Miracle-dz/UniFAR-FTUs huggingface dataset:
Increasing Realism and Variety of Virtual Patient Dialogues for Prenatal Counseling Education Through a Novel Application of ChatGPT: Exploratory Observational Study

Background Using virtual patients, facilitated by natural language processing, provides a valuable educational experience for learners. Generating a large, varied sample of realistic and appropriate responses for virtual patients is challenging. Artificial intelligence (AI) programs can be a viable source for these responses, but their utility for this purpose has not been explored. Objective In this study, we explored the effectiveness of generative AI (ChatGPT) in developing realistic virtual standardized patient dialogues to teach prenatal counseling skills. Methods ChatGPT was prompted to generate a list of common areas of concern and questions that families expecting preterm delivery at 24 weeks gestation might ask during prenatal counseling. ChatGPT was then prompted to generate 2 role-plays with dialogues between a parent expecting a potential preterm delivery at 24 weeks and their counseling physician using each of the example questions. The prompt was repeated for 2 unique role-plays: one parent was characterized as anxious and the other as having low trust in the medical system. Role-play scripts were exported verbatim and independently reviewed by 2 neonatologists with experience in prenatal counseling, using a scale of 1-5 on realism, appropriateness, and utility for virtual standardized patient responses. Results ChatGPT generated 7 areas of concern, with 35 example questions used to generate role-plays. The 35 role-play transcripts generated 176 unique parent responses (median 5, IQR 4-6, per role-play) with 268 unique sentences. Expert review identified 117 (65%) of the 176 responses as indicating an emotion, either directly or indirectly. Approximately half (98/176, 56%) of the responses had 2 or more sentences, and half (88/176, 50%) included at least 1 question. More than half (104/176, 58%) of the responses from role-played parent characters described a feeling, such as being scared, worried, or concerned. The role-plays of parents with low trust in the medical system generated many unique sentences (n=50). Most of the sentences in the responses were found to be reasonably realistic (214/268, 80%), appropriate for variable prenatal counseling conversation paths (233/268, 87%), and usable without more than a minimal modification in a virtual patient program (169/268, 63%). Conclusions Generative AI programs, such as ChatGPT, may provide a viable source of training materials to expand virtual patient programs, with careful attention to the concerns and questions of patients and families. Given the potential for unrealistic or inappropriate statements and questions, an expert should review AI chat outputs before deploying them in an educational program.
Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells

The anti-lung cancer properties of the plant Medicago orbicularis have not been explored yet. Therefore, we identified its phytochemical composition and investigated the antioxidant, anti-hemolytic, and anti-cancerous properties of extracts of this plant in A549 human lung adenocarcinoma cells. The results show that all parts of M. orbicularis (stems, leaves, and fruits) exhibit remarkable hemolytic activities and modest antioxidant capacity. In addition, all extracts showed a dose-dependent anti-cancerous cytotoxic activity against A549 cells, with fruit extracts being the most potent. This cytotoxic effect could be related, at least partly, to the induction of apoptosis, where M. orbicularis fruit extracts reduced the ratio of anti-apoptotic BCL-2/pro-apoptotic BAX, thereby promoting cellular death. Furthermore, the use of M. orbicularis, in combination with a conventional chemotherapeutic agent, cisplatin, was assessed. Indeed, the combination of cisplatin and M. orbicularis fruit extracts was more cytotoxic and induced more aggregation of A549 cells than either treatment alone. GC-MS analysis and total polyphenol and flavonoid content determination indicated that M. orbicularis is rich in compounds that have anti-cancerous effects. We propose M. orbicularis as a potential source of anti-cancerous agents to manage the progression of lung cancer and its resistance to therapy.
Investigating factors that lead to school dropout in Namibia

Although schools report a variety of reasons why learners leave school prematurely, these reasons do not reveal the underlying causes, especially multiple factors that influence learners’ attitudes, behaviours, and performance prior to dropping out. In order to understand the underlying causes behind learners’ decisions for dropping out, using a quantitative approach through document analysis this study first analysed the Education Management Information System (EMIS) reports for 2005 to 2009. The findings revealed that Kavango, Kunene and Omaheke regions were the regions with the highest dropout rates in the country. Using a qualitative approach, with a phenomenological design, the researchers interviewed school principals, school counsellors and teachers at randomly selected 20% of schools with the highest dropout rate for each region. This resulted into 58 schools in the Kavango region, 10 schools in the Kunene region and 5 schools in the Omaheke region. At the same schools, and using the snowball sampling procedure, the researchers selected and interviewed learners who have dropped out at some point during their schooling days but came back to school. The study found that, for all the learners interviewed, 50% of learners dropped out due to pregnancy, 20% dropped out due to economic factors, 15% due to system factors, 11% due to lack of parental involvement, and 4% due to cultural factors. On the basis of the findings a number of recommendations are made to mitigate the dropout phenomenon.
Employment Opportunity of Community College Students in Local Market Kirtipur

This paper seeks to explore the association between community college student and local market, to examine the relationship between community college and local market and to analyze the impact of hiring community college student. on local business sectors. The study uses primary sources and questionnaires to gather data on Community, community college student and local business houses in Kirtipur. Total population is 260, a sample size of 159 was used, with 16 questions categorized into five rating parameters: strongly disagree (SD), disagree (D), neither disagree nor agree (N), agree (A), and strongly agree (SA). The descriptive, inferential statistic and purposive sampling research design was followed. There is significant relationship between community college and local market. There is significant impact of hiring community college student on local business sectors. There is significant association between community college student and local market.
Characterizing relative humidity with respect to ice in midlatitude cirrus clouds as a function of atmospheric state

Midlatitude cirrus cloud macrophysical and microphysical properties have been shown in previous studies to vary seasonally and in various large‐scale dynamical regimes, but relative humidity with respect to ice (RHI) within cirrus clouds has not been studied extensively in this context. Using a combination of radiosonde and millimeter‐wavelength cloud radar data, we identify 1076 cirrus clouds spanning a 7 year period from 2004 to 2011. These data are separated into five classes using a previously published algorithm that is based largely on synoptic conditions. Using these data and classification scheme, we find that RHI in cirrus clouds varies seasonally. Variations in cirrus cloud RHI exist within the prescribed classifications; however, most of the variations are within the measurement uncertainty. Additionally, with the exception of nonsummer class cirrus, these variations are not statistically significant. We also find that cirrus cloud occurrence is not necessarily correlated with higher observed values of RHI. The structure of RHI in cirrus clouds varies more in thicker clouds, which follows previous studies showing that macrophysical and microphysical variability increases in thicker cirrus clouds.
Predictors of Multimorbidity (Defined as Diabetes + Hypertension) Amongst Males Aged 15–54 in India: An Urban/Rural Split Analysis

Objectives: This study aimed to determine which sociodemographic and lifestyle factors may act as predictors of multimorbidity (defined as diabetes + hypertension) amongst men aged 15–54 within urban and rural areas of India. Methods: Data from the latest 2019–2021 India NFHS-5 survey were utilized. Presumed cases of multimorbidity were defined as men who had DM + HTN. A total of 22,411 men in urban areas and 66,768 rural men were analyzed using mixed-effect multi-level binary logistic regression models. Results: Various predictors were found to have a statistically significant association to multimorbidity. Urban areas: Age, region of residence, wealth, religion, occupation, and BMI. Rural areas: Age, education, region of residence, wealth, occupation, caste, BMI, alcohol consumption, media exposure, and tobacco consumption. Conclusion: Departing from the broad operational definitions often studied within literature, this study provided insight into one of the most prevalent specific multimorbidities across India. The urban/rural split analyses revealed substantial differences in high-risk characteristics across both areas, which have commonly been overlooked. These findings may better inform policymakers and assist in effectively reducing multimorbidity-related burden through area-specific preventative programs.
Late Cretaceous Paraorthacodus (Palaeospinacidae, Neoselachii) from Sweden

Paraorthacodus conicus (Davis, 1890) of the family Palaeospinacidae, but previously believed to be a mako shark (family Lamnidae), and P. andersoni (Case, 1978), until now reported only from the Judith River Formation of Montana, are both described from the Late Cretaceous of the Kristianstad Basin, southern Sweden. The name Synechodus nerviensis Leriche, 1929, is a junior synonym of P. conicus and Synechodus striatus Case, 1978, is likewise a junior synonym of P. andersoni.
Simulating large emitters using CMAQ and a local scale finite element model: analysis in the surroundings of Barcelona

In this work, we compare two state of the art models to simulate large emitters in the local scale. The area of study is in the surroundings of Barcelona, where an important contributor to the SO2 levels is considered. The first modelling system uses the mesoscale meteorological model WRF-ARW, the Air Emission Model of Meteosim, AEMM, and the air quality model CMAQ. The second model is a one way nesting of the results from the first system in a subgrid Finite Element model; the results from the CMAQ simulation are used as initial and boundary conditions. The simulations have been carried out for one episode with high levels of SO2. The time period of the simulations is of 48h with a 24h spin-up.
On the emergence of rainfall extremes from ordinary events

The analysis and estimation of extreme event occurrences is a central problem in many fields of geoscience. Advancements in the study of extreme events have recently been limited, arguably in connection with asymptotic assumptions in the traditional extreme value theory (EVT) and with its focusing on a small fraction of the available observations representing the tail properties of the underlying event generation process. Here we develop a Metastatistical Extreme Value framework (MEV) which relaxes limiting assumptions at the basis of the traditional EVT and accounts for the full distribution of the underlying “ordinary” events. We apply this general approach to the relevant case of daily rainfall and find that the MEV approach reduces the uncertainty in the estimation of high‐quantile extremes by up to 50% with respect to the classical EVT. The improved predictive power of the MEV framework is connected with its recognizing that extremes emerge from repeated sampling of ordinary events, thereby being able to use all available observations.
Exploitation of Black Olive (Olea europaea L. cv. Piantone di Mogliano) Pomace for the Production of High-Value Bread

In this study, the morpho-textural features, total phenolic content (TPC), and antioxidant capacity (AOC) of bread fortified with olive (Olea europaea L.) pomace were evaluated. Fresh olive pomace was subjected to microbiological and chemical (TPC, AOC, and fiber) analyses; then, the same olive pomace was analyzed during 1 to 6 months of storage at 4 °C or −20 °C. All olive pomace samples were used in 10%, 15%, or 20% amounts to produce type 0 soft wheat (Triticum aestivum) and whole wheat bread samples. The volatile organic compounds (VOCs) in the bread samples were also analyzed to assess the effect of the addition of the olive pomace on the flavor profile of the baked products. The TPC and AOC evaluation of olive pomace showed no differences among the analyzed samples (fresh, refrigerated, or frozen). Regarding the bread containing olive pomace, the specific volume was not affected by the amount or the storage methods of the added pomace. Bread samples produced with soft wheat flour showed the lowest hardness values relative to those produced with whole wheat flour, irrespective of the amount or storage method of the olive pomace. Regarding color, the crust and crumb of the bread samples containing 20% olive pomace were significantly darker. The bread samples containing 20% olive pomace had the highest TPC. The bread samples with fresh olive pomace were characterized by terpenoids, ketones, and aldehydes, whereas the bread samples containing refrigerated olive pomace were characterized by alcohols (mainly ethanol), acids, esters, and acetate. Finally, the bread samples with frozen olive pomace showed a volatile profile similar to that of bread produced with fresh olive pomace. Olive pomace was shown to be a suitable ingredient for producing bread with high nutritional value.
Natural Products Biosynthesis by Streptomyces netropsis IMV Ac-5025 under Exogenous Sterol Action

Streptomycetes are known as producers of bioactive substances, particularly antibiotics. Streptomyces netropsis IMV Ac-5025 simultaneously produces different classes of antibiotics, including polyene compounds, phytohormones, and sterols, but the metabolic pathways involved in their biosynthesis are largely understudied. The aim of this work was to explore the biosynthesis of polyene antibiotics, sterols, and phytohormones when the producer is cultivated in a nutrient medium supplemented with exogenous β-sitosterol. Gas chromatography and high-performance liquid chromatography were applied to analyze the spectrum of bioactive compounds. The obtained results demonstrated not only an increase in the accumulation of biomass but also polyene antibiotics, intracellular sterols, auxins, and cytokinins, when cultivating S. netropsis IMV Ac-5025 in a liquid medium with the addition of β-sitosterol. The amount of biomass raised 1.5–2-fold, whilst the sum of polyene antibiotics increased 4.5-fold, sterols’ sum (ergosterol, cholesterol, stigmasterol, β-sitosterol, and 24-epibrassinolide) by 2.9-fold, auxins’ sum (indole-3-acetic acid, indole-3-acetic acid hydrazide, indole-3-carbinol, indole-3-butyric acid, indole-3-carboxaldehyde, and indole-3-carboxylic acid) by 6-fold, and cytokinins’ sum (zeatin, isopentyladenine, zeatin riboside, and isopentenyladenosine) by 11-fold. Thus, we put forward the hypothesis that β-sitosterol plays a regulatory role in the network of biosynthetic reactions of S. netropsis IMV Ac-5025.
Mindfulness meditation styles differently modulate source-level MEG microstate dynamics and complexity

Background The investigation of mindfulness meditation practice, classically divided into focused attention meditation (FAM), and open monitoring meditation (OMM) styles, has seen a long tradition of theoretical, affective, neurophysiological and clinical studies. In particular, the high temporal resolution of magnetoencephalography (MEG) or electroencephalography (EEG) has been exploited to fill the gap between the personal experience of meditation practice and its neural correlates. Mounting evidence, in fact, shows that human brain activity is highly dynamic, transiting between different brain states (microstates). In this study, we aimed at exploring MEG microstates at source-level during FAM, OMM and in the resting state, as well as the complexity and criticality of dynamic transitions between microstates. Methods Ten right-handed Theravada Buddhist monks with a meditative expertise of minimum 2,265 h participated in the experiment. MEG data were acquired during a randomized block design task (6 min FAM, 6 min OMM, with each meditative block preceded and followed by 3 min resting state). Source reconstruction was performed using eLORETA on individual cortical space, and then parcellated according to the Human Connect Project atlas. Microstate analysis was then applied to parcel level signals in order to derive microstate topographies and indices. In addition, from microstate sequences, the Hurst exponent and the Lempel-Ziv complexity (LZC) were computed. Results Our results show that the coverage and occurrence of specific microstates are modulated either by being in a meditative state or by performing a specific meditation style. Hurst exponent values in both meditation conditions are reduced with respect to the value observed during rest, LZC shows significant differences between OMM, FAM, and REST, with a progressive increase from REST to FAM to OMM. Discussion Importantly, we report changes in brain criticality indices during meditation and between meditation styles, in line with a state-like effect of meditation on cognitive performance. In line with previous reports, we suggest that the change in cognitive state experienced in meditation is paralleled by a shift with respect to critical points in brain dynamics.
Effect of Post-Weaning Concentrate Feeding Prior to Forage Finishing on Intramuscular Fat Deposition

Simple Summary Marbling or intramuscular fat deposition in beef is a major determinant of carcass quality and value. Providing high-grain diets during the finishing phase stimulates intramuscular fat deposition, and the carcass quality grade is higher when steers are corn-fed versus grass-fed. This study examined the efficacy of feeding high-concentrate diets for differing times during the post-weaning phase to enhance intramuscular fat deposition and improve carcass quality before forage finishing. Feeding concentrates for 120 day post-weaning enhanced intramuscular fat deposition by altering lipogenic/lipolytic gene expression during the post-weaning phase that was maintained during forage finishing. At slaughter, steers that were fed concentrates for 120 day had higher-quality grades at similar body weights and yield grades than the steers that were fed shorter time periods during the post-weaning phase. The polyunsaturated fatty acid content of the longissimus muscle at slaughter was altered with concentrate feeding post-weaning, but the overall ratio of n-6 to n-3 fatty acids was below the threshold regarded as beneficial for human health. Abstract The objectives of this study were to examine the effects of feeding high-concentrate diets post-weaning (PW) prior to forage finishing on (1) changes in ultrasound intramuscular fat deposition and lipogenic/lipolytic gene expression during the post-weaning phase and (2) carcass characteristics and fatty acid composition after forage finishing to 487 kg. Steers were randomly assigned to one of four treatments (PW0, PW40, PW80, and PW120) at weaning to examine the time of high-concentrate feeding prior to forage finishing. The ultrasound intramuscular fat content was greater (p < 0.05) for PW120 compared to those for PW0, PW40, or PW80 at the end of the post-weaning phase. Feeding high concentrates (PW120) up-regulated (p < 0.01) the mRNA expression of fatty acid transporters and lipogenic genes and down-regulated lipolytic genes in the LM compared to PW0. Carcasses from PW120 were graded 83% Choice (p = 0.025), whereas carcasses from other post-weaning treatments (PW0, 40, or 80) were graded 25, 36, and 54% Choice, respectively, at the final harvest. The total fatty acid content of the muscle at slaughter was greater (p = 0.0004) for PW120 than PW0, PW40, and PW80. Feeding high-concentrate diets to steers post-weaning for 120 day enhanced early intramuscular fat deposition without causing major changes to the fatty acid composition of the longissimus muscle after forage finishing.
Preferences for Tailored Support – Patients’ and Health Care Professionals’ Experiences Regarding Symptoms and Self-Management Strategies During the First Year After Curatively Intended Prostate Cancer Treatment

Purpose There is an increase in the number of men undergoing screening for prostate cancer, and advancements in treatments, which implies current knowledge about symptoms and self-management. This study aims to explore experiences of symptom distress, and self-management strategies during the first year after curatively intended treatment for prostate cancer, as identified by patients and health care professionals. Methods A qualitative design was used, including data triangulation from individual interviews with patients (n =17) and one focus group interview with healthcare professionals (n =5). Thematic analysis was used. Results The two main themes were identified: living with the consequences of treatment and navigating a new situation. Living with the consequences of treatment illustrated how losing control of bodily functions such as bladder, bowel, and sexual functions interfered with daily life. A stigma around the disease was described, and a life living in an unfamiliar body challenged ideas of masculinity. The first months after treatment ended was a distressing period related to the abruption in frequent contact with healthcare providers, and concerns about the future. The second theme, navigating a new situation, illustrates that self-management strategies varied, due to individual factors as did the need for tailored information and support provided from healthcare professionals and family, which was highly valued. Information and support were described as complex topics and healthcare professionals emphasized the need for appropriate education for staff to provide proper support to men after ended treatment. Conclusion Lingering symptoms and concerns were evident during the first year after treatment. Self-management strategies varied, and timely and tailored information and support during the first year were considered highly valued, important, and preferred by patients. Our results indicate that support should be offered immediately after curatively intended treatment.