Miracle-dz/UniFAR-FTUs huggingface dataset:
A New Parking-Space Detection System Using Prototyping Devices and Bluetooth Low Energy Communication

Parking in crowded city areas usually causes serious problems. Drivers spend an amount of time, effort, and fuel while finding someplace to park their vehicles. Although there are many solutions for free parking space indication; they are only suitable for parking lots inside the buildings, such as the shopping malls. However, there are not many solutions for exterior parking lots and for big cities as a whole. The purpose of this paper is to propose solutions to this problem, especially by considering issues like power consumption and communication approaches. A parking-space occupancy detection system is proposed, utilizing Bluetooth Low Energy communication and Arduino-based sensor devices. Raspberry Pi-based central devices redirect the collected data to the cloud-based database system, which makes them available for drivers to use mobile devices with Android systems. The selected components make the proposed system easily deployable and scalable.
KNAVE II: the definition and implementation of an intelligent tool for visualization and exploration of time-oriented clinical data

KNAVE-II is an intelligent interface to a distributed web-based architecture that enables users (e.g., physicians) to query, visualize and explore clinical time-oriented databases. Based on prior studies, we have defined a set of requirements for provision of a service for interactive exploration of time oriented clinical data. The main requirements include the visualization, interactive exploration and explanation of both raw data and multiple levels of concepts abstracted from these data; the exploration of clinical data at different levels of temporal granularity along both absolute (calendar-based) and relative (clinically meaningful) time-lines; the exploration and dynamic visualization of the effects of simulated hypothetical modifications of raw data on the derived concepts; and the provision of generic services (such as statistics, documentation, fast search and retrieval of clinically significant concepts, amongst others). KNAVE-II has been implemented and is currently evaluated by expert clinicians in several medical domains, such as oncology, involving monitoring of chronic patients.
Independent origins of self‐compatibility in Arabidopsis thaliana

The evolution from outcrossing based on self‐incompatibility (SI) to a selfing system is one of the most prevalent transitions in flowering plants. It has been suggested that the loss of SI in Arabidopsis thaliana is associated with pseudogene formation at the SCR male component of the S locus. Recent work, however, suggests that alternative alleles with large deletions at the S locus are also present and may be responsible for the evolution of self‐compatibility in this species. We demonstrate that most of these deletion alleles are evolutionarily derived from an S haplotype (haplogroups A) that already possessed the SCR pseudogene. This haplotype and its deletion variants are nearly fixed in Europe. Together with previous transgenic data, these results suggest that the pseudogenization of ΨSCR1 gene changed the SI phenotype in the majority of A. thaliana accessions, and was a critical step in the evolution of selfing in this species. Two other haplogroups (B and C) were also identified, the former of which contains a novel and possibly functional SCR allele. In contrast to haplogroups A, these two haplogroups are found primarily in Africa and Asia. These results suggest that self‐compatibility, which appears to be fixed in this species, arose multiple times with different genetic bases, and indicates that a species‐specific trait is associated with parallel evolution at the molecular level.
Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro

Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation. Understanding how to manage this ecosystem to improve human health is an important but challenging goal. Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function. Here, we have investigated whether dietary supplementation—another method used to modulate gut composition and function—could be used to ameliorate the side effects of antibiotics. We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro. Cultures were supplemented with either pectin (a non-fermentable fibre), inulin (a commonly used prebiotic that promotes the growth of beneficial bacteria) or neither. Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics. There is therefore potential for using supplements to lessen the adverse effects of antibiotics. Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
SNR estimation in decibel domain over communication channels

The statistics of the signal estimator for estimating the signal value in decibel (dB) domain proposed in T. Luo and Y.C. Ko (2004), is analyzed. The basic idea of the proposed estimator combines the linear averaging and log domain. Using the most frequently used averaging filter, i.e., sample mean averaging filter, we prove that the performance of the proposed method is very close to the one of the optimum estimator with an assumption of independent identically distributed signal samples under Nakagami fading channel model. Moreover, the proposed method does not need to explicitly estimate the Nakagami parameter, m, which is required for the optimum estimator. For the correlated samples to be used for the estimation, which is a more practical situation, we show from the simulation that the proposed estimator outperforms significantly the optimum minimum variance unbiased estimator obtained under the Rayleigh fading channels. We also show from the simulations that the proposed estimator works well for the multipath fading case and low signal strength.
The benefits and limitations of cell‐free DNA screening for 22q11.2 deletion syndrome

Cell‐free DNA testing is increasingly being used to screen pregnant women for fetal aneuploidy. This technology may also identify microdeletion syndromes, including 22q11.2 deletion syndrome, the most common microdeletion syndrome, and the 22q11.2 duplication syndrome. The purpose of this paper is to provide an overview of the 22q11.2 deletion syndrome, to review the early experience with cell‐free DNA screening for this deletion and to consider the potential benefits that may be associated with prenatal detection of the deletion. © 2016 John Wiley & Sons, Ltd.
Mind Bomb-1 in Dendritic Cells Is Specifically Required for Notch-mediated T Helper Type 2 Differentiation

In dendritic cell (DC)-CD4+ T cell interaction, Notch signaling has been implicated in the CD4+ T cell activation, proliferation, and subset differentiation. However, there has been a lot of debate on the exact role of Notch signaling. Here, we observed that expression of Mind bomb-1 (Mib1), a critical regulator of Notch ligands for the activation of Notch signaling, increases gradually as precursor cells differentiate into DCs in mice. To clarify the role of Mib1 in DC-CD4+ T cell interactions, we generated Mib1-null bone marrow–derived DCs. These cells readily expressed Notch ligands but failed to initiate Notch activation in the adjacent cells. Nevertheless, Mib1-null DCs were able to prime the activation and proliferation of CD4+ T cells, suggesting that Notch activation in CD4+ T cells is not required for these processes. Intriguingly, stimulation of CD4+ T cells with Mib1-null DCs resulted in dramatically diminished Th2 cell populations, while preserving Th1 cell populations, both in vitro and in vivo. Our results demonstrate that Mib1 in DCs is critical for the activation of Notch signaling in CD4+ T cells, and Notch signaling reinforces Th2 differentiation, but is not required for the activation or proliferation of the CD4+ T cells.
Sheep Gait Biomechanics and the Assessment of Musculoskeletal Conditions: A Systematic Review

In recent years, sheep have been reported as the ideal animal model to study osteoporosis, hence it is important to identify instruments, tools and ideal parameters needed to assess the effects of different treatments. In previous studies conducted in other animal models with osteoporosis, the most common parameters used for evaluation concerned primarily bone properties, such as the dual X-ray absorptiometry. However, biomechanical gait analysis as an integrative functional parameter and a non-invasive method, will be an important tool in research and clinical applications. This research review was performed using the PubMed database and included studies related to sheep with outcome measures concerning functional performance assessed during gait in vivo; and excluded studies related with cardiovascular disease and sperm properties, which include other animal species, with outcomes not related with functional locomotor evaluation. Only studies related with bone properties were analyzed. The most frequent and relevant included parameters were the following: mean peak vertical ground force reaction, gait cycle and stance/swing phase duration, percentage of stance/swing phase in a gait cycle, stride length and the stifle joint angles during a gait cycle. Gait biomechanical parameters have been established for the assessment of some clinical orthopedic condition using sheep models but not currently for osteoporosis.
Rapid Movements of Vimentin on Microtubule Tracks: Kinesin-dependent Assembly of Intermediate Filament Networks

The assembly and maintenance of an extended intermediate filament (IF) network in fibroblasts requires microtubule (MT) integrity. Using a green fluorescent protein–vimentin construct, and spreading BHK-21 cells as a model system to study IF–MT interactions, we have discovered a novel mechanism involved in the assembly of the vimentin IF cytoskeleton. This entails the rapid, discontinuous, and MT-dependent movement of IF precursors towards the peripheral regions of the cytoplasm where they appear to assemble into short fibrils. These precursors, or vimentin dots, move at speeds averaging 0.55 ± 0.24 μm/s. The vimentin dots colocalize with MT and their motility is inhibited after treatment with nocodazole. Our studies further implicate a conventional kinesin in the movement of the vimentin dots. The dots colocalize with conventional kinesin as shown by indirect immunofluorescence, and IF preparations from spreading cells are enriched in kinesin. Furthermore, microinjection of kinesin antibodies into spreading cells prevents the assembly of an extended IF network. These studies provide insights into the interactions between the IF and MT systems. They also suggest a role for conventional kinesin in the distribution of non-membranous protein cargo, and the local regulation of IF assembly.
Changes in Cardiovascular Risk Factors During the Perimenopause and Postmenopause and Carotid Artery Atherosclerosis in Healthy Women

Background and Purpose The objectives of this study were to describe the changes in cardiovascular risk factors during the perimenopausal and early postmenopausal years and correlate those changes in risk factors with carotid intimal-medial thickness (IMT) and plaque index measured 5 to 8 years after menopause. Methods Participants were women (n=372) from Allegheny County, Pennsylvania, enrolled in the Healthy Women Study who had been postmenopausal for at least 5 years. Risk factor changes were measured during the perimenopause, ie, between the premenopausal and first year postmenopausal examinations, and during the early postmenopause, ie, between the first and fifth year postmenopausal examinations. Carotid ultrasound scans measured IMT and plaque at examinations 5 to 8 years after menopause among 314 of the women. Results Increases in LDL cholesterol and triglycerides and declines in HDL cholesterol were greater during perimenopause than postmenopause, whereas increases in blood pressure and fasting glucose levels were greater during postmenopause. Premenopausal systolic and pulse pressure, LDL and HDL cholesterol, triglycerides, and body mass index predicted IMT and plaque. Only the change in pulse pressure between premenopausal and first year postmenopausal examinations was related to both IMT and plaque. Conclusions Absolute risk for cardiovascular disease increases substantially in midlife, with a particularly adverse effect on lipid metabolism at the menopause. Premenopausal levels of risk factors are adequate to identify which women should be targeted for intervention.
The Impact of an Objective Structured Teaching Evaluation on Faculty Teaching Skills

Background: Objective structured teaching evaluations (OSTEs) have been utilized to evaluate educational curricula and for resident and faculty development. Purpose: This study examines the impact of an OSTE on faculty teaching effectiveness and faculty satisfaction. Methods: From 2004 to 2007, 46 faculty members participated in the OSTE. Faculty assessed their teaching abilities with a retrospective pre-post-test analysis. Faculty teaching evaluations for the 6 months before and after the OSTE were compared. Faculty participants completed satisfaction questionnaires regarding their OSTE experience and made teaching plans for the future. Results: After the OSTE, faculty reported statistically significant improvements in all self-assessed teaching skills. There was, however, no improvement in their teaching evaluations. Faculty satisfaction with the OSTE experience was high. They indicated teaching plans incorporating lessons from the OSTE. Conclusions: Faculty felt the OSTE was a rewarding experience and reported improvement in their teaching abilities; however, faculty teaching evaluations did not improve.
Modelling and control system design of a small-scale unmanned helicopter

This paper investigates the modelling and control system design of a small-scale unmanned helicopter. We employ a system identification method to get the attitude angular velocity transfer function of a helicopter, and apply a nested PID approach to design the control system, which can be used to control the attitude and position of a helicopter. Experimental results show that the identified model is very accurate and the system designed based on our model can manipulate the attitude and position of a helicopter quite well.
Monolithic Two-Terminal III–V//Si Triple-Junction Solar Cells With 30.2% Efficiency Under 1-Sun AM1.5g

Stacking III-V p-n junctions on top of wafer-based silicon solar cells is a promising way to go beyond the silicon single-junction efficiency limit. In this study, triple-junction GaInP/AlxGa1-xAs//Si solar cells were fabricated using surface-activated direct wafer bonding. Metal-organic-vapor-phase-epitaxy-grown GaInP/AlxGa1-xAs top cells are bonded at low temperature to independently prepared wafer-based silicon cells. n-Si//n-GaAs interfaces were investigated and achieved bulk-like bond strength, high transparency, and conductivity homogeneously over 4-inch wafer area. We used transfer-matrix optical modeling to identify the best design options to reach current-matched two-terminal devices with different mid-cell bandgaps (1.42, 1.47, and 1.52 eV). Solar cells were fabricated accordingly and calibrated under AM1.5g 1-sun conditions. An improved Si back-side passivation process is presented, leading to a current density of 12.4 mA/cm2 (AM1.5g), measured for a flat Si cell below GaAs. The best 4 cm2 GaInP/GaAs//Si triple-junction cell reaches 30.2% 1-sun efficiency.
LMI Stability Conditions for 2D Roesser Models

This note is devoted to the stability analysis of linear two-dimensional systems described by continuous, discrete or mixed Roesser models. Commonly used polynomial-based tests of stability are reduced to that of solving a set of linear matrix inequalities. The main contribution is that the approach is very general and the criteria, under certain hypothesis, are not conservative.
Crystal structure of the complete integrin αVβ3 ectodomain plus an α/β transmembrane fragment

We determined the crystal structure of 1TM-αVβ3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the αV and β3 subunits. 1TM-αVβ3 is more compact and less active in solution when compared with ΔTM-αVβ3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and defines the α–β interface between IE2 (EGF-like 2) and the thigh domains. Modifying this interface by site-directed mutagenesis leads to robust integrin activation. Fluorescent lifetime imaging microscopy of inactive full-length αVβ3 on live cells yields a donor–membrane acceptor distance, which is consistent with the bent conformation and does not change in the activated integrin. These data are the first direct demonstration of conformational coupling of the integrin leg and head domains, identify the IE2–thigh interface as a critical steric barrier in integrin activation, and suggest that inside-out activation in intact cells may involve conformational changes other than the postulated switch to a genu-linear state.
Localization of the active site of human tumour necrosis factor (hTNF) by mutational analysis.

In order to define the active site(s) of human tumour necrosis factor (hTNF), we mutagenized its gene at random and directly screened the resulting population for loss of cytotoxic activity on L929 cells. Four biologically inactive mutant proteins (Arg32‐‐‐‐Trp, Leu36‐‐‐‐Phe, Ser86‐‐‐‐Phe and Ala84‐‐‐‐Val) behaved similar to the wild‐type in various physico‐chemical assays. The residues were positioned on a 3D structural model and were found to cluster together at the base of the molecule at each side of the groove that separates two monomers in the trimeric structure. A very conservative mutation at one of these sites (Ala84‐‐‐‐Val) almost completely abolished cytotoxic activity. Amino acid alterations in three other residues in close proximity to this receptor binding site were introduced: replacements at positions 29 and 146 clearly reduced cytotoxicity only when non‐conservative alterations were introduced (Leu29‐‐‐‐Ser and Glu146‐‐‐‐Lys), suggesting an indirect influence on the active site. However, a conservative mutation at position 91 (Val‐‐‐‐Ala) caused a significant drop (500‐fold) in bioactivity which suggests that Val91 may also play a direct role in receptor recognition. Our results favor a model in which each TNF molecule has three receptor‐interaction sites (between the three subunits), thus allowing signal transmission by receptor clustering.
Cancer Clocks Out for Lunch: Disruption of Circadian Rhythm and Metabolic Oscillation in Cancer

Circadian rhythms are 24-h oscillations present in most eukaryotes and many prokaryotes that synchronize activity to the day-night cycle. They are an essential feature of organismal and cell physiology that coordinate many of the metabolic, biosynthetic, and signal transduction pathways studied in biology. The molecular mechanism of circadian rhythm is controlled both by signal transduction and gene transcription as well as by metabolic feedback. The role of circadian rhythm in cancer cell development and survival is still not well understood, but as will be discussed in this Review, accumulated research suggests that circadian rhythm may be altered or disrupted in many human cancers downstream of common oncogenic alterations. Thus, a complete understanding of the genetic and metabolic alterations in cancer must take potential circadian rhythm perturbations into account, as this disruption itself will influence how gene expression and metabolism are altered in the cancer cell compared to its non-transformed neighbor. It will be important to better understand these circadian changes in both normal and cancer cell physiology to potentially design treatment modalities to exploit this insight.
A framework for understanding visits by frequent attenders in family practice.

OBJECTIVE Our purpose was to develop a typology of outpatient visits between family physicians and adult "frequent attender" patients. STUDY DESIGN This was a cross-sectional observational study using qualitative analysis of family physician visits. Three family physician researchers reviewed detailed field notes for each patient based on direct observation of a single office visit to determine major themes and characteristics of physician-patient encounters. POPULATION Non-pregnant adults in the top 5% for visit frequency, and age-and sex-mated non-frequent attenders were identified from among 1194 adult patients in 18 Midwestern family practice offices as part of The Prevention and Competing Demands in Primary Care Study. RESULTS Visits by 62 patients who had made at least 25 visits in the previous 2 years were selected (frequent attender visits). Three major dimensions emerged to distinguish different encounter types: (1) biomedical complexity, (2) psychosocial complexity, and (3) the degree of dissonance between the patient and the physician. These 3 dimensions were used in a descriptive framework to characterize visit types as: simple medical, ritual visit, complicated medical, the tango, simple frustration, psychosocial disconnect, medical disharmony, and the heartsink visit. CONCLUSIONS The discovery of a wide variation of encounter types among adult frequent attenders and the resulting descriptive framework laid a foundation for defining the appropriateness of outpatient health care utilization, for designing interventions to reduce inappropriate utilization, and for educating physicians regarding effective management of frequent attender patients.
Estimating exotic gene flow into native pine stands: zygotic vs. gametic components

Monitoring contemporary gene flow from widespread exotic plantations is becoming an important problem in forest conservation genetics. In plants, where both seed and pollen disperse, three components of exotic gene flow with potentially unequal consequences should be, but have not been, explicitly distinguished: zygotic, male gametic and female gametic. Building on a previous model for estimating contemporary rates of zygotic and male gametic gene flow among plant populations, we present here an approach that additionally estimates the third (female gametic) gene flow component, based on a combination of uni‐ and biparentally inherited markers. Using this method and a combined set of chloroplast and nuclear microsatellites, we estimate gene flow rates from exotic plantations into two Iberian relict stands of maritime pine (Pinus pinaster) and Scots pine (Pinus sylvestris). Results show neither zygotic nor female gametic gene flow but moderate (6–8%) male gametic introgression for both species, implying significant dispersal of pollen, but not of seeds, from exotic plantations into native stands shortly after introduced trees reached reproductive maturity. Numerical simulation results suggest that the model yields reasonably accurate estimates for our empirical data sets, especially for larger samples. We discuss conservation management implications of observed levels of exposure to nonlocal genes and identify research needs to determine potentially associated hazards. Our approach should be useful for plant ecologists and ecosystem managers interested in the vectors of contemporary genetic connectivity among discrete plant populations.