Miracle-dz/UniFAR-FTUs huggingface dataset:
Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

ABSTRACT This study seeks to assess the ability of seasonal trivalent inactivated influenza vaccine (TIV) to induce nonneutralizing antibodies (Abs) with Fc-mediated functions in HIV-uninfected and HIV-infected subjects. Functional influenza-specific Ab responses were studied in 30 HIV-negative and 27 HIV-positive subjects immunized against seasonal influenza. All 57 subjects received the 2015 TIV. Fc-mediated antihemagglutinin (anti-HA) Ab activity was measured in plasma before and 4 weeks after vaccination using Fc-receptor-binding assays, NK cell activation assays, and phagocytosis assays. At baseline, the HIV-positive group had detectable but reduced functional Ab responses to both vaccine and nonvaccine influenza antigens. TIV enhanced Fc-mediated Ab responses in both HIV-positive and HIV-negative groups. A larger rise was generally observed in the HIV-positive group, such that there was no difference in functional Ab responses between the two groups after vaccination. The 2015 TIV enhanced functional influenza-specific Ab responses in both HIV-negative and HIV-positive subjects to a range of influenza HA proteins. The increase in functional Ab responses in the HIV-positive group supports recommendations to immunize this at-risk group. IMPORTANCE Infection with HIV is associated with increasing disease severity following influenza infections, and annual influenza vaccinations are recommended for this target group. However, HIV-infected individuals respond relatively poorly to vaccination compared to healthy individuals, particularly if immunodeficient. There is therefore a need to increase our understanding of immunity to influenza in the context of underlying HIV infection. While antibodies can mediate direct virus neutralization, interactions with cellular Fc receptors may be important for anti-influenza immunity in vivo by facilitating antibody-dependent cellular cytotoxicity (ADCC) and/or antibody-dependent phagocytosis (ADP). The ability of seasonal influenza vaccines to induce antibody responses with potent Fc-mediated antiviral activity is currently unclear. Probing the ADCC and ADP responses to influenza vaccination has provided important new information in the quest to improve immunity to influenza.
Acral lentiginous melanoma mimicking benign disease: the Emory experience.

BACKGROUND Plantar and subungual melanoma exhibits a higher misdiagnosis rate relative to other anatomic sites. Misdiagnosis and delay in diagnosis are statistically associated with poorer patient outcome. Awareness of atypical presentations of acral melanoma may, thus, be important to decrease misdiagnosis rates and improve patient outcome. METHODS We conducted a retrospective case review of plantar or lower-extremity subungual melanoma performed at Winship Cancer Center, a tertiary care, referral center affiliated with Emory University, between 1985 and 2001. RESULTS A total of 53 cases of plantar or lower-extremity subungual melanoma were identified. Of 53 cases with a final diagnosis of melanoma, 18 were initially misdiagnosed. Misdiagnoses included wart, callous, fungal disorder, foreign body, crusty lesion, sweat gland condition, blister, nonhealing wound, mole, keratoacanthoma, subungual hematoma, onychomycosis, ingrown toenail, and defective/infected toenail. Of the 18 misdiagnosed cases, 9 were clinically amelanotic. CONCLUSION Awareness that amelanotic variants of acral melanoma may assume the morphology of benign hyperkeratotic dermatoses may increase the rate of correct diagnosis and improve patient outcome.
Polymorphisms in the IL6 gene in Asian Indian families with premature coronary artery disease – The Indian Atherosclerosis Research Study

Summary Inflammation plays a major role in coronary artery disease (CAD). We investigated the polymorphisms in the interleukin 6 (IL6) gene and their effect on the expression of acute-phase proteins in premature CAD in Asian Indian families. One hundred and ninety affected sibling pairs (ASPs) were genotyped for three tag single nucleotide polymorphisms (SNPs) in the IL6 gene for linkage analysis. We observed suggestive logarithm of odds (LOD) score for one SNP (rs2066992) in a subset of 62 ASPs with the age at onset less than 45 years (LOD score = 1.114, p = 0.011 in linkage analysis; pi = 0.55, p = 0.008 in identity by descent; LOD score = 1.06, p = 0.014 in quantitative trait locus for plasma levels of high sensitivity C-reactive protein, hsCRP). This was followed by sequencing of the promoter region and haplotype analysis in 46 probands and 40 controls. Five out of the eight previously reported promoter SNPs were found to be polymorphic (rs1800797, rs1800796, rs7802307, rs7802308, rs1800795). Two novel sequence variants were also found. One promoter haplotype (GGAAG) was detected with an odds ratio (OR) of 3.676 (p = 0.0017, 95% confidence interval [CI]: 1.68 – 8.045) and population attributable risk of 21.1% (95%CI: 9.2%-31.5%). The plasma levels of both hsCRP and fibrinogen exhibited significant association with these promoter SNP genotypes (p < 0.001). In conclusion, IL6 gene polymorphisms appear to be important genetic factors in premature CAD, and in the regulation of key atherogenic markers in Asian Indian families.
Schattauer GmbH Are Electronic Cardiac Devices Still Evolving ?

Objectives: The goal of this paper is to review some important issues occurring during the past year in Implantable devices. Methods: First cardiac implantable device was proposed to maintain an adequate heart rate, either because the heart’s natural pacemaker is not fast enough, or there is a block in the heart’s electrical conduction system. During the last forty years, pacemakers have evolved considerably and become programmable and allow to configure specific patient optimum pacing modes. Various technological aspects (electrodes, connectors, algorithms diagnosis, therapies, .) have been progressed and cardiac implants address several clinical applications: management of arrhythmias, cardioversion / defibrillation and cardiac resynchronization therapy. Results: Observed progress was the miniaturization of device, increased longevity, coupled with efficient pacing functions, multisite pacing modes, leadless pacing and also a better recognition of supraventricular or ventricular tachycardia’s in order to deliver appropriate therapy. Subcutaneous implant, new modes of stimulation (leadless implant or ultrasound lead), quadripolar lead and new sensor or new algorithm for the hemodynamic management are introduced and briefly described. Each times, the main result occurring during the two past years are underlined and repositioned from the history, remaining limitations are also addressed. Conclusion: Some important technological improvements were described. Nevertheless, news trends for the future are also considered in a specific session such as the remote follow-up of the patient or the treatment of heart failure by neuromodulation.
Presenting spatial tactile messages with a hand-held device

This paper introduces a multi-actuator tactile device designed for remote touch communication. While closely-spaced high-frequency vibrotactile actuators can be difficult to distinguish, our system utilized four linear DC motors for presenting spatial tactile messages through low-frequency actuation. An experiment was conducted to determine accuracy for recognizing stimuli presented on the palm of the hand. Participants were asked to identify 10 predefined stimulus patterns created from the four linear actuators positioned in either a diamond or square configuration. Results showed that positional, linear, and circular stimuli were recognized with mean response accuracies of 98.8, 96.5, and 90.2 %, respectively. No statistically significant differences were found between the actuator configurations. These findings can be utilized in developing a remote communication channel that supports the transfer of spatial aspects of touch such as mapping the location of finger touch of one user to tactile sensation on the palm of another user.
The fitness consequences of kin-biased dispersal in a cooperatively breeding bird

Cooperative alliances among kin may not only lead to indirect fitness benefits for group-living species, but can also provide direct benefits through access to mates or higher social rank. However, the immigrant sex in most species loses any potential benefits of living with kin unless immigrants disperse together or recruit relatives into the group in subsequent years. To look for evidence of small subgroups of related immigrants within social groups (kin substructure), we used microsatellites to assess relatedness between immigrant females of the cooperatively breeding superb starling, Lamprotornis superbus. We determined how timing of immigration led to kin subgroup formation and if being part of one influenced female fitness. Although mean relatedness in groups was higher for males than females, 26% of immigrant females were part of a kin subgroup with a sister. These immigrant sibships formed through kin recruitment across years more often than through coalitions immigrating together in the same year. Furthermore, females were more likely to breed when part of a kin subgroup than when alone, suggesting that female siblings form alliances that may positively influence their fitness. Ultimately, kin substructure should be considered when determining the role of relatedness in the evolution of animal societies.
On the use of a high-order finite element - wave based method for interior acoustic cavity analysis

This paper discusses the use of wave based prediction methods for the analysis of steady-state interior acoustic problems. Conventional element based prediction methods, such as the finite element method (FEM), are commonly used, but are restricted to low-frequency applications. The wave based method (WBM) is an alternative deterministic technique which is based on the indirect Trefftz approach. The WBM is computationally very efficient, allowing the analysis of problems at higher frequencies. The efficiency of the WBM is most pronounced for problems of moderate geometrical complexity. For the analysis of problems with a more complex geometry, a hybrid finite element-wave based method is developed. This hybrid approach combines the strengths of the two methods, namely, the high computational efficiency of the WBM and the ability of the FEM to model problems of arbitrary geometrical complexity. Up till now, only low-order FE models have been coupled with WB models. This paper discusses the application of more accurate high-order FE schemes in the hybrid FE-WB approach. The performance of the resulting high-order hybrid method will be illustrated by means of a numerical validation example.
Phased implementation of spaced clinic visits for stable HIV-positive patients in Rwanda to support Treat All

Introduction: In 2016, Rwanda implemented “Treat All,” requiring the national HIV programme to increase antiretroviral (ART) treatment coverage to all people living with HIV. Approximately half of the 164,262 patients on ART have been on treatment for more than five years, and long‐term retention of patients in care is an increasing concern. To address these challenges, the Ministry of Health has introduced a differentiated service delivery approach to reduce the frequency of clinical visits and medication dispensing for eligible patients. This article draws on key policy documents and the views of technical experts involved in policy development to describe the process of implementation of differentiated service delivery in Rwanda.
Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes

Synthetic lethal genetic interaction networks define genes that work together to control essential functions and have been studied extensively in Saccharomyces cerevisiae using the synthetic genetic array (SGA) analysis technique (ScSGA). The extent to which synthetic lethal or other genetic interaction networks are conserved between species remains uncertain. To address this question, we compared literature-curated and experimentally derived genetic interaction networks for two distantly related yeasts, Schizosaccharomyces pombe and S. cerevisiae. We find that 23% of interactions in a novel, high-quality S. pombe literature-curated network are conserved in the existing S. cerevisiae network. Next, we developed a method, called S. pombe SGA analysis (SpSGA), enabling rapid, high-throughput isolation of genetic interactions in this species. Direct comparison by SpSGA and ScSGA of ∼220 genes involved in DNA replication, the DNA damage response, chromatin remodeling, intracellular transport, and other processes revealed that ∼29% of genetic interactions are common to both species, with the remainder exhibiting unique, species-specific patterns of genetic connectivity. We define a conserved yeast network (CYN) composed of 106 genes and 144 interactions and suggest that this network may help understand the shared biology of diverse eukaryotic species.
EXPLORING THE POTENTIAL OF MULTIVARIATE ANALYSIS TO STUDY THE IMPACT OF COTTON LEAF CURL DISEASE ON YIELD TRAITS

Cotton production in Pakistan seems adequate, but inclined to several biotic and abiotic constraints, especially Cotton Leaf Curl Virus posing a hazardous impact on cotton yield by affecting yield traits, fiber quality and health status of plants. In this study, eight Bt and four Non-Bt cotton genotypes were sown under augmented design in the experimental area of the Department of Plant Pathology, University of Agriculture, Faisalabad. Presence of virus was confirmed by using the graft inoculation technique in greenhouse while natural inoculum was also relied upon for infection under field conditions. Data encompassing plant height, number of bolls, 50 % open bolls, boll weight, number of sympodial and monopodial branches were collected after disease appearance and subjected to multivariate analyses of variance which revealed that all genotypes responded distinctly to Cotton Leaf Curl Disease. Then these data were subjected to linear discriminant analysis where first two linear discriminants explained 61.05% and 23.54% variability respectively, and highlighted plant height and 50% open bolls as maximum variability explaining cotton yield traits in response to disease. Territorial map was constructed between first two linear discriminants which revealed that disease influenced plant height and 50% open bolls of Bt cotton genotypes potentially than the Non-Bt genotypes. Moreover, data for plant height and 50% open bolls were split and subjected to Hypothesis-error (HE) plot which depicted negative correlation between plant height and 50% open bolls and described the results in conformation with territorial map. Hence, these tools can be potentially used to study the impact of disease on crop yield traits and for selection of lines/varieties either for future breeding program or farmer field.
Conditional Reasoning with Subjective Logic

Conditional inference plays a central role in logical and Bayesian reasoning, and is used in a wide range of applications. It basically consists of expressing conditional relationship between parent and child propositions, and then to combine those conditionals with evidence about the parent propositions in order to infer conclusions about the child propositions. While conditional reasoning is a well established part of classical binary logic and probability calculus, its extension to belief theory has only recently been proposed. Subjective opinions represent a special type of general belief functions. This article focuses on conditional reasoning in subjective logic where beliefs are represented in the form of binomial or multinomial subjective opinions. Binomial conditional reasoning operators for subjective logic have been defined in previous contributions. We extend this approach to multinomial opinions, thereby making it possible to represent conditional and evidence opinions on frames of arbitrary size. This makes subjective logic a powerful tool for conditional reasoning in situations involving ignorance and partial information, and makes it possible to analyse Bayesian network models with uncertain probabilities.
Diversities of interaction of murine macrophages with three strains of Candida albicans represented by MyD88, CARD9 gene expressions and ROS, IL-10 and TNF-α secretion.

AIM To explore the mechanisms underlying the different responses of macrophages to distinct Candida albicans strains. METHODS Bone marrow was collected from mice. Macrophages were independently incubated with 3 Candida albicans strains. RESULTS MyD88 expression in Candida albicans 3683 group was significantly higher than that in Candida albicans 3630 group and Candida albicans SC5314 group, and marked difference was also observed between later two groups (P<0.05). CARD9 expression in Candida albicans 3630 group was higher than that in Candida albicans 3683 group and Candida albicans SC5314 group. Fluorescence intensity was 46.78±0.79 in Candida albicans 3630 group, 32.60±1.31 in Candida albicans 3683 group and 19.40±0.58 in Candida albicans SC5314, and significant difference was observed between any two groups (P<0.05). TNF-α and IL-10 were 18.9843±0.7081 pg/ml and 11.6690±0.3167 pg/ml, respectively, in Candida albicans 3683 group, which were markedly higher than those in Candida albicans 3630 group and Candida albicans SC5314 group (P<0.05 and 0.01). CONCLUSION Different Candida albicans strains may induce CARD9 expression and alter the production of ROS, TNF-α and IL-10 in macrophages, which may be one of mechanisms underlying the different killing effects of macrophages on distinct Candida albicans strains.
A molecular engineering toolbox for the structural biologist

Abstract Exciting new technological developments have pushed the boundaries of structural biology, and have enabled studies of biological macromolecules and assemblies that would have been unthinkable not long ago. Yet, the enhanced capabilities of structural biologists to pry into the complex molecular world have also placed new demands on the abilities of protein engineers to reproduce this complexity into the test tube. With this challenge in mind, we review the contents of the modern molecular engineering toolbox that allow the manipulation of proteins in a site-specific and chemically well-defined fashion. Thus, we cover concepts related to the modification of cysteines and other natural amino acids, native chemical ligation, intein and sortase-based approaches, amber suppression, as well as chemical and enzymatic bio-conjugation strategies. We also describe how these tools can be used to aid methodology development in X-ray crystallography, nuclear magnetic resonance, cryo-electron microscopy and in the studies of dynamic interactions. It is our hope that this monograph will inspire structural biologists and protein engineers alike to apply these tools to novel systems, and to enhance and broaden their scope to meet the outstanding challenges in understanding the molecular basis of cellular processes and disease.
Columnar modelling of nucleation burst evolution in the convective boundary layer – first results from a feasibility study Part II: Meteorological characterisation

While in Paper I of four papers a revised columnar high-order modelling approach to investigate gas-aerosol-turbulence interactions in the convective boundary layer (CBL) was deduced, in the present Paper II the model capability to predict the evolution of meteorological CBL parameters is demonstrated. Based on a model setup to simulate typical CBL conditions, predicted first-, second- and third-order moments were shown to agree very well with those obtained from in situ and remote sensing turbulence measurements such as aircraft, SODAR and LIDAR measurements as well as with those derived from ensemble-averaged large eddy simulations and wind tunnel experiments. The results show, that the model is able to predict the meteorological CBL parameters, required to verify or falsify, respectively, previous hypothesis on the interaction between CBL turbulence and new particle formation.
Active and passive distraction using a head-mounted display helmet: effects on cold pressor pain in children.

OBJECTIVE The current study tested the effectiveness of interactive versus passive distraction that was delivered via a virtual reality type head-mounted display helmet for children experiencing cold pressor pain. DESIGN Forty children, aged 5 to 13 years, underwent 1 or 2 baseline cold pressor trials followed by interactive distraction and passive distraction trials in counterbalanced order. MAIN OUTCOME MEASURES Pain threshold and pain tolerance. RESULTS Children who experienced either passive or interactive distraction demonstrated significant improvements in both pain tolerance and pain threshold relative to their baseline scores. In contrast, children who underwent a second cold pressor trial without distraction showed no significant improvements in pain tolerance or threshold. CONCLUSION Although both distraction conditions were effective, the interactive distraction condition was significantly more effective. Implications for the treatment of children's distress during painful medical procedures are discussed.
Breast cancer in pregnancy: a literature review.

HYPOTHESIS Breast cancer in pregnancy will increase as more women postpone childbearing until later in life. OBJECTIVE To review the literature on diagnosis, staging, treatment, and prognosis. DESIGN AND METHODS Articles were obtained from MEDLINE (1966-present) using the keywords breast, cancer, carcinoma, and pregnancy. Additional articles were sought using the references of those obtained. A total of 171 articles were found, 125 in English. More than 100 were reviewed, including 7 prospective and 40 retrospective studies, 6 case reports, and at least 47 review articles on various aspects of pregnancy and cancer. Data extraction was performed by 1 reviewer. RESULTS Diagnostic delays are shorter than in the past but remain common. Mammography has a high false-negative rate during pregnancy. Biopsy or needle aspiration are needed for diagnosis and cannot be postponed until after delivery. Pregnancy-associated cancers tend to occur at a later stage and be estrogen receptor-negative. However, they carry a similar prognosis to other breast cancers when matched for stage and age. Although modified radical mastectomy is the traditional treatment, breast-conserving therapy is increasingly common. Therapeutic radiation is contraindicated, but chemotherapy is relatively safe after the first trimester. Tamoxifen should be avoided in the first trimester and possibly beyond. CONCLUSIONS Physicians should perform a thorough breast examination at the first prenatal visit and maintain a high index of suspicion for cancer. Patients who wish to continue their pregnancies have a growing array of treatment options.
Eyes-free multitasking: the effect of cognitive load on mobile spatial audio interfaces

As mobile devices increase in functionality, users perform more tasks when on the move. Spatial audio interfaces offer a solution for eyes-free interaction. However, such interfaces face a number of challenges when supporting multiple and simultaneous tasks, namely: 1) interference amongst multiple audio streams, and 2) the constraints of cognitive load. We present a comparative study of spatial audio techniques evaluated in a divided- and selective-attention task. A podcast was used for high cognitive load (divided-attention) and classical music for low cognitive load (selective-attention), while interacting with an audio menu. Results showed that spatial audio techniques were preferred when cognitive load was kept low, while a baseline technique using an interruptible single audio stream was significantly less preferred. Conversely, when cognitive load was increased the preferences reversed. Thus, given an appropriate task structure, spatial techniques offer a means of designing effective audio interfaces to support eyes-free mobile multitasking.