Miracle-dz/UniFAR-FTUs huggingface dataset:
A theoretical and experimental investigation of the phase configuration of internal waves of small amplitude in a density stratified liquid

Experiments were conducted to test the linear theory of internal gravity waves produced in a stably stratified liquid by the forced oscillations and the initial impulsive motion of a two-dimensional stationary disturbance. The measurements of the wave configuration in a medium whose density increased linearly with depth were made by means of a Toepler-schlieren system. The agreement between observation and prediction was found to be good.
The Anti-Tumor Effect of the Newly Developed LAT1 Inhibitor JPH203 in Colorectal Carcinoma, According to a Comprehensive Analysis

Simple Summary The large neutral amino acid transporter family (LAT1-4) plays an important role in the intracellular uptake of essential amino acids. Among them, LAT1 is specifically expressed in cancer cells. A novel LAT1-specific inhibitor, JPH203, is expected to cause cancer-specific starvation and possess anti-tumor effects; however, the anti-tumor mechanism for colorectal cancer (CRC) remains unclear. In this study, we clarified the significance of LAT1 expression in cancer progression in database analysis and clinical specimens and demonstrated the utility of JPH203, in vitro and in vivo, using a unique stroma-abundant allogeneic immunoreactive mouse colorectal tumor created by the orthotopic transplantation of a mouse-derived CRC cell line and mesenchymal stem cells into mouse cecum. Comprehensive analysis with RNA sequences showed the efficacy of JPH203 not only in cancer proliferation, but also in tumor stromal activation. These findings will be useful when considering its application in other stroma-abundant carcinomas and its use in combination with other drugs. Abstract A novel large neutral amino acid transporter 1 (LAT1)-specific inhibitor, JPH203, is expected to cause cancer-specific starvation and possess anti-tumor effects; however, its anti-tumor mechanism for colorectal cancer (CRC) remains unclear. We analyzed LAT family gene expressions in public databases using UCSC Xena and evaluated LAT1 protein expression using immunohistochemistry in 154 cases of surgically resected CRC. We also evaluated mRNA expression using polymerase chain reaction in 10 CRC cell lines. Furthermore, JPH203 treatment experiments were conducted in vitro and in vivo using an allogeneic immune-responsive mouse model with abundant stroma created via the orthotopic transplantation of the mouse-derived CRC cell line CT26 and mesenchymal stem cells. The treatment experiments were followed by comprehensive gene expression analyses with RNA sequencing. Database analyses and immunohistochemistry research on clinical specimens revealed that LAT1 expression was cancer-dominant, and its increase was accompanied by tumor progression. In vitro, JPH203 was effective in an LAT1 expression-dependent manner. In vivo, JPH203 treatment considerably reduced tumor size and metastasis, and RNA sequencing-based pathway analysis showed that not only tumor growth and amino acid metabolism pathways, but also stromal activation-related pathways were suppressed. The results of the RNA sequencing were validated in the clinical specimens, as well as both in vitro and in vivo. LAT1 expression in CRC plays an important role in tumor progression. JPH203 may inhibit the progression of CRC and tumor stromal activity.
A Review of the Thermochemical Behaviour of Fluxes in Submerged Arc Welding: Modelling of Gas Phase Reactions

This review is focused on the thermochemical behaviour of fluxes in submerged arc welding (SAW). The English-language literature from the 1970s onwards is reviewed. It was recognised early on that the thermochemical behaviour of fluxes sets the weld metal total ppm O and the element transfer extent from the molten flux (slag) to the weld pool. Despite the establishment of this link between the flux-induced oxygen potential and element transfer, it is also well accepted that the slag–metal equilibrium does not control SAW process metallurgy. Instead, the gas phase must be taken into account to better describe SAW process metallurgy equilibrium calculations. This is illustrated in the gas–slag–metal equilibrium simulation model developed by Coetsee. This model provides improved accuracy in predicting the weld metal total ppm O values as compared to the empirical trend of Tuliani et al. Recent works on the application of Al metal powder with alloying metal powders provide new insights into the likely gas phase reactions in the SAW process and the modification of the flux oxygen behaviour via Al additions. Aluminium may lower the partial oxygen pressure in the arc cavity, and aluminium also lowers the partial oxygen pressure at the weld pool–slag interface. The weld metal total ppm O is lowered with the addition of aluminium in SAW, but not to the same extent as would be expected from steelmaking ladle metallurgy de-oxidation practice when using Al as de-oxidiser. This difference indicates that slag–metal equilibrium is not maintained in the SAW process.
Genomic Diversity, Antimicrobial Susceptibility, and Biofilm Formation of Clinical Acinetobacter baumannii Isolates from Horses

Acinetobacter (A.) baumannii is an opportunistic pathogen that causes severe infections in humans and animals, including horses. The occurrence of dominant international clones (ICs), frequent multidrug resistance, and the capability to form biofilms are considered major factors in the successful spread of A. baumannii in human and veterinary clinical environments. Since little is known about A. baumannii isolates from horses, we studied 78 equine A. baumannii isolates obtained from clinical samples between 2008 and 2020 for their antimicrobial resistance (AMR), clonal distribution, biofilm-associated genes (BAGs), and biofilm-forming capability. Based on whole-genome sequence analyses, ICs, multilocus (ML) and core-genome ML sequence types (STs), and AMR genes were determined. Antimicrobial susceptibility testing was performed by microbroth dilution. A crystal violet assay was used for biofilm quantification. Almost 37.2% of the isolates were assigned to IC1 (10.3%), IC2 (20.5%), and IC3 (6.4%). Overall, the isolates revealed high genomic diversity. We identified 51 different STs, including 22 novel STs (ST1723–ST1744), and 34 variants of the intrinsic oxacillinase (OXA), including 8 novel variants (OXA-970 to OXA-977). All isolates were resistant to ampicillin, amoxicillin/clavulanic acid, cephalexin, cefpodoxime, and nitrofurantoin. IC1-IC3 isolates were also resistant to gentamicin, enrofloxacin, marbofloxacin, tetracycline, and trimethoprim/sulfamethoxazole. All isolates were susceptible to imipenem. Thirty-one multidrug-resistant (MDR) isolates mainly accumulated in the IC1-IC3 groups. In general, these isolates showed less biofilm formation (IC1 = 25.0%, IC2 = 18.4%, IC3 = 15.0%) than the group of non-IC1-IC3 isolates (58.4%). Isolates belonging to the same ICs/STs revealed identical BAG patterns. BAG blp1 was absent in all isolates, whereas bfmR and pgaA were present in all isolates. At the level of the IC groups, the AMR status was negatively correlated with the isolates’ ability to form a biofilm. A considerable portion of equine A. baumannii isolates revealed ICs/STs that are globally present in humans. Both an MDR phenotype and the capability to form biofilms might lead to therapeutic failures in equine medicine, particularly due to the limited availability of licensed drugs.
Integrative Nomogram of Computed Tomography Radiomics, Clinical, and Tumor Immune Features for Analysis of Disease-Free Survival of NSCLC Patients with Surgery

To improve prognosis of cancer patients and determine the integrative value for analysis of disease-free survival prediction, a clinic investigation was performed involving with 146 non-small cell lung cancer (NSCLC) patients (83 men and 73 women; mean age: 60.24 years ± 8.637) with a history of surgery. Their computed tomography (CT) radiomics, clinical records, and tumor immune features were firstly obtained and analyzed in this study. Histology and immunohistochemistry were also performed to establish a multimodal nomogram through the fitting model and cross-validation. Finally, Z test and decision curve analysis (DCA) were performed to evaluate and compare the accuracy and difference of each model. In all, seven radiomics features were selected to construct the radiomics score model. The clinicopathological and immunological factors model, including T stage, N stage, microvascular invasion, smoking quantity, family history of cancer, and immunophenotyping. The C-index of the comprehensive nomogram model on the training set and test set was 0.8766 and 0.8426 respectively, which was better than that of the clinicopathological-radiomics model (Z test, P =0.041<0.05), radiomics model and clinicopathological model (Z test, P =0.013<0.05 and P =0.0097<0.05). Integrative nomogram based on computed tomography radiomics, clinical and immunophenotyping can be served as effective imaging biomarker to predict DFS of hepatocellular carcinoma after surgical resection.
Monitoring and diagnosis of Alzheimer's disease using noninvasive compressive sensing EEG

The majority of elderly with Alzheimer’s Disease (AD) receive care at home from caregivers. In contrast to standard tethered clinical settings, a wireless, real-time, body-area smartphone-based remote monitoring of electroencephalogram (EEG) can be extremely advantageous for home care of those patients. Such wearable tools pave the way to personalized medicine, for example giving the opportunity to control the progression of the disease and the effect of drugs. By applying Compressive Sensing (CS) techniques it is in principle possible to overcome the difficulty raised by smartphones spatial-temporal throughput rate bottleneck. Unfortunately, EEG and other physiological signals are often non-sparse. In this paper, it is instead shown that the EEG of AD patients becomes actually more compressible with the progression of the disease. EEG of Mild Cognitive Impaired (MCI) subjects is also showing clear tendency to enhanced compressibility. This feature favor the use of CS techniques and ultimately the use of telemonitoring with wearable sensors.
TOPCon shingle solar cells: Thermal laser separation and passivated edge technology

This work shows the first demonstration of thermal laser separation (TLS) and post‐metallization passivated edge technology (PET) applied to tunnel‐oxide passivated contact (TOPCon) shingle solar cells. The shingle solar cells with 26.46 mm × 158.75 mm size are separated from industrial full‐square TOPCon host solar cells. The singulation is performed either by TLS from the front side (emitter side) or by conventional laser scribe and mechanical cleaving (LSMC) from the rear side (emitter‐free side). The TLS optimized in this work yields up to 0.2%abs more efficient shingle cells after separation in comparison with LSMC‐separated shingle cells. The most promising PET sequence identified for the singulated TOPCon cells consists of depositing an 8‐nm‐thin aluminum oxide layer by thermal atomic layer deposition at a temperature below 200°C in conjunction with subsequent hotplate annealing at 250°C. Application of the PET yields a boost of up to 0.5%abs in energy conversion efficiency for edge‐passivated TOPCon shingle cells in comparison with their performance directly after separation. This efficiency‐increasing impact of the PET sequence is found not to be strongly dependent on the separation process applied. The most efficient TOPCon shingle cell after PET achieves an efficiency of 22.0% and has been singulated by TLS.
Design and Simulation of Eighth-Order Active-R Band Pass Filter using Multiple Feedback Topology

In this paper, and active Eight-order R band pass filter is designed and simulated at centre frequency f0 of 284KHz and quality factor Q of 30. The filter is designed from given specifications of the filter. The architecture that will be used is the multiple feedback and the gain magnitude response is simulated using MULTISIM work bench version 11.0. The simulation shows that the bandwidth of the filter at stage 3 was most selected with Bandwidth of 7.65KHz and the roll-off of the filter with values approaching -60dB/decade instead of an Eight-order which is -160dB/decade and there was a shift in the centre frequency of the filter, which is only a slight shift. The filter had a high mid-band gain that increased with each stage 1 (40.14dB) to stage 4 (87.50dB).
Multi-Component Systems and Structures and Their Reliability

A number of recent publications have dealt with problems of analyzing the performance of multi-component systems and evaluating their reliability. For example, a comprehensive theory of two-terminal networks was presented in [I] by Moore and Shannon who, among other results, have developed methods for obtaining highly reliable systems using components of low reliability; some of their procedures are credited to earlier work by von Neumann [2]. Several of the concepts and results of the present paper are generalizations of the corresponding concepts and results of the Moore-Shannon paper. A discussion of complex systems interpreted as Boolean functions may be found in the paper [3] by Mine. The present study deals with general classes of systems which contain two-terminal networks and most other kinds of systems considered previously as special cases, and investigates their combinatorial properties and their reliability. These classes consist, with several variants, of systems such that the more components.
Requirement for ER-mitochondria Ca2+ transfer, ROS production and mPTP formation in L-asparaginase-induced apoptosis of acute lymphoblastic leukemia cells

Acute lymphoblastic leukemia (aLL) is a malignant cancer in the blood and bone marrow characterized by rapid expansion of lymphoblasts. It is a common pediatric cancer and the principal basis of cancer death in children. Previously, we reported that L-asparaginase, a key component of acute lymphoblastic leukemia chemotherapy, causes IP3R-mediated ER Ca2+ release, which contributes to a fatal rise in [Ca2+]cyt, eliciting aLL cell apoptosis via upregulation of the Ca2+-regulated caspase pathway (Blood, 133, 2222–2232). However, the cellular events leading to the rise in [Ca2+]cyt following L-asparaginase-induced ER Ca2+ release remain obscure. Here, we show that in acute lymphoblastic leukemia cells, L-asparaginase causes mitochondrial permeability transition pore (mPTP) formation that is dependent on IP3R-mediated ER Ca2+ release. This is substantiated by the lack of L-asparaginase-induced ER Ca2+ release and loss of mitochondrial permeability transition pore formation in cells depleted of HAP1, a key component of the functional IP3R/HAP1/Htt ER Ca2+ channel. L-asparaginase induces ER Ca2+ transfer into mitochondria, which evokes an increase in reactive oxygen species (ROS) level. L-asparaginase-induced rise in mitochondrial Ca2+ and reactive oxygen species production cause mitochondrial permeability transition pore formation that then leads to an increase in [Ca2+]cyt. Such rise in [Ca2+]cyt is inhibited by Ruthenium red (RuR), an inhibitor of the mitochondrial calcium uniporter (MCU) that is required for mitochondrial Ca2+ uptake, and cyclosporine A (CsA), an mitochondrial permeability transition pore inhibitor. Blocking ER-mitochondria Ca2+ transfer, mitochondrial ROS production, and/or mitochondrial permeability transition pore formation inhibit L-asparaginase-induced apoptosis. Taken together, these findings fill in the gaps in our understanding of the Ca2+-mediated mechanisms behind L-asparaginase-induced apoptosis in acute lymphoblastic leukemia cells.
A numerical investigation of HVPSAW in LiTaO/sub 3/ with uniform gold film and periodic gold grating

The paper reports on the results of numerical investigation of high-velocity pseudo-surface waves (HVPSAW) in /spl theta/-rotated Y-cuts of lithium tantalate (LT), 90/spl deg/-propagation. Attenuation coefficients of HVPSAW are compared for LT with Au uniform film and with Au periodic grating. The results are presented as contour plots of attenuation versus film or electrode thickness and rotation angle. HVPSAW degenerates into a pure HVSAW when /spl theta/=29/spl deg/ and 127/spl deg/, with Au film thickness h//spl lambda/=4.8% and 5.0%, respectively. These optimal orientations move to /spl theta/=39/spl deg/ and /spl theta/=118/spl deg/, in a substrate with Au grating, with optimal electrode thickness reduced to h//spl lambda/=2.55%. In both orientations low-attenuated HVPSAWs exhibit fairly high piezoelectric coupling (2-6%). Analysis of strip admittance confirmed that HVPSAW resonator with high quality factor can be built on the found orientations. Velocity dispersion curve was calculated for the optimal cut with the grating of optimized thickness.
Alignment of LBT optics using a laser tracker

The laser tracker allows the precise determination of positions of surfaces in three dimensions over volumes exceeding 30 m radius from the tracker head. At the Large Binocular Telescope a laser tracker has recently been employed for the initial alignment of all telescope optics in the right hand side (DX) bent Gregorian optical train. In this paper the particular approach to alignment of optical elements employed during this campaign is discussed in detail, together with results and expected accuracies. Subsequent to this "mechanical alignment" the telescope was taken "onsky" and a subsequent "optical alignment" using a Shack-Hartman wavefront sensor with stellar sources took place. Ongoing activities include using the laser tracker to measure elevation and thermally induced displacements of individual optical elements.
The probability of misassociation between neighboring targets

This paper presents procedures to calculate the probability that the measurement originating from an extraneous target will be (mis)associated with a target of interest for the cases of Nearest Neighbor and Global association. It is shown that these misassociation probabilities depend, under certain assumptions, on a particular - covariance weighted - norm of the difference between the targets' predicted measurements. For the Nearest Neighbor association, the exact solution, obtained for the case of equal innovation covariances, is based on a noncentral chi-square distribution. An approximate solution is also presented for the case of unequal innovation covariances. For the Global case an approximation is presented for the case of "similar" innovation covariances. In the general case of unequal innovation covariances where this approximation fails, an exact method based on the inversion of the characteristic function is presented. The theoretical results, confirmed by Monte Carlo simulations, quantify the benefit of Global vs. Nearest Neighbor association. These results are applied to problems of single sensor as well as centralized fusion architecture multiple sensor tracking.
Effect of Surface Curvature on Heat Transfer and Hydrodynamics Within a Single Hemispherical Dimple

Turbulent heat transfer and hydrodynamics have been studied in concavely and convexly curved dimples with Reynolds numbers ranging from 1.3×10 5 to 3.1 × 10 5 . The large-scale single hemispherical dimple 50 mm in diameter and 25 mm in depth was arranged on the smooth concave or convex wall of a curved rectangular-shaped passage. The fluid flow and heat transfer measurements, and surface streamline observations were performed within the flow curvature parameter δ ** /R ranged from 0.002 to 0.007. The tornado-like oscillating vortex bursting periodically out of the dimple was registered in the experiments with a curved dimple. This vortex structure is similar to that earlier observed in a flat dimple. The surface curvature considerably influences the dimple heat transfer rate in both cases. It enhances heat transfer in a concave dimple and reduces it in a convex one; however, the more remarkable effect occurred in a concavely curved dimple. The correction factors describing the effect of curvature on average heat transfer in a curved dimple have been obtained as a result of experimental study.
An Application of the Maximum Entropy Production Method in the WRF Noah Land Surface Model

The calculation of surface sensible heat (SH) and latent heat (LE) fluxes using the bulk transfer models for complex terrains, tall vegetation regions, and morning and evening transition periods remains a challenging problem in numerical weather and climate models. The maximum entropy production (MEP) model, a new method of calculating surface heat fluxes, is coupled with the Noah land surface model (LSM) in the Weather Research and Forecasting (WRF) model. Surface heat fluxes and meteorological variables including air temperature, relative humidity, soil temperature, and precipitation data at nine intensive observation stations and 453 routine meteorological operational observation stations in the Tibetan Plateau (TP) from 1 June to 31 August 2015 are used to evaluate the coupled model. The results show that the MEP method improves the nonclosure of the surface energy balance in the WRF (with an energy residual from 24.3 to 1.9 W m−2), reducing the overestimations of SH and LE and the underestimation of the surface ground heat flux over the complex terrain of TP (with the bias decreases of 50.6% and 117.1% for SH and LE in turn), especially during the daytime. The improved SH and LE reduce the cold and wet biases in the TP by 29.4% and 51.7%, respectively. The MEP model also reduces the overestimated soil temperature by 63%. Moreover, the overestimated daily precipitation is reduced by 3% over the TP. The successful application of the MEP method demonstrates its advantage over the bulk transfer method, providing a new approach for reducing the overestimation of surface heat fluxes and wet and cold biases in numerical forecast models in complex terrains.
Towards automated data collection at the Stanford Synchrotron Radiation Laboratory

The Stanford Synchrotron Radiation Laboratory (SSRL) will experience a significant increase in the brightness of its X-ray beamlines once the SPEAR3 storage ring upgrade is complete in early 2004 [1]. On the protein crystallography beamlines, a high-quality diffraction image will be collected in just seconds, and data sets will be completed in a matter of minutes when the accelerator is run at its final design current. With such powerful experimental stations, the need for fully automated sample changing is essential in order to make efficient use of the facility. In addition, a high-speed crystal screening system will allow users to widen the scope of their experimental goals. The Structure Determination Core of the Joint Center for Structural Genomics (http://www.jcsg.org) and the Structural Molecular Biology group (http://smb.slac.stanford.edu) at SSRL have developed an automated crystal screening environment and data collection facility on beamline 11-1, where it has been operational for approximately 12 months. At the start of the SPEAR3 run, the system will be available to the general user community on four beam lines. The system employs a robotic sample changer and an automated crystal centering algorithm to complete the crystal screening process without human intervention. In order to facilitate the synchronization of experimental information between the home laboratory and the experimental facility, a web interface has been developed that allows users to upload crystal information using an Excel spreadsheet. The automation systems will be installed on two additional beam lines, and thus is soon a standard component on all macromolecular crystallography beam lines at SSRL.