Miracle-dz/UniFAR-FTUs huggingface dataset:
Prevalence and Factors Associated with the Anxio-Depressive Symptoms of the Families of Patients in Intensive Care Unit at Gabriel Toure University Hospital, Bamako, Mali

Introduction: Hospitalization in intensive care is a source of stress and anxiety for close to the patients. Anxio-depressive symptoms appear to be common in intensive care and their prevalence is poorly evaluated. The objective of this study was to evaluate the prevalence of anxio-depressive symptoms in families. Materials and method: Observational prospective study in families of patients hospitalized in the intensive care unit for a period of 5 months. Symptoms of anxiety and depression were measured using Hospital Anxiety and Depression Scale (HADS) during hospitalization. Anxiety and depression were defined by a score greater than 10. Factors associated with the onset of anxious-depressive symptoms were sought. The prevalence of post-traumatic stress disorder in close relatives was measured by the Impact Event Scale-Revised scale (IES-R). Results: A total of 107 patients were admitted to the intensive care unit, of which 49 families agreed to participate in our study. Overall mortality was 32.2% during this period. Fifty-eight (58) patients were not included for the following reasons: death or hospitalization of less than 48 hours, refusal of families, institution, and lack of parents speaking French. Forty-nine (49) relatives completed the HADS questionnaire. Forty-three families completed the IES-R questionnaire, a return rate of 87.7%. The prevalence of anxiety was 61.2% among parents in early hospitalization. The level of anxiety was significantly associated with male parents (p = 0.035) and those with lower education (p = 0.046). The prevalence of depressive symptoms in parents was evaluated 53% at D3. Education level (p = 0.048) and male parents (p = 0.048) appeared to be a significant depression factor. The prevalence of post-traumatic stress disorder was 55.1% among the relatives of the patients. The lack of co-morbidity in admission patients was significantly associated with the occurrence of post-traumatic stress disorder in the family. Conclusion: The prevalence of anxiety and depression symptoms was high in our study. Men were at greater risk of developing these symptoms. The parents of the patients carburized appear as a population with higher risk manifestation of psychological disorders; further research is needed in this group.
Biotechnology and Bioengineering

Laboratory for Integrated Micro Mechatronic Systems, CNRS UMI 2820, Institute of Industrial Science, The University of Tokyo, Tokyo, Japan Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan Division of Genomic Medicine, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan Laboratory of Molecular Pharmacokinetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
Biotechnology and Bioengineering

Funding information Program to accomplish research activities of female researchers; Scholar project program from Toyota Physical Chemical Research Institute; Project Focused on Developing Key Evaluation Technology: Development of Platform Technology for Drug Discovery through Application of Regenerative Medicine commissioned by the Japanese Agency for Medical Research and Development (AMED), Grant/Award Number: JP19be0604001 Abstract The creation of a blueprint for stem cell bioprocess development that it is easily readable and shareable among those involved in the construction of the bioprocess is a necessary step toward full‐fledged bioprocess integration. The blueprint provides the culturing tools and methodologies, designed to highlight knowledge gaps within biological sciences and bioengineering. This review highlights a blueprint for stem cell bioprocessing development using a landscape architecture approach that can aid the development of culture technologies and tools that satisfy the demands for stem cell‐derived products for use in clinical and industrial applications. This work is intended to provide insights to cell biologists, geneticists, bioengineers, and clinicians seeking knowledge outside of their field of expertise and fosters a leap from a reductionist approach to one, that is, globally integrated in stem cell bioprocessing.
Biotechnology and Bioengineering

Correspondence Misha Ali and Qayyum Husain, Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India. Email: misha11biochem@gmail.com (MA) and qayyumbiochem@gmail.com (QH) Abstract Enzyme engineering is a powerful tool to fine‐tune the enzymes. It is a technique by which the stability, activity, and specificity of the enzymes can be altered. The characteristic properties of an enzyme can be amended by immobilization and protein engineering. Among them, protein engineering is the most promising, as in addition to amending the stability and activity, it is the only way to modulate the specificity and stereoselectivity of enzymes. The current review sheds light on protein engineering and the approaches applied for it on the basis of the degree of knowledge of structure and function of enzymes. Enzymes, which have been engineered are also discussed in detail and categorized on the basis of their respective applications. This will give a better insight into the revolutionary changes brought by protein engineering of enzymes in various industrial and environmental processes.
Biotechnology and Bioengineering

Funding information National Institute of Arthritis and Musculoskeletal and Skin Diseases, Grant/ Award Number: K08AR068469; National Institutes of Health Director's Program, Grant/Award Number: DP2 GM229133; National Cancer Institute, Grant/Award Number: U54 CA210184; National Institutes of Health, Grant/Award Number: T35 OD010941; National Institutes of Health National Research Service Award, Grant/Award Number: 2T32GM008267; National Science Foundation Graduate Research Fellowship, Grant/Award Number: DGE‐1650441 Abstract Widespread therapeutic and commercial interest in recombinant mucin technology has emerged due to the unique ability of mucin glycoproteins to hydrate, protect, and lubricate biological surfaces. However, recombinant production of the large, highly repetitive domains that are characteristic of mucins remains a challenge in biomanufacturing likely due, at least in part, to the inherent instability of DNA repeats in the cellular genome. To overcome this challenge, we exploit codon redundancy to encode desired mucin polypeptides with minimal nucleotide repetition. The codon‐scrambling strategy was applied to generate synonymous genes, or “synDNAs,” for two mucins of commercial interest: lubricin and mucin 1. Stable, long‐term recombinant production in suspension‐ adapted human 293‐F cells was demonstrated for the synonymous lubricin complementary DNA (cDNA), which we refer to as SynLubricin. Under optimal conditions, a 293‐F subpopulation produced recombinant SynLubricin at more than 200 mg/L of media and was stable throughout 2 months of continuous culture. Functionality tests confirmed that the recombinant lubricin could effectively inhibit cell adhesion and lubricate cartilage explants. Together, our work provides a viable workflow for cDNA design and stable mucin production in mammalian host production systems.
ENVISAT ASAR data for agriculture mapping in Zhangzhou, Fujian Province, China

This paper investigates the potential of ENVISAT ASAR data for agriculture crop mapping. The multi-temporal ASAR data with different polarization are used. The study area is located in Zhangzhou, Fujian province, China. Based on the analysis of radar signature of different land cover, crop types like rice, water bamboo and banana are classified using object oriented classifier. Compared to the field survey data, the accuracy of rice, banana and water bamboo in study area is more than 80%. The object oriented classifier is suitable for SAR data classification.
International Journal of Public Health Science (IJPHS)

Received Aug 18, 2021 Revised Nov 7, 2021 Accepted Nov 19, 2021 This study reported the prevalence of smartphone addiction among Gen X and Gen Y in Sarawak, Malaysia. This study compared the two generations, X and Y, on smartphone addictive behaviour. It is important to understand their addiction to smartphones. Two hundred thirty-six participants were recruited using a Facebook advertisement, consisting of 122 males and 114 females aged 16 to 55. The smartphone addiction scale-malay (SAS-M) was used to assess smartphone addiction. The data was analysed using IBM SPSS. The result shows that Gen Y is more addicted to a smartphone than Gen X for all components of SAS-M, but there is no significant difference between female and male users for both generations in smartphone usage. In addition, there is a significant difference in the cyberspace-oriented relationship between Gen X and Gen Y. Furthermore, Gen Y had lost control of the use of smartphones compared to Gen X, which reflects the overuse and primacy components that lead to daily life disturbances. Positive anticipation and withdrawal were also observed between the two generations. In conclusion, there is a significant difference between Gen X and Gen Y in smartphone addictive behaviour, implying that Gen Y is a more compulsive user than Gen X. It is important to understand this addictive behaviour and provide an intervention measure to ensure that this will not become a psychological issue. The intervention measure is important to improve mental health and psychological wellbeing.
International Journal of Public Health Science (IJPHS)

Received Feb 13, 2023 Revised Aug 7, 2023 Accepted Aug 30, 2023 This study aimed to investigate the characteristics of COVID-19 patients in Thailand and develop a data model for analyzing these characteristics. A total of 1,888,941 cases from the Thailand Department of Disease Control website from January 12, 2020, to October 29, 2021, were analyzed, and 20,110 cases were selected for further analysis. The two-step cluster analysis method was used to cluster the data according to four variables: nationality, occupation, patient type, and risk groups. The results showed the presence of three groups of COVID-19 patients. Group 1 consisted of 5,883 workers in trade and service occupations who had contact with the public and were either Thai nationals or from abroad. Group 2 was the largest cluster, consisting of 7,420 migrant workers classified as foreigners and working in industrial settings. Group 3 consisted of 6,870 cases of indirect transmission, with individuals in this group infected through close contact with family members or individuals in the first two groups. This clustering approach offers valuable insights for pandemic management, aiding in identifying high-risk groups and developing tailored interventions. In future outbreaks with similar characteristics, such as airborne transmission, contact infection, or super spreader events, our model can serve as a valuable tool for devising effective management plans and countermeasures. In conclusion, this study emphasizes the significance of cluster analysis in understanding the dynamics of COVID-19 transmission and highlights its potential for informing effective pandemic management strategies. It also outlines promising avenues for further research to enhance our knowledge of COVID-19's impact on specific populations and inform future public health efforts.
International Journal of Public Health Science (IJPHS)

Received Jun 16, 2021 Revised Oct 30, 2021 Accepted Nov 12, 2021 The coronavirus disease 2019 (COVID-19) pandemic is continuing, but companies have been allowed to continue their business with the obligation strictly to apply health protocol. A cross-sectional study aimed to examine the perception of COVID-19 and its prevention behavior based on the health belief model was conducted on 285 industrial workers from eight company types sampled by online survey. The results show that most workers have poor COVID-19 perceptions (54.7%). However, the workers showing good behavior (61.1%) in preventing COVID-19 transmission. Gender (p=0.009), education background (p=0.017) and company types (p=0.001) were related to the COVID-19 perception. In addition, the perceptions related to the behavior in preventing COVID-19 transmission (p=0.000). Except cues to action, all perception components, i.e., perceived susceptibility (p=0.000), seriousness (p=0.005), benefits (p=0.000), barriers (p=0.000), and selfefficacy (p=0.000) related to behavior to prevent COVID-19 transmission. However, the perception components show a weak correlation with preventing behavior (r=0.167-0.234). The information types were related to perceived barriers (p=0.046) and cues to action (p=0.007). Maintain a safe distance (p=0.029), using a face mask (p=0.039), and eat nutritious food (p=0.019) related to information types. The increase of COVID-19 perception is the key to improve industrial workers' behavior to prevent the COVID-19 transmission.
Multiple linear regression model for the prediction of Changma onset date in Korea

In this research, the multiple linear regression models for the prediction of the Changma onset in Korea have been developed. The predictors are based on correlation analysis between Changma onset dates over 30 years (1973–2002), and the winds and geopotential heights at high, middle, and low levels of the troposphere during the preceding 6 months (December to May). The clearest correlation was observed at 850 hPa geopotential height field in the preceding April among 6 months. The fundamental meaning of this model indicates that Changma in Korea comes earlier when the Mascarene high becomes languished and the tropical storms around India and western North Pacific subtropical high (WNPSH) become strengthened. The reliability of this model has been tested and proved by statistical cross‐validation analysis, and additionally, has been analysed for differences in the 850 hPa stream flows for those years that Changma comes earlier and later. From the preceding April onwards, the Mascarene high tends to become weakened so that the cross‐equatorial flow is strengthened along the African east coast. The cross‐equatorial flow fortifies the tropical storms around Indian as it moves to the east. In the long run, the flow is influenced so that the WNPSH is more developed to the north, influencing Changma rain band to move north faster.
Development and characterization of EST-SSR markers for the Solidago virgaurea complex (Asteraceae) in the Japanese archipelago1

Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for the Solidago virgaurea complex, an ecologically and morphologically diverse species complex in the Japanese archipelago, to elucidate population genetic structure and examine taxonomic boundaries. Methods and Results: Utilizing the RNA sequencing data obtained by next-generation sequencing techniques, 15 polymorphic EST-SSR markers with three to 14 alleles were developed, most of which were transferable to different Solidago species native to Eurasia and North America. Conclusions: The EST-SSR markers developed in this study may be useful for elucidating the population structure and taxonomic delimitation of the species complex, as well as for investigating the population genetics and reproductive ecology of Solidago species.
A meta-analysis

Background: The relationship between MTHFR (5, 10-methylene tetrahydrofolate reductase) gene polymorphisms and Systemic Lupus Erythematosus (SLE) has been wildly studied, but the results are still conflicting. Therefore, the purpose of this meta and pooled analysis was to identify the role of the MTHFR SNP (single nucleotide polymorphism, rs1801133) in SLE in a large sample of subjects and to assess the risk of SLE. Methods: Data were collected from EMBASE, PubMed and China National Knowledge Infrastructure from inception to August, 2019. Summary odds ratio (OR) with 95% confidence interval (CI) was applied to assess the association. Subgroup and sensitivity analysis were performed to assess the potential sources of heterogeneity of the pooled estimation. Results:We identified seven eligible studies involving 882 cases and 991 controls. MTHFR rs1801133 T carrier was significantly associated with increased risk of SLE when comparing to C allele [ORs were 1.766 (1.014–3.075) for T carrier vs CC, P= . 04]. Furthermore, the results of the subgroup analysis by genotyping methods suggested that T allele significantly contributed to the risk of SLE for both by polymerase chain reaction-TaqMan (PCR-TaqMan) [10.111 (2.634–38.813) for TT vs CC, 3.467 (1.324–9.078) for CT vs CC and 3.744 (1.143–12.264) for TT vs C carrier]. Also the results of the subgroup analysis by ethnicity suggested that T allele significantly contributed to the risk of SLE for Asians [9.679 (4.444–21.082) for TT vs CC, 5.866 (3.021–11.389) for T carrier vs CC and 8.052 (3.861–16.795) for TT vs C carrier]. Conclusion: This cumulative meta-analysis showed that the MTHFR SNP (rs1801133) contributed to susceptibility of SLE. However, more multicentre well-designed case-control studies and larger sample sizes are exceedingly required to validate our findings in the future. Abbreviations: CI = confidence interval, CNKI = China National Knowledge Infrastructure, Hcy = Homocysteine, HWE = HardyWeinberg equilibrium, MAF = minor allele frequency, MTHFR = methylene tetrahydrofolate reductase, OR = odds ratio, PCC = population-based case-control controls, SLE = Systemic Lupus Erythematosus, SNP = single nucleotide polymorphism.
Cross-Cultural Adaptation and Psychometric Properties of the Traditional Chinese Version of the Italian Spine Youth Quality of Life (ISYQOL) Questionnaire

The Italian Spine Youth Quality of Life (ISYQOL) is a validated health-related quality of life (HRQOL) questionnaire for teenagers with adolescent idiopathic scoliosis (AIS). We culturally-adapted ISYQOL to traditional Chinese (ISYQOL-TC) and then recruited 133 conservatively treated teenagers with AIS to complete the ISYQOL-TC and the Chinese version of the Scoliosis Research Society-22 revised (SRS-22r) questionnaire, nine-item Patient Health Questionnaire (PHQ-9), seven-item Generalized Anxiety Disorder scale (GAD-7), and numeric pain rating scale (NPRS). They repeated ISYQOL-TC two weeks later. The internal consistency, unidimensionality, and test–retest reliability were measured using the Cronbach’s alpha, Rasch measurement models, and intra-class correlation coefficients (ICC3,1), respectively. The concurrent validity of the ISYQOL-TC with SRS-22r, and its construct validity with other questionnaires were evaluated using Spearman correlation coefficients. The ISYQOL-TC demonstrated good internal consistency (Cronbach’s alpha 0.90 and 0.89 for items 1–13 and items 1–20), and excellent test–retest reliability (ICC3,1 = 0.95–0.96). The Rasch analysis supported the unidimensionality of all 20 items in ISYQOL-TC. The ISYQOL-TC percentage scores were positively correlated with SRS-22r total scores (r = 0.65; p < 0.05), but were negatively related to PHQ-9, GAD-7, and NPRS scores (r = −0.46 to −0.39; p < 0.01). Collectively, the ISYQOL-TC is a reliable and valid instrument for evaluating HRQOL in Chinese teenagers with AIS.
The relationship between environmental exposures to phthalates and DNA damage in human sperm using the neutral comet assay.

Phthalates are industrial chemicals widely used in many commercial applications. The general population is exposed to phthalates through consumer products as well as through diet and medical treatments. To determine whether environmental levels of phthalates are associated with altered DNA integrity in human sperm, we selected a population without identiﬁed sources of exposure to phthalates. One hundred sixty-eight subjects recruited from the Massachusetts General Hospital Andrology Laboratory provided a semen and a urine sample. Eight phthalate metabolites were measured in urine by using high-performance liquid chromatography and tandem mass spectrometry; data were corrected for urine dilution by adjusting for speciﬁc gravity. The neutral single-cell microgel electrophoresis assay (comet assay) was used to measure DNA integrity in sperm. VisComet image analysis software was used to measure comet extent, a measure of total comet length (micrometers); percent DNA in tail (tail%), a measure of the proportion of total DNA pre-sent in the comet tail; and tail distributed moment (TDM), an integrated measure of length and intensity (micrometers). For an interquartile range increase in speciﬁc gravity–adjusted monoethyl phthalate (MEP) level, the comet extent increased signiﬁcantly by 3.6 µm [95% conﬁdence interval (95% CI), 0.74–6.47]; the TDM also increased 1.2 µm (95% CI, –0.05 to 2.38) but was of border-line signiﬁcance. Monobutyl, monobenzyl, monomethyl, and mono-2-ethylhexyl phthalates were not signiﬁcantly associated with comet assay parameters. In conclusion, this study represents the first human data to demonstrate that urinary MEP, at environmental levels, is associated with increased DNA damage in sperm
Application of Next-Generation Sequencing to Enterobacter Hormaechei Subspecies Analysis during a Neonatal Intensive Care Unit Outbreak

Introduction: The Enterobacter cloacae complex (ECC) species are potential neonatal pathogens, and ECC strains are among the most commonly encountered Enterobacter spp. associated with nosocomial bloodstream infections. Outbreaks caused by ECC can lead to significant morbidity and mortality in susceptible neonates. At the molecular level, ECC exhibits genomic heterogeneity, with six closely related species and subspecies. Genetic variability poses a challenge in accurately identifying outbreaks by determining the clonality of ECC isolates. This difficulty is further compounded by the limitations of the commonly used molecular typing methods, such as pulsed field gel electrophoresis, which do not provide reliable accuracy in distinguishing between ECC strains and can lead to incorrect conclusions. Next-generation sequencing (NGS) offers superior resolution in determining strain relatedness. Therefore, we investigated the clinical pertinence of incorporating NGS into existing bundle measures to enhance patient management during an outbreak of ECC in a level-3 neonatal intensive care unit (NICU) in Germany. Methods: As the standard of care, all neonates on the NICU received weekly microbiological swabs (nasopharyngeal and rectal) and analysis of endotracheal secretion, where feasible. During the 2.5-month outbreak, colonisation with ECC was detected in n = 10 neonates. The phylogenetic relationship and potential antimicrobial resistance genes as well as mobile genetic elements were identified via bacterial whole-genome sequencing (WGS) using Illumina MiSeq followed by in silico data analysis. Results: Although all ECC isolates exhibited almost identical antimicrobial susceptibility patterns, the WGS data revealed the involvement of four different ECC clones. The isolates could be characterised as Enterobacter hormaechei subspecies steigerwaltii (n = 6, clonal), subsp. hoffmannii (n = 3, two clones) and subsp. oharae (n = 1). Despite the collection of environmental samples, no source of this diffuse outbreak could be identified. A new standardised operating procedure was implemented to enhance the management of neonates colonised with MRGN. This collaborative approach involved both parents and medical professionals and successfully prevented further transmission of ECC. Conclusions: Initially, it was believed that the NICU outbreak was caused by a single ECC clone due to the similarity in antibiotic resistance. However, our findings show that antibiotic susceptibility patterns can be misleading in investigating outbreaks of multi-drug-resistant ECC. In contrast, bacterial WGS accurately identified ECC at the clonal level, which significantly helped to delineate the nature of the observed outbreak.