Miracle-dz/UniFAR-FTUs huggingface dataset:
Improving methyl ketone production in Escherichia coli by heterologous expression of NADH‐dependent FabG

We previously engineered Escherichia coli to overproduce medium‐ to long‐chain saturated and monounsaturated methyl ketones, which could potentially be applied as diesel fuel blending agents or in the flavor and fragrance industry. Recent efforts at strain optimization have focused on cofactor balance, as fatty acid‐derived pathways face the systematic metabolic challenge of net NADPH consumption (in large part, resulting from the key fatty acid biosynthetic enzyme FabG [β‐ketoacyl‐ACP reductase]) and net NADH production. In this study, we attempted to mitigate cofactor imbalance by heterologously expressing NADH‐dependent, rather than NADPH‐dependent, versions of FabG identified in previous studies. Of the four NADH‐dependent versions of FabG tested in our previously best‐reported methyl ketone‐producing strain (EGS1895), the version from Acholeplasma laidlawii (Al_FabG) showed the greatest increase in methyl ketone yield in shake flasks (35–75% higher than for an RFP negative‐control strain, depending on sugar loading). An improved strain (EGS2920) attained methyl ketone titers during fed‐batch fermentation of 5.4 ± 0.5 g/L, which were, on average, ca. 40% greater than those for the base strain (EGS1895) under fermentation conditions optimized in this study. Shotgun proteomic data for strains EGS2920 and EGS1895 during fed‐batch fermentation were consistent with the goal of alleviating NADPH limitation through expression of Al_FabG. For example, relative to strain EGS1895, strain EGS2920 significantly upregulated glucose‐6‐phosphate isomerase (directing flux into glycolysis rather than the NADPH‐producing pentose phosphate pathway) and downregulated MaeB (a NADP+‐dependent malate dehydrogenase). Overall, the results suggest that heterologous expression of NADH‐dependent FabG in E. coli may improve sustained production of fatty acid‐derived renewable fuels and chemicals.
Modeling unicast device-to-device communications with simuLTE

In LTE-Advanced (LTE-A), device-to-device (D2D) transmissions allow two peering User Equipments to communicate directly without using the Evolved Node-B as relay. D2D is regarded as one of the enablers to bring LTE-A in the context of vehicular networks, smart cities, or M2M applications. Research on this topic is mostly carried out through link-level simulations. In this work, we describe instead the modeling of D2D into a system- level simulator, namely SimuLTE, which enables us to analyze the performance of applications and higher-layer protocols using D2D transmission. We first describe the modeling within the SimuLTE architecture, then we validate it and analyze the performance of D2D communications with frequency reuse.
Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities

An endophytic fungal strain isolated from the leaves of Gymnema sylvestre was identified as Pestalotiopsis microspora VJ1/VS1 based on nucleotide sequencing of internal transcribed spacer region (ITS 1-5.8S-ITS 2) of 18S rRNA gene (NCBI accession number KX213894). In this study, an efficient and ecofriendly approach has been reported for the synthesis of silver nanoparticles (AgNPs) using aqueous culture filtrate of P. microspora. Ultraviolet-visible analysis confirmed the synthesis of AgNPs by showing characteristic absorption peak at 435 nm. Fourier transform infrared spectroscopy analysis revealed the presence of phenolic compounds and proteins in the fungal filtrate, which are plausibly involved in the biosynthesis and capping of AgNPs. Transmission electron microscopy (TEM) showed that the AgNPs were spherical in shape of 2–10 nm in size. Selected area electron diffraction and X-ray diffraction studies determined the crystalline nature of AgNPs with face-centered cubic (FCC) lattice phase. Dynamic light scattering analysis showed that the biosynthesized AgNPs possess high negative zeta potential value of −35.7 mV. Biosynthesized AgNPs were proved to be potential antioxidants by showing effective radical scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl and H2O2 radicals with IC50 values of 76.95±2.96 and 94.95±2.18 µg/mL, respectively. The biosynthesized AgNPs exhibited significant cytotoxic effects against B16F10 (mouse melanoma, IC50 =26.43±3.41 µg/mL), SKOV3 (human ovarian carcinoma, IC50 =16.24±2.48 µg/mL), A549 (human lung adenocarcinoma, IC50 =39.83±3.74 µg/mL), and PC3 (human prostate carcinoma, IC50 =27.71±2.89 µg/mL) cells. The biosynthesized AgNPs were found to be biocompatible toward normal cells (Chinese hamster ovary cell line, IC50 =438.53±4.2 µg/mL). Cytological observations on most susceptible SKOV3 cells revealed concentration-dependent apoptotic changes that include cell membrane blebbing, cell shrinkage, pyknotic nuclei, karyorrhexis followed by destructive fragmentation of nuclei. The results together in this study strongly provided a base for the development of potential and versatile biomedical applications of biosynthesized AgNPs in the near future.
Cerenkov Radiation–Induced Photoimmunotherapy with 18F-FDG

Near-infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with toxicity induced by photoabsorbers after irradiation with NIR light. A limitation of NIR-PIT is the inability to deliver NIR light to a tumor located deep inside the body. Cerenkov radiation (CR) is the ultraviolet and blue light that is produced by a charged particle traveling through a dielectric medium faster than the speed of light in that medium and is commonly produced during radioactive decay. Here, we demonstrate the feasibility of using CR generated by 18F-FDG accumulated in tumors to induce photoimmunotherapy. Methods: Using A431-luc cells, we evaluated the therapeutic effects of CR-PIT in vitro and in vivo using bioluminescence imaging. Results: CR-PIT showed significant suppression of tumor size, but the decrease of bioluminescence after CR-PIT was not observed consistently over the entire time course. Conclusion: Although CR-PIT can induce tumor killing deep within body, it is less effective than NIR-PIT, possibly related to the relatively lower efficiency of short wavelength light than NIR.
Age-related changes in the persistence and pathogenicity of Salmonella typhimurium in chicks.

Effects of age at exposure on the persistence of Salmonella in various tissues of chicks were assessed in two experiments. Broiler chicks, housed on wire floors in isolation cabinets, were orally inoculated with S. typhimurium at various ages (1 to 8 days after hatching). The postinoculation mortality of chicks declined significantly (P less than .05) as the age at inoculation increased. One experiment investigated the effect of age at inoculation on the persistence of S. typhimurium in the cecum. Salmonella persisted for 7 wk after inoculation in 81.3% of the chicks inoculated at 1 day of age and in 62.5% of the chicks inoculated at 8 days of age. The mean number of cecal Salmonella at 7 wk postinoculation was also greater for chicks inoculated on Day 1 than for those inoculated on Day 8. The second experiment examined the effect of age at inoculation on the adherence of S. typhimurium to and penetration through the cecal epithelium. The ceca of chicks inoculated at 1 day of age were colonized by significantly more adhering Salmonella at 2 days postinoculation (1.4 x 10(8)/g) than were those of chicks inoculated at 3, 5, or 7 days of age (8.0 x 16(6)/g or less), but age did not affect the recovery of S. typhimurium from livers or spleens.
Collecting and characterizing a real broadband access network traffic dataset

Broadband Internet access security relies in the implementation of perimeter policies and in the adoption of access control lists. These measures are precarious because they are based on common and not frequently updated profiles that lack residential users threat information. In this paper, we analyze and profile residential users traffic from fixed broadband Internet access networks of a large telecommunication operator for a period of one week, and we obtain the profile of security alarms generated by an intrusion detection system. The results show that the proposed characterization allows the classification of alerts with a sensitivity of 93% in the differentiation of legitimate and anomalous flows and allows a 73% reduction of the traffic directed to the traffic analyzer, thus validating the collected dataset and enabling more dynamic and efficient access network security.
The 24-Hour Effects of Brinzolamide/Brimonidine Fixed Combination and Timolol on Intraocular Pressure and Ocular Perfusion Pressure.

PURPOSE To determine the 24-h effects of brinzolamide/brimonidine tartrate 1%/0.2% fixed combination (BBFC) on intraocular pressure (IOP), ocular perfusion pressure (OPP), blood pressure (BP), and heart rate (HR). METHODS Sixty subjects with open angle glaucoma (OAG) or ocular hypertension (OHTN) were admitted overnight for 24-h monitoring of IOP, BP, and HR. All subjects underwent the first, baseline 24-h study after washout of all medications, if necessary. Subjects were then randomized to receive either (1) timolol maleate 0.5% twice daily or (2) BBFC 3 times daily. After 4 weeks of treatment, all subjects completed a follow-up 24-h study visit. At each study visit, IOP, BP, and HR were measured every 2 h in the habitual position. OPP was calculated as 2/3[diastolic BP +1/3(systolic BP-diastolic BP)]-IOP. RESULTS Treatment with BBFC significantly lowered IOP during the diurnal period (-2.7 ± 0.4 mmHg; P < 0.01) and nocturnal period (-0.8 ± 0.3 mmHg; P < 0.01). Timolol similarly reduced IOP during the diurnal period, but did not lower IOP overnight. Over a 24-h period, BBFC achieved a significantly greater IOP reduction than timolol (-0.7 ± 0.4 mmHg; P = 0.04). BBFC failed to achieve an increase in OPP during any time period, while timolol increased OPP during the diurnal period only. A significantly greater reduction in HR occurred in the timolol group. CONCLUSIONS BBFC significantly lowers IOP during both the diurnal and nocturnal periods, but has no effect on OPP. Timolol only lowers IOP during the diurnal period.
Mode Selection and Spectrum Partition for D2D Inband Communications: A Physical Layer Security Perspective

This paper investigates the fundamental issues of mode selection and spectrum partition in cellular networks with in-band device-to-device (D2D) communication from the physical-layer security (PLS) perspective. We consider a mode selection scheme allowing each D2D pair to probabilistically switch between the underlay and overlay modes, and also a spectrum partition scheme where the system spectrum is orthogonally partitioned between cellular and overlay D2D communications. We first develop a general theoretical framework to model the secrecy outage and secrecy capacity performance of cellular users as well as the outage and capacity performance of D2D pairs. Optimization problems are also solved to identify the optimal mode selection and spectrum partition for secrecy capacity maximization and secrecy outage probability minimization. A case study is then provided to demonstrate the application of our theoretical framework for performance modeling and optimization, and also to illustrate the impacts of mode selection and spectrum partition on the PLS performances of in-band D2D communications.
Einstein@Home discovers a radio-quiet gamma-ray millisecond pulsar

A citizen science project discovers a new type of gamma-ray millisecond pulsar with no detectable radio pulsations. Millisecond pulsars (MSPs) are old neutron stars that spin hundreds of times per second and appear to pulsate as their emission beams cross our line of sight. To date, radio pulsations have been detected from all rotation-powered MSPs. In an attempt to discover radio-quiet gamma-ray MSPs, we used the aggregated power from the computers of tens of thousands of volunteers participating in the Einstein@Home distributed computing project to search for pulsations from unidentified gamma-ray sources in Fermi Large Area Telescope data. This survey discovered two isolated MSPs, one of which is the only known rotation-powered MSP to remain undetected in radio observations. These gamma-ray MSPs were discovered in completely blind searches without prior constraints from other observations, raising hopes for detecting MSPs from a predicted Galactic bulge population.
Refining calcium test for diagnosis of medullary thyroid cancer: cutoffs, procedures and safety

BACKGROUND Prior unblinded studies have suggested that catheter-based renal-artery denervation reduces blood pressure in patients with resistant hypertension. METHODS We designed a prospective, single-blind, randomized, sham-controlled trial. Patients with severe resistant hypertension were randomly assigned in a 2:1 ratio to undergo renal denervation or a sham procedure. Before randomization, patients were receiving a stable antihypertensive regimen involving maximally tolerated doses of at least three drugs, including a diuretic. The primary efficacy end point was the change in office systolic blood pressure at 6 months; a secondary efficacy end point was the change in mean 24-hour ambulatory systolic blood pressure. The primary safety end point was a composite of death, end-stage renal disease, embolic events resulting in end-organ damage, renovascular complications, or hypertensive crisis at 1 month or new renal-artery stenosis of more than 70% at 6 months. RESULTS A total of 535 patients underwent randomization. The mean (±SD) change in systolic blood pressure at 6 months was -14.13±23.93 mm Hg in the denervation group as compared with -11.74±25.94 mm Hg in the sham-procedure group (P<0.001 for both comparisons of the change from baseline), for a difference of -2.39 mm Hg (95% confidence interval [CI], -6.89 to 2.12; P=0.26 for superiority with a margin of 5 mm Hg). The change in 24-hour ambulatory systolic blood pressure was -6.75±15.11 mm Hg in the denervation group and -4.79±17.25 mm Hg in the sham-procedure group, for a difference of -1.96 mm Hg (95% CI, -4.97 to 1.06; P=0.98 for superiority with a margin of 2 mm Hg). There were no significant differences in safety between the two groups. CONCLUSIONS This blinded trial did not show a significant reduction of systolic blood pressure in patients with resistant hypertension 6 months after renal-artery denervation as compared with a sham control. (Funded by Medtronic; SYMPLICITY HTN-3 ClinicalTrials.gov number, NCT01418261.).
Evidence of benefits from using fresh and cryopreserved blood to transfuse patients with acute sickle cell disease

The transfusion of red blood cell (RBC) concentrates is the main treatment for acute vaso‐occlusive symptoms in sickle cell disease (SCD). Units of packed RBCs (pRBCs) must retain optimal characteristics for transfusion throughout the storage period. Transfused RBCs interact with the plasma and the endothelium that lines blood vessels and may be the target of immune‐hematologic conflict if the patient produces antibodies against RBCs. Questions remain concerning the benefit‐risk balance of RBC transfusions, in particular about the shelf‐life of the units.
Toll like receptor signaling pathway as a potential therapeutic target in colorectal cancer

Toll like receptor (TLR) signaling is involved in activating innate and adaptive immune responses and plays a critical role in inflammation‐induced diseases such as colorectal cancer (CRC). Dysregulation of this signaling pathway can result in disturbance of epithelial layer hemostasis, chronic inflammatory, excessive repair responses, and development of CRC. There is now substantial evidence for the benefit of targeting of this pathway in cancer treatment, and several agents have been approved, such as BCG (Bacillus Calmette Guérin), MPL (monophosphoryl lipid A) and imiquimod. This review summarizes the current knowledge about the different functions of TLRs on tumor cells and their application in cancer therapy with particular emphasis on recent preclinical and clinical research in treatment of CRC.
Mean Threshold and ARNN Algorithms for Identification of Eye Commands in an EEG-Controlled Wheelchair

This paper represented Autoregressive Neural Network (ARNN) and meant threshold methods for recognizing eye movements for control of an electrical wheelchair using EEG technology. The eye movements such as eyes open, eyes blinks, glancing left and glancing right related to a few areas of human brain were investigated. A Hamming low pass filter was applied to remove noise and artifacts of the eye signals and to extract the frequency range of the measured signals. An autoregressive model was employed to produce coefficients containing features of the EEG eye signals. The coefficients obtained were inserted the input layer of a neural network model to classify the eye activities. In addition, a mean threshold algorithm was employed for classifying eye movements. Two methods were compared to find the better one for applying in the wheelchair control to follow users to reach the desired direction. Experimental results of controlling the wheelchair in the indoor environment illustrated the effectiveness of the proposed approaches.
EvoCut: A new Generalization of Albert-Barabási Model for Evolution of Complex Networks

With the evolution of social networks, the network structure shows dynamic nature in which nodes and edges appear as well as disappear for various reasons. The role of a node in the network is presented as the number of interactions it has with the other nodes. For this purpose a network is modeled as a graph where nodes represent network members and edges represent a relationship among them. Several models for evolution of social networks has been proposed till date, most widely accepted being the Barabasi-Albert [1] model that is based on preferential attachment of nodes according to the degree distribution. This model leads to generation of graphs that are called Scale Free and the degree distribution of such graphs follow the power law. Several generalizations of this model has also been proposed. In this paper we present a new generalization of the model and attempt to bring out its implications in real life.
Olfactory Function and Associated Clinical Correlates in Former National Football League Players.

Professional American football players incur thousands of repetitive head impacts (RHIs) throughout their lifetime. The long-term consequences of RHI are not well characterized, but may include olfactory dysfunction. RHI has been associated with changes to brain regions involved in olfaction, and olfactory impairment is common after traumatic brain injury. Olfactory dysfunction is a frequent early sequelae of neurodegenerative diseases (e.g., Alzheimer's disease), and RHI is associated with the neurodegenerative disease, chronic traumatic encephalopathy (CTE). We examined olfaction, and its association with clinical measures, in former National Football League (NFL) players. Ninety-five former NFL players (ages 40-69) and 28 same-age controls completed a neuropsychological and neuropsychiatric evaluation as part of a National Institutes of Health-funded study. The Brief Smell Identification Test (B-SIT) assessed olfaction. Principal component analysis generated a four-factor structure of the clinical measures: behavioral/mood, psychomotor speed/executive function, and verbal and visual memory. Former NFL players had worse B-SIT scores relative to controls (p = 0.0096). A B-SIT cutoff of 11 had the greatest accuracy (c-statistic = 0.61) and specificity (79%) for discriminating former NFL players from controls. In the former NFL players, lower B-SIT scores correlated with greater behavioral/mood impairment (p = 0.0254) and worse psychomotor speed/executive functioning (p = 0.0464) after controlling for age and education. Former NFL players exhibited lower olfactory test scores relative to controls, and poorer olfactory test performance was associated with worse neuropsychological and neuropsychiatric functioning. Future work that uses more-comprehensive tests of olfaction and structural and functioning neuroimaging may improve understanding on the association between RHI and olfaction.
Ischemic Stroke Patients Demonstrate Increased Carotid Plaque Microvasculature Compared to (Ocular) Transient Ischemic Attack Patients

Background: Patients with a recent ischemic stroke have a higher risk of recurrent stroke compared to (ocular) transient ischemic attack (TIA) patients. Plaque microvasculature is considered as a feature of plaque vulnerability and can be quantified with carotid dynamic contrast-enhanced MRI (DCE-MRI). The purpose of this cross-sectional study was to explore the association between plaque microvasculature and the type of recent cerebrovascular events in symptomatic patients with mild-to-moderate carotid stenosis. Methods: A total of 87 symptomatic patients with a recent stroke (n = 35) or (ocular) TIA (n = 52) underwent carotid DCE-MRI examination. Plaque microvasculature was studied in the vessel wall and adventitia using DCE-MRI and the pharmacokinetic modeling parameter Ktrans. Statistical analysis was performed with logistic regression, correcting for associated clinical risk factors. Results: The 75th percentile adventitial (OR 1.97, 95% CI 1.18-3.29) Ktrans was significantly associated with a recent ischemic stroke compared to (ocular) TIA in multivariate analysis, while clinical risk factors were not significantly associated with the type of event. Conclusions: This study indicates a positive association of leaky plaque microvasculature with a recent ischemic stroke compared to (ocular) TIA. Prospective longitudinal studies are needed to investigate whether Ktrans or other plaque characteristics may serve as an imaging marker for predicting (the type of) future cerebrovascular events.
Hierarchical Cloth Simulation using Deep Neural Networks

Fast and reliable physically-based simulation techniques are essential for providing flexible visual effects for computer graphics content. In this paper, we propose a fast and reliable hierarchical cloth simulation method, which combines conventional physically-based simulation with deep neural networks (DNN). Simulations of the coarsest level of the hierarchical model are calculated using conventional physically-based simulations, and more detailed levels are generated by inference using DNN models. We demonstrate that our method generates reliable and fast cloth simulation results through experiments under various conditions.
A method of constructing braided Hopf algebras

Let A and B be two Hopf algebras and R ( Hom(B ( A, A ( B), the twisted tensor product Hopf algebra A#RB was introduced by S. Caenepeel et al in [3] and further studied in our recent work [6]. In this paper we give the necessary and sufficient conditions for A#RB to be a Hopf algebra with a projection. Furthermore, a braided Hopf algebra A is constructed by twisting the multiplication of A through a (γ, R)-pair (A, B). Finally we give a method to construct Radford's biproduct directly by defining the module action and comodule action from the twisted tensor biproduct. 2010 Mathematics Subject Classifications. 16W30.
A Survey on Fault-Tolerance in Distributed Network Systems

In this paper, we give a survey on fault tolerant issue in distributed systems. More specially speaking, we talk about one important and basic component called failure detection, which is to detect the failure of the process quickly and accurately. Thus, a good failure detection method will avoid the further system lost due to process crash. This survey provides the related research results and also explored the future directions about failure detection, and it is a good reference for researcher on this topic.