Miracle-dz/UniFAR-FTUs huggingface dataset:
Local kinematics and the local standard of rest

We re-examine the stellar kinematics of the solar neighbourhood in terms of the velocity υ� of the Sun with respect to the local standard of rest. We show that the classical determination of its component Vin the direction of Galactic rotation via Str¨ omberg's relation is undermined by the metallicity gradient in the disc, which introduces a correlation between the colour of a group of stars and the radial gradients of its properties. Comparing the local stellar kinematics to a chemodynamical model which accounts for these effects, we obtain (U, V, W)� = (11.1 +0.69 −0.75 , 12.24 +0.47 −0.47 ,7 .25 +0.37 −0.36 )k m s −1 , with additional systematic uncertainties ∼(1, 2, 0.5) km s −1 . In particular, Vis 7 km s −1 larger than previously estimated. The new values of (U, V, W)� are extremely insensitive to the metallicity gradient within the disc.
Motivating Weight Loss Among Black Adults in Relationships: Recommendations for Weight Loss Interventions

Background Black men and women have the highest rates of obesity in the United States. Behavioral weight loss programs incorporating intimate partners may be effective in combating obesity among this population. Yet, current participation in these programs is low. Identifying motivations and triggers to weight loss may provide insight in designing programs to increase participation. Aim To determine triggers and motivations for weight loss among Black men and women in committed relationships to inform development of weight loss interventions. Method Twenty semi-structured interviews, based on an integrated theoretical framework of interdependence and communal coping and the social cognitive theory, were conducted among Black heterosexual couples where one or both individuals intentionally lost ≥5% of their body weight in the last 6 months in a metropolitan region in a southern state. Interviews were transcribed and coded. Themes were identified following multi-rater coding and direct and conventional content analysis. Recommendations were developed from emergent themes. Results Among individuals with recent weight loss, personal relevancy and awareness (health awareness and self-awareness) were identified as personal triggers for weight loss initiation. Health concerns and appearance were general motivations among the total sample. Conclusion Emphasizing the impact of weight on daily functioning and quality of life, and increasing awareness of personal weight status and health consequences, may increase weight loss initiation and participation in weight loss programs among Black men and women in committed relationships. Findings also highlight strategies to improve recruitment and retention and guide intervention development and implementation for this population.
Robot‐assisted laparoscopic surgery with a fluorescent near‐infrared ray ureteral catheter for a rectal cancer patient with pelvic kidney: A case report

An 85‐year‐old woman presented with a stomachache after a meal and was admitted to the previous clinic. Multi‐detector computed tomography (CT) of the abdomen showed wall thickening in the rectum and right ectopic pelvic kidney. Colonoscopy revealed a mass at the rectum, and a biopsy showed adenocarcinoma. CT showed no lymphadenopathy or distant metastasis. Hartmann's procedure with fluorescent near‐infrared ray ureteral catheters was used to avoid causing urinary injury. Robotic surgery was performed while checking the route of the ureter in near‐infrared mode. The patient was discharged on postoperative day 14 without specific complications. This case appears to be the first of robot‐assisted laparoscopic surgery for a rectal cancer patient with pelvic kidney.
Three‐Dimensional Vessels‐on‐a‐Chip Based on hiPSC‐derived Vascular Endothelial and Smooth Muscle Cells

Blood vessels are composed of endothelial cells (ECs) that form the inner vessel wall and mural cells that cover the ECs to mediate their stabilization. Crosstalk between ECs and VSMCs while the ECs undergo microfluidic flow is vital for the function and integrity of blood vessels. Here, we describe a protocol to generate three‐dimensional (3D) engineered vessels‐on‐chip (VoCs) composed of vascular cells derived from human induced pluripotent stem cells (hiPSCs). We first describe protocols for robust differentiation of vascular smooth muscle cells (hiPSC‐VSMCs) from hiPSCs that are effective across multiple hiPSC lines. Second, we describe the fabrication of a simple microfluidic device consisting of a single collagen lumen that can act as a cell scaffold and support fluid flow using the viscous finger patterning (VFP) technique. After the channel is seeded sequentially with hiPSC‐derived ECs (hiPSC‐ECs) and hiPSC‐VSMCs, a stable EC barrier covered by VSMCs lines the collagen lumen. We demonstrate that this 3D VoC model can recapitulate physiological cell‐cell interaction and can be perfused under physiological shear stress using a microfluidic pump. The uniform geometry of the vessel lumens allows precise control of flow dynamics. We have thus developed a robust protocol to generate an entirely isogenic hiPSC‐derived 3D VoC model, which could be valuable for studying vessel barrier function and physiology in healthy or disease states. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
Accumulation of senescence observed in spinocerebellar ataxia type 7 mouse model

Spinocerebellar ataxia type 7 (SCA7) is a neurodegenerative disease caused by a trinucleotide CAG repeat. SCA7 predominantly causes a loss of photoreceptors in the retina and Purkinje cells of the cerebellum. Severe infantile-onset SCA7 also causes renal and cardiac irregularities. Previous reports have shown that SCA7 results in increased susceptibility to DNA damage. Since DNA damage can lead to accumulation of senescent cells, we hypothesized that SCA7 causes an accumulation of senescent cells over the course of disease. A 140-CAG repeat SCA7 mouse model was evaluated for signs of disease-specific involvement in the kidney, heart, and cerebellum, tissues that are commonly affected in the infantile form. We found evidence of significant renal abnormality that coincided with an accumulation of senescent cells in the kidneys of SCA7140Q/5Q mice, based on histology findings in addition to RT-qPCR for the cell cycle inhibitors p16Ink4a and p21Cip1 and senescence-associated ß-galactosidase (SA-ßgal) staining, respectively. The Purkinje layer in the cerebellum of SCA7140Q/5Q mice also displayed SA-ßgal+ cells. These novel findings offer evidence that senescent cells accumulate in affected tissues and may possibly contribute to SCA7’s specific phenotype.
Investigation of molecular interaction and conformational stability of disease concomitant to HLA-DRβ3

Abstract Human leucocyte antigen DRβ3 is associated with specific autoimmune thyroid disease and plays a vital role in the progression of Grave’s disease. The available crystallographic structure of the HLA DRA, DRβ3*0101, was selected and used to generate mutation at position 57 from valine amino acid to Aspartic acid (D), Glutamic acid (E), Alanine (A), and Serine (S) amino acids by computational modeling approach. Mutant models were minimized, and stable conformation was chosen based on the lowest root mean square deviation value. Molecular docking assessed the best binding affinity of ligands C1, C2, C3, and C4 with wild-type and mutant HLA-DRβ3 models. Molecular dynamics simulation studies were executed to evaluate the stability of selected hits with wild-type and mutant dock complexes. The C3 has shown good binding affinity with wild-type and selected mutants; V57A, V57E, and V57D. Structural and molecular dynamics insights reveal the differences between wild-type and mutant-type HLA-DRβ3, which could help design novel antagonist molecules against autoimmune thyroid disorder. Communicated by Ramaswamy H. Sarma
Ratio of abundances of ciliates behavioral groups as an indicator of the treated wastewater impact on rivers

A method for assessing the degree of impact of wastewater treatment plant discharge on receiving rivers was proposed, based on the structural indicators of the population of ciliated protozoa. It was shown that the ratio of attached, crawling and free-swimming forms in bottom sediments changes under the influence of discharge. In the points subject to organic pollution, the share of attached filter-feeding bacteriovorous ciliates increases in the assemblage of ciliated protozoa. The proposed Attached Form Index (AFI) takes this ratio into account. The use of AFI makes it possible to assess the restructuring of the assemblage of ciliated protozoa under the influence of point sources of pollution, to establish a zone of negative influence of runoff, to assess the degree of restoration of the aquatic ecosystem, as the influence of the pollution source weakened.
TRANSITION FROM THE INFALLING ENVELOPE TO THE KEPLERIAN DISK AROUND L1551 IRS 5

We present combined Submillimeter Array (SMA) +Atacama Submillimeter Telescope Experiment (ASTE) images of the Class I protobinary L1551 IRS 5 in the CS (J = 7–6) line, the submillimeter images of L1551 IRS 5 with the most complete spatial sampling ever achieved (0.″9–36″). The SMA image of L1551 IRS 5 in the 343 GHz dust-continuum emission is also presented, which shows an elongated feature along the northwest to southeast direction (∼160 AU × 80 AU), perpendicular to the associated radio jets. The combined SMA+ASTE images show that the high-velocity (≳1.5 km s−1) CS emission traces the structure of the dust component and shows a velocity gradient along the major axis, which is reproduced by a geometrically thin Keplerian-disk model with a central stellar mass of ∼0.5 M☉. The low-velocity (≲1.3 km s−1) CS emission shows an extended (∼1000 AU) feature that exhibits slight south (blueshifted) to north (redshifted) emission offsets, which is modeled with a rotating and infalling envelope with a conserved angular momentum. The rotational motion of the envelope connects smoothly to the inner Keplerian rotation at a radius of ∼64 AU. The infalling velocity of the envelope is ∼three times lower than the free-fall velocity toward the central stellar mass of 0.5 M☉. These results demonstrate transition from the infalling envelope to the Keplerian disk, consistent with the latest theoretical studies of disk formation. We suggest that sizable (r ∼ 50–200 AU) Keplerian disks are already formed when the protostars are still deeply embedded in the envelopes.
Individual differences in theory of mind correlate with the occurrence of false memory: A study with the DRM task

Although long-term memory and Theory of Mind (ToM) are closely related across the whole lifespan, little is known about the relationship between ToM and semantic memory. Clinical studies have documented the co-occurrence of ToM impairments and semantic memory abnormalities in individuals with autism or semantic dementia. However, to date, no study has directly investigated the existence of a relationship between ToM and semantic memory in the typical population. We addressed this gap on a sample of 103 healthy adults (M age = 22.96 years; age range = 19–35 years). Participants completed a classical false memory task tapping on semantic processes, the Deese-Roediger-McDermott (DRM) task, and two ToM tasks, the Triangles and the Reading the Mind in the Eyes task. They also completed the vocabulary scale from the Wechsler Adult Intelligence Scale. Results showed that participants’ semantic performance in the DRM task was significantly related to that in the Triangles task. Specifically, the higher participants’ ToM in the Triangles task, the higher participants’ reliance on semantic memory while making false memories in the DRM task. Our findings are consistent with the Fuzzy Trace Theory and the Weak Central Coherence account and suggest that a (partially) common cognitive process responsible for global versus detailed-focus information processing could underlie these two abilities.
Conservation of transcriptional regulation by BRCA1 and BARD1 in Caenorhabditis elegans

Abstract The tumor-suppressor proteins BRCA1 and BARD1 function as an E3 ubiquitin ligase to facilitate transcriptional repression and DNA damage repair. This is mediated in-part through its ability to mono-ubiquitylate histone H2A in nucleosomes. Studies in Caenorhabditis elegans have been used to elucidate numerous functions of BRCA1 and BARD1; however, it has not been established that the C. elegans orthologs, BRC-1 and BRD-1, retain all the functions of their human counterparts. Here we explore the conservation of enzymatic activity toward nucleosomes which leads to repression of estrogen-metabolizing cytochrome P450 (cyp) genes in humans. Biochemical assays establish that BRC-1 and BRD-1 contribute to ubiquitylation of histone H2A in the nucleosome. Mutational analysis shows that while BRC-1 likely binds the nucleosome using a conserved interface, BRD-1 and BARD1 have evolved different modes of binding, resulting in a difference in the placement of ubiquitin on H2A. Gene expression analysis reveals that in spite of this difference, BRC-1 and BRD-1 also contribute to cyp gene repression in C. elegans. Establishing conservation of these functions in C. elegans allows for use of this powerful model organism to address remaining questions regarding regulation of gene expression by BRCA1 and BARD1.
An Accessible Communication System for Population-Based Genetic Testing: Development and Usability Study

Background Genetic testing uptake is low, despite the well-established connection between pathogenic variants in certain cancer-linked susceptibility genes and ovarian cancer risk. Given that most major insurers cover genetic testing for those with a family history suggestive of hereditary cancer, the issue may lie in access to genetic testing. Remotely accessible web-based communication systems may improve awareness, and uptake, of genetic testing services. Objective This study aims to present the development and formative evaluation of the multistep web-based communication system required to support the implementation of, and access to, genetic testing. Methods While designing the multistep web-based communication system, we considered various barriers and facilitators to genetic testing, guided by dimensions of accessibility. In addition to conducting usability testing, we performed ongoing assessments focusing on the function of the web-based system and participant response rates, with the goal of continuing to make modifications to the web-based communication system as it is in use. Results The combined approach of usability testing and expert user experience consultation resulted in several modifications to the multistep web-based communication system, including changes that related to imagery and content, web accessibility, and general organization of the web-based system. All recommendations were made with the goal of improving the overall accessibility of the web-based communication system. Conclusions A multistep web-based communication system appears to be an effective way to address many potential barriers to access, which may otherwise make genetic testing difficult for at-risk individuals to participate in. Importantly, some dimensions of access were easy to assess before study recruitment, but other aspects of the communication system required ongoing assessment during the implementation process of the Making Genetic Testing Accessible study.
AN UNBIASED 1.3 mm EMISSION LINE SURVEY OF THE PROTOPLANETARY DISK ORBITING LkCa 15

The outer (>30 AU) regions of the dusty circumstellar disk orbiting the ∼2–5 Myr old, actively accreting solar analog LkCa 15 are known to be chemically rich, and the inner disk may host a young protoplanet within its central cavity. To obtain a complete census of the brightest molecular line emission emanating from the LkCa 15 disk over the 210–270 GHz (1.4–1.1 mm) range, we have conducted an unbiased radio spectroscopic survey with the Institute de Radioastronomie Millimétrique (IRAM) 30 m telescope. The survey demonstrates that in this spectral region, the most readily detectable lines are those of CO and its isotopologues 13CO and C18O, as well as HCO+, HCN, CN, C2H, CS, and H2CO. All of these species had been previously detected in the LkCa 15 disk; however, the present survey includes the first complete coverage of the CN (2–1) and C2H (3–2) hyperfine complexes. Modeling of these emission complexes indicates that the CN and C2H either reside in the coldest regions of the disk or are subthermally excited, and that their abundances are enhanced relative to molecular clouds and young stellar object environments. These results highlight the value of unbiased single-dish line surveys in guiding future high-resolution interferometric imaging of disks.
VARIABLE GAMMA-RAY EMISSION INDUCED BY ULTRA-HIGH ENERGY NEUTRAL BEAMS: APPLICATION TO 4C +21.35

The flat-spectrum radio quasar 4C +21.35 (PKS 1222+216) displays prominent nuclear infrared emission from ≈1200 K dust. A 70–400 GeV flare with ≈10 minute variations during half an hour of observations was found by the MAGIC telescopes, and GeV variability was observed on sub-day timescales with the Large Area Telescope on Fermi. We examine 4C +21.35, assuming that it is a source of ultra-high energy cosmic rays (UHECRs). UHECR proton acceleration in the inner jet powers a neutral beam of neutrinos, neutrons, and γ-rays from pγ photopion production. The radiative efficiency and production spectra of neutrals formed through photohadronic processes with isotropic external target photons of the broad-line region (BLR) and torus are calculated. Secondary radiations made by this process have a beaming factor ∝δ5D, where δD is the Doppler factor. The pair-production optical depth for γ-rays and the photopion efficiency for UHECR neutrons as they pass through external isotropic radiation fields are calculated. If target photons come from the BLR and dust torus, large Doppler factors, δD ≳ 100, are required to produce rapidly variable secondary radiation with isotropic luminosity ≳ 1047 erg s−1 at the pc scale. The γ-ray spectra from leptonic secondaries are calculated from cascades initiated by the UHECR neutron beam at the pc-scale region and fit to the flaring spectrum of 4C +21.35. Detection of ≳ 100 TeV neutrinos from 4C +21.35 or other very high energy γ-ray blazars with IceCube or KM3NeT would confirm this scenario.
Understanding decision-making and educational need in the management of pediatric patients with moderate-to-severe asthma: analysis of a US-based clinician survey

Abstract Background: Asthma creates a substantial burden on the pediatric healthcare system, particularly by those with moderate-to-severe disease. As few studies have been published specific to clinician evaluation and management of this population, this analysis serves to begin to identify educational needs and potential clinical impact.Methods: We conducted a case-based survey of US general pediatric (n = 227), allergy (n = 158), and pediatric pulmonology (n = 70) clinicians to understand the current approach to management of pediatric patients with moderate-to-severe asthma.Results: Results show inconsistencies in guideline adherence, identification of disease severity and control, referral practices, and knowledge of phenotypes, which may impact clinical decision making. While most clinicians refer to guidelines when managing pediatric patients with moderate-to-severe asthma, there is no preferred set, and they find the guidelines to be moderately useful. General pediatricians are more likely than specialists to have difficulty distinguishing control and severity and may not be familiar with the concept of asthma phenotype. Most surveyed pediatricians indicated they refer patients to a specialist, but did not always do so in the cases. Clinicians have little consensus on the best approach to manage patients with uncontrolled symptoms after optimized therapy. Many clinicians may have uncertainty about how elevated eosinophils or FeNO could affect patient management. Further, there may be gaps in incorporating parents and pediatric patients into shared decision-making in their care.Conclusions: Understanding the needs of those managing patients with moderate-to-severe asthma is critical to developing effective continuing education activities to improve patient outcomes.
The risk of aspirin induced postoperative hemorrhage: a systematic review and meta-analysis

ABSTRACT Background and Objective Current guidelines recommend aspirin maintenance for high-risk endoscopic procedures. Some Asian physicians noticed increasing postoperative bleeding in patients taking aspirin. We aimed to explore whether risk of postoperative hemorrhage due to aspirin differs in the East and the West. Methods PubMed, EMBASE and Cochrane library database were systematically reviewed. We only included trials that met our criteria. Results There is significant association between aspirin and postoperative bleeding (P < 0.001), especially in Eastern population (data from Japan, Korea, Turkey and China, P < 0.001). Result from the West (data from America, Canada and Australia) had no statistical significance (P = 0.07). For Easterners, aspirin increased bleeding risk after endoscopic submucosal dissection (ESD) and endoscopic sphincterotomy (EST). For Westerners, aspirin increased bleeding risk post endoscopic mucosal resection (EMR). For patients undergoing ESD, those who continued to receive aspirin had higher bleeding risk than patients who interrupted it for more than 7 days (P = 0.005). Conclusion Aspirin increases risk of postoperative hemorrhage. Easterners are more likely to suffer from bleeding after aspirin administration than Westerners. Stopping aspirin for more than 7 days may be advisable to control bleeding post ESD for patients with low risk of thrombosis.
A survival analysis of bipolar steroid‐eluting and unipolar nonsteroid‐eluting epicardial leads

Epicardial pacemakers are known as an alternative for endocardial pacemakers in some cases such as heart block, and complex congenital heart diseases. Considering recent advances and improvement of epicardial lead subtypes, it is essential to investigate the long‐term function of them. In this study, we aimed to assess the sensing and pacing characteristics, and survival of bipolar steroid‐eluting and unipolar nonsteroid‐eluting epicardial pacemakers.
Updated management for antibody-mediated rejection: opportunity to prolong kidney allograft survival

Purpose of review Antibody-mediated rejection (ABMR) is an important barrier to achieve long-term kidney allograft survival. Human leukocyte antibody (HLA)-incompatibility and ABO-incompatibility are the two main mechanisms of ABMR. Nevertheless, the advances in managing ABMR have changed the paradigm for kidney transplantation. This review aimed to emphasize the HLA-incompatibility and ABO-incompatibility kidney transplant and update the management of ABMR. Recent findings HLA-incompatibility kidney transplantation is a strong risk factor for ABMR. Donor-specific antibody (DSA) is a surrogate biomarker that prevents long-term allograft survival. The standard treatment for ABMR has unfavorable results. New drugs that target the B cell are a promising approach to treat ABMR. In the past, ABO-incompatibility kidney donor was an absolute contraindication but now, it is widely accepted as an alternative organ resource. The advancement of ABO antibody removal and B-cell depletion therapy has been successfully developed. ABO isoagglutination remains the main biomarker for monitoring ABMR during the transplantation process. C4d staining without inflammation of the kidney allograft is the marker for the accommodation process. Summary With the shortage of organ donors, transplant experts have expanded the organ resources and learned how to overcome the immunological barriers by using novel biomarkers and developing new treatments that support long-term graft survival.