Miracle-dz/UniFAR-FTUs huggingface dataset:
Measles Virus Spread and Pathogenesis in Genetically Modified Mice

ABSTRACT Attenuated Edmonston measles virus (MV-Edm) is not pathogenic in standard mice. We show here that MV-Edm inoculated via the natural respiratory route has a limited propagation in the lungs of mice with a targeted mutation inactivating the alpha/beta interferon receptor. A high dose of MV-Edm administered intracerebrally is lethal for about half of these mice. To study the consequences of the availability of a high-affinity receptor for MV propagation, we generated alpha/beta interferon-defective mice expressing human CD46 with human-like tissue specificity. Intranasal infection of these mice with MV-Edm resulted in enhanced spread to the lungs and more prominent inflammatory response. Virus replication was also detected in peripheral blood mononuclear cells, the spleen, and the liver. Moreover, intracerebral inoculation of adult animals with low MV-Edm doses caused encephalitis with almost inevitably lethal outcome. We conclude that in mice alpha/beta interferon controls MV infection and that a high-affinity receptor facilitates, but is not strictly required for, MV spread and pathogenesis.
A Bizarre Virtual Trainer Outperforms a Human Trainer in Foreign Language Word Learning

In this study, the effects that a human trainer and a pedagogical virtual agent have on the memory for words in a foreign language (L2) were investigated. In a recent study on L2 word learning, Bergmann and Macedonia (2013) cued participants to memorize novel words both audiovisually and by performing additional gestures. The gestures were performed by both a human and a virtual trainer. In some of the tests, the virtual agent had a greater positive influence on memory performance than the human trainer. In order to determine why the agent was a better trainer than the human, 18 naive subjects were invited to rate the gestures performed by both trainers. Furthermore, participants were asked to evaluate their perception of the human and the agent. It was hypothesized that the gestures performed by the agent would be more peculiar than those by the human and possibly attract greater attention. It was also hypothesized that the agent’s personality might be more appealing than that of the human. The results showed that the agent’s gestures were perceived as less natural than those of the human. This might have triggered greater attention and/ or more emotional involvement of the participants. The perception of both trainers as “personalities” did not differ, with the exception of a few traits for which the human trainer was considered to be better. Altogether, because of the peculiar gestures it made and because of its looks, the agent may have been perceived as bizarre. Therefore, he might have induced the bizarreness effect in the memory for words. KeywordsVirtual Agen; Evaluatio; Foreign Language; Learning
Prognosis and adjuvant treatment effects in selected breast cancer subtypes of very young women (<35 years) with operable breast cancer.

BACKGROUND There is limited knowledge about prognosis of selected breast cancer subtypes among very young women. PATIENTS AND METHODS We explored patterns of recurrence by age according to four immunohistochemically defined tumor subtypes: Luminal A and Luminal B (estrogen receptor positive and/or progesterone receptor positive and either human epidermal growth factor receptor 2 (HER2) positive and/or high Ki-67), HER2-positive (and) endocrine receptor absent and Triple Negative, in 2970 premenopausal patients with pT1-3, pN0-3 and M0 breast cancer. RESULTS Patients <35 years of age (315, 11%) presented a significantly increased risk of recurrence and death [hazards ratio (HR) = 1.65, 95% confidence interval (CI) 1.30-2.10 and HR = 1.78, 95% CI 1.12-2.85, respectively] when compared with older patients (2655, 89%) with similar characteristics of disease. This was true considering patients with Luminal B [HR = 1.62, 95% CI 1.21-2.18 for disease-free survival (DFS) and HR = 2.09, 95% CI 0.96-4.53 for overall survival (OS)] and with Triple Negative (HR = 2.04, 95% CI 1.11-3.72 for DFS and HR = 2.20, 95% CI 1.10-4.41 for OS) breast cancer, observing the highest risk of recurrence in the younger patients with HER2-positive breast cancer (HR = 2.37, 95% CI 1.12-5.02) when compared with older patients. CONCLUSIONS Very young patients with Triple Negative, Luminal B or HER2-positive breast cancer have a worse prognosis when compared with older patients with similar characteristics of disease.
Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants.

### Calcium Signaling and Calcium Sensors: A General Paradigm Many extracellular signals, including light, biotic, and abiotic stress factors, elicit changes in cellular Ca2+ concentration in plants ([Trewavas and Knight, 1994][1]; [Bush, 1995][2]; [Braam et al., 1997][3]; [McAinsh et al., 1997][4
Asynchronous Anytime Sequential Monte

We introduce a new sequential Monte Carlo algorithm we call the particle cascade. The particle cascade is an asynchronous, anytime alternative to traditional sequential Monte Carlo algorithms that is amenable to parallel and distributed implementations. It uses no barrier synchronizations which leads to improved particle throughput and memory efficiency. It is an anytime algorithm in the sense that it can be run forever to emit an unbounded number of particles while keeping within a fixed memory budget. We prove that the particle cascade provides an unbiased marginal likelihood estimator which can be straightforwardly plugged into existing pseudo-marginal methods.
The antimicrobial activity of nanoparticles: present situation and prospects for the future

Nanoparticles (NPs) are increasingly used to target bacteria as an alternative to antibiotics. Nanotechnology may be particularly advantageous in treating bacterial infections. Examples include the utilization of NPs in antibacterial coatings for implantable devices and medicinal materials to prevent infection and promote wound healing, in antibiotic delivery systems to treat disease, in bacterial detection systems to generate microbial diagnostics, and in antibacterial vaccines to control bacterial infections. The antibacterial mechanisms of NPs are poorly understood, but the currently accepted mechanisms include oxidative stress induction, metal ion release, and non-oxidative mechanisms. The multiple simultaneous mechanisms of action against microbes would require multiple simultaneous gene mutations in the same bacterial cell for antibacterial resistance to develop; therefore, it is difficult for bacterial cells to become resistant to NPs. In this review, we discuss the antibacterial mechanisms of NPs against bacteria and the factors that are involved. The limitations of current research are also discussed.
Effects of homocysteine lowering with B vitamins on cognitive aging: meta-analysis of 11 trials with cognitive data on 22,000 individuals12345

Background: Elevated plasma homocysteine is a risk factor for Alzheimer disease, but the relevance of homocysteine lowering to slow the rate of cognitive aging is uncertain. Objective: The aim was to assess the effects of treatment with B vitamins compared with placebo, when administered for several years, on composite domains of cognitive function, global cognitive function, and cognitive aging. Design: A meta-analysis was conducted by using data combined from 11 large trials in 22,000 participants. Domain-based z scores (for memory, speed, and executive function and a domain-composite score for global cognitive function) were available before and after treatment (mean duration: 2.3 y) in the 4 cognitive-domain trials (1340 individuals); Mini-Mental State Examination (MMSE)–type tests were available at the end of treatment (mean duration: 5 y) in the 7 global cognition trials (20,431 individuals). Results: The domain-composite and MMSE-type global cognitive function z scores both decreased with age (mean ± SE: −0.054 ± 0.004 and −0.036 ± 0.001/y, respectively). Allocation to B vitamins lowered homocysteine concentrations by 28% in the cognitive-domain trials but had no significant effects on the z score differences from baseline for individual domains or for global cognitive function (z score difference: 0.00; 95% CI: −0.05, 0.06). Likewise, allocation to B vitamins lowered homocysteine by 26% in the global cognition trials but also had no significant effect on end-treatment MMSE-type global cognitive function (z score difference: −0.01; 95% CI: −0.03, 0.02). Overall, the effect of a 25% reduction in homocysteine equated to 0.02 y (95% CI: −0.10, 0.13 y) of cognitive aging per year and excluded reductions of >1 mo per year of treatment. Conclusion: Homocysteine lowering by using B vitamins had no significant effect on individual cognitive domains or global cognitive function or on cognitive aging.
Brittle, ductile, paste-like behaviors and distinct necking of double network gels with enhanced heterogeneity

We propose a simple method to obtain double network (DN) hydrogels containing 85–90wt% water with various kinds of mechanical performance by taking advantage of the heterogeneity of the first network: By synthesizing the first network in a poor solvent that enhancing the heterogeneity of the network, the DN gel exhibits rich mechanical behavior: from brittle, ductile (necking), to paste-like. For a proper heterogeneity, a distinct necking deformation with extensibility of about 20 times is observed for post-necking gels. The necking gels have a high initial modulus (0.1–0.5 MPa) and high mechanical toughness (nominal fracture stress: 1 MPa), both values are similar to that of living tissues.
An Increased Frequency in HLA Class I Alleles and Haplotypes Suggests Genetic Susceptibility to Influenza A (H1N1) 2009 Pandemic: A Case-Control Study

Background The influenza A H1N1/09 pandemic infected a small number of exposed individuals, which suggests the involvement of genetic factors. There are scarce data available on classical HLA class I association with the influenza A H1N1/09 pandemic. Methods We analyzed the frequency of classical HLA class I alleles and haplotypes in A H1N1/09 influenza in a case-control study including 138 influenza patients (INF-P) and 225 asymptomatic healthy contacts (INF-C) simultaneously recruited. HLA class I typing was performed by high-resolution sequence-based typing method. Results Our analysis revealed higher frequency of C∗07:02:01, B∗39:06:02, C∗03:02:01, B∗44:03:01, B∗51:01:05, and B∗73:01 (p < 0.05; OR = 1.84–9.98) and of two haplotypes—A∗68:01:02-C∗07:02:01 (p = 1.05E − 05; OR = 23.99) and B∗35:01:01-C∗07:02.01 (p = 4.15E − 04, OR = 2.15)—in A H1N1/09 influenza subjects. A∗68:01:01 was exclusively present only in the INF-P group (5/138). A decrease in the frequency of C∗03:03:01, A∗11:01:01, B∗39:01:01, A∗24:02:01, C∗03:04:01, B∗51:01:01, and C∗07:01:01 (p < 0.05; OR = 0.12–0.52) and of haplotypes A∗02:01:01-B∗35:01:01-C∗04:01:01, A∗24:02:01-B∗35:01:01, B∗39:01:01-C∗07:02:01, and B∗40:02:01-C∗03:04:01 (p < 0.05; OR = 0.08–0.22) were observed in INF-P group. Conclusion Selective classical HLA class I allele and haplotype combinations predispose individuals towards susceptibility or protection against the influenza A H1N1/09 pandemic. This work has significant implications for accessing population transmission risk for A H1N1/09 or a similar strain breakout in the future.
Single-phase to two-phase power converter

This paper presents two single-phase to two-phase converters. The converters can supply two loads, 180 degree lagged from each other, with sinusoidal waveform and constant amplitude and frequency, even with non-linear or unbalanced loads. Also, the converters provide sinusoidal grid current and unity power factor. Furthermore, the pulsewidth modulation (PWM) techniques are discussed and implemented. Simulation and experimental results are provided to illustrate and compare the operation of the proposed systems.
dPCR: A Technology Review

Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification of target nucleic acids. Quantification by dPCR hinges on the fact that the random distribution of molecules in many partitions follows a Poisson distribution. Each partition acts as an individual PCR microreactor and partitions containing amplified target sequences are detected by fluorescence. The proportion of PCR-positive partitions suffices to determine the concentration of the target sequence without a need for calibration. Advances in microfluidics enabled the current revolution of digital quantification by providing efficient partitioning methods. In this review, we compare the fundamental concepts behind the quantification of nucleic acids by dPCR and quantitative real-time PCR (qPCR). We detail the underlying statistics of dPCR and explain how it defines its precision and performance metrics. We review the different microfluidic digital PCR formats, present their underlying physical principles, and analyze the technological evolution of dPCR platforms. We present the novel multiplexing strategies enabled by dPCR and examine how isothermal amplification could be an alternative to PCR in digital assays. Finally, we determine whether the theoretical advantages of dPCR over qPCR hold true by perusing studies that directly compare assays implemented with both methods.
In a green frame of mind: perspectives on the behavioural ecology and cognitive nature of plants

It is increasingly recognized that plants are highly sensitive organisms able to perceive, assess, learn, remember, resolve problems, make decisions and communicate with each other by actively acquiring information from their environment. If plants so efficiently control the way they interact and behave in their environment, would it be appropriate to say that they have a mind of their own? In this paper, I propose that the time is ripe for asking this and related questions about the mind of plants and suggest how we may venture into this unexplored mindscape.
Prediabetes and Adolescence—Trends, Causes, Effects, and Screening

1. Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, Choremeio Research Laboratory, First Department of Pediatrics, School of Medicine, National and Kapodistrian University of Athens, Aghia Sophia Children's Hospital, Athens, Greece; 2. Endocrine Unit, Second Department of Internal Medicine-Propaedeutic, Research Institute and Diabetes Center, National and Kapodistrian University of Athens, Attikon University Hospital, Athens, Greece
Subjective Socioeconomic Status Moderates the Association between Discrimination and Depression in African American Youth

Background: Most of the literature on the association between socioeconomic status (SES) and health is focused on the protective effects of SES. However, a growing literature suggests that high SES may also operate as a vulnerability factor. Aims: Using a national sample of African American youth, this study compared the effects of perceived discrimination on major depressive disorder (MDD) based on SES. Methods: The current cross-sectional study included 810 African American youth who participated in the National Survey of American Life-Adolescent supplement. The independent variable was perceived discrimination. Lifetime, 12-month, and 30-day MDD were the dependent variables. Age and gender were covariates. Three SES indicators (subjective SES, income, and poverty index) were moderators. We used logistic regressions for data analysis. Results: Perceived discrimination was associated with higher risk of lifetime, 12-month, and 30-day MDD. Interactions were found between subjective SES and perceived discrimination on lifetime, 12-month, and 30-day MDD, suggesting a stronger effect of perceived discrimination in youth with high subjective SES. Objective measures of SES (income and poverty index) did not interact with perceived discrimination on MDD. Conclusion: While perceived discrimination is a universally harmful risk factor for MDD, its effect may depend on the SES of the individual. Findings suggest that high subjective SES may operate as a vulnerability factor for African American youth.
Clinical Evaluation of an Image-Guided Cochlear Implant Programming Strategy

The cochlear implant (CI) has been labeled the most successful neural prosthesis. Despite this success, a significant number of CI recipients experience poor speech understanding, and, even among the best performers, restoration to normal auditory fidelity is rare. While significant research efforts have been devoted to improving stimulation strategies, few developments have led to significant hearing improvement over the past two decades. We have recently introduced image processing techniques that open a new direction for advancement in this field by making it possible, for the first time, to determine the position of implanted CI electrodes relative to the nerves they stimulate using computed tomography images. In this article, we present results of an image-guided, patient-customized approach to stimulation that utilizes the electrode position information our image processing techniques provide. This approach allows us to identify electrodes that cause overlapping stimulation patterns and to deactivate them from a patient's map. This individualized mapping strategy yields significant improvement in speech understanding in both quiet and noise as well as improved spectral resolution in the 68 adult CI recipients studied to date. Our results indicate that image guidance can improve hearing outcomes for many existing CI recipients without requiring additional surgery or the use of ‘experimental' stimulation strategies, hardware or software. i 2014 S. Karger AG, Basel
Three-phase on-board charger with three modules of single-stage interleaved soft-switching AC-DC converter

This paper proposes a new three-phase on-board charger with three modules of single-phase single-stage interleaved soft-switching ac-dc converter. Each module has single-stage structure with reduced component counts. It achieves ZVS turn-on of all switches and ZCS turn-off of all diodes. Eliminating the low frequency component in the battery charging current, the proposed converter is able to charge the battery with dc current. A balancing control algorithm is proposed to limit the excessive current through each module caused by unbalanced three-phase input voltage. A preliminary prototype of the proposed converter has been built and tested to verify the validity of the proposed concept.
Efficient Solutions to Some Transportation Problems with Applications to Minimizing Robot Arm Travel

We give efficient solutions to transportation problems motivated by the following robotics problem. A robot arm has the task of rearranging m objects between n stations in the plane. Each object is initially at one of these n stations and needs to be moved to another station. The robot arm consists of a single link that rotates about a fixed pivot. The link can extend in and out (like a telescope) so that its length is a variable. At the end of this “telescoping” link lies a gripper that is capable of grasping any one of the m given objects (the gripper cannot be holding more than one object at the same time). The robot arm must transport each of the m objects to its destination and come back to where it started. Since the problem of scheduling the motion of the gripper so as to minimize the total distance traveled is NP-hard, we focus on the problem of minimizing only the total angular motion (rotation of the link about the pivot), or only the telescoping motion. We give algorithms for two different mode.
Augmenting NO x reduction in diesel exhaust by combined plasma/ozone injection technique: a laboratory investigation

With the advent of non-thermal plasma technique for diesel exhaust treatment attention is being focused on reducing oxides of nitrogen (NO x ) both from stationary as well as automobile sources. In this work, an attempt has been made to enhance NO x reduction only by utilising a plasma/ozone injection approach without resorting to additional treatments based on catalysts/adsorbents. It was observed that cascading ozone injection with discharge plasma enhances the reduction of NO x as high as 95% for the specific energy of 123 J/l. In the long run, this plasma-only based approach may be beneficial or a possible alternative for catalyst/adsorbent based NO x treatments. The results have been discussed and a comparative analysis has been made with respect to individual plasma and ozone injection treatment.
Using Program Induction to Interpret Transition System Dynamics

Explaining and reasoning about processes which underlie observed black-box phenomena enables the discovery of causal mechanisms, derivation of suitable abstract representations and the formulation of more robust predictions. We propose to learn high level functional programs in order to represent abstract models which capture the invariant structure in the observed data. We introduce the $\pi$-machine (program-induction machine) -- an architecture able to induce interpretable LISP-like programs from observed data traces. We propose an optimisation procedure for program learning based on backpropagation, gradient descent and A* search. We apply the proposed method to two problems: system identification of dynamical systems and explaining the behaviour of a DQN agent. Our results show that the $\pi$-machine can efficiently induce interpretable programs from individual data traces.
Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

12–14 days of culturing of bone marrow (BM) cells containing various growth factors is widely used method for generating dendritic cells (DCs) from suspended cell population. Here we compared flask culture method and commercially available CD11c Positive Selection kit method. Immature BMDCs' purity of adherent as well as suspended cell population was generated in the decreasing concentration of recombinant-murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF) in nontreated tissue culture flasks. The expression of CD11c, MHCII, CD40, and CD86 was measured by flow cytometry. We found significant difference (P < 0.05) between the two methods in the adherent cells population but no significant difference was observed between the suspended cell populations with respect to CD11c+ count. However, CD11c+ was significantly higher in both adhered and suspended cell population by culture method but kit method gave more CD11c+ from suspended cells population only. On the other hand, using both methods, immature DC expressed moderate level of MHC class II molecules as well as low levels of CD40 and CD86. Our findings suggest that widely used culture method gives the best results in terms of yield, viability, and purity of BMDCs from both adherent and suspended cell population whereas kit method works well for suspended cell population.
Impedance properties of periodic linear arrays conformal to a dielectric-clad infinite PEC cylinder

Oversize materials (e.g., maps, drawings, charts) are re­ produced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. These are also available as one exposure on a standard 35mm slide or as a 17" x 23" black and white photographic print for an additional charge.