Miracle-dz/UniFAR-FTUs huggingface dataset:
Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe

In conventional optical tweezers system a high numerical aperture (NA) objective is employed both to image and to generate a gradient force toward the focus on sample particles, so the system is complex and expensive especially for the multi-optical-tweezers system. We built a novel simple optical trapping system based on a lensed optical fiber probe. This new method offers several other advantages over the conventional optical tweezers. The trapping system we built includes a laser coupling unit, a multi-dimensional probe manipulating unit, a sample nano-positioning unit, and a microscopy imaging unit. Based on the system, a yeast cell is trapped and manipulated on the chamber bottom by the lensed fiber probe, and the optical trapping forces acting on the yeast cell as a function of the offset are measured and discussed in different directions by the static method and the dynamic one respectively with various powers. The results by the two measurement methods coincide with each other, and the detail experimental procedure and the data processing of the two methods are introduced in this paper.
Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila

The wing of Drosophila melanogaster is covered by an array of distally pointing hairs. A hair begins as a single membrane outgrowth from each wing epithelial cell, and its distal orientation is determined by the restriction of outgrowth to a single distal site on the cell circumference (Wong, L., and P. Adler. 1993. J. Cell Biol. 123:209- 211.). We have examined the roles of Cdc42 and Rac1 in the formation of wing hairs. We find that Cdc42 is required for localized actin polymerization in the extending hair. Interfering with Cdc42 activity by expression of a dominant negative protein abolishes both localized actin polymerization and hair outgrowth. In contrast, Rac1 is important for restricting the site at which hairs grow out. Cells expressing the dominant negative Rac1N17 fail to restrict outgrowth to a single site and give rise to multiple wing hairs. This polarity defect is associated with disturbances in the organization of junctional actin and also with disruption of an intricate microtubule network that is intimately associated with the junctional region. We also find that apical junctions and microtubules are involved in structural aspects of hair outgrowth. During hair formation, the apical microtubules that point distally elongate and fill the emerging wing hair. As the hair elongates, junctional proteins are reorganized on the proximal and distal edges of each cell.
Translocation of hormone-sensitive lipase and perilipin upon lipolytic stimulation during the lactation cycle of the rat.

The removal of the litter from lactating rats results in a decrease in the lipolytic response to catecholamines in maternal adipocytes; this effect can be prevented by concomitant treatment of the rats with growth hormone. The decrease in response to catecholamines following litter removal was not due to a change in the amount of either hormone-sensitive lipase (HSL) or perilipin per adipocyte or in the proportion of either of these proteins associated with the fat droplet. Incubation in vitro with isoproterenol did not cause any apparent net translocation of HSL to the fat droplet in adipocytes from the mature female rats in any state used in this study, but isoproterenol did cause a movement of perlipin away from the fat droplet. This translocation of perilipin was not altered by litter removal. Thus, the decrease in response to catecholamines found on litter removal from lactating rats appears to be due to a diminished ability to activate HSL associated with fat droplet.
CAFE: a configurable packet forwarding engine for data center networks

Recently, Data Center Networking (DCN) has attracted many research attentions and innovative DCN designs have been proposed [1, 2]. All these designs need specialized packet forwarding engines due to their special routing algorithms, which are either based on commonly used packet headers or self-defined ones. Although programmable forwarding devices are available, it is difficult to use them to prototype these DCN designs, especially when self-defined headers are introduced. In this paper, we present a hardware based Configurable pAcket Forwarding Engine (CAFE) to facilitate the prototyping process. Through simple APIs, CAFE can be easily configured to forward self-defined packets, modify, insert, and delete arbitrary packet header fields without re-designing the hardware. We have implemented CAFE using NetFPGA. Evaluation demonstrates that CAFE can be easily configured and it can forward packets at line-rate.
Endocytosis from coated pits of Shiga toxin: a glycolipid-binding protein from Shigella dysenteriae 1

Evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from Shigella dysenteriae 1, Shiga toxin, which acts by removal of a single adenine residue in 28-S ribosomal RNA. Inhibition of endocytosis by ATP depletion of the cells prevented toxin uptake. Exposure of HeLa S3 and Vero cells to toxin at low extracellular pH, where translocation to the cytosol, but not endocytosis is inhibited, allowed the toxin to accumulate in a compartment where it was protected against antibodies to the toxin. Upon transfer of the cells to normal medium endocytosed toxin entered the cytosol. Electron microscopical studies of cells exposed at 0 degrees C to a toxin-horseradish peroxidase (HRP) conjugate, or to unconjugated toxin followed by horse antitoxin antibodies and then protein G-gold, revealed that the Shiga toxin binding sites were randomly distributed on the cell surface, without any preference to, for example, coated pits. In contrast, when cells were exposed to toxin at 37 degrees C, the binding sites were preferentially localized in coated pits. The Shiga-HRP conjugate was also seen in endosomes, lysosomes, and in the Golgi region. Endocytosis by the coated pit/coated vesicle pathway was selectively inhibited by acidification of the cytosol. Under these conditions, both the uptake of toxin-HRP conjugates and intoxication of the cells were inhibited. Evidence from the literature as well as our own results suggest that Shiga toxin binding sites are glycolipids. Thus, Shiga toxin appears to be the first example of a lipid-binding ligand that is endocytosed from coated pits.
Magnetostriction and the Influence of Higher Harmonics in the Magnetic Field

Knowing the effect of the higher harmonics of the magnetic field on the magnetostrictive behavior of electrical steel is highly beneficial for the noise aspects. In this work, the effect of a third harmonic with a variety of amplitudes and phase delays is extensively studied. Magnetostriction measurements are performed on samples of grain-oriented and nonoriented electrical steel under sinusoidal excitations with a third harmonic component and are compared with those of a purely sinusoidal excitation. The frequency spectrum of the measured data, where the second and the fourth harmonic are the most significant ones are then calculated. The measured data show that only knowing the amplitude of the third harmonic is not sufficient to estimate the variations of the magnetostriction. In fact the phase delay of such third harmonic can significantly alter the results. The measurements are carried out on two grain-oriented and two nonoriented samples under a magnetization with a fundamental of 1.3 T and 50 Hz, from which the following conclusions are drawn. Increasing the phase delay of a 8% third harmonic ratio from 0° to 180° delay, causes a variation up to 55% of the 100 Hz component for the two nonoriented materials. In the case of grain-oriented materials, up to 50% change is obtained. The measured data of the 200 Hz components show an increase, as well. However, since their values are far lower than the 100 Hz component, their effect is considerably smaller on the magnetostrictive behavior. These results are highly important for the noise of electrical machines and transformers, due to the magnetostriction phenomenon.
Mechanotransduction – a field pulling together?

Mechanical stresses are ever present in the cellular environment, whether through external forces that are applied to tissues or endogenous forces that are generated within the active cytoskeleton. Despite the wide array of studies demonstrating that such forces affect cellular signaling and function, it remains unclear whether mechanotransduction in different contexts shares common mechanisms. Here, I discuss possible mechanisms by which applied forces, cell-generated forces and changes in substrate mechanics could exert changes in cell function through common mechanotransduction machinery. I draw from examples that are primarily focused on the role of adhesions in transducing mechanical forces. Based on this discussion, emerging themes arise that connect these different areas of inquiry and suggest multiple avenues for future studies.
Fasting Cycles Retard Growth of Tumors and Sensitize a Range of Cancer Cell Types to Chemotherapy

Short-term starvation increases the effectiveness of chemotherapy against a wide range of tumor cell types. Fasting: Good for You, Bad for Tumors Many promising cancer drugs being developed will require years to become approved by regulatory bodies and, in most cases, will only be effective for a fraction of patients with specific types of cancer. It is therefore important to develop broader, complementary strategies that can be translated rapidly into effective therapies. Two to 4 days of fasting before chemotherapy treatment is safe and protect animals, and possibly humans, against the side effects of chemotherapy. Here, cycles of fasting for 2 days in the absence of other treatments are shown to delay the progression of several tumor types in mice and, in some cases, to be as effective as toxic chemotherapy drugs. However, the combination of fasting and chemotherapy was much more effective than either alone and delayed the progression of a variety of tumors, including breast cancer and glioma, reduced the number of organs affected by melanoma metastases, and promoted long-term cancer-free survival in up to 40% of mice with neuroblastomas. In mice injected with human breast and ovarian cancer cells, fasting cycles promoted survival extension by protecting the mice from chemotherapy while causing a strong inhibition of tumor progression. Experiments in simple organisms, human cells, and mice indicated that these effects of fasting were caused by changes inside and outside cells that increased the death of tumor but not normal cells, a process termed differential stress sensitization. Although clinical trials testing the effect of fasting in cancer treatment are still in the early stages, they suggest that fasting cycles may boost the efficacy of chemotherapeutic agents and could be as effective as chemotherapy drugs in the killing of specific tumor cells. Short-term starvation (or fasting) protects normal cells, mice, and potentially humans from the harmful side effects of a variety of chemotherapy drugs. Here, we show that treatment with starvation conditions sensitized yeast cells (Saccharomyces cerevisiae) expressing the oncogene-like RAS2val19 to oxidative stress and 15 of 17 mammalian cancer cell lines to chemotherapeutic agents. Cycles of starvation were as effective as chemotherapeutic agents in delaying progression of different tumors and increased the effectiveness of these drugs against melanoma, glioma, and breast cancer cells. In mouse models of neuroblastoma, fasting cycles plus chemotherapy drugs—but not either treatment alone—resulted in long-term cancer-free survival. In 4T1 breast cancer cells, short-term starvation resulted in increased phosphorylation of the stress-sensitizing Akt and S6 kinases, increased oxidative stress, caspase-3 cleavage, DNA damage, and apoptosis. These studies suggest that multiple cycles of fasting promote differential stress sensitization in a wide range of tumors and could potentially replace or augment the efficacy of certain chemotherapy drugs in the treatment of various cancers.
Absolute Blood Flow Measurement of early Stage Chicken Embryo Heart Using High Speed 4D Optical Coherence Tomography

The measurement of blood-plasma absolute velocity distributions with spatial and temporal resolution in vivo is important for research on early stage embryo heart development. We introduce a novel method to measure absolute blood flow velocity based on high speed spectral domain Optical Coherence Tomography (OCT) and applied it to measure velocities on the heart outflow tract (OFT) of a chicken embryo (stage HH18).
Hospitalization Within the First Year After Stroke: The Dijon Stroke Registry

Background and Purpose— This population-based study aimed to identify unplanned hospitalization within the first year after stroke to determine factors associated with it and consequences on survival. Methods— All first-ever acute strokes occurring in Dijon, France, from 2009 to 2011, were prospectively collected from a population-based registry. Demographics and clinical data, including stroke severity measured by the National Institutes of Health Stroke Scale and disability after stroke, were recorded. For each patient, the first unplanned hospitalization that occurred within 1 year after stroke was retrieved by linking data with the national French Hospital Discharge Database. Predictors of hospitalization and survival at 1 year were identified using logistic regression models. Results— Among the 613 patients recorded, 94 (15.3%) were excluded because of early death. Of the 519 remaining patients, 167 (32.2%) were hospitalized at 1 year. Subsequent hospitalization led to in-hospital death for 16 (9.6%) patients. In multivariable analyses, only a history of hypertension and atrial fibrillation were associated with hospitalization. In stratified analyses, the National Institutes of Health Stroke Scale score was associated with a higher risk of hospitalization (odds ratio, 1.13; 95% confidence interval, 1.03–1.22; P=0.006), whereas only a trend was noted for disability (odds ratio, 2.26; 95% confidence interval, 0.82–6.22; P=0.113) in patients who returned home after the index stroke. Hospitalization was negatively associated with being alive at 1 year (odds ratio, 0.36; 95% confidence interval, 0.19–0.66; P<0.01). Conclusions— Stroke survivors are at high risk of hospitalization after the episode, and subsequent admission is associated with poor survival, thus highlighting the need for follow-up interventions after discharge to prevent readmission.
Export-oriented deforestation in Mato Grosso: harbinger or exception for other tropical forests?

The Brazilian state of Mato Grosso was a global deforestation hotspot in the early 2000s. Deforested land is used predominantly to produce meat for distal consumption either through cattle ranching or soya bean for livestock feed. Deforestation declined dramatically in the latter part of the decade through a combination of market forces, policies, enforcement and improved monitoring. This study assesses how representative the national-level drivers underlying Mato Grosso's export-oriented deforestation are in other tropical forest countries based on agricultural exports, commercial agriculture and urbanization. We also assess how pervasive the governance and technical monitoring capacity that enabled Mato Grosso's decline in deforestation is in other countries. We find that between 41 and 54 per cent of 2000–2005 deforestation in tropical forest countries (other than Brazil) occurred in countries with drivers similar to Brazil. Very few countries had national-level governance and capacity similar to Brazil. Results suggest that the ecological, hydrological and social consequences of land-use change for export-oriented agriculture as discussed in this Theme Issue were applicable in about one-third of all tropical forest countries in 2000–2005. However, the feasibility of replicating Mato Grosso's success with controlling deforestation is more limited. Production landscapes to support distal consumption similar to Mato Grosso are likely to become more prevalent and are unlikely to follow a land-use transition model with increasing forest cover.
Audio forensic authentication based on MOCC between ENF and reference signals

This paper proposes a new audio authenticity detection algorithm based on the max offset for cross correlation (MOCC) between the extracted ENF (Electric Network Frequency) signal and the reference signal. We first extract the ENF signal from a query audio signal. And then we partition it into overlapping blocks for forgery detection. The MOCC between the extracted ENF and the reference signal is calculated block by block. We also introduce an enhancement scheme to improve the quality of the ENF signal before the calculation of the MOCC. Our proposed method can detect not only audio forgery but also the edited region and audio forgery type. The effectiveness of our method has been verified by experiments on digitally edited audio signals.
The Design and Analysis of Benchmark Experiments

The assessment of the performance of learners by means of benchmark experiments is an established exercise. In practice, benchmark studies are a tool to compare the performance of several competing algorithms for a certain learning problem. Cross-validation or resampling techniques are commonly used to derive point estimates of the performances which are compared to identify algorithms with good properties. For several benchmarking problems, test procedures taking the variability of those point estimates into account have been suggested. Most of the recently proposed inference procedures are based on special variance estimators for the cross-validated performance. We introduce a theoretical framework for inference problems in benchmark experiments and show that standard statistical test procedures can be used to test for differences in the performances. The theory is based on well-defined distributions of performance measures which can be compared with established tests. To demonstrate the usefulness in practice, the theoretical results are applied to regression and classification benchmark studies based on artificial and real world data.
Managing change in software development using a process improvement approach

Describes the DESIRE (DESigned Improvements in Requirements Evolution) project, which is investigating novel ways to better manage change during software development. Our work has identified five key process improvement practices: (1) adopting a change improvement framework; (2) establishing an organisation-wide change process; (3) classifying change; (4) change estimation; and (5) measuring change improvement. In this paper, we describe each practice in detail and propose a change maturity model that reflects an organisation's capability to manage change.
Cost-effectiveness of 10-Year Risk Thresholds for Initiation of Statin Therapy for Primary Prevention of Cardiovascular Disease.

IMPORTANCE The American College of Cardiology and the American Heart Association (ACC/AHA) cholesterol treatment guidelines have wide-scale implications for treating adults without history of atherosclerotic cardiovascular disease (ASCVD) with statins. OBJECTIVE To estimate the cost-effectiveness of various 10-year ASCVD risk thresholds that could be used in the ACC/AHA cholesterol treatment guidelines. DESIGN, SETTING, AND PARTICIPANTS Microsimulation model, including lifetime time horizon, US societal perspective, 3% discount rate for costs, and health outcomes. In the model, hypothetical individuals from a representative US population aged 40 to 75 years received statin treatment, experienced ASCVD events, and died from ASCVD-related or non-ASCVD-related causes based on ASCVD natural history and statin treatment parameters. Data sources for model parameters included National Health and Nutrition Examination Surveys, large clinical trials and meta-analyses for statin benefits and treatment, and other published sources. MAIN OUTCOMES AND MEASURES Estimated ASCVD events prevented and incremental costs per quality-adjusted life-year (QALY) gained. RESULTS In the base-case scenario, the current ASCVD threshold of 7.5% or higher, which was estimated to be associated with 48% of adults treated with statins, had an incremental cost-effectiveness ratio (ICER) of $37,000/QALY compared with a 10% or higher threshold. More lenient ASCVD thresholds of 4.0% or higher (61% of adults treated) and 3.0% or higher (67% of adults treated) had ICERs of $81,000/QALY and $140,000/QALY, respectively. Shifting from a 7.5% or higher ASCVD risk threshold to a 3.0% or higher ASCVD risk threshold was estimated to be associated with an additional 161,560 cardiovascular disease events averted. Cost-effectiveness results were sensitive to changes in the disutility associated with taking a pill daily, statin price, and the risk of statin-induced diabetes. In probabilistic sensitivity analysis, there was a higher than 93% chance that the optimal ASCVD threshold was 5.0% or lower using a cost-effectiveness threshold of $100,000/QALY. CONCLUSIONS AND RELEVANCE In this microsimulation model of US adults aged 45 to 75 years [corrected], the current 10-year ASCVD risk threshold (≥7.5% risk threshold) used in the ACC/AHA cholesterol treatment guidelines has an acceptable cost-effectiveness profile (ICER, $37,000/QALY), but more lenient ASCVD thresholds would be optimal using cost-effectiveness thresholds of $100,000/QALY (≥4.0% risk threshold) or $150,000/QALY (≥3.0% risk threshold). The optimal ASCVD threshold was sensitive to patient preferences for taking a pill daily, changes to statin price, and the risk of statin-induced diabetes.
Effect of rod-like grain on properties and toughening mechanism of 3Y-TZP/Al2O3 ceramics

在原处像杆的 Al2O3 谷物被在 30 MPa， 1 550 ° C 和 1 h 的 sintering 条件下面增加 CAS 多相的添加剂准备。 Al2O3 陶艺的 sintering 行为，微观结构，韧化的机制和存取被宣传的无所不能的电子机械测试机器，等等，和裂缝圆柱的 crystal/3Y-TZP 建模的 SEM，版本和 WDW 调查合成韧化 Al2O3 陶艺被建立。 结果证明合成添加剂推动 Al2O3 谷物的各向异性的生长，它加强在 3Y-TZP/Al2O3 韧化 3Y-TZP 的效果合成陶艺。 而且，试验性的材料密度几乎理论密度，弯曲强度是 556.35 MPa，和破裂坚韧是 6.73 MPa · m 1/2。 材料的机械性质显然被改进。
An Expert-Level Card Playing Agent Based on a Variant of Perfect Information Monte Carlo Sampling

Despite some success of Perfect Information Monte Carlo Sampling (PIMC) in imperfect information games in the past, it has been eclipsed by other approaches in recent years. Standard PIMC has well-known shortcomings in the accuracy of its decisions, but has the advantage of being simple, fast, robust and scalable, making it well-suited for imperfect information games with large state-spaces. We propose Presumed Value PIMC resolving the problem of overestimation of opponent's knowledge of hidden information in future game states. The resulting AI agent was tested against human experts in Schnapsen, a Central European 2-player trick-taking card game, and performs above human expert-level.
Pathophysiology of ischaemia reperfusion injury: Central role of the neutrophil

Ischaemia is a common clinical event leading to local and remote injury. Evidence indicates that tissue damage is largely caused by activated neutrophils which accumulate when the tissue is reperfused. If the area of ischaemic tissue is large, neutrophils also sequester in the lungs, inducing non‐cardiogenic pulmonary oedema. Ischaemia reperfusion injury is initiated by production of reactive oxygen species which initially appear responsible for the generation of chemotactic activity for neutrophils. Later, once adherent to endothelium, neutrophils mediate damage by secretion of additional reactive oxygen species as well as proteolytic enzymes, in particular elastase. Therapeutic options for limiting ischaemia reperfusion injury include inhibition of oxygen radical formation, pharmacological prevention of neutrophil activation and chemotaxis, and also the use of monoclonal antibodies which prevent neutrophil‐endothelial adhesion, a prerequisite for injury.