Miracle-dz/UniFAR-FTUs huggingface dataset:
Screening of lutein content in several fresh-water microalgae

Lutein is the most abundant xanthophylls in the photosynthetic organisms and its dietary intake is thought to protect against the development of age-related macular degeneration. Microalgae are predicted to be the major contributors of lutein in the future and to date, various species of microalgae has been analyzed for its lutein content. This study examined the lutein content of six chlorophycean microalgae species grown in the laboratory culture under the comparable environmental condition in which the population doubling time (t d ) as well as the maximum growth rate (μ max ) for each species was evaluated. Using batch culture operation grown under the heteroautotrophic condition, Scenedesmus dimorphus was observed to accumulate the highest lutein content (60.11 mg/g) with maximal biomass of 0.349 g/L culture. Based on the observations available, it appears that strains of unicellular microalgae of smaller size have appreciably higher intrinsic growth rates than algae of the larger size (Chlorella sp. B > Chlorella sp. TLL > Scenedesmus dimorphus > Scenedesmus obliquus TLL > Desmodesmus sp. TH > Ankistrodesmus sp. TH).
Named entity recognition and emotional viewpoint monitoring in online news using artificial intelligence

Network news is an important way for netizens to get social information. Massive news information hinders netizens to get key information. Named entity recognition technology under artificial background can realize the classification of place, date and other information in text information. This article combines named entity recognition and deep learning technology. Specifically, the proposed method introduces an automatic annotation approach for Chinese entity triggers and a Named Entity Recognition (NER) model that can achieve high accuracy with a small number of training data sets. The method jointly trains sentence and trigger vectors through a trigger-matching network, utilizing the trigger vectors as attention queries for subsequent sequence annotation models. Furthermore, the proposed method employs entity labels to effectively recognize neologisms in web news, enabling the customization of the set of sensitive words and the number of words within the set to be detected, as well as extending the web news word sentiment lexicon for sentiment observation. Experimental results demonstrate that the proposed model outperforms the traditional BiLSTM-CRF model, achieving superior performance with only a 20% proportional training data set compared to the 40% proportional training data set required by the conventional model. Moreover, the loss function curve shows that my model exhibits better accuracy and faster convergence speed than the compared model. Finally, my model achieves an average accuracy rate of 97.88% in sentiment viewpoint detection.
Hamilton cycles in bidirected complete graphs

Zaslavsky observed that the topics of directed cycles in directed graphs and alternating cycles in edge 2-colored graphs have a common generalization in the study of coherent cycles in bidirected graphs. There are classical theorems by Camion, Harary and Moser, Häggkvist and Manoussakis, and Saad which relate strong connectivity and Hamiltonicity in directed "complete" graphs and edge 2-colored "complete" graphs. We prove two analogues to these theorems for bidirected "complete" signed graphs.
Spike desensitisation as a mechanism for high-contrast selectivity in retinal ganglion cells

In the vertebrate retina, several dozens of parallel channels relay information about the visual world to the brain. These channels are represented by the different types of retinal ganglion cells (RGCs), whose responses are rendered selective for distinct sets of visual features by various mechanisms. These mechanisms can be roughly grouped into synaptic interactions and cell-intrinsic mechanisms, with the latter including dendritic morphology as well as ion channel complement and distribution. Here, we investigate how strongly ion channel complement can shape RGC output by comparing two mouse RGC types, the well-described ON alpha cell and a little-studied ON cell that is EGFP-labelled in the Igfbp5 mouse line and displays an unusual selectivity for stimuli with high contrast. Using patch-clamp recordings and computational modelling, we show that a higher activation threshold and a pronounced slow inactivation of the voltage-gated Na+ channels contribute to the distinct contrast tuning and transient responses in ON Igfbp5 RGCs, respectively. In contrast, such a mechanism could not be observed in ON alpha cells. This study provides an example for the powerful role that the last stage of retinal processing can play in shaping RGC responses.
Correlation of fatigue on walking ability in myasthenia gravis patients: a cross-sectional study

Introduction: Myasthenia gravis (MG) is a neuromuscular junction autoimmune disease characterised of intermittent muscle weakness that increases with activity and recovers with rest. Objective: Analysing the correlation of fatigue on walking ability in MG patients. Methods: This study used a cross-sectional design with consecutive sampling. Participants MG patients took in this trial. Data collection encompasses fatigue and walking ability, with fatigue being assessed using the fatigue severity scale (FSS) and walking ability being assessed using the 10-metre walking test. The 10-metre walking test assessment contains three components: comfortable walking speed (CWS), maximum walking speed (MWS), and natural cadence. The statistical analysis used in this study includes the Pearson correlation and Spearman rank tests with P<0.05. Results: The number of participants was 23 MG patients, and most of the participant was female (69.6%). The participant’s fatigue value was 5.46±1.13, including MGFA 1=5.32±1.15, MGFA 2A=5.5±1.11, and MGFA 2B=5.61±1.30. Meanwhile, the participant’s walking abilities included CWS of 1.10±0.11 m/s, MWS of 1.31±0.15 m/s, and natural cadence of 110.91±7.74 steps/min. No significant correlation of fatigue on walking ability including FSS vs. CWS (r=−0.141; P=0.520), FSS vs MWS (r=−0.169; P=0.442), and FSS vs. natural cadence (r=−0.050; P=0.822). Conclusion: There was no significant correlation between fatigue and walking ability in MG patients who had MGFA 1, MGFA 2A, and MGFA 2B.
Impact of SDN Controller’s Performance on Quality of Service

Software Defined Networking is a paradigm in network architecture; that is quickly becoming commonplace in modern telecommunication systems. It facilitates network customization for the requirements of different applications and simplifies the implementation of new services. Since its proposal, a significant evolution in its functionality has occurred. However, this development brought along problems of efficiency and performance, which are currently under research. A number of requirements has to be met, if Software Defined Networking is going to be the next step in the Next-Generation Networks progression. The central part of it – the SDN controller – has to put minimum strain on the system and provide performance which does not impede Quality of Service requirements. In this paper, the results of a research on SDN controller’s performance have been provided in the context of keeping up with flows’ QoS. For this, an emulated-physical research platform has been implemented. This research environment utilizes traffic generated accordingly to ITU-T recommendations to validate QoS parameters. The platform is given a thorough description. The results obtained from it take under consideration the implemented traffic sources, as well as the intensity of traffic handled by the controller and the traffic load of data plane links. Authors indicate that even without breaking the limitations set for delays in QoS, the impact of the controller workload is noticeable, which should be mitigated by applying appropriate resource control.
A Velocity Ambiguity Resolution Algorithm Based on Improved Hypothetical Phase Compensation for TDM-MIMO Radar Traffic Target Imaging

In principle, the imaging millimeter-wave radar based on time-division multiplexing multiple-input multiple-output (MIMO) technologies can provide richer target information for intelligent transportation systems due to the high-density target point cloud output. However, the chirp repetition interval extension reduces radar's inherent maximum detectable velocity, leading to the unavoidable problem of estimating the target velocity with a large ambiguity period in imaging radar moving target surveillance applications. To alleviate these problems, we propose an improved hypothetical phase compensation algorithm. Unlike the original method of determining the Doppler ambiguity period by comparing the peak amplitude of the angular power spectrum in each hypothetical case, the proposed algorithm selects the peak of the angular power signal-to-noise ratio (SNR) spectrum as the processing object and jointly decides the target speed by the average of the two highest wave peak intervals in the SNR variation curve. Simulations and practical experiments show that the improved algorithm has higher anti-interference performance. In particular, the proposed algorithm can remain continuously effective when multiple targets or angle information exist in the same distance Doppler cell, making it more suitable for MIMO imaging applications.
Research insights into the chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (CMTM): their roles in various tumors

The chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing (CMTM) family includes CMTM1–8 and CKLF, and they play key roles in the hematopoietic, immune, cardiovascular, and male reproductive systems, participating in the physiological functions, cancer, and other diseases associated with these systems. CMTM family members activate and chemoattract immune cells to affect the proliferation and invasion of tumor cells through a similar mechanism, the structural characteristics typical of chemokines and transmembrane 4 superfamily (TM4SF). In this review, we discuss each CMTM family member’s chromosomal location, involved signaling pathways, expression patterns, and potential roles, and mechanisms of action in pancreatic, breast, gastric and liver cancers. Furthermore, we discuss several clinically applied tumor therapies targeted at the CMTM family, indicating that CMTM family members could be novel immune checkpoints and potential targets effective in tumor treatment.
Nebulisation of Paclitaxel, Sotatercept and Iloprost for pulmonary hypertension for lung cancer. From In vitro to In vivo

Background: Pulmonary hypertension is common symptom among several diseases. The consequences are severe for several organs. Pulmonary hypertension is usually under-diagnosed and the main symptom observed is dyspnea with or without exercise. Currently we have several treatment modalities administered orally, via inhalation, intravenously and subcutaneously. In advanced disease then heart or lung transplantation is considered. The objective of the study was to investigate the optimum method of aerosol production for the drugs: iloprost, paclitaxel and the novel sotatercept. Materials and Methods: In our experiment we used the drugs iloprost, paclitaxel and the novel sotatercept, in an experimental concept of nebulization. We performed nebulization experiments with 3 jet nebulizers and 3 ultrasound nebulizers with different combinations of residual cup designs, and residual cup loadings in order to identify which combination produces droplets of less than 5μm in mass median aerodynamic diameter. Results: We concluded that paclitaxel cannot produce small droplets and is also still very greasy and possible dangerous for alveoli. However; iloprost vs sotatercept had smaller droplet size formation at both inhaled technologies (1.37<2.23 and 1.92<3.11, jet and ultrasound respectively). Moreover; residual cup designs C and G create the smallest droplet size in both iloprost and sotatercept. There was no difference for the droplet formation between the facemask and cone mouthpieces. Discussion: Iloprost and sotatercept can be administered as aerosol in any type of nebulisation system and they are both efficient with the residual cups loaded with small doses of the drug (2.08 and 2.12 accordingly).
Four novel Pleurocordyceps (Polycephalomycetaceae) species from China

Entomopathogenic fungi comprise an ecologically important group of specialized pathogens infecting other fungi, invertebrates, and plants. These fungi are species-rich with high diversity and broad distribution worldwide. The majority of entomopathogenic fungi belong to clavicipitoids, which consist of the hypocrealean families, Clavicipitaceae, Cordycipitaceae, Ophiocordycipitaceae, and Polycephalomycetaceae. The latter is a newly established entomopathogenic family that recently separated from the family Ophiocordycipitaceae to accommodate the genera, Perennicordyceps, Pleurocordyceps, and Polycephalomyces. In recent years, Polycephalomycetaceae has been enriched with parasitic and hyperparasitic fungi. With 16 species spread across China, Ecuador, Japan, and Thailand, Pleurocordyceps is the most speciose genus in the family. In this study, we expand the number of taxa in the genus by introducing four new Pleurocordyceps species from China, namely, P. clavisynnema, P. multisynnema, P. neoagarica, and P. sanduensis. We provide detailed descriptions and illustrations and infer genus-level phylogenies based on a combined 6-loci gene sequence dataset comprising the internal transcribed spacer gene region (ITS), small subunit ribosomal RNA gene region (SSU), large subunit rRNA gene region (LSU), translation elongation factor 1-alpha gene region (TEF-1α), RNA polymerase II largest subunit gene region (RPB1), and RNA polymerase II second largest subunit (RPB2). This study contributes to knowledge with regard to the diversity of Pleurocordyceps specifically and entomopathogenic Hypocreales more broadly.
Comparison between Platelet Lysate, Platelet Lysate Serum, and Fetal Bovine Serum as Supplements for Cell Culture, Expansion, and Cryopreservation

As cell culture supplements, human platelet lysate (PL) and human platelet lysate serum (PLS) are alternatives to fetal bovine serum (FBS) due to FBS-related issues such as ethical concerns, variability between batches, and the possible introduction of xenogenic contaminants. This study compared the composition and efficacy of PL, PLS, and FBS as supplements in the culture and cryopreservation of human dermal fibroblasts, Wharton’s jelly-derived mesenchymal stem cells (WJ-MCS), and adipose tissue (AdMSC). Biochemical components, some growth factors, and cytokines present in each of them were analyzed; in addition, the cells were cultured in media supplemented with 5% PL, 5% PLS, and 10% FBS and exposed to different freezing and thawing solutions with the supplements under study. Biochemical parameters were found to be similar in PL and PLS compared to FBS, with some differences in fibrinogen and calcium concentration. Growth factors and cytokines were higher in PL and PLS compared to FBS. Cell proliferation and morphology showed no significant differences between the three culture media. Regarding the cryopreservation and thawing of cells, better results were obtained with PLS and FBS. In conclusion, PL and PLS are an excellent choice to replace the standard supplement of animal origin (FBS) in the media used for the culture and cryopreservation of fibroblasts, WJ-MSC, and AdMSC.
INFLUENCE OF ENVIRONMENTAL ATTITUDES IN IRANIAN ECOTOURIST BEHAVIOUR

This study investigates the effects of environmental attitudes on pro-environmental behavior. A conceptual model examines the structural relations among environmental attitude, concern, value and knowledge towards pro environmental behavior of ecotourist. 439 Iranian ecotourists from Mashhad mountains were randomly surveyed. This study uses the structural equation model (SEM) to evaluate the factor that influence the pro-environmental behaviors. Results shows that pro-environmental concern, anthropocentric and ecocentric environmental attitude has a significant influence on pro-environmental behavior. Este estudio investiga los efectos de las actitudes medioambientales sobre el comportamiento proambiental. Un modelo conceptual examina las relaciones estructurales entre la actitud medioambiental, la preocupación, el valor y el conocimiento hacia el comportamiento proambiental del ecoturista. Se encuestó aleatoriamente a 452 ecoturistas iraníes de las montañas de Mashhad. Este estudio utiliza el modelo de ecuaciones estructurales (SEM) para evaluar los factores que influyen en los comportamientos proambientales. Los resultados muestran que la preocupación proambiental y la actitud medioambiental antropocéntrica y ecocéntrica influyen significativamente en el comportamiento proambiental.
Developing and Evaluating the Greenness of a Reliable, All-in-One Thin-Film Microextraction Protocol for Determining Fentanyl, Methadone, and Zolpidem in Plasma, Urine, and Oral Fluid

This paper proposes an all-in-one microextraction-based protocol capable of determining and quantifying fentanyl, methadone, and zolpidem in plasma, urine, and saliva at concentrations below those required by international regulatory organizations. A homemade thin-film microextraction device featuring an octyl–cyanopropyl stationary phase was coupled with LC-MS/MS. The proposed method was developed and validated according to FDA criteria, providing extraction efficiency values ranging from 26.7% to 76.2% with no significant matrix effects (2.6% to 15.5% signal suppression). The developed protocol provided low limits of quantification (mostly equal to 1 ng mL−1) and good reproducibility (intra- and inter-day RSDs of less than 9.6% and 12.0%, respectively) and accuracy (89% to 104% of the test concentration). An assessment of the protocol’s environmental impact indicated that attention must be devoted to eliminating the use of toxic reagents and developing its capability for in situ sampling and in-field analysis using portable instruments. The proposed TFME-based protocol provides clinical laboratories with a versatile, one-step tool that enables the simultaneous monitoring of fentanyl, methadone, and zolpidem using the most popular biological matrices.
Bayesian Quickest Change-Point Detection With an Energy Harvesting Sensor and Asymptotic Analysis

This paper studies the problem of the quickest change-point detection by a sensor powered by randomly available energy harvested from the environment under a Bayesian framework. In particular, the sensor observes a stochastic process by taking and processing samples at discrete times. We assume the distribution of the sampled data changes at an unknown random time, and the pre and post-change distributions are stationary and known. In the proposed framework, the sensor takes a new sample if there is enough evidence of a change. Otherwise, the sensor saves energy for the future and does not take new samples. The optimal policy is obtained by dynamic programming that minimizes the average detection delay for fixed upper bounds on the false alarm rate and an average number of samples taken before the change point. We model the test statistics as a perturbed random walk and study the asymptotic performance of the proposed method applying non-linear renewal theory under two different scenarios. First, <inline-formula><tex-math notation="LaTeX">$\bar{H}\geq E_{s}$</tex-math></inline-formula>, where <inline-formula><tex-math notation="LaTeX">$\bar{H}$</tex-math></inline-formula> is the average harvested energy in one sampling period and <inline-formula><tex-math notation="LaTeX">$E_{s}$</tex-math></inline-formula> is the energy needed to take and process a new sample, and second, <inline-formula><tex-math notation="LaTeX">$\bar{H}<E_{s}$</tex-math></inline-formula>. For the first scenario, the optional policy turns out to be greedy, <italic>i</italic>. <italic>e</italic>. , the sensor takes samples when sufficient energy is available. However, under the second scenario, the proposed method performs better than the greedy approach since it prepares for the future and uses available energy parsimoniously. We have provided several numerical results to support the derived theory.
The Effect of Implementing ESD in the PBL Model on Critical Thinking Ability in Environmental Pollution Material

Education for Sustainable Development (ESD) is an effort to realise the goals listed in the SDGs. ESD is an alternative approach to the field of education to prepare a generation that has an awareness of sustainable life. The purpose of this study is to analyze the effect of the application of the problem-based learning model on students' critical thinking skills on SDGs-oriented environmental pollution materials. The research method employed was a quasi-experimental with the control group pretest-posttest design. In determining the sample, the researchers employed purposive sampling techniques by considering the differences in academic achievement, differences between the two classes, and differences in the number of students. Therefore, class VII B with 27 students was chosen as the experimental class and class VII E with 25 students was chosen as the control class. The types of data used were quantitative. The pretest-posttest in the experimental class obtained an N-gain score of 0.58 in the medium criterion. The normality test in the experimental class obtained a score of Sig 0.48, which was lower than 0.05. Conversely, the control class obtained a score of Sig 0.20, which was lower than 0.05. The homogeneity result was Sig 0.12, which was lower than 0.05. The hypothesis testing using the independent-sample t-test, a Sig (2-tailed) value of 0.00 was obtained, and the results of the effect size test were 1.77 with the high criterion. Therefore, there was a significant effect of using the SDG-oriented PBL model towards critical thinking skills on the topic of environmental pollution.
Management of Juvenile Osteochondral Fractures Utilising Absorbable PLGA Implants

The incidence of articular injury, particularly osteochondral fractures (OCFs), has seen a cinnotable increase in recent years. Regardless of their location, fragments can be overlooked by plain radiographs, which might lead to osteoarthritis in the long run. Diagnostic imaging has a pivotal role in the assessment and classification of the fracture severity, as well as the presence of any associated dislocations. These fractures require surgical intervention for the restoration of joint function and the reduction of long-term complications. This paper aims to present the surgical correction and post-operative treatment of osteochondral fractures with absorbable implants in four children. The following affected areas are discussed: lateral condyle of the femur, patella and radial head. Utilising absorbable implants for the management of OCFs provides numerous advantages, including the elimination of the need for re-anaesthesia and reoperation, reduction of complications and facilitation of early rehabilitation. This approach also minimises the period of hospitalisation and proves effective in pediatric OCF treatment.