Miracle-dz/UniFAR-FTUs huggingface dataset:
The Antitumor Effects of α-Linolenic Acid

α-linolenic acid (ALA), which is a member of the n-3 polyunsaturated fatty acid (n-3 PUFA) family, has often been ignored due to a lack of information. ALA has gradually attracted increased attention due to its nutritional and medicinal advantages. Studies have shown that ALA exerts beneficial effects on a variety of diseases, including cancer. In this review, we summarize the antitumor effects of ALA in the context of cell biology, including the inhibition of proliferation, the induction of apoptosis, the inhibition of metastasis and angiogenesis, and antioxidant effects. In addition, studies have shown that ALA can be used as a drug carrier or exert positive clinical effects when combined with drugs. Therefore, the use of ALA in clinical treatments is very promising and valuable.
Green Manufacturing of Nanoparticles for Biomedical Applications

The vast majority of nanomaterials are chemically synthesized, a costly process, that is environmentally risky, and the produced nanoparticles are potentially toxic to patients. Nature-based nanomaterials, however, are proving to be much more biocompatible with lower environmental toxicity. Even though a variety of natural nanomaterials have been designed, fabrication technologies for the desired natural nanoparticles with reproducible quality, high productivity and low cost remain a challenge. My objective has been to establish strategies for the isolation, purification and characterization of nanoparticles using a production system based on green tea and fungus (Arthrobotrys oligospora) and also to develop new approaches for sustainable “green manufacture” of gold nanoparticles for biomedical applications. First, an infusion-dialysis procedure to isolate of the tea nanoparticles (TNPs) from a green tea infusion was developed and validated. The TNPs are spherical with a diameter of 100-300 nm, and have a zeta potential of -26.52 mV at pH 7.0. The TNPs enhance secretion of the cytokines and the chemokines from mouse macrophages, suggesting a potential immunostimulatory effect. As a natural nanocarrier, the TNPs are able to form complexes with doxorubicin (DOX). The DOX-loaded TNPs increase cellular DOX uptake, leading to higher cytotoxicity in cancer cells. Second, a new isolation procedure was established to purify the fungal nanoparticles (FNPs) from A. oligospora, giving two purified FNP fractions. Both purified FNPs had a reduced diameter of 100-200 nm, with glycosaminoglycan as the main constituent. The purified FNPs cause mild cytotoxicity by inducing apoptosis and regulating the cell cycle in multiple tumor cell lines and have an immunostimulatory effect. Additionally, the FNPs have an immunochemotherapeutic effect upon complexing with DOX against tumor cells. Third, a sustainable system for green manufacturing of gold nanoparticles was developed by using actively growing English ivy. The efficient uptake of the synthesized gold nanoparticles in mammalian cells provides the potential for biomedical applications. Finally, a simple one-step approach using dopamine, a monoamine neurotransmitter appearing naturally in the human brain, to synthesize highly branched gold nanoflowers (AuNFs) was developed. These AuNFs are highly biocompatible and provide high surface enhanced Roman scattering (SERS) performance.
High‐resolution quantification of earthworm calcite granules from western European loess sequences reveals stadial–interstadial climatic variability during the Last Glacial

High concentrations of calcite fossil granules produced by earthworms (ECG) have been identified in most of the stratigraphical units along the loess‐palaeosol reference sequence of Nussloch (Germany). They are particularly abundant in interstadial brown soils and in tundra gley horizons, the latter reflecting short‐term phases of aggradation then degradation of permafrost. These granules are characterized by a radial crystalline structure produced in the earthworms by specific bio‐mineralization processes. In our study, we used this biological indicator combined with 14C and OSL dating, and sedimentological parameters to characterize millennial‐time scale climatic variations recorded in loess sequences. The approach is based on high‐resolution counts of ECG throughout a 17‐m‐thick loess sequence (332 samples). Strong increases in granule and mollusc concentrations suggest warmer climate conditions during palaeosol formation phases, associated with increasing biodiversity, biological activity and vegetation cover. Decreased granule concentrations occur within primary loess deposits, indicating a strong correlation with palaeoenvironmental conditions and demonstrating the reliability of ECG concentration variations as a new palaeoenvironmental proxy. Finally, this pattern is also recorded in loess sequences located about 600 km westward in northern France demonstrating the large‐scale validity of this new palaeoclimatic proxy.
Effects of Aging and Nerve Growth Factor on Neuropeptide Expression and Cholinergic Innervation of the Rat Basolateral Amygdala

Simple Summary The basolateral amygdala (BLA) contains neurons that produce neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP). These neuropeptides are involved in the regulation of several brain functions and behaviors, and there is evidence that, in some brain regions, the acetylcholine might modulate its expression. Thus, we study if aging alters the BLA densities of NPY- and VIP-positive neurons and cholinergic varicosities. We also investigate if any potential change in these parameters might be related to insufficient trophic support provided by the neurotrophin nerve growth factor (NGF). We found that the density of NPY-positive neurons was reduced in aged rats, whereas the density of VIP-immunoreactive neurons was unchanged. The decreased expression of NPY was fully reversed by intracerebroventricular administration of NGF. The density of cholinergic varicosities was similar in adult and old rats, and the infusion of NGF to aged rats increased their density to greater than the values of adult and old rats. These results indicate that the NPY expression in the BLA is affected by aging, and also show that this age-related change can be associated with alterations in the NGF trophic support. Furthermore, the results also show that the BLA cholinergic innervation is particularly resistant to age effects. Abstract The basolateral amygdala (BLA) contains interneurons that express neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP), both of which are involved in the regulation of functions and behaviors that undergo deterioration with aging. There is considerable evidence that, in some brain areas, the expression of NPY and VIP might be modulated by acetylcholine. Importantly, the BLA is one of the brain regions that has one of the densest cholinergic innervations, which arise mainly from the basal forebrain cholinergic neurons. These cholinergic neurons depend on nerve growth factor (NGF) for their survival, connectivity, and function. Thus, in this study, we sought to determine if aging alters the densities of NPY- and VIP-positive neurons and cholinergic varicosities in the BLA and, in the affirmative, if those changes might rely on insufficient trophic support provided by NGF. The number of NPY-positive neurons was significantly reduced in aged rats, whereas the number of VIP-immunoreactive neurons was unaltered. The decreased NPY expression was fully reversed by the infusion of NGF in the lateral ventricle. The density of cholinergic varicosities was similar in adult and old rats. On the other hand, the density of cholinergic varicosities is significantly higher in old rats treated with NGF than in adult and old rats. Our results indicate a dissimilar resistance of different populations of BLA interneurons to aging. Furthermore, the present data also show that the BLA cholinergic innervation is particularly resistant to aging effects. Finally, our results also show that the reduced NPY expression in the BLA of aged rats can be related to changes in the NGF neurotrophic support.
Quercetin, a Flavonoid with Great Pharmacological Capacity

Quercetin is a flavonoid with a low molecular weight that belongs to the human diet’s phenolic phytochemicals and nonenergy constituents. Quercetin has a potent antioxidant capacity, being able to capture reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive chlorine species (ROC), which act as reducing agents by chelating transition-metal ions. Its structure has five functional hydroxyl groups, which work as electron donors and are responsible for capturing free radicals. In addition to its antioxidant capacity, different pharmacological properties of quercetin have been described, such as carcinostatic properties; antiviral, antihypertensive, and anti-inflammatory properties; the ability to protect low-density lipoprotein (LDL) oxidation, and the ability to inhibit angiogenesis; these are developed in this review.
Regulation of Disease-Resistance Genes against CWMV Infection by NbHAG1-Mediated H3K36ac

Post-translational modification of proteins plays a critical role in plant–pathogen interactions. Here, we demonstrate in Nicotiana benthamiana that knockout of NbHAG1 promotes Chinese wheat mosaic virus (CWMV) infection, whereas NbHAG1 overexpression inhibits infection. Transcriptome sequencing indicated that a series of disease resistance-related genes were up-regulated after overexpression of NbHAG1. In addition, cleavage under targets and tagmentation (Cut&Tag)-qPCR results demonstrated that NbHAG1 may activate the transcription of its downstream disease-resistance genes by facilitating the acetylation level of H3K36ac. Therefore, we suggest that NbHAG1 is an important positive regulator of resistance to CWMV infestation.
Comparison and Investigation of AI-Based Approaches for Cyberattack Detection in Cyber-Physical Systems

The demand for cyber-physical systems (CPSs) has recently increased in various domains, such as smart grids, intelligent transportation, and critical infrastructure. The massive data networks and communication layers generated make CPSs vulnerable to threats and cyberattacks. To mitigate these threats, artificial intelligence (AI) approaches are employed. However, AI models struggle to keep up with the constantly changing attack landscape. This study investigates the application of extreme gradient boosting (XGBoost) and long-short-term memory (LSTM) AI models for cyberattack detection in a CPS. Accuracy, precision, recall, and the F1-score validate the approach as evaluation metrics. The methods were tested on a gas pipeline industrial control system dataset and other benchmark datasets, such as NetML-2020 and IoT-23, which contain various cyberattacks. The performance of the two methods was found to be better than other models such as support vector machine (SVM) and artificial neural networks (ANN) on several evaluation metrics. Finally, we present recommendations for future research.
Effect of Early Warning Score on Length of Stay and Mortality of Non-Surgical Patients in the Intensive Care Room at Dr. Moewardi Hospital Surakarta

Background: One of the efforts to improve the quality of life and patient safety in hospitals is the use of the Early Warning Score (EWS). The purpose of this study was to analyze the effect of EWS on the length of stay and mortality of non-surgical patients in the intensive care unit of Dr. Moewardi Hospital Surakarta.Subjects and Method: This study is an observational study conducted using a prospective cohort study. A purposive sampling study conducted on June 10 - July 10 was carried out by taking a sample of 120 non-surgical patients in the ICU of Dr. Moewardi Hopital, Surakarta with inclusion and exclusion criteria. This study chose the early warning score as the independent variable and length of stay and mortality as the dependent variable. The method of collecting data on EWS, length of stay, and mortality was using an observation sheet filled out by the researcher. This study assessed differences in baseline characteristics and outcomes using the Chi-Square test and one-way analysis of variance (ANOVA). Results: There is a statistically significant effect of EWS on length of stay (p = 0.024). EWS also has a statistically significant effect on mortality p<0.001. Conclusion: There is an effect of EWS on the length of stay and mortality of non-surgical patients.Keywords: EWS, length of stay, mortality, non-surgical patients, intensive careCorrespondence: Luthfiana Nadhiifa Khoirunnisa. Faculty of Medicine, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia. Email: luthfiana@student.uns. ac.id. Mobile: +62 82111170000.Indonesian Journal of Medicine (2023), 08(03): 310-316https://doi.org/10.26911/theijmed.2023. 08.03. 08
Assessing nutrient management scenarios at the system level

ii ACKNOWLEDGMENTS . iv TABLE OF CONTENTS . v LIST OF TABLES . vii LIST OF FIGURES . ix CHAPTER 1: INTRODUCTION . 1 1.1: Modeling Irrigated Agriculture . 1 1.2: Modeling Urban Stormwater . 2 1.3: Modeling WWTP Technologies and Water Management Practices . 3 1.4: Natural Background Loads . 3 1.5: Ambient Water Quality . 4 1.6: Cost and Equity . 4 CHAPTER 2 : ASSESSING CONSERVATION EFFECTS OF AGRICULTURAL MANAGEMENT PRACTICES IN IRRIGATED RIVER BASINS . 6 2.1: Introduction . 6 2.2: Methods . 8 2.2.1: Study Watershed . 8 2.2.2: Model Description . 9 2.2.3: Field Observations. 11 2.2.4: Model Calibration . 12 2.2.4: Scenario Analysis . 14 2.2. 5: Description of Scenarios . 16 2.3: Results and Discussion . 21 2.4: Summary and Conclusions . 27 CHAPTER 3 : ASSESSING NUTRIENT MANAGEMENT SCENARIOS FOR THE CACHE LA POUDRE WATERSHED IN COLORADO . 28 3.1: Introduction . 28 3.2: Methods . 30 3.2.1: Overall Framework for Assessment of Water Quality Control Measures . 30
Morphotectonics of the Pescara River basin (Central Italy)

ABSTRACT This work presents a morphotectonic map of the Pescara River basin (1:40,000 scale), located in the piedmont-hilly area of the central-eastern Apennines (Abruzzo Region, Italy), and stretching from the eastern slope of the chain (Maiella Massif, Morrone Mt. and Gran Sasso Range) to the Adriatic coast. It is the outcome of an extensive drainage basin-scale geomorphological analysis and incorporates four main sections: orography and hydrography; morphotectonics; azimuthal analysis of the morphotectonic elements; Quaternary fluvial deposits and morphotectonic profiles. The morphotectonic map provides a basis for the study of morphotectonic features at basin-scale, the reconstruction of paleo-landscapes with special regard to drainage network evolution, and the recognition of tectonic control and selective erosion on the landscape. It also contributes to an outline of the main phases of the post-orogenic landscape evolution of the piedmont-hilly area of the Apennines, resulting from the link of alternating morphotectonic and surface processes.
Ultra‐high temperature metamorphism recorded in Fe‐rich olivine‐bearing migmatite from the Khondalite Belt, North China Craton

We document the first occurrence of Fe‐rich olivine‐bearing migmatitic metapelite in the Khondalite Belt, North China Craton. Petrological analyses revealed two exotic assemblages of orthopyroxene+spinel+olivine and orthopyroxene+spinel+cordierite. Phase relation modelling suggests that these assemblages are diagnostic of ultra‐high temperature (UHT) metamorphism in the Fe‐rich system, with temperatures from 1,000 to 1,050°C at 0.6 GPa. U–Th–Pb SIMS analyses on zircon reveal a similar age of c. 1.92 Ga for the olivine‐bearing migmatite and an adjacent gabbronoritic intrusion that is therefore identified as the heat source for the UHT metamorphism. These results, coupled with additional analysis of the famous Tuguiwula sapphirine‐bearing granulite, lead to a re‐appraisal of the P–T path shape and heat source for the UHT metamorphism. We suggest that UHT metamorphism, dated between 1.92 and 1.88 Ga, across the whole Khondalite belt, proceeded from a clockwise P–T evolution with an initial near‐isobaric heating path at ~0.6–0.8 GPa, and a maximum temperature of 1,050°C followed by a cooling path with minor decompression to ~0.5 GPa. Considering our results and previous works, we propose that the orogenic crust underwent partial melting at temperature reaching 850°C and depth of ~20 to ~30 km during a period of c. 30 Ma, between 1.93 and 1.90 Ga. During this time span, the partially molten crust was continuously intruded by mafic magma pulses responsible for local greater heat supply and UHT metamorphism above 1,000°C. We propose that the UHT metamorphism in the Khondalite belt is not related to an extensional post‐collisional event, but is rather syn‐orogenic and associated with mafic magma supplies.
Research on the Application and Mechanisms of Electroactive Microorganisms in Toxicants Monitoring: A Review

A biological treatment is the core process for removing organic pollutants from industrial wastewater. However, industrial wastewater often contains large amounts of toxic and harmful pollutants, which can inhibit the activity of microorganisms in a treatment system, precipitate the deterioration of effluent quality, and threaten water ecological security from time to time. In most of the existing anaerobic biological treatment processes, toxic effects on microorganisms are determined according to the amounts of end-products of the biochemical reactions, and the evaluation results are relatively lacking. When microorganisms contact toxic substances, changes in biological metabolic activity precede the accumulation of reaction products. As sensitive units, electroactive microorganisms can generate electrical signals, a change in which can directly reflect the toxicity level. The applications of electroactive microorganisms for the toxicity monitoring of wastewater are very promising. Further attention needs to be paid to considering the appropriate evaluation index, the influence of the environment on test results, mechanisms, and other aspects. Therefore, we reviewed the literature regarding the above aspects in order to provide a research foundation for the practical application of electroactive microorganisms in toxicant monitoring.
Access to the Health Care System of Undocumented Moroccan Migrant Slum Dwellers in Southern Spain: A Qualitative Study

Spain has grown economically due to the recent boosts in the industrial sector, the agricultural sector, construction and services. Those who carry out agricultural tasks are mainly undocumented immigrants living in marginal neighborhoods. Objectives: to know the perception of undocumented Moroccan migrants living in marginal neighborhoods regarding access to the Public Health System. Methods: A qualitative method with a phenomenological approach was used to get closer to the experience of the participants in the study. In this work, 24 semi-structured interviews were conducted following a guide with a set of open questions to facilitate an in-depth discussion of the topics of interest. The participants were given an informed consent form, which guaranteed anonymity and confidentiality regarding the information obtained. For this, codes were used to identify them. The data were stored, managed, classified, and organized with the ATLAS-ti 9 software. Results: access and use of health services by the immigrants in the study was difficult due to communication problems related to language and culture, the discriminatory attitude and lack of cultural competence of health personnel, and the location of the neighborhoods (which were marginal far from urban centers), as well as the lack of transportation to health centers. Conclusions: it is an ethical imperative of the Health System to offer greater attention to the population in situations of extreme vulnerability, implement intercultural mediators and train health professionals in cultural competence.
Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco

The plant microbiota is believed to be an accessory genome that extends plant functions, forming holobionts together with the host plant. Plant disease resistance, therefore, is inextricably linked with plant microbiota, which play important roles in plant growth and health. To explore the relationship between plant microbiota and disease resistance, we investigated the tobacco microbiome of two varieties with contrasting disease-resistance levels to bacterial wilt and black shank diseases. Comparative microbiome analysis indicated that the resistant variety assembled a distinct microbiota with higher network complexity and diversity. While Pseudomonas and Ensifer, which contain biocontrol and beneficial members, were enriched in the rhizosphere of the resistant variety, Ralstonia, a genus including the known causative pathogen, was enriched in the susceptible variety. Metagenome sequencing revealed that biocontrol functions, such as hydrogen cyanide synthase, pyochelin biosynthesis, and arthrofactin-type cyclic lipopeptide synthetase, were more abundant in the resistant variety. Further analysis indicated that contigs encoding the corresponding genes were mostly assigned to Pseudomonas. Among all the metagenome-assembled genomes, positive selection was suggested in the genome assigned to Pseudomonas only in the rhizosphere of the resistant variety. The search of biosynthetic gene clusters in the Pseudomonas genome revealed a non-ribosomal peptide synthetase, the compound of which was brabantamide A, with known antimicrobial activity. Collectively, our study suggests that the plant microbiota might be involved in microbe-mediated disease resistance. Particularly, our results highlight Pseudomonas in the rhizosphere of the disease-resistant variety as a promising biocontrol candidate. Our study may facilitate further screening of bacterial isolates and the targeted design of microbial communities.
Study of Structural Properties of Solar Energy Material CH3 NH3 PbX3 (X= I, Br and Cl) Perovskites and its Mixed Halides Using Density Functional Theory

The present study has explored the electronic properties of Organic-inorganic mixed halide-based perovskites using density functional theory. The Quantum Espresso simulation package with PBE parameterization within the generalized gradient approximation (GGA) has been used in this work. The findings reveal that the addition of halogens (bromide and chlorine) to compound CH3NH3PbI3 for all the possible substituted halides may cause the shifting of energy bands in the band structure and thus change the bandgap. Hence, this calculation may be used to design perovskite solar cell material with varied electronic structure.
Sugarcane production in Malawi : pest, pesticides and potential for biological control

Capacity Building for Climate Change Adaptation in Malawi (CABMACC) project number 1207026003. CABMACC is a collaborative project supported by the Norwegian Government and the Government of the Republic of Malawi implemented by International Environment and Development Studies (Noragric) of Norwegian University of Life Sciences (NMBU) and Lilongwe University of Agriculture and Natural Resources, (LUANAR), Malawi.