Miracle-dz/UniFAR-FTUs huggingface dataset:
NMR structure verifies the eponymous degenerate zinc finger domain of transcription factor ZNF750

ZNF750 is a nuclear transcription factor that activates skin differentiation and has tumor suppressor roles in several cancers. Unusually, ZNF750 has only a single zinc-finger (ZNF) domain, Z*, with an amino acid sequence that differs markedly from the CCHH family consensus. Because of its sequence differences Z* is classified as degenerate, presumed to have lost the ability to bind the zinc ion required for folding. AlphaFold predicts an irregular structure for Z* with low confidence. Low confidence predictions are often inferred to be intrinsically disordered regions of proteins, which would be the case if Z* did not bind Zn2+. We use NMR and CD spectroscopy to show that a 25-51 segment of ZNF750, corresponding to the Z* domain, folds into a well-defined antiparallel ββα tertiary structure with a pM dissociation constant for Zn2+, and a thermal stability >80 °C. Of three alternative Zn2+ ligand sets, Z* uses a CCHC rather than the expected CCHH motif. The switch in the last ligand maintains the folding topology and hydrophobic core of the classical ZNF motif. CCHC ZNFs are associated with protein-protein interactions but Z* binds DNA. Since the metal chelating site is on the other side of the molecule, it suggests functional preferences are a result of divergent evolution rather than physical constraints on the structure. The structure of Z* provides a context for understanding the domain’s DNA-binding properties and mutations associated with cancers. We expect other ZNFs currently classified as degenerate, are CCHC-type structures like Z*.
Post-acquisition super resolution for cryo-electron microscopy

Super resolution detector acquisition for cryo-EM has been used to improve the clarity of cryo-EM reconstructions. Recent reports have demonstrated achieving resolutions beyond the physical Nyquist limit using super resolution acquisition. Here, we demonstrate exceeding the physical Nyquist limitation by pre-processing the raw micrograph movies from “counting mode” data which has already reached physical Nyquist reconstruction resolution. To demonstrate functionality, micrograph movies of five datasets were pre-processed and demonstrate that it is possible to exceed the physical Nyquist limit via pixel doubling before motion correction. We call this “post-acquisition super resolution”, or PASR. While this was originally developed for processing of giant virus datasets, where acquiring at high magnification is not always possible or desirable, it is also shown to work for smaller objects such as adeno-associated virus (AAV) and apoferritin, both of which are still high symmetry, and jack bean urease, with lower symmetry. PASR could reduce the magnification required to achieve desired resolutions, which may increase collection efficiency. PASR can also be of use for in vivo tomography and facilities with high storage demands. However, this method should only be used for data which is able to achieve the Nyquist limit without PASR pre-processing. It will not improve attained resolutions of data which does not already reach the Nyquist limit.
13C metabolite tracing reveals glutamine and acetate as critical in vivo fuels for CD8+ T cells

Infusion of 13C-labeled metabolites provides a gold-standard for understanding the metabolic processes used by T cells during immune responses in vivo. Through infusion of 13C-labeled metabolites (glucose, glutamine, acetate) in Listeria monocytogenes (Lm)-infected mice, we demonstrate that CD8+ T effector (Teff) cells utilize metabolites for specific pathways during specific phases of activation. Highly proliferative early Teff cells in vivo shunt glucose primarily towards nucleotide synthesis and leverage glutamine anaplerosis in the tricarboxylic acid (TCA) cycle to support ATP and de novo pyrimidine synthesis. Additionally, early Teff cells rely on glutamic-oxaloacetic transaminase 1 (Got1)—which regulates de novo aspartate synthesis—for effector cell expansion in vivo. Importantly, Teff cells change fuel preference over the course of infection, switching from glutamine-to acetate-dependent TCA cycle metabolism late in infection. This study provides insights into the dynamics of Teff metabolism, illuminating distinct pathways of fuel consumption associated with Teff cell function in vivo. Teaser Interrogating dynamics of fuel utilization by CD8+ T cells in vivo reveals new metabolic checkpoints for immune function in vivo.
Young children’s negotiation of language policies and multilingual curriculum at an ethnic minority elementary school in rural China

Through interpreting the status of Hoche language and cultural practices from community voices, this ethnographic case study conducted in a minority language school centers on five Han and Hoche ethnic minority children’s positions toward multi-language learning. My research explores how these children negotiate the policies and multilingual curriculum that illuminates the multilayered tensions between local issues and global transformation. Findings indicate that policies situate Hoche as a romantic representation of the past, whereas the narratives I gathered from Hoche and Han children’s attitudes and ideologies about language show that younger generations are oriented toward future opportunities and influenced by globalization. This work contributes to scholarship on the education of minority children during a period of rapid industrialization by extending our understanding of ethnicity, diversity, and inclusivity issues via children’s perspectives. Shedding light onto the lives of Hoche and other ethnic minority children’s language practices, this paper is a call to urgently address power inequities in the ideologies and pedagogies enacted in service of Indigenous reclamation.
The Role of Financial Difficulties as a Mediator between Physical Symptoms and Depression in Advanced Cancer Patients

Financial difficulties experienced by cancer patients negatively impact the mental health of the patients. The objective of this study was to examine the mediating role of financial difficulties between physical symptoms and depression in patients with advanced cancer. A prospective, cross-sectional design was adopted in the study. The data were collected from 861 participants with advanced cancer in 15 different tertiary hospitals in Spain. The participants’ socio-demographic characteristics were collected using a standardized self-report form. Hierarchical linear regression models were used to explore the mediating role of financial difficulties. In the results, 24% of patients reported a high level of financial difficulties. Physical symptoms were positively associated with financial difficulties and depression (β = 0.46 and β = 0.43, respectively), and financial difficulties was positively associated with depression (β = 0.26). Additionally, financial difficulties played a role in explaining the relationship between physical symptoms and depression, showing a standardized regression coefficient of 0.43 which decreased to 0.39 after the financial difficulties were controlled. Healthcare professionals should consider the importance of providing financial resources and emotional support to help patients and their families cope with the financial burden associated with cancer treatment and its symptoms.
pleuropneumoniae serotype 5. for serodiagnosis of Actinobacillus enzyme-linked immunosorbent assay protocol Optimization and standardization of an

An indirect enzyme-linked immunosorbent assay protocol has been optimized with special emphasis given to assay standardization and quality control. Technical aspects such as choice of a microplate, antigen immobilization, buffer composition, optimal screening dilution of sera, and kinetics of the enzymatic reaction were studied and evaluated in order to design a standard protocol offering maximal analytical sensitivity and specificity, as well as to obtain minimal within- and between-plate variability. Among the 27 plates tested, the Nunc 475-094 and 269-620 immunoplates were found to be the best in terms of high positive-to-negative ratio and low variability. No significant differences in antigen immobilization were found by using buffers of various compositions or pHs; however, the presence of magnesium ions (Mg2+; 0.02 M) resulted in a twofold increase in nonspecific background. An optimal screening dilution of sera was established at 1:200. A 1-h incubation period for test serum was found to be optimal. Maximum enzymatic activity for peroxidase was obtained by adjusting both substrate (H202) and hydrogen donor [2,2'-azinobis(3-ethylbenz-thiazoline sulfonic acid)] concentrations to 4 and 1 mM, respectively. To control between-plate variability, a timing protocol was adopted. Within-plate variability was also controlled by using a sample placement configuration pattern. Sliding scales were determined by repeated testing of a cross section of samples to set acceptance limits for both within- and between-plate variability. These limits were used in a quality control program to monitor assay performance. The results obtained suggest that this standardized protocol might be useful in the serodiagnosis of Actinobacillus pleuropneumoniae serotype 5
S1366728922000657jra 293..306

A growing body of research suggests that bilingualism may afford benefits to certain aspects of cognitive functioning. Inconsistent findings may arise because of methodological differences within and across studies. One limitation is that studies often compare linguistically similar languages. The present study recorded brain activity (event-related potentials; ERPs) while English monolinguals, English–French bilinguals, and Arabic–English bilinguals completed an n-back task and a delayed matching-to-sample task. Group ERP differences were observed in the absence of behavioral differences. In the delayed matching-to-sample task, monolinguals exhibited smaller N2 amplitude compared to both bilingual groups, and smaller P3b amplitude compared to English–French bilinguals. In the n-back, English–French bilinguals displayed larger P3b amplitudes than monolinguals and Arabic–English bilinguals. P3b amplitude did not differ between Arabic–English bilinguals and monolinguals in either task. These results suggest that conflicting findings across studies may be due in part to the linguistic distance between the languages under study.
Breaking the Chains of Rationality: Understanding the Limitations to and Obtaining Order Policy Enforcement

Order manipulation attacks such as frontrunning and sand-wiching have become an increasing concern in blockchain applications such as DeFi. To protect from such attacks, several recent works have designed order policy enforcement (OPE) protocols to order transactions fairly in a data-independent fashion. However, while the manipulation attacks are motivated by monetary profits, the defenses assume honesty among a significantly large set of participants. In existing protocols, if all participants are rational , they may be incen-tivized to collude and circumvent the order policy without incurring any penalty. This work makes two key contributions. First, we explore whether the need for the honesty assumption is fundamental. Indeed, we show that it is impossible to design OPE protocols under some requirements when all parties are rational. Second, we explore the tradeoffs needed to circumvent the impossibility result. In the process, we propose a novel concept of rationally binding transactions that allows us to construct AnimaguSwap 1 , the first content-oblivious Automated Market Makers (AMM) that is secure under rationality.
Identification of Glucocorticoid Receptor Target Genes That Potentially Inhibit Collagen Synthesis in Human Dermal Fibroblasts

Over several decades, excess glucocorticoids (GCs) of endogenous or exogenous origin have been recognized to significantly inhibit collagen synthesis and accelerate skin aging. However, little is known regarding their molecular mechanisms. We hypothesized that the action of GCs on collagen production is at least partially through the glucocorticoid receptor (GR) and its target genes, and therefore aimed to identify GR target genes that potentially inhibit collagen synthesis in Hs68 human dermal fibroblasts. We first confirmed that dexamethasone, a synthetic GC, induced canonical GR signaling in dermal fibroblasts. We then collected 108 candidates for GR target genes reported in previous studies on GR target genes and verified that 17 genes were transcriptionally upregulated in dexamethasone-treated dermal fibroblasts. Subsequently, by individual knockdown of the 17 genes, we identified that six genes, AT-rich interaction domain 5B, FK506 binding protein 5, lysyl oxidase, methylenetetrahydrofolate dehydrogenase (NADP + dependent) 2, zinc finger protein 36, and zinc fingers and homeoboxes 3, are potentially involved in GC-mediated inhibition of collagen synthesis. The present study sheds light on the molecular mechanisms of GC-mediated skin aging and provides a basis for further research on the biological characteristics of individual GR target genes.
Consumer Preference of Traditional Korean Soy Sauce (Ganjang) and Its Relationship with Sensory Attributes and Physicochemical Properties

This study aimed to investigate the physicochemical characteristics, sensory attributes, and consumer acceptance of the Certification of Quality of Traditional Food (CQT) ganjang samples produced in different provinces of Korea. Wide variations in physicochemical properties were found among the samples, especially in lipids, total nitrogen, acidity, and reducing sugar. Traditional fermented foods are known to be closely tied to regional features, but the composition and characteristics of CQT ganjangs might be influenced much more by individual ganjang producers than by region. Preference mapping was performed to understand consumer behavior towards ganjang, and most consumers tended to have similar preferences, implying shared a common sensory ideal. The results of the partial least squares regression revealed drivers of liking for ganjang among sensory attributes, free amino acids, and organic acids. Overall, sensory attributes such as sweetness and umami were positively associated with acceptability, while the terms related to fermentation were negatively associated. In addition, amino acids, such as threonine, serine, proline, glutamate, aspartate, and lysine, and organic acids, such as lactate and malate, were positively associated with consumer acceptance. The important implications of the findings of this study for the food industry can be utilized to develop and optimize traditional foods.
Materialising Descent: Lineage Formation and Transformation in Early Neolithic Southern Britain

This paper builds on the recent aDNA results from Hazleton North chambered tomb to explore how people might have repeatedly negotiated kinship, descent, and affinity in Early Neolithic southern Britain. Hazleton North was constructed around 3700 cal bc, was in use for less than a century, and – unlike many other Cotswold-Severn tombs – was never modified to alter the arrangement of chambers. The aDNA analysis from 35 individuals whose remains were deposited at the site revealed that 27 were biologically related and represented five sequential generations. Here we explore changing practices across those generations. We argue that Hazleton North was constructed to demonstrate the vitality of a lineage at a specific moment in time while choices about who to entomb indicate an inclusive expansion of the lineage in the first two generations which is not evident during the remaining generations. We argue that by the third generation lineage members increasingly chose to dispose of the remains of their dead elsewhere. Hazleton North was built in a landscape rich in earlier tombs, many of which were modified to produce long cairns with multiple chambers: some of those formed opposed pairs similar to the chambered areas at Hazleton North. We argue this was part of a growing trend in ‘kinship work’ which accentuated lineal descent and sub-lineage distinctions in the centuries around 3700 cal bc. However, deposition at Hazleton North was short-lived. This can be set in the local context of not only the construction and use of further chambered tombs but also increasing investment in larger corporate projects like causewayed enclosures. These enclosures formed new arenas where negotiations of descent and community were played out with increased intensity and in different ways to activities at chambered tombs. Overall, we argue that kinship, affiliation, and belonging were repeatedly renegotiated among the monument building communities of Early Neolithic southern Britain.
An Empirical Guide for Visualization Consistency in Multiple Coordinated Views

Visual analytic systems usually provide multiple coordinated views (MCVs) to support data analysis and exploration. Coordination in visual graphics plays an important role in facilitating comprehensive analytical tasks, such as data comparison and cognitive inference. However, individual views in MCVs are probably designed for a specific purpose based on a particular type of data, and insufficient consideration of the intricate relationships among views may lead to inconsistency in visual representation and user interaction across different views. To better understand the inconsistency issues in MCVs and their impacts on user behaviors, this paper reports a study on the analysis and classification of visualization inconsistency based on the reviews of interactive visualization designs and visual analytic systems, and the interviews with stakeholders. We find that inconsistencies are prevalent in MCVs and frequently lead to misleading or even incorrect results. We classify the discovered inconsistencies based on a coordination model of MCVs, and develop an empirical guide for systematic and efficient visualization consistency checking in the design, implementation, and evaluation stage.
Attrition Rate Measuring In Human Resource Analytics Using Machine Learning

Nowadays, the majority of educational institutions try to improve the working conditions (job satisfaction, salary, workload, and work-life balance) of their employees so that they remain in their institutions for a long time. However, often in the same institutions, the number of resignations remains at about the same level. In such cases, it is important to identify the trends of such employees and prevent further resignations. This work aims to use Machine Learning (ML) algorithms for predicting the probable quitting of employees to help Human Resources (HR) henceforth retain valuable employees in advance, thereby benefiting both employees and the institution as a whole.
Exploitation of Hole Injection and Spreading for Dynamic Enhancement in p-GaN Gate HEMT under Room/High Temperatures

In this study, we investigate the role of hole injection and spreading in a p-GaN gate HEMT for addressing buffer-related dynamic $R_{\text{ON}}$ degradations. The proposed structure includes a buried AlGaN layer that acts as a hole barrier and provides a hole spreading channel (BHSC) under the 2DEG channel. During the on-state, holes are injected from the gate and spread along the BHSC. We observe that the hole injection is enhanced with increasing temperatures. The lateral spreading of holes is critical for suppressing buffer trapping outside of the gate region. As the suppression of buffer trapping is enhanced with an increase in $V_{\text{GS}}$, the dynamic $R_{\text{ON}}$ is dramatically reduced. We then adopt the widely used back-gating test to evaluate the dynamic $R_{\text{ON}}$ when buffer trapping is intentionally introduced. Finally, we demonstrate that the proposed device with BHSC exhibits nearly zero buffer-related dynamic $R_{\text{ON}}$ degradation from room temperature to 125°C.
Research on Business Integration Evaluation Management System of Modern Power Supply Enterprises

Centering on the goal of training the integrated talents and improving the quality and efficiency of enterprises, power supply enterprises have carried out active practice and exploration on business integration. In order to study the actual effect of business integration, form a set of scientific and applicable guidance methods for business integration of power grid companies, and promote the deepening development and application of integration, this study conducted an in-depth study on the status quo of integration. By analyzing external cases, doing internal status quo investigations, learning from some scientific theories, building quantitative models, studying development strategies and so on, to thoroughly interpret the development direction of integration, analyze the effectiveness and existing problems of integration, construct the evaluation and management system of DEI business integration, provide scientific guidance for the development of integration, and make innovative exploration for the evaluation of organizational effectiveness.
Experimental Demonstration of Point-Injection Trench IGBT Concept

The point injection trench IGBT is a promising concept based on the narrowing of the mesa between active gate trenches. In this paper, multiple design variations of the point injection trench IGBT with a voltage rating of 1200 V are fabricated and experimentally investigated. It is shown that sub micron mesa widths are successfully processed and a broad design space of IGBT devices with competitive performance is spanned.
Assessment of Stator Winding Insulation Condition Based on Absolute Partial Discharge Magnitude

Partial discharge (PD) measurement is widely used for all types of high voltage equipment for both factory quality assurance and insulation system condition assessment. For the latter, the PD may be measured off-line or on-line. For motor and generator stator windings in particular, on-line PD testing has been implemented on tens of thousands of machines using a wide variety of methods. How to perform such on-line tests is described in IEC and IEEE standards [1] , [2] . The usefulness of on-line testing has been described in many case study papers, and in particular, reference [3] summarizes the results of about 200 PD case studies presented by machine owners where stator failures were anticipated by the test, or where the findings of the on-line PD results were confirmed by visual inspections, therefore allowing proactive maintenance.
Location-Aware Beamforming Method for Time Varying Angle Of Departure in 5G mmWave Ultra-Dense Networks

The beamforming procedure is necessary in 5G New Radio (NR) Ultra-Dense Networks (UDN) to achieve high antenna array gain and overcome path losses in millimeter wave (mmWave) radio channels. In this regard, this article discusses antenna radiation pattern (ARP) formation or adaptive beamforming in order to orient beam between gNB (gNodeB) and user equipment (UE) properly and track it during UE movement in the scenario of time varying angle of departure (AOD) using uniform rectangular array (URA) at gNB side. Simulation results of AOD evaluation according to the considered method is close to true value and is comparable in accuracy with the beam cycling method which, requires 16 times longer time for training period, thus, making it appropriate for beam orientation in vehicle scenarios.