Miracle-dz/UniFAR-FTUs huggingface dataset:
Control of vacuole membrane homeostasis by a resident PI-3,5-kinase inhibitor

Significance The lysosome-like vacuole is the main organelle to degrade membrane proteins and organelles and, thus, provides amino acids, but also ions to the cytosol for cellular survival. Maintenance of vacuole membrane integrity is thus important for cellular adaptations. The vacuole contains several protein complexes on its surface to maintain the vacuole functional, and one such complex is a lipid kinase named Fab1 (of PIKfyve in human cells). Fab1 is part of a protein complex that produces a phosphorylated lipid, PI-3,5-P2. Other proteins bind PI-3,5-P2 and can fragment the vacuole to balance volume vs. membrane during stress. We now identify Ivy1 as a protein that binds Fab1 and controls its activity. Lysosomes have an important role in cellular protein and organelle quality control, metabolism, and signaling. On the surface of lysosomes, the PIKfyve/Fab1 complex generates phosphatidylinositol 3,5-bisphosphate, PI-3,5-P2, which is critical for lysosomal membrane homeostasis during acute osmotic stress and for lysosomal signaling. Here, we identify the inverted BAR protein Ivy1 as an inhibitor of the Fab1 complex with a direct influence on PI-3,5-P2 levels and vacuole homeostasis. Ivy1 requires Ypt7 binding for its function, binds PI-3,5-P2, and interacts with the Fab1 kinase. Colocalization of Ivy1 and Fab1 is lost during osmotic stress. In agreement with Ivy1’s role as a Fab1 regulator, its overexpression blocks Fab1 activity during osmotic shock and vacuole fragmentation. Conversely, loss of Ivy1, or lateral relocalization of Ivy1 on vacuoles away from Fab1, results in vacuole fragmentation and poor growth. Our data suggest that Ivy1 modulates Fab1-mediated PI-3,5-P2 synthesis during membrane stress and may allow adjustment of the vacuole membrane environment.
From microscopic to macroscopic sports injuries. Applying the complex dynamic systems approach to sports medicine: a narrative review

A better understanding of how sports injuries occur in order to improve their prevention is needed for medical, economic, scientific and sports success reasons. This narrative review aims to explain the mechanisms that underlie the occurrence of sports injuries, and an innovative approach for their prevention on the basis of complex dynamic systems approach. First, we explain the multilevel organisation of living systems and how function of the musculoskeletal system may be impaired. Second, we use both, a constraints approach and a connectivity hypothesis to explain why and how the susceptibility to sports injuries may suddenly increase. Constraints acting at multiple levels and timescales replace the static and linear concept of risk factors, and the connectivity hypothesis brings an understanding of how the accumulation of microinjuries creates a macroscopic non-linear effect, that is, how a common motor action may trigger a severe injury. Finally, a recap of practical examples and challenges for the future illustrates how the complex dynamic systems standpoint, changing the way of thinking about sports injuries, offers innovative ideas for improving sports injury prevention.
Three‐dimensional topography of corticopontine projections from rat sensorimotor cortex: Comparisons with corticostriatal projections reveal diverse integrative organization

The major cortical–subcortical re‐entrant pathways through the basal ganglia and cerebellum are considered to represent anatomically segregated channels for information originating in different cortical areas. A capacity for integrating unique combinations of cortical inputs has been well documented in the basal ganglia circuits but is largely undefined in the precerebellar circuits. To compare and quantify the amount of overlap that occurs in the first link of the cortico–ponto–cerebellar pathway, a dual tracing approach was used to map the spatial relationship between projections originating from the primary somatosensory cortex (SI), the secondary somatosensory cortex (SII), and the primary motor cortex (MI). The anterograde tracers biotinylated dextran amine and Fluoro‐Ruby were injected into homologous whisker representations of either SI and SII, or SI and MI. The ensuing pontine labeling patterns were analyzed using a computerized three‐dimensional reconstruction approach. The results demonstrate that whisker‐related projections from SI and MI are largely segregated. At some locations, the two projections are adjoining and partly overlapping. Furthermore, SI contributes significantly more corticopontine projections than MI. By comparison, projections from corresponding representations in SI and SII terminate in similar parts of the pontine nuclei and display considerable amounts of spatial overlap. Finally, comparison of corticopontine and corticostriatal projections in the same experimental animals reveals that SI–SII overlap is significantly larger in the pontine nuclei than in the neostriatum. These structural differences indicate a larger capacity for integration of information within the same sensory modality in the pontocerebellar system compared to the basal ganglia. J. Comp. Neurol. 478:306–322, 2004. © 2004 Wiley‐Liss, Inc.
Limits to grazing by herbivorous fishes and the impact of low coral cover on macroalgal abundance on a coral reef in Belize

Widespread rises in the abundance of fleshy macroalgae on Caribbean reefs within the last 20 yr have variously been attributed to eutrophication, overfishing, or the 1983-4 mass mortality of the grazing sea urchin Diadema antillarum. However, none of those factors can satisfactorily explain why macroalgae are abundant today even on lightly fished mid-depth reefs in sparsely pop- ulated areas. Here we explore another explanation for rises in macroalgal cover on such reefs, namely that they are a side effect of declines in coral cover. We suggest that grazing by herbivorous fishes can exclude macroalgae from mid-depth reefs with high cover of hard corals, but that on low- cover reefs, the amount of space occupied by algae overwhelms the ability of grazing fishes to crop it down. We simulated the effect of 10% and 25% rises in coral cover by attaching 'pseudo-corals' (PVC tiles covered in a non-toxic anti-fouling coating) to reef substratum in 5 × 5 m plots (3 groups: control, 10%T and 25%T) on a 12 m deep forereef site in front of Ambergris Caye, Belize. Within 3 mo of attaching tiles, macroalgae had declined by approximately 10% in 10%T plots and 25% in 25%T plots compared with control plots, and these new states persisted for the duration of the experiment (macro-algae averaging 38.0% in control plots, 28.7% in 10%T plots, and 13.6% in 25%T plots). Dra- matic reductions in macroalgae may have made the 25%T plots more attractive to herbivorous fishes, as, in the later stages of the experiment, herbivorous fish biomass and acanthurid feeding rate were higher in those plots than in 10%T and control plots.
A rights-based approach to sexuality education: conceptualization, clarification and challenges.

CONTEXT Although a rights-based approach to sexuality education has been increasingly discussed in the past decade, documented consensus regarding the goals, concepts and underlying assumptions of this approach is lacking. Differences in the assumed meaning of a rights-based approach can limit discussions of its implementation and evaluation, and impede opportunities to explore and critique a new model for sexuality education. METHODS In-depth interviews were conducted in 2012 with 21 U.S. and international sexuality education experts. Data were thematically coded and analyzed using an iterative approach. Responses were compared according to respondents' professional discipline and geographic focus. RESULTS A rights-based approach can be defined as the intersection of four elements: an underlying principle that youth have sexual rights; an expansion of programmatic goals beyond reducing unintended pregnancy and STDs; a broadening of curricula content to include such issues as gender norms, sexual orientation, sexual expression and pleasure, violence, and individual rights and responsibilities in relationships; and a participatory teaching strategy that engages youth in critical thinking about their sexuality and sexual choices. These elements were consistently identified by respondents across professional disciplines and geographic foci. In addition, all respondents raised questions about the feasibility of implementing a rights-based approach, particularly in the United States. CONCLUSIONS While questions remain to be answered regarding the implementation and impact of rights-based sexuality education, the proposed conceptual definition suggests multiple avenues for advocates, researchers, program developers and funders to enhance adolescent sexual health.
Neural processing of gendered information is more robustly associated with mothers’ gendered communication with children than mothers’ implicit and explicit gender stereotypes

ABSTRACT Processes like gender socialization (the ways in which parents convey information to their children about how girls and boys should behave) often happen unconsciously and might therefore be studied best with neuroscientific measures. We examined whether neural processing of gender-stereotype-congruent and incongruent information is more robustly related to mothers’ gendered socialization of their child than mothers’ implicit and explicit gender stereotypes. To this end, we examined event-related potentials (ERPs) of mothers (N = 35) completing an implicit gender-stereotype task and mothers’ gender stereotypes in relation to observed gendered communication with their child (2–6 years old) in a naturalistic picture-book-reading setting. Increased N2 activity (previously related to attentional processes) to gender stimuli in the implicit gender-stereotype task was associated with mothers’ positive evaluation of similar gendered behaviors and activities in the picture book they read with their child. Increased P300 activity (previously related to attention to unexpected events) to incongruent trials in the gender-stereotype task was associated with a more positive evaluation of congruent versus incongruent pictures. Compared to mothers’ gender stereotypes, neural processing of gendered information was more robustly related to how mothers talk to their children about boys’ and girls’ stereotype-congruent and incongruent behavior, and masculine and feminine activities.
Author ' s personal copy Scene congruency biases Binocular Rivalry

Contextual regularities, that is, objects’ tendency to appear with certain other objects, facilitate the processing of visual scenes and confer contextually incongruent objects with a special attentional status. This study was aimed at investigating the mechanisms underlying this attentional advantage using Binocular Rivalry (BR). In two experiments, congruent and incongruent images (e.g., a man drinking from a glass vs. a man ‘‘drinking’’ from a hairbrush) were pitted against each other, yielding a version of BR in which two objects rival within a given scene. Incongruent objects predominated in awareness longer than congruent ones. This effect stemmed from the fact that their dominance epochs lasted longer on the average than those of congruent objects, suggesting a difficulty to disengage attention from such objects. On the other hand, no support was found for the notion that incongruent objects also attract attention. 2011 Elsevier Inc. All rights reserved.
Cross-cultural adaptation and preliminary psychometric properties of the Affective Reactivity Index in Brazilian Youth: implications for DSM-5 measured irritability.

OBJECTIVE To describe the cross-cultural adaptation of the Affective Reactivity Index (ARI) to Brazilian Portuguese and to investigate preliminary psychometric properties of the adapted version. METHODS Cross-cultural adaptation was based on the investigation of the theoretical and operational equivalences of the original ARI in the Brazilian context, followed by a process of translation, back-translation, and review by a committee of experts. Data analysis was carried out in a community sample of 133 schoolchildren aged 8 to 17 years to investigate the following characteristics of the ARI: 1) factor structure; 2) internal consistency; 3) construct validity comparing differential relationships between irritability and anxiety dimensions and impairment; and 4) item response theory (IRT) parameters. RESULTS A final Brazilian Portuguese version of the instrument was defined and is presented. Internal consistency was good, and our analysis supported the original single-factor structure of the ARI. Correlations of the ARI with distress-related anxiety dimensions were higher than with phobic-related anxiety dimensions, supporting its construct validity. In addition, higher ARI scores were associated with higher irritability-related impairment. IRT analysis underscored frequency of loss of temper as essential to inform about pathological states of irritability. CONCLUSION The Brazilian Portuguese version of the ARI seems to be very similar to the original instrument in terms of conceptual, item, semantic, and operational equivalence. Our preliminary analysis replicates and extends previous evidence confirming promising psychometric properties for the ARI.
THE CENTRAL COMPONENT OF GRAVITATIONAL LENS Q0957+561

In 1981, a faint radio source (G′) was detected near the center of the lensing galaxy of the famous “twin quasar” Q0957+561. It is still unknown whether this central radio source is a third quasar image or an active nucleus of the lensing galaxy, or a combination of both. In an attempt to resolve this ambiguity, we observed Q0957+561 at radio wavelengths of 13 cm, 18 cm, and 21 cm, using the Very Long Baseline Array in combination with the phased Very Large Array and the Green Bank Telescope. We measured the spectrum of G′ for the first time and found it to be significantly different from the spectra of the two bright quasar images. This finding suggests that the central component is primarily or entirely emission from the foreground lens galaxy, but the spectrum is also consistent with the hypothesis of a central quasar image suffering free–free absorption. In addition, we confirm the previously-reported Very Long Baseline Interferometry position of G′ just north of the optical center of the lens galaxy. The position slightly favors the hypothesis that G′ originates in the lens, but is not conclusive. We discuss the prospects for further clarification of this issue.
Genetic diversity and Quaternary range dynamics in Iranian and Transcaucasian tortoises

1Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran 2Herpetology Section, Zoologisches Forschungsmuseum Alexander Koenig (ZFMK), Adenauerallee 160, 53113, Bonn, Germany 3Department of Zoology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, Ilkovičova 6, 842 15 Bratislava, Slovak Republic 4Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 61242 Brno, Czech Republic 5Museum of Zoology, Senckenberg Dresden, A. B. Meyer Building, 01109 Dresden, Germany 6Department of Biology, Faculty of Science, Bagabrisham 6714967346, Razi University, Kermanshah, Iran
Rapid Acoustic Model Adaptation Using Inverse MLLR-based Feature Generation

We propose a technique for generating a large amount of target speaker-like speech features by converting a large amount of prepared speech features of many speakers into features similar to those of the target speaker using a transformation matrix. To generate a large amount of target speaker-like features, the system only needs a very small amount of the target speaker’s utterances. This technique enables the system to adapt the acoustic model efficiently from a small amount of the target speaker’s utterances. To evaluate the proposed method, we prepared 100 reference speakers and 12 target (test) speakers. We conducted the experiments in an isolated word recognition task using a speech database collected by real PC-based distributed environments and compared our proposed method with MLLR, MAP and the method theoretically equivalent to the SAT. Experimental results proved that the proposed method needed a significantly smaller amount of the target speaker’s utterances than conventional M LLR, MAP and SAT.
Understanding Low Review Ratings in Online Communities: A Personality Based Approach

Online communities like Yelp thrive when users participate actively by writing good and useful reviews. While useful reviews are needed to keep the community active, understanding the users who post low rated and unhelpful reviews is also important, so that developers can implement persuasive strategies targeted at this group of users. In this paper, we identify those users who post low rated, unhelpful reviews and their personality types in Yelp using the Linguistic Inquiry and Word Count (LIWC) tool. The result of the analysis reveals that users who post unhelpful reviews are mostly of the personality type neuroticism. Using partial least squares structural equation modelling, we further explored the susceptibility of the different personality groups of users to rewards as a means of influencing them to write more useful reviews. Our results show that only the users that are high is extraversion who post unhelpful reviews are susceptible to rewards. This result demonstrates that rewards might not be persuasive to most of the Yelp users who post unhelpful reviews, hence the use of other persuasive strategies should be explored to influence users to post helpful reviews. The result of this study can be helpful to developers and stakeholders of online communities in implementing personalized influence strategies that work.
Multiple large-scale gene and genome duplications during the evolution of hexapods

Significance Polyploidy or whole genome duplication (WGD) is considered a major force in plant evolution, but less important in animals. The most diverse group of animals, the insects, are thought to have evolved without ever experiencing a WGD. Our analyses of diverse genomic data found evidence for 18 ancient WGDs and at least six other bursts of gene duplication during the evolution of insects. Further, we found that the functional patterns of gene retention and loss in these insect genomes are consistent with the signatures of WGDs. The expanding collection of genomic data for insects will provide many opportunities to further evaluate the evidence for WGDs and understand the impact of polyploidy on insect evolution. Polyploidy or whole genome duplication (WGD) is a major contributor to genome evolution and diversity. Although polyploidy is recognized as an important component of plant evolution, it is generally considered to play a relatively minor role in animal evolution. Ancient polyploidy is found in the ancestry of some animals, especially fishes, but there is little evidence for ancient WGDs in other metazoan lineages. Here we use recently published transcriptomes and genomes from more than 150 species across the insect phylogeny to investigate whether ancient WGDs occurred during the evolution of Hexapoda, the most diverse clade of animals. Using gene age distributions and phylogenomics, we found evidence for 18 ancient WGDs and six other large-scale bursts of gene duplication during insect evolution. These bursts of gene duplication occurred in the history of lineages such as the Lepidoptera, Trichoptera, and Odonata. To further corroborate the nature of these duplications, we evaluated the pattern of gene retention from putative WGDs observed in the gene age distributions. We found a relatively strong signal of convergent gene retention across many of the putative insect WGDs. Considering the phylogenetic breadth and depth of the insect phylogeny, this observation is consistent with polyploidy as we expect dosage balance to drive the parallel retention of genes. Together with recent research on plant evolution, our hexapod results suggest that genome duplications contributed to the evolution of two of the most diverse lineages of eukaryotes on Earth.
Parametric and semiparametric inference for shape: the role of the scale functional

We are considering the problem of efficient inference on the shape matrix of an elliptic distribution with unspecified location and either (a) fully specified radial density, (b) radial density specified up to a scale parameter, or (c) completely unspecified radial density. Bickel in [1] has shown that efficiencies under (b) and (c), while being strictly less than under (a), coincide: the efficiency loss caused by an unspecified radial density thus is entirely due to the non-specification of its scale (scale here is not necessarily measured by standard error, as second-order moments may be infinite). Defining scale however requires the choice of a particular scale functional, a choice which has an impact on efficiency bounds. We provide a closed form expression for this efficiency loss, both in hypothesis testing and in point estimation, as a function of the standardized radial density and the scale functional. We show that this loss is maximum at arbitrarily light tails whereas, under arbitrarily heavy tails, it is arbitrarily close to zero: hence, under very heavy tails, ignoring the scale (ignoring the exact density) asymptotically does not harm inference on shape. However, the same loss is nil, irrespective of the standardized radial density, when the scale functional (in dimension k) is the k-th root of the scatter determinant.
Taking advantage from phenotype variability in a local animal genetic resource: identification of genomic regions associated with the hairless phenotype in Casertana pigs.

Casertana is an endangered autochthonous pig breed (raised in south-central Italy) that is considered to be the descendant of the influential Neapolitan pig population that was used to improve British breeds in the 19th century. Casertana pigs are characterized by a typical, almost complete, hairless phenotype, even though a few Casertana pigs are normal haired. In this work, using Illumina PorcineSNP60 BeadChip data, we carried out a genome-wide association study and an FST analysis with this breed by comparing animals showing the classical hairless phenotype (n = 81) versus pigs classified as haired (n = 15). Combining the results obtained with the two approaches, we identified two significant regions: one on porcine chromosome (SSC) 7 and one on SSC15. The SSC7 region contains the forkhead box N3 (FOXN3) gene, the most plausible candidate gene of this region, considering that mutations in another gene of the same family (forkhead box N1; Foxn1 or FOXN1) are responsible for the nude locus in rodents and alopecia in humans. Another potential candidate gene, rho guanine nucleotide exchange factor 10 (ARHGEF10), is located in the SSC15 region. FOXN3 and ARHGEF10 have been detected as differentially expressed in androgenetic and senescent alopecia respectively. This study on an autochthonous pig breed contributes to shed some light on novel genes potentially involved in hair development and growth and demonstrates that local animal breeds can be valuable genetic resources for disclosing genetic factors affecting unique traits, taking advantage of phenotype variability segregating in small populations.