Miracle-dz/UniFAR-FTUs huggingface dataset:
Atomic Force Microscopy: A New Look at Pathogens

Microbial cells are highly complex and heterogeneous systems. In general, cell populations contain subgroups of cells which exhibit differences in growth rate as well as resistance to stress and drug treatment [1]. In addition, individual cells are spatially organized and heterogeneous, and this cellular heterogeneity is used to perform key functions [2]. This complexity emphasizes the need for single-cell analysis techniques in microbial research. Fluorescence imaging is a powerful tool to localize molecules in single cells [3], [4], but the resolution remains limited to the wavelength of the light source. On the other hand, high-resolution images of microbial structures can be obtained by electron microscopy techniques. In particular, cryo-electron tomography—or three-dimensional (3-D) electron microscopy—provides images of whole bacterial cells, at resolutions that are one to two orders of magnitude higher than those obtained with light microscopy [5]. In the past 20 years a new form of microscopy, atomic force microscopy (AFM), has revolutionized the way researchers probe the microbial cell surface. Instead of using an incident beam, AFM measures the minute forces acting between a sharp tip and the sample [6]–[8]. To generate a topographic image, the tip is attached to a cantilever that bends under force and is moved in three-dimensions using a piezoelectric scanner. While scanning the sample surface, cantilever's bending is measured by a laser beam focused on the free end of the cantilever and reflected into a photodiode. Unlike other microscopy techniques, 3-D images of cells and membranes are obtained at high resolution without staining, labelling or fixation, thus in physiological conditions. AFM is much more than a surface-imaging tool in that it also measures the localization and mechanical properties of the individual cell surface molecules. In this modality, known as single-molecule force spectroscopy, the cantilever deflection is recorded as a function of the vertical displacement of the scanner (as the sample is pushed towards the tip and it retracts) [6], [7]. This results in a cantilever deflection vs. scanner displacement curve, which is transformed into a force-distance curve using appropriate corrections. The characteristic adhesion force between tip and sample measured during retraction is used to probe the distribution and mechanics of single molecules, such as cell surface receptors. These novel AFM techniques complement traditional methods used to analyse microbial cell walls and provide new opportunities for understanding cell surface interactions. In this review, we provide a flavour of the various applications offered by AFM in microbiology, and we highlight some of the key breakthroughs the technique has enabled in pathogen research.
Accounting for Faculty Productivity in the Research University

Economic and political pressures have focused state legislative interest on faculty productivity. In Maryland, a negotiated approach resulted in a mission-sensitive policy that includes a reporting mechanism to measure instructional and noninstructional productivity. Legislative reporting requirements are being reduced as trust develops about the integrity of reporting and the management of faculty time. However, the two nonmedical research institutions continue to receive special scrutiny, with the legislature expecting to see increased teaching effort.
Moderators of and Mechanisms Underlying Stereotype Threat Effects on Older Adults' Memory Performance

Recent research has suggested that negative stereotypes about aging may have a detrimental influence on older adults' memory performance. This study sought to determine whether stereotype-based influences were moderated by age, education, and concerns about being stigmatized. Possible mechanisms underlying these influences on memory performance were also explored. The memory performance of adults aged 60 to 70 years and 71 to 82 years was examined under conditions designed to induce or eliminate stereotype threat. Threat was found to have a greater impact on performance in the young-old than in the old-old group, whereas the opposite was observed for the effects of stigma consciousness. In both cases, the effects were strongest for those with higher levels of education. Further analyses found little evidence in support of the mediating roles of affective responses or working memory. The only evidence of mediation was found with respect to recall predictions, suggesting a motivational basis of threat effects on performance. These findings highlight the specificity of stereotype threat effects in later adulthood as well as possible mechanisms underlying such effects.
A and E 1 B proteins inhibit inflammation induced by adenovirus

Replication-defective human adenovirus (Ad) group C transducing vectors, most of which have the E1A, E1B, and E3 genes deleted, are highly inflammatory despite the fact that the parental viruses typically cause subclinical or mild infections. To investigate this paradox, the roles that the E1A, E1B, and E3 genes play in inflammation were tested by using replication-incompetent viruses carrying a deletion of the preterminal protein gene. The viruses were injected into BALB c mouse ears, and edema was monitored as a sensitive surrogate marker of inflammation. A virus deleted for the E1A 289R (transcription activating) protein was noninflammatory, and inhibited edema induced by empty virus particles. The E1A 243R and E1B 55-kDa (p53 binding) proteins play the most important roles in inhibition of inflammation by the noninflammatory virus. The E1B 19-kDa antiapoptotic protein inhibited edema when both the E1A 243R and E1B 55-kDa proteins were expressed but strongly induced edema when only one was expressed. E3 proteins had their greatest effect on the inhibition of edema induced by the E1A 289R protein. The results support a model in which inflammation is countered through a mechanism that involves complex genetic interactions between Ad early region proteins and offer promise for the design and construction of noninflammatory Ad gene therapy vectors that are relatively easy to grow and purify.
Long-Distance Amplitude Correlations in the High Gamma Band Reveal Segregation and Integration within the Reading Network

Reading sentences involves a distributed network of brain regions acting in concert surrounding the left sylvian fissure. The mechanisms of neural communication underlying the extraction and integration of verbal information across subcomponents of this reading network are still largely unknown. We recorded intracranial EEG activity in 12 epileptic human patients performing natural sentence reading and analyzed long-range corticocortical interactions between local neural activations. During a simple task contrasting semantic, phonological, and purely visual processes, we found process-specific neural activity elicited at the single-trial level, characterized by energy increases in a broad gamma band (40–150 Hz). Correlation analysis between task-induced gamma-band activations revealed a selective fragmentation of the network into specialized subnetworks supporting sentence-level semantic analysis and phonological processing. We extend the implications of our results beyond reading, to propose that gamma-band amplitude correlations might constitute a fundamental mechanism for large-scale neural integration during high-level cognition.
The quality of life of children with pervasive developmental disorders.

AIM Quality of life is increasingly the focus of attention by health, psychological and social services. Pervasive developmental disorders (PDD) are a group of psychiatric conditions in which the patient's clinical case history is characterized by disturbances in social interaction, deterioration of verbal and non-verbal communication, and presence of bizarre, limited and stereotyped activity. These disturbances affect multiple developmental areas and show up in very early stages of development, resulting in a permanent disorder. Many studies have sought to recognize causes and interventions for persons with PDD, however, they often take insufficient account of the effects these disorder can have on the lifestyle of patients and their families. These clinical case histories are so pervasive that they cause a disorder which upsets the equilibrium of the person's entire life. The aim of this study was to assess the effect of living with PDD on the person's quality of life and to highlight the factors that impact on the person and his/her family. METHODS Both parents of 54 subjects (46 males and 8 females; age range 4-28 years) with diagnosed PDD (43 with autistic disorder, 2 with childhood disintegrative disorder, 3 with Asperger Syndrome, 6 with pervasive development disorder NAS) were enrolled in the study. The subjects affected with PDD were recruited at the AGSAS Onlus and IsMeC. Diagnosis was based on the criteria of the Diagnostic and Statistical Manual of Mental Disorders. Quality of life was assessed using the Italian version of the Impact of Childhood Illness Scale (Hoare and Russell, 1995). This scale consists of 30 questions that investigate the effect of illness on children, parents and families. For each question, the parent was asked to rate two variables: frequency and importance. Another questionnaire was administered to obtain medical history, diagnostic and therapeutic data of the persons with PDD. RESULTS Analysis of frequencies and percentages of questionnaire answers showed that the most important problems related to illness; specifically, according to the Frequency and Importance Parameters, the problems centered around self-care skills, difficulty in explaining the child's illness to others and looking after the child. The most important problems about the other children in the family concerned providing them with necessary attention and the restrictions their brother/sister's illness placed on their own activities. No significant correlations emerged between diagnosis type and answers on individual subscale items (Pearson's r). CONCLUSIONS Our data show that PDD has a considerable impact on both the child's development and the entire family. Parents' answers demonstrated that their child's illness had consequences for the child and how the family coped with it. For this reason, attention should be directed at psychological and social aspects, as well as attitudes, manners, reactions and effects such disturbances can have on the entire family.
Verification of dynamic process model change to support the adaptive workflow

As a technology that can improve the efficiency of the business process, workflow is drawing more and more attentions of researchers and software product vendors. But there are still many problems waiting to be solved for workflow. One of these problems is the poor adaptability of workflow. This paper intends to enhance the adaptability from the view of supporting dynamic process change. In the paper, a workflow model that is fit for dynamic change is defined and the verification rules are firstly set up for this model. Specifically, our method is based on the concept of executable path of workflow. Based on the executable path, the concepts and algorithms of valid process and complete subprocess are introduced to solve the problems brought by process model dynamic change.
Psoriasis and non‐alcoholic fatty liver disease

Several recent studies have found an increased prevalence of non‐alcoholic fatty liver disease within psoriasis patients. The exact pathophysiological mechanisms behind these observations are unclear, but are likely related to the high prevalence of obesity and metabolic syndrome within this patient population. Chronic inflammation, mediated by either proinflammatory adipokines or skin‐derived cytokines, may contribute to fatty liver disease development by increasing insulin resistance which in turn promotes hepatic lipid accumulation. These same adipokines in addition to hepatic cytokines may act on the skin to influence psoriasis disease severity.
Design and validation of a dynamic cell‐culture system for bone biology research and exogenous tissue‐engineering applications

Bone lacunocanalicular fluid flow ensures chemotransportation and provides a mechanical stimulus to cells. Traditional static cell‐culture methods are ill‐suited to study the intricacies of bone biology because they ignore the three‐dimensionality of meaningful cellular networks and the lacunocanalicular system; furthermore, reliance on diffusion alone for nutrient supply and waste product removal effectively limits scaffolds to 2–3 mm thickness. In this project, a flow‐perfusion system was custom‐designed to overcome these limitations: eight adaptable chambers housed cylindrical cell‐seeded scaffolds measuring 12 or 24 mm in diameter and 1–10 mm in thickness. The porous scaffolds were manufactured using a three‐dimensional (3D) periodic microprinting process and were composed of hydroxyapatite/tricalcium phosphate with variable thicknesses, strut sizes, pore sizes and structural configurations. A multi‐channel peristaltic pump drew medium from parallel reservoirs and perfused it through each scaffold at a programmable rate. Hermetically sealed valves permitted sampling or replacement of medium. A gas‐permeable membrane allowed for gas exchange. Tubing was selected to withstand continuous perfusion for > 2 months without leakage. Computational modelling was performed to assess the adequacy of oxygen supply and the range of fluid shear stress in the bioreactor–scaffold system, using 12 × 6 mm scaffolds, and these models suggested scaffold design modifications that improved oxygen delivery while enhancing physiological shear stress. This system may prove useful in studying complex 3D bone biology and in developing strategies for engineering thick 3D bone constructs. Copyright © 2013 John Wiley & Sons, Ltd.
External Memory Management and Simplification of Huge Meshes

Very large triangle meshes, i.e., meshes composed of millions of faces, are becoming common in many applications. Obviously, processing, rendering, transmission, and archiving of these meshes are not simple tasks. Mesh simplification and LOD management are a rather mature technology that, in many cases, can efficiently manage complex data. But, only a few available systems can manage meshes characterized by a huge size: RAM size is often a severe bottleneck. In this paper, we present a data structure called Octree-based External Memory Mesh (OEMM). It supports external memory management of complex meshes, loading dynamically in main memory only the selected sections and preserving data consistency during local updates. The functionalities implemented on this data structure (simplification, detail preservation, mesh editing, visualization, and inspection) can be applied to huge triangles meshes on low-cost PC platforms. The time overhead due to the external memory management is affordable. Results of the test of our system on complex meshes are presented.
In Vivo Comparison of the Bone Regeneration Capability of Human Bone Marrow Concentrates vs. Platelet-Rich Plasma

Background Bone marrow aspirate concentrate (BMAC) including high densities of stem cells and progenitor cells may possess a stronger bone regenerative capability compared with Platelet-rich plasma (PRP), which contains enriched growth factors. The objective of this study was to evaluate the effects of human BMAC and PRP in combination with β-tricalcium phosphate (β-TCP) on promoting initial bone augmentation in an immunodeficient mouse model. Methodology/Principal Findings BMAC and PRP were concentrated with an automated blood separator from the bone marrow and peripheral blood aspirates. β-TCP particles were employed as a scaffold to carry cells. After cell counting and FACS characterization, three groups of nude mice (BMAC+TCP, PRP+TCP, and a TCP control) were implanted with graft materials for onlay placement on the cranium. Samples were harvested after 4 weeks, and serial sections were prepared. We observed the new bone on light microscopy and performed histomorphometric analysis. After centrifugation, the concentrations of nucleated cells and platelets in BMAC were increased by factors of 2.8±0.8 and 5.3±2.4, respectively, whereas leucocytes and platelets in PRP were increased by factors of 4.1±1.8 and 4.4±1.9, respectively. The concentrations of CD34-, CD271-, CD90-, CD105-, and CD146-positive cells were markedly increased in both BMAC and PRP. The percentage of new bone in the BMAC group (7.6±3.9%) and the PRP group (7.2±3.8%) were significantly higher than that of TCP group (2.7±1.4%). Significantly more bone cells in the new bone occurred in sites transplanted with BMAC (552±257) and PRP (491±211) compared to TCP alone (187±94). But the difference between the treatment groups was not significant. Conclusions/Significance Both human BMACs and PRP may provide therapeutic benefits in bone tissue engineering applications. These fractions possess a similar ability to enhance early-phase bone regeneration.
A Family of High Power Factor Current Multilevel Rectifiers Employing a Sinusoidal PWM Technique

This work introduces the use of a sinusoidal pulse-width modulation (PWM) technique applied to two-stage single-phase current multilevel AC-to-DC converters. The use of a medium-frequency PWM strategy leads to a high power factor operation, with reduced harmonic content in the input current. Moreover, a safe distribution of the total current among the semiconductor switches is obtained, which is an inherent feature of the current multilevel converters. The DC-to-DC adopted converter stages employ a two-cell structure and draw a five-level sinusoidal PWM current from the AC utility. Along with theoretical and Pspice simulation results, the paper includes experimental details and waveforms from a 0.5 kW laboratory prototype which prove the practical feasibility regarding the present approach. It is expected that the proposed strategy can be applied to highly inductive DC load power systems
Local Knowledge, Environmental Politics, and the Founding of Ecology in the United States: Stephen Forbes and "The Lake as a Microcosm" (1887)

Stephen Forbes's "The Lake as a Microcosm" is one of the founding documents of the science of ecology in the United States. By tracing the connections between scientists and local fishermen underlying the research on floodplain lakes presented in "The Lake as a Microcosm," this essay shows how the birth of ecology was tied to local knowledge and the local politics of environmental transformation. Forbes and the other scientists of the Illinois Natural History Survey relied on fishermen for manual labor, expertise in catching fish, and knowledge of the natural history of the fishes. As Forbes and his colleagues worked in close contact with fishermen, they also adopted many of their political concerns over the privatization of the floodplain and became politically active in supporting their interests. The close connection between scientists and local knowledge forced the ecologists to reframe the boundaries of ecology as objective or political, pure or applied, local or scientific.
Emotion Improves and Impairs Early Vision

Recent studies indicate that emotion enhances early vision, but the generality of this finding remains unknown. Do the benefits of emotion extend to all basic aspects of vision, or are they limited in scope? Our results show that the brief presentation of a fearful face, compared with a neutral face, enhances sensitivity for the orientation of subsequently presented low-spatial-frequency stimuli, but diminishes orientation sensitivity for high-spatial-frequency stimuli. This is the first demonstration that emotion not only improves but also impairs low-level vision. The selective low-spatial-frequency benefits are consistent with the idea that emotion enhances magnocellular processing. Additionally, we suggest that the high-spatial-frequency deficits are due to inhibitory interactions between magnocellular and parvocellular pathways. Our results suggest an emotion-induced trade-off in visual processing, rather than a general improvement. This trade-off may benefit perceptual dimensions that are relevant for survival at the expense of those that are less relevant.
A Unified Statistical Model for Inter-Die and Intra-Die Process Variation

An efficient characterization technique with the spatial correlation matrix from electrical device parameters such as threshold voltage and saturation current accounting for inter- and intra-die variations is demonstrated. Then, a unified statistical model based on the correlation matrix is developed and implemented to the SPICE simulator to predict the distribution of circuit performance. In order to verify our model, test chips which consist of transistors and ring oscillators were fabricated using a 130nm CMOS technology. Simulated delay/skew variations of ring oscillators agree well with the measurement of test chips, maintaining a reasonable accuracy of 85 %. Especially, we show that as the distance of the two ring oscillators becomes larger, the timing skew between them becomes bigger. Moreover, the sensitivity analysis for the performance of simple analog and digital circuit, is performed in terms of inter-and intra-die variation.
Wider Horizons, Sharper Edges

I'he search for social universal.s lay at the core of earl classical social science. For some, contemporary social scientists globalization amounts to a new Euro-American universalism. However, what is most often over-looked in mainstream discourses on globalization is that whereas 'the universal' is invariant, globalization implies connectivity and hence variability. Global analysis currently needs to pay systematic attention to the dialectic between global connectivity and global variability.
Abnormal Positioning of Diencephalic Cell Types in Neocortical Tissue in the Dorsal Telencephalon of Mice Lacking Functional Gli3

The transcription factor Gli3 (glioma-associated oncogene homolog) is essential for normal development of the mammalian forebrain. One extreme requirement for Gli3 is at the dorsomedial telencephalon, which does not form in Gli3Xt/Xt mutant mice lacking functional Gli3. In this study, we analyzed expression of Gli3 in the wild-type telencephalon and observed a highdorsal-to-lowventral gradient of Gli3 expression and predominance of the cleaved form of the Gli3 protein dorsally. This graded expression correlates with the severedorsal-to-mildventral telencephalic phenotype observed in Gli3Xt/Xt mice. We characterized the abnormal joining of the telencephalon to the diencephalon and defined the medial limit of the dorsal telencephalon in Gli3Xt/Xt mice early in corticogenesis. Based on this analysis, we concluded that some of the abnormal expression of ventral telencephalic markers previously described as being in the dorsal telencephalon is, in fact, expression in adjacent diencephalic tissue, which expresses many of the same genes that mark the ventral telencephalon. We observed occasional cells with diencephalic character in the Foxg1 (forkhead box)-expressing Gli3Xt/Xt telencephalon at embryonic day 10.5, a day after the anatomical subdivision of the forebrain vesicle. Large clusters of such cells appear in the Gli3Xt/Xt neocortical region at later ages, when the neocortex becomes highly disorganized, forming rosettes comprising mainly neural progenitors. We propose that Gli3 is indispensable for formation of an intact telencephalic–diencephalic boundary and for preventing the abnormal positioning of diencephalic cells in the dorsal telencephalon.