Miracle-dz/UniFAR-FTUs huggingface dataset:
A symmetric prior knowledge based deep learning model for intracerebral hemorrhage lesion segmentation

Background: Accurate localization and classification of intracerebral hemorrhage (ICH) lesions are of great significance for the treatment and prognosis of patients with ICH. The purpose of this study is to develop a symmetric prior knowledge based deep learning model to segment ICH lesions in computed tomography (CT). Methods: A novel symmetric Transformer network (Sym-TransNet) is designed to segment ICH lesions in CT images. A cohort of 1,157 patients diagnosed with ICH is established to train (n = 857), validate (n = 100), and test (n = 200) the Sym-TransNet. A healthy cohort of 200 subjects is added, establishing a test set with balanced positive and negative cases (n = 400), to further evaluate the accuracy, sensitivity, and specificity of the diagnosis of ICH. The segmentation results are obtained after data pre-processing and Sym-TransNet. The DICE coefficient is used to evaluate the similarity between the segmentation results and the segmentation gold standard. Furthermore, some recent deep learning methods are reproduced to compare with Sym-TransNet, and statistical analysis is performed to prove the statistical significance of the proposed method. Ablation experiments are conducted to prove that each component in Sym-TransNet could effectively improve the DICE coefficient of ICH lesions. Results: For the segmentation of ICH lesions, the DICE coefficient of Sym-TransNet is 0.716 ± 0.031 in the test set which contains 200 CT images of ICH. The DICE coefficients of five subtypes of ICH, including intraparenchymal hemorrhage (IPH), intraventricular hemorrhage (IVH), extradural hemorrhage (EDH), subdural hemorrhage (SDH), and subarachnoid hemorrhage (SAH), are 0.784 ± 0.039, 0.680 ± 0.049, 0.359 ± 0.186, 0.534 ± 0.455, and 0.337 ± 0.044, respectively. Statistical results show that the proposed Sym-TransNet can significantly improve the DICE coefficient of ICH lesions in most cases. In addition, the accuracy, sensitivity, and specificity of Sym-TransNet in the diagnosis of ICH in 400 CT images are 91.25%, 98.50%, and 84.00%, respectively. Conclusion: Compared with recent mainstream deep learning methods, the proposed Sym-TransNet can segment and identify different types of lesions from CT images of ICH patients more effectively. Moreover, the Sym-TransNet can diagnose ICH more stably and efficiently, which has clinical application prospects.
Forage quality and physiological performance of mowed alfalfa (Medicago sativa L.) subjected to combined light quality and drought

Alfalfa (Medicago sativa) can dwell in water-deficient habitats, where it is difficult to predict dry mass (DM) production and forage quality due to understory transmittance. Mowing is a recommended practice for alfalfa populations under drought, but its effect on forested land receives less attention. In a controlled indoor experiment, we found that drought better reduces shoot DM weight and crude fiber content (CFi) in blue light (33.7% red, 48.5% green, and 17.8% blue lights) than red light (71.7% red, 13.7% green, and 14.6% blue lights). Mowing decreases carbon (C) isotope signature (δ13C), CFi, and total C content in shoots but increases their accumulations in DM, nonstructural carbohydrates, and crude fat content (CFa). The results also demonstrated that mown alfalfa has higher starch content when exposed to green light (26.2% red, 56.4% green, and 17.4% blue lights) compared to the other two spectra. Multiple factorial regression indicated that higher soluble sugar content accounted for the increase of CFa and DM weight for CFi. Overall, mowing in blue-light–enriched understory stands is recommended and produces high-forage–quality alfalfa, which can be used as a lowered crude fiber component.
The osteoblast secretome in Staphylococcus aureus osteomyelitis

Osteomyelitis (OM) is an infectious disease of the bone predominantly caused by the opportunistic bacterium Staphylococcus aureus (S. aureus). Typically established upon hematogenous spread of the pathogen to the musculoskeletal system or contamination of the bone after fracture or surgery, osteomyelitis has a complex pathogenesis with a critical involvement of both osteal and immune components. Colonization of the bone by S. aureus is traditionally proposed to induce functional inhibition and/or apoptosis of osteoblasts, alteration of the RANKL/OPG ratio in the bone microenvironment and activation of osteoclasts; all together, these events locally subvert tissue homeostasis causing pathological bone loss. However, this paradigm has been challenged in recent years, in fact osteoblasts are emerging as active players in the induction and orientation of the immune reaction that mounts in the bone during an infection. The interaction with immune cells has been mostly ascribed to osteoblast-derived soluble mediators that add on and synergize with those contributed by professional immune cells. In this respect, several preclinical and clinical observations indicate that osteomyelitis is accompanied by alterations in the local and (sometimes) systemic levels of both pro-inflammatory (e.g., IL-6, IL-1α, TNF-α, IL-1β) and anti-inflammatory (e.g., TGF-β1) cytokines. Here we revisit the role of osteoblasts in bacterial OM, with a focus on their secretome and its crosstalk with cellular and molecular components of the bone microenvironment and immune system.
Money lies and extramarital ties: Predicting separate and joint occurrences of financial deception and extramarital infidelity

Introduction Although spouses frequently financially deceive each other (MFD; i.e., marital financial deception), few studies have examined this relationship behavior. The purpose of our study is to examine predictors of separate and joint occurrences of MFD and extramarital affairs (EMI). We chose the predictors we tested using social exchange theory (SET). Methods We used a national sample of married individuals and multinomial logistic regression analyses to examine how different predictors were associated with membership in three different groups (MFD with no EMI, EMI with no MFD, and both MFD and EMI) relative to the group of participants who reported neither behaviors. Results Relationship satisfaction was associated with a lower likelihood of being in the MFD-only group, moral commitment was negatively associated with membership in both EMI groups, and personal dedication commitment was negatively associated with membership in both MFD groups. Flirting with someone other than one’s spouse was positively associated with being in all three groups relative to the reference group. The personal importance of religion was not associated with group membership. Discussion Moral commitment, personal dedication commitment, and flirting with someone other than one’s spouse predicted these two types of marital deception. It is likely that other issues that affect marital outcomes, comparisons, and monitoring alternatives to the relationship may predict MFD and/or EMI.
Pressure Derivative on Uncountable Alphabet Setting: a Ruelle Operator Approach

In this paper we use a recent version of the Ruelle-Perron-Frobenius Theorem to compute, in terms of the maximal eigendata of the Ruelle operator, the pressure derivative of some translation invariant spin systems. As an application of this result, we prove that the pressure of a Heisenberg-type model, on the lattice $\mathbb{N}\times \mathbb{Z}$, is Frechet differentiable, on a suitable Banach space. Additionally, exponential decay of the two-point function, for this model, is obtained for any positive temperature.
Autonomic measures identify stress, pain, and instability associated with retinopathy of prematurity ophthalmologic examinations

Background Retinopathy of prematurity (ROP) ophthalmologic examinations cause stress and pain. Infants’ stress and pain can be measured non-invasively using skin conductance (SC) and high frequency heart rate variability (HF-HRV), reflecting sympathetic-mediated sweating and parasympathetic activity, respectively. Objectives To test the utility of SC to detect sympathetic activation during ROP examination, and the contribution of HF-HRV to predict stability post-examination. Methods In this prospective, single center study, we measured SC continuously pre-, during, and post-examination, and HRV at 24 h pre-ROP examination. Clinical data included stability [apneas, bradycardias, and desaturations (A/B/Ds)], and interventions post-examination. Results SC increased 56% above baseline during ROP examination (p = 0.001) and remained elevated post-examination (p = 0.02). Post-hoc analysis showed higher illness acuity, represented by need for respiratory support, was associated with lower HF-HRV at 24 h pre-ROP examination (p = 0.001). Linear regression indicated lower HF-HRV at 24 h pre-examination contributed to the need for higher intervention (i.e., stimulation to breathe, oxygen support) particularly among infants with higher illness acuity [F(1, 15) = 5.05, p = 0.04; β = −1.33, p = 0.04]. Conclusion ROP examination induced a 2-fold increase in sympathetic activation which remained above baseline in recovery. Also, we propose that the low parasympathetic tone associated with autonomic imbalance contributes to instability and need for higher intervention to assure stabilization with A/B/D events. Our findings provide insight into the underestimation of adverse events associated with ROP examination and identification of infants who may be more vulnerable to potential sequelae following ROP examinations.
Evaluation of carbon balance and carbohydrate reserves from forced (Vitis vinifera L.) cv. Tempranillo vines

Elevated temperatures during berry ripening have been shown to affect grape quality. The crop forcing technique (summer pruning that ‘force’ the vine to start a new cycle) has been shown to improve berry quality by delaying the harvest date. However, yield is typically reduced on forced vines, which is attributed to vine low carbon availability soon after forcing and likely incomplete inflorescence formation. The present study aims to estimate the carbon balance of forced vines and evaluate vine responses to changes in carbon patterns due to forcing. Three treatments were studied on Tempranillo cultivar: non-forced vines (Control), vines forced shortly after fruit set (CFearly) and vines forced one month later at the beginning of bunch closure (CFlate). Whole canopy net carbon exchange was modelled and validated using two whole canopy gas exchange chambers. In addition, non-structural carbohydrate reserves at budburst, forcing date and harvest, were analysed. Yield, yield components and vegetative growth were also evaluated. Harvest date was delayed by one and two months in the CFearly and CFlate, respectively, which increased must acidity. However, yield was lower in the forced treatments compared to the Control (49% lower for CFearly and 82% for CFlate). In the second year, at the time when CFearly and CFlate dormant buds were unlocked (forced budburst), forced vines had significantly lower non-structural carbohydrates than Control vines at budburst. Although the time elapsed from budburst to reach maximum net carbon exchange was longer for the Control treatment (80 days) than for the forced treatments (about 40 days), average daily net carbon exchange until harvest was comparable between Control (60.9 g CO2/vine/day) and CFearly (55.9 g CO2/vine/day), but not for CFlate (38.7 g CO2/vine/day). In addition, the time elapsed from budburst to harvest was shorter in forced treatments (about 124 days) than for the Control (172 days). As a result, the cumulative net carbon exchange until harvest was reduced by 35% (CFearly) and 55% (CFlate) in the forced treatments. However, no differences in carbon reserves at harvest were observed between treatments partly helped by the higher source:sink ratio observed in forced than Control vines.
Integrated bioinformatic analysis identified a novel prognostic pan-programmed cell death signature for bladder cancer

Programmed cell death (PCD) refers to a molecularly regulated form of cell death that functions as an essential anticancer defense mechanism and serves as a target of anticancer therapies. Multiple types of PCD comprehensively regulate tumorigenesis and tumor progression and metastasis. However, a systemic exploration of the multiple types of PCD in cancers, especially bladder cancer, is lacking. In this study, we evaluated the expression pattern of genes associated with multiple types of PCD in bladder cancer using the “ssGSEA” method and conceptualized the multiple types of PCD as being collectively involved in “Pan-PCD”. Based on the differentially expressed genes related to Pan-PCD, we developed a Pan-PCD-related prognostic signature (PPRPS) to predict patient prognosis via univariate and multivariate Cox regression analysis. The PPRPS is an independent prognostic factor, and the AUC (Area Under Curve) for 3-year overall survival was 0.748. Combined with age and stage, PPRPS displayed excellent predictive ability. Based on the PPRPS, higher levels of immune cell infiltration, tumor microenvironment, and immune checkpoint molecules were observed in the high-PPRPS group. Furthermore, PPRPS enabled accurate risk prediction for metastatic urothelial carcinoma after anti-PD-L1 monoclonal antibody treatment. Patients in the high-PPRPS group had poor prognoses. Docetaxel, staurosporine, and luminespib were identified as potentially effective drugs for high-PPRPS bladder cancer patients. In summary, we developed the Pan-PCD signature to improve the accuracy of bladder cancer prognostic predictions and to provide a novel classification method to guide treatment selection.
Targeted alpha therapy with the 224Ra/212Pb-TCMC-TP-3 dual alpha solution in a multicellular tumor spheroid model of osteosarcoma

Osteosarcoma patients with overt metastases at primary diagnosis have a 5-year survival rate of less than 20%. TP-3 is a murine IgG2b monoclonal antibody with high affinity for an epitope residing on the p80 osteosarcoma cell surface membrane antigen. The tumor-associated antigen p80 is overexpressed in osteosarcomas, and has very low normal tissue expression. We propose a novel dual alpha targeting solution containing two radionuclides from the same decay chain, including the bone-seeking 224Ra, and cancer cell-surface seeking 212Pb-TCMC-TP-3 for the treatment of osteoblastic bone cancers, circulating cancer cells and micrometastases. In this in vitro study, the cytotoxic effects of 212Pb-TCMC-TP-3 (single alpha solution) and 224Ra/212Pb-TCMC-TP-3 (dual alpha solution) were investigated in a multicellular spheroid model mimicking micrometastatic disease in osteosarcoma. OHS spheroids with diameters of 253 ± 98 μm treated with 4.5, 2.7, and 3.3 kBq/ml of 212Pb-TCMC-TP-3 for 1, 4, and 24 h, respectively, were disintegrated within 3 weeks. The 212Pb-TCMC-TP-3 induced a 7-fold delay in spheroid doubling time compared to a 28-times higher dose with the non-specific 212Pb-TCMC-rituximab. The 224Ra/212Pb-TCMC-TP-3 completely disintegrated spheroids with diameters of 218–476 μm within 3 and 2 weeks after 4 and 24 h incubation with 5 kBq/ml, respectively. Treatment with 1 kBq/ml of 224Ra/212Pb-TCMC-TP-3 for 24 h caused an 11.4-fold reduction in spheroid viability compared with unconjugated 224Ra/212Pb. The single and dual alpha solutions with TP-3 showed cytotoxicity in spheroids of clinically relevant size, which warrant further testing of the dual alpha solution using in vivo osteosarcoma models.
Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum

Insect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum under abiotic stresses. Here, we identified five chitinase-like proteins (ApIDGF, ApCht3, ApCht7, ApCht10 and ApENGase), and clustered them into five groups (group II, III, V, Ⅹ, and ENGase). Developmental expression analysis revealed that the five A. pisum chitinase-related genes were expressed at whole developmental stages with different relative expression patterns. When aphids were exposed to various abiotic stresses including temperature, insecticide and the stress 20-hydroxyecdysone (20E), all five chitinase genes were differentially expressed in A. pisum. The results showed that insecticide such as imidacloprid down-regulated the expression of these five Cht-related genes. Analysis of temperature stress of A. pisum chitinase suggested that ApCht7 expression was high at 10°C, which demonstrates its important role in pea aphids under low temperature. Conversely, ApCht10 was more active under high temperature stress, as it was significantly up-regulated at 30°C. Besides, 20E enhanced ApCht3 and ApCht10 expression in A. pisum, but reduced ApCht7 expression. These findings provide basic information and insights for the study of the role of these genes under abiotic stress, which advances our knowledge in the management of pea aphids under multiple stresses.
What factors influence MOOC course completion? An investigation of course completion and workplace benefits from interpersonal attraction theory perspective

MOOCs have attracted millions of learners worldwide by providing the public with convenient access to learning and quality educational resources, but the high dropout rate in MOOCs is still an urgent problem. Drawing upon Situation-Organism-Behavior-Consequence framework and interpersonal attraction theory, this study investigates the relationship between instructor attractiveness and MOOC course completion and further analyzes the impact of MOOC course completion on their career development. The results reveal that knowledge attractiveness and communication attractiveness significantly influence learners’ thirst for knowledge. Communication attractiveness and physical attractiveness significantly influence learners’ parasocial relationships. The thirst for knowledge and parasocial relationships are important antecedents of course completion for in-service learners and in-service learners’ completion of MOOC courses positively affects their workplace benefits. The findings provide new perspectives for the research domain of online education.
Multiple wh-interrogatives in child heritage Romanian: On-line comprehension and production

This study compared the online comprehension and the production of multiple interrogatives in 18 Romanian-English bilingual children aged 6;0–9;2 (MAGE = 8;0) living in the UK who have Romanian as heritage language (L1) and English as majority language (L2) and 32 Romanian monolingual children aged 6;11 to 9;8 (MAGE = 8;3). We examined whether differences emerge between heritage and monolingual children in the online comprehension and in the production of multiple interrogatives in Romanian, which requires fronting of all wh-phrases, contrary to English. The main aim was to uncover to which extent similarities or differences in morphosyntactic properties between the L1 and the L2 systems affect the acquisition and processing of the heritage language/L1. Online comprehension was assessed in a self-paced listening task, while production was assessed using an elicitation task. The results reveal that Romanian heritage children show similar online comprehension patterns to monolingual children for multiple interrogatives in Romanian. A different pattern emerges for production as heritage children produce less complex multiple questions in Romanian and avoid movement of two wh-phrases in all elicited structures. Given that their predominant responses for multiple interrogatives only make use of the structural option present in English, namely one fronted wh-phrase and one in-situ, we take this to show that there is transfer from the majority language to the heritage language. Thus, language production in the children’s L1 seems to be affected by properties of the dominant L2, under cross-linguistic influence. Taken together, the results for both comprehension and production suggest that heritage children are able to establish the underlying representation of multiple wh-movement structures, similarly to monolinguals, but have difficulties activating the more complex structure in production.
In situ detection of alkaline phosphatase in a cisplatin-induced acute kidney injury model with a fluorescent/photoacoustic bimodal molecular probe

Kidneys play an important part in drug metabolism and excretion. High local concentration of drugs or drug allergies often cause acute kidney injury (AKI). Identification of effective biomarkers of initial stage AKI and constructing activable molecular probes with excellent detection properties for early evaluation of AKI are necessary, yet remain significant challenges. Alkaline phosphatase (ALP), a key hydrolyzing protease, exists in the epithelial cells of the kidney and is discharged into the urine following kidney injury. However, no studies have revealed its level in drug-induced AKI. Existing ALP fluorescent molecular probes are not suitable for testing and imaging of ALP in the AKI model. Drug-induced AKI is accompanied by oxidative stress, and many studies have indicated that a large increase in reactive oxygen species (ROS) occur in the AKI model. Thus, the probe used for imaging of AKI must be chemically stable in the presence of ROS. However, most existing near-infrared fluorescent (NIRF) ALP probes are not stable in the presence of ROS in the AKI model. Hence, we built a chemically stable molecular sensor (CS-ALP) to map ALP level in cisplatin-induced AKI. This novel probe is not destroyed by ROS generated in the AKI model, thus allowing high-fidelity imaging. In the presence of ALP, the CS-ALP probe generates a new absorbance peak at 685 nm and a fluorescent emission peak at 716 nm that could be used to “turn on” photoacoustic (PA) and NIRF imaging of ALP in AKI. Levels of CS-ALP build up rapidly in the kidney, and CS-ALP has been successfully applied in NIRF/PA bimodal in vivo imaging. Through the NIRF/PA bimodal imaging results, we demonstrate that upregulated expression of ALP occurs in the early stages of AKI and continues with injury progression.
CRISPR/Cas systems: Delivery and application in gene therapy

The CRISPR/Cas systems in prokaryotes such as bacteria and archaea are the adaptive immune system to prevent infection from viruses, phages, or other foreign substances. When viruses or phages first invade the bacteria, Cas proteins recognize and cut the DNA from viruses or phages into short fragments that will be integrated into the CRISPR array. Once bacteria are invaded again, the modified CRISPR and Cas proteins react quickly to cut DNA at the specified target location, protecting the host. Due to its high efficiency, versatility, and simplicity, the CRISPR/Cas system has become one of the most popular gene editing technologies. In this review, we briefly introduce the CRISPR/Cas systems, focus on several delivery methods including physical delivery, viral vector delivery, and non-viral vector delivery, and the applications of disease therapy. Finally, some problems in CRISPR/Cas9 technology have been proposed, such as the off-target effects, the efficiency of DNA repair mechanisms, and delivery of CRISPR/Cas system safely and efficiently to the target location.