Miracle-dz/UniFAR-FTUs huggingface dataset:
Pyrin binds the PSTPIP1/CD2BP1 protein, defining familial Mediterranean fever and PAPA syndrome as disorders in the same pathway

Pyrin, the familial Mediterranean fever protein, is found in association with the cytoskeleton in myeloid/monocytic cells and modulates IL-1β processing, NF-κB activation, and apoptosis. These effects are mediated in part through cognate interactions with the adaptor protein ASC, which shares an N-terminal motif with pyrin. We sought additional upstream regulators of inflammation by using pyrin as the bait in yeast two-hybrid assays. We now show that proline serine threonine phosphatase-interacting protein [PSTPIP1, or CD2-binding protein 1 (CD2BP1)], a tyrosine-phosphorylated protein involved in cytoskeletal organization, also interacts with pyrin. Recently, PSTPIP1/CD2BP1 mutations were shown to cause the syndrome of pyogenic arthritis, pyoderma gangrenosum, and acne (PAPA), a dominantly inherited autoinflammatory disorder mediated predominantly by granulocytes. Endogenous PSTPIP1/CD2BP1 and pyrin are coexpressed in monocytes and granulocytes and can be coimmunoprecipitated from THP-1 cells. The B box segment of pyrin was necessary and the B box/coiled-coil segment sufficient for this interaction, whereas the SH3 and coiled-coil domains of PSTPIP1/CD2BP1 were both necessary, but neither was sufficient, for pyrin binding. The Y344F PSTPIP1/CD2BP1 mutation, which blocks tyrosine phosphorylation, was associated with a marked reduction in pyrin binding in pervanadate-treated cells. PAPA-associated A230T and E250Q PSTPIP1/CD2BP1 mutations markedly increased pyrin binding as assayed by immunoprecipitation and, relative to WT, these mutants were hyperphosphorylated when coexpressed with c-Abl kinase. Consistent with the hypothesis that these mutations exert a dominant-negative effect on the previously reported activity of pyrin, we found increased IL-1β production by peripheral blood leukocytes from a clinically active PAPA patient with the A230T PSTPIP1/CD2BP1 mutation and in cell lines transfected with both PAPA-associated mutants.
Economics for an Imperfect World: Essays in Honor of Joseph E. Stiglitz

Throughout Joseph Stiglitz's long and distinguished career in economics, the focus has been on the real world, with all of its imperfections. His 2001 Nobel Prize recognized his pioneering research in imperfect information; his work in other areas, including macroeconomics, public economics, and development economics, has been just as influential. This volume, a collection of essays written to mark Stiglitz's sixtieth birthday, reflects the wide-ranging influence of "Stiglitzian" economics. The many distinguished contributors are his teachers, students, and coauthors; their participation testifies to the personal and professional impact of Joseph Stiglitz's contributions to contemporary economic thought.
Utilização da internet nas atividades de monitoramento ambiental em empresas de pequeno e médio porte

This article aims to understand the importance of systematic environmental scanning in SMEs (small and medium enterprises), whereas the understanding of how companies seek and use information from the Internet is of great relevance to this process, keeping them more competitive in their market. For this was a literature review of environmental scanning, evaluating that several generic models are described in the literature, although many don’t direct their efforts for small and medium enterprises. The methodology used was a descriptive single case study to present the procedures performed by a company that uses data from the Internet in its environmental scanning, comparing the practice used with the steps of the model described in the literature. The results showed that the Internet is used as an important source for environmental scanning of this company, but the search for on-line information happens without systematic, intuitively and reactively. This article contributes to help SMEs to perceive the importance of scanning the environment and begin to adopt a formal model that systematizes the search and use of on-line information, making them more strategic to their business.
Entering the lysosome through a transient gate by chaperone-mediated autophagy

A subset of cytosolic proteins can be selectively degraded in lysosomes through chaperone-mediated autophagy. The lysosomal-membrane protein type 2A (LAMP-2A) acts as the receptor for the substrates of chaperone-mediated autophagy (CMA), which should undergo unfolding before crossing the lysosomal membrane and reaching the lumen for degradation. Translocation of substrates is assisted by chaperones on both sides of the membrane, but the actual steps involved in this process and the characteristics of the translocation complex were, for the most part, unknown. We have now found that rather than a stable translocon at the lysosomal membrane, CMA substrates bind to monomers of LAMP-2A driving the organization of this protein into a high molecular weight multimeric complex that mediates translocation. Assembly and disassembly of LAMP-2A into and from this complex is dynamic and it is regulated by hsc70 and hsp90, the two lysosomal chaperones related to CMA. This work thus unveils a unique mechanism of protein translocation across the lysosomal membrane, which involves only transient discontinuity of the membrane. The possible advantages of this transitory lysosomal translocon are discussed in light of the unique properties of the lysosomal compartment. Addendum to: Bandyopadhyay U, Kaushik S, Vartikovski L, Cuervo AM. Dynamic organization of the receptor for chaperone-mediated autophagy at the lysosomal membrane. Mol Cell Biol 2008; 28:5747-63; DOI: 10.1128/MCB.02070-07.
Circular Microstrip Patch Loaded With Balanced Shorting Pins for Improved Bandwidth

A shorted patch geometry is examined with a circular patch loaded with a pair of balanced shorting pins. The proposed design is simple and straight forward as the location of the balanced pins are fixed with respect to the patch center. Nearly double the impedance bandwidth has been achieved with a balanced pin loaded circular patch operating in X band without sacrificing the gain or radiation properties compared to its conventional counterpart. This is an important advantage of the proposed configuration. Both measured and simulation results are presented.
Contracts, constraints, and consumption

The paper compares implications of three kinds of models of households’ consumption behavior: the basic permanent-income model, several models of liquidity-constrained households, and a model of an informationally-constrained efficient contract. These models are distinguished in terms of implications regarding the present discounted values of net trades to households at various levels of temporary income, and the households’ marginal rates of substitution. Martingale consumption is studied as an approximation to the predicted consumption process of the efficient-contract model.
A Filarial Nematode-Secreted Product Signals Dendritic Cells to Acquire a Phenotype That Drives Development of Th2 Cells1

Although exogeneous “danger” signals such as LPS can activate APC to produce a Th1 response, the nature of events initiating a Th2 response is controversial. We now show that pathogen-derived products have the capacity to induce bone marrow-derived dendritic cell cultures to acquire a phenotype that promotes the differentiation of naive CD4+ T cells toward either a Th1 or Th2 phenotype. Thus, LPS-matured dendritic cells (DC1) promote a Th1 response (increased generation of IFN-γ and reduced production of IL-4) by Ag-stimulated CD4+ T cells from the DO.11. 10 transgenic mouse expressing a TCR specific for an OVA peptide (OVA323–339). In contrast, a phosphorylcholine-containing glycoprotein, ES-62, secreted by the filarial nematode, Acanthocheilonema viteae, which generates a Th2 Ab response in vivo, is found to induce the maturation of dendritic cells (DC2) with the capacity to induce Th2 responses (increased IL-4 and decreased IFN-γ). In addition, we show that the switch to either Th1 or Th2 responses is not effected by differential regulation through CD80 or CD86 and that a Th2 response is achieved in the presence of IL-12.
Maintaining economic stability as a motive for statutory accounting requirements

The impact of selected accounting rules on economic stability is analysed. Maintaining economic stability is claimed to be an important motive for regulatory intervention. In the first part of the paper, a framework for analysing the usefulness of such stabilizing intervention is proposed. There are differences between countries relating to the kind of economic relationship regulators are focusing on. To what extent should standard setting activities attempt to stabilize relationships between debtors and creditors rather than between capital market players? Should the determination of distributable profits or investor information be the predominating tool of policy making? In relation to these issues several common accounting rules are examined for their contribution to economic stability.
Smoke, mirrors and poverty: communication, biotechnologicalinnovation and development

Communication is essential to making biotechnology and genomics relevant to developing countries and poor people. Few would disagree with that. But many are sceptical about public relations efforts to impose inappropriate technological ‘solutions’ on developing countries. This paper is a partial reflection on how PR and advocacy ‘mixes’ can be understood and whether they can be useful to innovation in developing country contexts. This paper has several aims: First to consider why communication has become more important in the area of innovation and development; Second, we look at how two biotechnology related public-private partnerships have used public relations and advocacy to further innovation in development and pose some questions about complicated aspects of communication, technological innovation and development. We suggest that it is increasingly difficult to classify communication efforts associated with technology for development initiatives as PR or advocacy or according to the preconceived notions about who the messenger might be; Third we look at some of the methodological and theoretical implications of the analysis. Discourse analysis, which encourages us to unwrap layers of meaning in the text but which often treats texts in the abstract, unrelated to broader institutional developments or to ‘evidence’ of any kind, is of limited help in achieving a more grounded analysis of communication efforts. Communication and voice are essential ‘capabilities’ in development and we suggest that we need a more sophisticated approach to thinking about communication capabilities as technical, and social and political.
Deficits in theory of mind and Executive function in children with Attention Deficit Hyperactivity Disorder

This study was designed to examine the existence of deficits theory of mind (ToM) and Executive function(EF) in children with Attention Deficit Hyperactivity Disorder (ADHD). Tom and EF functioning was assessed in 40 children aged 6-8 years with (ADHD( and normal children. The Sally– Anne tes(Wimmer & Perner, 1983) t is a psychological test, used in developmental psychology to measure TOM. The Behavior Rating Inventory of Executive Function (BRIEF) was developed by Gioia et al. (1996) measures of EF. DSM-IV used for assessing ADHD. The group(ADHD(was significantly impaired relative to controls on the advanced ToM measure and EF. (ADHD( may disrupt the developmental acquisition of emotion recognition and advanced ToM skills. The clinical and theoretical importance of these findings is discussed and the implications for the assessment and treatment of children who have experienced ADHD are outlined.
Voltage Balancing Control of Improved ZVS IFBTL Converter for WECS RAMADUGU SITARA

This project proposes a newly developed three level dc-dc converter which is designed with zero voltage switching technique for the purpose of reducing conduction loss and frequent switching of the switches used in the converter. A passive filter is used to reduce the primary voltage stress of medium frequency transformer and to improve the performance of the converter. A modulation strategy, including two operation modes, is proposed for the IFBTL dc/dc converter. Furthermore a voltage balancing control strategy is also proposed for the improved ZVS full bridge three level DC-DC converter. With the passive filter and the modulation strategy, the voltage stress of the transformer in the Improved ZVS FBTL DC/DC converter can be effectively reduced, which is very significant in the mediumvoltage and high-power application. It is performed by designing a full bridge three level converter with ZVS technique and a passive filter and the converter is simulated using MATLAB SIMULINK.
Transformation of Immigrant Integration in Western Europe: Civic Integration and Antidiscrimination Policies in the Netherlands, France, and Germany

This article argues that, beginning in the mid-1990s, there has been a transformation of immigrant integration policies in Western Europe, away from distinct "national models" and toward convergent policies of "civic integration" for newcomers and "antidiscrimination" for settled immigrants and their descendants. This convergence is demonstrated by a least-likely case comparison of the Netherlands, France, and Germany—states that had pursued sharply different lines in the past. The author fleshes out the conflicting, even contradictory logics of antidiscrimination and civic integration and grounds them in opposite variants of liberalism, an "old" liberalism of nondiscrimination and equal opportunity and a "new" liberalism of power and disciplining, respectively.
Prophylactic deoxyspergualin treatment in living-related renal-transplant recipients transfused with donor-specific blood

Background. Deoxyspergualin (DSG) prophylaxis has improved long-term graft survival in living-related renal-transplant recipients transfused with donor-specific blood (DST). We examined the influence of acute rejection (AR) on graft survival in these patients. Methods. The study groups consisted of either historic control recipients without DSG (group A, n=64, 1985–1989) and recipients with DSG as the initial immunosuppressive agent (group B, n=76, 1989–1995). Both groups received DST from a one-haplotype identical donor and were treated with cyclosporine-based immunosuppression. Rejection was classified into accelerated rejection (Acc, within 5 days), AR (from 6 days–3 months), and late AR (LAR, from 4 months–1 year). Results. Overall 5-year graft survival rates were significantly higher in group B than group A (89.5 vs. 73.4%, P =0.0070). Each group was then subdivided on the basis of whether or not they had an episode of Acc, AR, or LAR. In group A, 5-year graft survival rate was not affected the presence or absence of Acc (75.0 vs. 73.1%), and it was influenced significantly by the presence or absence of AR (50.0 vs. 85.7%, P =0.0012) or LAR (46.7 vs. 81.6%, P <0.0001). In group B, 5-year graft survival did not change significantly by the presence or absence of Acc (100 vs. 88.7%), AR (81.8 vs. 92.6%), or LAR (81.0 vs. 92.7%). Conclusions. Prophylactic use of DSG in living-related renal-transplant recipients treated with DST improves long-term graft survival, even in patients with AR episodes.
MONETARY REGIMES AND ENDOGENOUS WAGE CONTRACTS: SWEDEN 1908-1995

We examine Gray´s theory of endogenous length of wage contracts and inflation indexation, using a uniquely long data set of blue-collar worker collective agreements in Sweden 1908-1995. Volatile monetary regimes, i. e. regimes with large macroeconomic uncertainty, are associated with short length and inflation indexed contracts. We also find inertia in changes in contract characteristics to changes in the regime. The findings support the Lucas critique, but cautions against simple divisions of time series in different regimes wherein agents' decision rules are assumed constant.
Affine Invariant Comparison of Molecular Shapes with Properties

We present an algorithm of matching 3D shapes in an affineinvariant way using multi-resolution dual contour trees en hanced by geometrical, topological, and functional properties. D ual contour trees (DCT) are simplified structures generated from co ntour trees of volumetric functions. Each node of a DCT correspond s to a set of connected arc segments of the original contour tree, which is a connected sub-volume bounded by contours. DCT nodes are enhanced with geometrical, topological, and functional at tributes. Similarities among 3D molecular shapes are computed by matc hing nodes from multi-resolution DCT’s and their attributes . This algorithm shows good performance in clustering proteins in to different families compared to standard inner-product method s.
Three Steps Forward, Two Steps Backward: The Status of Women's Integration into Public Management

long way to go before they will reach parity. T oday, as in the past, women must convince men that their demands for governmental action are legitimate before their desires are transformed into policies, programs, and services. This is because men hold the vast majority of decision-making positions in government. In legislative chambers and top-level career posts, women's voices are still mostly heard indirectly through the mouths of their husbands, fathers, brothers, and sons. When Abigail Adams reminded her husband to "remember the ladies" as a new nation was being founded, he responded in jest to her. The thought of treating women as full-fledged citizens was considered frivolous. Two hundred years later, women's political status has improved, but women remain largely dependent on the goodwill of the men around them. This is true in policy implementation and program management as well as in the political arena.
The internationalisation of small- and medium-sized management consultancies: an exploratory study of key facilitating factors

This paper presents preliminary case-study evidence on key factors associated with the internationalisation of management consultancy small- to medium-sized enterprises (SMEs), including how these factors differ from previous aggregate evidence based mainly on manufacturing firms. It contributes by adding to the limited empirical literature on the internationalisation of service firms. The analysis results suggest the salience of four explored factors, including owner/founder's international orientation, networks and relationships, niche focus, and technology leveraging. Among the implications discussed is the need for managers of service-sector SMEs to ensure an abundance of internationalisation-friendly mindsets at the apex of their businesses. Future researchers on the internationalisation of management consultancy SMEs are urged to build on this exploratory effort using a quantitative research design approach.
PbSe Nanocrystal Solids for n- and p-Channel Thin Film Field-Effect Transistors

Initially poorly conducting PbSe nanocrystal solids (quantum dot arrays or superlattices) can be chemically “activated” to fabricate n- and p-channel field effect transistors with electron and hole mobilities of 0.9 and 0.2 square centimeters per volt-second, respectively; with current modulations of about 103 to 104; and with current density approaching 3 × 104 amperes per square centimeter. Chemical treatments engineer the interparticle spacing, electronic coupling, and doping while passivating electronic traps. These nanocrystal field-effect transistors allow reversible switching between n- and p-transport, providing options for complementary metal oxide semiconductor circuits and enabling a range of low-cost, large-area electronic, optoelectronic, thermoelectric, and sensing applications.
Physician assistants and nurse practitioners in the National Health Service Corps

ABSTRACTObjectives: This study describes the experiences of physician assistants (PAs) and nurse practitioners (NPs) in the National Health Service Corps' (NHSC) loan repayment program in 2010.Methods: In 2011, a stratified random sample of NHSC clinicians was surveyed. Data from the 148 PA and 137 NP respondents were analyzed (52.4% response rate). Results: PAs were younger than NPs (mean age 31 versus 35 years), less often female (68% versus 91%), and more often carried educational debt over $100,000 (56% versus 24%). Both groups were serving in states familiar to them and within communities where they felt accepted. The groups were generally satisfied on most measures of work, with PAs more satisfied than NPs on some measures.Conclusion: The NHSC's PAs and NPs are well matched to communities and satisfied with their work. Maximizing their NHSC experiences and retention requires recognizing their differences in demographics, debt, and areas of job satisfaction.
Mutational Study on the Roles of Disulfide Bonds in the β-Subunit of Gastric H+,K+-ATPase*

The gastric proton pump, H+,K+-ATPase, consists of the catalytic α-subunit and the non-catalytic β-subunit. Correct assembly between the α- and β-subunits is essential for the functional expression of H+,K+-ATPase. The β-subunit contains nine conserved cysteine residues; two are in the cytoplasmic domain, one in the transmembrane domain, and six in the ectodomain. The six cysteine residues in the ectodomain form three disulfide bonds. In this study, we replaced each of the cysteine residues of the β-subunit with serine individually and in several combinations. The mutant β-subunits were co-expressed with the α-subunit in human embryonic kidney 293 cells, and the role of each cysteine residue or disulfide bond in the α/β assembly, stability, and cell surface delivery of the α- and β-subunits and H+,K+-ATPase activity was studied. Mutant β-subunits with a replacement of the cytoplasmic and transmembrane cysteines preserved H+,K+-ATPase activity. All the mutant β-subunits with replacement(s) of the extracellular cysteines did not assemble with the α-subunit, resulting in loss of H+,K+-ATPase activity. These mutants did not permit delivery of the α-subunit to the cell surface. Therefore, each of these disulfide bonds of the β-subunit is essential for assembly with the α-subunit and expression of H+,K+-ATPase activity as well as for cell surface delivery of the α-subunit.
Social Housing Managers and the Performance Ethos

In sociology, ‘the professional project’ is understood as a collective endeavour of occupational groups that only succeeds if those groups possess, and control access to, a unique stock of knowledge. Urban sociologists have been critical of public housing managers’ collective endeavours to present themselves as a profession because they use generic knowledge and common sense in their work. They also argue that ‘the professional project’ of housing management is being further under-mined by the ‘performance ethos’, since this now allows service managers to exert even more control over what public housing managers do and thus de-skills them even more. Our argument is that this analysis of the impact of the performance ethos is based on a conceptually limiting view of power as a repressive force that enforces ‘blanket restrictions’ on group activity, i.e. what professional groups are free to do. Conversely, we adopt a Foucauldian view of power because it better explains our research findings. Foucault suggests that power does not simply repress group activity. Power is also appropriated by individuals who use it to redefine themselves, e.g. who and what they are. We draw on our empirical data to show how individual housing managers were appropriating the performance culture in productive ways to achieve their own individual ends (i.e. to ‘work on’ their professional selves so as to re-define themselves and thus their individual claim to professional status). We use this analysis to argue that an individualized (as opposed to collective) ‘professional project of the self’ is emerging in housing management that has not yet been adequately captured in the sociological literature.