Miracle-dz/UniFAR-FTUs huggingface dataset:
Gephyrin Is Critical for Glycine Receptor Clustering But Not for the Formation of Functional GABAergic Synapses in Hippocampal Neurons

The role of the scaffolding protein gephyrin at hippocampal inhibitory synapses is not well understood. A previous study (Kneussel et al., 1999) reported a complete loss of synaptic clusters of the major GABAAR subunits α2 and γ2 in hippocampal neurons lacking gephyrin. In contrast, we show here that GABAAR α2 and γ2 subunits do cluster at pyramidal synapses in hippocampal cultures from gephyrin-/- mice, albeit at reduced levels compared with control neurons. Synaptic aggregation of GABAAR α1 on interneurons was identical between the culture types. Furthermore, we recorded miniature IPSCs (mIPSCs) from gephyrin-/- neurons. Although the mean mIPSC amplitude was reduced (by 23%) compared with control, the frequency of these events was unchanged. Cell surface labeling experiments indicated that gephyrin contributes, in part, to aggregation but not to insertion or stabilization of GABAAR α2 and γ2 in the plasma membrane. Thus, a major gephyrin-independent component of hippocampal inhibitory synapse development must exist. We also report that glycine receptors cluster at GABAergic synapses in a subset of hippocampal interneurons and pyramidal neurons. Unlike GABAARs, synaptic clustering of glycine receptors was completely abolished in gephyrin-/- neurons. Finally, artificial extrasynaptic aggregation of GABAAR was able to redistribute and cocluster gephyrin by a mechanism requiring a neuron-specific modification or intermediary protein. We propose a model of hippocampal inhibitory synapse development in which some GABAARs cluster at synapses by a gephyrin-independent mechanism and recruit gephyrin. This clustered gephyrin may then recruit glycine receptors, additional GABAARs, and other signal-transducing components.
How To Evaluate Lessons from the Past with Illustrations from the Case of Pearl Harbor.

Policy makers use past experience and history to think about current and potential problems and to explain policies and problems to others. Decision makers may be overly influenced by significant personally-experienced events that loom so large in their eyes that details and related relevant information may pale in comparison. Deficiencies in content or logic may ultimately not matter in the quality or political efficacy of a lesson, but need to be assessed along with the context and effect of the lesson to establish a complete assessment of that lesson and the role the lesson played in the decision making process. Learning can be said to have taken place with regard to examination of a historically based lesson if that experience leads to change or altered policies. This requires that a baseline be established on what relevant policy makers believed about the world prior to the event. Decision makers do not use history all that poorly when the political context and the actual content of lessons are taken into account. Decision makers do pay attention to current context and that context, not the past, heavily influences the uses of the past. The evidence for the ways to look at lessons from the past are largely drawn from the case of Pearl Harbor and its influence on U.S. post-war defense policy. A list of 28 references is included. (PPB) ***** *** ***** ** ******* ********* ********* ***** ***** *** ***** ************* Reproductions supplied by EDRS are the best that can be made from the original document. ***** * ********* * ****** ****** ***** ****** ***** * ***** **** ******* ***** ***** How to Evaluate Lessons From the Past with Illustrations from the Case of Pearl Harbor
Deleterious Associations of Sitting Time and Television Viewing Time With Cardiometabolic Risk Biomarkers

OBJECTIVE We examined the associations of sitting time and television (TV) viewing time with continuously measured biomarkers of cardio-metabolic risk in Australian adults. RESEARCH DESIGN AND METHODS Waist circumference, BMI, resting blood pressure, triglycerides, HDL cholesterol, fasting and 2-h postload plasma glucose, and fasting insulin were measured in 2,761 women and 2,103 men aged ≥30 years (mean age 54 years) without clinically diagnosed diabetes from the 2004–2005 Australian Diabetes, Obesity and Lifestyle (AusDiab) study. Multivariate linear regression analyses examined associations of self-reported sitting time and TV viewing time (hours per day) with these biomarkers, adjusting for potential confounding variables. RESULTS For both women and men, sitting time was detrimentally associated with waist circumference, BMI, systolic blood pressure, fasting triglycerides, HDL cholesterol, 2-h postload plasma glucose, and fasting insulin (all P < 0.05), but not with fasting plasma glucose and diastolic blood pressure (men only). With the exception of HDL cholesterol and systolic blood pressure in women, the associations remained significant after further adjustment for waist circumference. TV viewing time was detrimentally associated with all metabolic measures in women and all except HDL cholesterol and blood pressure in men. Only fasting insulin and glucose (men only) remained deleteriously associated with TV viewing time after adjustment for waist circumference. CONCLUSIONS In women and men, sitting time and TV viewing time were deleteriously associated with cardio-metabolic risk biomarkers, with sitting time having more consistent associations in both sexes and being independent of central adiposity. Preventive initiatives aimed at reducing sitting time should focus on both nonleisure and leisure-time domains.
Rescue from Light-Induced Retinal Degeneration by Human Fetal Retinal Transplantation in Minipigs

Purpose: To evaluate changes in retinal morphology and visual function after transplantation of human fetal neuroretina and retinal pigment epithelium (RPE) in a minipig model of light-induced retinal degeneration. Methods: Photoreceptor degeneration was induced by 2500 lux white light exposure for six months in 16 minipigs. Human fetal (12–24 week) neuroretina and RPE were transplanted into the subretinal space adjacent to the central retina in 25 eyes. Sham operation was performed in three eyes. The operated eyes were examined by multifocal electroretinogram (mfERG), fundus fluorescence angiography (FFA), and histology for up to 12 months. Results: Subretinal transplantation of neuroretina and RPE was successful in 15 out of 25 eyes (60%), among which 10/15 eyes showed evidence of the grafted tissue in subsequent histology. Structural processes between the graft and host tissue were observed from one month post implantation, accompanied by increased numbers of GFAP-positive cells over time. Immunohistochemistry showed the presence of GFAP- and Chx10-positive cells, but rhodopsin staining was not observed within the grafted tissue. In 15 eyes, mfERG revealed retinal functional improvement in regions both inside and outside of the grafted area, but this was not observed in sham-operated eyes. FFA showed no vascular leakage or inflammatory cells in eyes receiving tissue transplants. Conclusions: Following subretinal transplantation, the grafted fetal neuroretina and RPE can survive for up to 12 months without signs of graft rejection, and the host retinas showed functional improvement over the same period. Our data suggest that subretinal transplantation of neuronal retina and RPE might be beneficial in improving retinal function in cases of retinal degeneration.
Methods for Indexing Stripes in Uncoded Structured Light Scanning Systems

This paper presents robust methods for determining the order of a sequence of stripes captured in an uncoded structured light scanning system, i.e. where all the stripes are projected with uniform colour, width and spacing. A single bitmap image shows a pattern of vertical stripes from a projected source, which are deformed by the surface of the target object. If a correspondence can be determined between the projected stripes and those captured in the bitmap, a spatial measurement of the surface can be derived using standard rangefinding methods. Previous work has uniquely encoded each stripe, such as by colour or width, in order to avoid ambiguous stripe identification. However, colour coding suffers due to uneven colour reflection, and a variable width code reduces the measured resolution. To avoid these problems, we simplify the projection as a uniform stripe pattern, and devise novel methods for correctly indexing the stripes, including a new common inclination constraint and occlusion classification. We give definitions of patches and the continuity of stripes, and measure the success of these methods. Thus we eliminate the need for coding, and reduce the accuracy required of the projected pattern; and, by dealing with stripe continuity and occlusions in a new manner, provide general methods which have relevance to many structured light problems.
Anti-Inflammatory Activity of Delonix regia (Boj. Ex. Hook)

The present work was to evaluate the anti-inflammatory activity of Delonix regia leaves (Family: Caesalpiniaceae). The powder of Delonix regia leaves was subjected to extraction with ethanol in soxhlet extractor. The ethanol extract after preliminary phytochemical investigation showed the presence of sterols, triterpenoids, phenolic compounds and flavonoids. The anti-inflammatory activity was studied using carrageenan-induced rat paw edema and cotton pellet granuloma at a three different doses (100, 200, and 400 mg/kg b.w. p.o.) of ethanol extract. The ethanol extract of Delonix regia leaves was exhibited significant anti-inflammatory activity at the dose of 400 mg/kg in both models when compared with control group. Indomethacin (10 mg/kg b.w. p.o) was also shown significant anti-inflammatory activity in both models.
Smooth invariant interpolation of rotations

We present an algorithm for generating a twice-differentiable curve on the rotation group SO(3) that interpolated a given ordered set of rotation matrices at their specified knot times. In our approach we regard SO(3) as a Lie group with a bi-invariant Riemannian metriac, and apply the coordinate-invariant methods of Riemannian geometry. The resulting rotation curve is easy to compute, invariant with respect to fixed and moving reference frames, and also approximately minimizes angular acceleration.
Requirement for Capsule in Colonization byStreptococcus pneumoniae

ABSTRACT Nasopharyngeal colonization is a necessary first step in the pathogenesis of Streptococcus pneumoniae. Using isolates containing defined mutations in the S. pneumoniaecapsule locus, we found that expression of the capsular polysaccharide is essential for colonization by the type 2 strain D39 and the type 3 strains A66 and WU2. Nonencapsulated derivatives of each of these strains were unable to colonize BALB/cByJ mice. Similarly, type 3 mutants that produced <6% of the parental amounts of capsule could not colonize. Capsule production equivalent to that of the parent strain was not required for efficient colonization, however, as type 3 mutants producing approximately 20% of the parental amounts of capsule colonized as effectively as the parent. This 80% reduction in capsule level had only a minimal effect on intraperitoneal virulence but caused a significant reduction in virulence via the intravenous route. In the X-linked immunodeficient CBA/N mouse, the type 3 mutant producing ∼20% of the parental amount of capsule (AM188) was diminished in its ability to cause invasive disease and death following intranasal inoculation. Following intravenous or intraperitoneal challenge, however, only extended survival times were observed. Our results demonstrate an additional role for capsule in the pathogenesis ofS. pneumoniae and show that isolates producing reduced levels of capsule can remain highly virulent.
SHORT‐ VERSUS LONG‐TERM RESPONSES TO CHANGING CO2 IN A COASTAL DINOFLAGELLATE BLOOM: IMPLICATIONS FOR INTERSPECIFIC COMPETITIVE INTERACTIONS AND COMMUNITY STRUCTURE

Increasing pCO2 (partial pressure of CO2) in an “acidified” ocean will affect phytoplankton community structure, but manipulation experiments with assemblages briefly acclimated to simulated future conditions may not accurately predict the long‐term evolutionary shifts that could affect inter‐specific competitive success. We assessed community structure changes in a natural mixed dinoflagellate bloom incubated at three pCO2 levels (230, 433, and 765 ppm) in a short‐term experiment (2 weeks). The four dominant species were then isolated from each treatment into clonal cultures, and maintained at all three pCO2 levels for approximately 1 year. Periodically (4, 8, and 12 months), these pCO2‐conditioned clones were recombined into artificial communities, and allowed to compete at their conditioning pCO2 level or at higher and lower levels. The dominant species in these artificial communities of CO2‐conditioned clones differed from those in the original short‐term experiment, but individual species relative abundance trends across pCO2 treatments were often similar. Specific growth rates showed no strong evidence for fitness increases attributable to conditioning pCO2 level. Although pCO2 significantly structured our experimental communities, conditioning time and biotic interactions like mixotrophy also had major roles in determining competitive outcomes. New methods of carrying out extended mixed species experiments are needed to accurately predict future long‐term phytoplankton community responses to changing pCO2.
Hierarchical Spatiotemporal Dynamics of Speech Rhythm and Articulation

Hierarchical spatiotemporal dynamics of speech rhythm and articulation by Samuel Edward Tilsen B.A. (Northwestern University) 2001 M.A. (University of California, Berkeley) 2005 A dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Linguistics in the Graduate Division of the University of California, Berkeley Committee in charge: Professor Keith Johnson, Chair Professor Sharon Inkelas Professor Richard Ivry Spring 2009
Mission reachability for extraterrestrial rovers

A methodology for the mobility evaluation and mission oriented performance assessment of an autonomous mobile robot is developed. The evaluation strategy uses both time and energy based measures resulting in the development of isochronal/isoenergy contours which characterize the expected mobility of the robot over novel terrain using sample trials on patches of land prior to the mission. This work has a basis in the evaluation of a robot for extraterrestrial exploration and demonstrates the feasibility of the evaluation methodology proposed using simulations. Simulation results are reported for two designs of micro-rovers viz. legged and wheeled on a simulated Martian terrain. Future work is discussed in the context of increased complexity due to hardware implementations, better terrain models and statistical simulations all of which are currently under development.
Observed timescales of evapotranspiration response to soil moisture

The sensitivity of evapotranspiration (ET) to soil moisture storage plays an important role in the land‐atmosphere system. Yet little is known about its magnitude, or its dependence on vegetation, soil, and/or climate characteristics. Here we relate the sensitivity to the timescale of ET decay in absence of rainfall, and show that it can thus be derived from time series of ET alone. We analyze ET observations from 15 vegetated sites covering a range of climates conditions, yielding timescales of 15–35 days. Longer timescales (weaker ET sensitivity) are found in regions with seasonal droughts, or at sites with woody vegetation. We compare observed values with output of different land surface models (LSMs) from the Second Global Soil Wetness Project, revealing large inter‐model differences and significant differences with observations. Our methodology can lead to improved representation of soil moisture effects on ET in LSMs.
A joint optimization of incrementality and revenue to satisfy both advertiser and publisher

A long-standing goal in advertising is to reduce wasted costs due to advertising to people who are unlikely to buy, as well as to those who would make a purchase whether they saw an ad or not. The ideal audience for the advertiser are those incremental users who would buy if shown an ad, and would not buy, if not shown the ad. On the other hand, for publishers who are paid when the user clicks or buys, revenue may be maximized by showing ads to those users who are most likely to click or purchase. We show analytically and empirically that an optimization towards one metric might result in an inferior performance in the other one. We present a novel algorithm, called SLC, that performs a joint optimization towards both advertisers' and publishers' goals and provides superior results in both.
Human periodontal ligament cells secrete macrophage colony-stimulating factor in response to tumor necrosis factor-alpha in vitro.

BACKGROUND Human periodontal ligament (HPDL) cells may support osteoclastogenesis by expressing receptor activator of nuclear factor-kappa B ligand (RANKL) in response to periopathogenic factors and inflammatory cytokines. Because osteoclastogenesis requires the presence of macrophage colony-stimulating factor (M-CSF), we examined whether HPDL cells secrete M-CSF in response to tumor necrosis factor-alpha (TNF-alpha). METHODS Cultured HPDL cells were treated with TNF-alpha in serum-free condition. The expression of M-CSF and RANKL was determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Inhibitors and anti-TNF receptor (TNFR) neutralizing antibodies were used for the inhibitory experiments. A migration assay was performed. RESULTS TNF-alpha upregulated M-CSF and RANKL in HPDL cells. The effect on M-CSF expression could be partially blocked by pyrrolidine-dithiocarbamate ammonium salt and LY294002 but not by NS398. Neutralizing antibody to TNFR1 could diminish the effect of TNF-alpha. In addition, TNF-treated culture medium exhibited chemotactic effect for RAW264.7. CONCLUSIONS HPDL cells are capable of secreting M-CSF and expressing RANKL in response to TNF-alpha. The upregulation of M-CSF is possibly one of the mechanisms essential for periodontal tissue destruction in response to inflammatory cytokines. The upregulation is partly through nuclear factor-kappa B (NF-kappaB) and phosphatidylinositol 3'-kinase and possibly involves TNFR1.
Harmonic interaction between a large number of distributed power inverters and the distribution network

Power quality problems associated with distributed power (DP) inverters, implemented in large numbers onto the same distribution network, are investigated. Currently, these power quality problems are mainly found in projects with large penetration of photovoltaics (PV) on rooftops of houses and commercial buildings. The main object of this paper is to analyze the observed phenomena of harmonic interference of large populations of these inverters and to compare the network interaction of different inverter topologies and control options. These power quality phenomenons are investigated by using extensive laboratory experiments, as well as computer modeling of different inverter topologies. A complete network simulation study on an existing residential network with large penetration of PVs, is included.
Symptoms and related functioning in a traumatized community.

BACKGROUND Traumatic events are described as precipitating factors for medically unexplained symptoms. The aim of this study was to examine the prevalence and course of symptoms reported by disaster survivors and to assess whether the symptoms have features similar to those of medically unexplained symptoms. METHODS A 3-wave longitudinal study was performed after an explosion of a fireworks depot. As a result of the explosion, 23 people were killed, more than 900 people were injured, and about 500 homes were damaged or destroyed. Respondents completed a set of validated questionnaires measuring their health problems 3 weeks (wave 1), 18 months (wave 2), and 4 years (wave 3) after the disaster. A comparison group was included at waves 2 and 3. RESULTS The study population included 815 survivors who participated in the 3 waves. The mean number of symptoms was higher among survivors compared with control subjects at wave 2 (7.5 vs 5.8 symptoms) and at wave 3 (6.1 vs 4.9 symptoms) (P < .001 for both). Survivors and control subjects with more symptoms reported significantly lower mean scores on all scales of the Dutch version of the RAND 36-item health survey. Illness behavior and depression and anxiety were associated with the number of symptoms. For example, more than 60% of survivors with 10 or more symptoms reported depression and anxiety, compared with 2.4% of survivors with 0 to 1 symptoms (P < .001). CONCLUSIONS Up to 4 years after the disaster, symptoms were more prevalent among survivors than controls. Although medical disorders cannot be excluded, the reported symptoms showed several features similar to those of medically unexplained symptoms in the general population.
Atrioventricular node reentry with 'smooth' AV node function curves: a different arrhythmia substrate?

BACKGROUND Some patients with otherwise typical AV node reentry do not manifest discontinuous AV node function curves. We examined the effects of an ablation in the slow-pathway region in patients with smooth AV node function curves. METHODS AND RESULTS Fifteen patients with AV node reentrant tachycardia (AVNRT) and discontinuous AV node function curves were compared with 15 patients with AVNRT and smooth AV node function curves. In the group with discontinuous curve, the "net" anterograde effective refractory period (AERP) of the AV node increased (270 +/- 28 versus 304 +/- 37 ms, P = .03) and AERP of the remaining fast pathway decreased (367 +/- 100 versus 304 +/- 37 ms, P = .026) after the ablation. In the group with a smooth curve, the AERP of the AV node increased (266 +/- 42 versus 299 +/- 76 ms, P = .07) and the anterograde Wenckebach cycle length increased (336 +/- 66 versus 379 +/- 86 ms, P = .008) after the ablation. Retrograde conduction over the AV node was similar in both groups and was unchanged after ablation. The longest attainable AH interval (AHmax) measured during atrial extrastimulus testing was more prolonged in patients with a discontinuous curve than in patients with a smooth curve (326 +/- 48 versus 250 +/- 70 ms, P = .002). The AHmax shortened in both groups after ablation (326 +/- 48 versus 173 +/- 34 ms, P < .0001, and 250 +/- 70 versus 179 +/ 34 ms, P < .0003, respectively) and were similar. Successful ablation in the slow-pathway zone in patients with a smooth AV node function curve resulted in the loss of the "tail" of the curve representing the slow pathway. CONCLUSIONS These data suggest that the smooth AV node function curve consists of two distinct components representing both fast and slow AV node pathways even when the typical discontinuity is absent.
Using the Parsec environment to implement a high-performance processor farm

Parsec is a parallel programming environment whose goal is to simplify the development of multicomputer programs without, as is often the case, sacrificing performance. We have reconciled these objectives by "compiling" the structure of parallel applications into information to configure each of a small set of communication primitives on a context-sensitive basis. In this paper, we show how Parsec can be used to implement a high-performance processor farm and compare Parsec and hand-optimized implementations to demonstrate that Parsec can achieve a similar level of performance. Extensive static analysis and optimization is necessary to achieve these results. We discuss both the tools which perform these tasks as well as the user interface that provides the necessary declarative structural information. Using the processor farm, we show how Parsec simplifies the task of specifying the structure of a parallel application and improves the result by supporting abstraction, reuse and scalability. <<ETX>>