Miracle-dz/UniFAR-FTUs huggingface dataset:
Surface electromyographic evaluation of the neuromuscular activation of the inspiratory muscles during progressively increased inspiratory flow under inspiratory-resistive loading.

This study aimed to evaluate neuromuscular activation in the scalene and sternocleidomastoid muscles using surface electromyography (EMG) during progressively increased inspiratory flow, produced by increasing the respiratory rate under inspiratory-resistive loading using a mask ventilator. Moreover, we attempted to identify the EMG inflection point (EMGIP) on the graph, at which the root mean square (RMS) of the EMG signal values of the inspiratory muscles against the inspiratory flow velocity acceleration abruptly increases, similarly to the EMG anaerobic threshold (EMGAT) reported during incremental-resistive loading in other skeletal muscles. We measured neuromuscular activation of healthy male subjects and found that the inspiratory flow velocity increased by approximately 1.6-fold. We successfully observed an increase in RMS that corresponded to inspiratory flow acceleration with ρ ≥ 0.7 (Spearman's rank correlation) in 17 of 27 subjects who completed the experimental protocol. To identify EMGIP, we analyzed the fitting to either a straight or non-straight line related to the increasing inspiratory flow and RMS using piecewise linear spline functions. As a result, EMGIP was identified in the scalene and sternocleidomastoid muscles of 17 subjects. We believe that the identification of EMGIP in this study infers the existence of EMGAT in inspiratory muscles. Application of surface EMG, followed by identification of EMGIP, for evaluating the neuromuscular activation of respiratory muscles may be allowed to estimate the signs of the respiratory failure, including labored respiration, objectively and non-invasively accompanied using accessory muscles in clinical respiratory care.
Health Supplier Quality and the Distribution of Child Health

There is emerging evidence to suggest that initial differentials between the health of poor and more affluent children in the UK do not widen over early childhood. One reason may be that through the universal public funded health care system all children have access to equally effective primary care providers. This paper examines this explanation. The analysis has two components. It first examines whether children from poorer families have access to general practitioners of a similar quality to children from richer families. It then examines whether the quality of primary care to which a child has access has an impact on their health at birth and on their health during early childhood. The results suggest that children from poor families do not have access to markedly worse quality primary care, and further, that the quality of primary care does not appear to have a large effect on differentials in child health in early childhood.
Silencing BMI1 radiosensitizes human breast cancer cells by inducing DNA damage and autophagy

Overexpression of BMI1 in human cancer cells, a member of the polycomb group of repressive complexes, correlates with advanced stage of disease, aggressive clinico-pathological behavior, poor prognosis, and resistance to radiation and chemotherapy. Studies have shown that experimental reduction of BMI1 protein level in tumor cells results in inhibition of cell proliferation, induction of apoptosis and/or senescence, and increased susceptibility to cytotoxic agents and radiation therapy. Although a role for BMI1 in cancer progression and its importance as a molecular target for cancer therapy has been established, information on the impact of silencing BMI1 in triple-negative breast cancer (TNBC) and its consequence on radiotherapy have not been well studied. Therefore, in the present study we investigated the potential therapeutic benefit of radiation therapy in BMI1-silenced breast cancer cells and studied the mechanism(s) of radiosensitization. Human MDA-MB-231 and SUM159PT breast cancer cells that were either stably transfected with a lentiviral vector expressing BMI1 shRNA (shBMI1) or control shRNA (shControl) or transient transfection with a BMI1-specific siRNA were used. Silencing of BMI1 resulted in marked reduction in BMI1 both at the mRNA and protein level that was accompanied by a significant reduction in cell migration compared to control cells. Further, BMI1 knockdown produced a marked enhancement of DNA damage as evidenced by Comet Assay and γH2AX foci, resulting in a dose-dependent radiosensitization effect. Molecular studies revealed modulation of protein expression that is associated with the DNA damage response (DDR) and autophagy pathways. Our results demonstrate that BMI1 is an important therapeutic target in breast cancer and suppression of BMI1 produces radiation sensitivity. Further, combining BMI1-targeted therapeutics with radiation might benefit patients diagnosed with TNBC.
A PZT insulin pump integrated with a silicon micro needle array for transdermal drug delivery

Many of the compounds in drugs cannot be effectively delivered using current drug delivery techniques (e.g., pills and injections). Transdermal delivery is an attractive alternative, but it is limited by the extremely low permeability of the skin. Because the primary barrier to transport is located in the upper tissue, micro-electro-mechanical-system (MEMS) technology provides novel means in terms of both micro needles array and PZT pump, with the former one to increase permeability of human skin with efficiency, safety and painless delivery, and the latter one to decrease the size of the pump. Micro needles array has many advantages, including minimal trauma at penetration site due to the small size in needle, no condition limit, painless drug delivery for penetration depth with few nerves, and precise control of penetration depth for micro needle extension length. The micro needles array drug delivery is precise, painless, effective, clean and neatness, without any inconvenience. This promotes the development of biomedical sciences and technology and makes medical devices more humanized. So far most of the insulin pump has been using mechanical pump in clinical practices. We present the first development of this novel technology which can assemble the PZT pump and the micro needles together for diabetes mellitus. The micro needle array based on a flexible substrate can be mounted on non-planar surface or even on flexible objects such as a human fingers and arms. The PZT pump can pump the precision insulin much more accurately than the mechanical pump and the overall size is much smaller than those mechanical pumps. The hollow wall straight micro needles array is fabricated on a flexible substrate by deep-reactive ion etching (DRIE) and anisotropic wet etching techniques. The fabricated hollow micro needles are 200/spl mu/m in length and 30/spl mu/m in diameter. The micro needle array, which may be built with on-board fluid pumps, have potential applications in the chemical and biomedical fields for localized chemical analysis, programmable drug-delivery systems, and very small, precise fluids sampling. The micro needles array has been installed in an insulin pump for demonstration and a leak free packaging is introduced.
A Framework for Kernel-Based Multi-Category Classification

A geometric framework for understanding multi-category classification is introduced, through which many existing 'all-together' algorithms can be understood. The structure enables parsimonious optimisation, through a direct extension of the binary methodology. The focus is on Support Vector Classification, with parallels drawn to related methods. The ability of the framework to compare algorithms is illustrated by a brief discussion of Fisher consistency. Its utility in improving understanding of multi-category analysis is demonstrated through a derivation of improved generalisation bounds. It is also described how this architecture provides insights regarding how to further improve on the speed of existing multi-category classification algorithms. An initial example of how this might be achieved is developed in the formulation of a straightforward multi-category Sequential Minimal Optimisation algorithm. Proof-of-concept experimental results have shown that this, combined with the mapping of pairwise results, is comparable with benchmark optimisation speeds.
How far can luxury brands travel: avoiding the pitfalls of luxury brand extension

While there are almost as many opinions on fine wines as there are wines and wine critics, most would agree that Château Margaux, the famous Bordeaux first growth, is up there with the very best. It is a wine of which author William Styron (1992) wrote, in the novel Sophie’s Choice, ‘‘when you live a good life like a saint and die, that must be what they make you to drink in paradise’’ (p. 151). It would be a marketer’s dream to extend the Château Margaux brand. It could perhaps be broadened to less rare,more readily available early drinkingwines. Or, partnerships with wineries in other countries Business Horizons (2009) 52, 187—197
Properties and Applications of Crystalline Si1-x-yGexCy alloys

We have used both electrical and optical characterization methods to study the effect of carbon on the valence band offset of compressively strained Si1-x-yGexCy / Si (100) heterojunctions grown by Rapid Thermal Chemical Vapor Deposition (RTCVD) with substitutional C levels from 0% to 2.5%. Our work indicates that the change in the bandgap of Si1-x-yGexCy as carbon is added is entirely accommodated in the valence band. We also propose a simple model to predict the change of band structure with the incorporation of C. We have also investigated the transport properties of holes in Si1-x-yGexCy channels by fabricating a compressively strained Si1-x-yGexCy/Si (100) modulationdoped structures. We found that hole mobility decreased as more C was added. The decrease in hole mobility was determined to be caused by C-related defects, not by change in the effective mass of holes as C was added. Finally, we demonstrated an application of crystalline Si1-x-yGexCy by growing polycrystalline Si1-x-yGexCy and used it as part of a polycrystalline gate structure for PMOS devices. The results showed that the use of carbon in polycrystalline Si1-x-yGexCy suppressed boron penetration across the gate oxide. No effects of gate depletion with the use of poly-Si1-x-yGexCy were observed. Our work suggests that the addition of carbon reduced the chemical potential of boron in Si1-x-yGexCy, which deterred boron from diffusing across the underlying gate oxide.
Protective role of Osthole on myocardial cell apoptosis induced by doxorubicin in rats.

OBJECTIVE To explore the effect of Osthole on protecting myocardial cell apoptosis induced by doxorubicin during cardiac failure in rats. METHODS Myocardial cells isolated from the newborn SD rats were separated into three groups: cells treated with 1 μmol doxorubicin, cells treated with Osthole at three concentrations of 10, 20, and 40 μmol, cells treated neither with Osthole nor with doxorubicin were the control groups. Consequently, cell apoptosis of myocardial cells in each group was analyzed using TUNEL assay. Also, expressions of oxidase, NADPH, and ROS in myocardial cells were analyzed using different biological methods. Moreover, expressions of cell apoptosis associated proteins were analyzed using Western blotting. RESULTS Compared with the controls, the results showed that cells received Osthole and doxorubicin treatments performed high percentage of cell apoptosis, suggesting that Osthole could anesis myocardial cell apoptosis induced by doxorubicin (P<0.05). Osthole of 10 μmol depressed the expressions of cell apoptosis associated proteins including Caspase-3 and Cytc, and enhancing expression of Bcl-XL expression (P<0.05). Osthole of 20 μmol significantly decreased the generation of intracellar superoxidase, NADPH, and NADPH activity in myocardial cells treated with doxorubicin (P<0.05). Moreover, Osthole of 20 μmol could significantly increase phosphorylated elF2α level in cells. CONCLUSION Our study suggested that Osthole may play a protective role in suppressing myocardial apoptosis induced by doxorubicin through inhibiting NADPH and superoxidase production and downstream phosphorylated elF2α.
Genetic and Molecular Characterization of the VRN2 Loci in Tetraploid Wheat1[W][OA]

Winter wheat (Triticum spp.) varieties require long exposures to low temperatures to flower, a process called vernalization. The VRN2 locus includes two completely linked zinc finger-CCT domain genes (ZCCT1 and ZCCT2) that act as flowering repressors down-regulated during vernalization. Deletions or mutations in these two genes result in the elimination of the vernalization requirement in diploid wheat (Triticum monococcum). However, natural allelic variation in these genes has not been described so far in polyploid wheat (tetraploid Triticum turgidum and hexaploid Triticum aestivum). A tetraploid wheat population segregating for both VRN-A2 and VRN-B2 loci facilitated the characterization of different alleles. Comparisons between functional and nonfunctional alleles revealed that both ZCCT1 and ZCCT2 genes are able to confer vernalization requirement and that different ZCCT genes are functional in different genomes. ZCCT1 and ZCCT2 proteins from nonfunctional vrn2 alleles have mutations at arginine amino acids at position 16, 35, or 39 of the CCT domain. These positions are conserved between CCT and HEME ACTIVATOR PROTEIN2 (HAP2) proteins, supporting a model in which the action of CCT domains is mediated by their interactions with HAP2/HAP3/HAP5 complexes. This study also revealed natural variation in gene copy number, including a duplication of the functional ZCCT-B2 gene and deletions or duplications of the complete VRN-B2 locus. Allelic variation at the VRN-B2 locus was associated with a partially dominant effect, which suggests that variation in the number of functional ZCCT genes can be used to expand allelic diversity for heading time in polyploid wheat and, hopefully, improve its adaptation to different environments.
Conservation of Farmland Birds Faces Different Challenges in Western and Central-Eastern Europe

Abstract. Birds are commonly used as an example of the strongly declining farmland biodiversity in Europe. The populations of many species have been shown to suffer from intensification of management, reduction of landscape heterogeneity, and habitat loss and fragmentation. These conditions particularly dominate farmland in the economically well developed countries of Western Europe. Currently, the farmland environment in Central-Eastern Europe is generally more extensive than in Western Europe and a larger proportion of people still live in rural areas; thus generating different conditions for birds living in agricultural areas. Furthermore, the quasi-subsistence farming in much of Central-Eastern Europe has resulted in agricultural landscapes that are generally more complex than those in Western Europe. To protect declining bird populations living in farmland, detailed knowledge on both species and communities is necessary. However, due to scientific tradition and availability of funding, the majority of studies have been carried out in Western Europe. In consequence this provokes a question: are findings obtained in western conditions useful to identify the fate of farmland bird biodiversity in Central-Eastern Europe? Therefore, the major goal of this paper is to highlight some local and regional differences in biodiversity patterns within EU farmland by comparing intensive agricultural landscapes with more extensive ones. More specifically, we aim to outline differences in agricultural landscapes and land use history in the two regions, use farmland birds to provide examples of the differences in species dynamics and species-habitat interactions between the two regions, and discuss possible social and ecological drivers of the differences in the context of biodiversity conservation. Factors governing spatio-temporal dynamics of farmland bird populations may differ in intensive and extensive landscapes as illustrated here using the Grey Partridge Perdix perdix and the Red-backed Shrike Lanius collurio as examples. The unevenness of farmland bird studies distribution across Europe was also presented. We call for more emphasis on pluralism in furthering both pan-European research on farmland bird ecology and conservation strategies. We also highlight some features specific to Central-Eastern Europe that merit consideration for the more efficient conservation of farmland birds and farmland biodiversity across Europe.
Essential n-3 fatty acids in pregnant women and early visual acuity maturation in term infants.

BACKGROUND Docosahexaenoic acid (DHA) is important to neural development. Whether DHA intakes are low enough in some pregnant women to impair infant development is uncertain. OBJECTIVE We sought to determine whether DHA deficiency occurs in pregnant women and contributes to poor infant development. DESIGN Biochemical cutoffs, dietary intakes, or developmental scores indicative of DHA deficiency are not defined. Infant development has a distribution in which an individual's potential development is unknown. This was a randomized intervention to establish a distribution of developmental scores for infants of women with DHA intakes considered to be above requirements against which to compare the development of infants of mothers consuming their usual diet. DHA (400 mg/d; n = 67) or a placebo (n = 68) was consumed by the women from 16 wk gestation until delivery. We determined maternal red blood cell ethanolamine phosphoglyceride fatty acids, dietary intakes at 16 and 36 wk gestation, and infant visual acuity at 60 d of age. RESULTS We described an approach to identify DHA deficiency when biochemical and functional markers of deficiency are unknown. In multivariate analyses, infant visual acuity was related to sex (beta = 0.660, SE = 0.93, and odds ratio = 1.93) and maternal DHA intervention (beta = 1.215, SE = 1.64, and odds ratio = 3.37). More infant girls in the placebo than in the DHA intervention group had a visual acuity below average (P = 0.048). Maternal red blood cell ethanolamine phosphoglyceride docosatetraenoic acid was inversely related to visual acuity in boys (rho = -0.37, P < 0.05) and girls (rho = -0.48, P < 0.01). CONCLUSIONS These studies suggest that some pregnant women in our study population were DHA-deficient.
Fruit and vegetable intakes and the risk of colorectal cancer in the Breast Cancer Detection Demonstration Project follow-up cohort.

BACKGROUND Recent findings have cast doubt on the hypothesis that high intakes of fruit and vegetables are associated with a reduced risk of colorectal cancer. OBJECTIVE In a large prospective cohort of women, we examined the association between fruit and vegetable intakes and colorectal cancer. DESIGN Between 1987 and 1989, 45490 women with no history of colorectal cancer satisfactorily completed a 62-item Block-National Cancer Institute food-frequency questionnaire. During 386142 person-years of follow-up, 314 women reported incident colorectal cancer, searches of the National Death Index identified an additional 106 colorectal cancers, and a match with state registries identified another 65 colorectal cancers for a total of 485 cases. We used Cox proportional hazards regression analysis to estimate the relative risks (RRs) and 95% CIs in both energy-adjusted and fully adjusted models. RESULTS In models using the multivariate nutrient-density model of energy adjustment, RRs for increasing quintile of fruit consumption indicated no significant association with colorectal cancer [RR (95% CI)]: 1.00 (reference), 0.94 (0.70, 1.26), 0.85 (0.63, 1.15), 1.07 (0.81, 1.42), and 1.09 (0.82, 1.44). For vegetable consumption, there was also no significant association in the multivariate nutrient-density model with increasing quintiles of consumption: 1.00 (reference), 0.77 (0.58, 1.02), 0.83 (0.63, 1.10), 0.90 (0.69, 1.19), and 0.92 (0.70, 1.22). Additionally, 3 alternative models of energy adjustment showed no significant association between increases in vegetable intake and the risk of colorectal cancer. CONCLUSION Although the limitations of our study design and data merit consideration, this investigation provides little evidence of an association between fruit and vegetable intakes and colorectal cancer.
Molecular evidence and clinical importance of β‐arrestins expression in patients with acromegaly

β‐arrestins seem to have a role in endocytosis and desensitization of somatostatin receptor subtype 2 (sst2) and could be associated with the responsiveness to somatostatin receptor ligands (SRL) in patients with acromegaly. To investigate the in vivo correlation between β‐arrestins 1 and 2 with sst2, sst5 and dopamine receptor subtype 2 (D2) expressions, and the association of β‐arrestins with response to first‐generation SRL and invasiveness in somatotropinomas. β‐arrestins 1 and 2, sst2, sst5 and D2 mRNA expressions were evaluated by quantitative real‐time RT‐PCR on tumoral tissue of 96 patients. Moreover, sst2 and sst5 protein expressions were also evaluated in 40 somatotropinomas by immunohistochemistry. Response to SRL, defined as GH <1 μg/l and normal IGF‐I levels, was assessed in 40 patients. The Knosp‐Steiner criteria were used to define invasiveness. Median β‐arrestin 1, β‐arrestin 2, sst2, sst5 and D2 mRNA copy numbers were 478; 9375; 731; 156; and 3989, respectively. There was a positive correlation between β‐arrestins 1 and 2 (R = 0.444, P < 0.001). However, no correlation between β‐arrestins and sst2, sst5 (mRNA and protein levels) or D2 was found. No association was found between β‐arrestins expression and SRL responsiveness or tumour invasiveness. Although previous data suggest a putative correlation between β‐arrestins and sst2, our data clearly indicated that no association existed between β‐arrestins and sst2, sst5 or D2 expression, nor with response to SRL or tumour invasiveness. Therefore, further studies are required to clarify whether β‐arrestins have a role in the response to treatment with SRL in acromegaly.
Measuring the glycemic index of foods: interlaboratory study.

BACKGROUND Many laboratories offer glycemic index (GI) services. OBJECTIVE We assessed the performance of the method used to measure GI. DESIGN The GI of cheese-puffs and fruit-leather (centrally provided) was measured in 28 laboratories (n=311 subjects) by using the FAO/WHO method. The laboratories reported the results of their calculations and sent the raw data for recalculation centrally. RESULTS Values for the incremental area under the curve (AUC) reported by 54% of the laboratories differed from central calculations. Because of this and other differences in data analysis, 19% of reported food GI values differed by >5 units from those calculated centrally. GI values in individual subjects were unrelated to age, sex, ethnicity, body mass index, or AUC but were negatively related to within-individual variation (P=0.033) expressed as the CV of the AUC for repeated reference food tests (refCV). The between-laboratory GI values (mean+/-SD) for cheese-puffs and fruit-leather were 74.3+/-10.5 and 33.2+/-7.2, respectively. The mean laboratory GI was related to refCV (P=0.003) and the type of restrictions on alcohol consumption before the test (P=0.006, r2=0.509 for model). The within-laboratory SD of GI was related to refCV (P<0.001), the glucose analysis method (P=0.010), whether glucose measures were duplicated (P=0.008), and restrictions on dinner the night before (P=0.013, r2=0.810 for model). CONCLUSIONS The between-laboratory SD of the GI values is approximately 9. Standardized data analysis and low within-subject variation (refCV<30%) are required for accuracy. The results suggest that common misconceptions exist about which factors do and do not need to be controlled to improve precision. Controlled studies and cost-benefit analyses are needed to optimize GI methodology. The trial was registered at clinicaltrials.gov as NCT00260858.