Miracle-dz/UniFAR-FTUs huggingface dataset:
Nasal and Exhaled Nitric Oxide in Allergic Rhinitis

Objectives The primary aim of this study was to assess whether one can use levels of nasal nitric oxide (nNO) and exhaled nitric oxide (eNO) as a means of evaluation in allergic rhinitis. Methods We used a chemiluminescence analyzer to measure nNO and eNO in normal controls (n=34) and allergic rhinitis patients (n=35), and compared these measurements with various parameters of clinical symptoms and laboratory data. Results Mean nNO (389±119 ppb) in allergic rhinitis patients was significantly higher than normal controls (276±88 ppb). Without asthma, mean eNO (64.8±55.9 ppb) in allergic rhinitis patients was significantly higher than normal controls (33.0±24.0 ppb). In the persistent allergic rhinitis group, eNO concentration was significantly higher, while nNO concentration was significantly lower than the intermittent group. Conclusion We can use nNO and eNO levels for evaluation of allergic rhinitis. However, we should consider the fact that nNO levels can be reduced, when symptoms are severe and long-lasting. Additionally, in allergic rhinitis, eNO can be elevated without asthma.
Social Network Modulation of Reward-Related Signals

Everyday goals and experiences are often shared with others who may hold different places within our social networks. We investigated whether the experience of sharing a reward differs with respect to social network. Twenty human participants played a card guessing game for shared monetary outcomes with three partners: a computer, a confederate (out of network), and a friend (in network). Participants subjectively rated the experience of sharing a reward more positively with their friends than the other partners. Neuroimaging results support participants' subjective reports, as ventral striatal BOLD responses were more robust when sharing monetary gains with a friend as compared to the confederate or computer, suggesting a higher value for sharing with an in-network partner. Interestingly, ratings of social closeness covaried with this activity, resulting in a significant partner × closeness interaction; exploratory analysis showed that only participants reporting higher levels of closeness demonstrated partner-related differences in striatal BOLD response. These results suggest that reward valuation in social contexts is sensitive to distinctions of social network, such that sharing positive experiences with in-network others may carry higher value.
Evaluation of digital codings on the SoC-based Software-Defined radio for the military communication

The modern military communication extensively utilizes radio spectrums to convey important information. Radio transceivers have evolved from large analog transceivers to miniature digital transceivers. Digital transceivers are relied on digital communication techniques, which include various digital codings and digital modulations. Digital codings are crucial processes in most digital communications. The outstanding advantages of digital codings are that it conserves channel bandwidth, and it makes information more robust. There are plenty of digital codings in military communications. Each coding is suitable for the specific application and environment. The implementation of various digital codings on one device is possible with a software defined radio (SDR), at which the transmission characteristics of a SDR can be defined by the software running on the device. Hence, a SDR can be reprogrammed to meet the require transmission characteristics. A logic parts of SDR can be implemented either by Digital Signal Processor (DSP) or Field Programmable Gate Array (FPGA). With the current semiconductor technology, both DSP and FPGA can be integrated into one chip, which is known as a System-on-Chip (SoC). This article evaluates the performance of the military digital codings on SoC as the digital processing unit. The result shows that SoC-based SDR can be a potential for SDR in the military communications.
Appropriate Time Interval to Repeat Ambulatory Blood Pressure Monitoring in Patients With White-Coat Resistant Hypertension

Resistant hypertension is defined as uncontrolled office blood pressure, despite the use of ≥3 antihypertensive drugs. Ambulatory blood pressure monitoring (ABPM) is mandatory to diagnose 2 different groups, those with true and white-coat resistant hypertension. Patients are found to change categories between controlled/uncontrolled ambulatory pressures without changing their office blood pressures. In this way, ABPM should be periodically repeated. The aim of this study was to evaluate the most appropriate time interval to repeat ABPM to assure sustained blood pressure control in patients with white-coat resistant hypertension. This prospective study enrolled 198 patients (69% women; mean age: 68.9±9.9 years) diagnosed as white-coat resistant hypertension on ABPM. Patients were submitted to a second confirmatory examination 3 months later and repeated twice at 6-month intervals. Statistical analyses included Bland-Altman repeatability coefficients and multivariate logistic regression. Mean office blood pressure was 163±20/84±17 mm Hg, and mean 24-hour blood pressure was 118±8/66±7 mm Hg. White-coat resistant hypertension diagnosis presented a moderate reproducibility and was confirmed in 144 patients after 3 months. In the third and fourth ABPMs, 74% and 79% of patients sustained the diagnosis. In multivariate regression, a daytime systolic blood pressure ⩽115 mm Hg in the confirmatory ABPM triplicated the chance of white-coat resistant hypertension status persistence after 1 year. In conclusion, a confirmatory ABPM is necessary after 3 months of the first white-coat–resistant hypertension diagnosis, and the procedure should be repeated at 6-month intervals, except in patients with daytime systolic blood pressure ⩽115 mm Hg, in whom it may be repeated annually.
Genetic Immunization of Wild-Type and Hepatitis C Virus Transgenic Mice Reveals a Hierarchy of Cellular Immune Response and Tolerance Induction against Hepatitis C Virus Structural Proteins

ABSTRACT To study the effect of genetic immunization on transgenic expression of hepatitis C virus (HCV) proteins, we evaluated the immunological response of HCV transgenic mice to HCV expression plasmids. FVB/n transgenic mice expressing HCV structural proteins (core, E1, and E2) and wild-type (WT) FVB/n mice were immunized intramuscularly with plasmids expressing core (pHCVcore) or core/E1/E2 (pHCVSt). After immunization, HCV-specific humoral and cellular immune response was studied. Both WT and transgenic mice immunized with either HCV construct produced antibodies and exhibited T-cell proliferative responses against core or envelope. In WT mice immunized with pHCVSt, cytotoxic T-lymphocyte (CTL) activities were detected against E2 but not against core or E1, whereas strong CTL activities against core could be detected in WT mice immunized with pHCVcore. In pHCVSt-immunized, transgenic mice, CTL activities against the core or envelope were completely absent, but core-specific CTL activities could be detected in pHCVcore-immunized transgenic mice. A similar pattern of immune responses was also observed in other mouse strains, including a transgenic line expressing human HLA-A2.1 molecules (AAD mice). Despite the presence of a peripheral cellular immunity against HCV, no liver pathology or lymphocytic infiltrate was observed in these transgenic mice. Our study suggests a hierarchy of CTL response against the HCV structural proteins (E2 > core > E1) in vivo when the proteins are expressed as a polyprotein. The HCV transgenic mice can be induced by DNA immunization to generate anti-HCV antibodies and anticore CTLs. However, they are tolerant at the CTL level against the E2 protein despite DNA immunization.
Reactive Virtual Human with Bottom-up and Top-down Visual Attention for Gaze Generation in Realtime Interactions

We create a framework for physical interaction with virtual human. The virtual human reacts to the input of the user in realtime. The virtual human has an ocular system model, which mimics human ocular system, to generate natural and human-like gaze motions and to create inputs for the cognitive model of the virtual human. The visual input for virtual human is generated from the dynamics simulator for the virtual world. The gaze target is chosen by vying of bottom-up and top-down attentions. The motion controller for the eyes and head is based on observations and models of human gaze motion. We create a virtual boxing game as an application of proposed framework. Proposed framework succeeds to create natural gaze reaction. In addition, by changing the balance of the bottom-up and top-down attention, looks of concentration of the virtual human can be tuned.
The Interaction of Peripheral Proteins and Membranes Studied with α-Lactalbumin and Phospholipid Bilayers of Various Compositions*

To characterize the interaction of peripheral proteins and membranes at the molecular level, we studied the reversible association of bovine α-lactalbumin (BLA) with lipid bilayers composed of different molecular forms of phosphatidylserine or equimolar mixtures of these phosphatidylserine forms and egg yolk phosphatidylcholine. At pH 4.5, almost all BLA (>90%) associates to negatively charged small unilamellar vesicles. The conformational changes that binding to these bilayers induced on the protein were characterized by circular dichroism and fluorescence spectroscopy. Because binding of BLA to negatively charged vesicles is reverted by adjusting the pH back to >6.0, we also investigated the conformation of the membrane-bound protein by NMR-monitored H-D exchange of the backbone amide protons. The conformation adopted by BLA bound to these bilayers resembles a molten globule-like state but the negative ellipticity at 222 nm and the apparent α-helix content of the bound protein senses the changes in the physical properties of the membrane. Binding to bilayers in the gel state appears to correlate with an increased amount of α-helical structure and with a lower extent of integration into the membrane, corresponding to the adsorbed protein, while the opposite is found for BLA bound to vesicles in the liquid-crystalline phase, corresponding to the embedded conformation. A common feature for the membrane-bound conformations of BLA is that the amphipathic helix C (residues 86 to 99) is an important determinant for the adsorption and further integration of the protein into the membrane.
∞ Queues with Renewal Alternating Interruptions

We study G/G/∞ queues with renewal alternating service interruptions, where the service station experiences up and down periods. The system operates normally in the up periods, and all servers stop functioning while customers continue entering the system during the down periods. The amount of service a customer has received when an interruption occurs will be conserved and the service will resume when the down period ends. We use a two-parameter process to describe the system dynamics: X(t, y) tracking the number of customers in the system at time t that have residual service times strictly greater than y. The service times are assumed to satisfy either of the two conditions: (i) i.i. d. with a distribution of a finite support, or (ii) a stationary and weakly dependent sequence satisfying the φ-mixing condition and having a continuous marginal distribution function. We consider the system in a heavytraffic asymptotic regime where the arrival rate gets large and service time distribution is fixed, and the interruption down times are asymptotically negligible while the up times are of the same order as the service times. We show FLLN and FCLT for the process X(t, y) in this regime, where the convergence is in the space D([0,∞), (D, L1)) endowed with the Skorohod M1 topology. The limit processes in the FCLT possess a stochastic decomposition property. K eywords: G/G/∞ queue; dependent service times; service interruptions; two-parameter stochastic processes; FLLN; FCLT; Skorohod M1 topology
Ascending, unobstructed urinary tract infection in mice caused by pyelonephritogenic Escherichia coli of human origin

A model for ascending unobstructed urinary tract infection was developed in mice to study the pathogenesis of urinary tract infection induced by Escherichia coli associated with urinary tract infection in humans. Specifically, the model was designed to monitor the initial stages of the infectious process, e.g., bacterial adhesion. Mice were selected since the specificity and intensity of bacterial attachment of pyelonephritogenic E. coli strains to human and mouse uroepithelial cells were similar. Female mice were infected by urethral catheterization and installation of bacteria in the urinary bladder. To maximize clearance of unattached bacteria, no obstructive manipulations were performed. After sacrifice, the persistence of bacteria in kidneys and bladder was determined by viable counts on homogenized tissues. The experimental infection was standardized by using one pyelonephritis (HU734) and one normal fecal (414) E. coli isolate. With both strains all of the bladders became infected, but E. coli 414 was eliminated more rapidly than HU734. The percentage of positive kidney cultures increased with the bacterial inoculum concentration and volume. An inoculum of 0.05 ml containing 10(10) bacteria per ml was selected, giving the highest percentage of positive kidney cultures without detectable bacterial spread to the blood stream. The variation in the percentage of positive kidney cultures possibly depended on the degree of vesicoureteric reflux in the individual animals. Both in the kidneys and in the urinary bladders, strain HU734 yielded higher numbers of bacteria at 24 h and persisted longer than did strain 414. Several E. coli pyelonephritis isolates with properties associated with virulence in the human urinary tract consistently were recovered from mouse kidneys and bladders in higher numbers than E. coli strains of human fecal origin lacking those properties. The role of bacterial adhesion per se is the topic of the accompanying paper.
A comparison of adaptive sampling designs and binary spatial models : a simulation study using a census of Bromus inermis

Commonly in environmental and ecological studies, species distribution data are recorded as presence or absence throughout a spatial domain of interest. Field based studies typically collect observations by sampling a subset of the spatial domain. We consider the effects of six different adaptive and two non‐adaptive sampling designs and choice of three binary models on both predictions to unsampled locations and parameter estimation of the regression coefficients (species–environment relationships). Our simulation study is unique compared to others to date in that we virtually sample a true known spatial distribution of a nonindigenous plant species, Bromus inermis. The census of B. inermis provides a good example of a species distribution that is both sparsely (1.9 % prevalence) and patchily distributed. We find that modeling the spatial correlation using a random effect with an intrinsic Gaussian conditionally autoregressive prior distribution was equivalent or superior to Bayesian autologistic regression in terms of predicting to un‐sampled areas when strip adaptive cluster sampling was used to survey B. inermis. However, inferences about the relationships between B. inermis presence and environmental predictors differed between the two spatial binary models. The strip adaptive cluster designs we investigate provided a significant advantage in terms of Markov chain Monte Carlo chain convergence when trying to model a sparsely distributed species across a large area. In general, there was little difference in the choice of neighborhood, although the adaptive king was preferred when transects were randomly placed throughout the spatial domain. Copyright © 2013 John Wiley & Sons, Ltd.
Biosynthesis and Molecular Genetics of Polyketides in Marine Dinoflagellates

Marine dinoflagellates are the single most important group of algae that produce toxins, which have a global impact on human activities. The toxins are chemically diverse, and include macrolides, cyclic polyethers, spirolides and purine alkaloids. Whereas there is a multitude of studies describing the pharmacology of these toxins, there is limited or no knowledge regarding the biochemistry and molecular genetics involved in their biosynthesis. Recently, however, exciting advances have been made. Expressed sequence tag sequencing studies have revealed important insights into the transcriptomes of dinoflagellates, whereas other studies have implicated polyketide synthase genes in the biosynthesis of cyclic polyether toxins, and the molecular genetic basis for the biosynthesis of paralytic shellfish toxins has been elucidated in cyanobacteria. This review summarises the recent progress that has been made regarding the unusual genomes of dinoflagellates, the biosynthesis and molecular genetics of dinoflagellate toxins. In addition, the evolution of these metabolic pathways will be discussed, and an outlook for future research and possible applications is provided.
Genome-Wide Survey and Expression Analysis of the Putative Non-Specific Lipid Transfer Proteins in Brassica rapa L

Background Plant non-specific lipid transfer proteins (nsLtps) are small, basic proteins encoded by multigene families and have reported functions in many physiological processes such as mediating phospholipid transfer, defense reactions against phytopathogens, the adaptation of plants to various environmental conditions, and sexual reproduction. To date, no genome-wide overview of the Brassica rapa nsLtp (BrnsLtp) gene family has been performed. Therefore, as the first step and as a helpful strategy to elucidate the functions of BrnsLtps, a genome-wide study for this gene family is necessary. Methodology/Principal Finding In this study, a total of 63 putative BrnsLtp genes were identified through a comprehensive in silico analysis of the whole genome of B. rapa. Based on the sequence similarities, these BrnsLtps was grouped into nine types (I, II, III, IV, V, VI, VIII, IX, and XI). There is no type VII nsLtps in B. rapa, and a new type, XI nsLtps, was identified in B. rapa. Furthermore, nine type II AtLtps have no homologous genes in B. rapa. Gene duplication analysis demonstrated that the conserved collinear block of each BrnsLtp is highly identical to those in Arabidopsis and that both segmental duplications and tandem duplications seem to play equal roles in the diversification of this gene family. Expression analysis indicated that 29 out of the 63 BrnsLtps showed specific expression patterns. After careful comparison and analysis, we hypothesize that some of the type I BrnsLtps may function like Arabidopsis pathogenesis-related-14 (PR-14) proteins to protect the plant from phytopathogen attack. Eleven BrnsLtps with inflorescence-specific expression may play important roles in sexual reproduction. Additionally, BrnsLtpI.3 may have functions similar to Arabidopsis LTP1. Conclusions/Significance The genome-wide identification, bioinformatic analysis and expression analysis of BrnsLtp genes should facilitate research of this gene family and polyploidy evolution and provide new insight towards elucidating their biological functions in plants.
ASSSESSING LINGUISTIC COMPLEXITY

The question of “linguistic complexity” is interesting and fruitful. Unfortunately, the intuitive meaning of “complexity” is not amenable to formal analysis. This paper discusses some proposed definitions and shows how complexity can be assessed in various frameworks. The results show that, as expected, languages are all about equally “complex,” but further that languages can and do differ reliably in their morphological and syntactic complexities along an intuitive continuum. I focus not only on the mathematical aspects of complexity, but on the psychological ones as well. Any claim about “complexity” is inherently about process, including an implicit description of the underlying cognitive machinery. By comparing different measures, one may better understand human language processing and similarly, understanding psycholinguistics may drive better measures.
SNPs on human chromosomes 21 and 22 -- analysis in terms of protein features and pseudogenes.

SNPs are useful for genome-wide mapping and the study of disease genes. Previous studies have focused on SNPs in specific genes or SNPs pooled from a variety of different sources. Here, a systematic approach to the analysis of SNPs in relation to various features on a genome-wide scale, with emphasis on protein features and pseudogenes, is presented. We have performed a comprehensive analysis of 39,408 SNPs on human chromosomes 21 and 22 from the SNP consortium (TSC) database, where SNPs are obtained by random sequencing using consistent and uniform methods. Our study indicates that the occurrence of SNPs is lowest in exons and higher in repeats, introns and pseudogenes. Moreover, in comparing genes and pseudogenes, we find that the SNP density is higher in pseudogenes and the ratio of nonsynonymous to synonymous changes is also much higher. These observations may be explained by the increased rate of SNP accumulation in pseudogenes, which presumably are not under selective pressure. We have also performed secondary structure prediction on all coding regions and found that there is no preferential distribution of SNPs in a -helices, b -sheets or coils. This could imply that protein structures, in general, can tolerate a wide degree of substitutions. Tables relating to our results are available from http://genecensus.org/pseudogene.
Quality of life and functional capacity of elderly women with knee osteoarthritis

ABSTRACT Objective: To correlate functional ability and quality of life of elderly women with knee osteoarthritis. Methods: Cross-sectional study composed of 40 elderly women with knee osteoarthritis. We used the following instruments: identification questionnaire, Western Ontario and McMaster Universities Arthritis Index (WOMAC), and World Health Organization Quality of Life Questionnaire-OLD (WHOQOL-OLD). The significance level was 0.05 (5%), and confidence intervals were 95%. For statistical analysis we used parametric statistical tests, descriptive analysis, test for equality of two proportions, Pearson's correlation, correlation test, and analysis of variance. Results: The mean age (± standard deviation) was 74.1 (±6.7) years, and 47.5% of patients had osteoarthritis in both knees. Moderate pain was reported by 45% of patients when they walked on a flat plane and 40% when they were seated or lying down; 55% had severe or very severe pain when climbing or descending stairs; 50% reported moderate joint stiffness after sitting, lying, or resting; and 65% reported moderate or little stiffness after waking. In physical function, 60% of patients had moderate or severe difficulty in descending stairs and 67.5%, when climbing stairs; 60% reported severe or very severe difficulty in getting in and out of the car, and 70%, when performing strenuous housework. The correlation with WHOQOL-OLD and WOMAC was negative and not significant except for autonomy, which was significant. Sedentary and elderly women who used walking aid devices had worse WOMAC functional capacity, but this finding was not statistically significant. In WHOQOL-OLD, volunteers scored higher on social participation and engagement in physical activity on autonomy, which was statistically significant compared with the nonvoluntary and sedentary domains, respectively. Conclusion: It is possible to have a good quality of life even with functional impairment from knee osteoarthritis.