Miracle-dz/UniFAR-FTUs huggingface dataset:
nup154 Genetically Interacts With cup and Plays a Cell-Type-Specific Function During Drosophila melanogaster Egg-Chamber Development

Nucleoporin Nup154 is a Drosophila component of the nuclear pore complex (NPC), evolutionarily conserved from yeast to humans. While functional studies carried out in both yeast and metazoan cells indicated that Nup154 homologs are key elements of the NPC framework, the striking phenotypic specificity displayed by nup154 hypomorphic mutant alleles suggested that Nup154 might play additional roles in the context of the NPC. Actually, genetic analyses demonstrated that mutant nurse-cell nuclei do not undergo a normal chromosome dispersal process, uncovering an essential requirement for nup154 gene function during oogenesis. In this report, we show that Nup154 interacts genetically and physically with Cup, a germline-specific protein implicated in multiple aspects of female gametogenesis, including the regulation of the nurse-cell chromosome structure. The two proteins colocalize in vivo and are co-immunoprecipitated from ovarian extracts. Moreover, cup, nup154 double mutants exhibit much stronger oogenesis defects than single mutants. Our findings delineate an intriguing scenario where an ubiquitous nucleoporin might directly influence specialized developmental events.
If the antibody fails – a mass Western approach

Sucrose-phosphate synthase (SPS) has attracted the interest of plant scientists for decades. It is the key enzyme in sucrose metabolism and is under investigation in various plant species, e.g. spinach, tobacco, poplar, resurrection plants, maize, rice, kiwi and Arabidopsis thaliana. In A. thaliana, there are four distinct SPS isoforms. Their expression is thought to depend on environmental conditions and plant tissue. However, these data were derived from mRNA expression levels only. No data on SPS protein identification from crude extracts have been available until now. An antibody approach failed to distinguish the four isoforms. Therefore, we developed a method for SPS quantification and isoform-specific identification in A. thaliana complex protein samples. Samples were separated on SDS-PAGE, digested and directly applied to liquid chromatography/triple-stage quadrupole mass spectrometry (LC/TSQ-MS). In this approach, known as mass Western, samples were analysed in multi-reaction monitoring (MRM) mode, so that all four SPS isoforms could be measured in one experiment. In addition to the relative quantification, stable isotope-labelled internal peptide standards allowed absolute quantification of SPS proteins. Protein extracts from various plant tissues, samples harvested during the day or the night, and cold-stressed plants were analysed. The stress-specific SPS5a isoform showed increased concentrations in cold-stressed leaf material.
Simulation of resistance forces acting on surgical needles

Abstract High precision in the manual control of needles and biopsy probes in medical treatment requires high skill and dexterity levels. In anaesthesia, force sensation is an important feedback mechanism, and the practitioner needs to refresh or develop skills to improve on the interpretation of needle progress towards the target site. This paper describes an experimental tactile force simulator for uniaxial needle action for which the force resisting progress of the needle is derived from measured data. As an example, the approach taken to develop the simulation of the insertion of epidural needles is described. Adaptation to other procedures would be possible by adopting new reference models based on appropriate measured force data.
Serum antibodies to outer membrane proteins (OMPs) of Moraxella (Branhamella) catarrhalis in patients with bronchiectasis: identification of OMP B1 as an important antigen

Moraxella (Branhamella) catarrhalis is a common cause of lower respiratory tract infections in adults and of otitis media in children. Little is known about the human immune response to this bacterium. In this study, immunoblot assays were performed to detect serum immunoglobulin G antibodies directed at purified outer membrane of M. catarrhalis. Twelve serum samples, two each from six patients with bronchiectasis who were persistently colonized with this organism, were tested with their homologous M. catarrhalis sputum isolates. In all the sera, the most prominent and consistent antibody response was to a minor 84-kDa outer membrane protein, OMP B1. Immunoblot adsorption assays show that these antibodies recognize surface exposed epitopes on OMP B1. Further analysis of human serum antibodies eluted from the surface of intact bacterial cells shows that these surface-exposed epitopes on OMP B1 are heterogeneous among strains of M. catarrhalis. OMP B1 is therefore an important OMP antigen on the surface of M. catarrhalis for the human immune response to infection by this bacterium.
Does use of computer technology for perinatal data collection influence data quality?

Population health data, collected worldwide in an effort to monitor mortality and morbidity of mothers and babies, namely, perinatal data, are mandated at a federal level within Australia. The data are used to monitor patterns in midwifery, obstetric and neonatal practice, health outcomes, used for research purposes, funding allocation and education. Accuracy in perinatal data is most often reported via quantitative validation studies of perinatal data collections both internationally and in Australia. These studies report varying levels of accuracy and suggest researchers need to be more aware of the quality of data they use. This article presents findings regarding issues of concern identified by midwives relating to their perceptions of how technology affects the accuracy of perinatal data records. Perinatal data records are perceived to be more complete when completed electronically. However, issues regarding system functionality, the inconsistent use of terminology, lack of data standards and the absence of clear, written records contribute to midwives’ perceptions of the negative influence of technology on the quality of perinatal data.
Higher order structural effects stabilizing the reverse Watson–Crick Guanine-Cytosine base pair in functional RNAs

The G:C reverse Watson–Crick (W:W trans) base pair, also known as Levitt base pair in the context of tRNAs, is a structurally and functionally important base pair that contributes to tertiary interactions joining distant domains in functional RNA molecules and also participates in metabolite binding in riboswitches. We previously indicated that the isolated G:C W:W trans base pair is a rather unstable geometry, and that dicationic metal binding to the Guanine base or posttranscriptional modification of the Guanine can increase its stability. Herein, we extend our survey and report on other H-bonding interactions that can increase the stability of this base pair. To this aim, we performed a bioinformatics search of the PDB to locate all the occurencies of G:C trans base pairs. Interestingly, 66% of the G:C trans base pairs in the PDB are engaged in additional H-bonding interactions with other bases, the RNA backbone or structured water molecules. High level quantum mechanical calculations on a data set of representative crystal structures were performed to shed light on the structural stability and energetics of the various crystallographic motifs. This analysis was extended to the binding of the preQ1 metabolite to a preQ1-II riboswitch.
Effect of different truncation angles in spherical near-field measurements

In all antenna measurement scenarios the complete radiation pattern is not available or not measurable and this is mainly due to montage reasons, where the tower on which the antenna is mounted shadows the antenna's back side; causing loss of information. Other scenarios might be that the operator is not interested in the complete radiation pattern of the antenna but in some specific solid angle or area. In this paper truncation effect will be investigated, in which the missing field range will simply be filled with zeros. It will be shown that the location at which one truncates has a direct effect on the accuracy of the reconstructed antenna pattern in the valid region.
Voltage control strategies of low voltage distribution grids using photovoltaic systems

In this investigation, some strategies are presented for the optimized provision of reactive power by photovoltaic (PV) systems to perform voltage regulation in a low voltage distribution grid. The optimization is based on an optimal power flow algorithm that determines the minimum amount of reactive power needed for voltage regulation, also resulting in the minimization of losses in the network. In addition to solving overvoltage problems caused by high penetration of PV systems, reactive power exchange with the superimposed medium voltage grid is also minimized. This is accomplished by performing reactive power balance with respect to a power factor set-point at the interface of medium (MV) and low voltage (LV) grids. In one strategy, the benefits of voltage support provided by battery-based energy storage system (BESS) in parallel to PV systems are also presented.
Influence of the physical and psychological variables on physical injuries in football.

Over the years, a great number of studies have examined the importance of preventing sports injuries and developing effective physical procedures and psychological techniques for the recuperation of the injured athlete. The present study evaluates the effect of age, category, equipment, and anxiety/feature in the occurrence of injuries in football (soccer). A total of 277 players from 26 different football teams of Ciudad del Carmen (Mexico) participated in the study. Data on the variables of interest were compiled by the self-recording of sports injuries together with the questionnaire STAI-RASGO. The results did not reflect statistically significant differences between the variable sports injury and age, category, and anxiety/feature, respectively ( p =.542; p =.127; p =.598); significant differences were found only in the relation sport and team ( p < .001). We conclude that the cause of sports injuries is multifactorial and therefore interdisciplinary work is fundamental, such as the development of programmes that approach the athlete from an integral standpoint.
Exercise, fibrinogen, and other risk factors for ischaemic heart disease. Caerphilly Prospective Heart Disease Study.

OBJECTIVE--To examine the associations between physical activity and a wide range of risk factors for ischaemic heart disease including fibrinogen concentration and viscosity. DESIGN--Cross sectional evidence from the 2398 men aged 50-64 years in the Caerphilly Prospective Heart Disease Study. METHODS--Validated questionnaires were used to quantify energy expenditure on leisure activities and to grade activities related to occupation. Risk factors for heart disease examined included blood pressure, lipids, fibrinogen, and plasma viscosity. Possible confounding variables included smoking, employment, and prevalent heart disease (angina, previous myocardial infarction, and electrocardiographic evidence of ischaemia). RESULTS--Fibrinogen concentration was lower by 0.24 g/l and viscosity by 0.026 cP in the third of men who were most active in leisure activities (about 0.25 x 1 SD). A weak positive relation was found with high density lipoprotein cholesterol, but none with total cholesterol or fasting glucose concentrations or blood pressure. Triglyceride concentrations seem to be substantially lower in the most active men, although the evidence for this is not consistent. Work related activity showed relation with the lipid concentration but not with the haemostatic tests. CONCLUSIONS--Leisure activities of all levels seem to affect haemostatic and lipid factors beneficially. These effects correspond to a difference in the risk of heart disease for an active man and a sedentary man of at least 7% or 8%. Fasting triglyceride concentrations have already been shown to be strongly predictive of heart disease in this cohort of men, and the effect of exercise on this factor is also likely to confer benefit.
THE EVOLVING INTELLECTUAL DIVERSITY OF THE IS DISCIPLINE: EVIDENCE FROM REFERENT THEORETICAL FRAMEWORKS

For over 20 years, researchers expressed their concern for the lack of theoretical development in the Information Systems discipline and the lack of a cumulative research tradition. The aim of this study is to investigate the intellectual structure of the IS discipline through an analysis of 993 mainstream research articles published in selected journals and proceedings during the previous decade (1991-2000). We explore the variety of researcher perspectives in the discipline, based on the theoretical frameworks adopted by these studies. This examination shows the relative “mindshare” of different theoretical frameworks and informs the self-reflection that is frequently undertaken in our discipline. The study generates categorizations to map theories to frameworks, which are then located in a three-dimensional ontology. The resulting mappings should help researchers understand the scope of past IS research and identify gaps in theoretical development.
A Domesticated PiggyBac Transposase Interacts with Heterochromatin and Catalyzes Reproducible DNA Elimination in Tetrahymena

The somatic genome of the ciliated protist Tetrahymena undergoes DNA elimination of defined sequences called internal eliminated sequences (IESs), which account for ∼30% of the germline genome. During DNA elimination, IES regions are heterochromatinized and assembled into heterochromatin bodies in the developing somatic nucleus. The domesticated piggyBac transposase Tpb2p is essential for the formation of heterochromatin bodies and DNA elimination. In this study, we demonstrate that the activities of Tpb2p involved in forming heterochromatin bodies and executing DNA elimination are genetically separable. The cysteine-rich domain of Tpb2p, which interacts with the heterochromatin-specific histone modifications, is necessary for both heterochromatin body formation and DNA elimination, whereas the endonuclease activity of Tpb2p is only necessary for DNA elimination. Furthermore, we demonstrate that the endonuclease activity of Tpb2p in vitro and the endonuclease activity that executes DNA elimination in vivo have similar substrate sequence preferences. These results strongly indicate that Tpb2p is the endonuclease that directly catalyzes the excision of IESs and that the boundaries of IESs are at least partially determined by the combination of Tpb2p-heterochromatin interaction and relaxed sequence preference of the endonuclease activity of Tpb2p.
Changes in synaptic inputs to sympathetic preganglionic neurons after spinal cord injury

Spinal cord injury (SCI) leads to plastic changes in organization that impact significantly on central nervous control of arterial pressure. SCI causes hypotension and autonomic dysreflexia, an episodic hypertension induced by spinal reflexes. Sympathetic preganglionic neurons (SPNs) respond to SCI by retracting and then regrowing their dendrites within 2 weeks of injury. We examined changes in synaptic input to SPNs during this time by comparing the density and amino acid content of synaptic input to choline acetyltransferase (ChAT)‐immunoreactive SPNs in the eighth thoracic spinal cord segment (T8) in unoperated rats and in rats at 3 days or at 14 days after spinal cord transection at T4. Postembedding immunogold labeling demonstrated immunoreactivity for glutamate or γ‐aminobutyric acid (GABA) within presynaptic profiles. We counted the number of presynaptic inputs to measured lengths of SPN somatic and dendritic membrane and identified the amino acid in each input. We also assessed gross changes in the morphology of SPNs using retrograde labeling with cholera toxin B and light microscopy to determine the structural changes that were present at the time of evaluation of synaptic density and amino acid content. At 3 days after SCI, we found that retrogradely labeled SPNs had shrunken somata and greatly shortened dendrites. Synaptic density (inputs per 10‐μm membrane) decreased on ChAT‐immunoreactive somata by 34% but increased on dendrites by 66%. Almost half of the inputs to SPNs lacked amino acids. By 14 days, the density of synaptic inputs to dendrites and somata decreased by 50% and 70%, respectively, concurrent with dendrite regrowth. The proportion of glutamatergic inputs to SPNs in spinal cord‐transected rats (≈40%) was less than that in unoperated rats, whereas the GABAergic proportion (60–68%) increased. In summary, SPNs participate in vasomotor control after SCI despite profound denervation. An altered balance of excitatory and inhibitory inputs may explain injury‐induced hypotension. J. Comp. Neurol. 435:226–240, 2001. © 2001 Wiley‐Liss, Inc.
The effect of postings information on searching behaviour Bibliometrics and beyond : some thoughts on web-based citation analysis

The idea of a unified citation index to the literature of science was first outlined by Eugene Garfield [1] in 1955 in the journal Science. Science Citation Index has since established itself as the gold standard for scientific information retrieval. It has also become the database of choice for citation analysts and evaluative bibliometricians worldwide. As scientific publication moves to the web, and novel approaches to scholarly communication and peer review establish themselves, new methods of citation and link analysis will emerge to capture often liminal expressions of peer esteem, influence and approbation. The web thus affords bibliometricians rich opportunities to apply and adapt their techniques to new contexts and content: the age of ‘bibliometric spectroscopy’ [2] is dawning.
Design of Nonvolatile SRAM with Ferroelectric FETs for Energy-Efficient Backup and Restore

Nonvolatile SRAM (nvSRAM) has emerged as a promising approach to reducing the standby energy consumption by storing the state into an in situ nonvolatile memory element and shutting down the power supply. Existing nvSRAM solutions based on a nonvolatile backup in magnetic tunnel junction and ReRAM, however, are costly in backup and restore energy due to static current. This cost results in a long break-even time (BET) when compared with a lowered voltage standby volatile SRAM. This brief proposes an nvSRAM based on ferroelectric FETs (FeFETs) that are capable of fully avoiding such static current. A simple differential backup and restore circuitry is proposed, achieving sub-fJ/cell total energy per backup and restore operation at the 10-nm node. This leads to hundreds of times BET improvement over existing ReRAM nvSRAM solutions. This nvSRAM also indicates the future FeFET design trends for such memory-logic synergy.
Interactive technologies for autism

In meeting health, education, and lifestyle goals, technology can both assist individuals with autism, and support those who live and work with them, such as family, caregivers, coworkers, and friends. The uniqueness of each individual with autism and the context of their lives provide interesting design challenges for the successful creation and adoption of technologies for this domain. This Special Interest Group (SIG) aims to bring together those who study the use of technology by and for individuals with autism, those who design and de-velop new technologies, and those who are curious about getting involved. Areas that this SIG will consider include assistive technologies; tools for data collection and analysis; educational software; virtual reality rehabilitation environments; identifying users; need finding; user-centered collaborative design processes that include individuals who cannot speak or write; and product assessment. This SIG will provide opportunities for participants to join together and share their own pro-jects, design challenges, and lessons learned while fo-cusing on directions for future development. Those with experience and newcomers to the field are both equally encouraged to attend.
Dynamique des transferts intergénérationnels et effet de démonstration [Dynamics of Intergenerational Transfers and the Demonstration Effect]

In this paper, we extend the framework of the motives for familial solidarities to three generations. By focusing on upward transfers, we examine the demonstration effect hypothesis where individuals make transfers to their parents in order to be helped in return by their children. We show the limits of this model when the probability of imitation is exogenous. Then, we endogeneize this probability by assuming that imitative behaviors are infl uenced by the stock of upstream transfers made previously by the parents. The theoretical predictions are tested using two cross-sectional surveys from France. Using different types of upstream transfers, we fi nd little evidence in favor of the demonstration effect hypothesis.
Functional diversity within a morphologically conservative genus of predators: implications for functional equivalence and redundancy in ecological communities

Summary 1The idea that sets of species may have similar effects on population, community or ecosystem processes is a prevalent theme in many areas of ecology, especially in the context of biodiversity and ecosystem function. If indeed species are functionally equivalent, limiting similarity suggests that it should be closely related, morphologically similar species using similar resources in a similar manner. 2We assayed the functional equivalence of three congeneric, morphologically similar predatory fish species (genus Enneacanthus). Functional equivalence was evaluated using aspects of both effects of fish on a variety of prey responses and the growth responses of the fish themselves as a measure of energy consumption. Fish were matched by initial size to control for effects of body size. A strict definition of functional equivalence based on niche theory was used to delineate it from the alternative of functional diversity. 3Based on observed effects on larval anurans, only a single species pair could roughly be judged functionally equivalent, but these two species showed the greatest differences in growth rate and, hence, metabolic demand. Using the criterion of relative yield total, again, only a single pair could roughly be judged equivalent, however, members of this alternative species pair were dramatically different in their effects on larval anurans. Thus, as previously shown for a more diverse set of species, grouping of species by similarity in effects depends upon the specific response variable. 4Overall range of effects produced on a variety of response variables was surprising, given the similarity in morphology and autecology, strong phylogenetic affinity, and the fact that neither predator size nor growth explained significant variation. Each species appears to be interacting with the environment in a different manner, either as a consequence of differences in metabolic demand or differences in preferences or efficiency with regard to prey types. 5Observed responses are consistent with the predictions of niche theory and support an alternative explanation for observed relationships between diversity and ecosystem function. Our work suggests that functional equivalence may be uncommon, difficult to predict a priori, and that functional diversity, not functional equivalence, may underlie observed diversity–ecosystem function relationships.