Miracle-dz/UniFAR-FTUs huggingface dataset:
Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract

Abstract In the current study, we used high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC–MS) to investigate and analyze the methanolic extract of Salvia rosmarinus leaves. HPLC analysis showed that the extract revealed a diverse array of polyphenolic compounds, including apigenin, catechin, chlorogenic acid, cinnamic acid, caffeic acid, coumaric acid, daidzein, ellagic acid, ferulic acid, gallic acid, hesperetin, kaempferol, methyl gallate, naringenin, pyrocatechol, quercetin, rutin, syringic acid, and vanillin. Furthermore, three fungal isolates from symptomatic strawberry plants were obtained and identified as Botrytis cinerea (OR116486), Fusarium oxysporum (OR116505), and Rhizoctonia solani (OR116525). The extract’s antifungal activity was evaluated at concentrations of 0, 50, 150, 200, and 300 µg/mL. At 200 µg/mL, the extract showed growth inhibition percentages of 74.56, 58.19, and 56.67% for R. solani, F. oxysporum, and B. cinerea, respectively, while at 300 µg/mL, all the tested fungi were completely suppressed. The GC–MS analysis revealed that the major compounds of the methanolic extract identified based on their retention times and relative peak areas (%) included β-caryophyllene (12.06%), germacrene d (13.55%), caryophyllene oxide (3.13%), methyl palmitate (5.26%), hexadecanoic acid (4.9%), and methyl stearate (6.02%). These results show rosemary extract’s potential as a source of natural antifungal agents against plant photogenic fungi. As a result, it provides a safer alternative to the current protective approaches for plant disease management.
Tectonic evolution of the Northern Salinian Block, California, USA: Paleogene to Recent shortening in a transform fault-bounded continental fragment

Abstract The complex structural setting of the western margin of North America is interpreted to result from oblique convergence of the North American and Pacific plates, accommodated by both right-lateral slip along the San Andreas fault and shortening east and west of it. Strike-slip movements along the San Andreas fault led to the detachment of a continental fragment (the Salinian Block) from the North American margin during early Miocene, and its translation northwestwards for over 300 km. Structural analysis in the Northern Salinian Block, west of the San Andreas fault, reveals a NE-SW-directed shortening accommodated by NW-SE-trending folds and thrusts with a dip-slip kinematic character. The stratigraphic record of progressively younger unconformities affected by both folds and thrusts, as well as the overprinting relationships among these contractional structures, enables us to clarify the tectonic evolution of the region since Paleocene time. The Paleocene to Recent history of the Salinian Block was dominated by strike-slip along the San Andreas fault, and by shortening perpendicular to it. The partitioning between strike-slip and dip-slip movements appears to be controlled by a pre-existing tectonic feature. The results from structural analysis along the Salinian Block are integrated into a deformation model for the western margin of North America, providing additional constraints on the timing of deformation and helping to clarify the role of strain-partitioning processes in the obliquely convergent California margin.
CLAVE ILUSTRADA PARA LA IDENTIFICACIÓN DE LOS TAXONES SUPRAESPECÍFICOS DE SIPHONAPTERA DE MÉXICO

Las 138 especies y 47 géneros mexicanos de pulgas (Siphonaptera) pertenecen a las siguientes familias y subfamilias: Rhopalopsyllidae (Rhopalopsyllinae), Pulicidae (Tunginae y Pulicinae), Ceratophyllidae (Dactylopsyllinae y Ceratophyllinae), Ischnopsyllidae (Ischnopsyllinae), Leptopsyllidae (Leptopsyllinae), Hystrichopsyllidae (Stenoponiinae, Rhadinopsyllinae, Neopsyllinae, Anomiopsyllinae, Hystrichopsyllinae y Doratopsyllinae) y Ctenophthalmidae (Ctenophthalminae). Se presenta una clave ilustrada para identificar las familias, subfamilias, tribus y géneros mexicanos de pulgas.
Lysine Acetyltransferase GCN5b Interacts with AP2 Factors and Is Required for Toxoplasma gondii Proliferation

Histone acetylation has been linked to developmental changes in gene expression and is a validated drug target of apicomplexan parasites, but little is known about the roles of individual histone modifying enzymes and how they are recruited to target genes. The protozoan parasite Toxoplasma gondii (phylum Apicomplexa) is unusual among invertebrates in possessing two GCN5-family lysine acetyltransferases (KATs). While GCN5a is required for gene expression in response to alkaline stress, this KAT is dispensable for parasite proliferation in normal culture conditions. In contrast, GCN5b cannot be disrupted, suggesting it is essential for Toxoplasma viability. To further explore the function of GCN5b, we generated clonal parasites expressing an inducible HA-tagged dominant-negative form of GCN5b containing a point mutation that ablates enzymatic activity (E703G). Stabilization of this dominant-negative GCN5b was mediated through ligand-binding to a destabilization domain (dd) fused to the protein. Induced accumulation of the ddHAGCN5b(E703G) protein led to a rapid arrest in parasite replication. Growth arrest was accompanied by a decrease in histone H3 acetylation at specific lysine residues as well as reduced expression of GCN5b target genes in GCN5b(E703G) parasites, which were identified using chromatin immunoprecipitation coupled with microarray hybridization (ChIP-chip). Proteomics studies revealed that GCN5b interacts with AP2-domain proteins, apicomplexan plant-like transcription factors, as well as a “core complex” that includes the co-activator ADA2-A, TFIID subunits, LEO1 polymerase-associated factor (Paf1) subunit, and RRM proteins. The dominant-negative phenotype of ddHAGCN5b(E703G) parasites, considered with the proteomics and ChIP-chip data, indicate that GCN5b plays a central role in transcriptional and chromatin remodeling complexes. We conclude that GCN5b has a non-redundant and indispensable role in regulating gene expression required during the Toxoplasma lytic cycle.
Synthesis, Anticancer Activity and UPLC Analysis of the Stability of Some New Benzimidazole-4,7-dione Derivatives

In this work, a sensitive analytical method to study the stability of two new series of synthesized heterocyclic compounds, the benzimidazole-4,7-diones 5 and N-oxide benzimidazole-4,7-dione derivatives 6 was established and validated. These derivatives were developed as potential anticancer substances to be activated under hypoxic conditions. At this point we were concerned with establishing their stability in some specific environments for further biological studies. For that, we developed and validated an RP-UPLC method. Next, selected compounds were tested in vitro for possible anticancer activity. Their effect on A549 tumour cell lines under normoxia and hypoxia conditions was determined by a WST-1 test. Four of the examined compounds (compounds 5a–c and 6c) showed very good antiproliferative effects and three of them (compounds 6a, 6b and 6d) were specific for hypoxia conditions. The hypoxia/normoxia cytotoxic coefficient of compound 6b is close to that of tirapazamine—a reference compound in our experiments—and this parameter locates it between mitomycin C and 2-nitroimidazole (misonidazole).
Petrology and P–T path of the Fuping mafic granulites: implications for tectonic evolution of the central zone of the North China craton

The Fuping Complex and the adjoining Wutai and Hengshan Complexes are located in the central zone of the North China craton. The dominant rock types in the Fuping Complex are high‐grade tonalitic–trondhjemitic–granodioritic (TTG) gneisses, with minor amounts of mafic granulites, syntectonic granitic rocks and supracrustal rocks. The petrological evidence from the mafic granulites indicates three stages of metamorphic evolution. The M1 stage is represented by garnet porphyroblasts and matrix plagioclase, quartz, orthopyroxene, clinopyroxene and hornblende. Orthopyroxene+plagioclase symplectites and clinopyroxene+plagioclase±orthopyroxene coronas formed in response to decompression during M2 following the peak metamorphism at M1. Hornblende+plagioclase symplectites formed as a result of further isobaric cooling and retrograde metamorphism during M3. The P–T estimates using TWQ thermobarometry are: 900–950 °C and 8.0–8.5 kbar for the peak assemblage (M1), based on the core compositions of garnet, matrix pyroxene and plagioclase; 700–800 °C and 6.0–7.0 kbar for the pyroxene+plagioclase symplectites or coronas (M2); and 550–650 °C and 5.3–6.3 kbar for the hornblende+plagioclase symplectites (M3), based on garnet rim and corresponding symplectic mineral compositions. These P–T estimates define a clockwise P–T path involving near‐isothermal decompression for the Fuping Complex, similar to the P–T path estimated for the metapelitic gneisses. The inferred P–T path suggests that the Fuping Complex underwent initial crustal thickening, subsequent exhumation, and finally cooling and retrogression. This tectonothermal path is similar to P–T paths inferred for the Wutai and Hengshan Complexes and other tectonic units in the central zone of the North China craton, but different from anti‐clockwise P–T paths estimated for the basement rocks in the eastern and western zones of the craton. Based on lithological, structural, metamorphic and geochronological data, the eastern and western zones of the craton are considered to represent two different Archean to Paleoproterozoic continental blocks that amalgamated along the central zone at the end of Paleoproterozoic. The P–T paths of the Fuping Complex and other tectonic units in the central zone record the collision between the eastern and western zones that led to the final assembly of the North China craton at c. 1800 Ma.
Assessing the accuracy of Landsat Thematic Mapper classification using double sampling

Double sampling was used to provide a cost efficient estimate of the accuracy of a Landsat Thematic Mapper (TM) classification map of a scene located in the Rocky Mountain National Park, Colorado. In the first phase, 200 sample points were randomly selected to assess the accuracy between Landsat TM data and aerial photography. The overall accuracy and Kappa statistic were 49.5 per cent and 32.5 per cent, respectively. In the second phase, 25 sample points identified in the first phase were selected using stratified random sampling and located in the field. This information was used to correct for misclassification errors associated with the first phase samples. The overall accuracy and Kappa statistic increased to 59.6 per cent and 45.6 per cent, respectively.
Development and Testing of a Groundwater Management Model for the Faultless Underground Nuclear Test, Central Nevada Test Area

This document describes the development and application of a user-friendly and efficient groundwater management model of the Central Nevada Test Area (CNTA) and surrounding areas that will allow the U.S. Department of Energy and state personnel to evaluate the impact of future proposed scenarios. The management model consists of a simple hydrologic model within an interactive groundwater management framework. This framework is based on an object user interface that was developed by the U.S. Geological Survey and has been used by the Desert Research Institute researchers and others to couple disparate environmental resource models, manage the necessary temporal and spatial data, and evaluate model results for management decision making. This framework was modified and applied to the CNTA and surrounding Hot Creek Valley. The utility of the management model was demonstrated through the application of hypothetical future scenarios including mineral mining, regional expansion of agriculture, geothermal energy production, and export of water to large urban areas outside the region. While the results from some of the scenarios indicated potential impacts to the region near CNTA and others did not, together they demonstrate the usefulness of the management tool for managers who need to evaluate the impact proposed changes in groundwatermore » use in or near CNTA may have on radionuclide migration. « less
Survival prediction of diffuse large-B-cell lymphoma based on both clinical and gene expression information

MOTIVATION It is important to predict the outcome of patients with diffuse large-B-cell lymphoma after chemotherapy, since the survival rate after treatment of this common lymphoma disease is <50%. Both clinically based outcome predictors and the gene expression-based molecular factors have been proposed independently in disease prognosis. However combining the high-dimensional genomic data and the clinically relevant information to predict disease outcome is challenging. RESULTS We describe an integrated clinicogenomic modeling approach that combines gene expression profiles and the clinically based International Prognostic Index (IPI) for personalized prediction in disease outcome. Dimension reduction methods are proposed to produce linear combinations of gene expressions, while taking into account clinical IPI information. The extracted summary measures capture all the regression information of the censored survival phenotype given both genomic and clinical data, and are employed as covariates in the subsequent survival model formulation. A case study of diffuse large-B-cell lymphoma data, as well as Monte Carlo simulations, both demonstrate that the proposed integrative modeling improves the prediction accuracy, delivering predictions more accurate than those achieved by using either clinical data or molecular predictors alone.
Massage treatment of hyperplasia of mammary glands

Abstract Background: With the accelerated pace of life, the problems of residence, diet, and environment have occurred frequently in recent years. External factors are easily to cause endocrine disorders and hormone sensitivity of breast tissue, which can lead to mammary hyperplasia. The incidence rate of hyperplasia of mammary glands is increasing year by year, and the age of onset is also getting lower and lower. If not treated in time, there is a crisis of breast cancer. Clinical studies have found that massage is widely used in clinical treatment of mammary hyperplasia recently, but the efficacy of massage in the treatment of mammary hyperplasia has not been systematically reviewed. The purpose of this study is to explore the efficacy, safety and effectiveness of massage in the treatment of hyperplasia of mammary glands. Methods: We will search PubMed, Cochrane Central Register of controlled trials (central), ScienceNet, EMBASE, CBM, CNKI, VIP and Wanfang databases. The retrieval date was October 20, 2020. RevMan 5.3 software was used to evaluate the quality and risk of included studies. The efficacy, recurrence rate, and symptom score of breast hyperplasia were analyzed, and the results were observed and measured. Results: This study will be from the clinical efficiency, improvement rate, pain symptoms disappear rate, tumor size improvement rate and other aspects of the existing evidence for a high quality synthesis, as well as massage adverse events. Conclusion: the conclusion of this review will provide the basis for judging whether massage is safe and effective in the treatment of hyperplasia of mammary glands. Ethics and dissemination: This systematic will evaluate the effectiveness and safety of massage in the treatment of hyperplasia of mammary glands. As all data used in this systematic review and meta-analysis have been published, ethical approval is not required for this review. Protocol registration number: INPLASY2020100066
On the role of iodine in ozone depletion

Ozone depletions in the lower stratosphere outside of polar regions are difficult to explain using only local chlorine and bromine chemistry. We speculate that iodine chemistry in combination with trends in anthropogenic chlorine and bromine may also be a factor in determining the widespread current depletion of lower stratospheric ozone. We also speculate on a related role for iodine in the sudden springtime surface ozone loss observed in the Arctic.
Acoustic waves in unsaturated soils

Seminal papers by Brutsaert (1964) and Brutsaert and Luthin (1964) provided the first rigorous theoretical framework for examining the poroelastic behavior of unsaturated soils, including an important application linking acoustic wave propagation to soil hydraulic properties. Theoretical developments during the 50 years that followed have led Lo et al., (2005) to a comprehensive model of these phenomena, but the relationship of its elasticity parameters to standard poroelasticity parameters measured in hydrogeology has not been established. In the present study, we develop this relationship for three key parameters, the Gassman modulus, Skempton coefficient, and Biot‐Willis coefficient by generalizing them to an unsaturated porous medium. We demonstrate the remarkable result that well‐known and widely applied relationships among these parameters for a porous medium saturated by a single fluid are also valid under very general conditions for unsaturated soils. We show further that measurement of the Biot‐Willis coefficient along with three of the six elasticity coefficients in the model of Lo et al. (2005) is sufficient to characterize poroelastic behavior. The elasticity coefficients in the model of Lo et al. (2005) are sensitive to the dependence of capillary pressure on water saturation and its viscous‐drag coefficients are functions of relative permeability, implying that hysteresis in the water retention curve and hydraulic conductivity function should affect acoustic wave behavior in unsaturated soils. To quantify these as‐yet unknown effects, we performed numerical simulations for Dune sand at two representative wave excitation frequencies. Our results show that the acoustic wave investigated by Brutsaert and Luthin (1964) propagates at essentially the same speed during imbibition and drainage, but is attenuated more during drainage than imbibition. Overall, effects on acoustic wave behavior caused by hysteresis become more significant as the excitation frequency increases.
Correlation between pressure gradient and phase saturation for oil‐water flow in smooth‐ and rough‐walled parallel‐plate models

The present study is aimed at an investigation of the pressure gradient/phase saturation correlation in the flow of an oil‐water mixture through a parallel‐plate (single fracture) model. It was found that the correlation depends on the interaction between the two flowing fluids, or flow pattern, which is in turn governed by the injection method, water flow rate, viscosity ratio, fracture aperture, and fracture surface roughness. Three distinct flow patterns were identified, namely, channel, dispersed, and mixed flows. Measurements of pressure gradient and phase saturation suggest that the widely used Romm's relative permeability relationship is applicable to channel flow in which oil and water phases are continuous in the fracture. For dispersed or mixed flow in which either the oil or water phase is discontinuous, a Lockhart‐Martinelli type correlation developed for gas‐liquid flow in pipes should be valid at low viscosity ratio but not at high viscosity ratio and water flow rate.
Amazonia and global change.

Amazonia and Global Change synthesizes results of the Large-Scale Biosphere-Atmosphere Experiment in Amazonia (LBA) for scientists and students of Earth system science and global environmental change. LBA, led by Brazil, asks how Amazonia currently functions in the global climate and biogeochemical systems and how the functioning of Amazonia will respond to the combined pressures of climate and land use change, such as: • Wet season and dry season aerosol concentrations, and their effects on diffuse radiation and photosynthesis • Increasing greenhouse gas concentration, deforestation, widespread biomass burning and changes in the Amazonian water cycle • Drought effects and simulated drought through rainfall exclusion experiments • The net flux of carbon between Amazonia and the atmosphere • Floodplains as an important regulator of the basin carbon balance including serving as a major source of methane to the troposphere • The impact of the likely increased profitability of cattle ranching The book will serve a broad community of scientists and policy makers interested in global change and environmental issues with high-quality scientific syntheses accessible to nonspecialists in a wide community of social scientists, ecologists, atmospheric chemists, climatologists, and hydrologists.
Complete Genome Sequence of Enterobacter cloacae subsp. cloacae Type Strain ATCC 13047

ABSTRACT Enterobacter cloacae is an important nosocomial pathogen. Here, we report the completion of the genome sequence of E. cloacae ATCC 13047, the type strain of E. cloacae subsp. cloacae. Multiple sets of virulence determinant and heavy-metal resistance genes have been found in the genome. To the best of our knowledge, this is the first complete genome sequence of the E. cloacae species.
53Mn‐53Cr ages of Kaidun carbonates

Abstract– We report the 53Mn‐53Cr systematics of three dolomite grains from two different CI1 clasts contained within the Kaidun meteorite breccia. Three internal isochrones result in initial 53Mn/55Mn ratios of (4.2 ± 0.4) × 10−6, (4.6 ± 1.3) × 10−6, and (5.2 ± 1.1) × 10−6. These initial values are consistent with those measured for dolomite in the Orgueil CI1 chondrite ( Hoppe et al. 2007 ; Petitat et al. 2009 ) but significantly lower than the initial ratio determined by Hutcheon et al. (1999) from a combination of different carbonate types within various lithologies of the Kaidun meteorite. We construct an accretion scenario for the Kaidun breccia by comparing the mineralogy and formation time scales of carbonates in the Kaidun CI1 lithologies to the analogous ones of the CI1 chondrite Orgueil. In Orgueil, dolomite precipitation precedes the formation of the first bruennerite grains by a few million years ( Hoppe et al. 2007 ; Petitat et al. 2009 ). As the CI1 clasts in Kaidun lack breunnerite grains, and considering that aqueous alteration occurred prior to reaccretion of the various clasts onto the Kaidun parent body (e.g., MacPherson et al. 2009 ), we hypothesize that after rapid accretion and early aqueous alteration occurring within the first approximately 4 Myr after solar system formation, impact disruption of several asteroids and their reassembly into the Kaidun parent asteroid was complete within an additional approximately 2 Myr. This confirms that aqueous alteration, impact, and reaccretion of material in the asteroid belt were early processes that began contemporaneously with chondrule formation.
Fusion of radar data to extract 3-dimensional objects LDRD final report

Interferometric Synthetic Aperture Radar (IFSAR) is a very promising technology for remote mapping of 3-Dimensional objects. In particular, 3-D maps of urban areas are extremely important to a wide variety of users, both civilian and military. However, 3-D maps produced by traditional optical stereo (stereogrammetry) techniques can be quite expensive to obtain, and accurate urban maps can only be obtained with a large amount of human-intensive interpretation work. IFSAR has evolved over the last decade as a mapping technology that promises to eliminate much of the human-intensive work in producing elevation maps. However, IFSAR systems have only been robustly demonstrated in non-urban areas, and have not traditionally been able to produce data with enough detail to be of general use in urban areas. Sandia Laboratories Twin Otter IFSAR was the first mapping radar system with the proper parameter set to provide sufficiently detailed information in a large number of urban areas. The goal of this LDRD was to fuse previously unused information derived from IFSAR data in urban areas that can be used to extract accurate digital elevation models (DEMs) over wide areas without intensive human interaction.