Miracle-dz/UniFAR-FTUs huggingface dataset:
Interferon and ribavirin treatment in patients with hepatitis C-associated renal disease and renal insufficiency.

BACKGROUND Hepatitis C virus (HCV) infection is associated with renal manifestations, such as membranoproliferative glomerulonephritis (MPGN) with or without cryoglobulinaemia, membranous glomerulonephritis (MGN) and focal segmental glomerulosclerosis (FSGS). Standard treatment for HCV is interferon and ribavirin, but in renal insufficiency ribavirin has been contraindicated due to fear of side effects. METHODS Seven patients, two with cryoglobulinaemia, vasculitic manifestations and glomerulonephritis (GN), four with MPGN and one with FSGS were treated with a combination of interferon and ribavirin. Two patients were given pegylated interferon and ribavirin. All patients had at presentation renal insufficiency, with a glomerular filtration rate (GFR) between 10 and 65 ml/min. One patient had HCV genotype 1, the remainder 2 and 3. Duration of therapy was according to genotype (6-12 months). Ribavirin in plasma was monitored by high-performance liquid chromatography (HPLC) to avoid over-dosing, aiming at a target concentration of 10-15 micromol/l. The main side effect of ribavirin, haemolytic anaemia, was monitored closely with haemoglobin controls. RESULTS Six of seven patients became HCV-RNA-PCR negative and four of seven have maintained both virological and renal remission. One of seven has maintained virological and partial renal remission. One patient did not tolerate interferon, but is in renal remission with low-dose ribavirin. One vasculitis patient responded with complete remission, but relapsed virologically and had a minor vasculitic flare after 9 months. Only one patient with vasculitis had low-dose immunosuppression in addition to anti-viral therapy. Average daily ribavirin dose was 200-800 mg. Ribavirin-induced anaemia was managed in five of seven patients with low-dose iron and erythropoietin between 4000 and 20 000 IU/week. CONCLUSIONS Interferon and ribavirin can with reasonable safety be used in HCV-related vasculitis and GN irrespective of renal function.
Nanomaterial and toxicity: what can proteomics tell us about the nanotoxicology?

Abstract 1. In the last few years, a substantial scientific work is focused to identify the potential toxicity of nanomaterials by studying the cellular pathways under in vitro and in vivo conditions. Owing to high surface area to volume ratio nanoparticles (NPs) can pass through cell membranes which might be responsible for creating adverse interactions in biological systems. Simultaneously, researchers are also interested to assess the fate of NP inside the living system, which may lead to altered protein expression as well as protein corona formation. 2. According to published reports, NP-mediated toxicity involves altered cellular system including cell morphology, cell differentiation, cell metabolism, cell mobility, cellular immunity, which is derived from the side effects of nanoformulation and leading to apoptosis and necrosis. These results indicate the existence of potential toxic effect of these particles to human health. 3. The advent of proteomics with sophisticated technical improvement coupled with advanced bioinformatics has led to identify altered proteins due to nanomaterial exposure that could provide a new avenue to biomarker discovery. 4. This review aims to provide the current status of safe production and use of nanomaterials.
An internal ribosomal entry mechanism promotes translation of murine leukemia virus gag polyprotein precursors

The genomic retroviral RNA is the messenger for the translation of the gag and pol genes encoding the precursors to the major structural proteins and enzymes, respectively, of the virion core. The long 5' untranslated region, the leader, is formed of independent well-structured domains involved in key steps of the viral life cycle such as the initiation of proviral DNA synthesis, genomic RNA dimerization and packaging, and the initiation of gag translation. These functional features and the presence of stable secondary structures between the cap and the gag initiation codon suggested that translation initiation of gag might proceed through a mechanism different from the canonical ribosome scanning process. Interestingly enough, murine leukemia viruses code also for a glycosylated gag precursor, named glyco-gag, initiated at a CUG codon upstream and in the same open reading frame as the AUGgag. We have investigated the translation initiation of gag and glyco-gag precursors of Friend murine leukemia virus (F-MLV) in the rabbit reticulocyte lysate system and in murine cells. Through site-directed mutagenesis of gag and glyco-gag initiation codons, we show that initiation of gag and glyco-gag synthesis does not utilize the classical ribosome scanning. When poliovirus protease 2A is coexpressed in murine cells, expression of MLV-lacZ RNA is not modified, indicating that translation initiation of MLV gag precursors is a cap-independent mechanism. In addition, the F-MLV leader was inserted between two genes in a dicistronic neo-MLV-lacZ mRNA, and its ability to promote expression was examined in vitro and in vivo. Results obtained demonstrate that an internal ribosome entry mechanism promotes translation of F-MLV gag precursors. This finding led us to construct a new dicistronic retroviral vector in which the F-MLV leader can promote both packaging of recombinant genomic RNA and expression of the 3' gene.
Is Bentall Procedure Still the Gold Standard for Acute Aortic Dissection with Aortic Root Involvement?

Abstract Introduction The “ideal” treatment of acute aortic dissection type A (AADA) with dissected and dilated root is controversial. We compared the outcome of classical Bentall procedure (biological and mechanical) with valve-sparing David procedure. Methods Between January 2002 and July 2011, 119 patients with AADA and aortic root involvement underwent surgery at our center. Thirty-one patients (group 1) received biological conduits, 41 (group 2) received mechanical conduits, and 47 (group 3) underwent David procedures. Results Cross-clamp, cardiopulmonary bypass, and circulatory arrest times were 151 ± 52, 232 ± 84, and 36 ± 30 minutes (group 1); 148 ± 44, 237 ± 91, and 45 ± 29 minutes (group 2); and 160 ± 46, 231 ± 63, and 35 ± 17 minutes (group 3), respectively. The 30-day mortality rates were 32.3% (group 1), 22% (group 2), and 12.8% (group 3). The 1-year rates for freedom from valve-related reoperation were 100% (group 1), 92.5% (group 2), and 95.2% (group 3) (p = 0.172). The 1-year survival rates were 61% (group 1), 61% (group 2), and 84.1% (group 3) (p = 0.008). Conclusion Even in AADA patients with root involvement, David procedure has acceptable results. David procedure (if possible) or a Bio-Bentall (for pathological valves) seems to be the optimal technique.
The Bandim TBscore – reliability, further development, and evaluation of potential uses

Background The tuberculosis (TB) case detection rate has stagnated at 60% due to disorganized case finding and insensitivity of sputum smear microscopy. Of the identified TB cases, 4% die while being treated, monitored with tools that insufficiently predict failure/mortality. Objective To explore the TBscore, a recently proposed clinical severity measure for pulmonary TB (PTB) patients, and to refine, validate, and investigate its place in case finding. Design The TBscore's inter-observer agreement was assessed and compared to the Karnofsky Performance Score (KPS) (paper I). The TBscore's variables underlying constructs were assessed, sorting out unrelated items, proposing a more easily assessable TBscoreII, which was validated internally and externally (paper II). Finally, TBscore and TBscoreII's place in PTB-screening was examined in paper III. Results The inter-observer variability when grading PTB patients into severity classes was moderate for both TBscore (κ W=0.52, 95% CI 0.46–0.56) and KPS (κ W=0.49, 95% CI 0.33–0.65). KPS was influenced by HIV status, whereas TBscore was unaffected by it. In paper II, proposed TBscoreII was validated internally, in Guinea-Bissau, and externally, in Ethiopia. In both settings, a failure to bring down the score by ≥25% from baseline to 2 months of treatment predicted subsequent failure (p=0.007). Finally, in paper III, TBscore and TBscoreII were assessed in health-care-seeking adults and found to be higher in PTB-diagnosed patients, 4.9 (95% CI 4.6–5.2) and 3.9 (95% CI 3.8–4.0), respectively, versus patients not diagnosed with PTB, 3.0 (95% CI 2.7–3.2) and 2.4 (95% CI 2.3–2.5), respectively. Had we referred only patients with cough >2 weeks to sputum smear, we would have missed 32.1% of the smear confirmed cases in our cohort. A TBscoreII>=2 missed 8.6%. Conclusions TBscore and TBscoreII are useful monitoring tools for PTB patients on treatment, as they could fill the void which currently exists in risk grading of patients. They may also have a role in PTB screening; however, this requires our findings to be repeated elsewhere.
Assessment of lumbar spine kinematics using dynamic MRI: a proposed mechanism of sagittal plane motion induced by manual posterior-to-anterior mobilization.

STUDY DESIGN Descriptive study. OBJECTIVE The purpose of this study was to describe the segmental motion of the lumbar spine during a posterior-to-anterior (PA) mobilization procedure using dynamic magnetic resonance imaging and to propose a mechanism of the lumbar spine's motion as a result of a PA force to a lumbar spinous process. BACKGROUND Studies reporting kinematic descriptions of PA mobilization are in agreement that motion takes place at all lumbar vertebrae. However, these studies differ in the reported direction of motion. METHODS AND MEASURES Twenty asymptomatic subjects (mean age +/- SD, 31.1 +/- 7.0 years) participated in this study. For each subject, a PA mobilization force was manually applied at each lumbar spinous process while sagittal plane magnetic resonance images were simultaneously obtained. Intervertebral motion was defined as the change in the intervertebral angle between the resting and end range vertebral positions imparted by the PA pressure. RESULTS PA force applied at 1 spinous process caused motion at the target vertebra and this motion was propagated caudally and cranially. Motion at the target segment was always into extension. CONCLUSIONS A PA force applied at a single lumbar spinous process caused motion of the entire lumbar region. The magnitude and direction of intervertebral motions varied with the segment at which the PA force was applied. We postulated that the intervertebral motion induced by a PA force on a spinous process could be in part explained by the morphology of the lumbar spine.
Efficient Security for BGP Route Announcements

The Border Gateway Protocol (BGP) determines how Internet traffic is routed throughout the entire world; malicious behavior by one or more BGP speakers could create serious security issues. Since the protocol depends on a speaker honestly reporting path information sent by previous speakers and involves a large number of independent speakers, the Secure BGP (S-BGP) approach uses public-key cryptography to ensure that a malicious speaker cannot fabricate this information. However, such public-key cryptography is expensive: S-BGP requires a digital signature operation on each announcement sent to each peer, and a linear (in the length of the path) number of verifications on each receipt. We use simulation of a 110 AS system derived from the Internet to evaluate the impact that the processing costs of cryptography have on BGP convergence time. We find that under heavy load the convergence time using ordinary S-BGP is nearly twice as large as under BGP. We examine the impact of highly aggressive caching and pre-computation optimizations for S-BGP, and find that convergence time is much closer to BGP. However, these optimizations may be unrealistic, and are certainly expensive of memory. We consequently use the structure of BGP processing to design optimizations that reduce cryptographic overhead by amortizing the cost of private-key signatures over many messages. We call this method Signature-Amortization (S-A). We find that S-A provides as good or better convergence times as the highly optimized S-BGP, but without the cost and complications of caching and pre-computation. It is possible therefore to minimize the impact route validation has on convergence, by being careful with signatures, rather than consumptive of memory.
Ferrocement—Material Behavior in Flexure

The results of the program are derived from 80 tests on specimens in the form of planks measuring 36 in. x 6 in. x 1 in. (914 mm x 152 mm x 25 mm) subjected to third point loading in flexure. Variables include the type, amount, strength, and orientation of the reinforcement and the strength and density of the mortar. Comparisons of the performance of systems reinforced with expanded metal, welded mesh, woven mesh, parallel strand wire, and standard bars show that there are major differences with respect to both absolute strength and degree of conformity to the predictions of ultimate load analysis. Moreover, the order of superiority of the different systems depends on whether the application involves uniaxial or biaxial bending. Generally, these differences are associated with factors not taken into account in the standard load analysis-the geometry, orientation, and interlayer spacing of the reinforcement.
Mitral annular calcification – An overview -

Calcification of the mitral annulus has long been considered as a benign condition. However, recent investigations have shed a light on this condition. According to the severity of the disease, the result may be regurgitation or rarely stenosis of the mitral valve. In addition to the local effects, mitral annular calcification is considered as a surrogate for systemic inflammation, widespread calcifications and cardiovascular risk factors. Treatment is considered when calcification is severe enough to cause hemodynamic effects or is suspected to be a source of systemic embolization. Different surgical techniques are employed according to the localization and severity of the calcium deposition, the function of the valves and overall patient condition.
REMAP-experiments with speech recognition

We present experimental and theoretical results using a framework for training and modeling continuous speech recognition systems based on the theoretically optimal maximum a posteriori (MAP) criterion. This is in contrast to most state-of-the-art systems which are trained according to a maximum likelihood (ML) criterion. Although the algorithm is quite general, we applied it to a particular form of hybrid system combining hidden Markov models (HMMs) and artificial neural networks (ANNs) in which the ANN targets and weights are iteratively reestimated to guarantee the increase of the posterior probability of the correct model, hence actually minimizing the error rate. More specifically, this training approach is applied to a transition-based model that uses local conditional transition probabilities (i.e. the posterior probability of the current state given the current acoustic vector and the previous state) to estimate the posterior probabilities of sentences. Experimental results on isolated and continuous speech recognition tasks show an increase in the estimates of posterior probabilities of the correct sentences after training, and significant decreases in error rates in comparison to a baseline system.
Detection of interleukin 1beta (IL-1beta), IL-6, and tumor necrosis factor-alpha in skin of patients with fibromyalgia.

OBJECTIVE To determine if abnormal collagen metabolism is correlated with neurogenic inflammation, a potential activator of collagen metabolism, in patients with fibromyalgia (FM). METHODS The presence of inflammatory cytokines, interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-a was investigated in skin tissues by using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fifty-three skin biopsies from female patients with FM (30-65 years of age) were examined and compared to skin biopsies of 10 age and sex matched healthy controls. Biopsies were obtained from the left deltoid region. Rheumatoid arthritis synovial fibroblasts and tissues were used as positive controls for the expression of cytokines. Total RNA isolated from the tissue samples were reverse transcribed (RT) by random hexamers as the primer for RT followed by PCR amplification using specific primers for IL-1beta, IL-6 or TNF-a. Expression of IL-1beta, and TNF-a protein was investigated in the skin by immunohistochemistry using specific antibodies (avidin-biotin method). RESULTS Positive signals (RT-PCR) were detected in skin tissues of 19/50 (38%) FM patients for IL-1beta, in 14/51 FM patients (27%) for IL-6, and in 17/53 patients (32%) for TNF-a. None of the cytokines could be detected in healthy control skin. Immunoreactivity for IL-1beta and TNF-a was demonstrated in certain skin tissues of our FM patients. CONCLUSION The detection of cytokines in FM skin indicates the presence of inflammatory foci (neurogenic inflammation) in the skin of certain patients (about 30% of FM patients), suggesting an inflammatory component in the induction of pain. This may explain the response to nonsteroidal antiinflammatory therapy in a subset of FM patients.
How Precarious Employment Affects Health and Safety at Work: The Case of Temporary Agency Workers

Precarious employment has been associated with adverse occupational health and safety (OHS) outcomes across a range of studies. Temporary agency workers are particularly vulnerable, with studies showing they experience a higher incidence of workplace injury, and a greater likelihood of more severe injuries than all other employment types. Explanations for agency workers’ higher risk of injury have, to date, been impeded by data limitations associated with researching temporary employment. This article seeks to begin filling this gap through analyzing the experience of agency workers based upon two data sources. The first is a unique qualitative and quantitative data set developed from investigated temporary agency and directly hired workers’ compensation files; the second is focus groups of agency workers conducted in the State of Victoria, Australia. Quinlan and Bohle’s (2004) Pressures, Disorganization and Regulatory Failure (PDR) model, developed to explain the greater OHS vulnerability of precarious workers, provides the framework for analyzing the data. After explaining the key concepts in the PDR Model, the article analyses the data to test for evidence of economic pressures, disorganization at the workplace, and regulatory failure impacting upon temporary agency workers’ health and safety. The analysis supports the relevance of the PDR model, and provides an understanding of additional and unique risk factors which contribute to agency workers’ higher risk of injury. Temporary agency workers experience economic pressures in common with other types of precarious workers. However, these appear more acute amongst agency workers. They also confront disorganization risks, extending to mismatched placements; lack of familiarity with host workplaces; and more complex fractured communication. These contribute to workplace risks and create barriers to improving their experience. Many of these outcomes are a result of, or contribute to regulatory failure. The analysis finds strong support for the explanatory value of the PDR model as a tool for understanding how precariousness contributes to temporary agency workers’ adverse health and safety outcomes. It also suggests the complexities of the triangular employment relationship create additional economic insecurities and disorganization problems beyond those experienced by other types of workers, which the regulatory environment has yet to address.
Meningococcal Outer Membrane Vesicle Composition-Dependent Activation of the Innate Immune Response

ABSTRACT Meningococcal outer membrane vesicles (OMVs) have been extensively investigated and successfully implemented as vaccines. They contain pathogen-associated molecular patterns, including lipopolysaccharide (LPS), capable of triggering innate immunity. However, Neisseria meningitidis contains an extremely potent hexa-acylated LPS, leading to adverse effects when its OMVs are applied as vaccines. To create safe OMV vaccines, detergent treatment is generally used to reduce the LPS content. While effective, this method also leads to loss of protective antigens such as lipoproteins. Alternatively, genetic modification of LPS can reduce its toxicity. In the present study, we have compared the effects of standard OMV isolation methods using detergent or EDTA with those of genetic modifications of LPS to yield a penta-acylated lipid A (lpxL1 and pagL) on the in vitro induction of innate immune responses. The use of detergent decreased both Toll-like receptor 4 (TLR4) and TLR2 activation by OMVs, while the LPS modifications reduced only TLR4 activation. Mutational removal of PorB or lipoprotein factor H binding protein (fHbp), two proteins known to trigger TLR2 signaling, had no effect, indicating that multiple TLR2 ligands are removed by detergent treatment. Detergent-treated OMVs and lpxL1 OMVs showed similar reductions of cytokine profiles in the human monocytic cell line MM6 and human dendritic cells (DCs). OMVs with the alternative penta-acylated LPS structure obtained after PagL-mediated deacylation showed reduced induction of proinflammatory cytokines interleukin-6 (IL-6) and IL-1β but not of IP-10, a typical TRIF-dependent chemokine. Taken together, these data show that lipid A modification can be used to obtain OMVs with reduced activation of innate immunity, similar to what is found after detergent treatment.
Improved Markov Chain MBER Detection for Steered Linear Dispersion Coded MIMO Systems

In this paper, we present an iterative Markov Chain Minimum Bit Error Rate (MC-MBER) detection aided steered Linear Dispersion Coded (LDC) structure, which amalgamates three different Multiple-Input Multiple-Output (MIMO) func- tions, namely Space-Time Coding (STC), Spatial Division Mul- tiplexing (SDM) and beamforming. Furthermore, the concept of using an ovel ap rioriLog Likelihood Ratio (LLR) threshold based technique is invoked for the sake of further reducing the com- putational complexity imposed. Both the EXtrinsic Information Transfer (EXIT) chart analysis and our BER performance results demonstrate that the achievable performance is substantially improved upon increasing the number of iterations I. At BER = 1×10 �4 , the required Eb/N0 value is about 2.4 dB apart from that of the maximum achievable rate in conjunction with I =1 0 in a rank-deficient system transmitting Q = 6 QPSK modulated streams in T = 2 symbol durations with the aid of N = 2 receive antennas and M = 2 transmit antenna arrays, where each of the arrays is composed of L = 2 antenna elements. It is demonstrated that the computational complexity of the ap riori LLR threshold assisted MC-MBER detector is reduced by 38% at BER = 1.4×10 �5 in comparison to that of the MC-MBER detector dispensing with the thresholding technique.
Localization-induced Griffiths phase of disordered Anderson lattices.

We demonstrate that local density of states fluctuations in disordered Anderson lattice models universally lead to the emergence of non-Fermi liquid (NFL) behavior. The NFL regime appears at moderate disorder ( W = Wc) and is characterized by power-law anomalies, e.g., C/T approximately 1/T((1-alpha)), where the exponent alpha varies continuously with disorder, as in other Griffiths phases. This Griffiths phase is not associated with the proximity to any magnetic ordering, but reflects the approach to a disorder-driven metal-insulator transition (MIT). Remarkably, the MIT takes place only at much larger disorder W(MIT) approximately 12Wc, resulting in an extraordinarily robust NFL metallic phase.