Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Nitrate and Acetylene on nirS, cnorB, and nosZ Expression and Denitrification Activity in Pseudomonas mandelii

ABSTRACT Nitrate acts as an electron acceptor in the denitrification process. The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS, cnorB, and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured by using the acetylene blockage method and gas chromatography. The effect of acetylene on gene expression was assessed by comparing denitrification gene expression in P. mandelii culture grown in the presence or absence of acetylene. The higher the amount of NO3− present, the greater the induction and the longer the denitrification genes remained expressed. nirS gene expression reached a maximum at 2, 4, 4, and 6 h in cultures grown in the presence of 0, 10, 100, and 1,000 mg of KNO3/liter, respectively, while induction of nirS gene ranged from 12- to 225-fold compared to time zero. cnorB gene expression also followed a similar trend. nosZ gene expression did not respond to NO3− treatment under the conditions tested. Acetylene decreased nosZ gene expression but did not affect nirS or cnorB gene expression. These results showed that nirS and cnorB responded to nitrate concentrations; however, significant denitrification activity was only observed in culture with 1,000 mg of KNO3/liter, indicating that there was no relationship between gene expression and denitrification activity under the conditions tested.
Conservative Ideology and Ambivalent Sexism

To assess the relationship between different facets of conservative ideology and ambivalent sexism, 246 residents of two towns in southern Michigan completed a social dominance orientation scale (SDO), a right-wing authoritarianism scale (RWA), a Protestant work ethic scale (PWE), and the Glick and Fiske (1996) Ambivalent Sexism Inventory via a mail survey. Zero-order correlations revealed that SDO, RWA, and PWE were each related to both components of ambivalent sexism (hostile and benevolent sexism). Hierarchical regressions revealed that SDO and PWE most strongly predicted hostile sexism, whereas RWA most strongly predicted benevolent sexism. Results are discussed with respect to different facets of conservative ideology and why SDO, RWA, and PWE each tend to be associated with prejudice toward different groups.
Intracellular accumulation of azithromycin by cultured human fibroblasts

Azithromycin was shown to achieve high concentrations in human skin fibroblasts. Intracellular penetration occurred rapidly (10 micrograms/mg of cellular protein after 3 h) and then increased progressively over a 3-day period; azithromycin accumulated up to 21 times more than erythromycin (61.1 versus 2.9 micrograms/mg of protein). Uptake was dependent on the extracellular concentration, was inhibited at 4 degrees C, did not occur in nonviable cells, and was reduced by a low pH. Intracellular accumulation was not affected by the metabolic inhibitor 2,4-dinitrophenol or sodium fluoride or by the nucleoside transport inhibitor 2-chloradenosine. Once concentrated in cells, azithromycin remained intracellular and was released slowly in the absence of extracellular drug, compared with erythromycin (17 versus 78% released after 1 h). After 48 h of incubation in drug-free medium, 27% of the initial amount of azithromycin remained cell associated. The release of azithromycin was not affected by various monokines reported to stimulate fibroblasts (interleukin-1 or tumor necrosis factor) or by exposure to bacteria. Incubation of azithromycin-loaded fibroblasts with human polymorphonuclear leukocytes resulted in a higher intracellular accumulation of azithromycin in polymorphonuclear leukocytes than in cells incubated with free nonintracellular azithromycin for the same time (8.3 versus 2.2 micrograms/ml after 2 h), suggesting a more efficient or rapid uptake through cell-to-cell interaction. The widespread distribution of fibroblasts in tissues suggests a potential for these cells, and possibly other lysosome-containing tissue cells, to serve as a reservoir for azithromycin, slowly releasing it for activity against extracellular organisms at sites of infection and passing it to phagocytes for activity against intracellular pathogens and potential transport to sites of infection.
Estimation of physiological body parameters from smart garment data

A wearable smart garment containing embedded sensors produces live data stream like electrocardiograph, respiratory rate, tidal volume and body temperature. Additional vital body parameters are necessary to assess the physiological state of a person especially for athletes, firefighters, combat personnel etc. This work shows the development of a graphical user interface software application for estimation of parameters like metabolic rate, heart rate, heat stress index, core body temperature, sweat rate and heat strain following standard physiological equations. The smart garment and the software application can be used for remotely monitoring the health status of a person and taking necessary actions when required.
1H MR spectroscopy in common dementias

Objective: To determine the 1H MR spectroscopic (MRS) findings and intergroup differences among common dementias: Alzheimer disease (AD), vascular dementia (VaD), dementia with Lewy bodies (DLB), and frontotemporal lobar degeneration (FTLD). Methods: The authors consecutively recruited 206 normal elderly subjects and 121 patients with AD, 41 with FTLD, 20 with DLB, and 8 with VaD. The 1H MRS metabolite ratio changes in common dementias were evaluated with respect to normal and also differences among the common dementias. Results: N-Acetylaspartate (NAA)/creatine (Cr) was lower than normal in patients with AD, FTLD, and VaD. Myo-inositol (mI)/Cr was higher than normal in patients with AD and FTLD. Choline (Cho)/Cr was higher than normal in patients with AD, FTLD, and DLB. There were no metabolite differences between patients with AD and FTLD or between patients with DLB and VaD. NAA/Cr was lower in patients with AD and FTLD than DLB. MI/Cr was higher in patients with AD and FTLD than VaD. MI/Cr was also higher in patients with FTLD than DLB. Conclusions: NAA/Cr levels are decreased in dementias that are characterized by neuron loss, such as AD, FTLD, and VaD. MI/Cr levels are elevated in dementias that are pathologically characterized by gliosis, such as AD and FTLD. Cho/Cr levels are elevated in dementias that are characterized by a profound cholinergic deficit, such as AD and DLB.
Probing scientists' beliefs: how open‐minded are modern scientists?

Just how open‐minded are modern scientists? In this paper we examine this question for the science faculty from New Zealand and UK universities. The Exeter questionnaire used by Preece and Baxter (2000) to examine superstitious beliefs of high school students and preservice science teachers was used as a basis for a series of in‐depth interviews of scientists across a variety of disciplines. The interviews sought to understand the basis on which scientists form beliefs and how they judge evidence for various propositions, including those from the Exeter questionnaire and other contentious beliefs introduced during discourse. The scientists are dismissive of traditional superstitions like bad luck associated with black cats and inauspicious numbers such as 13, seeing such beliefs as socially grounded. There is a strong socio‐cultural aspect to other beliefs and personal experiences, and strongly held personal beliefs are influential, resulting in the scientists keeping an open mind about contentious beliefs like alien life and the existence of ghosts. Testimony of others including media reports are deemed unreliable unless provided by credible witnesses such as ‘educated people’ or ‘experts’, or if they coincide with the scientists' personal beliefs. These scientists see a need for potential theoretical explanations for beliefs and are generally dismissive of empirical evidence without underlying explanations.
Leishmania UDP-sugar Pyrophosphorylase

The Leishmania parasite glycocalyx is rich in galactose-containing glycoconjugates that are synthesized by specific glycosyltransferases that use UDP-galactose as a glycosyl donor. UDP-galactose biosynthesis is thought to be predominantly a de novo process involving epimerization of the abundant nucleotide sugar UDP-glucose by the UDP-glucose 4-epimerase, although galactose salvage from the environment has been demonstrated for Leishmania major. Here, we present the characterization of an L. major UDP-sugar pyrophosphorylase able to reversibly activate galactose 1-phosphate into UDP-galactose thus proving the existence of the Isselbacher salvage pathway in this parasite. The ordered bisubstrate mechanism and high affinity of the enzyme for UTP seem to favor the synthesis of nucleotide sugar rather than their pyrophosphorolysis. Although L. major UDP-sugar pyrophosphorylase preferentially activates galactose 1-phosphate and glucose 1-phosphate, the enzyme is able to act on a variety of hexose 1-phosphates as well as pentose 1-phosphates but not hexosamine 1-phosphates and hence presents a broad in vitro specificity. The newly identified enzyme exhibits a low but significant homology with UDP-glucose pyrophosphorylases and conserved in particular is the pyrophosphorylase consensus sequence and residues involved in nucleotide and phosphate binding. Saturation transfer difference NMR spectroscopy experiments confirm the importance of these moieties for substrate binding. The described leishmanial enzyme is closely related to plant UDP-sugar pyrophosphorylases and presents a similar substrate specificity suggesting their common origin.
Study of mechanical characteristics of the knee extensor and flexor musculature of volleyball players

Abstract The aim of the present study was to analyse differences in muscle response and mechanical characteristics of the vastus medialis, rectus femoris, vastus lateralis, and biceps femoris in elite volleyball players of both sexes using tensiomyography. To this end, 47 players of nine nationalities playing for teams in the men's and women's Spanish Superleagues were assessed. The sample comprised 22 women (age 24.6±4.3 years; weight 72.14±10.06 kg; height 178.40±8.50 cm) and 25 men (age 25.0±4.3 years; weight 88.76±9.07 kg; height 194.71±7.84 cm). Tensiomyography was used to assess muscular response and muscular mechanical characteristics. For this purpose, the following variables were analysed: maximum radial displacement of muscle belly and normalized response speed. The findings show, both in men and women, a higher normalized response speed score in the vastus lateralis and vastus medialis compared with the rectus femoris and biceps femoris. A marked lateral symmetry of maximum radial displacement of the muscle belly was also observed in the musculature of the lower limbs, with no statistically significant differences being detected in either men or women. There were, however, clear differences in terms of normalized response speed between male and female volleyball players: women displayed a more pronounced difference in the normalized response speed of the musculature responsible for extension (vastus medialis, rectus femoris, and vastus lateralis) and flexion (biceps femoris) of the knee joint than men. Moreover, tensiomyography proved to be a highly sensitive tool for detecting such changes.
Transfer from Word Training to Reading in Context: Gains in Reading Fluency and Comprehension

This article reports two studies that examined the relationship between word identification speed and story reading fluency, as indicated by speed and accuracy as well as comprehension. Poor readers in grade 4 were trained to read a set of single words and were then asked to repeatedly read stories that contained the trained words or stories with words not included in the training set. Benefits to text reading from single-word practice were observed, even for children who were particularly slow namers. The results are related to theoretical links between fluency and comprehension and to theories of developmental deficits.
Homeless Women, Special Possessions, and the Meaning of “Home”: An Ethnographic Case Study

This article investigates homelessness among adult women, an important and growing subpopulation among the homeless. To examine their situation within a consumer-behavior context, an ethnographic case study of a shelter for homeless women run by an order of Roman Catholic sisters was performed. The study focused on how these women became homeless, the effects of early life experiences on their homelessness, available emotional and financial support, possessions that were lost, maintained, or became available during their homeless period(s), their perspectives on their lives at the shelter and its ability to act as a "home," and their fantasies about home life. Public policy implications and contributions of these findings to the developing literature in consumer behavior regarding the meaning of possessions are discussed. Copyright 1991 by the University of Chicago.
Stable, Precise, and Reproducible Patterning of Bicoid and Hunchback Molecules in the Early Drosophila Embryo

Precise patterning of morphogen molecules and their accurate reading out are of key importance in embryonic development. Recent experiments have visualized distributions of proteins in developing embryos and shown that the gradient of concentration of Bicoid morphogen in Drosophila embryos is established rapidly after fertilization and remains stable through syncytial mitoses. This stable Bicoid gradient is read out in a precise way to distribute Hunchback with small fluctuations in each embryo and in a reproducible way, with small embryo-to-embryo fluctuation. The mechanisms of such stable, precise, and reproducible patterning through noisy cellular processes, however, still remain mysterious. To address these issues, here we develop the one- and three-dimensional stochastic models of the early Drosophila embryo. The simulated results show that the fluctuation in expression of the hunchback gene is dominated by the random arrival of Bicoid at the hunchback enhancer. Slow diffusion of Hunchback protein, however, averages out this intense fluctuation, leading to the precise patterning of distribution of Hunchback without loss of sharpness of the boundary of its distribution. The coordinated rates of diffusion and transport of input Bicoid and output Hunchback play decisive roles in suppressing fluctuations arising from the dynamical structure change in embryos and those arising from the random diffusion of molecules, and give rise to the stable, precise, and reproducible patterning of Bicoid and Hunchback distributions.
The Intraocular Pressure under Deep versus Moderate Neuromuscular Blockade during Low-Pressure Robot Assisted Laparoscopic Radical Prostatectomy in a Randomized Trial

Background This study aimed to determine whether continuous deep neuromuscular blockade (NMB) improves the surgical conditions and facilitates robotic-assisted laparoscopic radical prostatectomy (RALRP) under low intra-abdominal pressure (IAP) to attenuate the increase in intraocular pressure (IOP) during CO2 pneumoperitoneum in the steep Trendelenburg (ST) position. Methods Sixty-seven patients undergoing RALRP were randomly assigned to a moderate NMB group (Group M), including patients who received atracurium infusion until the end of the ST position, maintaining a train of four count of 1–2; and the deep NMB group (Group D), including patients who received rocuronium infusion, maintaining a post-tetanic count of 1–2. IOP was measured in all patients at nine separate time points. All RALRPs were performed by one surgeon, who rated the overall and worst surgical conditions at the end of the ST position. Results The highest IOP value was observed at T4 (60 min after the ST position) in both Group M (23.3 ± 2.7 mmHg) and Group D (19.8 ± 2.1 mmHg). RALRP was accomplished at an IAP of 8 mmHg in 88% Group D patients and 25% Group M patients. The overall surgical condition grade was 4.0 (3.0–5.0) in Group D and 3.0 (2.0–5.0) in Group M (P < 0.001). Conclusion The current study demonstrated that continuous deep NMB may improve surgical conditions and facilitate RALRP at a low IAP, resulting in significant attenuation of the increase on IOP. Moreover, low-pressure pneumoperitoneum, facilitated by deep NMB still provided acceptable surgical conditions. Trial Registration ClinicalTrials.gov NCT02109133
Introduction: Knowledge Management in Professional Service Firms

Knowledge represents a highly valuable organizational resource. Organizations should pay careful attention to how they manage knowledge. Professional service firms (PSFs) have much to teach other organizations about knowledge management. In the course of the 1990s these assertions progressed from being novel ideas to becoming well-worn cliches. Learned academic journals and airport bookshelves are now crowded with works which seek to define the nature of knowledge and identify how it can be managed. Librarians and IT support staff, traditionally marginalized within organizations, have found themselves re-branded as ‘key information resources’ and ‘chief knowledge officers’. Accountants and consulting firms, typically relegated to a professional services ghetto within the management literature, have been thrust into the foreground as exemplars of best practice in the field of knowledge management. Such is the interest in the topic of knowledge management that a backlash is almost certainly imminent. Bertels and Savage (1998) have suggested that ‘after three years of intense fluff, the lemmings will be on to their next topic, without ever having really mined the subject of knowledge’ (p. 7). This special issue of Human Relations seeks to make a lasting contribution to our understanding of the role of knowledge within organizations. It brings together researchers from a variety of disciplinary and theoretical perspectives who are united by their common interest in the management of knowledge within PSFs. Why has knowledge management become such a ‘hot’ topic in recent years? Some might argue that it is the result of effective marketing by consulting firms (see Suddaby and Greenwood in this issue). Others suggest that academics are inherently attracted to ideationalist theories because, as
Changes in bone sclerostin levels in mice after ovariectomy vary independently of changes in serum sclerostin levels

We examined the effects that ovariectomy had on sclerostin mRNA and protein levels in the bones of 8‐week‐old mice that were either sham‐operated (SHAM) or ovariectomized (OVX) and then euthanized 3 or 6 weeks later. In this model, bone loss occurred between 3 and 5 weeks postsurgery. In calvaria, ovariectomy significantly decreased sclerostin mRNA levels at 6 weeks postsurgery (by 52%) but had no significant effect at 3 weeks. In contrast, sclerostin mRNA levels were significantly lower in OVX femurs at 3 weeks postsurgery (by 53%) but equal to that of SHAM at 6 weeks. The effects of ovariectomy on sclerostin were not a global response of osteocytes because they were not mimicked by changes in the mRNA levels for two other relatively osteocyte‐specific genes: DMP‐1 and FGF‐23. Sclerostin protein decreased by 83% and 60%, at 3 and 6 weeks postsurgery in calvaria, respectively, and by 38% in lumbar vertebrae at 6 weeks. We also detected decreases in sclerostin by immunohistochemistry in cortical osteocytes of the humerus at 3 weeks postsurgery. However, there were no significant effects of ovariectomy on sclerostin protein in femurs or on serum sclerostin at 3 and 6 weeks postsurgery. These results demonstrate that ovariectomy has variable effects on sclerostin mRNA and protein in mice, which are dependent on the bones examined and the time after surgery. Given the discrepancy between the effects of ovariectomy on serum sclerostin levels and sclerostin mRNA and protein levels in various bones, these results argue that, at least in mice, serum sclerostin levels may not accurately reflect changes in the local production of sclerostin in bones. Additional studies are needed to evaluate whether this is also the case in humans. © 2013 American Society for Bone and Mineral Research.
Modernising Social Work and the Ethics of Care

Recent attempts to 'modernise' social work have emphasised the importance of collaboration, partnership, and participation with individual users of services and the wider community. However, technical-rational aspects of managerialism have proved dominant. Managerialist approaches to social service administration and delivery threaten important dimensions of social work; specifically its caring and democratic-transformative dimensions. However, social work theorists have only recently begun to re-engage with ideas of care. We argue that closer attention to feminist debates about the ethics of care can make a significant contribution to not only rehabilitating the ideal of care for social work but also to moving forward the modernisation agenda itself. We develop a feminist critique of managerialism, and argue that the discourse of the ethics of care offers useful ways of framing arguments to counter some damaging impacts of managerial reforms.
Cooperation model of a multiagent parallel file system for clusters

MAPFS is a parallel file system integrated with a multiagent system responsible for the information retrieval. One of the fields where the agents can be very useful is precisely in the development of information recovery systems. The usage of a multiagent system implies coordination among the agents that belong to such system. The main goal of the agent cooperation is the interaction among them for achieving a common objective in a distributed system. Thus, a communication framework must be provided. This paper shows the MAPFS cooperation model and its communication framework, emphasizing its relation with the whole system.
Assessing Operations Management from a Strategic Perspective

Manufacturing strategy research, together with service operations and strategy research, form the background for an examination of operations management. Although much of the assessment and critique is directed at operations strategy, traditional operations management issues such as technology, productivity, and quality also come under scrutiny as to their role in strategy. This review identifies three common themes and seven missing themes in the literature, contrasting the "'content" variables so frequently investigated within operations management-quality, cost, delivery, and flexibility-against strategic "process" variables. Conclusions include the partial indictment of operations management literature through the missing themes, an indictment that can be shored-up through increased empirical research in operations strategy that focuses on content, process, and performance.
Impaired Glucose Tolerance, Insulin Resistance and Cardiovascular Disease

Non‐insulin‐dependent diabetes (NIDDM) is associated with a higher risk of cardiovascular disease (CVD) than in the non‐diabetic population. The incidence of CVD is not, however, related to the severity or duration of disease in most studies, suggesting that events in the prediabetic stage can trigger CVD. Several factors have been linked to CVD, including hyperglycaemia, insulin resistance and hyperinsulinaemia. Impaired glucose tolerance (IGT), as defined by the oral glucose tolerance test, is a strong predictor of NIDDM. In addition, IGT and raised glycated haemoglobin levels are possible risk factors for CVD. Hyperinsulinaemia has been associated with increased cardiovascular risk, but this has yet to be confirmed in women. There is also evidence to link insulin resistance with increased cardiovascular risk, although the precise nature of this relationship is unclear. There are many risk factors associated with CVD that are probably working in concert in NIDDM. This makes assessment of risk posed by individual factors, such as hyperglycaemia, hyperinsulinaemia and insulin sensitivity, difficult. © 1997 John Wiley & Sons, Ltd.