Miracle-dz/UniFAR-FTUs huggingface dataset:
Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or “wobble,” position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.
New immunological assays for the diagnosis ofHelicobacter pyloriinfection

There are several types of immunological tests available for the diagnosis and management of Helicobacter pylori infection. Most commercially available serological kits use the enzyme linked immunosorbent assay (ELISA) test format. Originally the kits used crude antigen preparations although many of the newer kits use a more purified antigen preparation, with often increased specificity but lower sensitivity. Near patient test kits are based either on latex agglutination or immunochromatography. Generally they have low sensitivities compared with laboratory tests. Western blotting, ELISA, and recombinant immunoblot assays (RIBA) have also been developed into commercially available kits and can be used to indicate the presence of specific virulence markers. An antigen detection kit has been developed for the detection of Helicobacter pylori in faeces. Immunological reagents have also been combined with other diagnostic modalities to develop immunohistochemical stains and DNA immunoassays. Helicobacter pylori is now recognised as the cause of gastritis and most cases of peptic ulcer disease (PUD); its long term carriage increases the risk of gastric adenocarcinoma sixfold and it is designated as a class I carcinogen. H pylori has also been implicated as a cause of gastric mucosa associated lymphoid tissue lymphomas. Its relation to non-ulcer dyspepsia remains controversial. Additionally, long term carriage of the organism may be associated with short stature in young girls and, in the general population, as a possible risk factor for the development of vasospastic disorders and possibly skin immunopathology such as urticaria. With the recognition of H pylori as an important human pathogen, it has become one of the growing number of organisms to have its complete genome sequence mapped. Serology is an important method of determining colonisation status and can be used for diagnosis, as a screening procedure, or to follow the efficacy of eradication regimens. Most serological assays are in the ELISA format although some are based on the latex agglutination reaction. These latter are used principally as near patient assays. Most assays detect IgG in serum although some detect serum IgA. More recently developed assays detect IgA in saliva and the production of affinity purified antibodies has led to the development of an antigen detection assay for faecal specimens. Serological reagents have also been used in immunocytochemistry and to speed up the detection of amplified products of the polymerase chain reaction (PCR)-DNA immunoassays.
PaceMaker: When ZooKeeper Arteries Get Clogged in Storm Clusters

As the use of Apache Storm, a distributed real-time computation platform, becomes more widespread in community, scalability of the platform has become a key challenge. Currently, Apache Storm leverages Apache Zookeeper as a message broker for heartbeats and metrics. Using Apache Zookeeper in this manner may have been a good implementation choice at the inception of Apache Storm since it leverages the use of an existing proven open source technology instead of having to implement a proprietary message broker for Storm. However, using Apache Zookeeper in such a fashion has created one of the biggest bottlenecks in scaling Storm. In this paper, we first explore the key scalability issues that this architecture was creating as cluster and workload sizes grow at Yahoo. Next, we discuss in detail a new heartbeat and metrics service, called PaceMaker to address this scalability problem. Finally, we present experimental results to demonstrate that PaceMaker greatly relieves the workload and resource pressure on the ZooKeeper nodes which enables Storm to scale much further.
Performance analysis of isolated 3-phase self excited induction generator using graph theory and PSO technique

This paper analyzes the steady state performance of isolated 3-phase self excited induction generator (SEIG) feeding balanced static resistive load. The effects of load and capacitance on magnetizing reactance, frequency, terminal voltage, currents have been shown in this paper. The model equations, which are nonlinear in nature, have been developed using graph theory. These equations can be easily solved by particle swarm optimization method. The performance of this algorithm has been compared with Newton-Raphson method. MATLAB has been used for simulation. The simulated results obtained using these algorithms help in understanding the steady state performance of SEIG.
Insulin and Fibroblast Growth Factor 2 Activate a Neurogenic Program in Müller Glia of the Chicken Retina

We have reported previously that neurotoxic damage to the chicken retina causes Müller glia to dedifferentiate, proliferate, express transcription factors common to retinal progenitors, and generate new neurons and glia, whereas the majority of newly produced cells remain undifferentiated (Fischer and Reh, 2001). Because damaged retinal cells have been shown to produce increased levels of insulin-related factors and FGFs, in the current study we tested whether intraocular injections of growth factors stimulate Müller glia to proliferate and produce new neurons. We injected growth factors and bromodeoxyuridine into the vitreous chamber of the eyes of chickens and assayed for changes in glial phenotype and proliferation within the retina. Although insulin or FGF2 alone had no effect, the combination of insulin and FGF2 caused Müller glia to coexpress transcription factors common to retinal progenitors (Pax6 and Chx10) and initiated a wave of proliferation in Müller cells that began at the retinal margin and spread into peripheral regions of the retina. Most of the newly formed cells remain undifferentiated, expressing Pax6 and Chx10, whereas some differentiate into Müller glia, and a few differentiate into neurons that express the neuronal markers Hu or calretinin. There was no evidence of retinal damage in eyes treated with insulin and FGF2. We conclude that the combination of insulin and FGF2 stimulated Müller glia to dedifferentiate, proliferate, and generate new neurons. These findings imply that exogenous growth factors might be used to stimulate endogenous glial cells to regenerate neurons in the CNS.
An Imperceptible Difference: Visual and Textual Constructions of Femininity in Sports Illustrated and Sports Illustrated for Women

The success of female athletes in the 1996 Olympics brought with it a great deal of optimism that women in sports would finally receive acceptance for their athletic talents. This optimism was concomitantly fueled by the rise of women's sport magazines. This study was designed with 2 purposes. First, through an analysis of both visual and literal texts, we sought to replicate previous research in determining whether there had been any changes in the coverage of female sports and athletes in Sports Illustrated's historically male-centered magazine. Second, the same standards of review were applied to Sports Illustrated for Women to discover if the mandates for marketing femininity are so strong that they have crossed over to this female-specific sport magazine as well. An analysis of content (1,105 articles and 1,745 photographs) within Sports Illustrated and Sports Illustrated for Women from 1997 to 1999 found that women continue to be underrepresented, portrayed in traditionally feminine sports, or shown in nonsport-related scenery in both media outlets. Within the pages of media explicitly focused on women's issues within sports, successful female athletes continue to be constructed in stereotypical and traditional conceptions of femininity that supercede their athletic ability. It is suggested that this generally unoffensive, status-quo approach has been continued to maintain marketability to advertisers and to general sports readers.
METAPHOR IN PSYCHOANALYTIC PSYCHOTHERAPY: A COMPARATIVE STUDY OF FOUR CASES BY A PRACTITIONER–RESEARCHER

Twelve transcripts, three from each of four patients, taken from the early, mid and late phases of open-ended, long-term psychoanalytic psychotherapy with the same therapist, were analysed for metaphor. Metaphors were categorized into key, novel and conventional. Using grounded theory metaphors were categorized by theme and function. It was found that all metaphors – novel, conventional and key – could be representative of key concepts such as the self, others/relationships and therapy/self transformation and diagnosis or psychopathology; chart change; indicate mentalization (Fonagy et al. 2004). In addition use of metaphors by different patients showed different patterns that co-occurred with good or bad outcome.
A converter to interface ultra-capacitor energy storage to a fuel cell system

This paper describes a DC-DC power converter for charging and discharging an ultra-capacitor module within a small, sustainable energy system. The energy source in the system is a fuel cell, and a bidirectional grid-connected inverter supplies local AC loads, exports excess energy to the grid, and can import energy if required to charge the capacitors. The capacitor charger provides an interface between the near-constant voltage of a common DC bus and the widely varying ultra-capacitor voltage. The fuel cell system is normally operated with a battery bank at it's output. Therefore, the particular feature of the converter is that it provides closed-loop voltage control of the common DC bus. in order to emulate simplified voltage-current terminal characteristics of batteries.
Novel algorithm for landmine detection using C-scan ground penetrating radar signals

This paper introduces a novel algorithm that can achieve close-range landmine detection using Ground Penetrating Radar (GPR) data. The proposed GPR feature-based algorithm combines spectral and spatial edge features of the GPR signals to form a new oscillation feature, which can represent the reflection value change between the layers of a C-scan GPR image. The GPR feature-based algorithm detects landmines through comparing the oscillation feature of each A-scan GPR signal in the 3D GPR image to generate a 2D landmine detection result image. Performance evaluation results of the proposed algorithm in comparison with existing methods are reported in terms of detection sensitivity, false alarm rate and computational complexity. The test results show that the GPR feature-based algorithm achieves a high detection performance and can be used in real-time landmine detecting systems.
Amplitude equations for a linear wave equation in a weakly curved pipe

We study boundary effects in a linear wave equation with Dirichlet-type conditions in a weakly curved pipe. The coordinates in our pipe are prescribed by a given small curvature with finite range, with the pipe's cross section being circular. Based on the straight pipe case, a perturbative analysis by which the boundary value conditions are exactly satisfied is employed. As such an analysis, we decompose the wave equation into a set of ordinary differential equations perturbatively. We show the conditions when secular terms due to the curved boundary appear in the naive peturbative analysis. In eliminating such a secularity with a singular perturbation method, we derive amplitude equations and show that the eigenfrequencies in time are shifted due to the curved boundary.
Automated Large-Scale Culture and Medium-Throughput Chemical Screen for Modulators of Proliferation and Viability of Human Induced Pluripotent Stem Cell–Derived Neuroepithelial-like Stem Cells

The aim of this study was to demonstrate proof-of-concept feasibility for the use of human neural stem cells (NSCs) for high-throughput screening (HTS) applications. For this study, an adherent human induced pluripotent stem (iPS) cell–derived long-term, self-renewing, neuroepithelial-like stem (lt-NES) cell line was selected as a representative NSC. Here, we describe the automated large-scale serum-free culture (“scale-up”) of human lt-NES cells on the CompacT SelecT cell culture robotic platform, followed by their subsequent automated “scale-out” into a microwell plate format. We also report a medium-throughput screen of 1000 compounds to identify modulators of neural stem cell proliferation and/or survival. The screen was performed on two independent occasions using a cell viability assay with end-point reading resulting in the identification of 24 potential hit compounds, 5 of which were found to increase the proliferation and/or survival of human lt-NES on both occasions. Follow-up studies confirmed a dose-dependent effect of one of the hit compounds, which was a Cdk-2 modulator. This approach could be further developed as part of a strategy to screen compounds to either improve the procedures for the in vitro expansion of neural stem cells or to potentially modulate endogenous neural stem cell behavior in the diseased nervous system.
Seamless transition of three phase microgrid with load compensation capabilities

This, paper presents the grid synchronization of photovoltaic (PV)-battery based microgrid with multifunctional capabilities. The proposed system is capable to work in the grid integrated mode as well as in islanded mode, based on the grid availability. The objective of this work is a seamless transition between two operating modes of a three phase microgrid. Besides, capability of reconfiguration, it integrates maximum extracted PV power to the utility by utilizing PV feed — forward loop (PVFFL) and suppresses the harmonics currents of nonlinear loads connected to a microgrid. Moreover, an incorporation of PVFFL improves the dynamic response of three phase microgrid under variable climate conditions. The mandatory control structures (voltage control and current control) of voltage source converter (VSC) are utilized together with synchronization control to synchronize a three phase microgrid to the utility. Tests results illustrate a seamless transition and other operating conditions of three phase microgrid.
Acute symptomatic seizures—Should we retain the term?

League Against Epilepsy. (1981) Proposal for revised clinical and electrographic classification of epileptic seizures. Epilepsia. 22:489– 501. Commission on Classification and Terminology of the International League Against Epilepsy. (1989) Proposal for revised classification of epilepsies and epileptic syndromes. Epilepsia. 30:389–399. Engel J Jr. (2001) A proposed scheme for people with epileptic seizures and with epilepsy: report of the ILAE task force on classification and terminology. Epilepsia 42:796–803. Meeren HP, Pijn JP, Van Luijtelaar EL, Coenen AM, Lopes da Silva FG. (2002) Cortical focus drives widespread corticothalamic networks during spontaneous absence seizures in rats. J Neurosci 15:1480– 1495. Polack PO, Guillemain I, Hu E, Deransart C, Depaulis A, Charpier S. (2007) Deep layer somatosensory cortical neurons initiate spike-andwave discharges in a genetic model of absence seizures. J Neurosci 27:6590–6599. Wolf P. (2006) Basic principles of the ILAE syndrome classification. Epilepsy Res 70:S20–S26.
Gravity content in dreams

Flying and falling dreams are often listed among the most common of dreams. Aside from the pure form, in which the dreamer actually falls or flies, it is frequent to find situations in which the dreamer deals with actions or objects implying gravity functions, that is, climbing, floating through air or water, and going up or down on a ladder or in an elevator. By means of the analysis of 685 dreams of male and female subjects, aged between 10 and 32, we registered various gravity contents (falling, flying, water, climbing, descending, staircase, and elevator) and their interrelations. Results show the presence of these elements in 38.1% of the sampled dreams. The authors focused on the link between gravity contents and other typical elements appearing in the same dream (attack, loss, sexual relationships, the body, performance/exams, and nursing). Results tend to confirm a link between gravity content and sexuality. The results of our research are essentially consistent with the findings of an analysis in DreamBank (http://www. dreambank.net/) of the frequency of words related to gravity in a dreams sample (N ! 14,193).
Ribosomal S6 Kinase 2 Directly Phosphorylates the 5-Hydroxytryptamine 2A (5-HT2A) Serotonin Receptor, Thereby Modulating 5-HT2A Signaling*

The 5-hydroxytryptamine 2A (5-HT2A) receptor is a member of the G protein-coupled receptor superfamily (GPCR) and plays a key role in transducing a variety of cellular signals elicited by 5-hydroxytryptamine in both peripheral and central tissues. Despite its broad physiological importance, our current understanding of 5-HT2A receptor regulation is incomplete. We recently reported the novel finding that the multifunctional ERK effector ribosomal S6 kinase 2 (RSK2) physically interacts with the 5-HT2A receptor third intracellular (i3) loop and modulates receptor signaling (Sheffler, D. J., Kroeze, W. K., Garcia, B. G., Deutch, A. Y., Hufeisen, S. J., Leahy, P., Bruning, J. C., and Roth, B. L. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 4717–4722). We report here that RSK2 directly phosphorylates the 5-HT2A receptor i3 loop at the conserved residue Ser-314, thereby modulating 5-HT2A receptor signaling. Furthermore, these studies led to the discovery that RSK2 is required for epidermal growth factor-mediated heterologous desensitization of the 5-HT2A receptor. We arrived at these conclusions via multiple lines of evidence, including in vitro kinase experiments, tandem mass spectrometry, and site-directed mutagenesis. Our findings were further validated using phospho-specific Western blot analysis, metabolic labeling studies, and whole-cell signaling experiments. These results support a novel regulatory mechanism in which a downstream effector of the ERK/MAPK pathway directly interacts with, phosphorylates, and modulates signaling of the 5-HT2A serotonin receptor. To our knowledge, these findings are the first to demonstrate that a downstream member of the ERK/MAPK cascade phosphorylates a GPCR as well as mediates cross-talk between a growth factor and a GPCR.
Parametric Trojans for Fault-Injection Attacks on Cryptographic Hardware

We propose two extremely stealthy hardware Trojans that facilitate fault-injection attacks in cryptographic blocks. The Trojans are carefully inserted to modify the electrical characteristics of predetermined transistors in a circuit by altering parameters such as doping concentration and do pant area. These Trojans are activated with very low probability under the presence of a slightly reduced supply voltage (0.001 for 20% Vdd reduction). We demonstrate the effectiveness of the Trojans by utilizing them to inject faults into an ASIC implementation of the recently introduced lightweight cipher PRINCE. Full circuit-level simulation followed by differential cryptanalysis demonstrate that the secret key can be reconstructed after around 5 fault-injections.
An Analysis of Team Evolution and Maturation

Abstract Although there has been little research emphasis on the temporal aspects of team development, conventional wisdom has suggested that teams develop in a linear fashion through a sequence of phases. More recent investigations of work groups indicate, however, that teams develop through a variety of alternative paths rather than a single sequence of developmental phases. These lines of thinking are integrated here in the form of a model called Team Evolution And Maturation (TEAM), which helps to guide consideration of the development of team performance. The results of an experimental investigation are presented as a preliminary test of the model's suggestion that team development is characterized by the differential maturation of taskwork and teamwork skills. Results indicate that task- and team-related activities were distinguishable in the middle phases of training, but not at the beginning and end of training. These findings are discussed in terms of their implications for investigations of team.
Ebola Viral Disease in West Africa: A Threat to Global Health, Economy and Political Stability

The West African sub-continent is currently experiencing its first, and ironically, the largest and longest Ebola viral diseases (EVD) outbreak ever documented in modern medical history. The current outbreak is significant in several ways, including longevity, magnitude of morbidity and mortality, occurrence outside the traditional niches, rapid spread and potential of becoming a global health tragedy. The authors provided explicit insights into the current and historical background, drivers of the epidemic, societal impacts, status of vaccines and drugs development and proffered recommendations to halt and prevent future occurrences. The authors reviewed mainly five databases and a hand search of key relevant literature. We reviewed 51 articles that were relevant up until the 18th of August 2014. The authors supplemented the search with reference list of relevant articles and grey literature as well as relevant Internet websites. Article searches were limited to those published either in English or French. There are strong indications that the EVD may have been triggered by increased human activities and encroachment into the forest ecosystem spurred by increasing population and poverty-driven forest-dependent local economy. Containment efforts are being hampered by weak and fragile health systems, including public health surveillance and weak governance, certain socio-anthropological factors, fast travels (improved transport systems) and globalization. The societal impacts of the EBV outbreak are grave, including economic shutdown, weakening of socio-political systems, psychological distress, and unprecedented consumption of scarce health resources. The research and development (R&D) pipeline for product against EBV seems grossly insufficient. The outbreak of Ebola and the seeming difficulty to contain the epidemic is simply a reflection of the weak health system, poor surveillance and emergency preparedness/response, poverty and disconnect between the government and the people in many West African countries. Although interventions by the United Nations and other international development agencies could ultimately halt the epidemic, local communities must be engaged to build trust and create demand for the public health interventions being implemented in the Ebola-ravaged populations. In the intermediate and long term, post-Ebola rehabilitation should focus on strengthening of health systems, improving awareness about zoonosis and health behaviors, alleviating poverty and mitigating the impact of triggering factors. Finally, national governments and international development partners should mobilize huge resources and investments to spur or facilitate R&D of disease control tools for emerging and pernicious infectious diseases (not limited to EVD).