Miracle-dz/UniFAR-FTUs huggingface dataset:
The embryonic form of neural cell surface molecule (E-NCAM) in the rat hippocampus and its reexpression on glial cells following kainic acid- induced status epilepticus

The neural cell adhesion molecule (NCAM) changes at the cell surface during development, from highly sialylated forms (embryonic or E-NCAM) to three size classes of less sialylated proteins with apparent molecular mass of 180, 140, and 120 kDa (adult NCAM). In the nervous system, E-NCAM has been localized in developing tissues, where it is thought to play a role in the structuring of neuronal groups and tissue pattern formation. In the present study a monoclonal antibody that specifically detects E-NCAM was used in immunoblot and immunohistochemical procedures. In developing rat hippocampus, E-NCAM cell expression was found to change according to a precise pattern and persisted until 1 month after birth. It was closely associated with the mossy fiber system, an area known for its sprouting propensity. In adult rats, although immunoreactivity considerably decreases and becomes undetectable by immunoblot analysis, E-NCAM was still found to be associated with a few pyramidal-shaped cells in the innermost part of the dentate gyrus. In order to acquire some insight into potential histogenetically plastic functions of E-NCAM, in another series of experiments adult rats were treated with kainic acid, a powerful excitotoxic and convulsant glutamate analog eliciting status epilepticus. When these animals were examined for E-NCAM expression, an intense labeling was found associated with glial-like cells, particularly in the hippocampal formation, and corresponding approximately to the reactive gliosis, as confirmed by staining with anti-glial fibrillary acidic protein antibodies. This expression was detectable from about 3 d following kainic acid administration and persisted for at least 12 weeks; it developed according to an observable spatiotemporal distribution pattern. In animals submitted to amygdala kindling, a nonlesional model of secondarily generalized epilepsy, no such reexpression of E-NCAM was observed. Our observations imply that polysialylation may be a means of identifying neuronal structures capable of plasticity in the CNS. Moreover, intense reexpression of E-NCAM could be a marker of reactive gliosis following brain damage.
High level synthesis for data-driven applications

John von Neumann proposed his famous architecture in a context where hardware was very expensive and bulky. His goal was to maximize functionality with minimal hardware. Presently, logical gates are nearly free and single chips contain billions of gates. However, most current designs are still based on Von Neumann's architecture because processors are built on this model. Nevertheless, the main current challenge is to be able to design, refine, synthesize and verify new architectures in a minimum time and with a maximum computational performance regardless of the gate count. Data driven architectures enable a high level of parallelism because instead of a single controller managing all the resources (and often a single ALU), tens or hundreds of small controllers can now operate in parallel on local processing units. This paper presents an environment for the high level description, refinement, synthesis and verification of such systems. Our own HDL is presented with its compiler and we show how it can be used as the intermediate language of a compiler for an even higher level functional programming language. Ongoing work enables the interfacing with other languages (from both hardware and software communities). We also intend to target asynchronous designs.
Parameterized mixed cluster editing via modular decomposition

In this paper we introduce a natural generalization of the well-known problems Cluster Editing and Bicluster Editing, whose parameterized versions have been intensively investigated in the recent literature. The generalized problem, called Mixed Cluster Editing or ${\cal M}$-Cluster Editing, is formulated as follows. Let ${\cal M}$ be a family of graphs. Given a graph $G$ and a nonnegative integer $k$, transform $G$, through a sequence of at most $k$ edge editions, into a target graph $G'$ with the following property: $G'$ is a vertex-disjoint union of graphs $G_1, G_2, \ldots$ such that every $G_i$ is a member of ${\cal M}$. The graph $G'$ is called a mixed cluster graph or ${\cal M}$-cluster graph. Let ${\cal K}$ denote the family of complete graphs, ${\cal KL}$ the family of complete $l$-partite graphs ($l \geq 2$), and $\L={\cal K} \cup {\cal KL}$. In this work we focus on the case ${\cal M} = {\cal L}$. Using modular decomposition techniques previously applied to Cluster/Bicluster Editing, we present a linear-time algorithm to construct a problem kernel for the parameterized version of ${\cal L}$-Cluster Editing. Keywords: bicluster graphs, cluster graphs, edge edition problems, edge modification problems, fixed-parameter tractability, NP-complete problems.
Manufacturing and Performance Evaluation of Polyurethane Composites Using One-Part and Two-Part Resin Systems

Polyurethane (PU)-based composites show superior performance compared to polyester and vinylester composites. The demand for PU composites is increasing in high technology as well as conventional applications such as infrastructure and automobile. In this study, glass fiber reinforced composite laminates using one-part and two-part PU resin are manufactured using vacuum-assisted resin transfer molding (VARTM) process. A new generation two-part thermoset PU resin system is investigated and compared with commercial one-part PU resin systems. The mechanical performance of glass fiber reinforced composites manufactured using two different PU resin systems is evaluated. Tensile and flexure tests are conducted on both neat resin and glass/PU composites. Low-velocity impact tests are performed on both types of glass fiber reinforced PU composite specimens. Mechanical properties including strength and modulus were measured and analyzed for the two resin systems. Differential scanning calorimetry (DSC) is used to study the cure behavior of both resin systems. A Brookfield LVDV-II programmable rotational type viscometer is employed to study the viscosity profiles of the resin systems. The influence of resin properties on the overall performance of glass fiber reinforced composites is discussed.
Identification of a rice zinc finger protein whose expression is transiently induced by drought, cold but not by salinity and abscisic acid

A C2H2-type zinc finger protein gene ZFP245 was cloned by RT-PCR approach from cold treated rice seedlings. ZFP245 is 712 bp in length and encodes a 24.5 KDa protein, which has 35% identity in amino acid with SCOF-1, a cold-inducible zinc finger protein from soybean. By database search, ZFP245 was mapped on chromosome 7 and clustered together with another C2H2 zinc finger gene. Tissue expression analysis showed that ZFP245 was constitutively expressed in various rice tissues including roots, stems, leaves and spikes. The semi-quantitative-RT-PCR assay revealed ZFP245 was strongly induced after 6 h exposure to cold and drought stresses, and then reduced to the baseline. However, ZFP245 was not regulated by high salt or abscisic acid treatment. The promoter sequence of 1017 nucleotides, upstream of ZFP245, was cloned directly by PCR approach. Sequence analysis revealed a CRT/DRE element was found within the ZFP245 promoter region. Taken together, ZFP245, as the first identified C2H2-type zinc finger protein involved in stress response in monocots probably plays a role as a transcription regulator in plant cold and drought responses through an ABA-independent pathway.
Elucidating the Role of Effectors in Plant-Fungal Interactions: Progress and Challenges

Pathogenic fungi have diverse growth lifestyles that support fungal colonization on plants. Successful colonization and infection for all lifestyles depends upon the ability to modify living host plants to sequester the necessary nutrients required for growth and reproduction. Secretion of virulence determinants referred to as “effectors” is assumed to be the key governing factor that determines host infection and colonization. Effector proteins are capable of suppressing plant defense responses and alter plant physiology to accommodate fungal invaders. This review focuses on effector molecules of biotrophic and hemibiotrophic plant pathogenic fungi, and the mechanism required for the release and uptake of effector molecules by the fungi and plant cells, respectively. We also place emphasis on the discovery of effectors, difficulties associated with predicting the effector repertoire, and fungal genomic features that have helped promote effector diversity leading to fungal evolution. We discuss the role of specific effectors found in biotrophic and hemibiotrophic fungi and examine how CRISPR/Cas9 technology may provide a new avenue for accelerating our ability in the discovery of fungal effector function.
Smoking Ban and Small-For-Gestational Age Births in Ireland

Background Ireland introduced a comprehensive workplace smoke-free legislation in March, 2004. Smoking-related adverse birth outcomes have both health care and societal cost implications. The main aim of this study was to determine the impact of the Irish smoke-free legislation on small-for-gestationa- age (SGA) births. Methods and Findings We developed a population-based birthweight (BW) percentile curve based on a recent study to compute SGA (BW <5th percentile) and very SGA (vSGA - BW<3rd percentile) for each gestational week. Monthly births born between January 1999 and December 2008 were analyzed linking with monthly maternal smoking rates from a large referral maternity university hospital. We ran individual control and CUSUM charts, with bootstrap simulations, to pinpoint the breakpoint for the impact of ban implementation ( = April 2004). Monthly SGA rates (%) before and after April 2004 was considered pre and post ban period births, respectively. Autocorrelation was tested using Durbin Watson (DW) statistic. Mixed models using a random intercept and a fixed effect were employed using SAS (v 9.2). A total of 588,997 singleton live-births born between January 1999 and December 2008 were analyzed. vSGA and SGA monthly rates declined from an average of 4.7% to 4.3% and from 6.9% to 6.6% before and after April 2004, respectively. No auto-correlation was detected (DW = ∼2). Adjusted mixed models indicated a significant decline in both vSGA and SGA rates immediately after the ban [(−5.3%; 95% CI −5.43% to −5.17%, p<0.0001) and (−0.45%; 95% CI: −0.7% to −0.19%, p<0.0007)], respectively. Significant gradual effects continued post the ban periods for vSGA and SGA rates, namely, −0.6% (p<0.0001) and −0.02% (p<0.0001), respectively. Conclusions A significant reduction in small-for-gestational birth rates both immediately and sustained over the post-ban period, reinforces the mounting evidence of the positive health effect of a successful comprehensive smoke-free legislation in a vulnerable population group as pregnant women.
Purification and characterization of galanin and scyliorhinin I from the hybrid sturgeon, Scaphirhynchus platorynchus x Scaphirhynchus albus (Acipenseriformes).

The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu10-Leu-Gly-Pro-His-Ala-Val -As p-Gly-His-Arg20-Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro.NH2) contains only two amino acid substitutions (Ser23 --> Asn and Pro29 --> Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met.NH2) contains only one amino acid substitution (Tyr3 --> Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
Training SpamAssassin with Active Semi-supervised Learning

Most spam filters include some automatic pattern classifiers based on machine learning and pattern recognition techniques. Such classifiers often require a large training set of labeled emails to attain a good discriminant capability between spam and legitimate emails. In addition, they must be frequently updated because of the changes introduced by spammers to their emails to evade spam filters. To address this issue active learning and semi-supervised learning techniques can be used. Many spam filters allow the user to give a feedback on personal emails automatically labeled during filter operation, and some filters include a self-training mechanism to exploit the large number of unlabeled emails collected during filter operation. However, users are usually willing to label only a few emails, and the benefits of selftraining techniques are limited. In this paper we propose an active semi-supervised learning method to better exploit unlabeled emails, which can be easily implemented as a plug-in in real spam filters. Our method is based on clustering unlabeled emails, querying the label of one email per cluster, and propagating such label to the most similar emails of the same cluster. The e! ectiveness of our method is evaluated using the well known open source SpamAssassin filter, on a large and publicly available corpus of real legitimate and spam emails.
A Stochastic Knapsack Queuing Model for Capacity Planning in TD-SCDMA System

Stochastic Knapsack Queuing Model (SKQM) can be applied to estimate the network capacity of a telecommunication network where different services are transmitted under various QoS constraints. However, the serious scrutiny and refinement are required before the original model can be fitted into the real-world network deployment. In this paper, we start from the assumption, principle, proof and theoretical result of our improved stochastic knapsack queuing model and apply it to tackle real-world network capacity planning problem in TD-SCDMA system. We develop a general network planning procedure based on SKQM in TD-SCDMA and prove its effectiveness through the case study. Numerical results show that the proposed SKQM have a good performance in capacity planning in TD-SCDMA.
Progression of pleural and parenchymal disease on chest radiographs of workers exposed to amosite asbestos.

OBJECTIVES: To determine predictors of progression of pleural and parenchymal disease on the chest radiographs of workers exposed to a short term, intense exposure of amosite asbestos. METHODS: The first and last of a series of chest radiographs of 887 workers exposed to amosite was interpreted and coded according to International Labour Organisation (ILO) standards by two physicians. Significant predictors of disease progression were found by a linear stepwise regression analysis from among such variables as smoking history, latency (time since first exposure), duration and intensity of exposure, and cytology. RESULTS: Although most radiographs remained normal, some showed progression of disease with about twice as many patients with abnormalities on the last film. Various combinations of age, intensity of exposure, and time between films were significant predictors of pleural and parenchymal disease and progression of such disease. No predominance of one sided disease was noted. Cytology and smoking were unreliable predictors of disease. Most disease progression was minor, usually of less than two scoring categories. CONCLUSION: An intense, yet short, exposure to amosite asbestos can produce pleural and parenchymal changes on chest radiographs. The number of those affected roughly doubled over a period spanning 10 to 20 years after exposure. Age and intensity of exposure are the most important predictors of disease.
The Receptor for Advanced Glycation End Products (RAGE) and the Lung

The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily of cell surface molecules. As a pattern-recognition receptor capable of binding a diverse range of ligands, it is typically expressed at low levels under normal physiological conditions in the majority of tissues. In contrast, the lung exhibits high basal level expression of RAGE localised primarily in alveolar type I (ATI) cells, suggesting a potentially important role for the receptor in maintaining lung homeostasis. Indeed, disruption of RAGE levels has been implicated in the pathogenesis of a variety of pulmonary disorders including cancer and fibrosis. Furthermore, its soluble isoforms, sRAGE, which act as decoy receptors, have been shown to be a useful marker of ATI cell injury. Whilst RAGE undoubtedly plays an important role in the biology of the lung, it remains unclear as to the exact nature of this contribution under both physiological and pathological conditions.
The Two-Component Regulatory System VicRK is Important to Virulence of Streptococcus equi Subspecies equi

This study aims at evaluating the importance of the two-component regulatory system VicRK to virulence of the horse pathogen Streptococcus equi subspecies equi and the potential of a vicK mutant as a live vaccine candidate using mouse infection models. The vicK gene was deleted by gene replacement. The ΔvicK mutant is attenuated in virulence in both subcutaneous and intranasal infections in mice. ΔvicK grows less slowly than the parent strain but retains the ability of S. equi to resist to phagocytosis by polymorphoneuclear leukocytes, suggesting that the vicK deletion causes growth defect. ΔvicK infection protects mice against reinfection with a wild-type S. equi strain. Intranasal ΔvicK infection induces production of anti-SeM mucosal IgA and systemic IgG. These results indicate that VicRK is important to S. equi growth and virulence and suggest that ΔvicK has the potential to be developed as a live S. equi vaccine.
Stability and Morphology Comparisons of Self-AssembledVirus-Like Particles from Wild-Type and Mutant Human Hepatitis B VirusCapsidProteins

ABSTRACT Instead of displaying the wild-type selective export of virions containing mature genomes, human hepatitis B virus (HBV) mutant I97L, changing from an isoleucine to a leucine at amino acid 97 of HBV core antigen (HBcAg), lost the high stringency of selectivity in genome maturity during virion export. To understand the structural basis of this so-called “immature secretion” phenomenon, we compared the stability and morphology of self-assembled capsid particles from the wild-type and mutant I97L HBV, in either full-length (HBcAg1-183) or truncated core protein contexts (HBcAg1-149 and HBcAg1-140). Using negative staining and electron microscopy, full-length particles appear as “thick-walled” spherical particles with little interior space, whereas truncated particles appear as“ thin-walled” spherical particles with a much larger inner space. We found no significant differences in capsid stability between wild-type and mutant I97L particles under denaturing pH and temperature in either full-length or truncated core protein contexts. In general, HBV capsid particles (HBcAg1-183, HBcAg1-149, and HBcAg1-140) are very robust but will dissociate at pH 2 or 14, at temperatures higher than 75°C, or in 0.1% sodium dodecyl sulfate (SDS). An unexpected upshift banding pattern of the SDS-treated full-length particles during agarose gel electrophoresis is most likely caused by disulfide bonding of the last cysteine of HBcAg. HBV capsids are known to exist in natural infection as dimorphic T=3 or T=4 icosahedral particles. No difference in the ratio between T=3 (78%) and T=4 particles (20.3%) are found between wild-type HBV and mutant I97L in the context of HBcAg1-140. In addition, we found no difference in capsid stability between T=3 and T=4 particles successfully separated by using a novel agarose gel electrophoresis procedure.
LARGELARGE PapierPapier n ° 81 Does Collateral Help Mitigate Adverse Selection ? A Cross-Country Analysis

We investigate whether collateral helps to solve adverse selection problems. Theory predicts a negative relationship between presence of collateral and risk premium, as collateral constitutes a signalling instrument for the borrower to be charged with a lower risk premium. However, bankers’ view and most empirical evidence contradict this prediction in accordance with the observed-risk hypothesis. We provide new evidence with loan-level data and country-level data for a sample of 5843 bank loans from 43 countries. We test whether the degree information asymmetries affects the link between the presence of collateral and risk premium. We include five proxies for the degree of information asymmetries, measuring opacity of financial information, trust, and development. We find that a greater degree of information asymmetries reduces the positive relationship between the presence of collateral and the risk premium. This finding provides support for the adverse selection and observed-risk hypotheses, as both hypotheses may be empirically validated depending of the degree of information asymmetries in the country.
Patient-Specific Dose and Radiation Risk Estimation in Pediatric Cardiac Catheterization

Background—Because of the higher radiosensitivity of infants and children compared with adults, there is a need to evaluate the doses delivered to pediatric patients who undergo interventional cardiac procedures. However, knowledge of the effective dose in pediatric interventional cardiology is very limited. Methods and Results—For an accurate risk estimation, a patient-specific Monte Carlo simulation of the effective dose was set up in 60 patients with congenital heart disease who underwent diagnostic (n=28) or therapeutic (n=32) cardiac catheterization procedures. The dose-saving effect of using extra copper filtration in the x-ray beam was also investigated. For diagnostic cardiac catheterizations, a median effective dose of 4.6 mSv was found. Therapeutic procedures resulted in a higher median effective dose of 6.0 mSv because of the prolonged use of fluoroscopy. The overall effect of inserting extra copper filtration into the x-ray beam was a total effective dose reduction of 18% with no detrimental effect on image quality. An excellent correlation between the dose-area product and effective patient dose was found (r=0.95). Hence, dose-area product is suitable for online estimation of the effective dose with good accuracy. With all procedures included, the resulting median lifetime risk for stochastic effects was 0.08%. Conclusions—Because of the high radiation exposure, it is important to monitor patient dose by dose-area product instrumentation and to use additional beam filtration to keep the effective dose as low as possible in view of the sensitivity of the pediatric patients.