Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic confirmation for a central role for TNFα in the direct action of thyroid stimulating hormone on the skeleton

Clinical data showing correlations between low thyroid-stimulating hormone (TSH) levels and high bone turnover markers, low bone mineral density, and an increased risk of osteoporosis-related fractures are buttressed by mouse genetic and pharmacological studies identifying a direct action of TSH on the skeleton. Here we show that the skeletal actions of TSH deficiency are mediated, in part, through TNFα. Compound mouse mutants generated by genetically deleting the Tnfα gene on a Tshr−/− (homozygote) or Tshr+/− (heterozygote) background resulted in full rescue of the osteoporosis, low bone formation, and hyperresorption that accompany TSH deficiency. Studies using ex vivo bone marrow cell cultures showed that TSH inhibits and stimulates TNFα production from macrophages and osteoblasts, respectively. TNFα, in turn, stimulates osteoclastogenesis but also enhances the production in bone marrow of a variant TSHβ. This locally produced TSH suppresses osteoclast formation in a negative feedback loop. We speculate that TNFα elevations due to low TSH signaling in human hyperthyroidism contribute to the bone loss that has traditionally been attributed solely to high thyroid hormone levels.
New biomechanical model for clinical evaluation of the upper extremity motion in subjects with neurological disorders: an application case

Cervical spinal cord injury and acquired brain injury commonly imply a reduction in the upper extremity function which complicates, or even constrains, the performance of basic activities of daily living. Neurological rehabilitation in specialised hospitals is a common treatment for patients with neurological disorders. This study presents a practical methodology for the objective and quantitative evaluation of the upper extremity motion during an activity of daily living of those subjects. A new biomechanical model (with 10 rigid segments and 20 degrees of freedom) was defined to carry out kinematic, dynamic and energetic analyses of the upper extremity motion during a reaching task through data acquired by an optoelectronic system. In contrast to previous upper extremity models, the present model includes the analysis of the grasp motion, which is considered as crucial by clinicians. In addition to the model, we describe a processing and analysis methodology designed to present relevant summaries of biomechanical information to rehabilitation specialists. As an application case, the method was tested on a total of four subjects: three healthy subjects and one pathological subject suffering from cervical spinal cord injury. The dedicated kinematic, dynamic and energetic analyses for this particular case are presented. The resulting set of biomechanical measurements provides valuable information for clinicians to achieve a thorough understanding of the upper extremity motion, and allows comparing the motion of healthy and pathological cases.
Biological and pharmacological evaluation of dimethoxycurcumin: A metabolically stable curcumin analogue with a promising therapeutic potential

Dimethoxycurcumin (DiMC) is a synthetic analog of curcumin with superior inter‐related pro‐oxidant and anti‐cancer activity, and metabolic stability. Numerous studies have shown that DiMC reserves the biologically beneficial features, including anti‐inflammatory, anti‐carcinogenic, and cytoprotective properties, almost to the same extent as curcumin exhibits. DiMC lacks the phenolic‐OH groups as opposed to curcumin, dimethoxycurcumin, and bis‐demethoxycurcumin that all vary in the number of methoxy groups per molecule, and has drawn the attentions of researchers who attempted to discover the structure‐activity relationship (SAR) of curcumin. In this regard, tetrahydrocurcumin (THC), the reduced and biologically inert metabolite of curcumin, denotes the significance of the conjugated α,β diketone moiety for the curcumin activity. DiMC exerts unique molecular activities compared to curcumin, including induction of androgen receptor (AR) degradation and suppression of the transcription factor activator protein‐1 (AP‐1). The enhanced AR degradation on DiMC treatment suggests it as a novel anticancer agent against resistant tumors with androgenic etiology. Further, DiMC might be a potential treatment for acne vulgaris. DiMC induces epigenetic alteration more effectively than curcumin, although both showed no direct DNA hypomethylating activity. Given the metabolic stability, nanoparticulation of DiMC is more promising for in vivo effectiveness. However, studies in this regard are still in its infancy. In the current review, we portray the various molecular and biological functions of DiMC reported so far. Whenever possible, the efficiency is compared with curcumin and the reasons for DiMC being more metabolically stable are elaborated. We also provide future perspective investigations with respect to varying DiMC‐nanoparticles.
A Low-Power Wide-Range I-V Converter for Amperometric Sensing Applications

This paper presents for the first time experimental results of a current-to-voltage converter that can be used for amperometric sensing of currents ranging from 1 pA to 1 muA. The design strategy is optimized to achieve low power levels and, hence, make the circuit suitable for use in a wearable or implantable sensor. The power reduction is mostly achieved by combining transistors operating in the weak inversion region with floating-gate metal-oxide semiconductor devices and three different gain settings. The power consumption under normal operation is 9.82 muW.
Integrated Questionnaires: Maintaining Presence in Game Environments for Self-Reported Data Acquisition

Research in human-computer interaction often requires the acquisition of self-reported data. Particularly concerning serious games, the interaction between the game and the user still holds many unknown aspects, partly due to the user's double role as player and learner. An easy way of collecting data consists of questionnaires, mostly employed in pen-and-paper or electronic form. In order to gather data points during game play, the player is interrupted, potentially causing unintentional side effects. We suggest an integration of questionnaires into games as game elements, in order to mitigate the effects of interruption. A serious game prototype with an integrated survey was implemented, and evaluated regarding its effects on the players' experience of presence.
The Nature of Progression in Parkinson’s Disease: An Application of Non-Linear, Multivariate, Longitudinal Random Effects Modelling

Background To date, statistical methods that take into account fully the non-linear, longitudinal and multivariate aspects of clinical data have not been applied to the study of progression in Parkinson’s disease (PD). In this paper, we demonstrate the usefulness of such methodology for studying the temporal and spatial aspects of the progression of PD. Extending this methodology further, we also explore the presymptomatic course of this disease. Methods Longitudinal Positron Emission Tomography (PET) measurements were collected on 78 PD patients, from 4 subregions on each side of the brain, using 3 different radiotracers. Non-linear, multivariate, longitudinal random effects modelling was applied to analyze and interpret these data. Results The data showed a non-linear decline in PET measurements, which we modelled successfully by an exponential function depending on two patient-related covariates duration since symptom onset and age at symptom onset. We found that the degree of damage was significantly greater in the posterior putamen than in the anterior putamen throughout the disease. We also found that over the course of the illness, the difference between the less affected and more affected sides of the brain decreased in the anterior putamen. Younger patients had significantly poorer measurements than older patients at the time of symptom onset suggesting more effective compensatory mechanisms delaying the onset of symptoms. Cautious extrapolation showed that disease onset had occurred some 8 to 17 years prior to symptom onset. Conclusions Our model provides important biological insights into the pathogenesis of PD, as well as its preclinical aspects. Our methodology can be applied widely to study many other chronic progressive diseases.
Selective Arterial Calcium Stimulation With Hepatic Venous Sampling in Immune-Mediated Hypoglycemia

The purpose of the current study was to review the biochemical results of selective arterial calcium stimulation (SACST) with hepatic venous sampling in patients with immune-mediated hyperinsulinemic hypoglycemia. A retrospective review was undertaken of four patients with immune-mediated hyperinsulinemic hypoglycemia who underwent SACST with hepatic venous sampling from January 1996 to March 2014. Baseline systemic arterial and hepatic venous insulin concentrations (uIU/mL) were compared, and the absolute and relative-fold increase in hepatic venous insulin concentration after calcium stimulation was calculated. Baseline systemic arterial and hepatic venous insulin concentrations were elevated in all vessels sampled (range, 95 to 1704 uIU/mL), and there was no increase in the absolute or relative (1.0- to 1.3-fold) hepatic venous insulin concentration after calcium injection into any vessel. These data suggest that there are distinct biochemical responses to SACST in patients with immune-mediated hyperinsulinemic hypoglycemia compared with patients with endogenous, pancreatic-mediated hypoglycemia, such as insulinoma or nesidioblastosis.
Longleaf pine characteristics associated with arthropods available for red-cockaded woodpeckers

Red-cockaded woodpeckers (Picoides borealis) forage on the boles of living pine trees for a variety of arthropods. To assess the availability of prey under differing stand conditions, we sampled arthropods that crawled up the boles of 300 living longleaf pine trees (Pinus palustris) ranging in age from 20-100 years with passive traps over a 1-year period. We identified, counted, ovendried, and weighed >50.000 arthropods in 22 orders and 470 genera. The most diverse orders were the Coleoptera (beetles), Araneae (spiders), and Hymenoptera (ants, wasps and bees). The most abundant orders were the Homoptera with large numbers of aphids (Aphididac) and the Hymenoptera with large numbers of ants (Formicidae) The Coleoptera and Araneae accomted for the greatest available biomass. Overall, arthropod biomass/tree increased with increasing stand age up to approximately 65-70 years. Init arthropod biomass/ha was highest in the youngest stands Abundance and biomass of arthropods on each tree bole were positively correlated with bark thickness and tree diameter, and negatively correlated with basal area (m 2 /ha). Arthropod biomass differed among seasons. with the highest arthropod biomass occurring in winter and spring. We found no correlation of diversity. abandance, or biomass of arthropods on the tree bole with site index. the numbers of herbaceous plant genera in the understory, the number of herbaceous plant stems, or the percentage of ground covered by herbs. Stand characteristics, such as average bark thickness and diameter, associated with increased arthropod abundance and biomass on the bark are positively correlated to tree age, but these relationships would change with management practices that either accelerated or slowed tree growth.
Inner and Outer Bounds for the Public Information Embedding Capacity Region Under Multiple Access Attacks

We consider a public multi-user information embedding (watermarking) system in which two messages (watermarks) are independently embedded into two correlated covertexts and are transmitted through a multiple-access attack channel. The tradeoff between the achievable embedding rates and the average distortions for the two embedders is studied. For given distortion levels, inner and outer bounds for the embedding capacity region are obtained in single-letter form. Tighter bounds are also given for independent covertexts.
Thermohaline mixing: a physical mechanism governing the photospheric composition of low-mass giants

Aims. Numerous spectroscopic observations provide compelling evidence for a non-canonical mixing process that modifies the surface abundances of Li, C and N of low-mass red giants when they reach the bump in the luminosity function. Eggleton and collaborators have proposed that a molecular weight inversion created by the 3 He( 3 He, 2p) 4 He reaction may be at the origin of this mixing, and relate it to the Rayleigh-Taylor instability. We argue that one is actually dealing with a double diffusive instability referred to as thermohaline convection and we discuss its influence on the red giant branch. Methods. We compute stellar models of various initial metallicities that include thermohaline mixing, which is treated as a diffusive process based on the prescription given originally by Ulrich for the turbulent diffusivity produced by the thermohaline instability in stellar radiation zones. Results. Thermohaline mixing simultaneously accounts for the observed behaviour of the carbon isotopic ratio and of the abundances of Li, C and N in the upper part of the red giant branch. It significantly reduces the 3 He production with respect to canonical evolution models as required by measurements of 3 He/H in galactic HII regions. Conclusions. Thermohaline mixing is a fundamental physical process that must be included in stellar evolution modeling.
Functional Reorganization of the Motor Cortex in Adult Rats after Cortical Lesion and Treatment with Monoclonal Antibody IN-1

We previously reported anatomical plasticity in the adult motor cortex after a unilateral sensorimotor cortex (SMC) lesion and treatment with monoclonal antibody (mAb) IN-1, which permits neurite outgrowth from the intact, opposite cortex into deafferented subcortical targets. This study was designed to investigate whether treatment with the mAb IN-1 after SMC lesion in the adult leads to functional reorganization of the intact, opposite motor cortex. Adult rats underwent unilateral SMC aspiration lesion and treatment with either mAb IN-1 or control antibody, or no treatment. After a 6 week survival period, the intact, opposite forelimb motor cortex was explored using intracortical microstimulation to evoke forelimb movements. A dramatic increase in ipsilateral movements of the lesion-impaired forelimb was found in animals treated with mAb IN-1 compared with control animals. These results resembled our previous findings of cortical reorganization in the spared hemisphere after neonatal cortical lesion and without any additional treatment. These results show that, after adult cortical lesion, treatment with mAb IN-1 induces a functional reorganization of the intact, opposite motor cortex.
Histones in functional diversification

Recent research suggests that minor changes in the primary sequence of the conserved histones may become major determinants for the chromatin structure regulating gene expression and other DNA‐related processes. An analysis of the involvement of different core histone variants in different nuclear processes and the structure of different variant nucleosome cores shows that this may indeed be so. Histone variants may also be involved in demarcating functional regions of the chromatin. We discuss in this review why two of the four core histones show higher variation. A comparison of the status of variants in yeast with those from higher eukaryotes suggests that histone variants have evolved in synchrony with functional requirement of the cell.
Plasticity of hippocampus following perinatal asphyxia: Effects on postnatal apoptosis and neurogenesis

Asphyxia during delivery produces long‐term deficits in brain development, including hippocampus. We investigated hippocampal plasticity after perinatal asphyxia, measuring postnatal apoptosis and neurogenesis. Asphyxia was performed by immersing rat fetuses with uterine horns removed from ready‐to‐deliver rats into a water bath for 20 min. Caesarean‐delivered pups were used as controls. The animals were euthanized 1 week or 1 month after birth. Apoptotic nuclear morphology and DNA breaks were assessed by Hoechst and TUNEL assays. Neurogenesis was estimated by bromodeoxyuridine/MAP‐2 immunocytochemitry, and the levels and expression of proteins related to apoptosis and cell proliferation were measured by Western blots and in situ hybridization, respectively. There was an increase of apoptosis in CA1, CA3, and dentate gyrus (DG) and cell proliferation and neurogenesis in CA1, DG, and hilus regions of hippocampus 1 week after asphyxia. The increase of apoptosis in CA3 and cell proliferation in the suprapyramidal band of DG was still observed 1 month following asphyxia. There was an increase of BAD, BCL‐2, ERK2, and bFGF levels in whole hippocampus and bFGF expression in CA1 and CA2 and hilus at P7 and P30. There was a concomitant decrease of phosphorylated‐BAD (Ser112) levels. The increase of BAD levels supports the idea of delayed cell death after perinatal asphyxia, whereas the increases of BCL‐2, ERK2, and bFGF levels suggest the activation of neuroprotective and repair pathways. In conclusion, perinatal asphyxia induces short‐ and long‐term regionally specific plastic changes, including delayed cell death and neurogenesis, involving pro‐ and antiapoptotic as well as mitogenic proteins, favoring hippocampal functional recovery. © 2008 Wiley‐Liss, Inc.
Energy reserves mobilization: Strategies of three decapod species

In food deprivation assays, several different responses have been observed in crustaceans. However, studying energy reserves utilization among more than one species during the same starvation period has not yet been performed, particularly to discern whether the responses are due to intrinsic and/or environmental factors. We hypothesize that decapod species with similar feeding habits have the same strategies in the use of energetic reserves during starvation, even though they inhabit different environments. The aim of this study was to compare the energy reserves mobilization of three decapods species (Cherax quadricarinatus, Palaemon argentinus and Munida gregaria) with similar feeding habits, exposed to similar food deprivation conditions. The crayfish, shrimp and squat-lobster were experimentally kept at continuous feeding or continuous starvation throughout 15 days. Every 3rd day, the midgut gland index (MGI), and the glycogen, lipid and protein contents were measured in the midgut gland (MG) and pleon muscle. Palaemon argentinus mobilized more reserves during starvation, followed by C. quadricarinatus, and the last M. gregaria. The starved shrimps presented low MGI, whereas MG showed a reduction in glycogen (from day 6 to 15), lipid (from day 3 to 15), and protein levels (at day 9 and 15) while in their muscle, lipid reserves decreased at days 3 and 6. In C. quadricarinatus, the most affected parameters in the MG were MGI, glycogen (from day 6 to 15), and lipids (at day 12 and 15). In the MG of M. gregaria only the glycogen was reduced during fasting from 3 to 15 days. Even though the three studied species have similar feeding habitats, we found that their energetic profile utilization is different and it could be explained by the habitat, life span, temperature, organ/tissue, and metabolism of the species. Our results may be useful to understand the several different responses of crustaceans during starvation.
Nigropedunculopontine projection in the rat: An Anterograde tracing study with phaseolus vulgaris‐leucoagglutinin (PHA‐L)

The termination of the substantia nigra pars reticulata efferents in the nucleus tegmenti pedunculopontinus was studied in the rat by using the anterograde tracer Phaseolusvulgaris‐leucoagglutinin (PHA‐L). Both large and small injections of PHA‐L in various portions of the substantia nigra pars reticulata labeled varicose fibers in the ipsilateral and contralateral nucleus tegmenti pedunculopontinus, subnucleus dissipatus as well as in the ipsilateral nucleus tegmenti pedunculopontinus, subnucleus compactus. However, the bulk of the nigral fibers appeared to terminate in the medial two‐thirds of the ipsilateral subnucleus dissipatus of the pedunculopontine nucleus and exhibited a discrete dorsoventral topographical pattern. The terminal plexus displayed patches of uneven density, which was partly due to the numerous fiber bundles passing through the pedunculopontine nucleus, but also to an obvious preference of nigral fibers for some cells. Electron microscopic examination confirmed that nearly all of the varicosities observed in the light microscope contained synaptic vesicles and represented either terminal boutons or boutons en passant. The labeled boutons were elongated (average length: 1.5 μm) and consistently contained a prominent group of mitochondria.
FBMC-based air interface for 5G mobile: Challenges and proposed solutions

Scenarios envisaged for mobile communication systems beyond 2020 imply that future applications and services will impose highly diverse requirements on the system design. To appropriately respond to these requirements while ensuring an efficient usage of available spectrum and system resources, the air interface of 5G system is expected to provide much more flexibility compared to today's systems. Filterbank based multi-carrier (FBMC) may be considered a potential enabler for that purpose, as it provides excellent waveform properties with additional degrees of freedom for the system design, thanks to the use of prototype filters that may be adjusted to meet desired system constraints. This paper elaborates on the challenges that still need to be solved to render FBMC an eligible candidate as fundamental technology for 5G mobile radio and presents some first approaches towards practical solutions.
Genetic Fusions of Heat-Labile (LT) and Heat-Stable (ST) Toxoids of Porcine Enterotoxigenic Escherichia coli Elicit Neutralizing Anti-LT and Anti-STa antibodies

ABSTRACT Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of diarrheal disease in humans and farm animals. E. coli fimbriae, or colonization factor antigens (CFAs), and enterotoxins, including heat-labile enterotoxins (LT) and heat-stable enterotoxins (ST), are the key virulence factors in ETEC diarrhea. Unlike fimbriae or LT, STa has not often been included as an antigen in development of vaccines against ETEC diarrhea because of its poor immunogenicity. STa becomes immunogenic only after being coupled with a strongly immunogenic carrier protein. However, native or shorter STa antigens either had to retain toxic activity in order to become antigenic or elicited anti-STa antibodies that were not sufficiently protective. In this study, we genetically mutated the porcine LT (pLT) gene for a pLT192(R→G) toxoid and the porcine STa (pSTa) gene for three full-length pSTa toxoids [STa11(N→K), STa12(P→F), and STa13(A→Q)] and used the full-length pLT192 as an adjuvant to carry the pSTa toxoid for pLT192:pSTa-toxoid fusion antigens. Rabbits immunized with pLT192:pSTa12 or pLT192:pSTa13 fusion protein developed high titers of anti-LT and anti-STa antibodies. Furthermore, rabbit antiserum and antifecal antibodies were able to neutralize purified cholera toxin (CT) and STa toxin. In addition, preliminary data suggested that suckling piglets born by a sow immunized with the pLT192:pSTa13 fusion antigen were protected when challenged with an STa-positive ETEC strain. This study demonstrated that pSTa toxoids are antigenic when fused with a pLT toxoid and that the elicited anti-LT and anti-STa antibodies were protective. This fusion strategy could provide instructive information to develop effective toxoid vaccines against ETEC-associated diarrhea in animals and humans.