Miracle-dz/UniFAR-FTUs huggingface dataset:
Tpr Misregulation in Hippocampal Neural Stem Cells in Mouse Models of Alzheimer’s Disease

Nuclear pore complexes (NPCs) are highly dynamic macromolecular protein structures that facilitate molecular exchange across the nuclear envelope. Aberrant NPC functioning has been implicated in neurodegeneration. The translocated promoter region (Tpr) is a critical scaffolding nucleoporin (Nup) of the nuclear basket, facing the interior of the NPC. However, the role of Tpr in adult neural stem/precursor cells (NSPCs) in Alzheimer’s disease (AD) is unknown. Using super-resolution (SR) and electron microscopy, we defined the different subcellular localizations of Tpr and phospho-Tpr (P-Tpr) in NSPCs in vitro and in vivo. Elevated Tpr expression and reduced P-Tpr nuclear localization accompany NSPC differentiation along the neurogenic lineage. In 5xFAD mice, an animal model of AD, increased Tpr expression in DCX+ hippocampal neuroblasts precedes increased neurogenesis at an early stage, before the onset of amyloid-β plaque formation. Whereas nuclear basket Tpr interacts with chromatin modifiers and NSPC-related transcription factors, P-Tpr interacts and co-localizes with cyclin-dependent kinase 1 (Cdk1) at the nuclear chromatin of NSPCs. In hippocampal NSPCs in a mouse model of AD, aberrant Tpr expression was correlated with altered NPC morphology and counts, and Tpr was aberrantly expressed in postmortem human brain samples from patients with AD. Thus, we propose that altered levels and subcellular localization of Tpr in CNS disease affect Tpr functionality, which in turn regulates the architecture and number of NSPC NPCs, possibly leading to aberrant neurogenesis.
Sulphur Isotope Abundances in Hydrocarbons and Source Rocks of Uinta Basin, Utah

The sulphur isotope abundances in the free asphalts, asphalts extracted from the supposed source rocks, and the inorganic sulphur compounds of the source rocks of the Uinta basin have been defermined. The results show that both the asphalts and inorganic sulphur compounds become more and more enriched in S34 with decreasing age and decreasing depth of the deposits, the youngest sediments being enriched 31 ^pmil as compared with 20 ^pmil for sea water sulphate. This one-to-one correspondence between S34/S32 ratio and age and depth of deposits is, in view of the changes in the nature of controlling environment which occurred, strong evidence in favor of a biological theory of S34 enrichment in inland lakes and seas. The free asphalt and asphalt extracted from the source rocks were found to have almost identical sulphur isotope ratios in the case of wurtzilite and albertite. However, in the case of the ozocerite, the asphalt extracted from the supposed source rock has quite a different S34/S32 ratio from that of free asphalt. These results confirm previously suggested relations except in the case of the ozocerite.
DSG-BTra: Differentially Semantic-Generalized Behavioral Trajectory for Privacy-Preserving Mobile Internet Services

While facilitating user daily lives, the booming development of mobile Internet services raises their privacy concerns because of the need to share travel trajectories. Due to the differences in access patterns and sensitive location attributes, behavioral semantics of user travel suffer from different degrees of leakage risks and have personalized privacy requirements. Semantic mobility-aware personalized privacy protection is still an open research issue in mobile scenarios. To this end, we propose a differentially semantic-generalized behavioral trajectory (DSG-BTra) for achieving privacy-preserving mobile Internet services. Specifically, we first explore the underlying behavioral patterns by formalizing user social mobility. Then, we evaluate the differential privacy sensitivity of user behavior to indicate the risks it faces. Finally, we generalize the behavioral semantics with a sensitivity-quantified strength and generate a DSG-BTra for the user to participate in mobile services. Extensive experiments with real-world data sets demonstrate DSG-BTra achieves flexible balance between privacy protection and application QoS, e.g., reducing the inference probability to 0.18–0.26 with a semantic similarity of 0.3–0.5.
Percepção de cuidadores sobre a qualidade de serviços de Fisioterapia Neuropediátrica em Centros de reabilitação no estado da Paraíba, Brasil

This study evaluates the perception of caregivers of the quality of neuropediatric physiotherapy services offered at specialized rehabilitation centers (SRC) in Brazil. This is a cross-sectional, quantitative, evaluative study whose target population was caregivers of children in rehabilitation. The AQSF-Neuroped instrument was used to evaluate the quality of three SRC based on the perception of 320 caregivers. The one-proportion hypothesis test was used based on each AQSF-Neuroped item and dimension. The Weight of Evidence and Latent Class Analysis models were used to evaluate the influence of variables on the perception of quality. A mean overall quality score of 133±14 points indicated a positive perception of quality; however, there were aspects related to physical structure (Interpersonal Dimension) and access (Organizational Dimension II) that needed to be improved (p-values
A toolbox for multi-temporal analysis of satellite imagery

In this paper we present a MATLAB toolbox that was developed for the analysis and classification of medium spatial resolution satellite imagery. Its main functionality is the time series analysis of satellite imagery for the land cover char- acterization. This multi-temporal assessment is of extremely high importance, since different land cover classes exhibit specific spectral reflectances as function of time and their exploration should significantly improve classification scores obtained from single date measurements. For the classification procedure of single date or multi-temporal imagery data we implemented the Support Vector Machine (SVM) algorithm. In addition to the multi- temporal analysis and classification tasks of satellite images the presented toolbox also includes procedures for sample collection of land cover classes and flag quality analysis of image pixels. This toolbox is the first version of an ongoing development effort that currently exploits MODIS images in TIF format. For further development, we plan to implement MERIS images.
LGBTQ+ youth experiences of the COVID-19 pandemic as observed through gender-sexuality alliances

Many of the factors that put LGBTQ+ youth at risk for poor mental health outcomes before the COVID-19 pandemic were exacerbated by the period of home confinement during the pandemic. The following exploratory study investigated the experiences of LGBTQ+ youth during the pandemic as perceived by Gender-Sexuality Alliance (GSA) advisors. In October-November 2021, recruitment began for a qualitative survey shared via a closed Facebook group for GSA advisors in a Midwestern state, in addition to an email sent by a nonprofit supporting GSAs in that state. Advisors were asked to complete the survey in reference to their experience facilitating these school clubs from March 2020-March 2021, a period during the pandemic that saw the highest number of restrictions around home confinement along with schools moving to a virtual format. Findings included themes reflecting impacts of the level of caregiver acceptance on access to support, youth engaging in support-seeking behaviors, and enhancements and challenges of virtual meetings. Implications for social work intervention are shared, focusing on supporting caregivers on a path toward acceptance of their child and advocating for policies that transform the culture and the conversation from whether LGBTQ+ youth can exist as themselves in some spaces, to fully embracing their identity as valued members of society.
Essential elements nurses have to address to promote a safe discharge in paediatrics: A systematic review and narrative synthesis

Abstract Aim The aim of the study was to synthesize the evidence on the essential elements, nurses must address when they perform therapeutic education to patients and their caregivers to promote a safe paediatric hospital‐to‐home discharge. Design A systematic review and narrative synthesis. Methods The search strategy identifies studies published between 2016 and 2023. The quality of the included studies was assessed using the Critical Appraisal Skills Programme checklists. The protocol of this review was not registered. A search of three electronic databases (PubMed, CINAHL and Web of Science) and a search in the reference lists of the included studies was conducted in February 2021 and June 2023. Results Fifteen studies met the inclusion criteria. The essential elements identified are grouped into the following topics: emergency management, physiological needs, medical device and medications management, long‐term management and short‐term management. Nurses have a critical role in ensuring patient safety and quality of care, and the nurses' competence makes the difference in the discharge's related outcomes. Our results can help the nursing profession implement comprehensive discharge projects. Our results support the improvement of nurse‐led paediatric discharge programmes. Nurse managers can identify the grey areas of therapeutic education provided in their units and work for their improvement. Following the implementation of therapeutic education on these topics, measuring the discharge's related outcomes could be interesting. This study addresses the problem of managing a safe and efficient nurse‐led discharge in a paediatric setting. It presents evidence on the essential elements to promote a safe paediatric discharge at home. These could impact nursing practice by using them to implement project and discharge pathways. We have adhered to relevant EQUATOR guidelines—PRISMA guidelines for reporting systematic review. No patients, service users, caregivers or public members were involved in this study due to its nature (systematic review).
Clinical screening of Nocardia in sputum smears based on neural networks

Objective Nocardia is clinically rare but highly pathogenic in clinical practice. Due to the lack of Nocardia screening methods, Nocardia is often missed in diagnosis, leading to worsening the condition. Therefore, this paper proposes a Nocardia screening method based on neural networks, aiming at quick Nocardia detection in sputum specimens with low costs and thereby reducing the missed diagnosis rate. Methods Firstly, sputum specimens were collected from patients who were infected with Nocardia, and a part of the specimens were mixed with new sputum specimens from patients without Nocardia infection to enhance the data diversity. Secondly, the specimens were converted into smears with Gram staining. Images were captured under a microscope and subsequently annotated by experts, creating two datasets. Thirdly, each dataset was divided into three subsets: the training set, the validation set and the test set. The training and validation sets were used for training networks, while the test set was used for evaluating the effeteness of the trained networks. Finally, a neural network model was trained on this dataset, with an image of Gram-stained sputum smear as input, this model determines the presence and locations of Nocardia instances within the image. Results After training, the detection network was evaluated on two datasets, resulting in classification accuracies of 97.3% and 98.3%, respectively. This network can identify Nocardia instances in about 24 milliseconds per image on a personal computer. The detection metrics of mAP50 on both datasets were 0.780 and 0.841, respectively. Conclusion The Nocardia screening method can accurately and efficiently determine whether Nocardia exists in the images of Gram-stained sputum smears. Additionally, it can precisely locate the Nocardia instances, assisting doctors in confirming the presence of Nocardia.
Identification of QTNs, QTN-by-environment interactions for plant height and ear height in maize multi-environment GWAS

Plant height (PH) and ear height (EH) are important traits associated with biomass, lodging resistance, and grain yield in maize. There were strong effects of genotype x environment interaction (GEI) on plant height and ear height of maize. In this study, 203 maize inbred lines were grown at five locations across China’s Spring and Summer corn belts, and plant height (PH) and ear height (EH) phenotype data were collected and grouped using GGE biplot. Five locations fell into two distinct groups (or mega environments) that coincide with two corn ecological zones called Summer Corn Belt and Spring Corn Belt. In total, 73,174 SNPs collected using GBS sequencing platform were used as genotype data and a recently released multi-environment GWAS software package IIIVmrMLM was employed to identify QTNs and QTN x environment (corn belt) interaction (QEIs); 12 and 11 statistically significant QEIs for PH and EH were detected respectively and their phenotypic effects were further partitioned into Add*E and Dom*E components. There were 28 and 25 corn-belt-specific QTNs for PH and EH identified, respectively. The result shows that there are a large number of genetic loci underlying the PH and EH GEIs and IIIVmrMLM is a powerful tool in discovering QTNs that have significant QTN-by-Environment interaction. PH and EH candidate genes were annotated based on transcriptomic analysis and haplotype analysis. EH related-QEI S10_135 (Zm00001d025947, saur76, small auxin up RNA76) and PH related-QEI S4_4 (Zm00001d049692, mads32, encoding MADS-transcription factor 32), and corn-belt specific QTNs including S10_4 (Zm00001d023333, sdg127, set domain gene127) and S7_1 (Zm00001d018614, GLR3.4, and glutamate receptor 3.4 or Zm00001d018616, DDRGK domain-containing protein) were reported, and the relationship among GEIs, QEIs and phenotypic plasticity and their biological and breeding implications were discussed.
Light from Shattered Worlds: Debris from Giant Impacts

Abstract Large impacts in the outer parts of a planetary system will produce debris discs that display a strong, distinctive, asymmetry, which will last for 105 year timescales. Debris resulting from a large impact may be able to explain the asymmetries in some known debris discs that have hitherto been difficult to understand.
A class of stochastic models for relating synoptic atmospheric patterns to regional hydrologic phenomena

A model for multistation precipitation, conditional on synoptic atmospheric patterns, is presented. The model, which we call the nonhomogeneous hidden Markov model (NHMM), postulates the existence of an unobserved weather state, which serves as a link between the large-scale atmospheric measures and the small-scale spatially discontinuous precipitation field. The weather state effectively acts as an automatic classifier of atmospheric patterns. The weather state process is assumed to be conditionally Markov, given the atmospheric data. The rainfall process is then assumed to be conditionally independent given the weather state. Various parameterizations for the weather state process and the rainfall process are discussed, and a likelihood-based estimation procedure is described. Model-based estimates of the storm duration distribution and first and second moments of the rainfall process are derived. As an example the model is fit to a four-station network of rain gauge stations in Washington state. The observed first and second moments are reproduced very closely. The fitted duration distributions are somewhat lighter tailed than the observed distribution at two of the four stations but provide a good fit at the other two. We conclude that the NHMM has promise as a method of relating synoptic atmospheric data to rainfall and other regional or local hydrologic processes.
The Research on Digital Tourism Services Platform Based on G/S Model Archietecture

The rapid development of spatial information technology makes it possible to combine spatial information with digital tourism.G/S model is a new type of spatial information network service model.Based on the framework system of the digital tourism services platform the practical method of constructing the digital tourism services platform is studied and presented,by means of G/S model.
Overview of the Integrated Global Radiosonde Archive

This paper provides a general description of the Integrated Global Radiosonde Archive (IGRA), a new radiosonde dataset from the National Climatic Data Center (NCDC). IGRA consists of radiosonde and pilot balloon observations at more than 1500 globally distributed stations with varying periods of record, many of which extend from the 1960s to present. Observations include pressure, temperature, geopotential height, dewpoint depression, wind direction, and wind speed at standard, surface, tropopause, and significant levels. IGRA contains quality-assured data from 11 different sources. Rigorous procedures are employed to ensure proper station identification, eliminate duplicate levels within soundings, and select one sounding for every station, date, and time. The quality assurance algorithms check for format problems, physically implausible values, internal inconsistencies among variables, runs of values across soundings and levels, climatological outliers, and temporal and vertical inconsistencies in temperature. The performance of the various checks was evaluated by careful inspection of selected soundings and time series. In its final form, IGRA is the largest and most comprehensive dataset of quality-assured radiosonde observations freely available. Its temporal and spatial coverage is most complete over the United States, western Europe, Russia, and Australia. The vertical resolution and extent of soundings improve significantly over time, with nearly three-quarters of all soundings reaching up to at least 100 hPa by 2003. IGRA data are updated on a daily basis and are available online from NCDC as both individual soundings and monthly means.
Growth, Mortality and Reproductive Biology of Narrow-bared Spanish mackerel Scomberomorus commerson (Lecepede, 1800) Along the Northern Tanzanian Coastal Waters

Scomberomorus commerson samples were collected monthly from April 2012 to August 2013 in the coastal waters of Dar es Salaam (DSM) and Pangani (PN), Tanzania. Growth parameters which included, mortality and spawning patterns were investigated. The estimated growth parameters of the von Bertalanffy function at DSM site was L∞ = 122.59 cm; K = 0.68. The corresponding parameters for the PN site were L∞ = 122.85 cm; K = 0.3. The total mortality estimates were Z = 2.7 yr -1 at DSM and 1.44 yr -1 at PN while natural mortality M was 0.74 and 0.43 yr-1, respectively. Fishing mortality (F) at DSM (1.77 yr-1) and PN (0.9 yr-1) was higher than Fopt and Flimit BRP; indicating that S. commerson along the northern coastal waters of Tanzania is being overexpled. Regional investigation of a number of biological population parameters and ichthyoplankton study is needed for a comprehensive stock assessment of S. commerson.
PFKFB3 is a critical regulator of neutrophil metabolism and function in rheumatoid arthritis

Neutrophils are key effector leukocytes of the innate immune system and play a pivotal role in defending the host against microbial infections. Recent studies have identified a crucial link between glycolysis and neutrophil cellular functions. Using human neutrophils, we have investigated the intricate relationship between glycolysis, extracellular glucose availability, and the enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), in the regulation of reactive oxygen species (ROS) and neutrophil extracellular trap (NET) production. We have identified that PFKFB3 activity is a key regulator of neutrophil ROS and NET production, cytotoxic molecules which are both implicated in the pathogenesis of immune-mediated inflammatory diseases such as rheumatoid arthritis (RA). Targeted inhibition of PFKFB3 expression blocked the production of ROS and NETs in a dose-dependent manner in both RA and HC neutrophils (p<0.01). RA neutrophils were more sensitive to lower concentrations of PFKFB3 inhibition. We also demonstrated that RA neutrophils retain ROS and NET production in culture conditions which mimic the low glucose environments encountered in the RA synovial joint. By dissecting the intricate interplay between PFKFB3, glycolysis, and neutrophil effector functions, this study advances the understanding of the molecular mechanisms governing innate immune responses and identifies PFKF3B as a potential therapeutic target for conditions characterized by dysregulated neutrophil activity.
Facilitators and barriers to implementation of a patient and staff reported measure for screening of palliative concerns of patients with heart failure: a qualitative analysis using the Consolidated Framework for Implementation Research

Background: Screening patients with patient-reported outcome measures allows identification of palliative care concerns. The Integrated Palliative Care Outcome Scale (IPOS) was developed in the United Kingdom for this purpose. Tools developed in another setting might not be readily usable locally. We previously evaluated the validity and reliability of the IPOS in our cardiology setting. However, it remains uncertain what factors would influence the subsequent implementation of IPOS for routine screening of patients with advanced heart failure in future practice. Objectives: This study aimed to identify the factors that could affect the IPOS implementation for patients with advanced heart failure. Design: This was a qualitative study conducted at the National Heart Centre Singapore. Methods: Patients with advanced heart failure who participated in our previous IPOS validation study were purposively recruited for semi-structured interviews. Healthcare workers caring for these patients and involved in the testing of the IPOS tool were also invited for interviews. The interviews were analyzed thematically and mapped to the Consolidated Framework for Implementation Research (CFIR). Results: Our analysis identified six potential facilitators and six potential barriers to implementation across five major domains of the CFIR (intervention characteristics, inner setting, outer setting, individual characteristics, and process). Facilitators include: (i) perception of utility, (ii) perception of minimal complexity, (iii) perception of relatability, (iv) conducive culture, (v) dedicated resources, and (vi) advocates for implementation. Barriers include: (i) need for adaptation, (ii) mindsets/role strains, (iii) resource constraints, (iv) cultural concerns, (v) individual needs, and (vi) change process. Conclusion: Institutions could focus on cultivating appropriate perceptions and conducive cultures, providing dedicated resources for implementation and introducing facilitators to advocate for implementation. Adaptation of IPOS to suit workflows and individual needs, consideration of change processes, and systemic changes to alleviate cultural, resource, and staff role strains would improve IPOS uptake during actual implementation in clinical services. Trial registration: Not applicable.