The microbiome as a source of new enterprises and job creation: Considering clinical faecal and synthetic microbiome transplants and therapeutic regulation

Modulation of the human immune system has become the focus for several novel approaches to treat conditions related to immune dysregulation, chronic infections and oncology. The human gut microbiome is being recognized as a key factor associated with the innate immune response, and exploring it has resulted in the identification of leads for therapeutics to treat conditions related to immune dysregulation and chronic infections, such as asthma, allergic rhinitis, eczema, IBD, IBS, Crohn’s disease, chronic intestinal infections and various forms of food allergies like allergies to peanuts, shellfish and dairy products. A number of companies are active in this area and developing therapeutic faecal microbiome transplants [FMT (Van Nood et al., 2013)] and defined microbial consortia to treat infections with Clostridium difficile, of which the products of Rebiotix and Seres Therapeutics are among the most advanced. Overall, synthetic microbiomes (designer formulations) for transplants are preferred over FMT to attain desired/demanded standardization and safety standards, mode of action understanding and acceptability by regulatory agencies (Bojanova and Bordenstein, 2016). However, recent clinical trials to treat chronic infections with C. difficile using FMT were successful (Van Nood et al., 2013), while a defined microbial consortium performed below expectation. Therefore, additional research is required to better understand the critical roles and interdependencies of keystone strains in the human gut microbiome to design successful therapeutics. The majority of designer formulations for modulating the immune response revolve around human-derived butyrate-producing bacterial species that belong to the Clostridia classes IV and XVIa to induce the accumulation of regulatory T cells that lead to the control of inflammation, a decrease in the secretion of a proinflammatory cytokine, or an enhanced secretion of an antiinflammatory cytokine by a population of human peripheral blood mononuclear cells. The best-documented example of this approach is the work by Kenya Honda around a 17 species Clostridium strain consortium (Atarashi et al., 2011, 2013), VE202, which is currently being developed by companies like Vedanta Biosciences and Johnson & Johnson. Thus far, such probiotic formulations have proven useful in the treatment of immune disorders in only a subset of patients, further supporting the case for the need to better understand the interdependencies and interactions among keystone strains to improve engrafting and performance of probiotic formulations based on synthetic microbiomes. The complexity of the human gut microbiome has limited the development of microbiome-based therapeutics. This has prompted several efforts to develop predictive models to study the critical interdependencies of microbiome keystone species and the impact of host–microbiome interactions in specific diseases. Recent examples of such modelling systems include CASINO – Community and Systems-level Interactive Optimization (Shoaie et al., 2015) – and AGORA – Assembly of Gut Organisms through Reconstruction and Analysis (Magn usd ottir et al., 2016). It is expected that in the near future, predictive modelling will change the way microbiome research and development is being carried out, not just for microbial therapeutics, but also in adjacent areas, such as immunotherapy drugs for cancer treatment. Examples of processes, which are constrained by costs and time for experimental validation and will benefit from predictive modelling, include mode of action understanding, finding new indications, add-on/adjunct therapies, root cause analysis, understanding of adverse events, optimized engraftment, effects of diet, secondary prevention (comorbidity), identification of predictive biomarkers, optimized trial design, detailed cohort studies and sample size extrapolation. An example of a start-up company that is at the forefront of using predictive modelling for every aspect of their R&D platform is Gusto Global: their modelling platform enables a significant (100-fold or more) in silico reduction of experimental permutations for hypothesisdriven experimental confirmation, mode of action understanding and product optimization. This provides a substantial opportunity for rapid optimization of existing