Miracle-dz/UniFAR-FTUs huggingface dataset:
Quantifying the decline in transpiration of Tsuga Canadensis and predicting water budget implications of succession in southern Appalachian forests.

Eastern hemlock (Tsuga canadensis (L.) Carr. ) is declining throughout the eastern United States as a result of infestation of the hemlock woolly adelgid (HWA). As a principal species in riparian cove habitats in the southern Appalachians, its loss will have impacts on the hydrologic budget in these systems. To estimate the impact on the hydrologic budget, we quantified transpiration over five years for T. canadensis, and over two years for co-occurring species Acer rubrum, Betula lenta, and Rhododendron maximum. Further, to understand the impacts of climate on transpiration, we compared transpiration to photosynthetically active radiation (PAR) and to vapor pressure deficit (VPD). Given the loss of T. canadensis from the ecosystem, we modeled implications on transpiration from two resulting succession scenarios, one in which R. maximum dominates, and one in which A. rubrum and B. lenta dominate. Transpiration was shown to decline since 2004 for T. canadensis, and no such decline was observed for the other species from 2006. The decline in transpiration was not shown to be a result of a changing climate conditions from the same study period. Using data from other studies, we modeled the succession of R. maximum following the loss of T. canadensis leaf area from the canopy. Also, we modeled the succession of A. rubrum and B. lenta resulting from a shift in sapwood area from T. canadensis to these species. Under both post-mortality scenarios, the transpiration component of the hydrologic budget increased. Although actual post-mortality scenarios are difficult to predict, the loss of T. canadensis will result in changes in the function of this ecosystem. Key -words: hydrologic budget; sap flux density; sap-wood; succession; Tsuga canadensis.
Research on the Application of Speech Database based on Emotional Feature Extraction in International Chinese Education and Teaching

The advanced analysis of the relationship between acoustic and emotional characteristics of speech signals can effectively improve the interactivity and intelligence of computers. Given the current status of speech recognition and the problems encountered in international Chinese education, the study proposes to extract emotional characteristics to achieve speech construction of the database. Based on considering the emotional characteristics of speech, a hybrid algorithm based on spectral sequence context features is proposed. The DBN-BP algorithm is used to process emotional data of different dimensions, and a speech database is constructed. After testing and analyzing the algorithm model, it is found that the dynamic recognition accuracy of the DBN-BP model fused with emotional features is over 90%, and the negative emotion recognition rates in the three databases are all above 60%. At the same time, the accuracy rate of the model in the algorithm comparison experiment remains above 85%, the data information extraction is relatively complete, and the average test time of less than 1s is less than 3%. The speech database based on multi-emotional feature extraction can effectively provide a new reference for the improvement of the quality of Chinese international education and the improvement of the speech recognition system.
A pan-cancer analysis of the prognostic implication and oncogenic role of tubulin epsilon and delta complex 2 (TEDC2) in human tumors

Introduction Tubulin epsilon and delta complex 2 (TEDC2) is widely expressed in various human tissues and primarily governs centriole stability. However, the biological significance of TEDC2 in pan-cancer is unclear. Methods In this study, we employed R software and various online bioinformatics analysis tools to investigate the functional attributes of TEDC2 in human tumours and its potential involvement in immune response. The status of TEDC2 expression was evaluated in samples from the TCGA and GEO datasets, as well as in tumour and corresponding normal samples from the TCGA database. Subsequently, Kaplan-Meier estimates, clinical correlations, and univariate Cox regressions were used to analyze the 33 types of tumors from TCGA and determine the prognostic significance of TEDC2. Moreover, nomogram models were formulated using three distinct tumours, namely kidney renal clear cell carcinoma (KIRC), lung adenocarcinoma (LUAD), and liver hepatocellular carcinoma (LIHC), to evaluate the prognostic significance of TEDC2 in tumours. Furthermore, TEDC2 was investigated for its correlation with the levels of immune cell infiltration, and a functional enrichment analysis was conducted to identify potential signalling pathways involving TEDC2. Results Differential analysis revealed that 16 tumour types expressed TEDC2 to a greater extent than normal tissues. The abnormal expression of TEDC2 can predict survival outcomes in patients with adrenocortical carcinoma (ACC), KIRC, kidney renal papillary cell carcinoma (KIRP), LUAD, LIHC, lower grade glioma (LGG), and thymoma (THYM). Subsequent results indicated that TEDC2 has the ability to influence ECM regulators, cell cycle, and Immune checkpoint-associated signalling pathways, which could potentially lead to a poor prognosis and tumour progression. Discussion TEDC2 has been identified as a potential therapeutic target that could predict the prognosis of multiple tumour types, making it a promising target for reversing tumour development.
Tourist Attraction Customer Satisfaction Index Model

Based on the improvement of current international major customer satisfactionindex models, and taking the characteristics of tourist attraction into consideration, this paperputs forward a creative model of Tourism Attraction Customer Satisfaction Index (TACSI). Itis a structural equation model with casual relationships, which is made up of 7 latent variablesand 23 observed variables. There are 11 kinds of positive and negative correlativities amongthe 7 latent variables. Trunk Hill Park in Guilin is taken as an example in empirical study. Bymeans of LISREL, the test result on TACSI model indicates that the measurement model hashigh goal dependability since the observed variables have a significant effect on customersatisfaction; the path coefficients between the latent variables are basically consistent with theassumption; and the whole TACSI model has a good goodness of fit. In addition, the authorscompare the fitness result of TACSI model with ACSI model, which shows that TACSI modelis more feasible because it can explain the variables better than ACSI model.
Large area nanoscale patterning of functional materials using organosilicate ink based nanotransfer printing

This paper presents a versatile nanotransfer printing method for achieving large-area sub-micron patterns of functional materials. Organosilicate ink formulations combined with effective release layers have been shown to facilitate patterning of materials through the commonly used patterning approaches—lift off, physical etching and chemical etching. In this paper, we demonstrate that organosilicate ink formulations function as an effective resist owing to its superior physico-chemical stability whereas the release layers ensure clean removal of the resist post patterning. We successfully demonstrate patterning of sub-micron structures (800 nm feature sizes) of chromium metal through the lift off approach, silicon through reactive ion etching technique and silicon dioxide through wet chemical etching technique illustrating the versatility of the reported method. This patterning methodology represents a significant advancement in enabling nanostructure fabrication within resource-constrained laboratories. The approach requires nothing more than a master mold containing the desired structures, a spin coater, a low-temperature hotplate, and a desktop reactive ion etch tool.
Physiological Responses to Supramaximal Running Exercise with End-Expiratory Breath Holding up to the Breaking Point

This study aimed to assess the physiological responses to repeated running exercise performed at supramaximal intensity and with end-expiratory breath holding (EEBH) up to the breaking point. Eight male runners participated in two running testing sessions on a motorized treadmill. In the first session, participants performed two sets of 8 repetitions at 125% of maximal aerobic velocity and with maximum EEBH. Each repetition started at the onset of EEBH and ended at its release. In the second session, participants replicated the same procedure, but with unrestricted breathing (URB). The change in cerebral and muscle oxygenation (Δ[Hbdiff]), total haemoglobin concentration (Δ[THb]) and muscle reoxygenation were continuously assessed. End-tidal oxygen (PETO2) and carbon dioxide pressure (PETCO2), arterial oxygen saturation (SpO2) and heart rate (HR) were also measured throughout exercise.On average, EEBH was maintained for 10.1 ± 1 s. At the breaking point of EEBH, PETO2 decreased to 54.1 ± 8 mmHg, whereas PETCO2 increased to 74.8 ± 3.1 mmHg. At the end of repetitions, SpO2 (nadir values 74.9 ± 5.0 vs. 95.7 ± 0.8%) and HR were lower with EEBH than with URB. Cerebral and muscle Δ[Hbdiff] were also lower with EEBH, whereas this condition induced higher cerebral and muscle Δ[THb] and greater muscle reoxygenation. This study showed that performing repeated bouts of supramaximal running exercises with EEBH up to the breaking point induced a fall in arterial, cerebral and muscle oxygenation compared with the URB condition. These phenomena were accompanied by increases in regional blood volume likely resulting from compensatory vasodilation to preserve oxygen delivery to the brain and muscles.
Use of PhET Simulations as A Virtual Laboratory to Improve Students' Problem Solving Skills

In the Radiochemistry course so far there have been no experimental activities because there are no laboratory facilities. However, with the use of technology, students can now easily access a wealth of educational resources online. This research aims to determine the effect of using the PhET Simulations as a virtual laboratory on students' problem solving skills. This research used mixed methods that combines quantitative and qualitative research. A quasi-experiment was carried out using a one group pretest-posttest design then continued with in-depth interviews. The instruments used in this research consisted of written tests to collect data on problem solving skills, questionnaires to collect student response, and interview guides to collect data on the problem solving process. Data in the form of problem solving skills were analyzed statistically using the SPSS 22.0 program while data obtained through questionnaires and interviews were analyzed descriptively. The results of the hypothesis test using Wilcoxon signed rank test show a significant value of 0.001, which means that PhET simulations as a virtual laboratory improve students’ problem solving skills. Students are divided into high-medium-low groups based on problem solving ability and the majority of students are in the medium-ability group. Students responded positively to PhET Simulations as a virtual laboratory. In addition, interview transcripts provide an overview of how students think when solving chemistry problems.
Age at first birth, age at menopause, and risk of ovarian cyst: a two-sample Mendelian randomization study

Background Increasing observational studies have indicated that hormonal reproductive factors were associated with ovarian cyst, a common gynecological disease. A two-sample Mendelian randomization (MR) was carried out by investigating the causality of reproductive factors including age at first birth (AFB), age at natural menopause (ANM), and age at menarche (AAM), and the risk of ovarian cyst (OC). Method Summary statistics were collected from a large genome-wide association study (GWAS), and we used a two-sample MR study to clarify the causal association between the exposure of AFB (N = 542,901), ANM (N = 69,360), and AAM (N = 29,346) and the outcome of the OC (N case = 20,750, N control = 107,564). We separately selected 51, 35, and 6 single-nucleotide polymorphisms (SNPs) as instrumental variables (IVs) for assaying the influence of AFB, ANM, and AAM on OC, respectively. Then, the causal relationship was tested through multiple approaches including an inverse-variance weighted method, an MR-Egger regression, and a weighted median method. In addition, the MR-PRESSO method was also used to verify the horizontal pleiotropy. Subsequently, we adjust the confounders for MR design. Results The MR analysis results showed that AFB was negatively associated with the OC (IVW Beta: −0.09, OR: 0.91, 95% CI: 0.86–0.96, p = 0.00185), and the greater AAM decreased the risk of OC (IVW Beta: −0.10, OR: 0.91, 95% CI: 0.82–0.99, p = 0.0376). However, ANM has a positive correlation with the OC (IVW Beta: 0.05, OR: 1.05, 95% CI: 1.03–1.08, p = 8.38 × 10−6). After adjusting BMI, alcohol intake frequency, and ever smoked, we also obtained a negative relationship between AFB and OC (p < 0.005). Meanwhile, we adjusted weight, alcohol intake frequency, and height, and then found a causal relationship between older AMN and an increased risk of OC (p < 0.005). Conclusion A causal effect of reproductive factors on the development of OC, affected by AFB, ANM, and AAM, was found convincingly. After adjusting the confounders, we also successfully found the substantial causal effect of younger AFB, younger AAM, and older ANM on an increased risk of OC.
Effects of a Singular Dose of Mangiferin–Quercetin Supplementation on Basketball Performance: A Double-Blind Crossover Study of High-Level Male Players

Pre-exercise mangiferin–quercetin may enhance athletic performance. This study investigated the effect of mangiferin–quercetin supplementation on high-level male basketball players during a basketball exercise simulation test (BEST) comprising 24 circuits of 30 s activities with various movement distances. The participants were divided into two groups (EXP = 19 and CON = 19) and given a placebo one hour before the BEST (PRE-condition). The following week, the EXP group received mangiferin–quercetin (84 mg/140 mg), while the CON group received a placebo (POST-condition) before the BEST in a double-blind, cross-over design. The mean heart rate (HR) and circuit and sprint times (CT and ST) during the BEST were measured, along with the capillary blood lactate levels (La−), the subjective rating of muscle soreness (RPMS), and the perceived exertion (RPE) during a resting state prior to and following the BEST. The results showed significant interactions for the mean CT (p = 0.013) and RPE (p = 0.004); a marginal interaction for La− (p = 0.054); and non-significant interactions for the mean HR, mean ST, and RPMS. Moreover, the EXP group had significantly lower values in the POST condition for the mean CT (18.17 ± 2.08 s) and RPE (12.42 ± 1.02) compared to the PRE condition (20.33 ± 1.96 s and 13.47 ± 1.22, respectively) and the POST condition of the CON group (20.31 ± 2.10 s and 13.32 ± 1.16, respectively) (p < 0.05). These findings highlight the potential of pre-game mangiferin–quercetin supplementation to enhance intermittent high-intensity efforts in sports such as basketball.
Nanostructure Lipid Carrier of Curcumin Co-Delivered with Linalool and Geraniol Monoterpenes as Acetylcholinesterase Inhibitor of Culex pipiens

(1) Background: A molecular hybridization docking approach was employed to develop and detect a new category of naturally activated compounds against Culex pipiens as acetylcholinesterase inhibitors via designing a one-pot multicomponent nano-delivery system. (2) Methods: A nanostructure lipid carrier (NLC), as a second generation of solid lipid nanoparticles, was used as a carrier to deliver the active components of curcumin (Cur), geraniol (G), and linalool (L) in one nanoformulation after studying their applicability in replacing the co-crystallized ligand imidacloprid. (3) Results: The prepared nanostructure showed spherical-shaped, polydisperse particles ranging in size from 50 nm to 300 nm, as found using a transmission electron microscope. Additionally, dynamic light scattering confirmed an average size of 169 nm and a highly stable dispersed solution, as indicated by the zeta potential (−38 mV). The prepared NLC-Cur-LG displayed competitive, high-malignancy insecticidal activity against fourth instar C. pipiens with an elevated rate of death of 0.649 µg/mL. The treatment, due to the prepared nanostructure, affects oxidative stress enzymes, e.g., hydrogen peroxide (4 ppm), superoxide dismutase (SOD) (0.03 OD/mg), and protein carbonyl (0.08 OD/mg), and there are observable upward and downward fluctuations when using different concentrations of NLC-Cur-LG, suggesting significant problems in its foreseeable insecticidal activity. The acetylcholinesterase activity was assessed by an enzyme inhibition assay, and strengthened inhibition occurred due to the encapsulated NLCs (IC50 = 1.95 µg/mL). An investigation of the gene expression by Western blotting, due to treatment with NLC-Cur-LG, revealed a severe reduction of nearly a quarter of what was seen in the untreated group. As a preliminary safety step, the nanoformulation’s toxicity against normal cell lines was tested, and a reassuring result was obtained of IC50 = 158.1 µg/mL for the normal lung fibroblast cell line. (4) Conclusions: the synthesized nanoformulation, NLC-Cur-LG, is a useful insecticide in field conditions.
Whole Genome Sequence for Klebsiella pneumoniae isolate from Burn Skin

Klebsiella pneumoniae is a type of gram-negative bacterium that was initially discovered and isolated by Carl Friedlander in the year 1882. Klebsiella pneumoniae is responsible for a range of nosocomial and community-acquired illnesses, including urinary tract infections (UTIs), pneumonia, surgical site infections, and bloodstream infections. According to a number of studies, gram-negative sepsis ranks as the second most prevalent cause. A comprehensive analysis of the entire genome of Klebsiella pneumoniae was conducted using Illumina sequencing by synthesis (SBS) technology, specifically the Illumina HiSeq platform. The genetic material of the organism was comprised of a single circular chromosome of three thousand base pairs in length, exhibiting a GC content of 55.90%. In total, the genome exhibited a collective count of 164 genes associated with transfer RNA, 9 genes associated with ribosomal RNA, and 11,132 protein-coding sequences. Moreover, the analysis of genetic diversity entailed the application of sequence data, which was then compared to the reference genome that shared the highest degree of similarity. The genome annotation service offered by PATRIC and the RAST annotation system have successfully detected many antimicrobial resistance elements, clusters of genes associated with antibiotic resistance, as well as efflux pumps. This study represents the first known case of conducting a comprehensive genome sequencing of Klebsiella pneumoniae within the geographic area of Iraq, based on our current knowledge. The current investigation holds potential for advancing our comprehension of the antibiotic resistance traits demonstrated by Klebsiella pneumoniae, therefore enabling the healthcare facility to efficiently handle and alleviate outbreaks.
Practical exploitation of mycorrhizal fungi in agricultural systems.

Improving the sustainability of agricultural systems requires a more efficacious use of soil resources. Mycorrhizas are known to contribute to host plant P acquisition and protection against both biotic and abiotic stresses, such as soil-borne diseases and toxic metal ions. However, practical exploitation of the mutualistic relationship is rarely considered in agricultural systems, allegedly owing to the cost of inoculation and the requirement for timely colonisation. To overcome these limitations, the presence of an extensive extraradical mycelium (ERM) from indigenous arbuscular mycorrhizal fungi (AMF) could be used as the preferential source for colonisation of a crop plant. Colonisation of crop roots starting from an intact ERM takes place faster and generally forms a more effective mycorrhizal association than when initiated from other propagules such as spores and root fragments. We report on the ability of an intact ERM developed by indigenous AMF population on mycotrophic plants (Developers) to significantly improve the AMF colonisation of wheat, subterranean clover and maize allowing for a better performance of the crop. This mechanism allowed the protection of wheat and subterranean clover from excessive Mn concentration in the shoots or in the roots, as in the case of the clover, leading to a greater growth of the crop (2.7 and 4.7 times respectively). Using the same strategy to promote the AMF colonisation of maize, the crop was able to exhibit high levels of colonization, even up to 45 kg P.ha-1 of applied P, and the plants took advantage in terms of P use efficiency. Our results indicate that the use of intact ERM as preferential AMF propagule is a valid strategy to increase the role of this symbiosis under marginal or more intensive cropping systems, through simple adaptations to both crop rotations and tillage practices.
Authentication Model for Location based Queries

The popularity of location-based services leads to serious concerns on user privacy. It is very easy for a person to know his/her location with the help of devices having GPS facility. When user’s location is provided to Location Based Services (LBS), it is possible for user to know all location dependent information like location of friends or Nearest Restaurant, weather or traffic conditions. The massive use of mobile devices paves the way for the creation of wireless networks that can be used to exchange information. When the exchange of information is done amongst entrusted parties, the privacy of the user could be in harmful. Existing protocol doesn’t work on many different mobile devices and another issue is that, Location Server (LS) should provide misleading data to user. This gives rise to new challenges and reconsideration of the existing privacy metrics.
Assessment of a Flow-Measurement Technique for the Printability of Extrusion-Based Bioprinting

Extrusion-based 3D bioprinting is a common application in tissue engineering used to fabricate porous and complex scaffolds for tissue regeneration and repair. Flow-rate measurement can improve printability and reduce the trial-and-error steps needed to optimize printing parameters. This study develops a novel system for flow-rate measurement of chitosan solution based on particle image velocimetry (PIV). The results obtained using PIV are compared with the results obtained using an existing predictive model. In addition, results are double-checked using precision scales to demonstrate the robustness of the PIV method. The experimental results fit the exponential function of the mathematical model (adjusted R-squared = 0.999), and the measured errors of PIV compared to those of the precision scale were within 3%.