Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of expression level of DNA repair-related genes involved in the NHEJ pathway on radiation-induced cognitive impairment

Cranial radiation therapy can induce cognitive decline. Impairments of hippocampal neurogenesis are thought to be a paramountly important mechanism underlying radiation-induced cognitive dysfunction. In the mature nervous system, DNA double-strand breaks (DSBs) are mainly repaired by non-homologous end-joining (NHEJ) pathways. It has been demonstrated that NHEJ deficiencies are associated with impaired neurogenesis. In our study, rats were randomly divided into five groups to be irradiated by single doses of 0 (control), 0 (anesthesia control), 2, 10, and 20 Gy, respectively. The cognitive function of the irradiated rats was measured by open field, Morris water maze and passive avoidance tests. Real-time PCR was also used to detect the expression level of DNA DSB repair-related genes involved in the NHEJ pathway, such as XRCC4, XRCC5and XRCC6, in the hippocampus. The influence of different radiation doses on cognitive function in rats was investigated. From the results of the behavior tests, we found that rats receiving 20 Gy irradiation revealed poorer learning and memory, while no significant loss of learning and memory existed in rats receiving irradiation from 0–10 Gy. The real-time PCR and Western blot results showed no significant difference in the expression level of DNA repair-related genes between the 10 and 20 Gy groups, which may help to explain the behavioral results, i.e. DNA damage caused by 0–10 Gy exposure was appropriately repaired, however, damage induced by 20 Gy exceeded the body's maximum DSB repair ability. Ionizing radiation-induced cognitive impairments depend on the radiation dose, and more directly on the body's own ability to repair DNA DSBs via the NHEJ pathway.
Interferon gamma, a mediator of lethal lipopolysaccharide-induced Shwartzman-like shock reactions in mice

The involvement of cytokines in the pathogenesis of a generalized, Shwartzman-like lethal inflammatory response to bacterial lipopolysaccharides (LPS) was studied by testing the ability of cytokines or neutralizing anticytokine antibodies to modify the course of the syndrome. The reaction was elicitable in non-SPF NMRI mice by two consecutive injections of S. marcescens LPS: a first injection in the footpad, followed after 24 h by an intravenous dose; the size and route of the preparatory LPS dose were found to be critical. Treatment with mAbs against IFN-gamma was found to completely prevent the reaction. Treatment with IFN-gamma on the other hand, rendered the mice more sensitive to elicitation of the reaction. In contrast, systemic administration of IFN-alpha/beta exerted a desensitizing effect. The role of endogenous cytokines in the pathogenesis of this generalized Shwartzman reaction was also documented by a study of the cytokine levels in the serum of the mice. In comparisons between mice given lethal and nonlethal induction schedules, a good correlation was found between mortality rates and height of IFN or TNF levels, but no correlation was seen with IL-6 levels. Also, in mice that were protected by anti-IFN-gamma antibody, serum IFN and TNF were undetectable, whereas IL-6 levels were as high as in unprotected mice. These data provide evidence that among the cytokines that govern the inflammatory response to LPS, endogenous IFN-gamma occupies a key position. These findings therefore also open perspectives for clinical application of IFN-gamma antagonists.
Sinusoidally modulated metasurface antenna excited by aperture coupling

A planar sinusoidally modulated reactive metasurface is excited through an aperture and a parametric analysis on the variation of its radiation properties is performed due to the variation in coupling coefficient of the aperture. The aperture coupling varies with the position and size of the aperture, the properties of the dielectric substrates and the propagating leaky-wave modes. The propagation of the fundamental leaky-wave mode is considered for this study.
Claudin-4 Undergoes Age-Dependent Change in Cellular Localization on Pig Jejunal Villous Epithelial Cells, Independent of Bacterial Colonization

Newborn piglets are immunologically naïve and must receive passive immunity via colostrum within 24 hours to survive. Mechanisms by which the newborn piglet gut facilitates uptake of colostral cells, antibodies, and proteins may include FcRn and pIgR receptor-mediated endocytosis and paracellular transport between tight junctions (TJs). In the present study, FcRn gene (FCGRT) was minimally expressed in 6-week-old gut and newborn jejunum but it was expressed at significantly higher levels in the ileum of newborn piglets. pIgR was highly expressed in the jejunum and ileum of 6-week-old animals but only minimally in neonatal gut. Immunohistochemical analysis showed that Claudin-5 localized to blood vessel endothelial cells. Claudin-4 was strongly localized to the apical aspect of jejunal epithelial cells for the first 2 days of life after which it was redistributed to the lateral surface between adjacent enterocytes. Claudin-4 was localized to ileal lateral surfaces within 24 hours after birth indicating regional and temporal differences. Tissue from gnotobiotic piglets showed that commensal microbiota did not influence Claudin-4 surface localization on jejunal or ileal enterocytes. Regulation of TJs by Claudin-4 surface localization requires further investigation. Understanding the factors that regulate gut barrier maturation may yield protective strategies against infectious diseases.
Cellular Immune Responses of Preterm Infants after Vaccination with Whole-Cell or Acellular Pertussis Vaccines

ABSTRACT Based on studies reporting specific antibody titers, it is recommended to vaccinate preterm infants against Bordetella pertussis according to their chronological age. However, as specific T-cell responses also are involved in the protection against B. pertussis, we have determined whether highly preterm infants (<31 weeks) are able to mount these immune responses during vaccination. Forty-eight premature infants were vaccinated at 2, 3, and 4 months of their chronological age with an acellular (Pa; n = 24) or a whole-cell (Pw; n = 24) tetravalent diphtheria-tetanus-pertussis-polio vaccine, and blood samples were collected at 2, 3, and 6 months of age. Most of the Pa- and Pw-vaccinated infants developed at 3 or 6 months of age a gamma interferon (IFN-γ) response to the B. pertussis antigens, accompanied by an interleukin-5 (IL-5) and IL-13 secretion for the Pa-vaccinated infants. No association was found between a very low infant birth weight, the occurrence of severe infections, and corticosteroid treatment or the administration of gammaglobulins with a low level of antigen-induced IFN-γ secretion. We conclude that like full-term infants, most preterm infants are able to mount a specific cellular immune response to the administration of the first doses of an acellular or a whole-cell pertussis vaccine.
Contextual proactivity for media sharing scenarios in proximity networks

Our mobile computing devices connect with other devices over proximity networks on a daily basis. Due to the availability of protocols like DLNA, applications in connected devices can enable media sharing experiences where content flows among devices in the proximity space. Despite the pervasiveness of DLNA and other similar implementations, average users perceive these connections to be intrinsically complex. This perception is not limited to media sharing and affects other types of N-device experiences. In this paper we propose to avoid the inherent complexity problem with the design of intelligent contextual systems that respond proactively to user intent. Users should be capable of expressing intent, and connected devices should gain the intelligence necessary to proactively satisfy user intent. We introduce a software service with an inference engine to determine when the contextual conditions are such that user intent can be satisfied. Media sharing connections, content, and protocols are proactively configured based on contextual inference in anticipation of user needs. The system has been designed as an Android service so that any media-sharing application that uses the service becomes contextually proactive.
ETB-QW InAs MOSFET with scaled body for improved electrostatics

This paper reports Extremely-Thin-Body (ETB) InAs quantum-well (QW) MOSFETs with improved electrostatics down to Lg = 50 nm (S =103 mV/dec, DIBL = 73 mV/V). These excellent metrics are achieved by using extremely thin body (1/3/1 nm InGaAs/InAs/InGaAs) quantum well structure with optimized layer design and a high mobility InAs channel. The ETB channel does not significantly degrade transport properties as evidenced by gm >1.5 mS/μm and vinj = 2.4 × 10 cm/s.
Development of inductive regional heating by energy control at frequency 2.45 GHz

This research focused on the adjustment of the heating distribution by controlling the energy supplied to the induction coil at the frequency of 2.45 GHz. The objective is to determine the area which the heat is uniformly distributed. The result from this research was believed to be effectively applied to the cancer treatment. The main equipment in the experiment was the induction coils which were sets of two coils and four coils, and each coil has its own power-supply unit. The result showed that the activation of supply units one at a time generated more heating area than the one when all units were activated at the same time. Moreover, the heating area depended on the number of coils and the temperature, so the understanding of these factors on the heat generation would significantly benefit the cancer treatment in the future.
A hybrid bootstrap filter for target tracking in clutter

The problem of tracking multiple targets with multiple sensors in the presence of interfering measurements is considered. A new hybrid bootstrap filter is proposed. The bootstrap filter is an approach where random samples are used to represent the target posterior distributions. By using this approach, we circumvent the usual problem of an exponentially increasing number of association hypotheses as well as allowing the use of any nonlinear/non-Gaussian system and/or measurement models.
Association analysis of the functional monoamine oxidase A gene promoter polymorphism in psychiatric disorders.

Functional characterization studies revealed that transcriptional activity of the human monoamine oxidase A (MAOA) gene is modulated by a polymorphic repetitive sequence located approximately 1.2 kb upstream of the ATG codon. To investigate the possible influence of the allelic variants of the MAOA gene-linked polymorphic region (MAOA-LPR) on the genetic predisposition to psychiatric disorders, we have performed a case-control association study. 174 patients with affective disorders and 258 patients with schizophrenia according to DSM-IV, as well as 229 population controls were tested. Statistical analysis showed no significant differences in allele or genotype frequencies between control and patient groups. Our results suggest that there is no association between MAOA-LPR genotype and susceptibility to recurrent major depression, bipolar disorder, and schizophrenia in our population.
Three-Element MIMO Antenna System With Pattern and Polarization Diversity for WLAN Applications

In this paper, a three-element compact multiple-input–multiple-output (MIMO) antenna system having both pattern and polarization (linear/circular) diversity is proposed. The proposed MIMO system consists of a chamfered-edge square patch antenna with an offset feed that provides circular polarization in broadside direction. Furthermore, two printed dipole antennas are placed adjacent to it for providing linearly polarized endfire radiation. The three-element MIMO antenna system exhibits good isolation (<bold>>15</bold> dB) without the use of any separate decoupling structure. The match between the simulated and measured results on fabricated antenna prototype suggests that the proposed antenna can be a good candidate for pattern-and polarization-diversity MIMO applications in the <inline-formula><tex-math notation="LaTeX">$\mathbf {5.8}$</tex-math></inline-formula>-GHz WLAN frequency range.
Hearing Aid Benefit in Patients with Mild Sensorineural Hearing Loss: A Systematic Review.

BACKGROUND Untreated sensorineural hearing loss (SNHL) is associated with chronic health-care conditions, isolation, loneliness, and reduced quality of life. Although hearing aids can minimize the negative effects of SNHL, only about one in five persons with SNHL seeks help for communication problems. Many persons wait 10 yr or more from the time they first notice a problem before pursuing amplification. Further, little information about the benefits of amplification is available for persons with mild SNHL (MSNHL), who likely defer treatment even longer. PURPOSE To conduct a systematic review to weigh the evidence regarding benefits derived from the use of amplification by adults with MSNHL. RESEARCH DESIGN Systematic review with meta-analysis. STUDY SAMPLE Adult hearing aid wearers with bilateral average pure-tone thresholds ≤45 dB HL at 500, 1000, 2000, and 4000 Hz. DATA COLLECTION AND ANALYSIS PubMed, Cumulative Index to Nursing and Allied-Health Literature, Cochrane Collaboration, and Google Scholar were searched independently by the authors during September 2013. The authors used a consensus approach to assess the quality and extract data for the meta-analysis. RESULTS Of 106 articles recovered for full-text review, only 10 met inclusion criteria (at least Level IV of evidence and involved and reported separate pre-/postfitting hearing aid outcomes for patients with MSNHL). Included studies involved mainly middle-aged to elderly patients using hearing aids of various styles and circuitry. Results from all of the studies indicated positive benefits from amplification for patients with MSNHL. Data from five studies were suitable for a meta-analysis, which produced a small-to-medium effect size of 0.85 (95% confidence intervals = 0.44-1.25) after adjusting for a small publication bias. This evidence confirmed benefits from the use of amplification in adults with MSNHL. CONCLUSIONS Evidence exists supporting the notion that adults with MSNHL benefit from hearing aids. This information is important and useful to audiologists, patients, and third-party payers, even considering that most of the studies in this systematic review were limited, somewhat dated, and used analog and early digital technology available when the studies were conducted. Clinical recommendations may be even stronger as future studies become available for patients fit with modern styles and high-technology hearing aids.
Effects of Antiepileptic Drugs on Refractory Seizures in the Intact Immature Corticohippocampal Formation In Vitro

Summary: Purpose: We developed a new in vitro preparation of immature rats, in which intact corticohippocampal formations (CHFs) depleted in magnesium ions become progressively epileptic. The better to characterize this model, we examined the effects of 14 antiepileptic drugs (AEDs) currently used in clinical practice.
Development of conceptual model in understanding the role of organizational factor in KMS acceptance

The aim of this study is to develop a conceptual model that could use to understanding the role of organizational factors in KMS acceptance. Theory Technology Acceptance Model (TAM) along with organizational factors are using in develop the conceptual model for this study. Study was conducted by collected and analysis relevant article in this area. Variable and indictors in the conceptual model was develop and modify to fit the need of this study. The Results of this study is the propose model to understand the role of organizational factors in KMS acceptance.
Analysis of Differences Between Spect Images of the Left and Right Cerebral Hemispheres in Patients with Epileptic Symptoms

A plastic, translucent lampshade comprises a tube with radially inwardly extending flanges at each end. A lens rests upon the lower flange, while a mounting bracket connects to two slots defined in the upper flange to support the lampshade from the ceiling. The surface of the tube is smooth so that decorative self-adhesive paper can be attached.
Sport Support: Received Social Support as a Predictor of Athlete Satisfaction

Researchers have advocated for the exploration of communicative and psychological aspects of athlete-coach relationships associated with positive outcomes for athletes such as satisfaction. A particularly salient construct within literature that leads to satisfaction across contexts is receiving social support. This study examined 216 athletes’ reports of social support (i.e., tangible, informational, emotional, and esteem) received from head coaches, as well as their satisfaction with coaches and sport experiences. Results indicated that linear combinations of social support received from head coaches predicted athletes’ satisfaction with coaches and sport experiences. Specifically, informational, emotional, and esteem support accounted for unique variance in athletes’ satisfaction with coaches, whereas informational and emotional support accounted for unique variance in athletes’ satisfaction with sport experiences. Mediation tests revealed that the relationships between receiving social support and satisfaction with sport experiences were mediated by athletes’ satisfaction with coaches. These findings have implications for the continued examination of prosocial athlete-coach communication.
Supporting Collaboration Awareness with Real-Time Visualization of Development Activity

In the context of multi-developer projects, where several people are contributing code, developers must deal with concurrent development. Collaboration among developers assumes a fundamental role, and failing to address it can result, for example, in shipping delays. We argue that tool support for collaborative software development augments the level of awareness of developers, and consequently, help them to collaborate and coordinate their activities. In this context, we present an approach to augment awareness by recovering development information in real time and broadcasting it to developers in the form of three lightweight visualizations. Scamp, the Eclipse plug-in supporting this, is part of our Syde tool to support collaboration. We illustrate the usage of Scamp in the context of two multi-developer projects.
Cree encephalitis is allelic with Aicardi-Goutiéres syndrome: implications for the pathogenesis of disorders of interferon alpha metabolism

Aicardi-Goutiéres syndrome (AGS) is an early onset, progressive encephalopathy characterised by calcification of the basal ganglia, white matter abnormalities, and a chronic cerebrospinal fluid (CSF) lymphocytosis. Cree encephalitis shows phenotypic overlap with AGS although the conditions have been considered distinct because of immunological abnormalities observed in Cree encephalitis. We report that levels of interferon alpha (IFN-α), a marker of AGS, are raised in Cree encephalitis. Moreover, linkage analysis indicates that the disorders are allelic and refines the AGS1 locus to a 3.47 cM critical interval. Our data show that a CSF lymphocytosis is not necessary for the diagnosis of AGS and strongly suggest that AGS and pseudo-TORCH syndrome are the same disorder. Recognition of immunological dysfunction as part of the AGS phenotype provides further evidence of a primary pathogenic role for abnormal IFN-α production in AGS.
Mechanism of interaction of Dictyostelium severin with actin filaments

Severin, a 40,000-dalton protein from Dictyostelium that disassembles actin filaments in a Ca2+ -dependent manner, was purified 500-fold to greater than 99% homogeneity by modifications of the procedure reported by Brown, Yamamoto, and Spudich (1982. J. Cell Biol. 93:205-210). Severin has a Stokes radius of 29 A and consists of a single polypeptide chain. It contains a single methionyl and five cysteinyl residues. We studied the action of severin on actin filaments by electron microscopy, viscometry, sedimentation, nanosecond emission anisotropy, and fluorescence energy transfer spectroscopy. Nanosecond emission anisotropy of fluoresence-labeled severin shows that this protein changes its conformation on binding Ca2+. Actin filaments are rapidly fragmented on addition of severin and Ca2+, but severin does not interact with actin filaments in the absence of Ca2+. Fluorescence energy transfer measurements indicate that fragmentation of actin filaments by severin leads to a partial depolymerization (t1/2 approximately equal to 30 s). Depolymerization is followed by exchange of a limited number of subunits in the filament fragments with the disassembled actin pool (t1/2 approximately equal to 5 min). Disassembly and exchange are probably restricted to the ends of the filament fragments since only a few subunits in each fragment participate in the disassembly or exchange process. Steady state hydrolysis of ATP by actin in the presence of Ca2+-severin is maximal at an actin: severin molar ratio of approximately 10:1, which further supports the inference that subunit exchange is limited to the ends of actin filaments. The observation of sequential depolymerization and subunit exchange following the fragmentation of actin by severin suggests that severin may regulate site-specific disassembly and turnover of actin filament arrays in vivo.
Neuroscience-Inspired Dynamic Architectures

Neuroscience-inspired computational elements and architectures are one of the most popular ideas for replacing the von Neumann architecture. In this work, we propose a neuroscience-inspired dynamic architecture (NIDA) and discuss a method for automatically designing NIDA networks to accomplish tasks. We discuss the reasons we chose evolutionary optimization as the main design method and propose future directions for the
The 3′ Untranslated Region of Tumor Necrosis Factor Alpha mRNA Is a Target of the mRNA-Stabilizing Factor HuR

ABSTRACT Posttranscriptional regulation is important for tumor necrosis factor alpha (TNF-α) expression in monocytes and macrophages, and an AU-rich element (ARE) in the 3′ untranslated region (UTR) of TNF-α mRNA is implicated in control of its translation and mRNA stability. Regulation of mRNA turnover is thought to be mediated bytrans-acting proteins, which bind the ARE and stabilize or destabilize the transcript. However, with the exception of the destabilizing factor tristetraprolin, the identity and function of the proteins binding the TNF-α mRNA ARE have not been established. To identify other proteins involved in the posttranscriptional control of TNF-α, the subcellular location of TNF-α mRNA was determined in the macrophage-like cell line RAW 264.7. TNF-α mRNA was located in the pellet following centrifugation of cytoplasm at 100,000 ×g (P100 fraction). This fraction also contained proteins which formed two distinct ARE-specific complexes with the TNF-α mRNA 3′ UTR in electrophoretic mobility shift assays (EMSAs). A protein present in these two complexes was purified and identified by peptide mass mapping and tandem mass spectrometry as HuR. In EMSAs both complexes were supershifted by an anti-HuR antibody, while Western blotting also demonstrated the presence of HuR in the P100 extract. A HeLa cell tetracycline-regulated reporter system was used to determine the effect of HuR on mRNA stability. In this system, overexpression of HuR resulted in stabilization of an otherwise unstable reporter-mRNA containing the TNF-α ARE. These results demonstrate that the TNF-α ARE is a target of the mRNA-stabilizing factor HuR.