Miracle-dz/UniFAR-FTUs huggingface dataset:
Accumulation of membrane glycoproteins in lysosomes requires a tyrosine residue at a particular position in the cytoplasmic tail

Human lysosome membrane glycoprotein h-lamp-1 is a highly N- glycosylated protein found predominantly in lysosomes, with low levels present at the cell surface. The signal required for delivery of h-lamp- 1 to lysosomes was investigated by analyzing the intracellular distribution of h-lamp-1 with altered amino acid sequences expressed from mutated cDNA clones. A cytoplasmic tail tyrosine residue found conserved in chicken, rodent, and human deduced amino acid sequences was discovered to be necessary for efficient lysosomal transport of h- lamp-1 in COS-1 cells. In addition, the position of the tyrosine residue relative to the membrane and carboxyl terminus also determined lysosomal expression. Supplanting the wild-type h-lamp-1 cytoplasmic tail onto a cell surface reporter glycoprotein was sufficient to cause redistribution of the chimera to lysosomes. A similar chimeric protein replacing the cytoplasmic tyrosine residue with an alanine was not expressed in lysosomes. Altered proteins that were not transported to lysosomes were found to accumulate at the cell surface, and unlike wild- type lysosomal membrane glycoproteins, were unable to undergo endocytosis. These data indicate that lysosomal membrane glycoproteins are sorted to lysosomes by a cytoplasmic signal containing tyrosine in a specific position, and the sorting signal may be recognized both in the trans-Golgi network and at the cell surface.
Building Efficiency and Sustainability in the Tropics ( SinBerBEST ) Title Model Predictive Control of Regulation Services from Commercial Buildings to the Smart Grid

We first demonstrate that the demand-side flexibility of the Heating Ventilation and Air Conditioning (HVAC) system of a typical commercial building can be exploited for providing frequency regulation service to the power grid using at-scale experiments. We then show how this flexibility in power consumption of building HVAC system can be leveraged for providing regulation service. To this end, we consider a simplified model of the power grid with uncertain demand and generation. We present a Model Predictive Control (MPC) scheme to direct the ancillary service power flow from buildings to improve upon the classical Automatic Generation Control (AGC) practice. We show how constraints such as slow and fast ramping rates for various ancillary service providers, and short-term load forecast information can be integrated into the proposed MPC framework. Finally, we provide extensive simulation results to illustrate the effectiveness of the proposed methodology for enhancing grid frequency regulation.
Peritoneal membrane phosphate transport status: a cornerstone in phosphate handling in peritoneal dialysis.

BACKGROUND AND OBJECTIVES Phosphate control impacts dialysis outcomes. Our aim was to define peritoneal phosphate transport in peritoneal dialysis (PD) and to explore its association with hyperphosphatemia, phosphate clearance (PPhCl), and PD modality. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS Two hundred sixty-four patients (61% on continuous ambulatory PD [CAPD]) were evaluated at month 12. PPhCl was calculated from 24-hour peritoneal effluent. Phosphate (Ph) and creatinine (Cr) dialysate/plasma (D/P) were calculated at a 4-hour 3.86% peritoneal equilibration test. RESULTS D/PPh correlated with D/PCr. PPhCl correlated better with D/PPh than with D/PCr. Prevalence of hyperphosphatemia (>5.5 mg/dl) was 30%. In a multiple regression analysis, only residual renal function was independently, negatively associated with hyperphosphatemia; in anuric patients, only D/PPh was an independent factor predicting hyperphosphatemia. D/PPh was 0.57 ± 0.10, and according to this, 16% of the patients were fast, 31% were fast-average, 35% were slow-average, and 17% were slow transporters. PPhCl was 37.5 ± 11.7 L/wk; it was lower in the slow transporter group (31 ± 14 L/wk). Among fast and fast-average transporters, PPhCl was comparable in both PD modalities. In comparison to automated PD, CAPD was associated with increased PPhCl among slow-average (36 ± 8 versus 32 ± 7 L/wk) and slow transporters (34 ± 15 versus 24 ± 9 L/wk). CONCLUSIONS In hyperphosphatemic, particularly anuric, patients, optimal PD modality should consider peritoneal phosphate transport characteristics. Increasing dwell times and transfer to CAPD are effective strategies to improve phosphate handling in patients with inadequate phosphate control on automated PD.
Microbial Populations Stimulated for Hexavalent Uranium Reduction in Uranium Mine Sediment

ABSTRACT Uranium-contaminated sediment and water collected from an inactive uranium mine were incubated anaerobically with organic substrates. Stimulated microbial populations removed U almost entirely from solution within 1 month. X-ray absorption near-edge structure analysis showed that U(VI) was reduced to U(IV) during the incubation. Observations by transmission electron microscopy, selected area diffraction pattern analysis, and energy-dispersive X-ray spectroscopic analysis showed two distinct types of prokaryotic cells that precipitated only a U(IV) mineral uraninite (UO2) or both uraninite and metal sulfides. Prokaryotic cells associated with uraninite and metal sulfides were inferred to be sulfate-reducing bacteria. Phylogenetic analysis of 16S ribosomal DNA obtained from the original and incubated sediments revealed that microbial populations were changed from microaerophilic Proteobacteria to anaerobic low-G+C gram-positive sporeforming bacteria by the incubation. Forty-two out of 94 clones from the incubated sediment were related to sulfate-reducing Desulfosporosinus spp. , and 23 were related to fermentative Clostridium spp. The results suggest that, if in situ bioremediation were attempted in the uranium mine ponds, Desulfosporosinus spp. would be a major contributor to U(VI) and sulfate reduction and Clostridium spp. to U(VI) reduction.
Comparison between a hybrid digital and analog beamforming system and a fully digital Massive MIMO system with adaptive beamsteering receivers in millimeter-Wave transmissions

Massive MIMO systems are known to be a very promising solution for future 5G systems. The attractiveness of using such a system for millimeter Wave (mmWave) transmissions comes partly from the reduced size of a many antenna base station. Furthermore, the beamforming gains that they allow is highly suited to combat the high path losses experienced at such wavelengths. Unfortunately the complexity to implement a mmWave Massive MIMO system comes with the difficulty to have a high number of stacked Radio Frequency (RF) chains. Hybrid Analog and Digital Beamforming systems have then emerged to retain a high number of antennas without as many RF chains in order to keep high beamforming gains. In this paper we first describe a User Equipment (UE) solution allowing the system to form a beam that adapts to its own movement so that it always focuses its energy toward the base station, using an on board analog array and an Inertial Measurement Unit. Then we compare the performance of the known Hybrid solution with a fully digital Massive MIMO system, having as many RF chains as the Hybrid system, but serving UEs with beamforming abilities. Mostly we emphasize how such a system allows for great flexibility and evolution, both traits being invaluable features in many future networks.
Metabolic Response of Blood Cells to Synthetic Graft-Materials with Special Reference to a Fluoromer Passivated Dacron® Graft. An in Vitro Study Using Microcalorimetry

Microcalorimetry was used to study in vitro the metabolic response from human platelets and leukocytes when incubated with three different synthetic graft-materials. The graft to be studied primarily was Fluoromer Passivated Dacron (FPD) which was compared with ePTFE and with a knitted Teflon graft. A rapid increase in the metabolic activity of platelets was observed, followed by a steady-state for more than one hour, while the platelet metabolism did not differ among the various graft-materials. Leukocytes incubated with FPD showed a high initial metabolism, with a peak after about 15 minutes. After 60 minutes the metabolic response had reached control values. ePTFE and Teflon grafts differed significantly from FPD, without causing any peak metabolic activity. It may be concluded that FPD and ePTFE grafts, as evaluated in vitro, activate platelets to the same extent, while FPD causes a more extensive leukocyte activation. Whether these findings can be interpreted as differences in thrombogenicity and inflammatory responses has not been proven, but seems probable. This in vitro method should make it possible to further study human responses to synthetic materials a method possibly more reliable than animal experiments.
Mutations in the Drosophila hook gene inhibit endocytosis of the boss transmembrane ligand into multivesicular bodies

Transmembrane ligands can be internalized across cell boundaries into receptor-expressing cells. In the developing Drosophila eye imaginal disc, the bride of sevenless transmembrane protein (boss) is expressed on the surface of R8 cells. After internalization into neighboring R7 cells, the boss protein accumulates in multivesicular bodies. In a search for genes that affect this cell-type-specific pattern of boss endocytosis, we found that mutations in the hook gene inhibit the accumulation of boss in multivesicular bodies of R7 cells. In addition, hook flies exhibit pleiotropic phenotypes including abnormal bristle morphology and eye degeneration. The wild-type-pattern of boss endocytosis was restored in hook mutants by a genomic rescue fragment containing the hook gene or by a hook cDNA expressed in R7 cells under control of a sevenless (sev) enhancer. The hook gene encodes a novel cytoplasmic protein of 679 amino acids with a central coiled-coil domain of some 200 amino acids. Truncated, epitope-tagged hook proteins coimmunoprecipitated the full-length protein, indicating dimerization mediated by the coiled-coil domain. The hook protein localizes to vesicular structures that are part of the endocytic compartment. The requirement of the hook protein in R7 cells for the accumulation of boss protein in multivesicular bodies, and the localization of the hook protein to endocytic vesicles indicate that the hook gene encodes a novel component of the endocytic compartment that plays an important role in the endocytosis of transmembrane ligands or their transport to multivesicular bodies.
Words in the Mind : Exploring the relationship between word association and lexical development

Vocabulary acquisition in both L1 and L2 has long relied on the principle of associative language learning to help understand and explain the process of lexical acquisition in the mind of the language learner. To date, L2 studies in this area have however delivered somewhat contentious findings. This study seeks to revisit some of the relevant issues and gain some insight into how words are held in the mind – or, more precisely, to understand the relationship between lexical development and the process of word association (WA). To better understand the relationship between lexical semantics and the mental lexicon, this investigation explores and compares the word associations of a group of Japanese English speakers and a group of English native speakers. It attempts to understand how mental links are made between lexical items in the mental lexicon, a process commonly believed to partially support vocabulary acquisition. Initial findings support general trends in this research area, namely that the word-associations of native and foreign speakers differ in some respects. These however, do not occur to the extent that they may constitute a pattern that may distinguish native from foreign speakers. Findings further highlight the importance of lexical semantics and phonological aspects during word learning, while the importance of developing a robust methodology for the analysis of word association are briefly addressed. To conclude, implications for WA research, the mental lexicon and L2 language learning and teaching are discussed.
Objective Measures in Cochlear Implants

Bargaining with reading habit is no need. Reading is not kind of something sold that you can take or not. It is a thing that will change your life to life better. It is the thing that will give you many things around the world and this universe, in the real world and here after. As what will be given by this objective measures in cochlear implants, how can you bargain with the thing that has many benefits for you?
Coincident pre‐ and postsynaptic activity downregulates NKCC1 to hyperpolarize ECl during development

In the mature CNS, coincident pre‐ and postsynaptic activity decreases the strength of γ‐aminobutyric acid (GABA)A‐mediated inhibition through a Ca2+‐dependent decrease in the activity of the neuron‐specific K+‐Cl– cotransporter KCC2. In the present study we examined whether coincident pre‐ and postsynaptic activity can also modulate immature GABAergic synapses, where the Na+‐K+‐2Cl– (NKCC1) cotransporter maintains a relatively high level of intracellular chloride ([Cl–]i). Dual perforated patch‐clamp recordings were made from cultured hippocampal neurons prepared from embryonic Sprague–Dawley rats. These recordings were used to identify GABAergic synapses where the reversal potential for Cl– (ECl) was hyperpolarized with respect to the action potential threshold but depolarized with respect to the resting membrane potential. At these synapses, repetitive postsynaptic spiking within ± 5 ms of GABAergic synaptic transmission resulted in a hyperpolarizing shift of ECl by 10.03 ± 1.64 mV, increasing the strength of synaptic inhibition. Blocking the inward transport of Cl– by NKCC1 with bumetanide (10 µm) hyperpolarized ECl by 16.14 ± 4.8 mV, and prevented this coincident activity‐induced shift of ECl. The bumetanide‐induced hyperpolarization of ECl occluded furosemide, a K+‐Cl– cotransporter antagonist, from producing further shifts in ECl. Together, this indicates that brief coincident pre‐ and postsynaptic activity strengthens inhibition through a regulation of NKCC1. This study further demonstrates ionic plasticity as a mechanism underlying inhibitory synaptic plasticity.
BIRON, where are you? Enabling a robot to learn new places in a real home environment by integrating spoken dialog and visual localization

An ambitious goal in modern robotic science is to build mobile robots that are able to interact as companions in real world environments. Especially for caretaking of elderly people a system robustly working at private homes is essential, requiring a very natural and human oriented way of communication. Since home environments are usually very individual a first task for a newly acquired robot is to get familiar with its new environment. This paper gives a short overview on how we integrated a vision based localization using the advantages of a very modular architecture and extending a spoken dialog system for online labeling and interaction about different locations. We present results from the integrated system working in a real, fully furnished home environment where it was able to learn the names of different rooms. This system enables us to perform real user studies in future without the need to fall back to Wizard-of-Oz experiments. Ongoing work aims at enabling the robot to take initiative by asking for unknown locations. A future extension is the ability to generalize over features of known rooms to make predictions when encountering unknown rooms
Dynamical cognitive network-an extension of fuzzy cognitive map

We present a dynamic cognitive network (DCN) which is a systematic extension of the fuzzy cognitive map (FCM). Each concept in a dynamic cognitive network can have its own value set, depending on how precisely it needs to be described in the network. This enables the dynamic cognitive network to describe the strength of the cause and the degree of the effect. This ability also enables DCN to carry out inferences via numerical calculation instead of symbolic deduction. The arcs of DCN define dynamic relationships between concepts and describe the causal procedures. DCN overcomes the problems of FCM without losing the essential advantages.
Effect of rhEPO administration on serum levels of sTfR and cycling performance.

PURPOSE We assessed the possibility of using soluble transferrin receptor (sTfR) as an indicator of doping with recombinant erythropoietin (rhEPO). METHODS A double-blind, placebo-controlled study was conducted with the administration of 5,000 U of rhEPO (N = 10) or placebo (N = 10) three times weekly (181-232 U x kg(-1) x wk-1) for 4 wk to male athletes. We measured hematocrit and the concentration of hemoglobin, sTfR, ferritin, EPO, and quantified the effects on performance by measuring time to exhaustion and maximal oxygen uptake (VO2max) on a cycle ergometer. RESULTS Hematocrit increased from 42.7 +/- 1.6% to 50.8 +/- 2.0% in the EPO group, and peaked 1 d after treatment was stopped. In the EPO group, there was an increase in sTfR (from 3.1 +/- 0.9 to 6.3 +/- 2.3 mg x L(-1) , P < 0.001) and in the ratio between sTfR and ferritin (sTfR-ferritin(-1)) (from 3.2 +/- 1.6 to 11.8 +/- 5.1, P < 0.001). The sTfR increase was significant after 1 wk of treatment and remained so for 1 wk posttreatment. Individual values for sTfR throughout the study period showed that 8 of 10 subjects receiving rhEPO, but none receiving placebo, had sTfR levels that exceeded the 95% confidence interval for all subjects at baseline (= 4.6 mg x L(-1)). VO2max increased from 63.6 +/- 4.5 mL x kg(-1) x min(-1) before to 68.1 +/- 5.4 mL x kg(-1) x min(-1) 2 d post rhEPO administration (7% increase, P = 0.001) in the EPO group. Hematocrit, sTfR, sTfR-ferritin(-1), and VO2max did not change in the placebo group. CONCLUSION Serum levels of sTfR may be used as an indirect marker of supranormal erythropoiesis up to 1 wk after the administration of rhEPO, but the effects on endurance performance outlast the increase in sTfR.
Innate preference and learning of colour in the male cotton bollworm moth, Helicoverpa armigera

ABSTRACT We investigated colour discrimination and learning in adult males of the nocturnal cotton bollworm moth, Helicoverpa armigera, under a dim light condition. The naive moths preferred blue and discriminated the innately preferred blue from several shades of grey, indicating that the moths have colour vision. After being trained for 2 days to take nectar at a yellow disc, an innately non-preferred colour, moths learned to select yellow over blue. The choice distribution between yellow and blue changed significantly from that of naive moths. However, the dual-choice distribution of the trained moths was not significantly biased to yellow: the preference for blue is robust. We also tried to train moths to grey, which was not successful. The limited ability to learn colours suggests that H. armigera may not strongly rely on colours when searching for flowers in the field, although they have the basic property of colour vision. Summary: Helicoverpa armigera, the nocturnal cotton bollworm moth, learns colours upon feeding, and discriminates colour discs depending on the chromatic contents irrespective of brightness.
Kinin Peptides Enhance Inflammatory and Oxidative Responses Promoting Apoptosis in a Parkinson's Disease Cellular Model

Kinin peptides ubiquitously occur in nervous tissue and participate in inflammatory processes associated with distinct neurological disorders. These substances have also been demonstrated to promote the oxidative stress. On the other hand, the importance of oxidative stress and inflammation has been emphasized in disorders that involve the neurodegenerative processes such as Parkinson's disease (PD). A growing number of reports have demonstrated the increased expression of kinin receptors in neurodegenerative diseases. In this study, the effect of bradykinin and des-Arg10-kallidin, two representative kinin peptides, was analyzed with respect to inflammatory response and induction of oxidative stress in a PD cellular model, obtained after stimulation of differentiated SK-N-SH cells with a neurotoxin, 1-methyl-4-phenylpyridinium. Kinin peptides caused an increased cytokine release and enhanced production of reactive oxygen species and NO by cells. These changes were accompanied by a loss of cell viability and a greater activation of caspases involved in apoptosis progression. Moreover, the neurotoxin and kinin peptides altered the dopamine receptor 2 expression. Kinin receptor expression was also changed by the neurotoxin. These results suggest a mediatory role of kinin peptides in the development of neurodegeneration and may offer new possibilities for its regulation by using specific antagonists of kinin receptors.
The development in body mass index, overweight and obesity in three regions in Denmark.

BACKGROUND The prevalence of overweight and obesity has increased markedly the past decades. However, recent studies have indicated that the development differ between different socio-economic groups and different geographic regions. The aim of this study was to assess the development in prevalence of overweight and obesity from 2006/2007 to 2010 by age, gender, socio-economic factors and geographical regions. METHODS Two cross-sectional surveys in three regions in Denmark (The Capital Region of Denmark, The Central Denmark Region and The North Denmark Region) were performed in 2006/2007 and 2010. A random sample of citizens aged more than or equal to 25 years was invited to participate. The overall response rate was 57.5% (n = 177 076). Data from questionnaire and central registers were included. RESULTS In 2006/2007, the prevalence of overweight, including obesity, was 54.3% and 36.8% among men and women, respectively. Of the overweight men 12.8% were obese and 11.8% women were obese. The prevalence was highest in the Northern region and among those who were older, had short education, was outside labour market, had low income and residents in rural areas. In 2010, the prevalence of overweight had increased to 56.3% and 39.6% in men and women, respectively (P < 0.0001). However, overweight increased the most in the Northern Region whereas no significant increase in body mass index was found among men in the Capital Region. Among women prevalence of overweight increased but not in those retired or above 60 years, and with high income. CONCLUSION The prevalence of overweight and obesity was high and increased. The development, however, was heterogenic.
Assistance for Telepresence by Stereovision-Based Augmented Reality and Interactivity in 3D Space

In this paper, we describe some use of mixed reality as a new assistance for performing teleoperation tasks in remote scenes. We will start by a brief classification of augmented reality. This paper then describes the principle of our mixed reality system in teleoperation. It tackles the problem of scene registration using a manmachine cooperative and multisensory vision system. The system provides the operator with powerful sensorial feedback as well as appropriate tools to build (and update automatically) the geometric model of the perceived scene. We describe a new interactive approach combining image analysis and mixed reality techniques for assisted 3D geometric and semantic modeling. At the end of this paper, we describe applications in nuclear plants with results in 3D positioning.