Miracle-dz/UniFAR-FTUs huggingface dataset:
Ligand-specific conformational change of the G-protein–coupled receptor ALX/FPR2 determines proresolving functional responses

Significance Inflammation is a crucial host defense response but can cause chronic disease if unregulated. Several endogenous anti-inflammatory and proresolving circuits balance and modulate inflammation, including a mechanism centered on the formyl peptide receptor (FPR) family. One receptor, ALX/FPR2, recognizes both proinflammatory and proresolving signals. We have investigated this unusual molecular mechanism finding that anti-inflammatory, but not proinflammatory signals, activate homodimers of this receptor. This triggers intracellular changes culminating in the release of anti-inflammatory mediators such as IL-10. Heterodimers of ALX with other FPR receptors can transduce proapoptotic signals. These results explain how both the development and resolution of inflammation may be integrated by the same receptor system and show how drugs can be developed that have only anti-inflammatory effects. Formyl-peptide receptor type 2 (FPR2), also called ALX (the lipoxin A4 receptor), conveys the proresolving properties of lipoxin A4 and annexin A1 (AnxA1) and the proinflammatory signals elicited by serum amyloid protein A and cathelicidins, among others. We tested here the hypothesis that ALX might exist as homo- or heterodimer with FPR1 or FPR3 (the two other family members) and operate in a ligand-biased fashion. Coimmunoprecipitation and bioluminescence resonance energy transfer assays with transfected HEK293 cells revealed constitutive dimerization of the receptors; significantly, AnxA1, but not serum amyloid protein A, could activate ALX homodimers. A p38/MAPK-activated protein kinase/heat shock protein 27 signaling signature was unveiled after AnxA1 application, leading to generation of IL-10, as measured in vitro (in primary monocytes) and in vivo (after i.p. injection in the mouse). The latter response was absent in mice lacking the ALX ortholog. Using a similar approach, ALX/FPR1 heterodimerization evoked using the panagonist peptide Ac2-26, identified a JNK-mediated proapoptotic path that was confirmed in primary neutrophils. These findings provide a molecular mechanism that accounts for the dual nature of ALX and indicate that agonist binding and dimerization state contribute to the conformational landscape of FPRs.
Consumerism in Sustainable Tourism: A Survey of UK Consumers

This article presents the results of a survey of tourism consumers from the Destinations Travel Show in the UK in 2000. Four hundred and eleven tourism consumers were interviewed over four days at the show on the type of information that they used in the selection of their holiday destination. This article posits that the power of the consumer can be a major force for progress towards greater sustainability by the tourism industry, acting as a rationale for change, which is often missing from more traditional planning, management or regulatory techniques. The research shows consumers are already making decisions based on environmental, social and economic quality for day-to-day products and are keen to transfer these habits to the purchase of tourism products. Recommendations are made, highlighting the need for the tourism industry to capitalise on this demand for a wider range of product information and so promote moves towards greater levels of sustainability in the industry.
Similarity based optimization context awareness in remote healthy service

Context awareness service is one of the key features in ubiquitous computing system. In heterogeneous pervasive computing system, effective context modeling and reasoning are important to enable the collaboration and distributed reasoning among the agents. The effectiveness of the previous approaches for distributed reasoning significantly degrades when a large number of agents are involved. In order to solve this problem we propose a layered context model facilitating distributed reasoning. We also propose an similarity based context reasoning to optimize system performance. The performance of the proposed scheme is verified in remote health service, and it shows that the trust of reasoning outcome is greatly enhanced and the data traffic is effectively reduced.
Equality Bargaining: Where, Who, Why?

It is common to identify a role for trade unions in combating sex inequality at work through collective bargaining. This article uses a survey of paid union officers to identify the context in which equality bargaining by unions is likely to occur, using the specific issue of bargaining on equal pay. It concludes that equality bargaining is a function of women’s voice within unions, the characteristics and preferences of bargainers themselves and of a favourable public policy environment. Bargaining on equal pay is also more likely in centralized negotiations that cover multiple employers.
The effects of listening agent in speech-based on-line test system

Speech-based test is a one of the best ways to train students' level of proficiency. However, in e-learning it is difficult for students to maintain their concentration when speaking to a monitor. We propose speech-based online test system employing a human-likely embodied graphical agent as a listener. The developed system consists of three modules, user recognition, agent's non-verbal behavior realization, and learning controller. The system is developed with HTML5, Javascript, and WebGL, so that it should be compatible with any modern Web-browsers. To investigate the effects of the use of the listener agent in speech-based test on learning efficacy and naturalness of speech, we will conduct a one-way between-subject design experiment with three groups. In experimental group, participants speech to a listening agent with listener behavior depicted on a monitor during a speech-based test. On the other hand, in first control group, participants take the test with the listening agent but the agent does not move while the participants' speech. In second control group, participants take the test without the listening agent. Then, the result of listening scores will be statistically tested.
Rational Pensions for Irrational People: Behavioral Science Lessons for the Netherlands

This paper examines some of the main behavioral patterns that determine personal saving, investment, and retirement decisions, and considers ways to move them in a socially desirable direction. We pay special attention to factors contributing to people making choices that are not in line with their preferences. We present empirical evidence from other countries and consider their implications for proposed changes to the system in the Netherlands. The paper makes various suggestions for improvement regarding both the institutional design and product design for pension saving in the Netherlands that could be adopted in the context of recent proposals for a structural change of the pension system. In doing so it pays attention to both occupational pensions (“the second layer”) and pensions for the self employed. A comparison is made with the recommendations of the Goudswaard Committee.
Deriving health utilities from the MacNew Heart Disease Quality of Life Questionnaire

Introduction: Quality of life is included in the economic evaluation of health services by measuring the preference for health states, i.e. health state utilities. However, most intervention studies include a disease-specific, not a utility, instrument. Consequently, there has been increasing use of statistical mapping algorithms which permit utilities to be estimated from a disease-specific instrument. The present paper provides such algorithms between the MacNew Heart Disease Quality of Life Questionnaire (MacNew) instrument and six multi-attribute utility (MAU) instruments, the Euroqol (EQ-5D), the Short Form 6D (SF-6D), the Health Utilities Index (HUI) 3, the Quality of Wellbeing (QWB), the 15D (15 Dimension) and the Assessment of Quality of Life (AQoL-8D). Methods: Heart disease patients and members of the healthy public were recruited from six countries. Non-parametric rank tests were used to compare subgroup utilities and MacNew scores. Mapping algorithms were estimated using three separate statistical techniques. Results: Mapping algorithms achieved a high degree of precision. Based on the mean absolute error and the intra class correlation the preferred mapping is MacNew into SF-6D or 15D. Using the R squared statistic the preferred mapping is MacNew into AQoL-8D. Implications for research: The algorithms reported in this paper enable MacNew data to be mapped into utilities predicted from any of six instruments. This permits studies which have included the MacNew to be used in cost utility analyses which, in turn, allows the comparison of services with interventions across the health system.
The tragedy of the cocoa pod: rent-seeking, land and ethnic conflict in Ivory Coast

Many people have been surprised by the eruption of ethnic conflict and civil war in Ivory Coast. The country had gained a reputation as a relatively stable and economically prosperous agricultural republic in a region known for ethnic conflict, economic decline and civil war. The underlying factors that have led to the ethnic violence, the flight of immigrants from neighbouring countries, and the division of the country into a predominantly Muslim north and largely Christian south have been known for some time. The country's property rights regime that encouraged easy access to a forest rent – as long as cheap migrant labour and virgin forested land were available – was a recipe for future conflict. As available land declined and labour costs increased, a cycle of sharpening conflicts over these assets contributed to the current situation of ethno-regional division and civil war.
Speed estimation and compensation for harmonics in sensorless wound rotor synchronous machines

The conventional approach to control synchronous machines without a motion sensor in medium and high speed range is model-based and evaluates the electromotive force (EMF). In this case the information about the rotor position is included in the fundamental wave of the EMF and therefore the presence of harmonics will distort the estimation result. In order to compensate for harmonics information about their frequency is a prerequisite. In the case of wound rotor synchronous machines it is possible to obtain an accurate estimate of the speed by tracking the effect of the stator slots in the rotor current. For the rotor winding the stator slots represent a position dependent reluctance variation and therefore the rotor current will contain a harmonic component which is related to the rotor speed. The frequency of this harmonic component is tracked using an adaptive tracking filter and is then used to compensate for harmonics in the stator currents. The compensation is done by using adaptive resonant controllers. The suggested approach is verified experimentally by a highly utilized machine which is designed for automotive traction applications and features an extra-low voltage DC-link.
Redox cycling of anthracyclines by cardiac mitochondria. I. Anthracycline radical formation by NADH dehydrogenase.

In the present study we have used beef heart submitochondrial preparations (BH-SMP) to demonstrate that a component of mitochondrial Complex I, probably the NADH dehydrogenase flavin, is the mitochondrial site of anthracycline reduction. During forward electron transport, the anthracyclines doxorubicin (Adriamycin) and daunorubicin acted as one-electron acceptors for BH-SMP (i.e. were reduced to semiquinone radical species) only when NADH was used as substrate; succinate and ascorbate were without effect. Inhibitor experiments (rotenone, amytal, piericidin A) indicated that the anthracycline reduction site lies on the substrate side of ubiquinone. Doxorubicin and daunorubicin semiquinone radicals were readily detected by ESR spectroscopy. Doxorubicin and daunorubicin semiquinone radicals (g congruent to 2.004, signal width congruent to 4.5 G) reacted avidly with molecular oxygen, presumably to produce O2-, to complete the redox cycle. The identification of Complex I as the site of anthracycline reduction was confirmed by studies of ATP-energized reverse electron transport using succinate or ascorbate as substrates, in the presence of antimycin A or KCN respiratory blocks. Doxorubicin and daunorubicin inhibited the reduction of NAD+ to NADH during reverse electron transport. Furthermore, during reverse electron transport in the absence of added NAD+, doxorubicin and daunorubicin addition caused oxygen consumption due to reduction of molecular oxygen (to O2-) by the anthracycline semiquinone radicals. With succinate as electron source both thenoyltrifluoroacetone (an inhibitor of Complex II) and rotenone blocked oxygen consumption, but with ascorbate as electron source only rotenone was an effective inhibitor. NADH oxidation by doxorubicin during BH-SMP forward electron transport had a KM of 99 microM and a Vmax of 30 nmol X min-1 X mg-1 (at pH 7.4 and 23 degrees C); values for daunorubicin were 71 microM and 37 nmol X min-1 X mg-1. Oxygen consumption at pH 7.2 and 37 degrees C exhibited KM values of 65 microM for doxorubicin and 47 microM for daunorubicin, and Vmax values of 116 nmol X min-1 X mg-1 for doxorubicin and 114 nmol X min-1 X mg-1 for daunorubicin. In marked contrast with these results, 5-iminodaunodrubicin (a new anthracycline with diminished cardiotoxic potential) exhibited little or no tendency to undergo reduction, or to redox cycle with BH-SMP. Redox cycling of anthracyclines by mitochondrial NADH dehydrogenase is shown, in the accompanying paper (Doroshow, J. H., and Davies, K. J. A. (1986) J. Biol. Chem. 261, 3068-3074), to generate O2-, H2O2, and OH which may underlie the cardiotoxicity of these antitumor agents.
An Examination of Changes in Residents' Perceptions of Tourism Impacts Over Time: The Impact of Residents' Socio-demographic Characteristics

This study investigates whether there has been a change in residents' perceptions of various tourism impacts over time and the impact of residents' socio-demographic characteristics on those changes. A survey of local residents (n = 732 in 2004 and n = 590 in 2009) was carried out for collecting the data for this study which was then followed by focus groups, conducted in 2012, consisting of academics, local residents and the tourism experts to validate the outcome from the survey. The results suggest that there has been significant increase in the level of agreement of the Sunshine Coast residents during this period on some of the tourism impacts such as job creation, attracting more investment, providing more business for local people and more cultural exchange between tourists and residents. The results also suggest that there is a significant decline in their perceptions of tourism negatively affecting the way of living of local residents and likelihood of residents suffering from living in a tourism destination. The residents also feel that there will be a decline in parks and other recreational areas for local residents with growth in tourism. The study also found that demographic variables such as age, gender, income, education and occupation are likely to influence residents' perceptions of tourism impact over time. Implications for future tourism development plans for the region are discussed.
How Plausible is the Consensus Projection of Oil Below $25 and Persian Gulf Oil Capacity and Output Doubling by 2020?

A two-part consensus in three recent long-term projections of the world oil market states: oil's price will stay below $25 (1999 $/barrel), and OPEC oil capacity and production will increase rapidly over the next two decades to unprecedented levels, more than doubling in the Persian Gulf by 2020. Such are the projections by the International Energy Agency (IEA), by the Energy Information Administration of the U.S. Department of Energy (DOE), and in The New Economy of Oil by John Mitchell and others. Yet such projections are not based on behavioral analysis of Gulf countries' decisions; they are merely the calculated residual demand for OPEC oil, the difference between projected world oil demand and non-OPEC supply, given some assumed price-path. Such projections by IEA and DOE are implausible because they rely on supply behavior by Gulf producers that is not in their own self-interest. The DOE projections of world oil prices could be reasonable, but only if world oil demand and/or non-OPEC supply are much more price-responsive than are represented in their numerical projections. Using an updated version of the model from Gately (1995), I show that the effect of greater price-responsiveness is to make faster output growth - not higher prices - the reliable path to higher OPEC revenue. I also demonstrate the effects of uncertainty about several key parameters (such as price and income elasticities) upon model results when parameter values are randomly sampled 500 times.
Molecular alterations in partially-encapsulated or well-circumscribed follicular variant of papillary thyroid carcinoma.

BACKGROUND Studies have described an encapsulated and an infiltrative form of the follicular variant of papillary thyroid carcinoma (FVPTC). Encapsulated FVPTCs have been reported to have virtually no recurrence risk or metastatic potential and to harbor RAS mutations but not BRAF mutations. In contrast, infiltrative tumors have significant metastatic potential, a risk of recurrence, and a BRAF mutation frequency of approximately 25%. In our experience, a substantial number of FVPTCs are neither fully encapsulated nor infiltrative, but instead are partially encapsulated (PE) or well circumscribed (WC). We have previously reported that PE/WC FVPTCs behave in an indolent fashion similar to encapsulated tumors. The purpose of the current study was to evaluate the molecular alterations in PE/WC FVPTC. METHODS We identified 28 PE/WC FVPTCs resected consecutively at our institution. Targeted mutation analysis of 41 genes including members of the RAS and RAF families was performed on DNA extracted from formalin-fixed, paraffin-embedded blocks using single-base extension chemistry and mass spectrometry. RESULTS Lymph node metastases were absent in all cases with sampled lymph nodes, and no patients developed tumor recurrences (median follow-up time, 72.8 months). Overall, 13 cases (46%) harbored RAS mutations, including seven (25%) with NRAS mutations (p.Gln61Arg) and six (21%) with HRAS mutations (five had p.Gln61Arg and one had a p.Gln61Lys substitution). No PE/WC FVPTCs had BRAF mutations. CONCLUSIONS The results of this study confirm our previous finding that PE/WC FVPTCs pursue an indolent clinical course. Additionally, we found that PE/WC tumors have a similar molecular profile to that of encapsulated FVPTCs with frequent RAS mutations (46%) and no BRAF mutations. These molecular results provide further evidence that PE/WC and encapsulated FVPTCs are biologically similar and should be distinguished from more aggressive infiltrative FVPTCs.
Warm–cold colonization: response of oaks to uplift of the Himalaya–Hengduan Mountains

Clarifying the relationship between distribution patterns of organisms and geological events is critical to understanding the impact of environmental changes on organismal evolution. Quercus sect. Heterobalanus is now distributed across the Himalaya–Hengduan Mountains (HHM) and warm lowland in East China, yet how the distribution patterns of this group changed in response to the HHM uplift remains largely unknown. This study examines the effect of tectonic events in the HHM region on the oaks, providing a biological perspective on the geological history of this region. Fifty‐six populations of Quercus sect. Heterobalanus were genotyped using four chloroplast DNA regions and nine nuclear simple sequence repeat loci to assess population structure and diversity, supplemented by molecular dating and ancestral area reconstructions. The underlying demographic dynamics were compared using ecological niche models of the species distributions during the last glacial maximum and the present. These analyses illustrate that Quercus sect. Heterobalanus diversified as the HHM uplifted and climatic cooling during the mid‐Miocene, colonizing the cold habitats from warm broadleaf mixed forests. Lineages in cold highlands and warm lowlands have diverged as a consequence of local adaptation to diverging climates since the late Miocene. Our results suggest that continuous uplift of the HHM in the late Miocene to early Pliocene accompanied by simultaneous cooling triggered the differentiation of oaks. The biogeography of Quercus sect. Heterobalanus illuminates the geological events responsible for the modern‐day HHM.
Is lowest-low fertility in Europe explained by the postponement of childbearing?

In 2001, more than half of Europe's population lived in countries with a total fertility rate (TFR) at or below 1.3. Use of the adjusted TFR proposed by Bongaarts and Feeney, which takes into account the effects of the ongoing fertility postponement, changes the European fertility map considerably. All 27 countries analyzed had adjusted TFRs in 1995-2000 above 1.4. Thus, the "lowest-low" fertility in Europe may be interpreted as a temporary consequence of the increasing age at motherhood. However, substantial regional differences in fertility level across Europe persist even when the differential pace of fertility postponement is taken into account. The estimated adjusted TFRs in Europe (1.63) and in the 25-member European Union (1.71) contrast with the TFR levels of 1.40 and 1.46, respectively. These seemingly small differences have vastly different implications in terms of the potential long-term pace of population decline. Copyright 2004 The Population Council, Inc.
Rod and Cone Pathways in the Inner Plexiform Layer of Cat Retina

In cat retina, rod bipolar terminials do not synapse on ganglion cells but on two types of amacrine cell (types I and II). Cone bipolars synapse directly on ganglion cells and on type I amacrines. The type II amacrine appears to play a special internuncial role between bipolars and ganglion cells in the rod system.
Banking reform in transition countries

In reforming the financial sector in transition economies, one important debate is whether governments should try to reform existing state-owned banks (the rehabilitation approach) or whether a new private banking system should be allowed to emerge (a new entry approach). Or should there be a mix of the two approaches, in which the state bank activities are restricted while a parallel private banking system develops? The authors' cross-country comparison of banks' institutional development in 25 transitional economies suggests that progress can be faster under the new entry approach, especially relative to initial conditions. Progress under the rehabilitation approach appears to be inhibited by poor incentives. In most countries, even those with a good banking infrastructure and a large segment of good banks, a two track process has evolved, with differences between weak and strong banks. Weak banks have moved little beyond central planning. Regression estimates suggest that slow progress of weak banks is associated with: cover concentration, government preferential treatment, and limited new banks entry. The causality direction is often unclear. Policies and structural conditions can affect bank quality. The role of banks will remain limited in many transition economies due to weak legal infrastructures, much uncertainty and inside information, and problems associated with highly leveraged financial intermediaries - including fraud, political interference, and implicit guarantees. In the short run, self-finance and intermediation among enterprises and through nonbank financial institutions may prevail.