241,100 per QALY. Assuming treatment with triple therapy, screening all individuals aged 40–64 years costs less than $100,000 per QALY. Conclusions The cost-effectiveness of one-time birth-cohort hepatitis C screening for 40–64 year olds is comparable to other screening programs, provided that the healthcare system has sufficient capacity to deliver prompt treatment and appropriate follow-on care to many newly screen-detected individuals.
La financiacin sanitaria autonmica: un problema sin resolver

A data processing system (20) has a cache (26) that does not use a TAG array for storing a TAG address as in a conventional cache. The cache (26), according to one embodiment, uses a state machine (30) for transitioning the cache (26) to an active state in response to a change of flow instruction which is a short backward branch instruction of a predetermined displacement. The predetermined displacement is less than the number of entries in the cache (26), so the cache can remain active as long as the program is in a loop which can be contained entirely within the cache. A look ahead feature for the valid bit array is provided that associates the valid bit for a current instruction with a previous instruction, such that during a read of the cache, the valid bit for a next instruction is checked with the same index used to read the current instruction.
Improving computer architecture simulation methodology by adding statistical rigor

Due to cost, time, and flexibility constraints, computer architects use simulators to explore the design space when developing new processors and to evaluate the performance of potential enhancements. However, despite this dependence on simulators, statistically rigorous simulation methodologies are typically not used in computer architecture research. A formal methodology can provide a sound basis for drawing conclusions gathered from simulation results by adding statistical rigor and, consequently, can increase the architect's confidence in the simulation results. This paper demonstrates the application of a rigorous statistical technique to the setup and analysis phases of the simulation process. Specifically, we apply a Plackett and Burman design to: 1) identify key processor parameters, 2) classify benchmarks based on how they affect the processor, and 3) analyze the effect of processor enhancements. Our results showed that, out of the 41 user-configurable parameters in SimpleScalar, only 10 had a significant effect on the execution time. Of those 10, the number of reorder buffer entries and the L2 cache latency were the two most significant ones, by far. Our results also showed that instruction precomputation - a value reuse-like microarchitectural technique - primarily improves the processor's performance by relieving integer ALU contention.
Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines

Oral administration of DSS has been reported to induce an acute and chronic colitis in mice. The aim of our study was to evaluate if the chronic phase of DSS‐induced colitis was characterized by a Th1/Th2 response and how this would relate to mucosal regeneration. Swiss Webster mice were fed 5% DSS in their drinking water for 7 days, followed by 2–5 weeks consumption of water. Control mice received only water. The animals were killed at 3 and 6 weeks after induction. Their colons were isolated for histology and immunohistochemistry, using specific MoAbs for T and B cells, macrophages, interferon‐gamma (IFN‐γ), IL‐4 and IL‐5. Colons were scored for inflammation, damage and regeneration. Two weeks after stopping DSS the colonic epithelium had only partially healed. Total colitis scores were still increased, especially in the distal colon, which was due to more inflammation, damage and less regeneration. In areas of incomplete colonic healing the basal parts of the lamina propria contained macrophages and CD4+ T cells. These CD4+ T cells showed a focal increase of IFN‐γ and IL‐4 staining compared with control animals. These findings were still observed 5 weeks after stopping DSS in some mice, albeit less extensive. Chronic DSS‐induced colitis is characterized by focal epithelial regeneration and a Th1 as well as Th2 cytokine profile. We postulate that chronic immune activation mediated by both populations of Th cells can interfere with colonic healing and can play a role in the pathogenesis of chronic colitis.
Production of Mayonnaise from Date Pit Oil

Chemical compositionof the residual pits from processing of date (Phoenix dactylifera L.) variety khalas from AL-Hasa region–Saudi Arabia was investigated. The extracted oil from residual pits was analyzed for its physico-chemical properties, (refractive index, colour, acid value, peroxide value, iodine number, saponification number and unsaponifiable matter), total phenols, tocopherol content, fatty acid composition, hydrocarbons, and sterol compounds by gas liquid chromatography, and oxidative stability by Rancimat method at 100℃ ± 2℃. Mineral content of date pits was analyzed by atomic absorption spectrophotometry. Date pit oil was used to replace conventional oil in producing mayonnaise, and sensory qualities were evaluated in comparison with commercial mayonnaise prepared from corn oil. The data demonstrated that mayonnaise containing date pit oil was superior in sensory characteristics as compared with control manufactured from corn oil. Results showed that the date pit oil could be used as nontraditional oil in some food processing such as mayonnaise products.
Importance Sampling for Production Rendering

Importance sampling provides a practical, production-proven method for integrating diffuse and glossy surface reflections with arbitrary image-based environment or area lighting constructs. Here, functions are evaluated at random points across a domain to produce an estimate of an integral. When using a large number of sample points, the method produces a very accurate result of the integral and provides a strong basis for simulating complex problems such as light transport. Frequently, using the necessary number of samples to reach the exact result is too computationally expensive and fewer samples are evaluated at the cost of visual noise, or variance, within the image. Importance sampling offers a means to reduce the variance by skewing the samples toward regions of the illumination integral that provide the most energy. For instance, the direction of specular reflection or a bright light source within an environment more likely represent the final value of the integral than a random sample. The variance can be reduced more efficiently by combining multiple components of the illumination integral, such as the lighting and material function, to determine where to sample, which is the principle of Multiple Importance Sampling (MIS). As an alternative to the noise in importance sampling, Filtered Importance Sampling (FIS) can provide fast integration, where the lighting environment look-ups are prefiltered to give a smoother result with a significantly smaller number of samples. Importance sampling, MIS and FIS have various practical implications. In this quarter-day course, we cover the necessary background for using Monte Carlo-based techniques for direct lighting and explain how various visual effects companies use these shading methods in their production pipelines.
Magnetic currents representing magnetomotive force for magnetic field computation

This paper proposes a formulation for magnetomotive force (MMF) employing the magnetic currents. Our formulation introduces the classical MMF into the modern magnetic field computation. The rotation of electric field represents the external magnetic field due to impressed voltage. We demonstrate a magnetic field computation of toroidal inductors by finite elements. Comparison with the computed result using the Biot-Savart law verifies our formulation. It is also verified by the experiments on the air- and magnet-core inductors.
Nanomaterial-Based Carbon Paste Electrodes for Voltammetric Determination of Naproxen in Presence of Its Degradation Products

The present work describes a novel, simple, and fast electroanalytical methodology for naproxen (NAP) determination in pharmaceutical formulations and biological fluids in the presence of its degradation products. Carbon paste electrodes (CPEs) modified with different carbon nanomaterials, namely, glassy carbon powder (GCE), multiwall carbon nanotubes (MWCNTs), single-walled carbon nanotubes (SWCNTs), graphene nanosheets (Gr), and graphene oxides (GO) were tested. Comprehensive studies were performed on the electrode matrix composition including the nature of the pasting liquids, pH, carbon nanomaterials, and mode of electrode modification. Two anodic oxidation peaks were recorded at 0.890 and 1.18 V in 1 × 10−1 mol·L−1 phosphate buffer solution at pH 6. Oxidation of naproxen (NAP) is an irreversible diffusion-controlled process. Calibration plots were rectilinear in the concentration ranging from 0.067 to 1.0 µg·mL−1 with correlation coefficient 0.9979. Photodegradation of NAP resulted in disappearance of the oxidation peak at 1.18 V, allowing simultaneous determination of NAP in presence of its degradation product. The achieved high sensitivity and selectivity suggest the application of the proposed protocol for naproxen determination in pharmaceutical preparations and human blood plasma.