Miracle-dz/UniFAR-FTUs huggingface dataset:
Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions

Abstract The effects of both bottom-up (e.g. substrate) and top-down (e.g. viral lysis) controls on the molecular composition of dissolved organic matter have not been investigated. In this study, we investigated the dissolved organic matter composition of the model bacterium Alteromonas macleodii ATCC 27126 growing on different substrates (glucose, laminarin, extracts from a Synechococcus culture, oligotrophic seawater, and eutrophic seawater), and infected with a lytic phage. The ultra-high resolution mass spectrometry analysis showed that when growing on different substrates Alteromonas macleodii preferred to use reduced, saturated nitrogen-containing molecules (i.e. O4 formula species) and released or preserved oxidized, unsaturated sulfur-containing molecules (i.e. O7 formula species). However, when infected with the lytic phage, Alteromonas macleodii produced organic molecules with higher hydrogen saturation, and more nitrogen- or sulfur-containing molecules. Our results demonstrate that bottom-up (i.e. varying substrates) and top-down (i.e. viral lysis) controls leave different molecular fingerprints in the produced dissolved organic matter.
Anti-Islanding Techniques for Integration of Inverter-Based Distributed Energy Resources to the Electric Power System

The emergence of microgrids and the increasing adoption of Distributed Generation Systems (DGS) have created an opportunity to replace traditional fossil fuels with renewable resources. Such a shift poses security and power quality challenges that must be addressed by academics and industrial research paradigms. Unintentional islanding is an important security concern, as it can result in power quality degradation, electrical hazards, and equipment damage. To address this problem and find efficient solutions, many anti-islanding techniques to detect and eliminate the phenomenon can be found in the specialized literature. These solutions can be classified as passive, active, remote, hybrid, or based on machine learning and signal processing techniques. In this context, this paper provides a comprehensive review of existing anti-islanding methods, highlighting their importance in preventing dangerous situations. The review includes a detailed analysis of the advantages and limitations found for each method, as well as its suitability for practical applications. The goal is to provide a valuable resource for researchers and practitioners in the field of distributed power systems, enabling them to choose the most appropriate anti-islanding method for their specific needs. Overall, this paper aims to address the challenges posed by unintentional islanding and promote the adoption of renewable energy resources for a more sustainable future.
Characterization and insight mechanism of an acid-adapted β-Glucosidase from Lactobacillus paracasei and its application in bioconversion of glycosides

Introduction: β-glucosidase is one class of pivotal glycosylhydrolase enzyme that can cleavage glucosidic bonds and transfer glycosyl group between the oxygen nucleophiles. Lactobacillus is the most abundant bacteria in the human gut. Identification and characterization of new β-glucosidases from Lactobacillus are meaningful for food or drug industry. Method: Herein, an acid-adapted β-glucosidase (LpBgla) was cloned and characterized from Lactobacillus paracasei. And the insight acid-adapted mechanism of LpBgla was investigated using molecular dynamics simulations. Results and Discussion: The recombinant LpBgla exhibited maximal activity at temperature of 30°C and pH 5.5, and the enzymatic activity was inhibited by Cu2+, Mn2+, Zn2+, Fe2+, Fe3+ and EDTA. The LpBgla showed a more stable structure, wider substrate-binding pocket and channel aisle, more hydrogen bonds and stronger molecular interaction with the substrate at pH 5.5 than pH 7.5. Five residues including Asp45, Leu60, Arg120, Lys153 and Arg164 might play a critical role in the acid-adapted mechanism of LpBgla. Moreover, LpBgla showed a broad substrate specificity and potential application in the bioconversion of glycosides, especially towards the arbutin. Our study greatly benefits for the development novel β-glucosidases from Lactobacillus, and for the biosynthesis of aglycones.
Biochemical Composition of Zooplankton of Khour Al-Zubair Port, Southern Iraq

The study of the biochemical composition of zooplankton, which constitutes an important part of the food chain, is an accurate criterion for the nutritional importance of these organisms, as they constitute the basic food for fish in their various stages of growth and other aquatic organisms of economic value. The present study aims to describe the significance of biochemical composition of marine zooplankton from Khour Al-Zubair. This study investigates the biochemical structure of zooplankton by studying their contents of proteins, lipids, ash, moisture and carbohydrates in the Iraqi marine waters (Khour Al-Zubair Port) during the period from August to December 2016. This has been accompanied by studying the water temperature and salinity. The results showed that the protein content of the zooplankton were 19.23 – 22.31 %, ash amounted to 3.86 – 4.45 %, while the lipids were 6.27 – 7.18 %, moisture were 65.82 –70.12 % and the carbohydrates 0.25 – 0.47 % wet weight
Selective Occupation by E2F and RB of Loci Expressed by RNA Polymerase III

Simple Summary RNA polymerase (pol) III synthesizes essential and abundant non-coding RNAs, including all tRNAs. This activity can be constrained by RB, an action that may help to limit cell growth and proliferation. Previous work established that RB binds and represses TFIIIB, a factor required for pol III to transcribe any of its target genes; this explains the apparently universal effect of RB on the pol III transcriptome. We find E2F bound to chromatin in close proximity to many pol III-transcribed genes, where it may recruit RB to influence local chromatin and control the production of specific products. RB recruitment to subsets of pol III-dependent genes provides the potential for more selective regulation than the general dampening of output that can be achieved through interaction with TFIIIB. The regulatory impact of E2F may therefore be strengthened by differentially influencing levels of key non-coding RNAs such as individual tRNAs. Abstract In all cases tested, TFIIIB is responsible for recruiting pol III to its genetic templates. In mammalian cells, RB binds TFIIIB and prevents its interactions with both promoter DNA and pol III, thereby suppressing transcription. As TFIIIB is not recruited to its target genes when bound by RB, the mechanism predicts that pol III-dependent templates will not be occupied by RB; this contrasts with the situation at most genes controlled by RB, where it can be tethered by promoter-bound sequence-specific DNA-binding factors such as E2F. Contrary to this prediction, however, ChIP-seq data reveal the presence of RB in multiple cell types and the related protein p130 at many loci that rely on pol III for their expression, including RMRP, RN7SL, and a variety of tRNA genes. The sets of genes targeted varies according to cell type and growth state. In such cases, recruitment of RB and p130 can be explained by binding of E2F1, E2F4 and/or E2F5. Genes transcribed by pol III had not previously been identified as common targets of E2F family members. The data provide evidence that E2F may allow for the selective regulation of specific non-coding RNAs by RB, in addition to its influence on overall pol III output through its interaction with TFIIIB.
Association of Longitudinal Changes in Cerebral Microstructure with Cognitive Functioning in Breast Cancer Survivors after Adjuvant Chemotherapy

Background: Adjuvant chemotherapy for breast cancer might impact cognitive function and brain structure. Methods: In this study, we investigated the cerebral microstructural changes in breast cancer survivors after adjuvant chemotherapy and the correlation with cognitive function with both cross-sectional and longitudinal study designs. All participants underwent structural MRI. In total, we recruited 67 prechemotherapy patients (BB), 67 postchemotherapy patients (BA), and 77 healthy controls (BH). For the follow-up study, 28 participants in the BH and 28 in the BB groups returned for imaging and assessment (BHF, BBF). Voxel-based morphometry analysis was performed to evaluate differences in brain volume; vertex-based shape analysis was used to assess the shape alterations of subcortical regions. Moreover, multiple regression was applied to assess the association between the changes in neuropsychological assessment and brain volume. Results: The results showed brain volume reduction in the temporal and parietal gyrus in BB and BA patients. Among each group, we also found significant shape alterations in the caudate and thalamus. Volume reductions in the temporal regions and shape changes in the caudate and hippocampus were also observed in patients from time point 1 to time point 2 (postchemotherapy). An association between brain volume and cognitive performance was also found in the limbic system. Conclusions: Based on our findings, we can provide a better understanding of the cerebral structural changes in breast cancer survivors, establish a subsequent prediction model, and serve as a reference for subsequent treatment.
Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts

Background: Small-diameter (<6 mm) artificial vascular grafts (AVGs) are urgently required in vessel reconstructive surgery but constrained by suboptimal hemocompatibility and the complexity of anastomotic procedures. This study introduces coaxial electrospinning and magnetic anastomosis techniques to improve graft performance. Methods: Bilayer poly(lactide-co-caprolactone) (PLCL) grafts were fabricated by coaxial electrospinning to encapsulate heparin in the inner layer for anticoagulation. Magnetic rings were embedded at both ends of the nanofiber conduit to construct a magnetic anastomosis small-diameter AVG. Material properties were characterized by micromorphology, fourier transform infrared (FTIR) spectra, mechanical tests, in vitro heparin release and hemocompatibility. In vivo performance was evaluated in a rabbit model of inferior vena cava replacement. Results: Coaxial electrospinning produced PLCL/heparin grafts with sustained heparin release, lower platelet adhesion, prolonged clotting times, higher Young’s modulus and tensile strength versus PLCL grafts. Magnetic anastomosis was significantly faster than suturing (3.65 ± 0.83 vs. 20.32 ± 3.45 min, p < 0.001) and with higher success rate (100% vs. 80%). Furthermore, magnetic AVG had higher short-term patency (2 days: 100% vs. 60%; 7 days: 40% vs. 0%) but similar long-term occlusion as sutured grafts. Conclusion: Coaxial electrospinning improved hemocompatibility and magnetic anastomosis enhanced implantability of small-diameter AVG. Short-term patency was excellent, but further optimization of anticoagulation is needed for long-term patency. This combinatorial approach holds promise for vascular graft engineering.
Development of an indoor positioning and navigation system using monocular SLAM and IMU

The positioning and navigation systems based on Global Positioning System (GPS) have been developed over past decades and have been widely used for outdoor environment. However, high-rise buildings or indoor environments can block the satellite signal. Therefore, many indoor positioning methods have been developed to respond to this issue. In addition to the distance measurements using sonar and laser sensors, this study aims to develop a method by integrating a monocular simultaneous localization and mapping (MonoSLAM) algorithm with an inertial measurement unit (IMU) to build an indoor positioning system. The MonoSLAM algorithm measures the distance (depth) between the image features and the camera. With the help of Extend Kalman Filter (EKF), MonoSLAM can provide real-time position, velocity and camera attitude in world frame. Since the feature points will not always appear and can't be trusted at any time, a wrong estimation of the features will cause the estimated position diverge. To overcome this problem, a multisensor fusion algorithm was applied in this study by using the multi-rate Kalman Filter. Finally, from the experiment results, the proposed system was verified to be able to improve the reliability and accuracy of the MonoSLAM by integrating the IMU measurements.
A Calculation Method for the Maximum Transmission Power in ICPT Systems Under Non-Sinusoidal Excitation

Calculating the maximum transmission power of loosely coupled coils represents a crucial step in the design of inductively coupled power transfer (ICPT) systems. However, the majority of calculations for the maximum power transfer point (MPTP) are exclusively relevant to scenarios featuring pure sinusoidal excitation. This paper introduces a more universally applicable solution for determining the MPTP in loosely coupled systems subjected to arbitrary excitations. The approach takes into account the contributions of both the fundamental waveform and all harmonics to the transmission capacity, offering a precise method for investigating the maximum power capacity of ICPT systems under non-sinusoidal excitation. Through simulations and experiments, it is demonstrated that the proposed method yields improved agreement with various waveform excitations, including square/triangular waves, as well as actual chopping waves characterized by large-scale ringing and overshoot. The versatility of the method extends to various inductively coupled systems with generic excitations, encompassing non-resonant-type, direct chopper, or multi-level inverter-driven ICPT systems. Furthermore, the method ensures greater accuracy in instances of excitation distortion induced by nonlinear effects.
The dynamics of vegetation change: health warnings for equilibrium 'dodo' models

Vegetation plays a part in controlling climate and in turn responds to climatic change. Therefore, projections of future climates must include the responses of vegetation, both in terms of function and distribution. Unfortunately, assessments of future climatic impacts on vegetation are still considered with equilibrium vegetation models-models which exclude processes involved in vegetation dynamics, such as succession and disturbance. The commonly held view that future spatial changes in temperature will outstrip the potential for species to migrate has no theoretical
Defining the threshold: triglyceride to high-density lipoprotein cholesterol (TG/HDL-C) ratio’s non-linear impact on tubular atrophy in primary membranous nephropathy

Background Hyperlipidemia is common in primary membranous nephropathy (PMN) patients, and tubular atrophy (TA) is an unfavorable prognostic factor. However, the correlation between the triglyceride to high-density lipoprotein cholesterol (TG/HDL-C) ratio and TA is controversial. Therefore, our study aimed to investigate the association between the TG/HDL-C ratio and TA in PMN patients. Methods We conducted a cross-sectional study and collected data from 363 PMN patients at Shenzhen Second People’s Hospital from January 2008 to April 2023. The primary objective was to evaluate the independent correlation between the TG/HDL-C ratio and TA using binary logistic regression model. We used a generalized additive model along with smooth curve fitting and multiple sensitivity analyses to explore the relationship between these variables. Additionally, subgroup analyses were conducted to delve deeper into the results. Results Of the 363 PMN patients, 75 had TA (20.66%). The study population had a mean age of 46.598 ± 14.462 years, with 217 (59.78%) being male. After adjusting for sex, age, BMI, hypertension, history of diabetes, smoking, alcohol consumption, UPRO, eGFR, HB, FPG, and ALB, we found that the TG/HDL-C ratio was an independent risk factor for TA in PMN patients (OR=1.29, 95% CI: 1.04, 1.61, P=0.0213). A non-linear correlation was observed between the TG/HDL-C ratio and TA, with an inflection point at 4.25. The odds ratios (OR) on the left and right sides of this inflection point were 1.56 (95% CI: 1.17, 2.07) and 0.25 (95% CI: 0.04, 1.54), respectively. Sensitivity analysis confirmed these results. Subgroup analysis showed a consistent association between the TG/HDL-C ratio and TA, implying that factors such as gender, BMI, age, UPRO, ALB, hypertension and severe nephrotic syndrome had negligible effects on the link between the TG/HDL-C ratio and TA. Conclusion Our study demonstrates a non-linear positive correlation between the TG/HDL-C ratio and the risk of TA in PMN patients, independent of other factors. Specifically, the association is more pronounced when the ratio falls below 4.25. Based on our findings, it would be advisable to decrease the TG/HDL-C ratio below the inflection point in PMN patients as part of treatment strategies.
The Role Of Financial Education To Financial Capability And Financial Management : Surakarta Students Analysis

This study aims to examine the influence of financial capabilities consisting of attitudes, behaviors, and knowledge on financial management mediated by financial education among students in Surakarta. The research method uses a quantitative approach with Structural Equation Modeling (SEM) Partial Least Square (PLS) analysis. Data was collected through an online survey by distributing a Google Form link. There were 170 respondents studied from various universities in Surakarta and most of them came from management and accounting departments. The results of the analysis show that financial education, financial skills consisting of attitudes, behavior and knowledge have a positive and significant effect on financial management, while financial education is successful in partially mediating the influence of attitudes, behavior and knowledge on students' financial management. The implication of these findings is that financial material has an influence on students in managing their finances, especially in terms of frugality and savings. Therefore, college student needs to know and apply the meaning of personal financial management to be able to become students who are good at managing finances and living more prosperously in the future.
Immunological Features of Children with Gastrointestinal Bleeding Due to Henoch-Schönlein Purpura

Background This study aims to evaluate the immunological features of gastrointestinal (GI) bleeding in children with Henoch-Schönlein purpura (HSP). Study Design This retrospective study was conducted on children with HSP. Demographic and clinical data were collected, including serum immunoglobulin (Ig) levels, complement C3 and C4 levels, and lymphocyte subtype percentage. Results A total of 446 hospitalized children had HSP. Eighty-six children with HSP had GI bleeding, 114 had proteinuria, and 107 had hematuria. Lower arthralgia, prolonged glucocorticoid use, increased white blood cell counts, elevated neutrophils and neutrophil-to-lymphocyte ratio, reduced IgG and C3 levels, elevated CD19+ cell percentage, and reduced CD3+ cell and natural killer cell percentages were associated with GI bleeding risk in patients with HSP. Multivariate regression analysis revealed that arthralgia, glucocorticoid use, increased neutrophil percentage, reduced IgG and C3 levels, and increased CD19+ cell percentage were independent predictors of GI bleeding. Further analysis indicated that the combination of C3 and CD19+ cell percentages had a high predictive ability for GI bleeding in children with HSP. Conclusion This study indicated that reduced C3 and increased CD19+ cell percentages contributed to the development of GI bleeding in children with HSP. Specific immunologic profiles may be strongly correlated with GI bleeding risk in children with HSP.
Development of Critical Thinking Skills Based Handout on the Topic of the Human Respiratory System to Improve Science Literacy in Junior High School Students

This research focuses on developing handout teaching materials based on critical thinking skills to enhance students' scientific literacy. The study employed the RD development method, along with the ADDIE model. The research subjects included material and media validators, science teachers, and eighth-grade of Junior high school students. The research utilized validation forms, questionnaire responses, pretest, and posttest as instruments. Data analysis techniques involved percentage equation, independent sample t-test, N-gain test, and effect size test. The results indicated that the handouts' quality, as assessed by material validators, was 92.31%, while media validators reported a score of 90.89%, placing both categories in the very decent range. There was a significant difference in average learning outcomes between students who utilize handouts and those who did not. The increase in scientific literacy ability was 0.7, indicating a high category, and the effect size of using handouts is 2.8, denoting a large category. Consequently, handout teaching materials based on critical thinking skills are deemed suitable for improving students' scientific literacy.
Assessment on accessibility of regional comprehensive transport:A case study of Anhui

The authors evaluate the provincial accessibility,county accessibility,opening accessibility between adjoining districts,two fare costs and probability of attraction based on social and economic factors of districts in Anhui Province,point out spatial accessibility structure of diversified indexes of towns in this province,and build a model of comprehensive accessibility indexes,and achieve spatial structure of comprehensive accessibility of the towns.We find that spatial pattern of the towns in Anhui is taking Hefei as the center and extending toward edge by and large,but the spatial pattern does not constitute regular concentric circles;county analysis reflects distinct differences between south towns and north towns.Accessibility of south and southwest towns is bad and good in the north towns,respectively;the towns are identified as severe shrinkage,sharp expansion,substantial stabilization and fluctuation in the opening analysis;spatial structure of fare cost based on the shortest line forms circularities centered embayment towards northeast;analysis of the least fare cost testifies spatial structure of accessibility of the towns following with extension of railway network;attractive indexes of probability indicate that the towns with high attractive indexes of probability present a shape of "? ";comprehensive accessibility indexes of the towns reveal symmetry between north and south in Anhui,but the pattern is broken by mountains of South Anhui.In the end,the researchers offer some proposals on some flaws of road network: promote new regional growth poles in north central Anhui for further strengthening their internal relations;accelerate the construction of high-grade highways in Huaibei plain,in particular strengthen lateral ties between the towns of North Jiangsu and South Henan provinces;extend connectivity of high grade roads in the northern and southern parts of Maanshan;improve the road facilities of the Wanjiang City Belt.