Miracle-dz/UniFAR-FTUs huggingface dataset:
False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV

ABSTRACT To evaluate the reactivity of the recombinant proteins expressed in Escherichia coli strain BL21(DE3), a Western blot assay was performed by using a panel of 78 serum samples obtained, respectively, from convalescent-phase patients infected with severe acute respiratory syndrome-associated coronavirus (SARS-CoV) (30 samples) and from healthy donors (48 samples). As antigen for detection of SARS-CoV, the nucleocapsid protein (N) showed high sensitivity and strong reactivity with all samples from SARS-CoV patients and cross-reacted with all serum samples from healthy subjects, with either those obtained from China (10 samples) or those obtained from France (38 serum samples), giving then a significant rate of false positives. Specifically, our data indicated that the two subunits, S1 (residues 14 to 760) and S2 (residues 761 to 1190), resulted from the divided spike reacted with all samples from SARS-CoV patients and without any cross-reactivity with any of the healthy serum samples. Consequently, these data revealed the nonspecific nature of N protein in serodiagnosis of SARS-CoV compared with the S1 and S2, where the specificity is of 100%. Moreover, the reported results indicated that the use of one single protein as a detection antigen of SARS-CoV infection may lead to false-positive diagnosis. These may be rectified by using more than one protein for the serodiagnosis of SARS-CoV.
The 2008 Mumbai terror attacks: (re-)constructing Indian (counter-)terrorism

This paper deploys a discursive approach to the ‘scripting’ of the November 2008 Mumbai ‘terror attacks’ and their aftermath, including ensuing debates about counter-terrorism in India. It explores the perspectives of a range of actors who participated in very different ways in the social construction of the attacks, including media consumers and producers, key participants in public debates, and even the masterminds of the attacks. Important insights into the planning and implementation of the attacks are drawn from phone conversations intercepted by Indian intelligence, between the gunmen and their ‘controllers’. The Mumbai attacks were scripted and staged in a conscious effort to obtain maximum media coverage, which also made the masterminds dependent on the media. The war story created by the media featured violence simply as a means of ‘fighting a battle’, obscuring the significant role of violence as a display of force by both security forces and ‘terrorists’.
Immunohistochemical localization by monoclonal antibody of human epidermal growth factor in mixed tumours of the skin

Immunohistochemical distribution of human epidermal growth factor (hEGF) was described in 17 cases of mixed tumour of the skin with monoclonal antibody. In normal sweat glands, epithelial cells in the secretory portion and in the transitional area between secretory portion and duct showed prominent staining for hEGF. In the salivary pleomorphic adenoma type of mixed tumour of the skin, luminal tumour cells of tubular and duct‐like structures gave a very characteristic hEGF staining reaction. The tumour cells showing strong staining for hEGF were scattered throughout the solid foci in this type of mixed tumour. Tubular epithelial cells in the clear cell adenoma type also displayed a positive hEGF reaction. And apocrine mixed tumours strong staining for hEGF occurred on the apical side of tubular and ductal tumour cells. In view of the immunohistochemical staining patterns for hEGF, the histologic origin of mixed tumours of the skin is suggested to be cells in the secretory portion and (hose in the transitional portion between secretory portion and duct of the sweat gland.
Functional somato‐dendritic α7‐containing nicotinic acetylcholine receptors in the rat basolateral amygdala complex

Multiple subtypes of nicotinic acetylcholine receptors (nAChRs) are expressed in the CNS. The amygdala complex, the limbic structure important for emotional memory formation, receives cholinergic innervation from the basal forebrain. Although cholinergic drugs have been shown to regulate passive avoidance performance via the amygdala, the neuronal subtypes and circuits involved in this regulation are unknown. In the present study, whole‐cell patch‐clamp electrophysiological techniques were used to identify and characterize the presence of functional somato‐dendritic nAChRs within the basolateral complex of the amygdala. Pressure‐application of acetylcholine (ACh; 2 mm) evoked inward current responses in a subset of neurons from both the lateral (49%) and basolateral nuclei (72%). All responses displayed rapid activation kinetics, and were blocked by the α7‐selective antagonist methyllycaconitine. In addition, the α7‐selective agonist choline induced inward current responses that were similar to ACh‐evoked responses. Spiking patterns were consistent with pyramidal class I neurons (the major neuronal type in the basolateral complex); however, there was no correlation between firing frequency and the response to ACh. The local photolysis of caged carbachol demonstrated that the functional expression of nAChRs is located both on the soma and dendrites. This is the first report demonstrating the presence of functional nAChR‐mediated current responses from rat amygdala slices, where they may be playing a significant role in fear and aversively motivated memory.
Overview: Governance in higher education—concepts and issues

This paper presents an overview of key concepts and issues in the governance of higher education. It is written both as an introduction to and as a reflection upon the collection of papers presented in this Special Issue of Higher Education: Research & Development. It seeks to locate important clusters of research interest relating to higher education governance, and it advances a perspective on the topic that might assist in guiding and encouraging further research.
Prosuming Multiple Gender Role Identities: 1 A Multi‐country Written and Audio‐visual Exploration of Contemporary Young Mainstream Female Achievers

Our research focuses on contemporary young mainstream female achievers (CYMFAs) living in affluent middle class milieus. Motivation to study this cohort originates from our contention that compared to other women in recorded history CYMFAs have the greatest opportunity to strongly determine their gender roles, lifestyles and consumption activities. An important finding, amongst others, is that CYMFAs may (un)consciously prosume multiple gender role identities so as to operate effectively in a wide variety of everyday gender role domains. We present our research in two complementary parts. Our journal article details the intellectual foundation of the inquiry, research methods and importantly in‐depth findings derived from across‐case analysis. The film supplements the written paper by presenting videographies of each informant. The videographies make generous use of raw footage of informants' interviews, their daily activities, re‐enactments, visual metaphors and lyrical music. In adopting this approach, we strive to increase the transferability of our written findings by providing a rich context, via film, for their interpretation.
Identifiability of linear lossless Port-controlled Hamiltonian systems

The aim of this paper, is to study the identifiability property, of Port-Controlled Hamiltonian systems. A simple identifiability condition, is derived by transforming the port-Hamiltonian systems to the observable canonical form. Indeed, the observable canonical form, gives the possibility to represent the transfer function in a simplified form, similar to ARMAX models, used in identification. In order to test the identifiability of Port-Controlled Hamiltonian systems, a test was realized in the case of a LC circuit.
Brazilian recommendations of mechanical ventilation 2013. Part I

Perspectives on invasive and noninvasive ventilatory support for critically ill patients are evolving, as much evidence indicates that ventilation may have positive effects on patient survival and the quality of the care provided in intensive care units in Brazil. For those reasons, the Brazilian Association of Intensive Care Medicine (Associação de Medicina Intensiva Brasileira - AMIB) and the Brazilian Thoracic Society (Sociedade Brasileira de Pneumologia e Tisiologia - SBPT), represented by the Mechanical Ventilation Committee and the Commission of Intensive Therapy, respectively, decided to review the literature and draft recommendations for mechanical ventilation with the goal of creating a document for bedside guidance as to the best practices on mechanical ventilation available to their members. The document was based on the available evidence regarding 29 subtopics selected as the most relevant for the subject of interest. The project was developed in several stages, during which the selected topics were distributed among experts recommended by both societies with recent publications on the subject of interest and/or significant teaching and research activity in the field of mechanical ventilation in Brazil. The experts were divided into pairs that were charged with performing a thorough review of the international literature on each topic. All the experts met at the Forum on Mechanical Ventilation, which was held at the headquarters of AMIB in São Paulo on August 3 and 4, 2013, to collaboratively draft the final text corresponding to each sub-topic, which was presented to, appraised, discussed and approved in a plenary session that included all 58 participants and aimed to create the final document.
Wi-Fi/WiMAX dual mode RF MMIC front-end module

A dual-mode RF front-end module is designed and implemented for Wi-Fi/WiMAX applications. It consists of a front-end MMIC and a dual-band power amplifier MMIC, both fabricated by 0. 5μm E/D-mode p-HEMT process. The front-end MMIC integrates a single-pole triple-throw antenna switch, two low noise amplifiers, a low pass filter and a diplexer in single chip. Overall module size is compact 7 mm × 10 mm, well tested with Wi-Fi/WiMAX OFDM signals.
Understanding challenges and opportunities of preventive blood pressure self-monitoring at home

The herein presented study explores socio-technical complexities that may occur when introducing preventive health-measurement technologies in older adults' daily routines and everyday lives. Using mainly blood pressure measurements in private homes, the study applied qualitative and quantitative methods to understand existing challenges and uncover opportunities of self-monitoring technologies to support preventive healthcare activities among older adults. Emerging challenges from our study were: rule complexity for self-measuring, reliability of measurements, interpretation, understanding and health awareness, the sharing activity for prevention, motivational sources, the role of the doctor for prevention, older adult's use of technology, the home as a distributed information space, and visualization of health data. An awareness of these challenges can help designers to develop better tools to support people's preventive self-monitoring needs compared with existing solutions. Furthermore, supporting the active and informed citizen can improve older adult's care abilities, awareness and activation towards preventive care.
Distractors and Distressors in Involuntary Status Decision-Making

This article examines a series of emotive and judgmental descriptors that from time to time are availed of in the context of civil commitment of patients by both clinicians and mental health review tribunals. Those which are scrutinised are: 'insightless', 'non-compliant', 'promiscuous', 'absconder', 'violent', 'substance dependent', 'disorganised', 'poor hygiene', 'impaired judgment' and 'personality disordered'. Decisions of the Victorian Mental Health Review Board are used to illustrate the issues. The argument is put that it is necessary for clinicians and review tribunals alike to 'unpack' such descriptors, use them with great care, always assess whether they are justified by reliable, contemporary evidence, probe their contexts, and decide whether they are the product of subjective judgments which may have alternative explanations.
Paricalcitol capsule for the treatment of secondary hyperparathyroidism in stages 3 and 4 CKD.

BACKGROUND The safety and efficacy of paricalcitol injection have been well established for the prevention and treatment of secondary hyperparathyroidism (SHPT) in patients with chronic kidney disease (CKD) stage 5. The capsule form of paricalcitol was developed to provide a convenient dosage form for patients with stages 3 and 4 CKD. METHODS Three randomized, placebo-controlled, phase-3 trials were conducted in patients with stages 3 and 4 CKD with SHPT. Enrollment criteria included an estimated glomerular filtration rate between 15 and 60 mL/min/1.73 m2 (0.25 and 1.00 mL/s/1.73 m2), an average of 2 consecutive intact parathyroid hormone (iPTH) levels greater than 150 pg/mL (ng/L), 2 consecutive serum calcium levels between 8.0 and 10.0 mg/dL (2.00 and 2.50 mmol/L), and 2 consecutive serum phosphorus levels of 5.2 mg/dL or less (< or = 1.68 mmol/L). Two studies used a thrice-weekly dosing regimen and 1 study used a once-daily dosing regimen for 24 weeks. Dosing was based on serum iPTH, calcium, and phosphorus levels. The primary efficacy end point is 2 consecutive decreases in iPTH levels greater than 30% from baseline. RESULTS Two hundred twenty patients participated (n = 107, paricalcitol; n = 113, placebo). At least 2 consecutive decreases in iPTH levels of 30% or greater from baseline occurred in 91% of paricalcitol versus 13% of placebo patients (P < 0.001). Incidences of hypercalcemia, hyperphosphatemia, and elevated calcium-phosphorus product levels were not significantly different between groups. Similarly, no significant differences in urinary calcium and phosphorus excretion or deterioration in kidney function were detected in patients administered paricalcitol compared with placebo. CONCLUSION Paricalcitol capsule was well tolerated and effectively decreased iPTH levels with minimal or no impact on calcium levels, phosphorus balance, and kidney function in patients with stages 3 and 4 CKD.
Virtual reality rehabilitation system for neuropathic pain and motor dysfunction in spinal cord injury patients

Spinal cord injury (SCI) causes both lower limb motor dysfunction and associated neuropathic pain. Although these two conditions share related cortical mechanisms, different interventions are currently used to treat each condition. With intensive training using entertaining virtual reality (VR) scenarios, it may be possible to reshape cortical networks thereby reducing neuropathic pain and improving motor function. We have created the first VR training system combining action observation and execution addressing lower limb function in incomplete SCI (iSCI) patients. A particular feature of the system is the use of size-adjustable shoes with integrated motion sensors. A pilot single-case clinical study is currently being conducted on six iSCI patients. Two patients tested to date were highly motivated to perform and reported improved physical well-being. They improved in playing skill and in controlling the virtual lower limbs. There were post-intervention indications of neuropathic pain decrease, muscle strength increase, faster walking speed and improved performance on items relevant for ambulation. In addition functional MRI before and after treatment revealed a decreased activation pattern. We interpret this result as an improvement of neuronal synergies for this task. These results suggest that our VR system may be beneficial for both reducing neuropathic pain and improving motor function in iSCI patients.
De la crise politique aux échecs des projets d'investissements agricoles à grande échelle

L'" affaire Daewoo ", immense projet d'investissements agricoles de l'entreprise sud-coreenne Daewoo Logistics a Madagascar, marque le debut de la crise politique de decembre 2008. Comment la crise a-t-elle joue sur les dynamiques d'investissements et d'appropriation a grande echelle des terres de la Grande Ile ? (Resume d'auteur)
Communication Patterns of Managers

A "self-report" communication diary was maintained for a period of one working week by 101 managers in a variety of Australian public and private sector organizations. The results confirm prior research and add substantially to our understanding of the relationship between key demo graphic variables, e.g., organizational function, education, public vs. private sector; and selected communication characteristics, e.g., numbers and types present, use of face-to-face vs. interposed media, time spent on work vs. nonwork activities. These findings have important implications for the way managers use their time and are trained.
Serum Pyridinoline Levels and Prediction of Severity of Joint Destruction in Rheumatoid Arthritis

Objective. Previous studies indicated that pyridinoline, a collagen crosslink in cartilage and bone, might be a good marker to predict joint destruction in patients with rheumatoid arthritis (RA), although large prospective studies are lacking. We evaluated the predictive value of serum pyridinoline levels for joint destruction, both at baseline for longterm prediction and during the disease course for near-term prediction. Methods. Patients with early RA from the Leiden Early Arthritis Clinic were studied. Radiographs at baseline and yearly during 7 years of followup were scored according to the Sharp-van der Heijde Scoring (SHS) method. Pyridinoline serum levels at baseline and during followup were measured by ELISA. The association between baseline pyridinoline levels and difference in SHS over 7 years was tested, with a multivariate normal regression model. Second, the association between pyridinoline levels determined during the disease course and progression of SHS over the next year was tested with a multivariable linear regression analysis. Results. Studying baseline pyridinoline serum levels in 437 patients revealed that the mean SHS over 7 years was 6% higher for every higher pyridinoline level (nmol/l) at baseline (p = 0.001). Subsequently, during followup (n = 184 patients) the progression in SHS in the upcoming year was 17% higher for every higher nmol/l pyridinoline level (p = 0.001). The area under the receiver-operation characteristic curve for rapid radiological progression was 0.59. Conclusion. Increased pyridinoline serum levels, both at baseline and during the disease course, are associated with more severe joint destruction during the coming year(s), although the predictive accuracy as a sole predictor was moderate.
Using Social Space Models for Inference on Missing Nodes

Social network analysis generally assumes a model for the network giving the probabilistic edge structure of the graph. One class of models posits a “social space”, defined by latent variables, where the position of the vertices in the space determines the edge probabilities. This has been used for various inferences including predicting missing edges and assigning labels to unlabeled vertices. Often one assumes that all the vertices have been observed, or that the only vertices about which information is sought are those that have been observed. It may seem that the very nature of these models make it impossible to make inferences about missing vertices. We discuss a method for making these inferences, under certain assumptions about the social space, and give some examples of this inference using a simple model which can be fit using a least squares algorithm.
Structural basis of the phosphorylation-independent recognition of cyclin D1 by the SCFFBXO31 ubiquitin ligase

Significance The cyclin D1 proto-oncoprotein is a crucial regulator of cell cycle progression, and excessive cyclin D1 expression and/or activity is a hallmark of many human cancers. The SCFFBXO31 ubiquitin ligase complex is responsible for targeting cyclin D1 for ubiquitination and degradation, thereby regulating its turnover after DNA damage. In this work, we report the structural, biophysical, and functional characterization of the interaction between cyclin D1 and Skp1–FBXO31. We find that the F-box protein FBXO31 targets cyclin D1 for phosphorylation-independent ubiquitination by recognizing and binding to its extreme C terminus. This knowledge enhances our understanding of the molecular mechanism of FBXO31-mediated cyclin D1 proteolysis following genotoxic stress and may facilitate efforts to identify a novel molecular target for anticancer drug discovery. Ubiquitin-dependent proteolysis of cyclin D1 is associated with normal and tumor cell proliferation and survival. The SCFFBXO31 (Skp1–Cul1–Rbx1–FBXO31) ubiquitin ligase complex mediates genotoxic stress-induced cyclin D1 degradation. Previous studies have suggested that cyclin D1 levels are maintained at steady state by phosphorylation-dependent nuclear export and subsequent proteolysis in the cytoplasm. Here we present the crystal structures of the Skp1–FBXO31 complex alone and bound to a phosphorylated cyclin D1 C-terminal peptide. FBXO31 possesses a unique substrate-binding domain consisting of two β-barrel motifs, whereas cyclin D1 binds to FBXO31 by tucking its free C-terminal carboxylate tail into an open cavity of the C-terminal FBXO31 β-barrel. Biophysical and functional studies demonstrate that SCFFBXO31 is capable of recruiting and ubiquitinating cyclin D1 in a phosphorylation-independent manner. Our findings provide a conceptual framework for understanding the substrate specificity of the F-box protein FBXO31 and the mechanism of FBXO31-regulated cyclin D1 protein turnover.
Chronic Procrastination Among Turkish Adults: Exploring Decisional, Avoidant, and Arousal Styles

The authors examined the prevalence of chronic procrastination (decisional, avoidant, and arousal styles) as functions of demographic variables among 354 Turkish adults (148 men, 206 women; M age = 38.7 years, SD = 8.26 years). Prevalence analyses showed that among Turkish participants, 17.5% were indecisive procrastinators, 13.8% were avoidant procrastinators, and 14.7% were arousal procrastinators. Results did not yield significant differences for gender or age on any forms of procrastination, which is consistent with other international samples. However, significant differences emerged depending on the number of children such that Turkish adults who had more than 3 children claimed to be more indecisive than they claimed to be arousal or avoidant procrastinators. Respondents with less than a graduate degree reported higher rates of indecision than did respondents with at least a graduate degree.